LOCUS       NZ_CP079114            11350 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. WY228 chromosome.
ACCESSION   NZ_CP079114
VERSION     NZ_CP079114.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WY228
  ORGANISM  Streptomyces sp. WY228
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8286721)
  AUTHORS   Qin,S.
  TITLE     Volatile organic compounds produced by endophyte Streptomyces sp.
            WY228 mediate plant growth and salt stress tolerance by activating
            hormonal signaling and anthocyanin biosynthesis,
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8286721)
  AUTHORS   Qin,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2021) Key lab of biotechnology of medicinal
            plants, Jiangsu Normal University, No. 101 Shanghai Road, Xuzhou
            221116, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019287315.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 06:02:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,303
            CDSs (total)                      :: 7,213
            Genes (coding)                    :: 7,059
            CDSs (with protein)               :: 7,059
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 83 of 155
            Pseudo Genes (incomplete)         :: 99 of 155
            Pseudo Genes (internal stop)      :: 10 of 155
            Pseudo Genes (multiple problems)  :: 36 of 155
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:35:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6752549
            Orig. end                         :: 6763899
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079114.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11350
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="27"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11350
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11350
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6350](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6350)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    386..401
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.74"
                     /tool="antismash"
     misc_feature    487..496
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    complement(937..953)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.3"
                     /tool="antismash"
     gene            1364..2092
                     /gene="hemQ"
                     /locus_tag="KV381_RS29550"
                     /old_locus_tag="KV381_29550"
     CDS             1364..2092
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="KV381_RS29550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_29550"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_030079714.1"
                     /transl_table=11
                     /translation="MSAPETVTSSKSPNAGKKAKDLNEVIRYTLWSVFKLRDVLPLDRA
                     GYADEVQELFDQLAAKDVTVRGTYDLSGLRADADIMIWWHAETSDQLQDAYNLFRRTKL
                     GRALDPVWSNMALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARLHVRE
                     EVPFYTGRRKSVADLVAGLA"
     gene            complement(2241..3803)
                     /locus_tag="KV381_RS29555"
                     /old_locus_tag="KV381_29555"
     CDS             complement(2241..3803)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (102-476). E-value:
                     4.6e-19. Score: 60.9. Matches aSDomain:
                     nrpspksdomains_KV381_RS29555_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956907.1"
                     /locus_tag="KV381_RS29555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_29555"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_218786730.1"
                     /sec_met_domain="PF00561 (E-value: 1.8e-24, bitscore: 85.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTAALYTSIGSLVLSALAAAPANAWEAPGSAESRGTAIAAARAT
                     AAGIDFAACPEEEMLPDSLKCGTVKVPLDYAEPDGRQLELTVSRTPATGPAQERQGSFV
                     YNPGGPGASSITFPLAGELPEWKEIAEAYDLVGYAPRGVNGSSAPLSCQDPAAYTKGPT
                     DAPTHPTQAYKERRVARAKAYARGCAEHAGATLRHYTSLNNARDLDVLRAALGESRLTF
                     MGASYGTYFGALYATLFPSHVRRMVFDSVVDPDPEQIWYRSNMDQSLAFETRWEDFRRW
                     VARHDDVYHLGATPEAVQRRYDEVRAELAVKPAGGKVGPGQLHSAFLGAGYYDDYWAMR
                     ATALSEYVRGNPEPLVAQASPRAVAAKDNENAQAVYTAVECNDAPWPEEFEIWDRDHTD
                     LARVAPFETWDNAFTNLPCAFWPAPRQRPLEVGTRWGELPPVLILAAERDAATPYKGAL
                     ELRKRLPGSSLVTERGAGTHGVGGAGNTCVDDHLRRYLLTGEVPGRGADCAAHPEPNPV
                     SLD"
     aSDomain        complement(2376..3497)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KV381_RS29555_Abhydrolase_1.1"
                     /evalue="4.60E-19"
                     /label="KV381_RS29555_Abhydrolase_1.1"
                     /locus_tag="KV381_RS29555"
                     /protein_end="476"
                     /protein_start="102"
                     /score="60.9"
                     /tool="antismash"
                     /translation="FVYNPGGPGASSITFPLAGELPEWKEIAEAYDLVGYAPRGVNGSS
                     APLSCQDPAAYTKGPTDAPTHPTQAYKERRVARAKAYARGCAEHAGATLRHYTSLNNAR
                     DLDVLRAALGESRLTFMGASYGTYFGALYATLFPSHVRRMVFDSVVDPDPEQIWYRSNM
                     DQSLAFETRWEDFRRWVARHDDVYHLGATPEAVQRRYDEVRAELAVKPAGGKVGPGQLH
                     SAFLGAGYYDDYWAMRATALSEYVRGNPEPLVAQASPRAVAAKDNENAQAVYTAVECND
                     APWPEEFEIWDRDHTDLARVAPFETWDNAFTNLPCAFWPAPRQRPLEVGTRWGELPPVL
                     ILAAERDAATPYKGALELRKRLPGSSLVTERGAG"
     gene            complement(3952..4995)
                     /locus_tag="KV381_RS29560"
                     /old_locus_tag="KV381_29560"
     CDS             complement(3952..4995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514514.1"
                     /locus_tag="KV381_RS29560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_29560"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_218786731.1"
                     /transl_table=11
                     /translation="MNVFAVLATLGVVVSSVLLVVKAVAERSRRPAPGPGVALHDLYEV
                     AFLNGGPARVVDTALASLHADGRLAVGGPGIVAVQRAQANDPVERAVFQELAAAPSGAL
                     HVLREAVMRHPAVQEVGDGLAARGLLVPPHRIRPLRRWGLIQGVACLIALPLSLVVTFV
                     QLAVVDTMDFPVPFIVKILPAIVIGAFSGLIVAGASASRITKSGQHAALAFRTAYAHVV
                     TPAHLVATLGLRALPDPVLQAQLVAAARLSAPRRRSSSYVAGSTAAGVTAVGVATVWCA
                     AAGPGGSSCGGSTGGGSGSSCGGGSGCSSGSGCSSGSGCGGSSGSSCGSSGGSSCGGGG
                     GGCGGGS"
     gene            complement(5001..6350)
                     /locus_tag="KV381_RS29565"
                     /old_locus_tag="KV381_29565"
     CDS             complement(5001..6350)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059402.1"
                     /locus_tag="KV381_RS29565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_29565"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_218786732.1"
                     /sec_met_domain="DUF692 (E-value: 6e-90, bitscore: 299.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLGIGIGWRPEIADEVEALAGIDWVEAVAENLCADHLPDSLVRL
                     RERGVTVVPHGVALGLGGADRPDPGRLAALAARAELLGTPLVTEHIAFVRAGGPLTASP
                     RLEAGHLLPVARTWDALDVLCENVRIAQDSLPVPLALENIAALISWPGEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNLGQDPAEALDELPVEAIAYVHVAGGVEKNGVWHDTHA
                     HPVSAPVLEVLAELRSRVDPPGVLLERDDAFPPGTELAGELDAIRATLRQAATVHTPAE
                     SGERTDTAESGERTGVADPAPAEGRAAADPVPAGTRDRTAVAQTALLSALVVGTPAPEG
                     FDHRRLRVQSRALAAKRADVVAKVAPELPEILGDGYRPAFLAYAGSRPMSGGYRRDALD
                     FAEHVLIAGGPADPVARRRLTYWWQDRSGARPPGRTTRLVRAARAVLVGK"
     gene            complement(6481..7227)
                     /locus_tag="KV381_RS29570"
                     /old_locus_tag="KV381_29570"
     CDS             complement(6481..7227)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072530.1"
                     /locus_tag="KV381_RS29570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_29570"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_030631816.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVATIGALAFPVWSYADRSGTGEANLNASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPSEATKAAGDHLVVGHADLDRRAR
                     DVAAKLGVELPNQPTAQQQEWLRELTAASGDEYERKFANILRAAHGKVFGLIGQVRHTT
                     RNTLIRQLASDANQTVLDHITMLEATGFVDFDALAREAASGSTASPSGPSMPRDGQVPP
                     APVPATPTGDQSFTSRPVPPTMMPMP"
     gene            complement(7446..8168)
                     /locus_tag="KV381_RS29575"
                     /old_locus_tag="KV381_29575"
     CDS             complement(7446..8168)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072531.1"
                     /locus_tag="KV381_RS29575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_29575"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_218786733.1"
                     /transl_table=11
                     /translation="MTSNDTPVSESPFHNGPTDRDQAPQFVLPLVLHLEKTDPPARTDA
                     LRTAARAVLTILSDERSLGDGEWAAAMRDWQDARIRKVVRRARGAEWRKATALDGITVT
                     GEDGAEVRVFPPIPLDGWPKELAKLQVSGTDLEEPEPPAAPDLSGPVLWLNPEVDMSAG
                     KTMAQAGHGAQLAWWELSDAERKAWREAGFPLSVATPGPERWRELTVSGLPVVRDAGFT
                     EIAPGSCTVVADHPALRR"
     misc_feature    complement(8128..8143)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.4"
                     /tool="antismash"
     gene            8239..8913
                     /locus_tag="KV381_RS29580"
                     /old_locus_tag="KV381_29580"
     CDS             8239..8913
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378552.1"
                     /locus_tag="KV381_RS29580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_29580"
                     /product="AIM24 family protein"
                     /protein_id="WP_030079702.1"
                     /transl_table=11
                     /translation="MKSDLFSSEHMAQPATAPGMTLQNSKSIKYAVNGEMHARQGSMIA
                     FRGNLQFERKGQGIGGLLKRAVTGEGLALMAVRGQGEAWFAHEAANCFIVELDQGDSIT
                     INGRNVLCFDPTLSYEIKTVKGAGMVGGGLFNSVFTGYGKLGLMCEGTPIVIPVTAAQP
                     VYVDTDAVVGWSQQLATSLNRSQSVGSMVRGGSGEAVQLMLQGEGFVIVRPSEARPEKT
                     SN"
     gene            8916..9935
                     /locus_tag="KV381_RS29585"
                     /old_locus_tag="KV381_29585"
     CDS             8916..9935
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317912.1"
                     /locus_tag="KV381_RS29585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_29585"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_218787322.1"
                     /transl_table=11
                     /translation="MHLRGVGHRYGLRGAWVLRGVDLALPARALVRVEGANGVGKSTLL
                     RLLAGIDAPAEGRITGRPARTAYVPERFPAALPLTATGYLVHLGRIHGLRTAEAKRRAG
                     EWLNRFGAADHARTPLTELSKGTSQKVAVAQALLAAPDLLVLDEAWTGLDTAARAELDR
                     AVAERVAAGATVVFVDHDPRRLAGAADLTLRTADGGVHRATAATATGPDPGPRVLIEAV
                     GPPGATLPEALPGDPVRERRSGTGGFGAGAAVSVPGGGTADYSPGPGPGDGPDHGAGSA
                     VVRLTVPAPYSDALLAALLTARPPWHIRQVAPAQAPAPVTSPESPESSEAAAEVSEAS"
     gene            9932..10594
                     /locus_tag="KV381_RS29590"
                     /old_locus_tag="KV381_29590"
     CDS             9932..10594
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452202.1"
                     /locus_tag="KV381_RS29590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_29590"
                     /product="hypothetical protein"
                     /protein_id="WP_030079700.1"
                     /transl_table=11
                     /translation="MTALLRYQTAVLARSQRWLAPLLLYAAVLAVGVQGGQPVLGSLGV
                     AALLPVTAWAVHLCVTQEPPAARTVASAAVGPRRAHLAALLTATGCAAALAVVSTAVVL
                     LVSSPATDDGAHAVSRPSAALAGLAAALCCVLLGAVVGALSSRPVTVRRGWSVAAAVLG
                     SLLALVTTGSPARYAVTALVTGSRTGTFQPPALALLGAAALAGAVALAVCRLAARRG"
     gene            10604..10837
                     /locus_tag="KV381_RS29595"
                     /old_locus_tag="KV381_29595"
     CDS             10604..10837
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491669.1"
                     /locus_tag="KV381_RS29595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_29595"
                     /product="hypothetical protein"
                     /protein_id="WP_050495553.1"
                     /transl_table=11
                     /translation="MRRLVDVDTDDTHDTNGSYCEAPGPIAPAPPAGAAEGPPYAECVL
                     CREPTEYPESTKGATLCPVCAWQEAGRTACSG"
ORIGIN
        1 gtgcgggtca ccctcctgga ggccacggac cggctcggcg gcaagctcat gaccggcgag
       61 gtcgcgggcg tccaggtcga cctgggggcc gagtcgatgc tcgcccggcg ccccgaggcg
      121 gtcgccctgg ccgaggcggt gggcctcgcc gaccgcctcc agccgcccgc caccgccacc
      181 gcctcgctct ggacccgcga cgcgctgcgc cccatgccca agggccatgt catgggcgtc
      241 cccggcgacc ccgcggtcct cggcgaggtg ctgtcacccg agggcctggc ccggatcgcc
      301 gaggagcgcg acctcacccc gacgcccgtg ggcgaggacg tcgcggtcgg cgcgtacgtc
      361 gccgaccggc tcggccgcga ggtcgtggac cggctggtgg agcccctcct cggcggggtc
      421 tacgcgggcg acgcctaccg gatctcgatg cgcgccgccg taccccagct cttcgaagcg
      481 gtgaaggaag gcggcccgct gctggaggcc gtgggccgca tccaggaacg cgcggcggcc
      541 cggcagcagg ccggcccggt cttccagggc atcgagggcg gcatcggcac cctcccggcc
      601 gccgtcggcg acgccgtacg ggccgggggc ggcgacatca tcaccgagac gcccgtcctc
      661 ggtctcaccc gtacggaaac gggctgggcc gtccgcaccg acacccggac gatcgccgcc
      721 gacggcatcg tcctggccac ccccgcctgg tccgccggca ccctgctggc cgccgagtcc
      781 ccggccgcct ccgccgagct gtccggcgtc gagtacgcct cgatggccct ggtcaccctg
      841 gccttccggc gtaccgacat cgaggcgtac tcggccctgc gcggccgctc cggcttcctc
      901 gtaccgccgg tcgacgggca caccatcaag gcgtccacgt tctccagcaa caaatggcag
      961 tgggtcgccg acgccgcccc cggcctcttc gtgctccgca cctccgtcgg ccggtacggc
     1021 gaggaggacc acctccaccg cgaggacgag gaactggtct ccgtgtccct gcgcgacctc
     1081 gccgcggcca ccggactcac cgcccggccc gtcgacagcg aggtcacccg ctggatcggc
     1141 ggactgccgc agtacccggt cggccacctc acccgggtcg cccggatccg cgacgaggtc
     1201 gccaagctgc ccgccctgcg ggtctgcggc gcggtctacg acggcgtcgg catccccgcc
     1261 tgcatcgcga gcgcccaccg ggcggccgac gagatcacca gggagatcat cgccacgccg
     1321 accctggttc ggggcacggg gagcgaggcg ggacaatagc cgtatgagtg ctcctgagac
     1381 tgtgacatca agcaagagtc cgaacgccgg caagaaggcc aaggacctca acgaggtcat
     1441 ccgctacacc ctgtggtcgg tcttcaaact gcgcgacgtc ctcccgctgg accgcgccgg
     1501 ttacgccgac gaggtccagg agctgttcga ccagctcgcg gccaaggacg tcaccgtccg
     1561 gggcacctac gacctctccg ggctgcgggc cgacgcggac atcatgatct ggtggcacgc
     1621 ggagacctcg gaccagctcc aggacgcgta caacctcttc aggcgcacca agctcggccg
     1681 cgcgctcgac ccggtctggt cgaacatggc gctgcaccgc cccgccgagt tcaacaagtc
     1741 gcacatcccg gcgttcctcg ccgacgagac gccccgcgac tacatcagcg tctacccctt
     1801 cgtccggtcc tacgactggt atctgctgcc cgacgaggac cgccgccgca tgctcgcgga
     1861 ccacggcaag atggcccggg gctacccgga cgtccgcgcc aacaccgtcg cctcgttctc
     1921 gctcggcgac tacgagtgga tcctcgcctt cgaggcggac gagctgtacc gcatcgtcga
     1981 cctgatgcgt cacctgcgcg cctccgaggc acggctccac gtgcgcgagg aggtcccgtt
     2041 ctacacgggc cgcaggaaga gcgtcgcaga cctggtggcc gggctcgcat aaccagcgag
     2101 tcccgcacca ccccaacccg gaagatcaga gggacgggca ccgggcaccg atcgtgccgt
     2161 ccgcccctcc agcgacaccg ggttcggctt cgggggccgt cgcaccgtga aggtgcggcg
     2221 gcccccgctc cgtactcggc tcagtccagc gacaccgggt tcggctccgg gtgcgcggca
     2281 cagtccgcac cccgccccgg cacctcaccg gtcagcagat accggcgcag atgatcgtcc
     2341 acgcaggtgt tcccggcccc gccgacgccg tgcgtccccg cgccccgctc ggtcaccagc
     2401 gacgacccgg gcagccgctt ccgcagctcc agcgccccct tgtacggcgt cgccgcgtcc
     2461 cgctccgccg ccaggatcag caccggcggc agctcgcccc accgcgtccc cacctccagc
     2521 ggccgctgcc ggggcgcggg ccagaatgcg cacggcaggt tggtgaaggc gttgtcccag
     2581 gtctcgaagg gagcgacccg cgccaggtcc gtgtggtcgc ggtcccagat ctcgaactcc
     2641 tccggccagg gcgcgtcgtt gcactcgacc gccgtgtaca ccgcctgcgc gttctcgttg
     2701 tccttcgccg ccaccgcacg cggggaggcc tgtgcgacca gcggctccgg gttgccccgc
     2761 acgtactccg acagggccgt cgcccgcatc gcccagtagt cgtcgtagta accggccccc
     2821 aggaacgccg aatgcagctg ccccggcccg accttgccgc ccgcgggctt caccgccagc
     2881 tcggcgcgca cctcgtcgta gcgccgctgc accgcctccg gggtggcccc caggtggtac
     2941 acgtcgtcgt gccgggccac ccagcggcgg aagtcctccc agcgcgtctc gaaggccagc
     3001 gactggtcca tgttggagcg gtaccagatc tgctccgggt ccgggtcgac caccgagtcg
     3061 aagaccatcc gccgtacgtg cgaggggaac agcgtcgcgt acagcgcgcc gaagtacgtc
     3121 ccgtacgacg cccccatgaa cgtcagccgg ctctcgccga gcgcggcccg caggacgtcc
     3181 aggtcacggg cgttgttcag cgaggtgtag tgccgcagcg tcgcgcccgc gtgctccgcg
     3241 cagccccgcg cgtacgcctt cgcacgggcg acgcgccgct ccttgtacgc ctgcgtcggg
     3301 tgcgtcggcg cgtccgtcgg acccttggtg tacgcggcgg ggtcctggca ggacagcggg
     3361 gcggaggagc cgttcacccc gcgcggggcg tagccgacca ggtcgtacgc ctcggcgatc
     3421 tccttccact ccggcagctc cccggccagc gggaaggtga tgctggacgc gccggggccg
     3481 cccgggttgt agacgaacga gccctgccgc tcctgcgccg gaccggtggc gggcgtgcgg
     3541 ctgacggtca gttcgagctg ccgcccgtcc ggctcggcgt agtcgagggg caccttcacc
     3601 gtgccgcact tcagggagtc cgggagcatc tcctcctcgg ggcaggcggc gaagtcgatc
     3661 ccggcggcgg tggcccgggc ggcggcgatc gcggtgcccc gggactcggc ggagcccggc
     3721 gcctcccagg cgttcgcggg agcggcggcc agggcggaca ggaccaggga gccgatcgat
     3781 gtgtacagcg cagctgttct cacgcgcggg gcctctctgc ggatgcggag agggatggtc
     3841 gggcggacgg gcgccgatgt ccagtaccgc cccggaatgt caccgggaac caccctgatg
     3901 ggcggtcaac aaccgtacga gtggcctcgg ttcgcccgga tcaggagcgg ttcaggaccc
     3961 gccgccgcag ccgccgccac cgcccccgca gctggagccg cccgagctgc cgcagctcga
     4021 accgctgctg cctccgcacc cggacccgga ggagcatccg gaccccgagg agcatccgga
     4081 cccgccgccg cagctcgatc cggagccgcc cccggtcgac ccgccgcagc tggagccgcc
     4141 ggggccggcg gcggcgcacc agaccgtcgc gacgccgacg gcggtgacgc ccgccgccgt
     4201 ggacccggcg acgtacgacg aggaccgccg gcgcggagcg ctcagccgtg ccgcggccac
     4261 cagctgggcc tggagcaccg ggtccggcag cgcgcggagc cccagcgtgg cgacgaggtg
     4321 ggcgggcgtc accacatggg cgtaggcggt ccggaacgcc agggcggcgt gctgccccga
     4381 cttcgtgatg cggctcgcgg aggcgcccgc gacgatcagg ccgctgaacg cgccgatcac
     4441 gatcgcgggc aggatcttca cgatgaacgg gaccgggaag tccatggtgt cgaccaccgc
     4501 gagctggacg aacgtgacca ccaggctcaa cggcagggcg atgaggcacg ccaccccctg
     4561 gatcagcccc caccgccgca gcggccggat ccggtggggc ggcaccagca gcccgcgagc
     4621 ggcgagcccg tcgccgacct cctggaccgc cgggtggcgc atcaccgctt cccgcaggac
     4681 gtgcagggcg ccgctcgggg cggcggccag ctcctggaac accgcgcgtt ccaccgggtc
     4741 gttcgcctgc gcccgctgta cggcgacgat gccgggcccg ccgacggcca gccggccgtc
     4801 cgcgtgcagc gaggcgagag cggtgtccac caccctggcc ggaccgccgt tgaggaacgc
     4861 gacctcgtac aggtcgtgaa gggcgacgcc gggccccggg gcggggcggc gggaccgctc
     4921 ggccaccgcc ttcacgacga gcagcaccga cgagacgacg accccgaggg tcgccagcac
     4981 agcgaagacg ttcacagcgg tcacttcccc acgaggacgg cacgggccgc ccggaccaga
     5041 cgggtggtgc ggcccggcgg ccgggcgccc gaccggtcct gccaccagta cgtcagccgg
     5101 cgccgcgcca ccggatcggc gggaccgccc gcgatgagca catgctccgc gaagtccagg
     5161 gcgtcgcggc ggtacccacc ggacatcggg cggctgccgg cgtacgcgag gaacgcgggg
     5221 cggtagccgt cgccgaggat ctccggcagc tcgggggcca ccttggcgac gacgtcggcg
     5281 cgcttggcgg ccagcgcccg gctctgcacc cgcagccgcc ggtggtcgaa gccctcgggg
     5341 gccggggtcc cgaccaccag ggcggagagc agcgcggtct gcgcgacggc ggtacggtca
     5401 cgggtgccgg ccggcacggg gtcggccgcg gcgcggcctt cggcgggcgc ggggtcggcg
     5461 acgcccgtac gctcaccgga ttcggcggtg tccgtacgtt caccggattc ggccggcgtg
     5521 tgaacggtcg ccgcctgccg cagcgtcgcc cggatcgcgt ccagttcgcc cgccagctcc
     5581 gtgcccggcg ggaacgcgtc gtcccgctcc agcagcacgc ccggcgggtc gacccgggag
     5641 cgcagctcgg ccagcacctc caggacgggt gcggacaccg ggtgggcgtg cgtgtcgtgc
     5701 cagacgccgt tcttctcgac cccgcccgcc acatgcacgt acgcgatggc ctccaccggc
     5761 agctcgtcca gtgcctccgc cgggtcctgg ccgaggttga cgtggttggt gtgcagattg
     5821 gccacgtcga tcagcagccg caccccggtc cgctcgacca gttccgcgag gaactgcccc
     5881 tcggtcagct cctcgcccgg ccaggagatc agcgccgcga tgttctccag ggcgagcggc
     5941 accggcagcg agtcctgcgc gatccgcacg ttctcgcaca gcacgtccag cgcgtcccag
     6001 gtacgcgcca ccggcagcag gtgccccgcc tccagcctcg gtgacgcggt cagcggcccc
     6061 ccggcccgta cgaacgcgat gtgctcggtc accagcggcg tccccagcag ttcggcgcgc
     6121 gccgcgagag cggcgaggcg gccggggtcg ggccggtccg caccgccgag cccgagcgcc
     6181 accccgtgcg gcacgacggt caccccgcgc tcccggagcc gtaccaggga atccgggaga
     6241 tggtcggcgc agaggttctc cgcgacggcc tcgacccagt cgatccccgc cagcgcctcc
     6301 acctcgtccg cgatctccgg ccgccagccg ataccgatgc ccagcttcat ggtgtccccc
     6361 tgctcaccca ggccttcgct ctgtcgttct gtccaggggt catgaccccg ccgaacggtg
     6421 ctgaacccga cagaggggac gttcagagct ggatttgagg ttccgggacc ggcggcctcg
     6481 ttatggcatc ggcatcatcg tgggcggcac cggccgcgag gtgaaggact ggtcgccggt
     6541 cggcgtcgcc ggcaccgggg ccggcgggac ctggccgtcc cggggcatcg acgggccgga
     6601 gggactggcg gtcgacccgc tcgccgcttc gcgcgccagg gcgtcgaagt cgacgaaccc
     6661 ggtggcctcc agcatggtga tgtggtccag cacggtctgg ttggcgtcgc tcgccagctg
     6721 ccggatcagc gtgttgcggg tggtgtggcg gacctggccg atcaggccga agaccttgcc
     6781 gtgggcggcg cgcaggatgt tggcgaactt ccgctcgtac tcgtccccgc tcgccgccgt
     6841 cagctcccgc agccactcct gctgctgggc ggtcggctgg ttgggcagct ccaccccgag
     6901 cttcgccgcc acgtcccggg cccgccggtc cagatcggcg tgcccgacga cgaggtggtc
     6961 cccggcggcc ttcgtcgcct cactgggcgc ccgttcgatc gcctgctgcc cggcgggcag
     7021 ctcccacagc ccggcgagcc gcaccttgac caggaagtcc cggtcggtcg cggagagcgg
     7081 tccccactgg gtcgcgacac tggaggcgtt gaggttcgcc tcgccggtgc ccgaacggtc
     7141 cgcgtaggac cagacgggga acgcgagcgc gccgatggtg gcgaccagcg ccgcgatgat
     7201 gagggccgtt ccgttgatgc gtcgcaacaa ggtgtctccc ggggccgagg cgtggcagcg
     7261 tcgatcgaac aggcgtcaat caaccgccag tcgaccggct gcgcaggcta cttggcggta
     7321 atgccgaaag ggcagtaccg ggagatacgg agaagggacc ggaaacgttc agcacggcgg
     7381 cgatgtggtc gtgtcccgct cggcggctcc gcgacacggg cgatccgtcg cggtcaccgc
     7441 cccgctcagc ggcgcagcgc cggatggtcg gcgaccaccg tgcaggagcc cggggcgatc
     7501 tcggtgaacc ccgcgtcgcg caccaccggc agcccgctca ccgtcagctc ccgccagcgc
     7561 tccgggccgg gggtggcgac ggagagcggg aacccggcct cccgccaggc cttgcgctcg
     7621 gcgtcggaca gctcccacca ggcgagctgc gccccgtgcc cggcctgggc catcgtcttg
     7681 cccgccgaca tgtccacctc ggggttcagc cacagcaccg ggcccgagag atcgggcgcg
     7741 gcgggcggct cgggttcctc caggtcggtg cccgacacct ggagcttggc cagctccttc
     7801 ggccagccgt ccagcgggat cggcgggaac acccgtacct cagcgccgtc ctcgcccgtc
     7861 accgtgatgc cgtccagcgc cgtggccttg cgccactccg caccgcgcgc ccggcgcacg
     7921 accttgcgga tccgggcgtc ctgccagtcc cgcatcgccg cggcccactc gccgtcgccg
     7981 agcgaccgct cgtcggagag gatcgtcagc accgcacggg cggcggtccg cagcgcgtcg
     8041 gtgcgggcgg gcgggtccgt cttctccagg tggagcacca gcggcagcac gaactgcggc
     8101 gcctggtcgc ggtcggtcgg accgttgtgg aacgggcttt cggagacagg ggtgtcgttg
     8161 ctggtcaccg ccccagtctg ccaggtggtc ggttcacctc cttggcggaa cgaccggctc
     8221 cgggtgagga tgctccgcat gaagagcgat ctcttttcca gcgagcacat ggcccagccg
     8281 gccaccgccc ccggtatgac cctgcagaac tccaagtcga tcaagtacgc cgtcaacggg
     8341 gagatgcacg cccggcaggg ttcgatgatc gccttccgcg gcaacctcca gttcgagcgc
     8401 aagggccagg gcatcggcgg cctgctcaag cgcgcggtca ccggtgaggg cctcgcgctg
     8461 atggcggtcc ggggccaggg cgaggcgtgg ttcgcgcacg aggcggcgaa ctgtttcatc
     8521 gtggagctgg accagggcga ctccatcacc atcaacggcc gcaacgtcct gtgtttcgac
     8581 ccgacgctct cctacgagat caagacggtg aagggcgccg gaatggtcgg cggcggcctc
     8641 ttcaacagcg tcttcaccgg ttacggcaag ctcggcctga tgtgcgaggg cacgccgatc
     8701 gtgatccccg tgacggccgc tcagccggtg tacgtggaca cggacgcggt cgtcggctgg
     8761 agccagcagc tcgcgaccag cctcaaccgc tcccagagcg tcggctcgat ggtgcgcggc
     8821 ggctccggcg aggcggttca gctgatgctc cagggcgagg gcttcgtcat cgtgcggccc
     8881 agcgaggccc ggcccgagaa gacctccaac tgaccctgca tctccggggc gtgggccacc
     8941 ggtacggcct ccgcggcgcc tgggtgctgc gcggggtcga cctcgcgctg cccgcgcggg
     9001 cgctcgtccg ggtcgagggg gccaacgggg tcggcaagtc gaccctgctg cggctgctcg
     9061 ccgggatcga cgcccccgcc gagggacgga tcaccggccg cccggcccgt acggcatacg
     9121 ttcccgagcg cttcccggcc gcgctgccgc tgaccgccac cggctacctc gtccacctcg
     9181 gccggatcca tgggctgcgc acggccgagg cgaagcggcg ggcgggggag tggctgaacc
     9241 ggttcggcgc ggcggatcac gcccgtaccc cgctgacgga actctccaag ggcaccagcc
     9301 agaaggtcgc cgtcgcccag gcgctgctgg ccgcgccgga cctgctggtt ctggacgagg
     9361 cctggaccgg cctggacacg gcggcccgcg ccgagctgga ccgcgccgtg gccgaacgcg
     9421 tggcggccgg cgccaccgtc gtcttcgtcg accacgaccc gcgacgcctg gccggggcgg
     9481 cggacctcac cctgcgaacc gccgacggcg gggtgcaccg ggcgacggcc gcgacagcga
     9541 ccggccccga ccccggcccc cgggttctga tcgaggcggt gggcccaccg ggcgcgacgc
     9601 tgcccgaggc cctgccgggc gacccggtcc gggagcggag gagcgggacg ggcggcttcg
     9661 gcgcgggtgc ggccgtctcc gtcccgggcg gcggcaccgc tgactacagc cccggccccg
     9721 gcccgggtga cggacccgac cacggcgcgg gctccgccgt cgtacgcctc accgtccccg
     9781 ccccgtactc cgacgcgctc ctggcagccc tgctcacggc ccgcccgccg tggcacatcc
     9841 gacaggtcgc cccggcacaa gcccccgcac cggtcacgtc acccgagtca cccgagtcct
     9901 ccgaggcagc ggcagaggtg tccgaagcat catgaccgcc ctcctccgct accagaccgc
     9961 cgtgctcgcc cgctcccagc gctggctggc gcccctcctg ctgtacgcgg ccgtcctcgc
    10021 cgtcggcgtg cagggcgggc agcccgtgct gggctcgctc ggcgtcgccg ccctgctgcc
    10081 cgtgaccgcg tgggccgtcc acctctgcgt cacccaggag ccgcccgccg cccggacggt
    10141 cgcctcggcc gccgtgggcc cccggcgggc gcacctggcc gcgctgctca ccgccacggg
    10201 ctgcgcggcg gcgctcgccg tggtgagcac cgccgtcgtc ctgctcgtca gcagcccggc
    10261 caccgacgac ggcgcccacg cggtctcccg accgtccgcc gcgctcgccg gactggcggc
    10321 cgccctgtgc tgcgtcctgc tgggcgcggt cgtgggggcg ctcagcagcc gcccggtgac
    10381 ggtccgccgg ggctggtccg tggcggcggc ggtgctcggc tcgctcctgg cgctggtgac
    10441 caccggatcc ccggcgcgtt acgcggtcac cgccctggtc acgggatcac ggaccgggac
    10501 gttccagccg cccgccctgg ccctgctcgg ggcggcggct ctcgccgggg cggtggcgct
    10561 cgcggtctgc cggctcgccg cccggcgcgg atgagccggg cggatgcgta ggctcgtgga
    10621 cgtggacacc gacgacacgc acgacacgaa cggttcgtac tgcgaggccc cgggaccgat
    10681 cgcgcccgcc ccgcccgccg gagccgccga gggccctccc tacgccgagt gcgtgctgtg
    10741 ccgcgagccg accgagtacc cggagtcgac gaagggcgcc acgctctgcc cggtctgcgc
    10801 ctggcaggag gcgggccgca cggcctgttc cggctgaggt gcacagtggg gagtctgccg
    10861 cgcggggtgc gcgggccggg cttcctgcag gacgtccggc ggtcgtgccc gtcggctcag
    10921 gctgtgggcc tgggacggtt caggtcacgc cagtcccggg gcgagatgcc gtaagcctgt
    10981 ttgaacacct tgctgaagtg gctcgcgtcc acgaagcccc agcgtcgccc tgtcgccgcg
    11041 atggtgcgca ggtggccgtg cggaccggca agctcccgtc ggcattcggc caggcgatgt
    11101 gaacgcatcc attccccgag tctgatgttc gaccggccca gcacggtgta gagatggcgg
    11161 accgagatgc cgtgcgcggc ggcgatccgc gtggccgaca aatcgggttc agccaggtga
    11221 gcccgcatgt actgggtgat tcgcaggctg agcgtcgact ccaagggtgc cctggccgta
    11281 tcggtgtcgc cgagctgtga tgtcacgacg gcacggatga gttccagact cggttccacg
    11341 accgcgtcgg
//
