LOCUS       NZ_CP079114            10375 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. WY228 chromosome.
ACCESSION   NZ_CP079114
VERSION     NZ_CP079114.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WY228
  ORGANISM  Streptomyces sp. WY228
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8286721)
  AUTHORS   Qin,S.
  TITLE     Volatile organic compounds produced by endophyte Streptomyces sp.
            WY228 mediate plant growth and salt stress tolerance by activating
            hormonal signaling and anthocyanin biosynthesis,
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8286721)
  AUTHORS   Qin,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2021) Key lab of biotechnology of medicinal
            plants, Jiangsu Normal University, No. 101 Shanghai Road, Xuzhou
            221116, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019287315.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 06:02:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,303
            CDSs (total)                      :: 7,213
            Genes (coding)                    :: 7,059
            CDSs (with protein)               :: 7,059
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 83 of 155
            Pseudo Genes (incomplete)         :: 99 of 155
            Pseudo Genes (internal stop)      :: 10 of 155
            Pseudo Genes (multiple problems)  :: 36 of 155
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:35:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7014652
            Orig. end                         :: 7025027
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079114.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10375
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="28"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10375
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10375
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5375](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5375
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(197..211)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.07"
                     /tool="antismash"
     misc_feature    268..283
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            458..1303
                     /locus_tag="KV381_RS30750"
                     /old_locus_tag="KV381_30750"
     CDS             458..1303
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013722.5"
                     /locus_tag="KV381_RS30750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_30750"
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="WP_218786808.1"
                     /transl_table=11
                     /translation="MTTPASAPAPSAPLLDAAAAHRHRAVTRRALGRPAPGRAEAGPLA
                     AFVLTHPLAERTVAGLAAGHGPYSLVHLAQEIELHHPPRPGAPLDASAALDAVRVEAKG
                     TRLVLTTTLTDRATGAEQARLTAHILLAGIRAAEPHGTLVPTASARPQTPGDTVSRDLA
                     IDRGWIADYGHAAGDLNPVHLDPEAARAAGFADVIAHGMSLVALAVEEAAERYADGDVD
                     RVRAIGARFARPVLPGEATTLDLTPSAAGDTVAFTVRSGGAAAVKGGWLRLGPGTGAGR
                     "
     gene            1300..2799
                     /locus_tag="KV381_RS30755"
                     /old_locus_tag="KV381_30755"
     CDS             1300..2799
                     /NRPS_PKS="Domain: AMP-binding (15-397). E-value: 3.1e-70.
                     Score: 228.8. Matches aSDomain:
                     nrpspksdomains_KV381_RS30755_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404417.1"
                     /locus_tag="KV381_RS30755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_30755"
                     /product="AMP-binding protein"
                     /protein_id="WP_032786159.1"
                     /sec_met_domain="AMP-binding (E-value: 2e-63, bitscore:
                     213.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRTDRHGETAESVLRWADKTPDAPALWWNGSPTSYRELADATAA
                     ARARLAEHVPAGATVAVLDEKSPGTVATVLACLATGRPALLPSPTLPPDHVAAVMAEAG
                     CRYALAGPAVTARDPEHGEGGGGAVPPGTALVLTTSGSTGIPKAVPLPHRSVEAFTGWA
                     ADAFGIAETTPVLNYAPLNFDLCLLDVWTTLAHGGQVVLVDPATAASGRGLLRLLRTHR
                     VEVVQAVPLFYALVGTQVRVEGGGPLETVRHAAFTGDVMPEQALAELMDLAPGARLHNI
                     YGCTETNDSLIHEVDRSLPQSAPMPLGRPLPGVRLRLRNSDGRLLTGPGRGELEVSTPF
                     QSTGYADPRRAAEKFVLDPAADGGPGTRWFRTGDLVEQDEDGTLRLVGRLDHQVKIRGV
                     AVNTGEVEQVLLEHPEVLEAGVTTVPDPVEGRRLIAVVRLKDGSTANSLDLKRHCTARL
                     ARGWVPGLLVPVTEPLPRTSTGKVDRRAITRTAAAPAATAS"
     aSDomain        1345..2490
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KV381_RS30755_AMP-binding.1"
                     /evalue="3.10E-70"
                     /label="KV381_RS30755_AMP-binding.1"
                     /locus_tag="KV381_RS30755"
                     /protein_end="397"
                     /protein_start="15"
                     /score="228.8"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="RWADKTPDAPALWWNGSPTSYRELADATAAARARLAEHVPAGATV
                     AVLDEKSPGTVATVLACLATGRPALLPSPTLPPDHVAAVMAEAGCRYALAGPAVTARDP
                     EHGEGGGGAVPPGTALVLTTSGSTGIPKAVPLPHRSVEAFTGWAADAFGIAETTPVLNY
                     APLNFDLCLLDVWTTLAHGGQVVLVDPATAASGRGLLRLLRTHRVEVVQAVPLFYALVG
                     TQVRVEGGGPLETVRHAAFTGDVMPEQALAELMDLAPGARLHNIYGCTETNDSLIHEVD
                     RSLPQSAPMPLGRPLPGVRLRLRNSDGRLLTGPGRGELEVSTPFQSTGYADPRRAAEKF
                     VLDPAADGGPGTRWFRTGDLVEQDEDGTLRLVGRLDHQVKIR"
     CDS_motif       1699..1758
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KV381_RS30755_0001"
                     /evalue="1.70E-05"
                     /label="NRPS-A_a3"
                     /locus_tag="KV381_RS30755"
                     /protein_end="153"
                     /protein_start="133"
                     /score="17.5"
                     /tool="antismash"
                     /translation="ALVLTTSGSTGIPKAVPLPH"
     CDS_motif       2458..2520
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KV381_RS30755_0002"
                     /evalue="8.10E-08"
                     /label="NRPS-A_a8"
                     /locus_tag="KV381_RS30755"
                     /protein_end="407"
                     /protein_start="386"
                     /score="24.6"
                     /tool="antismash"
                     /translation="VGRLDHQVKIRGVAVNTGEVE"
     gene            2896..3159
                     /locus_tag="KV381_RS30760"
                     /old_locus_tag="KV381_30760"
     CDS             2896..3159
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1147: putative acyl carrier protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064088.1"
                     /locus_tag="KV381_RS30760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_30760"
                     /product="acyl carrier protein"
                     /protein_id="WP_030084126.1"
                     /sec_met_domain="PP-binding (E-value: 4.1e-05, bitscore:
                     22.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNADQISEYIVREFLPDLTPSQLPHDMDLLADGALDSLGVLKLI
                     AWVEHHFRLAVGDTDLDPENFRSVNAIDAYIARSRSTAATGG"
     gene            3163..4185
                     /locus_tag="KV381_RS30765"
                     /old_locus_tag="KV381_30765"
     CDS             3163..4185
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064086.1"
                     /locus_tag="KV381_RS30765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_30765"
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="WP_030625152.1"
                     /transl_table=11
                     /translation="MHPALVPMVDRRAPAGPEQWDALWQALADGALDRGDALALLASLT
                     AALPEEDSLLALLDALDRRRPARPATPWPGTVNVVGTGGGPPTVNLSTAAALVAAACGV
                     RVVKSGSRAHTARTGSVDLLDRLGVPFAASLDQASGQLDAHGIVFTGPFVYPVQLARLA
                     LLAVPTPMRVFGRFLNTLGPFLAAVPVAAQVTGVSGGRVPPALRAVAARPGAPATWLVT
                     NPLGADELLSICDNTVHLPDGGTLTLRAGDLVPAGGSPDDLAPVPREAAAEHFRTVLAG
                     RAGGPLRATLQLNAAALALAAGHRDDWAKAVAEAGAALDDGAALDLLDRLKGKGAGRAG
                     "
     gene            4175..5014
                     /gene="trpA"
                     /locus_tag="KV381_RS30770"
                     /old_locus_tag="KV381_30770"
     CDS             4175..5014
                     /EC_number="4.2.1.20"
                     /GO_function="GO:0004834 - tryptophan synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064084.1"
                     /locus_tag="KV381_RS30770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_30770"
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="WP_030625149.1"
                     /transl_table=11
                     /translation="MPDEFFAEARAREELGLAVFLNAGDPPLPVLTDLVAQLDASGVDC
                     LELAVPFPDSPTDGPVVRRSADRALTRGVGLRETLAFVAAVRPGLKRLRIAVLADWSHS
                     LRTAAPADYARAVADSGADALLAHGLPPRLRPAHHEALHAAGLPEVTTCYPASAPATVA
                     EAAAHATGYLYLVARYGRSGAGTPDGGHAGLAPTVAALRDLTASPIAVGFGVSTAEDLA
                     SVARSGADAAIVGSAGVAAVERSLEAGSDPVDGYRSFVAALGRPHTARPKEDPRAHP"
     gene            5001..5375
                     /locus_tag="KV381_RS30775"
                     /old_locus_tag="KV381_30775"
     CDS             5001..5375
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105936.1"
                     /locus_tag="KV381_RS30775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_30775"
                     /product="ectoine synthase"
                     /protein_id="WP_030625146.1"
                     /sec_met_domain="ectoine_synt (E-value: 9.1e-30, bitscore:
                     102.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLIRNIETVKTVDWGNGLSRRFILDADGLGYSVTDTTVRAGTKSR
                     LEYRNHLETCYCIEGSGEVVELDGTSHPLTPGVLYSLDNNDPHFLIASPHEDLRLVCVF
                     APALQGDEVHSLAEGVSSAY"
     gene            5520..6665
                     /locus_tag="KV381_RS30780"
                     /old_locus_tag="KV381_30780"
     CDS             5520..6665
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064082.1"
                     /locus_tag="KV381_RS30780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_30780"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_030084135.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 1.1e-17, bitscore:
                     63.5, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 3e-24, bitscore:
                     83.8, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 5.2e-28, bitscore:
                     96.9, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRNRDADQQALCAGMAQWGKILSEGHIEADARSAFEPDRWRSLAD
                     SGVLGLPFPQQWGGAGQSLLTTLHVLETLGETCRESGLNFCATTSMASTGVPLTAFGSD
                     ALRERYLPGICSGELIGAHAITEPDSGSDAMAMTTRAERDGTHYVLTGGKAFVSNGPIA
                     DVFVVYARTRPEGGPLGTTAFLVDRDTPGLSVGGTTAKMGLRTAPLSTLHLDGCRIPAD
                     RVLGRVGGGLLVLDHVMKREILFGFTVQLGEMRHRLERTVGYARERRAFGHPIGDYQAV
                     SHRVVDMKIRTDTAAKWLYDTAERLAAGEDVTADLAITKILASEAALSTSLSAVRVFGG
                     AGYLAEVGLEKEVRAAVAGTLYSGTNDIQYNHVASALGLGG"
     gene            6662..7354
                     /locus_tag="KV381_RS30785"
                     /old_locus_tag="KV381_30785"
     CDS             6662..7354
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105934.1"
                     /locus_tag="KV381_RS30785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_30785"
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="WP_098025489.1"
                     /transl_table=11
                     /translation="MSTPPRPLLKVCGATAPEEAAAVAPRADLVGLWYGVENGARDLTG
                     ARLTELADAVRTAGRAEPVLVTFLHDAERIAAAARAARVRWIQLHAYQPPAAVAALRAA
                     LPDAVLVKAVHVLDGACVERPFLTAYARAGADLFLVDAATRGGAVGSTGHRVPAEALEA
                     LLPVLPRPFILAGGLGEADAPGHAFAAHAGFRGVDIDHAARADAGLLDPARVAALDRAW
                     TGTPAPAP"
     gene            7403..8215
                     /locus_tag="KV381_RS30790"
                     /old_locus_tag="KV381_30790"
     CDS             7403..8215
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064080.1"
                     /locus_tag="KV381_RS30790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_30790"
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="WP_218786809.1"
                     /transl_table=11
                     /translation="MTQPFLSALLGADRPVIMEVKSRDAHGADLLRGRTPGDIAAAYQR
                     AGAPCVSVVTGRWFGGSAAMLHEVTARVDVPVLLKDFVTRHDQVTAAARAGVSAVLLTA
                     ALLPRESLHDLARACRAHGLTPFVEITDAQEAIGLPDPQDCVVAVNNKDITARERGAGD
                     LDRSRALLPAVRAAGTLCPVSASAVGDPATAAGLLAGGYRGLLIGTALLRAPSVDAWLA
                     EFDAHRAAGTEETGRVTVPLTVEAPPVLPHPAPVTGPAVAGEAEDVPA"
     gene            8212..9153
                     /locus_tag="KV381_RS30795"
                     /old_locus_tag="KV381_30795"
     CDS             8212..9153
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037169.1"
                     /locus_tag="KV381_RS30795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_30795"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_256995181.1"
                     /transl_table=11
                     /translation="MSAAPTESVATADVTAAGHDAAPGGVEQIVLGHGRHRLGAWRAEP
                     ATGAPRSVLVAVHGRAMSPGYFAGPVAPHTSLLALATSLGHTVLAVERPGYGLSRAALP
                     HGLPLAEQALLLRRSLADYAGLHPVGAGFFLLGHSDGGKTALHLAGEPWTGATPADRLL
                     GLDLNGCGWHYAPAAAHFPDTLAGGAGELNWGPLRLYPGGTFRASRALLREVPAAEEAE
                     TGRWPARFPAAAARVRCPVRLTFGAYEGWWRLDRDELAAVAASFTGTRRPAVERLPEAG
                     HNLSLGLAAPLYHARALAFLEECLAASSGGRG"
     gene            9304..9891
                     /locus_tag="KV381_RS30800"
                     /old_locus_tag="KV381_30800"
     CDS             9304..9891
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064078.1"
                     /locus_tag="KV381_RS30800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_30800"
                     /product="DUF1772 domain-containing protein"
                     /protein_id="WP_218786810.1"
                     /transl_table=11
                     /translation="MSHYPPHATPYQAGPVRPGGHPPAGDRITAPLLVASVLCTGLMAG
                     LFFAYDISVMPGLAELDDAAYAAAMQRFNAAIDGSALFGLVFLATLGLTVAAAIVAFRR
                     KRRAVALPLAAAALCYLLVLVVTVAVNLPLNADLAALGGSASAQDVHAVIHDFKSVWVP
                     VNIFRTLFCVLSLGALCAALLRYGKPETPVLR"
ORIGIN
        1 tcgccggata cgccgccacc tcggacgccc accacctctc ggccccgcac cccgacgggg
       61 cgtacgccgc cgaagccatg accggcgcgc tcgccgacgc cggtctcgcc ccctcggaca
      121 tcgcccacgt caacgcccac ggcaccgcca ccgtgcacaa cgaccgcgcc gaagccaccg
      181 ccctggccaa ggtgttcggg ccggacggcc cgccggtcac cgccagcaag ggcgtgctgg
      241 gccacctcat cggagcggcc ggagccgtcg aactcgtggt cgccgtgctg tcgatgaacg
      301 ccggtgcggt gccgcccacc gccaaccaca cacgaacgga acccgagatg gagatcgacg
      361 tggtccacgg caccccgcgg cctgtcccca ccggccccgc gctgaccaac tccttcggct
      421 tcggcggcca caacagcagc ctggtggtga cccccgcatg acgacccccg cctccgcgcc
      481 cgccccgtcc gcaccgctcc tcgacgcggc cgccgcccac cgccaccgcg ccgtcacccg
      541 ccgggcgctg ggccgcccgg cgcccggccg ggccgaggcg gggcccctcg ccgccttcgt
      601 cctcacccat ccgctcgccg agcgcacggt cgccggcctc gccgccggcc acggcccgta
      661 ctcgctggtc cacctcgccc aggagatcga gctgcaccac ccgccgcgcc ccggcgcccc
      721 gctcgacgcg tccgccgcac tcgacgccgt acgcgtcgag gccaagggca cccggctcgt
      781 cctgaccacc acactcaccg accgggcgac cggcgccgaa caggcccggc tcaccgccca
      841 catcctgctc gccggcatcc gagcggccga accgcacggc accctcgtcc ccacggcatc
      901 ggcgcgtcca cagacaccgg gcgacaccgt cagccgcgac ctcgcgatcg accgcgggtg
      961 gatagccgac tacgggcacg cggccggcga cctcaacccc gtccacctcg acccggaggc
     1021 cgcccgggcg gccgggttcg ccgatgtcat cgcccacggc atgagcctcg tggcactcgc
     1081 cgtggaggag gccgccgaac ggtacgcgga cggagacgtc gaccgggtcc gggcgatcgg
     1141 cgcgcgcttc gcccgcccgg tcctgcccgg cgaggcaacg accctcgacc tcacgccctc
     1201 cgccgccggg gacaccgtcg ccttcaccgt acggtccggg ggcgccgcgg ccgtcaaggg
     1261 cggctggctc cggctgggcc ccgggacggg agcgggccga tgacccggac cgaccgccac
     1321 ggcgagaccg ccgaatccgt gctccgctgg gccgacaaga ccccggacgc ccccgccctg
     1381 tggtggaacg gcagccccac cagctaccgg gaactggccg acgcgaccgc cgccgcccgc
     1441 gcccggctcg ccgaacacgt ccccgccggg gccacggtcg ccgtgctcga cgagaagtcg
     1501 ccaggcaccg tcgccaccgt cctggcctgc ctggccaccg gccgccccgc actgctgccc
     1561 tcgcccaccc tgccgcccga ccacgtcgcg gcggtgatgg cggaggcggg ctgccggtac
     1621 gcgctcgccg gtcctgccgt cacggcgagg gatccggagc acggcgaggg cggcggggga
     1681 gcggtgccgc cgggcaccgc cctcgtcctg accacctccg gctccaccgg catccccaag
     1741 gcggtcccgc tgccgcaccg gtccgtggag gccttcaccg gctgggccgc ggacgccttc
     1801 ggcatcgccg agaccacccc ggtgctcaac tacgccccgc tcaacttcga cctctgcctg
     1861 ctggacgtct ggaccaccct cgcccacggc ggccaggtgg tcctggtgga cccggcgacg
     1921 gcggccagcg gccgggggct cctgcgcctg ctgcgcaccc accgcgtcga ggtggtccag
     1981 gcggtcccgc tcttctacgc cctggtcggc acccaggtcc gggtggaggg cggcggcccg
     2041 ctggagaccg tccggcacgc ggcgttcacc ggcgacgtga tgcccgagca ggcgctcgcc
     2101 gaactcatgg acctcgcccc cggcgcccgg ctgcacaaca tctacggctg caccgagacc
     2161 aacgacagcc tgatccacga ggtcgaccga agcctcccgc agtccgcccc gatgccgctg
     2221 ggccgcccgc tccccggcgt acgcctgagg ctgcggaact ccgacggccg gctgctgacc
     2281 gggcccggac gcggcgaact ggaggtctcc acccccttcc agagcaccgg ttacgccgat
     2341 ccgcgccggg ccgccgagaa gttcgtcctg gacccggcgg cggacggcgg tccgggcacc
     2401 cggtggttcc gcaccggcga cctggtcgag caggacgagg acggcactct ccggctcgtc
     2461 ggccggctgg accaccaggt gaagatccgc ggcgtcgccg tcaacaccgg cgaggtcgaa
     2521 caggtgctgc tggaacaccc cgaggtgctg gaggccgggg tgaccaccgt ccccgacccg
     2581 gtcgagggcc gccgtctgat cgccgtcgta cggctgaagg acggctccac cgccaacagc
     2641 ctcgacctca aacggcactg caccgcccgg ctggcccgcg gctgggtacc gggcctcctc
     2701 gtccccgtga ccgagcccct gccgcgcacc agcaccggca aggtggaccg ccgcgcgatc
     2761 acccggaccg cggccgcacc cgccgccacc gcgtcctgac ccggaccgac cgtcctccgc
     2821 ggaccgcccg gcagccgccg acaggccccg cgccacccga gaaccccgta cgagcaaccc
     2881 gccaaggagc gccccgtgag caacgccgac cagatcagcg agtacatcgt ccgggagttc
     2941 cttcccgacc tcaccccctc ccaactcccg cacgacatgg acctcctggc cgacggcgcg
     3001 ctcgacagcc tcggcgtcct caagctgatc gcctgggtcg agcaccactt ccggctggcg
     3061 gtcggcgaca ccgacctcga ccccgagaac ttccgcagcg tcaacgccat cgacgcctac
     3121 atcgcgcgct cccggagcac cgccgccacg ggaggctgag ccgtgcaccc ggccctcgtc
     3181 ccgatggtcg accggcgcgc cccggccggc cccgagcagt gggacgcact ctggcaggcg
     3241 ctggccgacg gagcactgga ccgcggtgac gcgctggcgc tcctcgcctc cctcaccgcg
     3301 gccctgcccg aggaggacag cctgctcgcc ctgctcgacg cgctggaccg gcggcgcccg
     3361 gcgcggcccg cgacgccctg gcccggcacc gtcaacgtgg tgggcaccgg cggcggtccg
     3421 cccaccgtga acctgtccac ggccgccgcg ctggtcgccg ccgcctgcgg ggtccgggtc
     3481 gtcaagtccg gctcccgcgc ccacaccgcg cgcaccggtt cggtcgacct cctggaccgg
     3541 ctgggcgttc cgttcgccgc ctccctcgac caggcgtccg gccaactgga cgcccacggc
     3601 atcgtcttca ccgggccgtt cgtctacccc gtgcagctgg cccggctcgc cctgctggcc
     3661 gtgcccacgc ccatgcgggt gttcggccgg ttcctcaaca ccctgggccc gttcctcgcg
     3721 gccgtcccgg tggccgccca ggtcaccggc gtcagcgggg gccgcgtccc gcccgccctg
     3781 cgggccgtgg ccgcccggcc cggcgccccg gcgacctggc tggtgaccaa cccgctcggc
     3841 gccgacgaac tcctcagcat ctgcgacaac acggtccacc tccccgacgg cggcacactg
     3901 acactccgcg ccggcgacct cgtccccgcg ggcggatcac ccgacgatct cgcccccgtc
     3961 ccccgggagg ccgccgccga gcacttccgt accgtcctcg ccggccgggc gggcggtccc
     4021 ctgcgcgcca ccctccagct caacgcggcc gcgctcgccc tggcggccgg tcaccgcgac
     4081 gactgggcga aggcggtcgc cgaggcgggc gccgcgctcg acgacggggc ggcgctcgac
     4141 ctgctggacc ggctgaaggg gaagggtgcg ggccgtgccg gatgaattct tcgccgaggc
     4201 ccgcgcccgc gaggagctgg gcctggcggt cttcctcaac gcgggcgacc cgccgctgcc
     4261 cgtcctcacc gacctcgtcg cgcagctgga cgcgtcgggc gtcgactgcc tcgaactggc
     4321 cgtccccttc cccgactcgc ccaccgacgg gcccgtcgtg cgccggtccg ccgaccgggc
     4381 gctgacccgg ggcgtcgggc tgcgcgagac cctggccttc gtcgccgccg tacggccggg
     4441 gctgaagcgg ctgcggatcg cggtgctcgc cgactggagc cacagcctgc gcaccgcagc
     4501 gcccgccgac tacgcccgtg ccgtcgcgga cagcggcgcc gacgccctgc tggcccacgg
     4561 tctgccgccc cggctgcggc cggcccacca cgaggcgctg cacgcggcgg gcctgcccga
     4621 ggtcaccacc tgctatccgg ccagcgcacc ggccaccgtc gccgaggcgg cggcccacgc
     4681 caccggatac ctctacctgg tcgcgcgcta cgggcgcagc ggcgccggaa cgcccgacgg
     4741 cggccacgcc ggtctggcgc cgaccgtcgc cgccctgcgc gacctcaccg cctccccgat
     4801 agccgtcggc ttcggggtgt ccaccgccga ggatctcgcc tccgtcgccc gcagcggcgc
     4861 ggacgcggcg atcgtcggct ccgccggagt ggccgcggtg gaacggagtc tggaggccgg
     4921 gtccgacccg gtcgacggct accgctcctt cgtcgccgcc ctcggccgtc cccacaccgc
     4981 ccgtccgaag gaggaccccc gtgctcatcc gtaacatcga gaccgtcaag accgtcgact
     5041 ggggcaacgg actcagccgc agattcatcc tggacgccga cggcctgggc tacagcgtca
     5101 ccgacaccac cgtgcgtgcc gggaccaagt cccgcctgga gtaccgcaac cacctggaga
     5161 cctgctactg catcgagggc tccggcgagg tggtggaact cgacggcacc agtcatccgc
     5221 tgacgccggg cgtcctctac tcgctcgaca acaacgaccc gcacttcctg atcgcctcgc
     5281 cgcacgagga cctgcgtctg gtctgcgtct tcgccccggc gctccagggc gacgaggtgc
     5341 acagcctggc cgaaggcgtc tcctcggcct actaggcctg tccgggcagc acagccgctc
     5401 acccgacggg cgcctgaacc aggggcgccc gccgacctga tgacgacggg acaccgccgt
     5461 cacccctccg cccgtcatcg accgaggcca cccgcacggc ccgtacggaa ggctccgcca
     5521 tgagaaaccg cgacgcggat cagcaggcgc tctgcgccgg catggcccag tggggcaaga
     5581 tcctgagcga aggacacatc gaggcggacg cccggtccgc gttcgaaccg gaccgctggc
     5641 ggtccctcgc cgacagcgga gtcctcgggc tgccgttccc ccaacagtgg ggcggcgcgg
     5701 gccagtccct tctgaccacc ctgcacgtcc tggagaccct cggcgagacc tgccgggagt
     5761 ccgggctgaa cttctgcgcc accacgtcga tggccagcac cggcgtcccc ctgaccgcct
     5821 tcggcagcga cgccctgcgc gagcgctatc tgcccggcat ctgctcgggc gagctgatcg
     5881 gcgcgcacgc catcaccgag cccgacagcg ggtccgacgc catggcgatg accacccgtg
     5941 ccgagcgcga cggcacgcac tacgtcctca ccggcggcaa ggccttcgtc agcaacggcc
     6001 ccatcgccga cgtcttcgtc gtctacgcgc gtacccgacc ggagggcgga ccgctcggca
     6061 ccaccgcctt cctcgtcgac cgggacaccc ccgggctctc cgtcggcggg acgaccgcca
     6121 agatgggcct gcgcaccgcc ccgttgtcca ccctccacct cgacggctgc cggatacccg
     6181 ccgaccgggt gctgggccgg gtcggcggcg gactgctcgt cctggaccac gtgatgaagc
     6241 gcgagatcct gttcggcttc accgtccagc tcggcgagat gcgccaccgg ctggagcgca
     6301 ccgtcggcta cgcccgggaa cgccgggcct tcggccaccc catcggcgac taccaggcgg
     6361 tctcgcaccg cgtggtcgac atgaagatcc gcaccgacac cgccgccaag tggctctacg
     6421 acaccgccga gcggctcgcc gcgggcgagg acgtcaccgc cgacctcgcc atcaccaaga
     6481 tcctcgccag cgaggccgcc ctgtccacca gcctctcggc ggtccgcgtc ttcggcggcg
     6541 cgggctacct cgccgaggtc ggcctggaga aggaggtacg cgccgcggtc gcgggcaccc
     6601 tctactccgg gaccaacgac atccagtaca accacgtcgc ctcggccctg gggctgggcg
     6661 gatgagcacc ccgccccggc cactgctcaa ggtgtgcggc gccaccgccc ccgaggaggc
     6721 cgccgccgtc gcgccacggg ccgatctcgt cggcctctgg tacggggtgg agaacggggc
     6781 ccgcgacctg accggagccc gactgaccga actggccgac gccgtccgca cggcgggccg
     6841 ggccgagccg gtgctcgtca ccttcctgca cgacgccgaa cggatcgccg ccgccgcccg
     6901 cgccgcccgg gtgcgctgga tccagctcca cgcctaccag ccgcccgcgg ccgtggccgc
     6961 cctgcgcgcg gcccttcccg acgcggtgct ggtcaaggcc gtgcacgttc ttgacggggc
     7021 gtgcgtcgag cggccgttcc tgacggcgta cgcccgcgcc ggagcggacc tgttcctcgt
     7081 cgacgcggcg acccggggcg gggcggtcgg cagtaccggc caccgcgtgc cggccgaggc
     7141 gctggaggcc ctgctgcccg tcctgccccg gccgttcatc ctcgccggcg gcctcggcga
     7201 ggccgacgca ccgggtcacg ccttcgccgc gcacgcgggg ttccggggcg tcgacatcga
     7261 ccacgccgcc cgtgcggacg ccggccttct cgacccggcc cgggtcgccg cgctggaccg
     7321 ggcctggacc ggcacacccg cccctgcgcc gtgacgtccc ccgccccgca agagaagccc
     7381 gccactccgt gaggaaagcc cgatgacgca acccttcctc tccgcgctcc tcggcgcgga
     7441 ccgtcccgtg atcatggagg tgaagtcccg cgacgcgcac ggcgccgacc tgctgcgggg
     7501 ccgcaccccc ggcgacatcg ccgccgccta ccagcgggcc ggagccccgt gcgtgtccgt
     7561 cgtgaccggc cgctggttcg gcggttcggc cgccatgctg cacgaggtga cggcccgcgt
     7621 ggacgtcccg gtgctgctca aggacttcgt gacacggcac gaccaggtca ccgccgccgc
     7681 ccgagcgggc gtctcggcgg tcctgctgac cgcggcgctg ctgccccggg agtccctgca
     7741 cgacctggcc cgcgcatgcc gggcgcacgg gctgaccccg ttcgtggaga tcaccgacgc
     7801 ccaggaggcg atcggtctgc cggacccgca ggactgcgtc gtcgccgtca acaacaagga
     7861 catcaccgct cgcgaacggg gtgcgggaga cctggaccgc agccgtgcgc tgctgcccgc
     7921 cgtacgggcc gcgggcacgc tctgcccggt cagcgccagc gcggtgggcg accccgccac
     7981 cgccgccggt ctgctggccg ggggctaccg gggcctgctc atcgggaccg cgctgctgcg
     8041 ggcgccgagc gtggacgcct ggctggccga gttcgacgca caccgcgccg cgggcaccga
     8101 ggagaccggg cgggtcacgg tgccgctcac cgtcgaagcg cccccggtcc tgccgcaccc
     8161 cgcccccgtg accggccccg cggtcgcggg cgaggccgag gacgtgcccg cgtgagcgcc
     8221 gcgccgacgg agtcggtggc cacggcggac gtcaccgcgg ccgggcacga cgccgctccg
     8281 ggcggcgtgg agcagatcgt gctgggccac ggccgccacc gcctcggcgc ctggcgggcc
     8341 gaaccggcca ccggtgcgcc caggtcggta ctcgtcgccg tccacggccg ggccatgtca
     8401 cccggctatt tcgccggacc ggtcgccccg cacacctcgc tcctcgccct cgcgacctcg
     8461 ctcggccaca cggtcctcgc ggtggaacgg cccggctacg gtctctcccg cgccgccctc
     8521 ccgcacggtc tgccgctcgc cgaacaggcc ctgctgctgc gccgatcgct cgccgactac
     8581 gccggactcc acccggtcgg ggccgggttc ttcctgctcg gacactccga cggcggcaag
     8641 accgccctgc acctggcagg ggagccctgg accggagcga cccccgccga ccgcctgctc
     8701 gggctcgacc tcaacggctg cggctggcac tacgcaccgg ccgccgccca cttcccggac
     8761 acgctggccg gcggggcggg agagctcaac tgggggccgc tgcgcctcta tccgggcggc
     8821 accttccggg ccagccgtgc cctcctccgt gaggttccgg ccgcggagga ggcggagacg
     8881 gggcggtggc ccgcccggtt cccggcggcc gccgcccggg tgcgctgccc ggtacgcctc
     8941 accttcggcg cgtacgaggg ctggtggcgc ctggaccggg acgaactggc cgcggtggcc
     9001 gcgagcttca cgggcacgcg ccgcccggcc gtcgaacgcc tgccggaggc cggacacaac
     9061 ctcagcctcg ggctggccgc cccgctctac cacgcacggg cgctggcgtt cctggaggag
     9121 tgcctggcgg cctcctccgg cggccggggg tgacgtacgc gtatgcgcgt gagtgcgtga
     9181 ggtatggacc tttgggccct gaacctgttg ggatgatcgc gcgcaccgcg tacacgctcc
     9241 gttctgccgc gtgcaccgta cgccaccgcc cgggagagtc catccgatcg acaggactgc
     9301 gacatgagcc attaccctcc ccatgccacc ccgtaccagg ccggcccggt ccggcccggc
     9361 gggcatccgc ccgccggtga ccggatcacc gcgccgctcc tcgtggcatc cgtgctctgc
     9421 acggggctga tggcggggct gttcttcgcc tacgacatct cggtgatgcc gggcctggcc
     9481 gagctggacg acgccgccta cgcggcggcc atgcagcggt tcaacgcggc gatcgacggc
     9541 agcgcgctgt tcggcctggt cttcctcgcc acgctggggc tcacggtcgc cgccgcgatc
     9601 gtggccttcc gccgcaagcg ccgcgcggtg gccctgccgc tggccgccgc ggcactctgc
     9661 tatctgctgg tgctcgtcgt caccgtcgcg gtcaaccttc ccctcaacgc ggacctcgcg
     9721 gcgctcggcg gctccgcgtc cgcccaggac gtgcacgcgg tgatccacga cttcaaatcg
     9781 gtctgggtgc cggtgaacat cttccggacc cttttctgtg ttctgagtct cggtgctctg
     9841 tgcgccgctc tgctccggta cggaaagccg gaaacccctg tgctccggta gggaaaagcc
     9901 cggggaggca attccccggg cttcgttcca tatcggcacg cccctgcctt ccgtattctc
     9961 gcgtgcccat ccttcatttg cgttcgcata gggcgagccc tctttctgcg ccggtcggcg
    10021 aaagggggcc acctgtgtgt gccgtgctcc gcctccgtac cgcccgacgc ggtgcggccg
    10081 ctctccggcc cccgccggtc ggcggcccgg agagatgtgg gagcgtggga acggtgagtg
    10141 acaatccgac ggaatccatg cagtaccgct tcgacgggcc cgaggacgcc ccggtcctgg
    10201 tgatcggccc ctcgctcggg acgacgtggc acatgtggga tcgccagatt cccgagctgt
    10261 cccagcactg gcggatcttc cgctacgacc tgcccggcca cggcggcgcg cccgcccacg
    10321 ccgccgcctc cgtcgccgag ctgggcgacc ggctgctcgc gaccctggac ggtct
//
