LOCUS       NZ_JAEVFI010000014     10399 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Streptomyces sp. JJ66 scaffold14_size84485, whole genome shotgun
            sequence.
ACCESSION   NZ_JAEVFI010000014
VERSION     NZ_JAEVFI010000014.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. JJ66
  ORGANISM  Streptomyces sp. JJ66
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 84485)
  AUTHORS   Jose,P.A. and Jha,B.
  TITLE     Draft genome of Streptomyces sp. JJ66
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 84485)
  AUTHORS   Jose,P.A. and Jha,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2021) Marine Biotechnology and Ecology Division,
            CSIR - Central Salt and Marine Chemicals Research Institute, G.B.
            Marg, Bhavnagar, Gujarat 364002, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-MAY-2017
            Assembly Method                   :: Velvet v. V 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 320.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019303495.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 03:51:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,446
            Genes (coding)                    :: 4,340
            CDSs (with protein)               :: 4,340
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 61
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 31 of 106
            Pseudo Genes (incomplete)         :: 82 of 106
            Pseudo Genes (internal stop)      :: 3 of 106
            Pseudo Genes (multiple problems)  :: 9 of 106
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:22:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 10213
            Orig. end                         :: 20612
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAEVFI010000014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(271..1236)
                     /locus_tag="JJV70_RS12135"
                     /old_locus_tag="JJV70_12135"
     CDS             complement(271..1236)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957696.1"
                     /locus_tag="JJV70_RS12135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_12135"
                     /product="SCO1860 family LAETG-anchored protein"
                     /protein_id="WP_308294793.1"
                     /transl_table=11
                     /translation="MKKSVLSLLVAAVLTGPAAAPAHAAGEDEQAGRAGTAAAAVLRAQ
                     LDVSLLDDTVQVPLATSWNAVSAPDADTGADRAGGTALTAQLDGVQGGEPFSVLRADVA
                     ESRAEVTEDAASAEVTLARAAVHVPGLPLLSLIEAEKVTAKVVCAVDEPTVAEAGALGT
                     VRVLGKRVTLSAAGPTVVTVPGVGEVNLELSQRETATGSAAASALRLAVTVNPLELNVA
                     KVAGSVTLAEVSCRTPVPGEQSAKPRPEQPEPSREPAPQGEEPQGEPGARVTRTQDEPQ
                     DEPHLAATGARSDTPYLLGGAAGLIGVGGALFLLRRRRQS"
     gene            complement(1423..2520)
                     /locus_tag="JJV70_RS12140"
                     /old_locus_tag="JJV70_12140"
     CDS             complement(1423..2520)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238577.1"
                     /locus_tag="JJV70_RS12140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_12140"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_219047876.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 2.4e-24, bitscore:
                     78.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDSAVLHITGRVLAGPEDVRDELWAVDGRVTYERPPGHRLRDAV
                     TVHGWALPGLVDAHCHVGLDAHGAVDEATSEKQALTDREAGTLLIRDAGSAADTRWVDE
                     REDLPRIIRAGRHIARTRRYIRNYAHEIEPEDLVAYVRREARRGDGWVKLVGDWIDRDA
                     GDLTACWPRDAVRAAIAAAHEEGARVTAHCFAEDSLRDLAEAGIDCVEHATGLTEDTIP
                     LFAERGIAIVPTLVNIATFPALADGGEKKFPRWAEHMRALHERRYDTVRAAYDAGIPVF
                     VGTDAGGSLAHGLVAQEVAELVRAGIPPLEALSATTWGAREWLGRPALAEGASADLVVY
                     DTDPRADVRVLAGPRRVVLRGRVVQ"
     misc_feature    complement(2681..2694)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.33"
                     /tool="antismash"
     misc_feature    2728..2737
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.1"
                     /tool="antismash"
     misc_feature    2750..2767
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.07"
                     /tool="antismash"
     misc_feature    2778..2795
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.31"
                     /tool="antismash"
     misc_feature    2790..2805
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(2936..2951)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.79"
                     /tool="antismash"
     gene            3012..3521
                     /gene="ectA"
                     /locus_tag="JJV70_RS12145"
                     /old_locus_tag="JJV70_12145"
     CDS             3012..3521
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358302.1"
                     /locus_tag="JJV70_RS12145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_12145"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_219047877.1"
                     /transl_table=11
                     /translation="MPEGLKLDTPRVDDGAAIWRIARDSQVLDLNSSYSYLLWCRDFAA
                     TSVVARDGNGEPVGFITGYVRPQDPRTLLIWQVAVDHGQRGRGLAGAMLDGLFARVSRE
                     LGTDRMETTITPDNAPSNKLFTSFAERHGAEVKRDVLFDGEVFPGDEHEPEVLYQIGPI
                     RTPVMA"
     gene            3639..4907
                     /gene="ectB"
                     /locus_tag="JJV70_RS12150"
                     /old_locus_tag="JJV70_12150"
     CDS             3639..4907
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-365). E-value: 7.6e-74.
                     Score: 240.6. Matches aSDomain:
                     nrpspksdomains_JJV70_RS12150_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="JJV70_RS12150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_12150"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_219047878.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.7e-80, bitscore:
                     261.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRGYCRGWPTIFDRAQGSHMYDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTVAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDAYFDGTTPDFLWFERLLEDSGSGLNQPAAVIVETVQGEGGINVARP
                     EWLRALAELCERHDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTISKSISGYGLPMS
                     LTMFKPELDIWEPGEHNGTFRGNNPAFVTATATLETYWADGQMEKQTLARGEQVEAALG
                     EIAKEHPEAVVEHRGRGLVWGMEMAEPAHAGQIAKRCFELGLLVETSGPDSEVVKMLPA
                     LTITPEELDEGLRILARAVRETV"
     aSDomain        3726..4733
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JJV70_RS12150_Aminotran_3.1"
                     /evalue="7.60E-74"
                     /label="JJV70_RS12150_Aminotran_3.1"
                     /locus_tag="JJV70_RS12150"
                     /protein_end="365"
                     /protein_start="29"
                     /score="240.6"
                     /tool="antismash"
                     /translation="DRAQGSHMYDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYLER
                     DGVTHGLDMSTVAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKGR
                     ESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDAYFDGTTPDFLWFERL
                     LEDSGSGLNQPAAVIVETVQGEGGINVARPEWLRALAELCERHDMLLIVDDIQMGCGRT
                     GAFFSFEEAGIVPDIVTISKSISGYGLPMSLTMFKPELDIWEPGEHNGTFRGNNPAFVT
                     ATATLETYWADGQMEKQTLARGEQVEAALGEIAKEHPEAVVEHRGRGLVWGMEMA"
     gene            5001..5399
                     /locus_tag="JJV70_RS12155"
                     /old_locus_tag="JJV70_12155"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947422.1"
                     /locus_tag="JJV70_RS12155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_12155"
                     /product="ectoine synthase"
                     /protein_id="WP_219047879.1"
                     /sec_met_domain="ectoine_synt (E-value: 8.8e-56, bitscore:
                     179.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLQEIENTDRDIRSASGTWRSKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKHWISPGTMYLLDGHEKHTLRPKTDFR
                     VICVFNPPVTGREDHDENGVYPLLTEDD"
     gene            5548..6450
                     /gene="thpD"
                     /locus_tag="JJV70_RS12160"
                     /old_locus_tag="JJV70_12160"
     CDS             5548..6450
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358305.1"
                     /locus_tag="JJV70_RS12160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_12160"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_219047880.1"
                     /sec_met_domain="PhyH (E-value: 1.4e-38, bitscore: 125.5,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERIITDLYPTRGATEAAVPRQDPVVWSDPDVTGPITAGHL
                     ADYERDGFLAVDQLITPDEVSVYRGELERLISDPEIRRDERSIVEPQSQEIRTVFEVHK
                     ISEVFAGLVRDPRLVERARQILGSDVYVHQSRVNVKPGFGARGFYWHSDFETWHAEDGL
                     PNMRTVSVSIALTRNYDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDE
                     ALTKFADAHGIRLFTGEAGSATWFDCNCLHGSGDNITPYPRSNVFIVFNSVENTAVEPF
                     AAPVRRPEFLGARDFTPVR"
     gene            complement(6603..7661)
                     /locus_tag="JJV70_RS12165"
                     /old_locus_tag="JJV70_12165"
     CDS             complement(6603..7661)
                     /NRPS_PKS="Domain: Aminotran_5 (42-341). E-value: 6.1e-22.
                     Score: 69.9. Matches aSDomain:
                     nrpspksdomains_JJV70_RS12165_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718911.1"
                     /locus_tag="JJV70_RS12165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_12165"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_219047881.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7.4e-18, bitscore:
                     56.8, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQPMSAEFSPTLTYLNTAAHGLFPARTTAVLHAAVREMAEAEMD
                     QVGYYERVEAARTAFAQLAGVPPTRVATGSAVAVHVGLIAQSLPEGTEVLVADGDFSSL
                     VNPFAVRRPGLVLRSVPLPELADAVRPSTGLVAVSAVQSADGRVADLAAVRAAAGAHGA
                     RVLVDTTQSTGWLTLDPALADFTVCGAYKWLLCPRGTSFLTVPEDGGGLRPLHAGWIAG
                     ENPWETCYGPVEELARSARRFDESPPFLPYLGAVPSLDLIAELGPEAIGKHNRGLAARF
                     RAGVRELGHRPVEADSAIVAVPGLGDAAEALAKAGIRLSTRVGNLRAAFHLFNTEDDVD
                     RALEVLGEHSRT"
     aSDomain        complement(6639..7535)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JJV70_RS12165_Aminotran_5.1"
                     /evalue="6.10E-22"
                     /label="JJV70_RS12165_Aminotran_5.1"
                     /locus_tag="JJV70_RS12165"
                     /protein_end="341"
                     /protein_start="42"
                     /score="69.9"
                     /tool="antismash"
                     /translation="EMDQVGYYERVEAARTAFAQLAGVPPTRVATGSAVAVHVGLIAQS
                     LPEGTEVLVADGDFSSLVNPFAVRRPGLVLRSVPLPELADAVRPSTGLVAVSAVQSADG
                     RVADLAAVRAAAGAHGARVLVDTTQSTGWLTLDPALADFTVCGAYKWLLCPRGTSFLTV
                     PEDGGGLRPLHAGWIAGENPWETCYGPVEELARSARRFDESPPFLPYLGAVPSLDLIAE
                     LGPEAIGKHNRGLAARFRAGVRELGHRPVEADSAIVAVPGLGDAAEALAKAGIRLSTRV
                     GNLRAAFHLFNTEDDVDR"
     misc_feature    7612..7625
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     misc_feature    7975..7995
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.58"
                     /tool="antismash"
     gene            7995..9215
                     /locus_tag="JJV70_RS12170"
                     /old_locus_tag="JJV70_12170"
     CDS             7995..9215
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1296: tellurium
                     resistance protein, TerD"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671960.1"
                     /locus_tag="JJV70_RS12170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JJV70_12170"
                     /product="TerD family protein"
                     /protein_id="WP_219047882.1"
                     /transl_table=11
                     /translation="MTPGSNLPLTAARVAVEVAAPVRLDVSGLLLAADGKVRSDADFVF
                     YNQPSGPGVTHSSSGSTDTITVDTAAIPAEIDKVVVTASLDTPGGTFAGTEPTGTVRDA
                     ASGAVLATFTPSQLGAETALVLVEVYRRGGTWKVRAVGQGYANGLAGIATDFGVTVEEP
                     APAPAATAPPAAPGPVSAPAAPAAAQPAAPAPGAQPVQPAQPAQPAAPAPGAGKINLDK
                     GRVSLQKNQSVSLVKDNQPLRSSLVMGLGWEPAFRGKSIDLDASVIAFDARRKKIDECY
                     FGKLAILRGAIQHSGDNLTGEGAGDDETIRVHLDGLPPEVTGLVFTVNSFTGQKFSEVA
                     KAYCRLLDERGQELVRFDLTDAEPRTAVLMCKFVRQFSGEWEMTALGQFLDGRTVRSLV
                     KPAGQAL"
ORIGIN
        1 acgcgacgcc ggaaccggcc gcgccgttcg tgctgatccg gcacccccgc gccgccgggg
       61 tacgcgagcg gctgcgcggg ctgggcttcg cggtgcggcg cggtgacacc ttccccggcc
      121 tgggcccgga gtggctgcgg ctggcggtgc gcgacccggc gacgagtgac cggttcgcca
      181 ccgccctgac ggcagccgga gtgcgctgag cacggcctgg cggacacgcc gggccgtgcc
      241 cctcggttgg ctgccgacgc gcgggccggt tcagctctgg cgacggcgcc ggagcaggaa
      301 cagggcgccg cccaccccga tgagacccgc ggcaccgccc agcaggtagg gcgtgtcaga
      361 ccgggcgccc gtggcggcca ggtgcggctc gtcctgcggt tcgtcctgcg tccgcgtcac
      421 ccgcgcaccg ggctcgccct ggggttcttc gccctgcggc gcgggctccc gcgacggctc
      481 gggctgctcc ggacgcggct tagcggactg ctcgccgggg acgggagtgc ggcagctgac
      541 ctcggccagc gtgacgctgc ccgccacctt cgccacgttc agctccagcg ggttgacggt
      601 cacggccagc cggagcgcgc tcgcggcggc gctgcccgtg gccgtctccc gctgggacag
      661 ctccaggttc acctccccga cgccgggcac cgtgacgacg gtcggtccgg cggcgctcag
      721 cgtgacccgc ttgccgagca ccctcaccgt gccgagcgcg cccgcctcgg ccacggtggg
      781 ctcgtccacc gcgcagacca ctttcgcggt gaccttctcc gcctcgatca gcgacagcag
      841 cggcagcccg gggacgtgca ccgccgcccg cgccagtgtg acctccgccg atgcggcgtc
      901 ctcggtgacc tcggccctgg actcggcgac gtccgcccgc agcacgctga agggctcgcc
      961 gccctgcacg ccgtcgagct gggcggtcag cgcggtgccg cccgcgcggt cggcaccggt
     1021 gtcggcgtcc ggtgcggaga cggcgttcca actggtggcc agcgggacct ggacggtgtc
     1081 gtccagtagg gagacgtcga gctgggcgcg cagcaccgcg gcggccgcgg tgcccgcgcg
     1141 tccggcctgc tcgtcctcgc cggcggcgtg ggcgggggcc gccgccgggc cggtgagtac
     1201 ggcggcgacg agcaggctga ggacagactt cttcacggtt gggaactccc acagtggttc
     1261 ggtgtcaccg gcgcggcctg acgggggaca tcactcgtgc cggtgacggg gaccccgaca
     1321 tcgttgacgc caccgccgtc acccgtccgg gttctccgac gggttcaccc ggacggggcc
     1381 tcgccgaacg cgcgcacgag gggcggggag ctgaccgtgc cgctactgca ccacccgccc
     1441 ccgcagcacc acccgacgcg gtccggccag cacccggacg tcggcacgcg ggtcggtgtc
     1501 gtagaccacc aggtccgcgg acgcgccctc ggcgagcgcc gggcgcccca gccactcccg
     1561 cgcgccccac gtcgtcgccg acagcgcctc cagcgggggg atgcccgcgc gcaccagttc
     1621 ggccacctcc tgcgcgacca gcccgtgggc cagcgacccg cccgcgtcgg tgccgacgaa
     1681 gacggggatg cccgcgtcat aggcggcgcg caccgtgtcg tagcggcgct cgtgcagggc
     1741 gcgcatgtgc tcggcccagc gcgggaactt cttctcaccg ccgtcggcga gtgccgggaa
     1801 cgtggcgatg ttgaccagcg tcggcacgat cgcgatgccg cgctcggcga acagcgggat
     1861 cgtgtcttcg gtgaggccgg tggcgtgctc cacgcagtcg atgcccgcct cggccaggtc
     1921 ccgcagtgag tcctccgcga agcagtgcgc ggtgacgcgg gcgccctcct cgtgggcggc
     1981 ggcgatggcc gcgcgcacgg cgtcccgggg ccagcaggcg gtcaggtcac cggcgtcgcg
     2041 gtcgatccag tcccccacca gcttcaccca gccgtcgccg cgccgcgcct cccggcgcac
     2101 gtaggcgacc agatcctccg gctcgatctc gtgcgcgtag ttgcggatgt agcggcgggt
     2161 ccgggcgatg tggcggcccg cgcggatgat ccggggcagg tcctcgcgct cgtccaccca
     2221 gcgggtgtcg gcggccgaac cggcgtcccg gatcagcagc gtgcccgcct cgcggtcggt
     2281 gagcgcctgc ttctcgctcg tcgcctcgtc cacggcgccg tgggcgtcca gaccaacgtg
     2341 gcagtgggcg tccacgagcc cgggcagggc ccagccgtgc accgtgacgg cgtcacgcag
     2401 gcggtggcct ggcggccgct cgtaggtgac gcggccatcc accgcccaca gctcgtcccg
     2461 tacgtcctcc gggccggcca gcacccgccc cgtgatgtgc aacaccgcac tgtccttcat
     2521 atctccgcac tgtaagcctc atgggggcgg agccgtgccg cgcgggggcc gggagggggg
     2581 cgaggggtgg gggattgtgg cggggatcaa ttcgtgcaag ggtgcggtga ctttacggag
     2641 cgactccgca gagtggaagg aatgcaccac tcagccattc tgcatagcca tccgtatgct
     2701 tcttcccggt tttctccaag ccaaacctac ttttttaccg aaccctcggg agcccgcagt
     2761 gcggacaggc gtgacggtcg ttatgcacct gtgacggatt ccacaggagt ggcgttacgc
     2821 cccggcatat ccgatatgac caggcaatcc gtcgccgtta ccagcttcag gcgcgcacgc
     2881 gcgcgataac atatgcgcgt gccatgcgta accccttccc acgatgcaac ttcgattttt
     2941 cctgggtaaa ttgattcgac atgacccccg cacaagctga ccgtgcacgt gcccgaagcg
     3001 aattcatgga aatgcccgag ggactcaagc tcgacacacc acgggtggac gacggagccg
     3061 ccatctggcg gatcgcccgc gactcccagg tgctcgacct gaactcctcg tacagctacc
     3121 tcctgtggtg ccgcgacttc gccgccacct ccgtagtggc tcgcgacggg aacggcgaac
     3181 cggtcggctt catcaccggc tacgtccggc cccaggaccc ccgcaccctg ctgatctggc
     3241 aggtcgccgt ggaccacggc cagcgcgggc gcggactggc cggagcgatg ctggacgggc
     3301 tcttcgcccg ggtgtcccgg gagctgggga cggaccgcat ggagacgacg atcaccccgg
     3361 acaacgcccc ctccaacaag ctgttcacct ccttcgccga gcggcacggg gccgaggtca
     3421 agcgcgacgt gctcttcgac ggcgaggtct tccccggcga cgagcacgag cccgaggtgc
     3481 tgtaccagat cggcccgatc cgtacccccg tcatggcctg acgcgcccgc ggcccgcccc
     3541 gcccggcggt ctccgcgacg tccggccccg gggaccggga ccggtgccag gccccaccag
     3601 acttcacgct tttcacgact cactcccagg agccatccgt gaccatcacc cagcctgatc
     3661 tgagcgtctt cgagactctg gagtccgagg tccgcggtta ctgccgcggc tggcccacga
     3721 tcttcgaccg ggcccagggc agccacatgt acgacgagga cggccacacc tacctggact
     3781 tcttcgccgg cgccggctcg ctcaactacg ggcacaacaa cccggtcctc aaacgcgcgc
     3841 tgatcgacta cctcgaacgc gacggggtga cccacgggct ggacatgtcc acggtcgcca
     3901 agcgggcgtt cctggagacg ttccagaacg tcatcctgcg cccgcgtgac ctgccctaca
     3961 aggtcatgtt ccccggcccg acgggcacca acgcggtcga ggccgcgctg aagctggccc
     4021 gcaaggtcaa ggggcgcgag tcggtcgtgt ccttcaccaa cgccttccac ggcatgtcgc
     4081 tgggctcact cgccgtcacc ggcaacgcct tcaagcgggc cggcgcgggc atcccgctgg
     4141 tgcacggcac cccgatgccg ttcgacgcct acttcgacgg gacgaccccg gacttcctgt
     4201 ggttcgagcg gctgctggag gactccggtt ccggcctgaa ccagcccgct gccgtgatcg
     4261 tcgagacggt gcagggtgag ggcggcatca acgtcgcccg tcccgagtgg ctgcgcgccc
     4321 tggcggagct gtgcgagcgg cacgacatgc tgctcatcgt ggacgacatc cagatgggct
     4381 gcggccgcac cggtgccttc ttctccttcg aggaggcggg catcgtcccg gacatcgtca
     4441 ccatctcgaa gtcgatcagc ggctacggtc tgccgatgtc gctgacgatg ttcaagccgg
     4501 agctggacat ctgggagccg ggcgagcaca acggcacctt ccgggggaac aacccggcct
     4561 tcgtcaccgc caccgccacg ctggagacgt actgggcgga cggccagatg gagaagcaga
     4621 ccctggcccg tggcgagcag gtcgaggcgg cgctgggcga gatcgccaag gagcacccgg
     4681 aggccgtcgt cgagcatcgc ggccgtggcc tggtgtgggg catggagatg gccgagcccg
     4741 cgcacgccgg gcagatcgcc aagcgctgct tcgagctggg tctgctggtg gagacctctg
     4801 gcccggacag cgaggtcgtc aagatgctgc ccgcgctgac catcacgccc gaggagcttg
     4861 acgagggact gcgcatcctg gcccgtgccg tccgcgagac tgtctgacgc accgcccttc
     4921 cggcccgccc cgtatccggg gcgggccgcc ccgtagctgc ccgaccgggc agccgaacga
     4981 tcagaaaggc accacgagaa gtgatcgtcc gttccctcca ggagatcgag aacaccgacc
     5041 gcgacatccg ctccgcgtcg ggcacctggc gcagcaagcg catcgtgctg gccaaggaga
     5101 aggtcggctt ctcgctgcac gagacggtgc tgtacgcggg caccgagacc tcgatgtggt
     5161 acgcgaacca catcgaggcc gtgctgtgcg tcgaaggtga agccgagctc accaacgacg
     5221 agaccggtga gaagcactgg atcagccccg ggacgatgta cctgctcgac gggcacgaga
     5281 agcacacact gcgcccgaag acggacttcc gggtgatctg cgtcttcaac cccccggtga
     5341 ccgggcgcga ggaccacgac gagaacggcg tctacccgct cctcaccgag gacgactgag
     5401 ccacggacgc ggggtgtggt gcgagaacca ccctccgcgt ctttggaatg tttcctgtgg
     5461 ggaacacgtg accgcgtggg ggcgactccc caggagacga ctgacacgaa ggacaggacc
     5521 gcgcgagagg agaggaagga aactcccatg accaccgcac ccgagcgcat catcaccgac
     5581 ctttacccga cccggggggc gaccgaagca gccgtcccgc gtcaggaccc ggtggtgtgg
     5641 tccgacccgg acgtcaccgg cccgatcacg gccgggcacc tggcggacta cgagcgtgac
     5701 gggttcctcg cagtagacca gctgatcacc ccggacgagg tgtcggtcta ccgcggagaa
     5761 ctggaaaggc tcatctcgga cccggagatc cgccgcgacg agcgctcgat cgtcgagccg
     5821 cagtcgcagg agatccggac cgtgttcgag gtgcacaaga tcagtgaggt gttcgccggg
     5881 ctggtgcgcg acccgcggct cgtcgagcgg gcgcggcaga tcctcggctc ggacgtctac
     5941 gtccaccagt cccgggtcaa cgtgaagccg gggttcggcg cccgtggttt ctactggcac
     6001 tcggacttcg agacgtggca cgcggaggac ggtctgccga acatgcggac cgtttcggtg
     6061 tccatcgcgc tgaccaggaa ctacgacacc aacggcggcc tgatgatcat gccggggtcg
     6121 cacaagacct tcctcggctg cgcgggcgag acgccgaagg acaactacaa gaagtcgctg
     6181 cagatgcagg acgccggtac gccctcggac gaggcgctga ccaagttcgc cgacgcccac
     6241 ggcatcagac tgttcaccgg cgaggccggt tcggccacgt ggttcgactg caactgcctg
     6301 cacggttccg gcgacaacat cacgccctac ccgcgcagca acgtgttcat cgtcttcaac
     6361 agcgtggaga acaccgcggt ggagcccttc gccgcgcccg tgcggcggcc ggagttcctc
     6421 ggcgcccgtg acttcacccc ggtgaggtaa cccgcgtcgg tcccggtccg gcgtgcgggc
     6481 cgggacaggt aaccaggagg ccccgggccc gggtgccgcc ctccgcgggg cggcccccag
     6541 gccgggggcg tttctgtgtg cggacaccgg tgcgcccgca ctcggcgccg gcgccgtgac
     6601 cgttaggtgc ggctgtgctc gcccagcacc tccagcgcgc ggtccacgtc gtcctcggtg
     6661 ttgaacaggt ggaacgcggc gcgcaggttg cccacgcggg tggagaggcg gattccggcc
     6721 ttggccagcg cctcggccgc gtcgcccagg ccgggcaccg cgacgatggc cgagtccgcc
     6781 tccaccgggc ggtgccccag ctcccgcacc ccagcccgga accgggccgc caggccccgg
     6841 ttgtgcttcc cgatggcctc ggggcccagc tcggcgatca ggtcgaggga gggcaccgcg
     6901 cccaggtagg gcaggaacgg cggcgactcg tcgaagcggc gggcgctgcg ggccagctcc
     6961 tcgaccggcc cgtagcacgt ctcccacggg ttctcccccg cgatccagcc agcgtgcagg
     7021 ggccgcagcc cgccgccgtc ctccggcacc gtcaggaacg acgtgccacg ggggcagagc
     7081 agccacttgt aggcgccgca gacggtgaag tcggccagcg cggggtccag cgtcagccag
     7141 ccggtggact gcgtggtgtc caccaggacg cgggcgccgt gggcgccggc ggcggcgcgg
     7201 acggcggcca ggtcggcgac gcggccgtcg gcggactgca ccgcgctgac cgccacgagc
     7261 cccgtgctgg gccgcaccgc gtcggccagc tccggcagtg gcaccgaacg gaggacgagt
     7321 ccggggcggc gcacggcgaa cgggttgacc agcgagctga agtccccgtc ggccaccagc
     7381 acctcggtgc cctcgggcag cgactgggcg atcaggccga catggacggc cacggccgaa
     7441 ccggtggcca cccgcgtcgg cggcacgccc gccagctgcg cgaacgcggt ccgggcggcc
     7501 tcgacccgct cgtagtagcc gacctggtcc atctcggcct ccgccatctc ccgcaccgcc
     7561 gcgtgcagga cggcggtggt gcgggccggg aagagaccat gggcggcggt gttgaggtag
     7621 gtcaacgtgg ggctgaactc ggcactcatc ggttgcgaca tgccaccgac tctctgtcac
     7681 cgggccagag ttgtccatgg ccaccggaca gcgcgtggcc cggagcgggc cgcgcgcggg
     7741 tgacatcccg tggcatgcgc tgcgtctttc cgtgccacag gtcacccgga cgccgcaacg
     7801 gggattctga gctgggccga tggcgagcga cgcgggcctg agcgtcggca ccccgccgcc
     7861 cgccggactg ccgggctcgt gggcgcggtg ggctgcccgc gtccggcggg cagccgtcgc
     7921 ctgggcctgg gccggtcgct gcggcggtgt ggaaccgtgc tgctccgccg gtcgttaacg
     7981 acggcatgac cgctatgact cccggctcga acctgcccct gaccgcggcc cgggtggccg
     8041 tcgaggtcgc cgctccggtg cggctggacg tctcgggcct gctgctcgcc gccgacggca
     8101 aggtgcgctc cgacgccgac ttcgtgttct acaaccagcc ctccggcccc ggcgtgacac
     8161 acagcagcag cggcagcacc gacaccatca ctgtggacac ggccgcgatc ccggccgaga
     8221 tcgacaaggt cgtagtaacg gcgagcctgg acactccggg cggtacgttt gcggggaccg
     8281 agcccaccgg caccgtgcgg gacgccgcga gcggcgcggt gctggccacg ttcaccccgt
     8341 cacagctcgg cgcggagacg gcactggtgc tggtggaggt ctaccggcgg ggcggcacgt
     8401 ggaaggtgcg ggcggtcggc cagggttacg ccaatgggct ggcgggtatc gccacggact
     8461 tcggcgtcac cgtcgaggag cccgctccgg ctccggcagc caccgcgccg cccgctgccc
     8521 cggggcctgt ctccgctccg gcagccccag cggccgcgca gcctgccgct ccggctcccg
     8581 gcgcacagcc cgtgcagccc gcacagcccg cacagcccgc cgctccggct ccgggcgcgg
     8641 ggaagatcaa tctggacaag gggcgcgtca gcctgcagaa gaaccagtcg gtctccctgg
     8701 tcaaggacaa ccagccgttg cgctcctcgc tggtcatggg cctgggctgg gaaccggcgt
     8761 tccgtggcaa gagcatcgac ctggacgcct ccgtcatcgc cttcgacgcc cggcgcaaga
     8821 agatcgacga gtgctacttc ggcaagctgg ccatcctgcg gggtgccatc cagcactcgg
     8881 gcgacaacct caccggtgag ggcgcgggcg acgatgagac catccgcgtc cacctcgacg
     8941 gtctgccgcc ggaggtgacg ggcctggtgt tcaccgtcaa ctccttcacc gggcagaagt
     9001 tcagcgaggt cgccaaggcg tactgccgtc tgctggacga acgcggccag gagctggtgc
     9061 ggttcgacct gacggacgcc gagccgcgca ccgccgtcct catgtgcaag ttcgtccggc
     9121 agttctcggg cgagtgggag atgacggcgc tcggccagtt cctggacggc cgcacggtgc
     9181 gcagcctggt caagcccgca ggccaggccc tgtaacaggc gcccaccgga cggctggagc
     9241 gcggcacggg cacaaggtgg cggggacgca cgcagtaggc gccgtgcgcc cccgcgcagc
     9301 tctcgtggtg tggaaccagc gatggcccgc gcgccggtga cggctccagc cgaagggtgg
     9361 cgcccaccgg cccgccccgg ccggagcggg ccgacgcagg cgtccgctgc cctggcccgt
     9421 gatcgtcacg cggtgcggga gcgggccttg tacgccgcct tacgggccgc cttggccgcc
     9481 gcgcggtcag ggtgcagacg ggccatcgcc tccagcacga gcggcgtggc ggggtgctcc
     9541 acgcgccacg ccttgtcgaa gaacccgctg tgctgggcca ccagaccggc catcagctcg
     9601 ctcagctccg gagcggcggc ggccgggtcg tccgggtcca gctgggcggc cagcgtgtcc
     9661 acggtgagcc agaagatcag ctcttcgccg ggcggcggga catcggcggc gccgtgctcg
     9721 gcgagccaca cccgggccag gccgcccagt tcggcgtcat cgagcacctc ccgcagggcg
     9781 ggttcggcct cggctccggc cgccgcgagc ccctgctggc acagcagccg gcgtcgcggg
     9841 gccagcgcgt cccggccccg ggcggcgtcc agcagcgccc gggcggcgtc cagcgcgggg
     9901 cgccgggcca gccactgttc cagctccacc tgggccagcg cctccgggta ggccgtcacg
     9961 gcggcgagca gggcgggggc gtcggcgtcc accaggtcac ccacggctgg tgcccgcacg
    10021 cccgcgtcga gcatgctggc gcgctccccg tacaggccca gcggggtgaa ccggaccatc
    10081 ccgtaccggc tcagttcctc ctccggcagc tccgaggcgg tggacagcgc ggtgggcggc
    10141 tcctctccct cgtgcggttc ggcctcctgc aacagcgcct cgtccaccgg gcggtactcc
    10201 accccgccgg acggctccag cacgcgcaac tgcgcgtcga gccgcatcat cacctcagag
    10261 acgtcctcca gcacggcgtc ggtggggtgg tccatgtcgt cggggacgac catcgaggcg
    10321 gccagcacgg gcagcggcac catcgcgccg tcggcgacgg ccgggtcctc ggcggtgagg
    10381 aggtagaggt tggcgagcg
//
