LOCUS       NZ_CP079114            10945 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. WY228 chromosome.
ACCESSION   NZ_CP079114
VERSION     NZ_CP079114.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WY228
  ORGANISM  Streptomyces sp. WY228
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8286721)
  AUTHORS   Qin,S.
  TITLE     Volatile organic compounds produced by endophyte Streptomyces sp.
            WY228 mediate plant growth and salt stress tolerance by activating
            hormonal signaling and anthocyanin biosynthesis,
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8286721)
  AUTHORS   Qin,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2021) Key lab of biotechnology of medicinal
            plants, Jiangsu Normal University, No. 101 Shanghai Road, Xuzhou
            221116, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019287315.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 06:02:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,303
            CDSs (total)                      :: 7,213
            Genes (coding)                    :: 7,059
            CDSs (with protein)               :: 7,059
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 83 of 155
            Pseudo Genes (incomplete)         :: 99 of 155
            Pseudo Genes (internal stop)      :: 10 of 155
            Pseudo Genes (multiple problems)  :: 36 of 155
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:35:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 139131
            Orig. end                         :: 150076
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP079114.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10945
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10945
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10945
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5945](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5945)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(429..1469)
                     /locus_tag="KV381_RS00590"
                     /old_locus_tag="KV381_00590"
     CDS             complement(429..1469)
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-269). E-value: 5.5e-13.
                     Score: 40.6. Matches aSDomain:
                     nrpspksdomains_KV381_RS00590_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761872.1"
                     /locus_tag="KV381_RS00590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_00590"
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_030082206.1"
                     /sec_met_domain="ADH_N (E-value: 9.4e-28, bitscore: 95.0,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 4.1e-23, bitscore:
                     80.5, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVAAYAAPRAKAPLERTTIERRPVGEFDILIDIKFAGICHSDI
                     HQARDGWGEGIFPMVPGHEIAGIVAETGSGVTRFKVGDRVGVGCMVDSCGTCDACLMGR
                     EQHCAEGNTQTYNALDRSGEPTYGGYSTHIVVTEKFAVTIPEGIALDEAAPLLCAGITT
                     YSPLKRWGAGPGKKVAVVGLGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHY
                     YATSDPKTFEELAGTFDVILSTVSAPLDFGAYLGLLRTDGTLVNVGAPEEPVSLNLFSL
                     IMGNRSIAGSAIGGIAETQEMLDFCAVHGLGAEIEVIGASQVNEAYERVLASDVRYRFV
                     IDTATI"
     aSDomain        complement(663..1412)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KV381_RS00590_PKS_ER.1"
                     /evalue="5.50E-13"
                     /label="KV381_RS00590_PKS_ER.1"
                     /locus_tag="KV381_RS00590"
                     /protein_end="269"
                     /protein_start="19"
                     /score="40.6"
                     /tool="antismash"
                     /translation="TIERRPVGEFDILIDIKFAGICHSDIHQARDGWGEGIFPMVPGHE
                     IAGIVAETGSGVTRFKVGDRVGVGCMVDSCGTCDACLMGREQHCAEGNTQTYNALDRSG
                     EPTYGGYSTHIVVTEKFAVTIPEGIALDEAAPLLCAGITTYSPLKRWGAGPGKKVAVVG
                     LGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHYYATSDPKTFEELAGTFDVI
                     LSTVSAPLDFGAYLGLLRTDGTLVNVGA"
     gene            1632..2561
                     /locus_tag="KV381_RS00595"
                     /old_locus_tag="KV381_00595"
     CDS             1632..2561
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459219.1"
                     /locus_tag="KV381_RS00595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_00595"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_030082205.1"
                     /transl_table=11
                     /translation="MDDQPEAAAPEPLDRRAELSEFLRTRRAKLQPQDVGLPEFGRARR
                     VPGLRREELAQLAGVSVAYYTRLEQGNGRNVSAEVLDAIARALRLTDAEHAHLNHLARP
                     ARHKKKRRPAKVQRVRTGLLYLLDNMEGIPAYVAGARSDILAWNPMAAAVFGDWAALPP
                     AERNWARLVFLSPAYRDLFLNWDSKASDMVSYLRLYAGCHPDDPQLSALVGELSLKSEE
                     FRRLWATHNVKEKGHGIKLIRHPLVGDLTLSYETLNLPDDEDQQLVTYHAEPGSESAQA
                     LRLLASWGADAGRADVISGAGRGPDDER"
     gene            complement(2524..>3144)
                     /locus_tag="KV381_RS00600"
                     /old_locus_tag="KV381_00600"
                     /pseudo=""
     CDS             complement(2524..>3144)
                     /GO_function="GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA]"
                     /GO_process="GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459221.1"
                     /locus_tag="KV381_RS00600"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_00600"
                     /product="class I SAM-dependent methyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="DIGCGTGVFALLLADRGFQVVGVDPARASVDVARAKPGGDRVRWI
                     SGDASALPPLRVDLVTMTANVAQEITGPRDWRATLRGAYEALRPGGHLVVETRNPARRA
                     WEEWNRERSHRVTEIAGLGPVESWVEVTEVAGPLVSFRWTYVFAADGQVLTSDSTLRFR
                     ERQEAEADLTAQGFVVREVRDAPDRPGREFVLIARRPDPAPPR"
     gene            complement(3148..3876)
                     /locus_tag="KV381_RS00605"
                     /old_locus_tag="KV381_00605"
     CDS             complement(3148..3876)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606639.1"
                     /locus_tag="KV381_RS00605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_00605"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_078846009.1"
                     /transl_table=11
                     /translation="MTKQERATRTRRSLIRSAAVVFEQHGYAQARLTLISSGAGVSTGA
                     LHFHFENKAAVADAVVVEASRVLREMSGAIRRRTDTALQALVDSSHALAERLREDPVSR
                     AGFRLSCDAAGTAAPDLRVQWHHRVRELLDEAAAAGTLADDVSREDAAAATVAATAGFE
                     VLGRHDPGWLSPYTLTGFWQLLLPRLAAAETLPLLDPAGTGTTSSPRIPAPAAGATDEA
                     EAAAGVASGEVASAAVAEAD"
     gene            complement(4144..4989)
                     /locus_tag="KV381_RS00610"
                     /old_locus_tag="KV381_00610"
     CDS             complement(4144..4989)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060637.1"
                     /locus_tag="KV381_RS00610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_00610"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_030629996.1"
                     /transl_table=11
                     /translation="MILVTGATGAVGREVAGQLAAAGPVRILARSPERLTVRGSGVEVV
                     EGEYGDRSALDRAMRGVSSVFLVTKSPTEPHDEWVAAAAAAAGVRHLVKLSMMAVEEPG
                     ADDFITRRQRENEQAVRESGVAWTFVRPRTFMSNTLSWAPGIRSAGVVRALYGDAPVAC
                     VDPRDVAAVAVTALTGTGHEGRAYAVSGPEAITAREQTAELSRVLERPLRFEELGVDAA
                     RTALLARYPRPVAEAFLESAERQRAGAKAAVVPTVEELTGRPARPFRIWSADHADAFSA
                     "
     gene            complement(5001..5945)
                     /locus_tag="KV381_RS00615"
                     /old_locus_tag="KV381_00615"
     CDS             complement(5001..5945)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971211.1"
                     /locus_tag="KV381_RS00615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_00615"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_218784233.1"
                     /sec_met_domain="AfsA (E-value: 1e-24, bitscore: 85.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDAAVLIDPVPAVDSEAEVVHPVGIDMVHRSRPEDAFPRHWVRL
                     ARDRFSVAAVLPHDHPFFAPVSGDRHDPLLVAEAMRQAAMLSFHAGYGVPVGYHFLMAD
                     LDYACDLDHLGVGGAPAEIDVEVFCSDLKWRGGLPVQGRVGWAVHRDGELAATGTGATR
                     FTNPKVYRRMRGDTPAEGISIPRATPVSTAGAGRTRAEDVVLSEAGRDGVWELRVDTRH
                     PTLFQRPNDHVPGMLLLEAARQAACLAVGPGGIVPAEARSRFHRYAEFDSPCSIGAVVR
                     PGAAEDTVTVQVTGHQDGELVFSTVLSGPRANG"
     gene            6102..6740
                     /locus_tag="KV381_RS00620"
                     /old_locus_tag="KV381_00620"
     CDS             6102..6740
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972660.1"
                     /locus_tag="KV381_RS00620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_00620"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_030082200.1"
                     /transl_table=11
                     /translation="MAKQDRAIRTRQTILMAAAQAFEKYGYQAATITEILKSSGVTKGA
                     LYFHFPSKEDLALGVLDAQEPPQTVPDQALKLQELIDLGMLFSHRLQTSLLARAGVRLS
                     MDQQAQGLDRSGPFLRWQEATHHLLTRAKHNGELLPHVNPTETADLYVAAFAGTQAVSQ
                     TLTNYQDLQRRHITLQQHILPSIATPSILTALDLTPERAERLGLLDPVG"
     gene            complement(6802..7413)
                     /locus_tag="KV381_RS00625"
                     /old_locus_tag="KV381_00625"
     CDS             complement(6802..7413)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459226.1"
                     /locus_tag="KV381_RS00625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_00625"
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_218784234.1"
                     /transl_table=11
                     /translation="MMNALTTAAAGFGTGLSLIVAIGAQNALVLRQGVRRDGVLAVVGI
                     CALSDAVLITLGVAGVGAVVVAWPSALTVVGLLGGLFLIGYGLLAARRVLRPSALYAAD
                     APATSRRGVVLTCLAMTWLNPHVYLDTVFLLGSVAADRGPLRWTFGLGAVLASLCWFVA
                     LGYGARLLSGVLARPAAWRVLDALVAVMMIGLGAKLALGA"
     gene            7481..8413
                     /locus_tag="KV381_RS00630"
                     /old_locus_tag="KV381_00630"
     CDS             7481..8413
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971209.1"
                     /locus_tag="KV381_RS00630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_00630"
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="WP_218784235.1"
                     /transl_table=11
                     /translation="MMFELPLDQVRTLLAVVDEGTFDAAAGVLQLTPSAVSQRVKALEQ
                     RTGRVLLMRTKPVRPTESGEVVVRFARQLSRLERDARTALGMAGPDGPTPLSIAVNSDS
                     LATWFLPALRRVPEELDLCYELRREDQDHTAALLREGLVMAAVTSSPEAVTGCSVRGLG
                     RMRYLPVASPAFADRWLERRAGTALHELIGDAPVIGFDRRDDLQDAFVRALGLGPRASA
                     RRHLVPTSEGFVNAVAAGMGWGMVPEVQAEPLLGDGRLVRLAPDDAPGRSVVVPLYWQQ
                     WKLDSPALAAVADAVAAEAAEALDGDRPD"
     misc_feature    8472..8490
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: medium, score: 26.73"
                     /tool="antismash"
     misc_feature    complement(8477..8495)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.1"
                     /tool="antismash"
     gene            8512..9582
                     /locus_tag="KV381_RS00635"
                     /old_locus_tag="KV381_00635"
     CDS             8512..9582
                     /GO_function="GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459230.1"
                     /locus_tag="KV381_RS00635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_00635"
                     /product="methyltransferase"
                     /protein_id="WP_218784236.1"
                     /transl_table=11
                     /translation="MTGSQSQETGNGRETAVDWQARGTVARLVLGQMATQALGTAVRLG
                     VFDRIGPGELSGDTLAGSLGTHPQATLRLLRALAGLQLLSETEPGVFRTTTAGDALRAD
                     TPGTMVAMARMFTDPVMLRGWDLLDESVRTGETTFDTVFGTDFFGHLKEQPELSAAFNE
                     AMSQGTRLTASNVPHHFDFGRFRTLVDIGGGDGTLLASVLRAHPELRGVLFDTAEGLAQ
                     APRRLAEQDVTGRVVLETGDFFASAPAGGDLYLLKSIIHDWDDERCAAILRRIRAVIPD
                     HGSLLIVEPVLPATVPADPAGGVYLSDLNMLVNVGGRERTEDDFAALCAAGGFALRSVT
                     PLPAPDIFRIIEASPV"
     gene            9665..10339
                     /locus_tag="KV381_RS00640"
                     /old_locus_tag="KV381_00640"
     CDS             9665..10339
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971207.1"
                     /locus_tag="KV381_RS00640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KV381_00640"
                     /product="response regulator transcription factor"
                     /protein_id="WP_030082193.1"
                     /transl_table=11
                     /translation="MRVLLVEDDDDLREVIASGLRDGGFAVDCAADWPEADVLLHVTAY
                     DCVVLDRMVPSGDTLVPLEVRRRAGWSVPVLCLTALDSLPERLRGLESGADDYLAKPFA
                     MPELVLRVRGLSRRAAARLPSFLSCADVVMDVARYEVRRGGVLLSLSPKEYAVLQQLLV
                     HRDAVVTRTDLLEHCWDEMADPVSNVVDAVVAGLRRKLGSPGLVHTVRGQGFLLSAEPG
                     PS"
ORIGIN
        1 acccgcacgg accggcggag gatcgtcgcc cgctcctgcc cggcgatcac ccgcgcgctc
       61 cctccgcggg cggtgccccg cgcctcgccc ggcggggcag gcggacggcg gcgggcgggg
      121 gccgttgcgg tgtcgcgtcc gccgggagcc gctgtgcgct catgccgcgc gctcctctcc
      181 ctgggccggc ccccggtgac gcccgtagga ctgcctctgg ccattgtcgg ccgggaccgg
      241 gccgcccgcg cgcgatggcg aagtgaccgc gcggagcgag atcgggccgt gcggcccggc
      301 gggcggtcgg gccctttgcg ctgggggcgg gaaggcgtcc gccccgagca cctggtgctg
      361 ggggcggacg cctgcgggga tcacacggcc gggcggtcgt ccgcatggtg ggggcgggcc
      421 gtggtggctc agatggtcgc cgtgtcgatc acgaagcggt agcggacgtc gctcgcgaga
      481 acccgctcgt acgcctcgtt gacctggctc gcgccgatca cctcgatctc tgcgccgagc
      541 ccgtgcacgg cgcagaagtc cagcatctcc tgggtctccg cgataccgcc gatcgccgaa
      601 cccgcgatcg accggttgcc catgatcagc gagaagaggt tcagcgagac cggctcctcg
      661 ggagcgccca cgttgacgag ggtgccgtcc gtgcgcagga ggccgaggta ggcgccgaag
      721 tccagcgggg ccgagaccgt ggagaggatg acgtcgaagg ttccggccag ttcttcgaag
      781 gtcttggggt cgctggtcgc gtagtagtgg tcggcgccca gcttgagccc gtcgtccttc
      841 ttccgcaggg actgggagag gaccgtgacc tcggcgccga gcgcgtgcgc gatcttgacg
      901 gccatgtggc cgagaccgcc gaggccgacg acggcgacct tcttgccggg gcccgcgccc
      961 cagcgcttca gcggtgagta cgtggtgatg ccggcgcaca ggagcggcgc ggcctcgtcg
     1021 agggcgatgc cctcggggat ggtgacggcg aacttctcgg tcacgacgat gtgcgtcgag
     1081 tagccgccgt aggtgggctc gccgctgcgg tcgagagcgt tgtacgtctg ggtgttgccc
     1141 tcggcgcagt gctgctcgcg gcccatcagg caggcgtcgc acgttccgca ggagtcgacc
     1201 atgcagccga cgccgacgcg gtcgccgacc ttgaaccggg tgaccccgga gccggtctcg
     1261 gccacgatcc cggcgatctc gtggccgggc accatcggga agatgccctc gccccagccg
     1321 tcgcgggcct ggtggatgtc ggagtggcag atgccggcga acttgatgtc gatcaggatg
     1381 tcgaactcgc ccaccggacg acgctcgatc gtggtgcgct ccagcggggc cttggcacgg
     1441 ggagcggcgt aggcggcaac ggtggtcatg ccggaggttc tcctctgggt gatgcggtag
     1501 gaacgtgtcc cagcctcact ccgggtccga cgtttaccca gcccaccgct ttgcctacgt
     1561 ccggtggtcc tactactgac ggggacaggc tcggccgcct acgaccactg atcgcaggaa
     1621 taatggaacc catggacgat cagccggagg ccgccgcgcc cgagccgctg gaccggcgtg
     1681 ccgagctcag tgagttcctg cgcacccgca gggccaagct tcagccgcag gacgtgggac
     1741 tgccggagtt cggccgggcc cgtcgggtgc ccgggctgcg ccgggaggag ctggcgcagc
     1801 tggccggggt gtccgtggcg tactacacgc gtctggagca gggcaacggg cgcaatgtgt
     1861 cggccgaggt gctcgacgcg atcgcccgcg cgctgcggct caccgacgcc gagcacgcgc
     1921 atctgaacca tctggcgagg ccggcccggc acaagaagaa gcgccgcccg gcgaaggtgc
     1981 agcgggtgcg gaccgggctg ctgtatctcc tggacaacat ggagggcatt cccgcgtacg
     2041 tggccggggc gcggtccgac atcctcgcct ggaatccgat ggcggcggcg gtgttcggcg
     2101 actgggccgc actgccgccg gccgagcgga actgggcgcg cctggtgttc ctctcccccg
     2161 cctaccggga cctgttcctg aactgggact ccaaggcctc ggacatggtc agctatctcc
     2221 ggctgtacgc gggttgccac cccgacgatc cgcagctgtc ggcactggtg ggcgaactct
     2281 cgctgaagag cgaggagttc cggcggctgt gggcgacgca caacgtcaag gagaagggcc
     2341 acggcatcaa gctgatccgg catccgctcg tcggcgacct gaccctgtcg tacgagacgc
     2401 tgaacctccc ggacgacgag gaccagcagc tggtgaccta ccacgcggag ccggggtcgg
     2461 agtcggccca ggccctgcgc ctgctggcga gctggggcgc cgacgcgggc cgtgcggatg
     2521 tgatcagcgg ggcggggcgg ggtccggacg acgagcgatg aggacgaact cccggcccgg
     2581 ccggtcgggt gcgtcacgca cctcgcgcac gacgaatccc tgggcggtca ggtccgcctc
     2641 ggcctcctga cgctcgcgga accgcagcgt ggagtcggag gtgaggacct ggccgtcggc
     2701 cgcgaagacg tacgtccaac ggaacgacac gagcggcccg gcgacctcgg tcacctcgac
     2761 ccagctctcg acggggccga ggccggcgat ctcggtgaca cggtgcgagc gctcccggtt
     2821 ccactcctcc caggcgcgcc gggccgggtt ccgcgtctcg acgaccagat gcccgcccgg
     2881 ccgcagcgcc tcgtaggcgc cgcgcagcgt cgcccgccag tcgcgcgggc cggtgatctc
     2941 ctgcgccacg ttggccgtca tggtgaccag gtcgacccgc aacggcggaa gagccgaagc
     3001 gtcaccgctg atccagcgga cccggtcacc gcccggtttg gcgcgcgcca cgtcgaccga
     3061 ggcccgggcg gggtcgacgc cgacgacctg gaacccgcgg tccgccagca gcagggcgaa
     3121 cactccggtg ccgcagccga tgtcagttca gtcggcttcg gccaccgccg ccgaagccac
     3181 ctccccggac gccactcctg ccgccgcctc cgcctcatcg gtcgcacccg ccgcgggcgc
     3241 gggaatccgg ggcgacgacg tcgtacccgt gccggccggg tccaggagcg gcagggtctc
     3301 cgcggcggcg agccggggca gcagcaactg ccagaagccg gtgagcgtgt acggggacag
     3361 ccacccggga tcgtgccggc cgagcacctc gaatccggcc gtcgcggcga ccgtggcggc
     3421 ggccgcgtcc tcgcgggaga cgtcgtcggc gagtgttccg gcggcggccg cctcgtccag
     3481 cagttcccgc acacggtggt gccactgcac tctcaggtcc ggggcggcgg ttcccgcggc
     3541 gtcgcagctc agtcggaatc cggcccggct caccgggtcc tcgcgcagcc gttccgcgag
     3601 agcgtgcgag ctgtcgacga gcgcctgcag ggcggtgtcc gtcctgcgcc ggatcgcgcc
     3661 ggacatctcg cgcagcaccc gggacgcctc caccaccacc gcatccgcta ccgcggcctt
     3721 gttctcgaag tggaagtgca gcgcgcccgt gctgacgccc gcgccggaac tgatcagcgt
     3781 cagccgcgcc tgcgcgtagc cgtgctgctc gaagacgacg gcggcggagc ggataagcga
     3841 ccggcgggta cgggtggctc gctcctgttt ggtcaccggg gcctccgttc gaagaatcgg
     3901 cggccgggca ggggggcgga aaaggcggct gccccggtgg agggctcccc gccggggcag
     3961 tgggacggct tcatggagtt atcaaaccaa cttaccggtt tcatttccag cgaacggtag
     4021 aacacggtgg aggcttcggg atccctttcc ggccacttct tcccttcagg gccgtcaaac
     4081 cggaccgaaa gggaaaagga aaagggcgga tcatcgcact tattggagat ctgtcacgcg
     4141 gcattacgcc gagaacgcgt ccgcgtgatc ggccgaccag atccggaacg gccgggccgg
     4201 tcgccctgtc aattcctcga cggtgggcac caccgccgcc ttcgcgcccg cccgctgccg
     4261 ctccgcgctc tccagaaagg cctccgcgac gggccgcggg tacctcgcca gcagggccgt
     4321 gcgcgcggcg tccacgccca gttcctcgaa gcgcaacggc cgctccagca cccgggagag
     4381 ctcggccgtc tgctcccgcg cggtgatcgc ctccgggccc gacacggcgt acgccctgcc
     4441 ctcgtgaccc gtaccggtga gtgcggtgac ggccaccgcc gcgacgtcgc gcgggtcgac
     4501 gcacgcgacc ggcgcgtcgc cgtacagcgc gcgcaccact ccggccgacc ggatgccggg
     4561 cgcccaggac agcgtgttgg acatgaacgt cctggggcgg acgaacgtcc aggcgacccc
     4621 cgactcccgc accgcctgtt cgttctcccg ctgacgccga gtgatgaagt cgtcggcccc
     4681 cggctcctcc accgccatca tggacagctt gaccaggtgg cggacgcccg ccgctgccgc
     4741 cgccgcggcg acccactcgt cgtggggctc cgtggggctc ttcgtcacca ggaacacgga
     4801 gctgacgccc cgcatggccc ggtcgagggc ggagcggtcc ccgtactcgc cctcgacgac
     4861 ctcgaccccg ctcccccgta ccgtcagccg ctcgggactg cgtgcgagaa tccgcaccgg
     4921 gcctgccgcc gccagctgtc cggcgacctc acggcccacc gctccggtcg cacccgtcac
     4981 gagaatcact cggaaacctc tcagccgttg gcccgcgggc cggacagaac ggtggagaac
     5041 accagttcgc cgtcctgatg gcctgtcacc tggaccgtca ccgtatcctc ggccgccccc
     5101 ggccggacca ccgccccgat cgagcacggg ctgtcgaact cggcgtaccg gtggaaccgg
     5161 ctgcgcgcct ccgccggaac gattccgccg ggtcccaccg ccaggcaggc cgcctgacgg
     5221 gccgcctcca gcagcagcat gcccgggaca tggtcgttgg gccgctggaa cagggtcggg
     5281 tgccgggtgt ccacgcgcag ttcccacaca ccgtcccggc cggcctccga gagcaccacg
     5341 tcctcggccc tggtgcggcc cgccccggcg gtggagaccg gcgtagcccg cggtatcgat
     5401 atcccctcgg cgggtgtgtc accgcgcatc cgccggtaca ccttggggtt cgtgaagcgg
     5461 gtcgcgcccg tccccgtagc ggccagttcc ccgtcgcggt ggaccgccca ccccacgcgc
     5521 ccctgtacgg gcagtccgcc ccgccacttc aggtcggagc agaacacctc cacgtcgatc
     5581 tcggcgggcg cgccgccgac gcccaggtgg tccaggtcgc acgcgtagtc gaggtccgcc
     5641 atcaggaagt ggtagcccac cgggacgccg tacccggcgt ggaaggagag catggccgcc
     5701 tgtcgcatcg cttccgccac cagcagcgga tcgtgccggt cgccgctcac cggggcgaag
     5761 aacggatgat cgtgcggcag gaccgccgcg accgagaacc gatcgcgtgc caggcgtacc
     5821 cagtgacgcg gaaacgcgtc ctccggcctg gaccggtgca ccatgtcgat ccccacggga
     5881 tgcaccacct cggcctccga atccactgcc ggcaccggat cgatcaagac tgctgcgtcg
     5941 gtcataactc cccccagggg tcatgtgatg gcgagccgtt cctgcgatgc acgtgcgctg
     6001 ataagataca gactaagcgg ttttttttct atccccggca gggccgtccc cccacaccgt
     6061 cccagccgtt tcccgggccc tcgcccagca aaggagacag gatggccaag caggaccggg
     6121 cgatccgcac ccgccagaca atcctgatgg cagcggcgca ggccttcgag aaatacggct
     6181 atcaagccgc gacgatcacc gagatactca aaagctccgg cgtcaccaaa ggcgccctct
     6241 acttccattt cccgtccaag gaagatctcg cgctcggtgt tctcgatgcg caggagccac
     6301 ctcagaccgt tccggaccag gctctcaaac tccaggaact catcgacctg ggcatgctgt
     6361 tcagccatcg tctgcagacc agcctcctcg cccgggcggg cgtccgcctc tccatggacc
     6421 agcaggcaca gggcctcgac cgcagcggcc cctttctccg ctggcaggaa gccacccatc
     6481 acctcctcac ccgtgccaaa cacaacggcg agctgctccc ccacgtcaac cccaccgaga
     6541 cggcggacct gtacgtggcc gccttcgcgg gaacccaggc ggtctcccag actctcacca
     6601 actaccagga cctgcagcgc cgccacatca cgctgcagca gcacatactc cccagtatcg
     6661 ccacgccctc catcctcacc gctctcgacc tgacaccgga gcgcgccgag cgcctcggcc
     6721 tgctggatcc ggtcggctga accgggccgg gggaacggcc gcccggcttt caggttcgta
     6781 cgcgggacgg gtgccgggcg ttcaggcgcc cagcgccagt ttcgcgccga ggccgatcat
     6841 catcacggcg accagcgcgt ccagcacccg ccaggcggca ggccgcgcca ggaccccgct
     6901 cagcagccgg gcgccgtatc cgagcgcgac gaaccagcac aggctggcga ggaccgcgcc
     6961 gaggccgaag gtccagcgca gcgggccccg gtcggcggcg acggaaccga gcaggaacac
     7021 ggtgtcgagg tacacgtgcg gattgagcca ggtcatcgcc aggcaggtca gcacgacgcc
     7081 ccggcgcgac gtggccggcg cgtcggcggc gtacagggcc gaggggcgca ggacgcggcg
     7141 tgcggcgagc aggccgtagc cgatcaggaa caggccgccc agcagcccca ccaccgtcag
     7201 cgccgacggc caggccacga cgaccgcacc gaccccggcg acccccaggg tgatgagcac
     7261 cgcgtcggac agggcgcaga tcccgaccac cgcgagcaca ccgtcacggc ggacgccctg
     7321 gcgcagcacc agggcgttct gggcgccgat ggcgacgatg agggagaggc cggtgccgaa
     7381 tccggcggcc gcggtggtca gggcgttcat catgcggccg acgctacaaa gcgggacaat
     7441 gtgatgtcca gctaaggatc cttacgtatc attagcgttc atgatgttcg agcttcctct
     7501 cgaccaggta cggacgctgc tggccgtggt cgacgaaggc accttcgacg cggcggcggg
     7561 cgtcctgcag ctgacgccgt cggcggtcag ccagcgcgtg aaggcactgg agcagcgcac
     7621 gggccgggtg ctgctgatgc gcacgaagcc ggtgcggccc accgagtccg gcgaggtggt
     7681 ggtgcgcttc gcgcgccagc tgtcccgact ggagcgcgat gcgcggacgg cgctcgggat
     7741 ggcgggtccg gacgggccga cgcccctgtc gatcgcggtg aactccgatt ccctggcgac
     7801 ctggttcctg cccgccctgc gacgggtgcc ggaggagctg gacctctgct acgagctgcg
     7861 ccgcgaggac caggaccata cggccgccct gctgcgggag gggctggtga tggcggcggt
     7921 gacctcgtcc ccggaagcgg tgacgggctg ttcggtgcgc ggactgggcc ggatgcggta
     7981 tctgccggtg gccagtcccg ctttcgccga ccggtggctg gagcggcggg ccggcacggc
     8041 gctgcacgag ctgatcggcg atgccccggt gatcggcttc gaccggcgcg acgacctcca
     8101 ggacgcgttc gtgcgcgcgc tcggcctcgg gccccgggcg agcgcccggc gtcatctggt
     8161 gcccacctcg gaggggttcg tgaacgccgt ggcggcgggc atgggctggg gcatggttcc
     8221 cgaggtccag gcggagccgt tgctgggcga cggccggctg gtgcggctcg ccccggacga
     8281 cgcccccggc cggagcgtcg tcgtcccgct gtactggcag cagtggaagc tggactcgcc
     8341 ggccctggcg gccgtggccg acgcggtggc ggccgaggcg gccgaggcgc tcgacggcga
     8401 ccggccggac tgaccggttc tgcctcaccc ccgcccaccc ccgcgtatgg gtgattgcgg
     8461 caccctccgg acgaggttag gtttacctaa ccaaccagag aggggcgtgc gatgacgggc
     8521 agtcagagcc aggaaaccgg caacggccgg gagacggcgg tcgattggca ggcgcggggt
     8581 acggtcgccc ggctcgtgct cggccagatg gccacccaag cactcggcac ggcggtgcgg
     8641 ttgggggtgt tcgaccggat cggtcctggc gagctgagcg gcgacacgct cgccggttct
     8701 ctcggcaccc atccgcaggc gacactgcgt ctgctgcgcg cgctcgccgg actccagttg
     8761 ctgtccgaga cggagccggg cgtcttccgg accacgacgg cgggcgacgc cctgcgcgcc
     8821 gacacgcccg gcacgatggt cgccatggcc cggatgttca ccgatccggt catgctccgg
     8881 ggctgggacc tgctcgacga gagcgtccgt acgggagaga ccacgttcga cacggtgttc
     8941 gggacggact tcttcggcca tctcaaggag cagcccgagc tgtccgccgc gttcaacgag
     9001 gcgatgagcc agggcaccag gctgaccgcc tcgaacgtgc cgcaccactt cgacttcggg
     9061 cgcttccgca cgctcgtgga catcggcggc ggcgacggca ccctgctggc ctcggtcctg
     9121 cgcgcgcacc cggagctacg gggcgtcctg ttcgacacgg ccgagggact cgcgcaggct
     9181 ccgcggcggc tggccgagca ggatgtgacc ggccgcgtcg tcctggagac cggtgacttc
     9241 ttcgcctcgg ccccggccgg gggcgacctc tatctgctga agagcatcat ccacgactgg
     9301 gacgacgagc ggtgcgcggc catcctgcgg cgcatccggg ccgtcatccc cgaccacggt
     9361 tcgctgctga tcgtcgagcc ggtgctgccc gcgacggtgc cggcggaccc ggcgggaggc
     9421 gtctacctca gcgatctcaa catgctcgtc aatgtcggcg gccgggagcg gaccgaggac
     9481 gacttcgccg ccctgtgcgc ggcgggcggc ttcgccctcc ggagcgtgac gccgttgccc
     9541 gcgcccgaca tcttccggat catcgaggcc tcaccggtct gacggccgcc tcggggcggg
     9601 cgcgcgggac gccttaggat ggcgggtctc tcccccttgg aacgacatcg gaggcgtcct
     9661 ccccatgcgt gtgctgctgg tggaggacga cgacgacctg cgggaggtca tcgcgtccgg
     9721 gctgcgggac gggggcttcg ccgtggactg cgcggcggac tggccggagg cggatgtgct
     9781 gctgcacgtc accgcgtacg actgtgtggt gctggaccgg atggtgccct ccggcgacac
     9841 cctcgtcccg ctggaggtca ggcgccgggc cggctggtcg gtgcccgtgc tgtgtctcac
     9901 cgccctggac tcgctgcccg agcggttgcg ggggctggag agcggcgcgg acgactatct
     9961 ggccaaaccg ttcgcgatgc cggagctggt gctgcgggtc cggggcctga gccggcgggc
    10021 ggcggcccgg ctgccgtcct tcctgagctg tgcggatgtg gtcatggacg tggcgcggta
    10081 cgaggtgcgg cgcggcggcg tgctgctgtc gctgagcccg aaggagtacg cggtcctcca
    10141 gcagctcctc gtccaccggg acgccgtcgt cacccgtacc gatctgctgg agcactgctg
    10201 ggacgagatg gccgacccgg tgtccaatgt ggtggacgcc gtcgtcgcgg ggctgcggcg
    10261 caagctcggc tcccccggcc tcgtgcacac cgtgcgcggt caggggttcc tgctgtcggc
    10321 ggagccgggg ccgtcctgat ggcgaagccc gtacggcgtc gttccccggc ccggaggcgg
    10381 ctgcgccggc tgcagctgcg gctgaccgcc gcgtacaccc tgatcaccgt cgtcggcctg
    10441 gccgcgctgt cctggctggt gatccgtacc gacgaccggg cgcggcagga cgcggagtac
    10501 gacgagatgc gccgccgggc gtccgtcgcg gcctcgctcg tctactacga ggacgaccgc
    10561 atccggctgg acggcctcga cgacgacgat gcgacctcgg gcaccccgca gatcctggtg
    10621 ctggagggac ggcccggcgg tgatccggtg accgtgttcc ggggccgcac cgaacagttc
    10681 gccgtaccgg ccggggctct tcgcgcggtg gcccgtacgg cgatgggcga ggaggagccg
    10741 gtgcgggccg aggcgcgcga ccgcacggga gagcccgtgc atctgctggc cgtgcccttc
    10801 taccacgacg ccaccgacga ggtggcgggc gcggccgtcg cggtcggcga tcctgagtac
    10861 ggcgcggccg agcaccgcag tctggtcctc tccctcgtcg tcgggaccgg ggcgctgacg
    10921 gcgctggccg cgctgaccgg gcatg
//
