LOCUS       NZ_JAEVFI010000046     13308 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Streptomyces sp. JJ66 scaffold46_size19429, whole genome shotgun
            sequence.
ACCESSION   NZ_JAEVFI010000046
VERSION     NZ_JAEVFI010000046.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. JJ66
  ORGANISM  Streptomyces sp. JJ66
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 19429)
  AUTHORS   Jose,P.A. and Jha,B.
  TITLE     Draft genome of Streptomyces sp. JJ66
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19429)
  AUTHORS   Jose,P.A. and Jha,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2021) Marine Biotechnology and Ecology Division,
            CSIR - Central Salt and Marine Chemicals Research Institute, G.B.
            Marg, Bhavnagar, Gujarat 364002, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-MAY-2017
            Assembly Method                   :: Velvet v. V 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 320.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019303495.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 03:51:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,446
            Genes (coding)                    :: 4,340
            CDSs (with protein)               :: 4,340
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 61
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 31 of 106
            Pseudo Genes (incomplete)         :: 82 of 106
            Pseudo Genes (internal stop)      :: 3 of 106
            Pseudo Genes (multiple problems)  :: 9 of 106
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:22:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6121
            Orig. end                         :: 19429
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAEVFI010000046.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13308
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..13308
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13308
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[10000:12636](-)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12636)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    1246..1259
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.84"
                     /tool="antismash"
     gene            1363..2802
                     /locus_tag="JJV70_RS21685"
                     /old_locus_tag="JJV70_21670"
     CDS             1363..2802
                     /GO_function="GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146062.1"
                     /locus_tag="JJV70_RS21685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_21670"
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="WP_219049735.1"
                     /transl_table=11
                     /translation="MRFLNDQRPPYDLTYDDVFMVPSRSAVGSRQGVDLSSHDGSGTTI
                     PLVVANMTAIAGRRMAETVARRGGLVVIPQDIPLDVVSDVVSWVKQRHLVLDTPIVLAP
                     HATVADALSLLPKRAHGAGVVVEDGRPVGVVTESDLTGVDRFTQLYQVMSKDLLVLDAG
                     VDPQEAFTRLDSAHRRLAPAVDADGRLVGILNRKGALRATLYTPATDADGRLRVAAAVG
                     INGDVAGRAKALLEAGADTLVVDTAHGHQEGMLQALKAVRALDPRVPVVAGNVVAAEGV
                     RDLIEAGADIVKVGVGPGAMCTTRMMTGVGRPQFSAVLECAAEARKHGKHVWADGGVRH
                     PRDVAMALAAGASNVMVGSWFAGTHESPGDLQQAADGRLYKESFGMASARAVRNRTAEE
                     SAYDRARKGLFEEGISHSRMFLDPARPGVEDLIDSIIAGVRSSCTYAGADSLAQFAERA
                     IVGVQSAAGYAEGKPLHASWS"
     misc_feature    complement(3008..3021)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.04"
                     /tool="antismash"
     misc_feature    complement(3054..3068)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.17"
                     /tool="antismash"
     gene            3217..4692
                     /locus_tag="JJV70_RS21690"
                     /old_locus_tag="JJV70_21675"
     CDS             3217..4692
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1038: phenylalanine-specific permease"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011027796.1"
                     /locus_tag="JJV70_RS21690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_21675"
                     /product="amino acid permease"
                     /protein_id="WP_219049736.1"
                     /transl_table=11
                     /translation="MLDPTTAPPAPSAPPGGRFLRRKPVERLVAESGHGSEGGSLRRTL
                     SMWQLTMISIGATLGTGIFVVLGEAVPLAGPAVTISFVIAGLTALFSALSYAELAGTIP
                     ASGSSYSYTYATMGELVAWVCGLCLILEYGVSVAAVAVGWGQYLNEFLDGTIGVTIPAA
                     LSNPPGDGGYFNLPALLVVVLAMVFLLGGAKESARANAIMVFVKIAALLMFCAVGFTGV
                     SSGNYEDFMPLGIAGVSAAGASLFFSYIGFDAASTAGEEAKNPQRDLPRAIMLSLLIVT
                     VLYVLVAAVAVGAVPWQSFEGSEAALAGVLSDVTGQTFWATLLAAGAVIAIASVVLAVM
                     YGQTRILFAMSRDGLVPRVFSKVHPRTGSPRMNTLLVAAFCGILAAAIPLGELANATSI
                     GTLFAFALVNISVIVLRRTRPDMPRSFRVPLSPVLPLLGLGFCIYNMVNLPAITWLVFG
                     LWMAAGLVFYFGYGQRRSRLAVEAHEPPATAEK"
     gene            4703..5158
                     /locus_tag="JJV70_RS21695"
                     /old_locus_tag="JJV70_21680"
     CDS             4703..5158
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1120: AsnC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016645535.1"
                     /locus_tag="JJV70_RS21695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_21680"
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="WP_219049737.1"
                     /transl_table=11
                     /translation="MRLNQLDERIVHALAEDARRSYADIGAEVGLSAPAVKRRVDRLRA
                     DGAITGFTVRVDPAALGWETEGFIEMYCRHNTSPEDIRRGLSRYPEVASASTVTGDADA
                     IVQIYAADMRHFERVLERIAGEPFVERTKSVLVLSPLLRRYTASSPA"
     misc_feature    complement(5412..5421)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.9"
                     /tool="antismash"
     gene            5500..6324
                     /locus_tag="JJV70_RS21700"
                     /old_locus_tag="JJV70_21685"
     CDS             5500..6324
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018570177.1"
                     /locus_tag="JJV70_RS21700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_21685"
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="WP_219049738.1"
                     /transl_table=11
                     /translation="MTPLRIALYQGPGGVPTPPQALDAFAHAARRAADAGARLLVTPEL
                     SLTGYALDPAVLAERAEAADGPAAHAVGKIAAEYGVAVHYGYPERSEDGAVHNSAQLIG
                     PDGTPLANYRKTHLYGDFERARFRPGDQPVVQADLDGVRVGLLVCYDIEFPEPVRAHAL
                     AGTELLLAPTALMRPYEIVPRQVVPVRAWENQLYLAYVNRHGREGDFDFAGMSCLVAPD
                     GTVRARAGSGEDLVLADVEHAALAACRAENTYLTDRRPELYPSLSHRGEPRR"
     gene            6321..8039
                     /locus_tag="JJV70_RS21705"
                     /old_locus_tag="JJV70_21690"
     CDS             6321..8039
                     /EC_number="1.-.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671499.1"
                     /locus_tag="JJV70_RS21705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_21690"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_219049739.1"
                     /transl_table=11
                     /translation="MTSTVPAAVHDEQHAEAQSAPPITMFGPDFPFAYDDFLAHPAGLG
                     QVPATEHGTEVAVIGGGLSGIVTAYELMKMGLRPVVYEADQIGGRLRTDVFEGSDDGTG
                     EPLTAEMGAMRFPPSSTAFQHYIDLVGLDVKPFPNPLAADTPSTVVDLKGESHYARTLD
                     DLPEVYHQVMNAWNACLEEGADFSDMQRAIRERDVPRIREIWARLVEKLDNQTFYGFLC
                     DSEAFASFRHREIFGQVGFGTGGWDTDFPNSILEILRVVYTGADDDHRGIIGGSQQLPL
                     RLWEREPDKLVHWPKGTSLASLHGGEPRPAVTRMHRTAGNRITVTDARGDIRTYQAAVF
                     TAQSWMMLSKIDCDDSLLPIDHWTAVERTHYMESSKLFVPVDRPFWLDTDEETGRDVMS
                     MTLTDRMTRGTYLLDNGPDQPAVICLSYTWCDDSLKWLPLDANERMEVMLKSLREIYPK
                     VDIRQHITGNPVTVSWENEPYFMGAFKANLPGHYRYQRRLFTHFMQDRLPQDKRGLFLA
                     GDDISWTAGWAEGAVQTALNAVWGVMHQLGGATDPANPGPGDVYDEIAPVELPED"
     gene            complement(8128..9996)
                     /locus_tag="JJV70_RS21710"
                     /old_locus_tag="JJV70_21695"
     CDS             complement(8128..9996)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047205.1"
                     /locus_tag="JJV70_RS21710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_21695"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_308294953.1"
                     /transl_table=11
                     /translation="MSATTLPGPPPAAPGGPPEEPRPTPAPSSGQGGQASGRRALLASL
                     RERWGWLSAGVLLGLVGSVMGLAQPLAAKRVVDGMGRGEAVTGAVLLLSALVVAGALLA
                     GLGHYVLQRTAESLVREVRHRLSARLLGMRVGAAEKADPGDLISRVTSDTTVLRQAAPE
                     ALAAAVTGTLVTSATLVLMCVLDAVLFCVTLAVIALVSVLIAVTAPRIGSATRRTQEAV
                     GELGAALERVLGAFRTVKAATAEHHEATRLDAAADRACRAGVKAAAWQAVSVTSSAVVM
                     QVCFLVVLGVGGARVASGAISVSTLVAFLLYVFALAPRLSQLVEGVGHFQVGAAAALRI
                     EQVTQLEVEHGGPPGAPAIATAGQPPATATPPDGSPLTVRFDRVRFAYRPGLPPAHRGV
                     SFTVPARGLTAFVGPSGAGKTTVFSLIARFHDPDTGRVLLDRQDAREWPLHRLRTALGY
                     VEQDAPVLAGTLRDNLLLGPVEASEAELARVLRLTRLDVLAARLPQGLDTSVGHRGGTL
                     SGGERQRVAVARALLRRPRLLLLDEATSQLDAVNEAALRDAVHAASRSTTVLVIAHRLS
                     TVVRADQIIVMEDGRVRATGTHAQLMARDSLYAELARGQLLHPEEP"
     gene            complement(10001..10417)
                     /locus_tag="JJV70_RS21715"
                     /old_locus_tag="JJV70_21700"
     CDS             complement(10001..10417)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047207.1"
                     /locus_tag="JJV70_RS21715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_21700"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_219049741.1"
                     /sec_met_domain="PF13471 (E-value: 7.6e-26, bitscore: 80.7,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTPEAVPYAPHLVPPATRWAARVVVVGAHLLATQPPARISAVLR
                     RLRGRVRPATFQEANAARETILAVSLAAGGQKGCLARSLATVLLCRTRGQWVTWCVGVR
                     TRPPFAAHAWVEAEGVLVGEGASADYFQRLLTVE"
     gene            complement(10475..10732)
                     /locus_tag="JJV70_RS21720"
                     /old_locus_tag="JJV70_21705"
     CDS             complement(10475..10732)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986678.1"
                     /locus_tag="JJV70_RS21720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_21705"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_219049742.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 3.5e-23,
                     bitscore: 71.7, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 3.2e-16, bitscore: 49.7,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATLKSGVLLAETEYGMALLDQTSAEYWTLNPTGVVILRALLDGR
                     GLPEAVDDLTARYDGLDRTEAERDARDLLAELRTAGLLRP"
     aSDomain        complement(10481..10729)
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_JJV70_RS21720_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="5.70E-23"
                     /identifier="RREFam002.1"
                     /label="JJV70_RS21720"
                     /locus_tag="JJV70_RS21720"
                     /protein_end="84"
                     /protein_start="1"
                     /score="71.7"
                     /tool="antismash"
                     /translation="ATLKSGVLLAETEYGMALLDQTSAEYWTLNPTGVVILRALLDGRG
                     LPEAVDDLTARYDGLDRTEAERDARDLLAELRTAGLLR"
     gene            complement(10738..12636)
                     /locus_tag="JJV70_RS21725"
                     /old_locus_tag="JJV70_21710"
     CDS             complement(10738..12636)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986679.1"
                     /locus_tag="JJV70_RS21725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_21710"
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /protein_id="WP_219049743.1"
                     /sec_met_domain="Asn_synthase (E-value: 5.7e-35, bitscore:
                     111.7, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRSTSFPALSAWFAVLPDTEEGAHLAAGLGSTPLNLSHASGRPW
                     VVGSRAPEGIAVGSHGDTRVAVIGEHRVTPEQAARAAAGGPAAWGEAGRMWPGSFHLLV
                     SEAGRVRVQGSAVGLRRVFYGTVPGPGRCVLAADRADVIAELTGAGLDERRVAVELLTS
                     GAPHPLTGAPLWHGVHALPSGHRLTLEPGSRTAVSRWWHVPSSEVPLSEGAEGLRTELR
                     AAVEVRTHGRSLVTTDLGGLDSTAVCCTAARGPATVAAYTAALHDQAGDDVYWAERTVQ
                     ELPGVEHHIVPAADVAMTFEGVDSLQDVLDTPSIITVDRNRRMGIIERAAARGSGLHLC
                     GLGGDELLAGTPAHLHSLLRTHPRTGLRALRGYTAKYRWPYSAVLRQLGDRRPYRAWLA
                     RVADDLTAPAPAADEPLLDWAAPPRMPPWATGDARAAARELLRAELPTAQPLAPGHGTH
                     RELAAMEGISQWARHIGQMAAPLGLTFSAPYYDVRVIEAALAVRPHERITPWRYKPLIV
                     EAMRGVVPESSRTRGTKANATYEEEAGLRAHRAGLLALCEDSRLARLGLVDEGELRRWC
                     LRALSAESESYQLHPTLACEVWLRTRERLASQPPEAVRQPEQTAGHSRPAATPTER"
     gene            complement(12787..12894)
                     /locus_tag="JJV70_RS21730"
                     /old_locus_tag="JJV70_21715"
     CDS             complement(12787..12894)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039305.4"
                     /locus_tag="JJV70_RS21730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_21715"
                     /product="keywimysin-related RiPP"
                     /protein_id="WP_257574365.1"
                     /transl_table=11
                     /translation="MTYERPTLAKAGGFRKVTGLKDRGPKDVLGGKQLL"
ORIGIN
        1 attctcggcc gcttcgccgg gcgcatgggc atgtccccga tcccgctgta cctgctgtgc
       61 gggctcgcct tcggccacgg cgggctgctg ccgctggccg ccagcgaggg gttcttcgag
      121 gtcggcgccg agatcggcgt catcttgctg ctgctcatgc tggggctgga gtacagcgcc
      181 tcggagctgg tgacgaacct gcgcacgcag tacccctcgg gcctggttga cttcctgctc
      241 aacgccacgc cgggcgcgct cgcgggctgg gcgctgggct gggagccggt gggcatcatc
      301 gcgctcgcgg gcgtcacctg gatctcgtcc tccggcgtca tcgccaaggt gctcaccgac
      361 ctgggccggc tgggcaaccg ggagacgccg gtagtcctcg gcgtgctggt catggaagac
      421 ctcgccatgg ctggctacct gccgctgctc accgcgctgc tgaccggcgc ctcgctggcg
      481 ggcgccagcc tcacgctgac gatcgcgctg ggcaccgccg gactcgtgct cttcctcgcc
      541 ctgcggcacg gacggctgat cagccgcgcg gtgtcctcgg acaaccccga gctgctgctg
      601 ctggccgtcc tcgggctcac cctgctcgtg gcgggtgtcg cccagcggct tcaggtctcg
      661 gcggccgtcg gcgccttcct cgtcggcatc gccatctccg gcgaggtcgc gcacagcgcc
      721 cgcgcgctgc tcacgccgct gcgcgacctg ttcgcggccg tgttcttcgt cttcttcggc
      781 ctgtccaccg atccggccaa catccccccg gtgctgctgc ccgcgctgct gctggccgtc
      841 gtctccgtcg gaacgaagat cgtcactggc tggtacgcgg cccggcgcgc ccggatcggg
      901 gccagagggc ggctgcgggc gggcggggcg ctggtggcgc gcggtgagtt ctccgtcgtc
      961 atcgccgggc tcgccgccgg ggcgcaaccc gggctcgcgc ccctggccac ggcgtacgtc
     1021 ctgctgctcg tcatcgccgg tccgctcgcc gcccgctgga cggaaccagc ggtgcgccgg
     1081 gtcttccggc gccccgccgc cccgcccccg gccaccacgg cgcccacccc cggcccggcc
     1141 gccgcccccc gctccggacg gcggacctga cgccggtcag gcccctcccg cgctttcagc
     1201 gccgtgacgt ggctctcgta gaaaccgacg gcccgtcctc cctacgtgtg gcggatcaaa
     1261 cgggggcctg ggggccggtg gccatgggag aatgaagtac gtgcgttttt ccccggccgg
     1321 ccaccgccgg gggtctcctc cgatcgactg ggatgtctgc acgtgcgatt cctcaacgac
     1381 cagcggccgc cgtacgacct gacgtatgac gacgtcttca tggtgccgag ccgttcggct
     1441 gtcggctccc gccagggcgt ggacctctcc agccacgacg ggagcggcac caccatcccg
     1501 ctcgtcgtgg ccaacatgac cgccatcgcg ggccgccgga tggccgagac cgtcgcccgg
     1561 cgcggcggcc tcgtcgtcat cccgcaggac atcccgctcg atgtcgtcag cgacgtcgtc
     1621 tcctgggtca agcagcgcca tctggtgctg gacaccccga tcgtgctcgc gccgcacgcc
     1681 accgtcgccg acgcgctgtc gctgctgccc aagcgggccc acggcgccgg tgtggtcgtc
     1741 gaggacgggc gtccggtcgg cgtggtcacc gagtccgacc tcaccggcgt cgatcgcttc
     1801 acccagctct accaggtgat gtcgaaggac ttgctggtcc tggacgcggg cgtcgatccg
     1861 caggaggcgt tcacccgcct ggacagcgcc caccgcaggc tcgccccggc cgtggacgcc
     1921 gacggccgcc tggtcggcat cctcaaccgc aagggcgccc tgcgggccac gctgtacacc
     1981 ccggccaccg acgctgatgg ccgcctgcgc gtcgccgccg ccgtcggcat caacggcgac
     2041 gtcgcgggcc gggcgaaggc cctgctggag gcaggcgcgg acaccctcgt cgtggacacc
     2101 gcccacggcc accaggaggg catgctccag gcgctgaagg cggtgcgcgc gctcgacccg
     2161 cgggtaccgg tcgtcgcggg caacgtggtg gccgccgagg gtgtgcgcga cctgatcgag
     2221 gcgggcgccg acatcgtcaa ggtgggcgtc ggcccgggcg ccatgtgcac cacccgcatg
     2281 atgaccggcg tcggacggcc gcagttctcg gcggtgctgg agtgcgccgc cgaggcgagg
     2341 aagcacggca agcacgtgtg ggccgacggc ggcgtgcggc acccgcgcga cgtggcgatg
     2401 gcgctggcgg ccggtgcctc caatgtcatg gtcggctcct ggttcgccgg tacccacgag
     2461 tcgcccggcg acctccagca ggccgctgac gggcgcctgt acaaggagtc cttcggcatg
     2521 gcctccgccc gcgccgtgcg caaccgcacg gccgaggagt ccgcctacga ccgcgcccgc
     2581 aagggcctgt tcgaggaggg catctcgcac tcgcggatgt tcctcgaccc ggcccggccc
     2641 ggcgtggagg acctgatcga ctcgatcatc gcgggcgtgc gctcctcgtg cacctacgcc
     2701 ggtgccgact ccctggcgca gttcgccgag cgcgcgatcg tcggcgtgca gagcgccgcg
     2761 ggctatgcgg agggcaagcc gctgcacgcc agctggagct gacgcccgcg tcagtgacgg
     2821 gagacccgcg aggggagatc cggcgcagaa gatctggagc agaagagggg cacggccggt
     2881 ggccgtgtcc ctctcggcgt ttgggcggtc agcggcccgg gcgcgggccg gatgcgattt
     2941 gcctccgggg cgcgttacca caagaatcct gtcaggggaa gtaaacgaac atgcgaaccc
     3001 gggtccattt gcgcaacaaa tctccgggtg tgcgcaaggg tgacccgtta cgtccctacc
     3061 tgtggtgctc gtagagtcat gcgctgtacg aggagtggcg gagaccgcct gctcggtctt
     3121 cagccgctgt gtcgtgatca tgccgatccg gtgcggccct gagccgtgcc gggctgccgc
     3181 cacagacccg cagcaacatc ggcaaggagc cgccccgtgc tggaccccac cacagcgccc
     3241 cccgcaccgt ccgcgccccc ggggggccgg ttcctgcggc gcaagcccgt ggagcgattg
     3301 gtggccgagt ccggccacgg cagcgagggc ggcagcctgc gccgcaccct gtcgatgtgg
     3361 cagctgacca tgatcagtat cggtgccacg ctgggtaccg gcatcttcgt cgtgctcggc
     3421 gaggccgttc cgctggccgg gcccgccgtc acgatctcct tcgtgatcgc cggtctgacc
     3481 gcgctgttct ccgcgctgtc ctacgccgag ctggccggca ccattcccgc ctcgggctcc
     3541 tcctactcct acacctacgc caccatgggt gaactggtcg cctgggtgtg tggcctgtgc
     3601 ctgatcctgg agtacggcgt ttccgtcgcc gccgtcgccg tcggctgggg ccagtacctc
     3661 aacgagttcc tcgacggcac catcggcgtc accatccccg ccgcgctgtc caacccgccc
     3721 ggtgacggcg gctacttcaa cctgcccgcg ctgctcgtcg tcgtcctggc gatggtcttc
     3781 ctgctgggcg gcgccaagga gagcgcccgg gccaacgcga tcatggtgtt cgtgaagatc
     3841 gccgcgctgc tgatgttctg cgccgtgggc ttcaccggcg tcagctccgg caactacgag
     3901 gacttcatgc cgctgggcat cgcgggcgtc agcgccgcgg gcgcctcgct gttcttctcc
     3961 tacatcggct tcgacgccgc ctccaccgcc ggtgaggaag ccaagaaccc gcagcgggac
     4021 ctgccgcgcg cgatcatgct ctcgctgctg atcgtcaccg tcctgtacgt gctcgtcgcc
     4081 gccgtcgccg tcggtgccgt gccctggcag agcttcgagg gctccgaggc cgcgctcgcc
     4141 ggggtgctga gcgacgtcac cggccagacg ttctgggcca ccctgctggc cgccggtgcc
     4201 gtcatcgcca tcgccagcgt cgtgctggcc gtcatgtacg gccagacccg catcctgttc
     4261 gccatgtccc gcgacggcct ggtgccccgc gtcttctcca aggtgcaccc ccgcaccggc
     4321 tcgccgcgca tgaacacgct gctcgtggcc gcgttctgcg gcatcctggc cgccgcgatc
     4381 ccgctgggtg agctggccaa cgccaccagc atcggcacgc tgttcgcctt cgcgctggtc
     4441 aacatctcgg tgatcgtcct gcgccgcacc cgccccgaca tgccgcgcag cttccgcgtg
     4501 ccgctctcgc cggtcctgcc gctgctgggc ctgggctttt gcatctacaa catggtgaac
     4561 ctgcccgcca tcacctggct ggtgttcgga ctctggatgg ccgccgggct cgtgttctac
     4621 ttcgggtacg gccagcgccg ctcccggctg gccgtcgagg cccacgaacc tcccgcgacc
     4681 gcagagaagt gatccacccg ccgtgcgact gaaccagctc gacgaacgca tcgtccacgc
     4741 cctcgctgag gacgcccgcc gctcctacgc cgacatcggc gccgaggtgg ggctctccgc
     4801 ccccgccgtc aagcgccgcg tggaccggct gcgcgccgac ggcgccatca ccggcttcac
     4861 cgtccgggtg gacccggcgg ccctcggctg ggagacggag gggttcatcg agatgtactg
     4921 ccgccacaac acctctcccg aggacatccg gcggggtctg tcgcgctacc cggaggtggc
     4981 gtccgcgtct accgtcaccg gcgacgcgga cgccatcgtc cagatctacg cggccgacat
     5041 gcggcacttc gagcgcgtgc tggagcggat cgcgggcgag ccgttcgtgg aacgcacgaa
     5101 gtcggtcctc gtcctctccc cgctcctgcg ccgctacacc gccagctccc cggcgtaggc
     5161 cctggtaccg gaccgagcct ccggccgggg gaccccgcct tcccggaacc gggcggatgc
     5221 gcccccgggc gtttcaactt cccgccgtgc ggtggtccgg cacctccggc tccgggccgc
     5281 agcaccccgg acgggctggc ttccccagcc cgtccggggc acctcccagc gggcagcaaa
     5341 gggagaggct gggcctgtgc aacgaatcgc cgcagccggg acaacacacg caacgaatac
     5401 ctcatgggta cgcaaaaaac gccgcttgtc cgccacgtgg cgcgaaacgt acggtcggac
     5461 tatcccactc acaccggttg acggaagccg cgaggtccca tgacgcccct gcgcatcgcg
     5521 ctgtaccaag gccccggcgg cgtgcccacg ccgccgcagg ccctcgacgc gttcgcgcac
     5581 gccgcccgcc gggccgccga cgccggagcg cggctgctgg tcacccccga gctgtccctg
     5641 accgggtacg cgctggaccc ggccgtgctc gccgagcgcg cggaggcggc cgacggcccc
     5701 gccgcccacg ccgtcggcaa gatcgccgcc gagtacggcg tcgccgtcca ctacggctac
     5761 cccgagcgct cagaagacgg cgccgtccac aactccgcgc agctcatcgg tcccgacggc
     5821 acaccgctgg ccaactaccg caagacccac ctgtacggcg acttcgagcg cgcccgtttc
     5881 cgccccggtg accagccggt cgtccaggcc gacctcgacg gcgtccgcgt cggcttgctg
     5941 gtgtgctacg acatcgagtt ccccgagccg gtacgggccc acgcgctggc gggcaccgag
     6001 ctgctgctgg cgccgacggc gctgatgcgg ccgtacgaga tcgtgccccg gcaggtcgtg
     6061 cccgtgcggg cgtgggagaa ccagctgtac ctcgcctacg tcaaccgcca cggccgcgaa
     6121 ggcgacttcg acttcgccgg gatgagctgc ctggtcgccc ccgacggcac cgtgcgcgcc
     6181 cgcgcgggca gcggcgagga cctggtcctc gccgacgtcg agcacgccgc gctggccgcc
     6241 tgccgggccg agaacaccta cctcaccgac cgccgtcccg agctgtaccc gtccctgtcc
     6301 caccgaggag aaccgcgccg atgacctcca ccgtgcccgc cgccgtccac gacgagcagc
     6361 acgccgaggc ccagtccgcc ccgcccatca cgatgttcgg tccggacttt cccttcgcct
     6421 acgacgactt cctcgcccac ccggcgggcc ttggccaggt gccggccacc gagcacggca
     6481 ccgaggtcgc cgtcatcggc ggtggcctgt ccggcatcgt cacggcctac gagctgatga
     6541 agatgggcct gaggcccgtc gtctacgagg ccgaccagat cggtggccgc ctgcgcaccg
     6601 acgtgttcga gggctccgac gacggcaccg gcgagccgct gacggccgag atgggcgcga
     6661 tgcgcttccc gccgtcctcc accgccttcc agcactacat cgacctcgtc ggcctggacg
     6721 tcaagccgtt cccgaacccg ctggcggccg acaccccgtc caccgtggtg gacctcaagg
     6781 gcgagagtca ctacgcgcgc accctggacg acctgcccga ggtctaccac caggtgatga
     6841 acgcctggaa cgcctgtctg gaggagggtg ccgacttctc cgacatgcag cgcgccatcc
     6901 gggagcgcga cgtgccgcgc atccgggaga tctgggcccg gctggtggag aagctggaca
     6961 accagacctt ctacggcttc ctgtgcgact ccgaggcgtt cgcctccttc cggcaccggg
     7021 agatcttcgg ccaggtcggc ttcggcaccg gcggctggga caccgacttc cccaactcca
     7081 tcctggagat cctgcgcgtc gtctacaccg gcgccgacga cgaccaccga ggcatcatcg
     7141 gcggcagcca gcagctgccg ctgcggctgt gggagcgcga gccggacaag ctggtgcact
     7201 ggccgaaggg cacctcgcta gcgtccctgc acggcggcga gccgcgcccg gccgtcaccc
     7261 gcatgcaccg caccgcgggc aaccgcatca ccgtcaccga cgcccggggc gacatccgca
     7321 cctaccaggc ggccgtcttc accgcgcagt cgtggatgat gctctccaag atcgactgcg
     7381 acgactcgct gctgcccatc gaccactgga cggccgtcga gcgcacgcac tacatggagt
     7441 cctccaagct gttcgtcccc gtggaccggc cgttctggct ggacaccgac gaggagaccg
     7501 gccgggacgt gatgagcatg acgctcaccg accgcatgac ccgcggcacc tacctgctgg
     7561 acaacggccc ggaccagccc gccgtcatct gcctgtccta cacctggtgt gacgacagcc
     7621 tcaagtggct cccgctggac gcgaacgagc gcatggaggt catgctcaag tcgctgcgcg
     7681 agatctaccc gaaggtggac atccgccagc acatcaccgg caacccggtg accgtctcct
     7741 gggagaacga gccctacttc atgggcgcgt tcaaggcgaa cctgcccggc cactaccgct
     7801 accagcgccg cctgttcacc cacttcatgc aggaccgcct gccccaggac aagcgcggcc
     7861 tgttcctcgc cggggacgat atctcctgga cggccggctg ggccgaaggc gcggtgcaga
     7921 cggcgctcaa cgcggtctgg ggcgtgatgc accagctcgg cggcgccacc gacccggcca
     7981 accccggccc cggtgacgtc tacgacgaga tcgccccggt ggaactgccg gaggactgac
     8041 ccgccacacc cgaagcgggg cgggaccagc acgggcccgc cccgctcagc cgtgctccgg
     8101 cctctcccgg gcgtcgtgga cgcggggtca gggttcttcg gggtggagga gctggccgcg
     8161 ggctagctcc gcgtagaggc tgtcgcgggc catcagctgg gcgtgggtcc ctgtcgcccg
     8221 gacgcggccg tcttccataa cgatgatctg gtcggctctg accaccgtgg acagccggtg
     8281 ggcgatgacc agcacggtgg tactgcggga agcagcgtgc accgcgtccc gcagcgccgc
     8341 ctcgttgacc gcgtccagct gcgaggtggc ctcgtccagc agcaacagcc ggggacggcg
     8401 caacagggcc cgggcgacgg ctacgcgctg gcgttcccct ccggacaggg tgccaccacg
     8461 gtgtcccacc gaggtgtcca gtccctgggg cagccgcgcg gccagcacgt ccagccgggt
     8521 cagccgcagc acccgcgcca gttcggcttc gctggcttcg accggtccca ggagcaggtt
     8581 gtcgcgcagc gtcccggcga ggaccggcgc gtcctgctcc acgtacccca gggccgtgcg
     8641 cagccggtgc aagggccact cgcgcgcgtc ctgacggtcc agcaggaccc gcccggtgtc
     8701 cgggtcgtgg aagcgtgcga tcagggagaa gacggtggtc ttccccgctc ccgagggccc
     8761 cacgaaggcg gtcaggccac gggcgggaac ggtgaaggag acgccccggt gggccggtgg
     8821 cagcccgggc cggtaggcga agcgcacccg gtcgaaccgt acggtgagcg gcgacccgtc
     8881 cggcggcgtg gcggtggccg gcggctgccc ggcggtcgcg atggctggcg cgcctggcgg
     8941 tccgccgtgc tccacctcca actgcgtcac ctgctcgatg cggagcgcgg cagccgcccc
     9001 tacctggaag tgccccacgc cctccaccag ctggctcagc cggggtgcga gcgcgaaaac
     9061 atacagcaag aaggccacga gcgtggagac gctgatggct cctgaggcga cccgggcgcc
     9121 gcccaccccc agcacgacga ggaaacagac ctgcatgacc accgcggagg aggtgaccga
     9181 aaccgcctgc cacgccgcgg ccttcacccc cgcgcggcac gcccggtcag ccgcggcgtc
     9241 cagcctggtg gcttcgtggt gctccgcggt ggcggccttg acggtgcgga acgcccccag
     9301 cacgcgttcc agcgcggccc ccagttcgcc caccgcttcc tgggtgcggc gggtggcgct
     9361 gccgatgcgc ggcgcggtca ccgcgatcag cacgctcacc agggcgatca cggcgagggt
     9421 gacgcagaac agcacggcgt ccaggacaca catcagcacc agggtcgccg aggtgaccag
     9481 cgtgccggtg accgccgcgg ccagggcctc gggcgcggct tgccgcagca cggtggtgtc
     9541 ggaggtgacc cgcgagatca gatctccggg gtcggccttc tcggccgccc ccacccgcat
     9601 ccccagcagc cgggccgaaa gccggtgccg cacctcgcgc accagcgact cggcggtccg
     9661 ctggagcacg tagtgcccca gccccgccag cagcgcaccg gccacgacca gggcactgag
     9721 cagcagcacg gccccggtga cggcttcacc ccggcccatg ccgtccacca cccgcttggc
     9781 cgcgaggggc tgggccagac ccatgacgct gcccacgagc ccgaggagga cgcccgcgct
     9841 gagccagccc caccgctcac gcagggaggc cagcagcgcc cggcgcccgc tggcttgccc
     9901 accctgcccg ctggacggcg ccggggtcgg gcggggctcc tccggcgggc cgcctggcgc
     9961 cgccggcggg ggaccgggga gggtggtggc gctcaccgcc tcactccacc gtgagcaggc
    10021 gctggaagta gtccgccgaa gcgccctcgc cgacgagaac gccctccgct tccacccagg
    10081 catgggcggc gaagggcgga cgggtgcgga cccccacgca ccaggtgacc cactgccccc
    10141 gcgtccggca cagcaggacg gtggccaggg agcgggccag acagcccttc tggcctccgg
    10201 ccgccaggct gacggcgagg atggtctcgc gggcggcgtt cgcctcctgg aacgtggcgg
    10261 gccggacccg gccgcgcagt cggcgcagta ccgcgctgat gcgggccgga ggctgggtgg
    10321 cgagcaggtg cgcgcccacc accaccaccc gtgcggccca ccgggtagcc ggcgggacca
    10381 agtgcggtgc gtacggcacc gcttccggcg ttgacatctc gtcctgcctt tcacgtatcg
    10441 ggttctggtg gcggtagggg cggacggact ggcctcaggg cctcagcagt ccggcggtgc
    10501 gcagttcggc gagcagatcc cgggcgtccc gctccgcctc ggtgcggtcg aggccgtcgt
    10561 accgggcggt caggtcgtcc accgcctcgg gcaggccgcg cccgtccagc agggcgcgca
    10621 ggatcaccac cccggtcggg ttgagcgtcc agtactcggc ggaggtctgg tccagcagag
    10681 ccatgccgta ttccgtttcg gcgagcagga ctccggattt gagcgtggcc acgtcggtca
    10741 cctttcggtc ggggtggcgg ccggccggga gtggccggcg gtctgttccg gctgccggac
    10801 ggcctcaggc ggctggctgg cgagccgctc gcgggtgcgc agccacacct cgcacgcgag
    10861 ggtgggatgc agctggtagc tctcggactc ggcggacagg gcgcgcaggc accacctgcg
    10921 cagctcgccc tcatccacca gcccgagccg ggcgagacgg gagtcctcgc acagggcgag
    10981 caggcccgcg cggtgcgcgc gcagtcccgc ctcctcctca taggtggcgt tggccttggt
    11041 gccacgggtc cgggagcttt cgggcacgac cccgcgcatg gcctccacga tgagcggttt
    11101 gtaccgccac ggggtgatgc gctcgtgcgg gcgtacggcc agggcggcct cgatgacccg
    11161 gacgtcgtag tagggcgcgg agaaggtgag gcccagggga gcggccatct ggccgatgtg
    11221 ccgggcccac tgggagatgc cctccatcgc ggccagctcg cggtgcgtcc cgtggcccgg
    11281 ggccagcggc tgggccgtgg ggagctcggc ccgcagcagt tcccgcgcgg ctgcgcgcgc
    11341 atcaccggtc gcccacggcg gcatccgcgg cggagcggcc cagtccagca gtggttcgtc
    11401 ggccgccggg gcgggagcgg tgagatcgtc cgctacgcgc gcgagccagg cccggtaggg
    11461 acgccggtcc cccagctgcc ggagcacggc ggagtaaggc caccggtact tcgcggtgta
    11521 accgcgcagg gcgcgcagcc cggtgcgcgg gtgggtgcgc agcagcgagt gcaggtgggc
    11581 gggggttccg gccaacagct cgtcgccgcc caggccgcac aggtgcagcc ccgagccccg
    11641 ggccgccgcg cgctcgatga tgcccatccg gcggttgcgg tccaccgtga tgatgctggg
    11701 ggtgtccagc acgtcctgga gggagtccac tccctcgaag gtcatggcca catcggcggc
    11761 gggcacgatg tggtgctcca ctccgggcag ttcctggacg gtgcgctcgg cccagtagac
    11821 gtcgtccccg gcctggtcgt gcagcgcggc ggtgtaggcg gccaccgtgg ccggtccgcg
    11881 ggccgcggtg cagcacaccg cggtggagtc caggccaccc aggtcggtgg tgaccaggga
    11941 acgcccgtgc gtgcggacct cgaccgctgc ccgcagttcc gtacgcagcc cctccgcccc
    12001 ctcggacagg ggtacctcag agctggggac gtgccaccag cgggaaaccg ctgtgcggct
    12061 cccgggctcc agcgtcagcc ggtgtccgct cggcagggcg tggacaccgt gccacagcgg
    12121 ggcgcccgtc agcggatgcg gtgctccgga ggtcagcagt tccacggcga cccgtcgttc
    12181 gtccagcccg gccccggtca gttccgcgat cacatccgcg cggtcggcgg cgaggacgca
    12241 gcggcccggg ccgggcaccg tcccgtagaa gacccggcgc agccccacgg cgctgccctg
    12301 cacccggacc cggcccgctt cgctgaccag caggtggaaa cttcccggcc acatccgccc
    12361 tgcctccccc cacgcggcgg ggccgcccgc cgctgcccgg gccgcctgct cgggagtgac
    12421 ccggtgttcc ccgatgacgg ctacccgggt atcgccgtgg ctgcccacgg cgataccctc
    12481 cggcgcccgg ctgcccacca cccaggggcg tccggaagca tgcgagaggt tgagcggggt
    12541 ggaacccaga ccggcggcaa gatgggcgcc ttcctcggtg tcgggcagca cggcgaacca
    12601 ggcggaaaga gctgggaagg atgtcgatcg ggacacgagg tatttcgcct tctctgcctg
    12661 tggcgggggt tgagaggaca gcaccgggtg gcggccggaa aagccgccac ccggtagaag
    12721 accggtcgtg aaccggaatt cacgactggt caggtccgcg acccttggcg ggtgcggacg
    12781 cccaggtcag agaagctgct tcccgccgag gacgtccttc ggcccgcggt ccttcaggcc
    12841 ggtgacctta cggaaaccgc cagccttggc aagagtgggt cgctcgtacg tcatggagct
    12901 ctccttttct cgggagaggg cagcacatca ctgcccaggg aatgcgctaa tccggtggcg
    12961 gtgaagcttt ccgccggtgc gcgccgctta ccgtagggaa tgattccgtc acgtcgcaag
    13021 ggcgcctgcc gtgaattttt tccggtcttg accggtacgc cgcgacgcgg ctatgtcaga
    13081 tgaaaaacgc aggtggtgcg ggctgcgaag ggtgaaggag agacgggtca acctggccca
    13141 ggacgtcgcg tatcactgtc aagggcagcg actggccatc tattctggcc gtaaacaaac
    13201 cctcgtgcct gatgcactta tcgtgaaccg ggctgtccga ggcgtgactg ctcaggtggt
    13261 tgattacgtg tgagcgggtg gcagccaggg gtttccgttg aagaggtc
//
