LOCUS       NZ_JAHTMR010000012     14896 bp    DNA     linear   CON 21-AUG-2024
DEFINITION  Pseudomonas sp. PD9R Contig_12_consensus_sequence, whole genome
            shotgun sequence.
ACCESSION   NZ_JAHTMR010000012
VERSION     NZ_JAHTMR010000012.1
KEYWORDS    WGS; RefSeq.
SOURCE      Pseudomonas sp. PD9R
  ORGANISM  Pseudomonas sp. PD9R
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 246225)
  AUTHORS   Tournay,R., Firrincieli,A., Parikh,S. and Dominic,S.
  TITLE     Isolation and characterization of arsenic-tolerant endophytes and
            the potential of endophytic biofilms in arsenic tolerance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 246225)
  AUTHORS   Tournay,R., Firrincieli,A. and Doty,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2021) School of Environmental and Forest Sciences,
            University of Washington, 4000 15th Ave NE, Seattle, WA 98195, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-JAN-2020
            Assembly Method                   :: Geneious Prime v. 2020.0.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 158.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019200975.1-RS_2024_08_21
            Annotation Date                   :: 08/21/2024 05:49:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,297
            CDSs (total)                      :: 6,222
            Genes (coding)                    :: 6,093
            CDSs (with protein)               :: 6,093
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 61
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 2 of 129
            Pseudo Genes (frameshifted)       :: 24 of 129
            Pseudo Genes (incomplete)         :: 110 of 129
            Pseudo Genes (internal stop)      :: 10 of 129
            Pseudo Genes (multiple problems)  :: 15 of 129
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:28:33
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 187162
            Orig. end                         :: 202058
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHTMR010000012.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..14896
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="NAGGN"
                     /region_number="2"
                     /rules="((TIGR03104 or cds(GATase_7 and Asn_synthase)) and
                     TIGR03103 and (TIGR03106 or Peptidase_M42))"
                     /tool="antismash"
     cand_cluster    1..14896
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="((TIGR03104 or cds(GATase_7 and
                     Asn_synthase)) and TIGR03103 and (TIGR03106 or
                     Peptidase_M42))"
                     /kind="single"
                     /product="NAGGN"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..14896
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:9896](+)"
                     /cutoff="10000"
                     /detection_rule="((TIGR03104 or cds(GATase_7 and
                     Asn_synthase)) and TIGR03103 and (TIGR03106 or
                     Peptidase_M42))"
                     /neighbourhood="5000"
                     /product="NAGGN"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..9896
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="((TIGR03104 or cds(GATase_7 and
                     Asn_synthase)) and TIGR03103 and (TIGR03106 or
                     Peptidase_M42))"
                     /neighbourhood="5000"
                     /product="NAGGN"
                     /protocluster_number="1"
     gene            complement(1128..2642)
                     /gene="pap"
                     /locus_tag="KUF73_RS22215"
                     /old_locus_tag="KUF73_22220"
     CDS             complement(1128..2642)
                     /EC_number="2.7.4.33"
                     /GO_function="GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA]"
                     /GO_process="GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="pap"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650478.1"
                     /locus_tag="KUF73_RS22215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_22220"
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="WP_218440164.1"
                     /transl_table=11
                     /translation="MFESAEIDHAIDKETYDAEVPALREALLEAQFELQQQKRFPVIIL
                     INGIEGAGKGETVKLLNEWMDPRLIEVRTFDQQTDEELARPPAWRYWRMLPAKGRMGIF
                     FGNWYSQMLQGRVHGEFKDPRLDQAINAAERLEKMLCDEGALIFKFWFHLSKKQMKARL
                     KALADDPLHSWRISPLDWQQSETYDRFVKFGERVLRRTSRDYAPWHVIAGMDARYRSLV
                     VGKILLEGLQDALKRAKIHPEKVSAAPVFPSVDQLNLLDSLDLTLQLSKDDYEEQLITE
                     QARFSGLMRDKRMRRHALVAVFEGNDAAGKGGAIRRVAAALDPRQYNIVPIAAPTEEER
                     AQPYLWRFWRHIPARGKFTVFDRSWYGRVLVERIEGFCSAADWLRAYSEINDFEEQVTD
                     AGVIVVKFWLAIDKQTQLERFQAREEIPFKRFKITEDDWRNRDKWDDYRAAVGDMVDRT
                     STEISPWTLVEANDKRWARVKVLRTINHALEAAFEKSDKQARKGKK"
     gene            2839..4818
                     /gene="mnmC"
                     /locus_tag="KUF73_RS22220"
                     /old_locus_tag="KUF73_22225"
     CDS             2839..4818
                     /GO_function="GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /GO_process="GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="mnmC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008017762.1"
                     /locus_tag="KUF73_RS22220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_22225"
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase
                     MnmC"
                     /protein_id="WP_218440165.1"
                     /sec_met_domain="DAO (E-value: 4e-49, bitscore: 161.9,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPVLPHAQLDWDDQGRPYSRVFDDVYFSDKSGLDETRYVFIEQN
                     RLAERFAALPQGGRLVIGETGFGTGLNFLCAWQVFEQHAAAGARLHFVSVEKYPLSPAD
                     LQRALALWPELKAFSDQLLAQYVAIHQGFQRLTLANGRVTLTLLIGDALEQLPQLDAQV
                     DAWFLDGFAPAKNPDMWSAELFAELARLAAPGSTISTFTSTGWVRRLLNAAGFKMKRTP
                     GIGHKWEILRGEFLGWPPEVLACPPNKPWFARPASLTGERHALVIGAGLAGCASAASLA
                     ARGWQVTVLERHADVAQEASGNPQGVLYLKLSAHGTTLSQMIISGFGYTRRLLEHLQRG
                     TDWDDCGVLQLAFNAKETERQLQLAEAFPEDLLHLLDQPQAQALAGIGLAHGGLYYPEG
                     GWVHPPALCQWQAAHPQIQVLAHHDVLELRKTDGEWQAWDGERLLASAPVVVLAGAAEI
                     QRFPESAELPLKRIRGQITRLVQTPQSQSLSTVVCAEGYVAPARLGEHTLGASFDFKSE
                     DLTPTTAEHLGNLAMLEEISHDLVTRLQIEELAPESLQGRAAFRCTSPDYLPIVGPLAD
                     NQAFAEAYAALSKDARQVPDIECPWLDGLYVNSGHGSRGLITAPLSGELLAAWLDNEPL
                     PLPRSVAEACHPNRFALRRLIRGK"
     gene            5001..6773
                     /locus_tag="KUF73_RS22225"
                     /old_locus_tag="KUF73_22230"
     CDS             5001..6773
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     TIGR03104"
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     GATase_7"
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252149.1"
                     /locus_tag="KUF73_RS22225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_22230"
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="WP_218440216.1"
                     /sec_met_domain="TIGR03104 (E-value: 5e-295, bitscore:
                     973.0, seeds: 10, tool: rule-based-clusters)"
                     /sec_met_domain="GATase_7 (E-value: 2.3e-43, bitscore:
                     140.9, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 2e-83, bitscore:
                     274.8, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCGLAGELRFDNQPADLAAIERITHHLAPRGPDAWGFHSQGPIAL
                     GHRRLKIMDLSNGSAQPMIDHQLGLSLAFNGAIYNFPELRTELEDLGYAFYSGGDTEVL
                     LKGFHAWGEALLPKLNGMFAFAIWQRDTQSLFIARDRLGVKPLYLSRTGQRLRFASSLP
                     ALLKGGDINPILDPVALNHYLNFHAVVPAPRTLLAGIEKLPPATWMRIDADGTTQQKTW
                     WTLPYGPRADEMDLTLEDWRDRVLDSTRDAVAIRQRAAVDVGVLLSGGVDSSMLVGLLR
                     EVGVENLSTFSIGFQDAGGERGDEFQYSDLIAKHYGTHHHQLRIDEKEIIEQLPAAFRA
                     MSEPMVSHDCIAFYLLSREVAKHCKVVQSGQGADELFAGYHWYPQVDGASDPYAAYREA
                     FFDRSYDDYAATVQPKWLTENDAAGDFVKQHFAQPGADAAVDKALRLDSTVMLVDDPVK
                     RVDNMTMAWGLEARTPFLDYRLVELSARVPGKFKLPDGGKQVLKEAARLVIPSEVIDRK
                     KGYFPVPGLKHLQGDTLNWVRELLLDPSQDRGLFNPAMLDRLLTDPQGQLTPLRGSKLW
                     QLAALNLWLSEQGI"
     gene            6777..8525
                     /gene="ngg"
                     /locus_tag="KUF73_RS22230"
                     /old_locus_tag="KUF73_22235"
     CDS             6777..8525
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ngg"
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     TIGR03103"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008013604.1"
                     /locus_tag="KUF73_RS22230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_22235"
                     /product="N-acetylglutaminylglutamine synthetase"
                     /protein_id="WP_218440166.1"
                     /sec_met_domain="TIGR03103 (E-value: 6.8e-271, bitscore:
                     893.2, seeds: 7, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 2.9e-10, bitscore: 33.9,
                     seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPHATAINQRLLRGQTPSYERLQARLAEDGSELNGAPIAVHCGW
                     GRLLIGHTFADAASLAQELLNEQPGERDIALYVAAPQQVLGLEPTQLFLDPSDTLRLWF
                     SDYRQATRVFRGFRIRRAQSDADWSAINQLYQARGMLPIDATLLTPRHLGGPVYWLAED
                     EDSGAVIGSVMGLDHHKAFNDPEHGSSLWCLAVDPHCSRPGVGEVLVRHLIEHFMSRGL
                     SYLDLSVLHDNRQAKNLYAKLGFRNLSTFAIKRKNGINQPLFLGPGPEAAFNPYARIIV
                     EEAHRRGIDVQVDDADAGMFTLSHGGRRVRCRESLTDLTSAISMSLCQDKSLTHKVLKA
                     AGLNLPAQQLAGNADDNLAFLDEHQRVVVKPLDGEQGQGVAVDLQSIEDVQQAIESARQ
                     FDSRVLLESFHEGLDLRILVIGFEVVAAAIRRPAEVVGDGQHSIGALIEAQSRRRQAAT
                     DGESKIPLDQETQRTLHAAGYDYSSILPAGEHLFVRRTANLHTGGILEDVTAILHPTLM
                     DAAVRAARALDIPVVGLDLMVGAADQPAYVFIEANERAGLANHEPQPTAERFVDLLFPH
                     SQPAMP"
     gene            8706..9896
                     /locus_tag="KUF73_RS22235"
                     /old_locus_tag="KUF73_22240"
     CDS             8706..9896
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     TIGR03106"
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     Peptidase_M42"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899671.1"
                     /locus_tag="KUF73_RS22235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_22240"
                     /product="osmoprotectant NAGGN system M42 family peptidase"
                     /protein_id="WP_218440167.1"
                     /sec_met_domain="TIGR03106 (E-value: 1.6e-178, bitscore:
                     586.5, seeds: 7, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_M42 (E-value: 9.7e-75, bitscore:
                     245.1, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIKYPEPDLTYLQKVLLEMLAIPSPTGFTDTIVRYVAERLEELG
                     IPFEMTRRGTIRATLKGKKNSPDRAVSAHLDTIGAAVRAVKDNGRLTLAPVGCWSSRFA
                     EGSRVSLFTDNGVIRGSVLPLMASGHAFNTAVDEMPISWDHIELRLDAYCATRADCDSL
                     GISVGDFVAFDPLPEFTESGHISARHLDDKAGVAALLAALKAIVDNGEELLIDCHPLFT
                     ITEETGSGAAAALPWDVSEFVGIDIAPVAPGQHSSEHAVSIAMQDSGGPYDYHLSRHLL
                     RLASDNELPVRRDLFRYYFSDAHSAVTAGHDIRTALLAFGCDATHGYERTHIDSLAALS
                     RLLGAYILSPPVFASDAQPAKGSLDRFSHQIEHDTQMESDTRVPSVDSLVGQRNDS"
     gene            10080..10307
                     /locus_tag="KUF73_RS22240"
                     /old_locus_tag="KUF73_22245"
     CDS             10080..10307
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008017754.1"
                     /locus_tag="KUF73_RS22240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_22245"
                     /product="YheU family protein"
                     /protein_id="WP_205892131.1"
                     /transl_table=11
                     /translation="MLIPHDQLEVDTLTRLIEDFVTRDGTDNGDDTPLETRVLRVRQAL
                     SKGQALIVFDPESEQCQLMLKHDVPKHLFD"
     gene            complement(10296..10487)
                     /gene="csrA"
                     /locus_tag="KUF73_RS22245"
                     /old_locus_tag="KUF73_22250"
     CDS             complement(10296..10487)
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_process="GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /GO_process="GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="csrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060847.1"
                     /locus_tag="KUF73_RS22245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_22250"
                     /product="carbon storage regulator CsrA"
                     /protein_id="WP_218440168.1"
                     /transl_table=11
                     /translation="MLVLSRVVGELISIGDSISVRVVAINGGNVRFGVEAPQNVNVHRS
                     EIYERIQRKLARNKKSVE"
     gene            complement(10634..10966)
                     /locus_tag="KUF73_RS22250"
                     /old_locus_tag="KUF73_22255"
     CDS             complement(10634..10966)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007942060.1"
                     /locus_tag="KUF73_RS22250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_22255"
                     /product="YnfA family protein"
                     /protein_id="WP_218440169.1"
                     /transl_table=11
                     /translation="MLNYLWFFLAALFEIAGCFAFWMWLRQGKSAWWIVPALLSLTLFA
                     VLLTRVEAVYAGRAYAAYGGIYIIASIGWLAVVERIRPLGSDWLGVALCVIGASIILFG
                     PRFSAS"
     misc_feature    10964..10982
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.06"
                     /tool="antismash"
     misc_feature    complement(11028..11048)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 24.2"
                     /tool="antismash"
     gene            complement(11031..11846)
                     /locus_tag="KUF73_RS22255"
                     /old_locus_tag="KUF73_22260"
     CDS             complement(11031..11846)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (4-162). E-value: 1.6e-10. Score:
                     33.1. Matches aSDomain:
                     nrpspksdomains_KUF73_RS22255_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461756.1"
                     /locus_tag="KUF73_RS22255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_22260"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_218440170.1"
                     /sec_met_domain="adh_short (E-value: 4.6e-34, bitscore:
                     111.8, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQNRMMITGAGSGLGREIALRWAREGWQLALSDVSEPGLQESLKL
                     VREAGGDGFTQRCDVRDYSQLTAFAQACEVKLGGIDVIVNNAGVASGGFFSELSLEDWD
                     WQIAINLMGVVKGCKAFLPLLEKSKGKIINIASMAALMQGPAMSNYNVAKAGVVALSES
                     LLIELAQEEIGVHVVCPSFFQTNLLDSFRGPTPAMKAQVGKLLESSPISAADIADYIYQ
                     QIAAGEFMILPHEQGRMAWALKQKNPQLLYNEMTLMADKMRAKAKQTTG"
     aSDomain        complement(11361..11834)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KUF73_RS22255_PKS_KR.1"
                     /evalue="1.60E-10"
                     /label="KUF73_RS22255_PKS_KR.1"
                     /locus_tag="KUF73_RS22255"
                     /protein_end="162"
                     /protein_start="4"
                     /score="33.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="MMITGAGSGLGREIALRWAREGWQLALSDVSEPGLQESLKLVREA
                     GGDGFTQRCDVRDYSQLTAFAQACEVKLGGIDVIVNNAGVASGGFFSELSLEDWDWQIA
                     INLMGVVKGCKAFLPLLEKSKGKIINIASMAALMQGPAMSNYNVAKAGVVALSE"
     gene            12198..12350
                     /locus_tag="KUF73_RS22260"
                     /old_locus_tag="KUF73_22265"
     CDS             12198..12350
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299612.1"
                     /locus_tag="KUF73_RS22260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_22265"
                     /product="DUF3309 family protein"
                     /protein_id="WP_032833547.1"
                     /transl_table=11
                     /translation="MGTILIVILILLLIGGLPVFPHSRSWGYGPSGIIGVVLVVLLILL
                     LLGRI"
     gene            complement(12500..14593)
                     /locus_tag="KUF73_RS22265"
                     /old_locus_tag="KUF73_22270"
     CDS             complement(12500..14593)
                     /EC_number="2.7.8.-"
                     /GO_function="GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010208877.1"
                     /locus_tag="KUF73_RS22265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_22270"
                     /product="LTA synthase family protein"
                     /protein_id="WP_218440171.1"
                     /transl_table=11
                     /translation="MAIPDALNQQRASNRLLQPTVKSHLAYTLLCALVMMVMFSALRLA
                     LLVYNRSMILDTPASTFLEAFANGMRFDLRLVVYLSVPLLLALFSVRAMAARGLFRFWL
                     TVASSIALFLGLMEMDFYREFHQRLNGLVFQYVKEDPKTVMSMLWYGFPVVRYLLAWAV
                     GTWILSLAFKGTDRATRPRGPFSGGSIGTRQVAPWYARIVVFVVCLMICVVAARGTLRQ
                     GPPLRWGDVYTTDSNFANQLGLNGTLQLIAAAKSRMSDERSNIWKATIDQPVAQQTVRD
                     MLLTPSDKLVDAETAAVRRNYTPNADKTLPIKNVVVILMESFAGHSVGALGRPGNITPA
                     FDQLSKEGLLFDRFFSNGTHTHQGMFATMACFPNLPGFEYLMQTPEGSHKLSGLPQLLS
                     ARDYDDVYVYNGDFAWDNQSGFFSNQGMTNFIGRNDFVNPVFSDPTWGVSDQDMFNRGL
                     EELKARDGKDKKPFYALLQTLSNHTPYALPTPLPVERVTDRGSLNEHLTAMRYSDWALG
                     QFFEKARKEPYFKETLFVIVGDHGFGNEQQITEMDLGRFNVPMLMIAPGIQDKFGQRDH
                     TVGTQIDIVPTIMGRIGGDVVHQCWGRDLLNLPEGDKGFGVIKPSGSDQTVALLTADRV
                     LVLPKEMPPKLYQYELGANPKGELIPASADEAALKQKLESFLQTATKSLLDNTAGVVDG
                     KPD"
     misc_feature    complement(14772..14787)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.82"
                     /tool="antismash"
ORIGIN
        1 ccgttgacgt tgtacttgtc gttatcgatc tccagtcggt tacgcgcgta caggcattgc
       61 gaggcgaacg cttcgttgag ttcccacagg ccgatgtcgg caatctgtaa acccttggcc
      121 ttcagcagtc tcggcaccga gaacaccggg ccgatgccca tctcgtccgg ctcgcaaccg
      181 gccacggtaa aaccgcggaa aaacgctttg ggtttcaatc ccagctccag cgctttttcc
      241 aggctcatca ccagcgtcat ggaggcgcca tcagacagct gcgacgagtt gccagcggtg
      301 accgaaccgt cctcggcaaa cactggattc aacccggcca gactctgcag tgtggtatca
      361 ggccggttgc agtcgtcgcg atccacgacg ccgtcgacaa tctgcacctg accggtggcc
      421 ttgtcctcaa cccggtattt caccgccatc ggcacgattt catcatcgaa caacccggcg
      481 gcctgagcct gagcggtacg ctgctgactt tgcagggcgt acagatcctg ttcttcgcgg
      541 ctgacgctgt agcgacgagc gacgatttcg gcggtctggc ccatcggaaa gtagatgccc
      601 ggcacctgct ccttgagcag cgggttgatc aggttgtcgg tgttgacact tttcatggtc
      661 aggctgatgg actcgacgcc acccgcgaca atgatgtcgc tgcagccaga ggcaatctgg
      721 ttggcggcaa tcgcgatcgc ctgcaaaccc gaagaacaga aacggttcag agtcatgccg
      781 gcggtgccga tacccaagcg cgacagcacg gcgacgttgc gaccgatgtt gaacccctgg
      841 gcgccttcgt tggaaccggc cccgacaata cagtcctcga cgctggctgg atcgatgccg
      901 gtgcgtgtca gcaaggcgtt gacgcaatgc gccgccatgt cgtcaggacg ggtcatgttg
      961 aatttgccgc gaaaggattt ggccaggccg gtccgcacgc tgtcgacgat caccacttca
     1021 cgcatggcat acctcattgt tgttatcggt tgagagtgga ccgagcataa gcccacacaa
     1081 ttaccgaccg cgacaatcat tcaccccgcg tatgcgtaac catcgcttca ctttttgcct
     1141 ttgcgtgcct gcttgtcgga cttttcgaag gccgcttcca gcgcgtgatt gatcgtgcgc
     1201 aagaccttga cccgcgccca gcgtttgtca ttggcttcga ccagcgtcca cggcgagatc
     1261 tcggtgctgg tgcggtcaac catgtcgccg acggccgcgc gatagtcatc ccatttgtcg
     1321 cggttgcgcc agtcgtcctc ggtaatcttg aaacgtttga aggggatctc ctcgcgcgcc
     1381 tgaaaacgct ccagttgcgt ctgtttgtcg atggccagcc agaacttgac cacgatcacc
     1441 ccggcatcgg tgacctgctc ttcgaaatcg ttgatttcgc tgtaggcacg cagccagtcc
     1501 gccgcgctgc aaaacccctc gatgcgctcc accaacaccc ggccatacca ggaacggtcg
     1561 aacaccgtaa atttgccgcg tgccggaata tgtcgccaga aacgccagag atacggctgc
     1621 gcccgttctt cttccgtcgg cgcggcaatc ggcacgatgt tgtactggcg tgggtccagc
     1681 gctgcggcaa cccggcgaat cgccccgccc ttgccggccg cgtcgttgcc ttcgaacact
     1741 gccaccagcg catggcgacg catgcgtttg tcgcgcatca gcccggaaaa ccgcgcctgt
     1801 tcggtgatca gttgctcctc gtaatcgtcc ttggacagct gcaaggtcag gtcgaggcta
     1861 tctagcaggt tcaattgatc gacgctgggg aacaccgggg cggcgctgac tttctccggg
     1921 tgaatcttcg ctcgcttaag ggcgtcctgc aggccttcga gcaaaatctt gccgaccaca
     1981 aggctgcgat aacgcgcatc catcccggcg atcacatgcc agggcgcata gtcgcggctg
     2041 gtgcggcgca aaacgcgttc gccgaacttc acgaagcggt cgtaggtttc ggattgctgc
     2101 cagtccagcg ggctaatgcg ccagctgtgc aacggatcat cggccaaggc cttgagccgc
     2161 gctttcattt gtttcttgga aaggtggaac cagaacttga agatcagcgc gccttcatcg
     2221 cagagcattt tttccaggcg ttcagccgcg ttgatggctt gatcgagacg cgggtccttg
     2281 aactcgccat ggacccgccc ttgcagcatc tggctgtacc agttgccgaa gaaaatcccc
     2341 atgcgcccct tggccgggag catccgccaa taacgccagg ccggtggccg tgccagttct
     2401 tcgtcggttt gctgatcgaa agtacggacc tcgatcaggc gcgggtccat ccattcgttg
     2461 agtaacttga ccgtctcgcc cttgccggcg ccttcgatgc cattgatcag gatgatcacc
     2521 ggaaagcgtt tctgttgctg aagctcgaac tgcgcttcga gcaaggcttc gcgcagggcc
     2581 ggcacttcgg cgtcataggt ttctttgtcg atggcgtgat cgatttcggc agattcgaac
     2641 atggatggct cccttccaga tttcagtaag actagcggat cgggccgaac gtcagcgcag
     2701 aaagttcccc gtaggagcga gcgtgctcgc gatagacttc agggcaatgc gttgattcag
     2761 gaagcacgcg ttatcgttta cgtgcatcgc gagcaagctc gctcctacaa tggccgcctt
     2821 gccgttgccg agcctgccat gaaacctgta ttgccccacg cccagctcga ctgggacgac
     2881 caaggtcgcc cgtattcgcg cgtgttcgac gatgtgtatt tctccgacaa atccgggctg
     2941 gacgaaacgc gttacgtgtt catcgaacaa aaccggttgg cagaacgctt tgcagcgctg
     3001 ccacagggcg gtcggctggt catcggcgag accggtttcg gcacagggct gaattttttg
     3061 tgcgcctggc aggtgtttga gcagcacgca gcggcgggtg cgcggctgca ttttgtcagc
     3121 gtcgaaaaat acccgttgag cccggccgac ctgcagcgcg ccctggcgct gtggccggaa
     3181 ctcaaagcgt tttccgatca attgctggcg cagtacgtgg cgattcatca agggttccaa
     3241 cgcctgacat tggccaacgg acgagtaacg ctgacgttgt tgatcggcga cgccctcgaa
     3301 cagttgccgc aattggatgc gcaggtcgac gcatggtttc tcgacggttt tgcgccggca
     3361 aaaaaccccg acatgtggtc cgccgaactg tttgccgagt tggcgcgtct ggccgccccc
     3421 ggctcgacca tcagcacctt caccagtact ggctgggtaa gacgcctgct gaatgccgca
     3481 ggcttcaaga tgaaacgcac accgggcatc ggccacaaat gggaaatcct gcgcggtgag
     3541 tttcttggct ggccgcccga ggtgctcgcc tgtcctccca ataaaccctg gttcgcccga
     3601 cccgcatcgt tgaccggtga acgccatgcc ctggtgatcg gtgccgggct cgccggttgc
     3661 gccagcgcgg ccagcctggc agcgcggggc tggcaggtga cggtgctgga acggcacgcg
     3721 gatgtcgcac aagaagcttc gggcaatccg caaggcgtgc tttacctcaa gttatcggcc
     3781 cacggcacca cgttgtcgca aatgatcatc agcggtttcg gctacacccg acgcttgctc
     3841 gaacacttgc agcgaggcac cgactgggac gattgcggtg tgctgcaatt ggcgttcaac
     3901 gccaaggaaa ccgaacgtca actgcaattg gccgaagcgt ttccagagga tttgctgcac
     3961 ctgctcgatc agccccaggc gcaggccttg gccggcatcg gcctggcgca cggcggtctg
     4021 tattacccgg agggcggctg ggtccatccg ccggcgctgt gccaatggca agcagcgcat
     4081 ccgcagatcc aagtgcttgc gcaccatgat gtgctggaac tgcgtaaaac cgatggcgaa
     4141 tggcaagcct gggacggcga gcgtttgctc gccagtgcac ccgtggtggt gctggccggt
     4201 gccgccgaaa tccagcgttt ccctgaaagc gccgaactgc cgctcaagcg tattcgcggg
     4261 caaatcactc gactggtgca aaccccgcaa agccagtcgc tgagcacggt ggtctgtgcc
     4321 gaaggctacg tcgccccagc gcgtctgggc gaacacaccc tcggggccag tttcgacttc
     4381 aagagcgaag acctgacccc gaccacagcc gaacacctgg gcaatctggc catgctcgaa
     4441 gagatttccc atgatctggt cacgcgtttg caaatcgaag agcttgcccc tgaaagcctt
     4501 cagggacgtg ccgcctttcg ctgcaccagt cctgactact tgccgatcgt cggtcccttg
     4561 gccgacaacc aggccttcgc cgaagcctat gcggcgctca gcaaggacgc ccgccaagtg
     4621 ccggacatcg aatgcccgtg gctggacggc ctgtacgtca acagcggcca cggctcgcgc
     4681 ggcctgatca ccgcgccatt gtccggggaa ttgctggcgg cctggctgga caacgagccc
     4741 ctgcccctgc ccagaagcgt ggctgaagcc tgtcatccca accgttttgc cttgcgccgc
     4801 ttgatccgcg gcaaataatt acgcccccaa tcgcctggcc acccacatcg gccaggtgat
     4861 cgcttcgtcc ctcccgtcgc cgctgcttat aacccatcgt tctaaaactc ccactaccga
     4921 ggcgggtcag tttctgagta cccgccattt tggcacggta tcgaatgacc ctccccaacg
     4981 gaaaaaccgg taaggaattt atgtgcggat tagctggcga gttacgtttt gacaatcaac
     5041 ctgcagacct tgcagccatt gagcgaatca cccatcacct ggcccctcgt ggtcctgacg
     5101 cctggggttt tcacagtcag gggccgattg ccctgggcca ccgacgcctg aaaatcatgg
     5161 acctgtcgaa cggctcggcg cagccgatga tcgaccatca actgggcttg tccctggcct
     5221 tcaacggcgc gatatacaac ttcccggaac tgcgtaccga actggaagac ctggggtacg
     5281 ccttctattc cggtggtgac accgaagtgc tgctcaaagg tttccacgcc tggggcgaag
     5341 cgctgctgcc aaaactcaac ggcatgttcg ccttcgccat ttggcagcgc gacacacaaa
     5401 gcctgttcat cgcccgcgac cgtctcggcg tgaagccttt gtacctgtct cgcaccggtc
     5461 agcgcctgcg ctttgcttcg tcgctgccag cattgctcaa gggcggcgac atcaacccga
     5521 tcctcgaccc ggtggcactc aaccattacc tgaacttcca cgccgtggtc cccgcaccgc
     5581 gcaccttgtt ggcgggcatc gaaaaactgc caccagccac ctggatgcgc attgatgcgg
     5641 acggcaccac gcagcagaaa acctggtgga ccctgcccta cggcccacgc gccgatgaga
     5701 tggacctgac gctcgaagac tggcgcgacc gggtgctcga cagcacccgt gacgcggtgg
     5761 cgatccgtca gcgtgcggca gtcgacgtcg gtgtgctgct ttctggcggt gtcgattcga
     5821 gcatgctggt cgggttgttg cgcgaggtcg gcgtggaaaa cctgtcgacg ttttccatcg
     5881 gtttccagga cgccggcggc gaacgcggcg acgagttcca gtactcggac ctgatagcca
     5941 aacactacgg cacgcaccat caccaactgc gtatcgacga aaaagagatc atcgagcaac
     6001 tgcccgcagc gtttcgcgcg atgagcgagc caatggtcag ccatgactgc atcgccttct
     6061 acctgttgtc ccgggaagtg gccaagcatt gcaaagtggt gcaaagcggt cagggtgcgg
     6121 atgaactgtt cgccggttat cactggtatc cgcaggtgga cggtgcaagc gacccgtacg
     6181 cggcgtatcg cgaggcattt ttcgaccgca gctacgacga ctatgccgcc accgtgcaac
     6241 ccaaatggct gacggaaaat gacgccgcgg gtgatttcgt caaacagcat ttcgcacaac
     6301 cgggtgccga cgcggcggtg gacaaagcgt tgcgcctgga cagcacggtg atgctggtgg
     6361 atgacccggt caaacgcgtc gacaacatga ccatggcctg gggcctggaa gcgcgtacgc
     6421 cgtttctcga ctaccgcctg gtggagttgt cggcccgggt gccgggcaaa ttcaaactgc
     6481 cggacggcgg caagcaagtg ctcaaagaag ccgcacgact ggtgattccg agtgaggtga
     6541 tcgaccgcaa gaaaggctac ttcccggtgc cggggctcaa gcacttgcaa ggagacacct
     6601 tgaactgggt gcgcgaactg ctgctcgatc caagtcagga tcgcggcctg ttcaaccccg
     6661 ccatgctcga ccgactgctg acggacccgc aaggccagtt gaccccgttg cgcggctcca
     6721 agctgtggca attggcggcc ctgaacctgt ggctcagcga acaaggaatc tgaccgatga
     6781 aacctcacgc cacggccatc aaccagcgct tgttgcgcgg tcagacgccg tcctatgaac
     6841 gcttgcaggc gcgcctggcc gaagacggca gcgaattgaa tggcgcgccc atcgccgtgc
     6901 attgcggctg gggccggctt ttaatcggtc atacctttgc cgatgcggcc agccttgccc
     6961 aagagttact caacgaacag cccggtgagc gcgatatcgc cctgtatgtc gcggcccccc
     7021 agcaagtgct gggcttggag ccgacccagt tgtttcttga cccgtccgat accttgcgcc
     7081 tctggttcag cgattaccgt caggccactc gggtgtttcg tggctttcgc atccgtcgcg
     7141 cccagagtga cgccgactgg agcgcaatca atcagctgta tcaggcgcgc ggcatgctgc
     7201 caatcgatgc cacattgctg acgccacggc acttgggcgg cccggtgtat tggctcgccg
     7261 aagacgagga cagtggcgcg gtgatcggca gcgtcatggg ccttgatcat cacaaggcct
     7321 tcaatgatcc ggaacacggc agcagcctct ggtgcctcgc ggtggatccc cattgttcgc
     7381 ggccaggcgt cggtgaagta ctggtgcggc atctgattga acacttcatg agtcgtggcc
     7441 tgagctatct cgacctgtcg gtgctgcacg acaatcgcca ggcgaaaaac ctgtacgcca
     7501 agctcggttt tcgcaacctc tcgacgttcg cgatcaagcg caaaaacggg atcaatcagc
     7561 cgctgtttct cggtcctggc cccgaagcgg cgttcaatcc ctatgcgcga atcatcgtgg
     7621 aggaagcgca ccggcgcggc attgacgttc aggtcgatga cgccgacgcc ggtatgttta
     7681 ccctcagcca cggcgggcgc cgagtgcgtt gtcgtgagtc cctcacagac ttgaccagtg
     7741 ccatcagcat gagcctgtgc caggacaaaa gcctgaccca taaagtgctc aaggctgccg
     7801 gcctgaacct gcctgcgcag caactggcgg gtaatgccga cgataacctg gcgtttctcg
     7861 acgagcacca aagggtggtg gtcaaaccgc tggacggcga acaaggccag ggcgtcgcgg
     7921 tggacttgca gtcaatcgaa gacgtgcagc aagccatcga atcagcccgc cagttcgaca
     7981 gccgcgtgtt gctggagagc tttcacgaag gactggatct gcgcattctg gtcatcggtt
     8041 ttgaagtggt cgcggcagcg attcgtcggc cggccgaagt ggtcggcgat ggtcagcatt
     8101 ccatcggtgc gctgatcgaa gcccagagcc gacggcggca agccgccacg gacggtgaaa
     8161 gcaaaatccc gctggatcag gaaacccagc gcacgttgca tgcggccggt tacgactaca
     8221 gcagcattct gccggcgggc gagcatttgt tcgtacgccg tacggcgaat ttgcataccg
     8281 gcgggattct cgaggacgtc accgcgatcc tgcacccgac gttgatggat gcggctgtgc
     8341 gagccgcgcg ggcgctggat attcccgtgg tcggcctcga cctgatggtg ggcgccgccg
     8401 atcaaccggc gtacgtgttt atcgaagcca atgaacgtgc cgggctggcc aatcacgagc
     8461 cgcagccgac ggcggagcgc tttgtggatt tgttgtttcc gcatagtcag ccggcaatgc
     8521 cctagatgtg cagagcctga atcggcccct tcgcgggcgg gcctcgctcc tacaattccg
     8581 agtgctcgca atatcacgaa cagcacagcc cgtaggagcg aggcttgcac gcgaaggcgt
     8641 tggttcagac aacaaagatc cccctcatcg aaaccatcga tgcgctcaac ccatcaggag
     8701 tttccatgac tatcaaatac cccgaaccgg acctgacgta cctgcaaaaa gtcctgctgg
     8761 aaatgctcgc cattcccagc cccaccgggt tcaccgacac catcgtgcgc tacgtcgccg
     8821 agcggcttga agagttgggc atcccttttg aaatgactcg tcgcggcacc attcgcgcga
     8881 ccctcaaggg caaaaagaac agccccgacc gtgccgtttc cgcccacctc gacaccatcg
     8941 gcgcggcagt gcgcgcggtg aaggacaacg gtcgcctgac cctggcgccg gtgggttgct
     9001 ggtccagccg attcgccgag ggcagccgcg tgagtctgtt taccgataac ggcgtgatcc
     9061 gtggcagcgt attgccgttg atggcgtccg gccacgcgtt caatacggca gtggatgaaa
     9121 tgccgatcag ttgggaccac atcgaattac gtctggacgc ctattgcgcc acccgagccg
     9181 attgcgactc actggggatc agcgtcggcg atttcgttgc cttcgaccca ttgccggagt
     9241 tcaccgaaag cggtcacatc agcgcccgcc atctcgatga caaagccgga gtcgcggcgc
     9301 tgctggcagc gctcaaagcc atcgtcgaca acggcgaaga gttgctgatc gattgccatc
     9361 cgctgttcac catcaccgag gaaaccggca gcggcgcagc ggcggcgtta ccgtgggatg
     9421 tcagtgagtt cgttggcatc gacatcgcac ccgtcgcgcc cggccagcac tcaagcgagc
     9481 atgcagtgag tatcgcgatg caggattccg gcggccccta cgactatcac ttgtcacgcc
     9541 atctgctgcg cctggccagt gacaacgagt tgccggtgcg ccgcgacctg ttccgttact
     9601 acttcagcga tgcgcactcg gcggtcactg ccggccatga catccgcacc gccctgctcg
     9661 cctttggttg cgacgcgacc cacggctacg aacggaccca catcgacagc ctcgcggccc
     9721 tgagccgcct gctgggtgcc tatatcctca gcccaccggt attcgccagc gatgcgcaac
     9781 cggccaaggg ctcgctggac cggttcagtc atcagatcga acatgacacc cagatggaaa
     9841 gcgatacgcg ggtgccgtcg gtggatagcc tggtggggca gcgcaacgat agctagagag
     9901 gaacgcatgc aggagctgcc gcaggttgcg atcttttgat ctttggcagc cgttgaattg
     9961 ctcaaaatca aaagatcgca gcctgcggca gcgcctacag gtcatgcgtt catggcgcga
    10021 tgggtagctc tgactcatca atcgccgtag catcgcgcca ttgattaccc gaggtgccta
    10081 tgctgattcc ccacgaccaa ctagaagtcg acaccctgac ccgcctgatc gaagatttcg
    10141 tgacccgtga cggcactgac aatggtgacg acacaccgct ggaaacccgc gtattacgag
    10201 tgcgtcaggc attgagcaag gggcaggcat tgattgtctt cgacccggaa agcgagcaat
    10261 gccagttgat gctcaagcac gatgtaccca agcatctatt cgactgattt tttgtttcgc
    10321 gccagtttgc gctggatccg ctcgtagatc tcggaccggt gcacattgac gttctgcggt
    10381 gcttcgacgc cgaagcgcac gttgccgccg ttgatggcaa caacgcgcac ggaaatgctg
    10441 tcaccgatgg atatcaactc gcctacaaca cgactgagta caagcatggc cttcgtcctt
    10501 cagggttacc ggaccttgaa gatgcccggc acacgaaggg gcttcaagac aacggcagca
    10561 atccagaccg aacctacagc gaccgatccg ccttgctgac ggaaaggtct gacaaatcgt
    10621 ttaaacagat ccttcaggat gcggaaaaac gcggaccaaa caggatgatg ctcgccccga
    10681 tcacacataa cgccacacca agccagtccg atccgagcgg acgaatgcgt tcgaccaccg
    10741 ccagccagcc gatcgatgca atgatgtaaa tgccaccgta ggcggcatag gcgcgaccgg
    10801 cgtagaccgc ttcgacacgg gtcagcagca cggcgaacaa ggtcaggctg agcaacgccg
    10861 gcacaatcca ccaggcgctt ttgccctggc gcagccacat ccaaaaggcg aagcaaccgg
    10921 cgatttcaaa cagcgcggcg aggaaaaacc acaggtaatt gagcatgcga acgtctcgcg
    10981 agggtgacca gttgcggcca ccctaacgac gctgttgcgt gcgggcaagt tcagcctgtg
    11041 gtttgtttgg ccttggcgcg cattttgtcg gccatcaagg tcatttcgtt gtagagcagc
    11101 tgcgggttct tctgcttgag cgcccaggcc atgcggcctt gctcgtgggg caggatcatg
    11161 aactcgccag cagcgatctg ctgatagatg tagtcggcaa tatcggctgc gctgatgggc
    11221 gaactttcca gcaatttgcc gacctgtgcc ttcatggccg gggtcgggcc gcggaaagaa
    11281 tccagcagat tggtctggaa gaacgacggg cagaccacgt gtacgccgat ttcctcttgc
    11341 gccagttcga tcagcaggct ttcggacaac gccaccacgc cggcctttgc cacgttgtag
    11401 ttgctcatcg ccggaccttg catcagcgca gccatggagg cgatgttgat gatcttgcct
    11461 ttgctttttt ccagcaatgg caggaaggcc ttgcagcctt taaccacgcc catcaggttg
    11521 attgcgatct gccaatccca atcttccagg gacagttcac taaagaaacc gcccgatgcc
    11581 actccggcgt tgttgacgat gacatcaatg ccgccaagtt ttacttcaca ggcctgggcg
    11641 aaggcggtga gctggctgta atcacgcacg tcacaacgct gggtaaaacc gtcgccgccg
    11701 gcttcgcgca ccagcttcaa agattcctgc aggccgggtt cgctgacgtc cgacaaggcc
    11761 agctgccagc cttcacgcgc ccagcgcagc gcgatttcgc gacccaggcc agagcccgca
    11821 ccagtgatca tcatgcgatt ttgcatagca aacagccttg ttgttccggg gaagatgcgt
    11881 cgagtgtagc gaaggatatg gcccgaccca cgctccatca gattgctgaa tggcctgagc
    11941 aaagcgaaaa gatcgcaggc ttcccattga tcccggtggg agctgcagaa ggctgggatc
    12001 ttttggccaa tgcacgctct gcgcaactac cgccattcaa tcaaaggaaa taaccactgg
    12061 ttcgaaaaac atcgaaaaaa catggaattt tattgatagc gcaacagtcg gaatttttaa
    12121 gcagctcgaa ttaatcgcat tcccgctgac caataacgac taggtcattt ctaaacactt
    12181 acagcaagga aacagacatg ggcacaattc ttatcgtaat tctgatcctg ttgctgatcg
    12241 gtggtttgcc ggtcttcccg cactccagaa gttggggtta tggcccgtcc ggtatcatcg
    12301 gcgtggtatt ggtggtgctg ttgattctgt tgttgctcgg cagaatatga agatctgacc
    12361 agcaaaaaaa atgtaggagc gagcttgctc gcgatggact taaggacaac gcgtttattc
    12421 agccaatacg cgttatcgtt aacatccatc gcgagcaagc tcgctcctac aagtgtttgt
    12481 ttctttattg cgccgttatt taatccggct tgccgtcaac cacaccggca gtgttgtcga
    12541 gcaggctttt ggtagcggtc tgcaggaacg actcaagttt ctgcttcagc gctgcttcgt
    12601 cagccgacgc cggaatcagt tcccctttcg gatttgcacc cagttcgtac tggtagagtt
    12661 tcggcggcat ttcctttggc aacaccagca cgcgatcagc ggtgagcaag gctacggtct
    12721 gatcgctgcc cgatggcttg atcacgccaa aacccttgtc gccttccggc aggttcagca
    12781 ggtcacggcc ccagcattga tgcaccacat cgccaccgat acgacccatg atggtcggca
    12841 cgatatcgat ctgggtaccc acggtgtggt cacgctggcc gaatttgtcc tggatacccg
    12901 gagcaatcat cagcatcggc acgttgaatc gacccagatc catctcggtg atctgctgtt
    12961 cgttgccgaa gccatggtcg ccaacgatca cgaacagggt ttccttgaag tacggctctt
    13021 tgcgagcttt ctcgaagaac tgacccagcg cccagtcgga gtaacgcatg gcggtcaagt
    13081 gttcgttgag gctgccacgg tcggttacac gctcgaccgg caatggcgtc ggcagcgcgt
    13141 atggcgtgtg gttcgacagg gtttgcagca aggcgtagaa cggttttttg tccttgccgt
    13201 cgcgggcttt cagttcttcc aggccacggt tgaacatgtc ctggtcggaa acgccccacg
    13261 tcgggtcgga gaacaccggg ttaacgaagt cgttgcgccc gatgaagttg gtcatgcctt
    13321 ggttgctgaa gaaacccgac tggttgtccc aggcgaagtc gccgttgtag acatacacgt
    13381 cgtcgtaatc acgggcactg aggagctgtg gcaggccgga cagtttgtga ctgccttccg
    13441 gggtctgcat caggtattcg aaacccggca ggttcgggaa gcaggccatg gtggcgaaca
    13501 tgccctggtg ggtatgcgtg ccgttggaga agaaacggtc gaacagcagg ccttctttcg
    13561 acagttggtc aaaggctggc gtgatattgc ccggacgccc cagcgcgccc accgagtgac
    13621 cggcgaagct ttccatcagg atcacgacaa cgttcttgat cggcagggtc ttgtcggcgt
    13681 ttggtgtgta attacgacgt acggcggcgg tttcggcatc caccagttta tcgctcgggg
    13741 tcagcagcat gtcgcgcacg gtttgctggg caaccggctg gtcgatagtg gctttccaga
    13801 tgttgctgcg ctcatccgac atacggcttt tggctgccgc aatcagttgc aaggtgccat
    13861 tgaggcccaa ctgattggcg aagttcgagt cggtggtgta gacgtcaccc cagcgcaacg
    13921 gcgggccctg acgcaaggtg ccacgagccg cgaccacgca gatcatcagg cagaccacga
    13981 acaccacgat gcgcgcatac caaggggcta cctggcgagt gccaatgctg ccgccactga
    14041 acggaccacg aggacgggtc gcacggtcgg tgcccttgaa cgccaggctg agaatccagg
    14101 tgcctacggc ccaggccagc aagtaacgga ctactggaaa accgtaccag agcatgctca
    14161 tcacggtttt cgggtcttcc ttcacatact ggaagaccag gccgtttagg cgctggtgga
    14221 actcgcggta gaagtccatc tccatcaggc ccaggaacag cgcgatgctg gaagcaaccg
    14281 tcagccagaa acggaagagc ccacgcgccg ccatggctcg aacactgaac agcgccagca
    14341 gcaacggaac gctgaggtac accaccaggc gcagatcgaa acgcatgcca ttggcgaacg
    14401 cttcgaggaa agtcgaggcg ggggtgtcga ggatcatcga gcggttgtag accagcagcg
    14461 cgaggcgcag tgcggaaaac atcaccatca tgaccaaagc gcacagcagc gtgtaggcca
    14521 gatgcgattt gacggtcggt tgcagcaggc gattcgaagc tcgctgctga ttcagggcgt
    14581 ccgggattgc catgtcgttt taggacccat tggaagttga agtttcaaaa atacagctgc
    14641 gccctgccct ctattggcca gtacctggcc cggggggttg cgcggtgcgc aaatgttgca
    14701 cgatcaacgg ctgcattgcc attgattact gcccggcaaa cctgacgcgc gcgaattgtc
    14761 ttggagactt cgtgaaaatt tcgtgcaacg aatatccgga aacataataa agccaaaaag
    14821 taaaacgccc cgaaccagtc ggggcgctac tcaaagcact gtgcaattac ttctcagcgc
    14881 gctctttaag gctttt
//
