LOCUS       NZ_CP079114            10798 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. WY228 chromosome.
ACCESSION   NZ_CP079114
VERSION     NZ_CP079114.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WY228
  ORGANISM  Streptomyces sp. WY228
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8286721)
  AUTHORS   Qin,S.
  TITLE     Volatile organic compounds produced by endophyte Streptomyces sp.
            WY228 mediate plant growth and salt stress tolerance by activating
            hormonal signaling and anthocyanin biosynthesis,
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8286721)
  AUTHORS   Qin,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2021) Key lab of biotechnology of medicinal
            plants, Jiangsu Normal University, No. 101 Shanghai Road, Xuzhou
            221116, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019287315.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 06:02:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,303
            CDSs (total)                      :: 7,213
            Genes (coding)                    :: 7,059
            CDSs (with protein)               :: 7,059
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 83 of 155
            Pseudo Genes (incomplete)         :: 99 of 155
            Pseudo Genes (internal stop)      :: 10 of 155
            Pseudo Genes (multiple problems)  :: 36 of 155
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:35:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7657542
            Orig. end                         :: 7668340
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079114.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="32"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5798)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    149..164
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.72"
                     /tool="antismash"
     misc_feature    complement(162..176)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.76"
                     /tool="antismash"
     misc_feature    complement(275..294)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.72"
                     /tool="antismash"
     misc_feature    complement(311..324)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.45"
                     /tool="antismash"
     gene            complement(326..1170)
                     /locus_tag="KV381_RS33340"
                     /old_locus_tag="KV381_33340"
                     /pseudo=""
     CDS             complement(326..1170)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /locus_tag="KV381_RS33340"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_33340"
                     /product="IS5 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDHGVVIRRHELSDAEWEFVRPLLPVSLRGRKRLDDRRVLNGIVW
                     KFRTGTAWRDVPERYGPWATLHTRFRRWAADGTFERMLRAAQARADAAGDIEWLVSVDS
                     TVVRAHQHAAGARKGGSATRPSAGPGAV"
     misc_feature    1172..1187
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.02"
                     /tool="antismash"
     misc_feature    1175..1188
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.94"
                     /tool="antismash"
     misc_feature    complement(1241..1255)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.58"
                     /tool="antismash"
     misc_feature    1242..1255
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.83"
                     /tool="antismash"
     gene            1341..1766
                     /gene="panD"
                     /locus_tag="KV381_RS33345"
                     /old_locus_tag="KV381_33345"
     CDS             1341..1766
                     /EC_number="4.1.1.11"
                     /GO_function="GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="panD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964661.1"
                     /locus_tag="KV381_RS33345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_33345"
                     /product="aspartate 1-decarboxylase"
                     /protein_id="WP_030622987.1"
                     /transl_table=11
                     /translation="MMRTMFKSKIHRATVTQADLHYVGSVTIDAALMEAADLLPGELVH
                     IVDIDNGARLETYVIEGERGSGVIGINGAAAHLVHPGDLVILISYAQVDDAEARALVPR
                     VVHVDADNRIVALGDDASAPVPGSRTERSPQAVVPGG"
     misc_feature    1343..1358
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.02"
                     /tool="antismash"
     misc_feature    1345..1359
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.7"
                     /tool="antismash"
     gene            1833..2186
                     /locus_tag="KV381_RS33350"
                     /old_locus_tag="KV381_33350"
     CDS             1833..2186
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955645.1"
                     /locus_tag="KV381_RS33350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_33350"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_030622985.1"
                     /transl_table=11
                     /translation="MAENAAVEIRDDREHGNLVAYEDGTSAGTIAYFVMDPAPGALVAV
                     HTVVRPEHEGKGIAGALVREFYAMAAREGVPVVPLCPYAAKWAQRHPDEAPAAPGELVR
                     EAERQLRHHPELF"
     gene            complement(2214..2980)
                     /locus_tag="KV381_RS33355"
                     /old_locus_tag="KV381_33355"
                     /pseudo=""
     CDS             complement(2214..2980)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955646.1"
                     /locus_tag="KV381_RS33355"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_33355"
                     /product="PIG-L deacetylase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPLPSILAVHARTTSPCSAEAYWPGTRRPAPARRWSPRPGRGAPT
                     GPPSWPGPWTPWARALRACSATRTPGYRSRPRAGPGSSTLRWTKPWRRWSATSGTSGRR
                     S"
     gene            3195..3401
                     /locus_tag="KV381_RS33360"
                     /old_locus_tag="KV381_33360"
     CDS             3195..3401
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KV381_RS33360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KV381_33360"
                     /product="hypothetical protein"
                     /protein_id="WP_078611318.1"
                     /transl_table=11
                     /translation="MTHPYPDPVPPSPTPPPVPGPPTPVPGPPPVPPGPGPVPPGPAPD
                     PIPQPPGPDPVPDPEPEPQPAGT"
     gene            complement(3592..4968)
                     /locus_tag="KV381_RS33365"
                     /old_locus_tag="KV381_33365"
     CDS             complement(3592..4968)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955647.1"
                     /locus_tag="KV381_RS33365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_33365"
                     /product="diaminopimelate decarboxylase"
                     /protein_id="WP_218786973.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 4.7e-37, bitscore:
                     126.4, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 1.5e-07,
                     bitscore: 30.3, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQTTSAPHHEDGTAPPAARFDAVVEAAVRQGLLGEGGPVAGFID
                     TEGVRASVGALHDAFAATPGVLHTFAAKACSLIPVLRLLADCGMGCEVASPGELRLALE
                     AGFPASRIVLDSPAKTREEIRLALALGVAVNADSLDELRRVDALRNPGTASVLGLRVNP
                     QVGGGAIGAMSTATDTSKFGVALRDAGAREGIVTAFRERPWLTRLHAHVGSQGCPLELI
                     AAGVAQTYELAEEINATLGVRQVTSLDIGGGLPVNFADETVHPTYADHVAALTAAAPGL
                     FSGRYALVTEFGRSLLAKNGFIGARVEYTKDAGGRRIALTHAGAQTATRTVLMPEAWPL
                     RIGAFGPDGTPKGGPVLAQDIAGPCCFAGDVVAYGRELPELAQDDVVVLYDTGAYYFST
                     PWAYNSLPRPAVYGFRVAAGAGRDRAAVTFATVRDAQSMDAIAAESGLAHADALVERSD
                     "
     gene            complement(5001..5798)
                     /locus_tag="KV381_RS33370"
                     /old_locus_tag="KV381_33370"
     CDS             complement(5001..5798)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955648.1"
                     /locus_tag="KV381_RS33370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_33370"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_030089076.1"
                     /sec_met_domain="Linocin_M18 (E-value: 4.9e-88, bitscore:
                     293.2, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPVTPSAWDEIEEEARRTFRRHVAGRRVVDVSDPDGP
                     TLSAVGDGHLRDIDPPTPDVVARARTSMPVIEWRVPFTVTRQAVDDVERGSADSDWQPV
                     KDAARTCAFAEDMAIIDGYGAAGITGLRDGSSHEPLPLPADARDYPVAVSQAVTRLRLA
                     GVDGPYRLLLGADAFTEAAETSDHGYPVKTHLSRLVDDEILWAPAVKGGVLLSTRGGDF
                     ELCLGQDLSIGYADHDATSIHLYFQQAFTFRMLTPEAVVGLIA"
     gene            complement(5798..6904)
                     /locus_tag="KV381_RS33375"
                     /old_locus_tag="KV381_33375"
     CDS             complement(5798..6904)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456253.1"
                     /locus_tag="KV381_RS33375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_33375"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_218786974.1"
                     /transl_table=11
                     /translation="MPDAVPDPVSVQPVVAPLTSAALFLVGTIEPGGEGAVRDVLPDLA
                     AVARSLGFRYPDSGLACVAGFGSSAWDRLFAGPRPAHLHPFPELRGPLHHAPATPGDVL
                     LHIRAERMDVCYAWAAQLLDKLGGALRIVDETHGFRYLDHRDLLGFVDGTENPVGDDAR
                     SAALVGADDDPDFEGGSYVVVQKYLHDLTSWNALSVEEQERVIGRTKLDDVEFPDEDKP
                     ADSHLALNTITDPDGTERDILRANMPFGSFGKGEFGTYFIGYAADPDVTERMLRNMFLG
                     DPPGTHDRILDFSTAVTGSLFFAPRADFLDAPPPLPAPARSGDRPEPVPVPVLQEPGPA
                     PASARQEPVAAGPDHGSLRIGSLQESAQ"
     gene            7175..7591
                     /locus_tag="KV381_RS33380"
                     /old_locus_tag="KV381_33380"
     CDS             7175..7591
                     /GO_function="GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013625.5"
                     /locus_tag="KV381_RS33380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_33380"
                     /product="peptidoglycan-binding protein"
                     /protein_id="WP_218786975.1"
                     /transl_table=11
                     /translation="MALTAPRRRTAIVFASLVLLGGTAAGGVAAAAETGAAAAAAYPCD
                     VKMSAAGRLSAGYYDGTTVVPSTSQVTAAGKEAQCLLWYQGHNPGTIDGIFGRNSQAAA
                     KKFQKGVNYACRTTLAEDGKVGQQTWPYLRRLYC"
     gene            complement(7622..9061)
                     /gene="gndA"
                     /locus_tag="KV381_RS33385"
                     /old_locus_tag="KV381_33385"
     CDS             complement(7622..9061)
                     /EC_number="1.1.1.44"
                     /GO_function="GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_process="GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="gndA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100: 3-hydroxyisobutyrate dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006141148.1"
                     /locus_tag="KV381_RS33385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_33385"
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="WP_030089070.1"
                     /transl_table=11
                     /translation="MSGTAQIGVTGLAVMGRNLARNFARNGFTVAVHNRTTSRTDALVE
                     EFGDEGTFVAAHTPEEFVAALERPRRLVIMVKAGDPTDAVIQEFAPLLEEGDVIIDGGN
                     AHFEDTRRREKELRERGIHFVGVGISGGEEGALNGPSIMPGGSAESYASLGPMLEKIAA
                     QAKDGTPCTTHIGPDGAGHFVKMVHNGIEYADMQLIAEAYDLLRAVAGYSPAQIADVFR
                     TWNTGRLDSYLIEITAEVLAHTDAATGKPFVDIVQDRAEQKGTGRWTVQIALDLGVPVS
                     GIAEAVFARSLSGHADLREAARGLPGPTPKPLDGAAAAAFADQVEQALYASKVVSYTQG
                     FHQIRAGGETYDWDIDLGSVAAIWRAGCIIRAAFLDRIRAAYDSRPDLVSLLSDKEFGQ
                     EIGAAQDDWRAVVAEAVRQGVPTPGFAAALSYYDGLRADRLPAALTQGQRDFFGAHTYR
                     RTDREGSFHTLWGGDRTEVES"
     gene            complement(9217..9759)
                     /locus_tag="KV381_RS33390"
                     /old_locus_tag="KV381_33390"
     CDS             complement(9217..9759)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955650.1"
                     /locus_tag="KV381_RS33390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_33390"
                     /product="DUF5709 domain-containing protein"
                     /protein_id="WP_030089068.1"
                     /transl_table=11
                     /translation="MSDTDWGDDVYQPQEPEASDPDEQLGAEDTLMDPSGTGDPLDEGY
                     SPPDRPWVVDGLGTTADERNTGESLETRLTREVPEPDGSAGDGVGDLVDGDGEPWDDEV
                     GVARAGRLTQQADGRVPATLTASDVGIDGAAASAEEAAMHVIPEPGDDDALLEAGPPPA
                     APGSGPAGGGTAAPPPS"
ORIGIN
        1 cccgccatga cagcctccgc tctcgcgccc ttcgctgcag ccactctcgc ctcaattctg
       61 ggcggctacc gggagttgtg cggagtgctt gccggcctcg cggcggcgtc cgcgatcgtg
      121 gccacggcga catccgtacg aggaagggaa tgatcctgat catcgaggcc gacgatctcg
      181 gcacagtgtc ggtgacgtcc gtatgcacgg gcttccgcat gcccgatggg ccatcaggca
      241 agtcagcatt cggtcagcat gagtccccgc gcgatccggg gcggtcgtcc caacaggcgc
      301 cgctactagg ttctgtcgtc aaatgtcacg cccacatcag gagtgaggcg agggtgacgg
      361 ctgcttcgta ggactggacg gtcttgtcgt agcgtgtggc gatgccgcgc cattgcttca
      421 gacggttgaa gcaccgctcc acgacgttgc ggtgcttgta ggcctcgcgg tcgaaggccg
      481 gcgggcggcc accgcggctg ccccgccggg cccggttgcc gatctggtcg gcccgctcgg
      541 gaatcgtgtg gggatgccgc ggcggcggag ccaggtgcgg atcgccttga gctgtagccc
      601 ttgtcgccca ggacgtgatc gggccggacg cggggacggc cggggccgat ccgggcaccc
      661 tgatcgcgtc catcacggcg gtgaactggg tgcagtcgtt ggtgttgccg cccgtcacga
      721 cgaagccgag cggacggccc cggccgtcgc aggccaggtg caccttgctg gtcagaccgc
      781 ccctggaccg gccgagggcc gggtcgcaga gccccctttt cgagccccgg cagcatgctg
      841 gtgggcccgg acgaccgtgg aatcgaccga caccagccac tcgatgtcgc ccgccgcgtc
      901 ggccctcgcc tgcgcggccc gcagcatccg ttcgaacgtc ccgtccgccg cccacctgcg
      961 aaaacgcgta tgcagcgtgg cccacggccc gtagcgctcg ggcacatccc gccaggccgt
     1021 cccggtccgg aacttccaca cgatcccgtt caggaccctg cggtcatcca gccgcttccg
     1081 cccccgcaac gacacgggca gcaacggccg gacgaactcc cactcagcat cagacagttc
     1141 atgacgacgt atcacgacac catgatccac caacgatgat catttgaaga caccgcctaa
     1201 gggacacccc agacaggaag ctgtccagct cgaccggagc gattttccgc acaaacatcg
     1261 ttcggcgcgt cccggtgtac agtcgggacc gcccttgacc tgcacaaagc aggcagggag
     1321 cctagcccag gagtaccgca gtgatgcgaa ccatgttcaa gtccaagatc caccgggcca
     1381 ccgtcaccca ggccgacctg cactatgtcg gttccgtgac gatcgacgcc gccctgatgg
     1441 aggccgccga cctgctgccc ggcgagttgg tccatatcgt tgacatcgac aacggcgccc
     1501 gcttggagac gtacgtcatc gagggcgagc gcggctccgg tgtcatcggg atcaacggcg
     1561 cggccgcaca cctcgtgcac cccggtgacc tggtcatcct gatcagctac gcgcaggtcg
     1621 acgacgccga ggcccgtgca ctcgtgccgc gggtcgtgca cgtggacgcg gacaaccgga
     1681 tcgtggcact cggtgacgac gcctcggcgc cggttcccgg gagccgtacg gagcggagcc
     1741 cgcaggccgt cgtcccgggc ggctgaggcc gtcgtccgcg cttccgtgac gagcacggtg
     1801 gtgcgcggtc cacgaacgag agggcaggaa ccatggcgga gaacgcggca gtggagatca
     1861 gggacgaccg ggagcacggc aacctcgtgg cgtacgagga cggcacgtcg gccgggacca
     1921 tcgcgtactt cgtcatggac ccggctcccg gcgcgctcgt ggccgtgcac accgtggtcc
     1981 ggcccgaaca cgagggcaag ggcatcgcgg gcgcgctcgt gagggagttc tacgccatgg
     2041 ccgcccgtga gggcgtgccc gtcgtgccgc tgtgcccgta cgcggcgaag tgggcgcagc
     2101 gccaccccga cgaggcgccc gcggcgccgg gcgagctggt gcgggaggcg gagcggcagc
     2161 tcaggcacca tcctgagctg ttctgagccc tccgtccgca tcgcacgcgc ccgtcaggcg
     2221 ctcagctccg tggcggaccc gctccggggc gtgaggtcct ggcggatgta ccactcggtg
     2281 cccagcacct cccgctgaac cgccggaggc agagcggcga ttcggcccgg tgccgcccct
     2341 cgttccacct cgctgcggtg ggcgaggatc gcggcgagct tctgcgggag ccaggggccg
     2401 acgtcgaccg tcgtggtgat ccacgcgtcc tccgagcagt acagcgcgcc ggccggggtg
     2461 cccgcccggg ccagccgtcc acccaccgga acggccgccg agcggggatg cgtggcgagg
     2521 tagagggcgc tcggctgcca ggcatccccg gcatgcggac cgtactgggg gagcccggcg
     2581 gcccggaccg ccagcgcggt gatccggtgg gcgtgtacgt ggtccgggtg gccggtcatg
     2641 cctccgtagg cgtcccccgt gatcacgacc tccggccgga cgtcccggat gtggccgacc
     2701 accgcctcca cggcttcgtc cagcggagcg tcgaggaacc ggggcctgcc cggggccgac
     2761 tccggtaccc gggcgtccgc gtagccgagc aggcgcggag cgcccgcgcc cagggcgtcc
     2821 agggcccggg ccagctcggc ggcccggtgg gtgccccgcg cccaggtcgc ggtgaccacc
     2881 gccgtgcggg cgccggccgc cgcgtgccgg gccagtacgc ctccgcagaa caaggactcg
     2941 tcgtccgggc gtggacggcg aggatgctcg gcagaggcat ggggacgcct ttcgtgtcga
     3001 cggtgccggt cgtggatccc ggacggggcc gggagccctg ccggagttct gcccggtccg
     3061 gccgggtcgg cacacggccg cacgcaccgc cgtcgccggg cgcatgtgtg gactcggcag
     3121 gcgcgggcag ccgcaccggg agacggtgga ggaaagctcc atctgtcctt ttcgtgccgt
     3181 tgtggaggat cgcgatgacc cacccgtacc ccgaccccgt accgccctcg ccgacgcctc
     3241 cgcccgtacc gggcccgccc actccggtgc ccggtccgcc gcccgtgccg ccgggtcccg
     3301 ggcccgtgcc gcccggcccc gcgcccgacc cgatcccgca gccgccgggc cccgaccccg
     3361 taccggatcc ggaacccgag ccccagcccg ccgggacgtg agcgagggcc ggtaccggag
     3421 acggtgccgg ccccggtggt gatgaccgac cgtcgcccgg cgcttcccgg cccgccccga
     3481 taaagccgag gggcgccggg gcgccgggcg gcggtacgtt tcccagaagt ccgccccagg
     3541 catccgtccc gggcatcctt cccgggcatc cgtcccgctc aggcgccccg ctcagtcgct
     3601 ccgctcgacc agcgcgtccg cgtgcgcgag accgctctcc gctgcgatgg cgtccatcga
     3661 ctgcgcgtcc cgcaccgtcg cgaacgtcac cgcagccctg tcgcgccccg cccccgccgc
     3721 cacccggaac ccgtacaccg ccggccgcgg gaggctgttg tacgcccacg gggtggagaa
     3781 gtagtacgcc ccggtgtcgt acagcaccac cacatcgtcc tgggccagtt ccggcagctc
     3841 tctgccgtac gccaccacgt cgcccgcgaa gcagcacggt cccgcgatgt cctgcgccag
     3901 caccgggccg cccttcggcg tgccgtccgg gccgaacgcc ccgatccgca acggccaggc
     3961 ttcgggcatc aggacggtac gggtcgcggt ctgcgctccc gcgtgggtga gcgcgatacg
     4021 gcggcccccg gcgtccttcg tgtactccac ccgggccccg atgaacccgt tcttcgccag
     4081 cagcgaccgg ccgaactccg tgaccagcgc atagcgcccg gagaagaggc cgggggcggc
     4141 cgccgtcagg gccgccacat ggtccgcgta ggtggggtgc acggtctcgt cggcgaagtt
     4201 gaccgggagt ccgccgccga tgtcgagact ggtgacctgc cggacgccca gcgtcgcgtt
     4261 gatctcctcg gccagttcgt acgtctgcgc caccccggcc gcgatcagct ccaacgggca
     4321 gccctgggag cccacatggg catgcagccg ggtcagccac ggccgttccc ggaaggccgt
     4381 cacgatcccc tcgcgggccc ccgcatcgcg cagcgccacc ccgaacttcg aggtgtccgt
     4441 cgccgtgctc atcgccccga tcgcgccacc gccgacctga gggttgaccc gcagcccgag
     4501 caccgaggcg gtgcccgggt tccggagggc gtcgacgcgg cgcagttcgt cgaggctgtc
     4561 ggcgttgacg gccacgccga gggcgagcgc caaccggatc tcctcgcggg tcttggcggg
     4621 ggagtccagg acgatgcggg acgcggggaa cccggcctcc agggcgaggc gcagttcacc
     4681 ggggctggcc acctcgcacc ccatgccgca gtcggcgagc agccgcagca ccgggatcag
     4741 ggagcaggcc ttggccgcga aggtgtgcag taccccgggt gtggcggcga aggcgtcgtg
     4801 cagggctccg acggaggcac gcaccccctc cgtgtcgatg aatccggcga ccgggccgcc
     4861 ctcgcccagc agtccttgcc ggacggccgc ttcgacgacg gcgtcgaagc gggcggcggg
     4921 tggtgccgtg ccgtcctcgt ggtggggtgc ggaagtcgtc tgcgtcatac ggccagcgtc
     4981 cctgatggct gcccccgggg tcaggcgatc agaccgacca cggcctccgg cgtcagcatc
     5041 cggaaggtga aggcctgctg gaagtagagg tggatgctgg tggcgtcgtg gtccgcgtag
     5101 ccgatcgaca ggtcctggcc caggcagagt tcgaagtctc cgccccgggt ggagagcagc
     5161 accccgccct tgaccgcggg cgcccacagg atctcgtcgt ccaccaggcg gctcagatgc
     5221 gtcttgaccg gatacccgtg gtccgaggtc tcggccgcct cggtgaacgc gtccgcgccg
     5281 agcagcagcc ggtacggtcc gtcgacaccg gccaggcgca gccgcgtcac ggcctggctg
     5341 accgccaccg gatagtcccg cgcgtcggcg ggcagcggca gcggctcgtg cgaggaaccg
     5401 tcccgcaggc cggtgatccc ggccgccccg tacccgtcga tgatcgccat gtcctcggcg
     5461 aacgcacagg tgcgcgccgc gtccttgacg ggctgccagt cgctgtcggc ggagccgcgc
     5521 tccacgtcgt ccacggcctg acgggtcacc gtgaacggaa cccgccactc gatgaccggc
     5581 atcgaggtgc gggcccgcgc caccacgtcc ggggtgggcg gatcgatgtc gcgcagatgg
     5641 ccgtcgccca cggcggacag cgtggggccg tccggatcgg acacgtccac gacccggcgg
     5701 ccggcgacat ggcggcggaa ggtgcggcgg gcctcctcct cgatctcgtc ccaggcggac
     5761 ggggtgaccg gtgcgagttc gcggtgcaga ttgttcatca ctgggcactt tcttgcaggc
     5821 tgccgatgcg cagcgaaccg tggtcggggc cggccgcgac cggctcctgc cgggcggagg
     5881 caggggcggg tcccggctcc tgcaggaccg gcacgggcac cggttccggc cggtccccgg
     5941 agcgggccgg ggcgggcagg ggcggcgggg cgtcgaggaa gtcggcgcga ggggcgaaga
     6001 agagcgagcc cgtgaccgcg gtggagaagt cgaggatgcg gtcgtgcgtc ccgggcggat
     6061 cgccgaggaa catgttgcgg agcatccgct cggtcacgtc gggatcggcg gcgtacccga
     6121 tgaagtacgt accgaactcg cccttcccga agctcccgaa cggcatgttc gcccgcagga
     6181 tgtcgcgttc ggtgccgtcc gggtcggtga tggtgttgag cgcgaggtgg gagtcggcgg
     6241 gcttgtcctc gtcggggaac tccacgtcgt cgagcttggt gcggccgatg acgcgctcct
     6301 gctcctcgac ggagagcgcg ttccaggacg tcaggtcgtg cagatacttc tgcacgacga
     6361 cgtagctgcc gccctcgaag tccgggtcgt cgtccgcgcc gaccaacgcc gccgaacggg
     6421 cgtcgtcgcc gaccgggttc tccgtaccgt ccacgaagcc gagcagatcc cggtggtcga
     6481 ggtagcggaa gccgtgcgtc tcgtccacga tccgcagggc cccgccgagc ttgtcgagca
     6541 actgggccgc ccacgcgtag cagacgtcca tccgctcggc ccggatgtgc agcagcacgt
     6601 cacccggggt cgccggggcg tggtgcagag gccctcgcag ctccgggaac gggtggaggt
     6661 gcgcggggcg gggccccgcg aacaaccggt cccaggcgct ggagccgaag cccgccacac
     6721 aggcgaggcc ggagtccggg taccggaacc cgagcgaccg ggcgacggcc gcgagatccg
     6781 gcagcacgtc ccgcaccgcg ccttcgccgc ccggctcgat cgtgccgacc aggaacagcg
     6841 ccgcgctggt caacggcgcc acgacgggct ggacggagac ggggtcaggc acggcgtcgg
     6901 gcatgcgcgc actccactac gacggtgaaa cggggcttcg acgaacgacg aacaggactc
     6961 gcagccacta agccacgccc tgggtccggc cgcacccggg gtggaccgtg cgggtcagcg
     7021 gcgacgggcc gccggccgac gatcggcgga cgcgcaacct gaactgttcg aggtacccgg
     7081 gacagccgtg ttcacaggac agtcgggtgt gcccgaccgg gcaaggcacc catggccgcg
     7141 gatgccgacc acgcaccctt tcaggagccc ttccatggca ctcaccgcac cccgcaggcg
     7201 caccgcgatc gtgttcgcct ccctcgtcct gctgggaggc acggccgccg gcggcgtcgc
     7261 cgccgcggcc gagacaggcg ccgccgctgc cgcggcctac ccgtgcgacg tgaagatgag
     7321 cgccgccggc cggctctcgg ccggctacta cgacggaacc acggtcgtcc cctccacctc
     7381 gcaggtgacc gccgccggca aggaggccca gtgcctcctc tggtaccagg gccacaaccc
     7441 ggggacgatc gacggcatct tcggccgcaa ctcccaggcc gcggcgaaga agttccagaa
     7501 gggcgtcaac tacgcctgcc gcaccaccct ggccgaggac ggcaaggtcg gtcagcagac
     7561 gtggccctat ctgcgacggc tctactgctg agcccgcggg ccggacccgg gcgcggacag
     7621 gtcagctttc cacttcggtc ctgtcgccgc cccagagcgt gtggaacgag ccctcccggt
     7681 ccgtacgccg gtaggtgtgc gccccgaaga agtcccgctg gccctgggtg agcgccgcgg
     7741 gcagccggtc cgcgcgcagc ccgtcgtagt acgagagcgc ggcggcgaag cccggggtcg
     7801 gcacgccctg gcgtacggcc tcggccacca ccgcccgcca gtcgtcctgc gccgcgccga
     7861 tctcctgccc gaactccttg tcggagagca ggctgaccag gtcgggccgg gagtcgtagg
     7921 cggcgcggat gcggtcgagg aacgccgccc ggatgatgca gcccgcccgc cagatcgccg
     7981 ccaccgagcc gagatcgatg tcccagtcgt aggtctcgcc gcccgccctg atctggtgga
     8041 aaccctgcgt gtacgagacc accttggagg cgtacagggc ctgttccacc tggtccgcga
     8101 acgccgctgc cgccgccccg tccagcggct tcggggtggg ccccgggaga ccgcgggccg
     8161 cctcccgcag atcggcgtgg ccggacagcg agcgggcgaa gaccgcctcc gcgatgcccg
     8221 acaccgggac cccgaggtcg agcgcgatct gtacggtcca gcgcccggtg cccttctgct
     8281 ccgcccggtc ctgcacgatg tcgacgaacg gcttcccggt cgccgcgtcc gtatgggcga
     8341 ggacctccgc cgtgatctcg atcagatacg aatccagccg gccggtgttc cacgtacgga
     8401 agacgtcggc gatctgcgcg ggggagtacc cggccaccgc gcgcagcagg tcgtacgcct
     8461 cggcgatcag ctgcatgtcc gcgtactcga tgccgttgtg caccatcttc acgaagtggc
     8521 ccgcgccgtc cggaccgatg tgcgtggtgc acggcgtgcc gtccttcgcc tgcgcggcga
     8581 tcttctccag catcggcccc agcgacgcgt aggactccgc cgagccgccc ggcatgatgc
     8641 tcggtccgtt cagcgcgccc tcctcgccac cggagatccc caccccgacg aagtggatcc
     8701 cgcgctcgcg cagttccttc tcacggcggc gggtgtcctc gaagtgggcg ttcccgccgt
     8761 cgatgatgac gtcgccctcc tccagcagcg gcgcgaactc ctggatcacc gcgtcggtgg
     8821 ggtcgcccgc cttcaccatg atcaccagac gccggggccg ctccagcgcg gcgacgaact
     8881 cctcgggcgt gtgcgcggcc acgaacgtgc cctcgtcgcc gaactcctcg accagggcgt
     8941 ccgtccggga cgtggtgcgg ttgtgcacgg cgacggtgaa gccgttgcgg gcgaagttcc
     9001 gggccaggtt gcggcccatg accgcgagtc cggtgacgcc gatctgggcg gtgccgctca
     9061 ttggtgcgct cctggggtgc gagtacgtcc gtgcgtgcgg tgggtgcggt gggtgcggta
     9121 cgggggagtg cgtgggggcc ggtgtgcgct tcggtcaggg gagtacgggc ccggggaccg
     9181 ccgcgttcag gggcgggcgc tcgccgaccg gtcagctcag gacggcggcg gggctgccgt
     9241 gccgccgccg gcggggccgg aaccgggggc ggcgggcggc gggccggcct ccagcagcgc
     9301 gtcgtcgtcc cccggctcgg ggatcacatg catcgcggcc tcctcggccg acgccgccgc
     9361 gccgtcgatg cccacgtcac tggcggtgag ggtggccggg acgcgcccgt cggcctgctg
     9421 ggtgagccgc ccggcgcggg cgacgcccac ctcgtcgtcc cagggctctc cgtcgccgtc
     9481 caccagatcg cccacaccgt cgccggcgga accgtccggc tcgggcactt ccctggtcag
     9541 ccgcgtttcg agggactccc ctgtgttccg ctcgtcggcc gtggtgccga gtccgtcgac
     9601 cacccacggg cggtccggcg gcgaataccc ctcgtccagg gggtcgccgg tgccggacgg
     9661 atccatgagc gtgtcctcgg cgccgagctg ttcgtcggga tcggaagcct cgggttcctg
     9721 gggctggtag acgtcgtccc cccagtcggt gtcgctcatg gcgcgtcctt tcctccggtc
     9781 ctccgggagt gacgggttct cctgtcccct gtacgcaatt ccactcccgt cccgctccgg
     9841 ccgcaaaggg aagatcggtt tccggagccg ggctcgggcg gtgcgaaccg gtccgtacgc
     9901 aggtcgcggc cggttcgccg cagaagcgag gagttcgtgg gagtccgggc acaggctgtt
     9961 cgaatcgtcg aacggccggg catagggtga tctcccatgg ggaacggaca gcagtcgccc
    10021 gggggcgatc accaccagca gggtcagccg cagcagccgt acggacaggg ccaggggcag
    10081 cagccgtacg ggtggcctcc gcagggacag caggccggcg ggcagccgcc gtacgcggga
    10141 gccgggaccg ctccgtggga cgcgcgcacc gaacctgccg ggcagcccgc gtccgccccc
    10201 tggagcgcgc ggaccgcttc ctccgggccg cccccgcccg gcggcagccg ccgtagctcg
    10261 gtcatcgccg tggtcgcggc cgcggccgtc gtcatcgccg ccggaatcac cggattcctg
    10321 gtgctcggcg gcgacgagga cgacgggaag ccggtcgcgg gacccaccgg gacggacacc
    10381 gtgtcaccgt cggcgtcccc gggcgaggcc cggggcggtg ccgagaacgg gcccgcgccc
    10441 ctcgtacccg gctggaagac cgtggtcaac cccaagcgcg gcatcgcctt cgacgtgccc
    10501 gtccagtggg cgctcaagtc gaccggctgg gtgagttacg tcgccgagaa cgacgacccc
    10561 gaggagatgc cgctcgtcgg gttctcggcc ccggcgatcc tccaggagaa gtggtgccgg
    10621 tccgacgacg acggcaacgg cacccaggag gacactccgc tggcctccgc gggctcccgg
    10681 ggcgagccgg acgcccgaag caccgccgaa gcggcccggg agaacgcccg cctgtgggtc
    10741 tacggcagct acgcccagcc gtcgaaggag aaggtcacca ccggggcggc gaagccgt
//
