LOCUS       NZ_JAHTMR010000001     12202 bp    DNA     linear   CON 21-AUG-2024
DEFINITION  Pseudomonas sp. PD9R Contig_1_consensus_sequence, whole genome
            shotgun sequence.
ACCESSION   NZ_JAHTMR010000001
VERSION     NZ_JAHTMR010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Pseudomonas sp. PD9R
  ORGANISM  Pseudomonas sp. PD9R
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 986672)
  AUTHORS   Tournay,R., Firrincieli,A., Parikh,S. and Dominic,S.
  TITLE     Isolation and characterization of arsenic-tolerant endophytes and
            the potential of endophytic biofilms in arsenic tolerance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 986672)
  AUTHORS   Tournay,R., Firrincieli,A. and Doty,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2021) School of Environmental and Forest Sciences,
            University of Washington, 4000 15th Ave NE, Seattle, WA 98195, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-JAN-2020
            Assembly Method                   :: Geneious Prime v. 2020.0.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 158.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019200975.1-RS_2024_08_21
            Annotation Date                   :: 08/21/2024 05:49:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,297
            CDSs (total)                      :: 6,222
            Genes (coding)                    :: 6,093
            CDSs (with protein)               :: 6,093
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 61
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 2 of 129
            Pseudo Genes (frameshifted)       :: 24 of 129
            Pseudo Genes (incomplete)         :: 110 of 129
            Pseudo Genes (internal stop)      :: 10 of 129
            Pseudo Genes (multiple problems)  :: 15 of 129
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:28:33
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 805218
            Orig. end                         :: 817420
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHTMR010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12202
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12202
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12202
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7202](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7202
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    67..79
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 20.45"
                     /tool="antismash"
     misc_feature    complement(224..238)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.21"
                     /tool="antismash"
     gene            1782..3200
                     /locus_tag="KUF73_RS03700"
                     /old_locus_tag="KUF73_03700"
     CDS             1782..3200
                     /GO_component="GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /GO_function="GO:0015288 - porin activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1076: RND efflux
                     system, outer membrane lipoprotein,"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008018634.1"
                     /locus_tag="KUF73_RS03700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_03700"
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="WP_218436674.1"
                     /transl_table=11
                     /translation="MNALKFFLPSLLVSALAACTVGPDYKTPDTAPADIVYAKAANYDQ
                     SRFETVWWQQFDDPTLNQLVSKSLEGNRNLRVAFARWKAARAIRDDISNDNLPVVTSRV
                     SSQQGRSQVPGLTESRVNTERYDLGLDMAWEIDMFGRIQRELESSDAREDAAAADLFQL
                     RVTMIAELVDAYGQLRGAQLREKIALANLKNQNDSRSVTVSLRDAGVGNELDVMRADAR
                     LAAVEASVPQLQAEQVREKNRIATLLGERPEQLSVALNPADLPAIAKALPVGDPGELLH
                     RRPDILAAERRLASATADVGVATADLFPRVSLSGFLGFTAGRGSQIGSSAARAWSLGPS
                     ITWAAFDLGSVRARIRGANAEAEGALATYEQQVLLALEESENAFSDYGKRQQRLLSLIK
                     QSESSRAAASLAAIQYKEGSVDYLVLLDAERERLNAEDAQAQGETDQYRGIVAIYKALG
                     GGWDSGGNKVVAKN"
     gene            3353..4279
                     /locus_tag="KUF73_RS03705"
                     /old_locus_tag="KUF73_03705"
     CDS             3353..4279
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GFO_IDH_MocA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1079: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555299.1"
                     /locus_tag="KUF73_RS03705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_03705"
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="WP_218436675.1"
                     /sec_met_domain="GFO_IDH_MocA (E-value: 7.3e-21, bitscore:
                     70.9, seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQPIRLGLVGYGKIAQDQHVPAINANPAFELVSVATQGQPCAGVE
                     NFRSLSELLEKGPHVDAIAFCTPPQGRFALVQQALAAGKHVLVEKPPCATLGEAMALVD
                     QAAKQGVSGLFAWHSRYAPGIEAARDWLATRTLQSVQIDWKEDVRKWHPGQAWIWQPGG
                     LGVFDPGINALSIATHLLALPLFVESAELRVPSNCQSPIAASIKMSDARHLDVRAEFDF
                     DHGHDELWSIEIRCTEGTLRLDNGGALLSIDGVRQAVSKEGEYAAVYRHFQQLISAKTS
                     DLDLQPLRLVADSFFVGSRASVEPFYD"
     gene            4462..4845
                     /locus_tag="KUF73_RS03710"
                     /old_locus_tag="KUF73_03710"
     CDS             4462..4845
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003174080.1"
                     /locus_tag="KUF73_RS03710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_03710"
                     /product="VOC family protein"
                     /protein_id="WP_218436676.1"
                     /transl_table=11
                     /translation="MSAFTHVTVGTNDLDNARRFYDDVLGELGHKRIADLADNGSIWGE
                     DSPSFFVLKPADGAPASVGNGVTVSFEAPTRAAIDAFHAAALAAGGTCEGLPGPRGWAP
                     NAYAAYARDLDGNKLAAYCFKAD"
     gene            5001..7202
                     /locus_tag="KUF73_RS03715"
                     /old_locus_tag="KUF73_03715"
     CDS             5001..7202
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008030943.1"
                     /locus_tag="KUF73_RS03715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_03715"
                     /product="OsmC domain/YcaO domain-containing protein"
                     /protein_id="WP_218436677.1"
                     /sec_met_domain="YcaO (E-value: 1.3e-74, bitscore: 247.6,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIKVNFLDNLRLEAKFDDFTVVADQPIRYKGDGSAPGPFDYFLA
                     SSALCAAYFVKLYCDTRSIPTENIRLSQNNIVDPENRYNQIFKIQVELPADISDKDRQG
                     ILRSIDRCTVKKVVQAGPEFVIEEVENLDADAQALLMPASGSGAGTYIAGKDLPLEQTI
                     ANMSGIMAGLGMKIEIASWRNIVPNVWSLHIRDAHSPMCFTNGKGATKEGALASALGEF
                     IERLNCNFFYNDQFWGEEIANAAFVHYPNERWFQPGRKDELPAEILDEYCLRIYNRDGE
                     LRGSHLFDTNSGNEERGICSLPYVRQSDGEVVYFPSNLIENLYLSNGMSAGNTLAEAQV
                     QCLSEIFERAVKREIIEGEMALPDVPQDVLAKYPGILAGIQALEEQGFPVLVKDASLGG
                     EFPVMCVTLMNPRTGGVFASFGAHPSLEVALERSLTELLQGRSFEGLNDLPQPTFEGHA
                     VTEPNNFVEHFIDSSGVVSWRFFCAKSDYEFVEWDFSGQGENSNTEEAATLFGILENMG
                     KEAYMAVYEHLGAPACRILVPDYSEIYPVDDLIWDNTNKALFFREDILNLHSLDKAGLK
                     ALVKRLEESELDDYTDITSLIGIEFDDNTAWGQLTILELKLLIYLALQKFDEAKERVEM
                     FLQYNDNTVERGLFYQAVNVVLEMQLDEDLELEDYEVNFRRMFGNERMDAAIGSVDGSV
                     RFFGLTPTSMKLEGLDRHLRLIDSYKKLHTARANVTASS"
     gene            complement(7368..7991)
                     /gene="wrbA"
                     /locus_tag="KUF73_RS03720"
                     /old_locus_tag="KUF73_03720"
     CDS             complement(7368..7991)
                     /EC_number="1.6.5.2"
                     /GO_function="GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /gene="wrbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277342.1"
                     /locus_tag="KUF73_RS03720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_03720"
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="WP_218436678.1"
                     /transl_table=11
                     /translation="MPTKIQIVFYSSYGHIYKMAEAIAAGAREVGDVEVTLLQVPELMP
                     EEVLVKSGIKGYRAAFGSIPYATPEVLAEADAIIFGTPTRFGNMCSQMRNFLDQTGGLW
                     MSGGLIGKVGSVFTSTASQHGGQETTITSFHTTLLHHGMVIVGVPYSEQGLVNMTEISG
                     GSPYGASTLAGADGSRQPSENELQIARFQGKHVATIAKRLANNK"
     misc_feature    8215..8230
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.16"
                     /tool="antismash"
     gene            8351..9562
                     /locus_tag="KUF73_RS03725"
                     /old_locus_tag="KUF73_03725"
     CDS             8351..9562
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013646.5"
                     /locus_tag="KUF73_RS03725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_03725"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_218436679.1"
                     /transl_table=11
                     /translation="METLDGVVVVGGGPVGLLTALKLGKAGIRVVVLEAESGVSPSPRA
                     VAYMPPTAAALDRFGLLDDIRKRAVWCPDFAYRHGNGELIAKMDWSVLAQDTQYPYMLL
                     LGQNHVSNIIAQHLRELPNVEIRWNHRVEEVDQDDAYVTIQTSSPGGTSRLRARWLAAT
                     DGARSTVRQKVGLTFDGNTWAERLVATNVFYDFSLHGYSRANFVHDPVDWAVVVQLDKT
                     GLWRVCYGEDASLSDAEVRQRLPERFKRLLPGAPTPDQYRVDHLNPYRVHQRCAAEFRR
                     GRVILAGDAAHATNPMGGLGLSGGVLDAEHLAEALIAVIKNGASTKTLDEYSIDRRKVF
                     LEFTSPTATANFTWMKESDPGQRARDVAMFEHAGTDRSVMRQFLLDFEKLNGRRVIERK
                     LLAA"
     gene            9757..11394
                     /locus_tag="KUF73_RS03730"
                     /old_locus_tag="KUF73_03730"
     CDS             9757..11394
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422612.1"
                     /locus_tag="KUF73_RS03730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KUF73_03730"
                     /product="DUF1302 domain-containing protein"
                     /protein_id="WP_218436680.1"
                     /transl_table=11
                     /translation="MKAKTSFSLAPIRPSVHHLQLAMAAALLGLQGVPDVEAFALDTGN
                     PDYAIAFDNTVKYSAAWRLNNQDDAVRPTYNPNLDDGDNNFNRGLISDRLDLLSELDMS
                     YQRRYGVRVSGAAWYDDVYNRGNDNHTDTVNSLSVRNDQFTTDTRDFQGRDGELLDAFG
                     YGKFDLGAMSLSLRGGRYSQVYGESLFFGGNGIAVAQSPVDINKLLSVPSSTFKEIIRP
                     IGQIGGTLLVNQNVTLGAYYQYRWEQARLPAAGSYFSYADFLDEGGESFILGPGVFLRR
                     GKDIDARNSGQFGMQVKIKSGEFEYGLYAARYHDKFPQFYLRPGEGDYALVYGEDIRTF
                     GASVSTLIGETNVAAETSIRRNTPLAATGNVVVDFTGTGDGDHNPLYPVGNTFHAQVSA
                     ISVLANSGLWDGATLLGEVAYNRITSVDKNRDQLDPNVTRNASSLRMLFTPEYFQVFPG
                     VDMTVPLGIGYGISGNTAVLGTGFSPEGGGDMSLGLQFDYRKTWRGGITVTHYYGSAGG
                     VVDSNGALSGDQVYADRDFVAISLQRTF"
ORIGIN
        1 gggcagttct ataagcagtt cgccctgacc atcgccatct cgacggtgat ctcggccttc
       61 aactccctga ccctgtcacc tgccttggcg gcggtgttgc tcaaggccca tggcgtgccg
      121 aaggaccgtt tctccaaagt gctggaccgc ttgctcggcg gttggctatt caaacccttc
      181 aaccggctgt tcgaaaaggc cagccatggc tatgtggttg ccgtggccaa aatcatccgt
      241 ggcagcagcg tggcgttact gatttacgcc ggtttgatcg cgatgaccta catgggcttc
      301 agcaccacgc cgaccggttt tgtgccgacc caggacaaga agtacctggt gaccttcgcg
      361 caactgccgg acgcggccag cctggatcgc accgaatcgg tgattcgccg catgagcacc
      421 attgcgatga atgaaccggg ctttgacagc gccgtggcgt ttcccggtct gtcgatcaac
      481 ggcttcacca acagccccaa cgccggcatc gcgtttatcg gcttgcgtcc ctttgatgag
      541 cgcacagatc cgagcctgtc cgccaaggca atttccgctt cgttgaatgc caagttcggt
      601 ggcattcagg acgcctacat cgccgtgttc ccgccaccgc cggtgcaagg tttgggcacc
      661 attggcggct tccgcctgca gattgaagac cgcagcaacc tggggtatga cgagctgtac
      721 aaagagacga tgaacatcat caccaagagt cgtagtgttc cggaactggc caatctgttc
      781 accagctaca ccgtcaacgt gccgcaggtc gaagccgcca ttgaccggga aaaggccaag
      841 acccacggcg tagcgatcaa cgacatcttc gacaccttgc aggtttatct gggctcgctc
      901 tacgccaacg acttcaaccg ttttggccgg acctatcagg tcaatgtgca ggccgagcaa
      961 cagttccgcc aggagccgga gcagatcggt cagttgaagg tgcgcaatga ccacggcgag
     1021 atgatcccgc tggccacttt cgtcaaggtc agcccgacct ccggcccgga ccgggtctcg
     1081 cactacaacg gcttcatcac cgcagaaatc aacggcagcg cggcccccgg ctacagctcg
     1141 ggccaggccg aggcagcaat tgcaaaactg ctgaaagaag aactgcccaa cggcatgacc
     1201 tacgaatgga ccgacctgac ctaccaacag atcctgtcgg gtaacacggc gctgctggtg
     1261 ttcccgctct gcgtgctgct ggccttcctg gtattagcgg cgttgtacga gagctggagc
     1321 ctgccgctgg cggtgatcct gatcgtaccg atgaccctgc tgtcggccat tgccggggtg
     1381 atcatttcca agggcgacaa caacatcttc acccagatcg gtctgatcgt gctggtgggc
     1441 ctggcctgta aaaacgcgat ccttattgtc gagttcgcca aggacaaaca ggaccagggc
     1501 ctgtcaccgc tggacgccgt actggacgcg tgccgcatgc gtctgcggcc gatcctgatg
     1561 acctcattcg ctttcatcat gggcgtgatt ccactggtga cgtccaccgg cgccggttcg
     1621 gaaatgcgtc gcgccatggg cgttgcggtg ttttccggga tgctcggcgt gacctttttc
     1681 ggcctgctgt tgacgccggt gttcttcgtt ctgattcgcc gctacatcga gcgcaagcat
     1741 gcgcgtaagc aagccaacgc tctgaacctg gaggtgcaac cgtgaatgct ctgaagttct
     1801 ttttacccag tcttcttgtc agcgcgctgg ccgcatgcac ggtcggaccg gattacaaaa
     1861 cccccgatac ggcgccggct gacatcgtct atgcgaaggc ggccaactac gatcagtcgc
     1921 gctttgaaac ggtgtggtgg caacaattcg acgatcctac gctcaaccag ttggtgagca
     1981 aatccctgga aggcaaccgc aatctgcgcg tcgcgtttgc tcgctggaag gcagcccggg
     2041 cgattcgcga tgacatcagc aatgacaatc tgccggtggt caccagtcgt gtcagcagcc
     2101 agcaaggtcg ttctcaggtt ccggggctga ccgagagtcg ggtcaacacc gagcgctacg
     2161 atctggggct ggacatggcc tgggaaatcg acatgttcgg gcgtattcag cgtgagctcg
     2221 aatcgagcga cgcccgggaa gacgcggcag cggcggatct ctttcagttg cgcgtgacca
     2281 tgatcgccga actggtggac gcctatggcc agttgcgcgg tgcgcagttg cgggaaaaaa
     2341 tcgccctggc caacctgaag aatcaaaacg attcgcgcag cgtgacggtt agtttgcgtg
     2401 atgccggtgt tggtaatgaa ctcgacgtca tgcgggctga cgcgcgcctg gccgctgtcg
     2461 aggcgagcgt gccgcaactg caagctgagc aggtccggga gaaaaaccgc atcgcgacct
     2521 tgctgggtga acgtcccgag caattgagcg tggcgttgaa cccggcggac ctgccggcta
     2581 tcgccaaggc gttgccggtc ggtgatccgg gcgagctgtt gcatcgtcgg cccgacattc
     2641 tcgctgccga acgccgcttg gcatcggcca cggcagatgt tggcgtcgcc accgcagacc
     2701 tgtttcctcg ggtcagcctc agcgggttcc ttggctttac cgccgggcgc ggttcgcaga
     2761 tcggttccag cgctgccagg gcctggagtc tgggcccgag catcacctgg gccgcttttg
     2821 atttgggcag cgtgcgcgca cgcatccgtg gcgcgaacgc cgaagccgaa ggcgccttgg
     2881 ccacctatga acagcaggtg ttgctggcgc tggaagaatc ggaaaacgcc ttcagtgact
     2941 acggcaaacg ccaacagcga ctgctgtcgt tgatcaagca aagtgaatcc agccgtgcgg
     3001 cggcaagtct tgcagctatc cagtacaagg aagggtcggt cgattatctg gtgttgctcg
     3061 atgcggagcg tgagcgcttg aatgccgagg atgctcaggc ccagggtgaa actgatcaat
     3121 accggggcat cgtcgcgatc tataaagcgc tgggaggcgg gtgggacagc ggcggcaaca
     3181 aggttgtggc aaagaactga cgccagggtt aaatctggtg cccggtgtgg aaagggtcgt
     3241 tggtatttcg gtcggtcagc cgaggtttga agcaacccga tgacttccac gcgatacaaa
     3301 cgtaatcttg ccgactcgct tcccaagaca agaaaaaaaa caggagtcat tgatgcaacc
     3361 gatccgtctc ggtctggtgg gctacggcaa gattgcccag gatcaacacg ttccagctat
     3421 caacgccaac cccgcgtttg agttggtgtc tgtcgcaacg caagggcagc cctgcgccgg
     3481 ggtagagaac tttcggtccc tgagcgaatt gctcgaaaag ggccctcacg tcgatgcgat
     3541 tgcgttttgt acaccgccgc aaggacggtt tgcgctggtg caacaagcgc tggccgctgg
     3601 caaacacgtg ctggtcgaaa agccgccgtg cgccaccttg ggtgaagcaa tggcgttggt
     3661 ggatcaggcc gctaagcaag gcgtcagcgg tttgttcgcc tggcattcac gttacgcgcc
     3721 aggtatcgaa gccgcccggg actggctcgc tacccgcacc ctgcaaagcg tacagattga
     3781 ctggaaggaa gacgtgcgca agtggcaccc aggccaggca tggatctggc agccgggtgg
     3841 cctgggcgtc ttcgatcccg gcatcaatgc cttgtcgatt gcaacccatc tgctggcgct
     3901 gccgctgttt gtcgagtctg ccgaactgcg agtccccagc aactgccagt cgccgattgc
     3961 cgcttcgatc aaaatgtctg acgcgcgcca cctcgatgtt cgcgcggaat tcgatttcga
     4021 ccacggtcat gatgaactct ggagcatcga aattcgctgc accgaaggca ccctgcgcct
     4081 ggataacggt ggcgcgctgt tgagcattga tggcgtacgc caagcggtat cgaaggaggg
     4141 cgagtacgcg gcggtgtacc gacattttca acagctcatt agcgccaaaa ccagtgacct
     4201 ggacctccag ccgctacggt tggttgccga cagctttttc gtcggcagcc gggcgtcggt
     4261 tgagccgttc tacgattaac agccgctcga taaacggcag cgatcgctgc tgttttctat
     4321 gcgtgcgtca catgggatta atgcagatac atcactgttc tttcgcaggg acggggcaca
     4381 ttcttggccg aaacaccgac gcagcattgc gcgtcattct cggcctgatg gttcccgttt
     4441 ctaccaaaga gagcgataaa aatgagtgca tttacccacg taacagtcgg caccaacgac
     4501 ttggataacg cccgccgttt ctacgacgac gtactgggcg aacttggcca taaacgtatc
     4561 gccgatctcg cggacaacgg ctctatatgg ggcgaagact caccgtcctt ctttgttctg
     4621 aaaccggctg acggtgcacc tgccagtgtc ggcaatggcg taacggtcag tttcgaagcg
     4681 ccgacacgtg ccgcaatcga cgcttttcat gccgccgcat tggccgctgg cggtacgtgt
     4741 gaaggccttc cagggccaag aggctgggca ccgaacgctt acgcggccta tgcccgggat
     4801 ctggacggta acaaactggc ggcctattgc ttcaaagcgg attaaattgc gctcgcgcgg
     4861 cccttggtgg caaggcatcg agggcagcgc atgcgcctca aagcgcaaaa atctacagcc
     4921 tggcgcgtgg tacaagactt ccggctgaaa agccggataa tagcggccaa ttattcggct
     4981 cgttattctg gtactcccgc atggaaatca aggtcaattt tctcgacaac cttcgacttg
     5041 aagccaagtt cgatgacttc acggtggtgg ccgatcagcc tattcgctat aagggcgatg
     5101 gctctgcacc gggtccgttc gattactttc tggcttcgtc ggccttgtgc gcggcttact
     5161 ttgtaaagtt gtactgtgat acccgcagta tccctaccga aaatatccgg ctctctcaga
     5221 acaacattgt tgacccggaa aaccgctaca accagatttt caagattcag gtcgagttgc
     5281 cggcggatat ctccgacaag gatcgccagg gcattctgcg ttccatcgac cgctgcacgg
     5341 tgaagaaggt ggtgcaggcc ggccctgagt ttgtgatcga ggaagtggag aacctggatg
     5401 ccgatgcgca ggcgttattg atgccggcct cggggtcagg agcgggcacc tatattgcgg
     5461 gcaaggatct gccactggag caaaccatcg ccaacatgtc cggcatcatg gccgggctgg
     5521 gcatgaagat tgaaattgcg tcgtggcgca atatcgtgcc caacgtgtgg tcgctgcata
     5581 ttcgcgatgc gcactcgccg atgtgtttta ccaacggtaa aggcgcgacc aaagaaggcg
     5641 cgttggcgtc ggcattgggc gagtttatcg agcgactgaa ctgcaatttc ttctacaacg
     5701 atcagttctg gggcgaagaa atcgccaacg cggcgtttgt gcattacccc aacgagcgct
     5761 ggttccagcc tgggcgtaaa gacgaacttc cagccgagat tctcgatgag tactgcctgc
     5821 ggatttacaa ccgcgatggc gagttgcgtg gctcgcatct gttcgatacc aattccggca
     5881 atgaagagcg cggcatctgt tcgctgccct atgtgcgcca gtcggacggc gaagtggtgt
     5941 acttcccgtc caacctgatc gaaaacctgt acctcagcaa cggcatgagt gccggcaata
     6001 cgctggccga agcgcaggtg caatgcctgt cggagatctt cgaacgtgcg gtaaaacgcg
     6061 aaatcatcga aggcgaaatg gccctgccag atgtacctca agacgtgctg gctaagtatc
     6121 ccggcatcct ggccggtatc caggcgctgg aagagcaggg cttcccggtg ctggtcaagg
     6181 atgcgtcgct gggcggcgag ttcccggtga tgtgcgtgac cttgatgaac ccgcgtaccg
     6241 gcggtgtgtt cgcctcgttc ggcgcgcacc caagccttga ggtggcgctg gagcgcagcc
     6301 tgacggaatt gctgcagggc cgcagttttg aaggcttgaa cgacttgccg caaccgacct
     6361 tcgaaggcca cgcggtgacg gagccgaaca acttcgttga gcacttcatc gattccagcg
     6421 gcgtggtgtc gtggcgcttt ttctgcgcca aatccgacta cgagtttgtc gagtgggatt
     6481 tctccggcca gggtgaaaac tccaacaccg aggaagctgc gaccctgttc ggcattctcg
     6541 aaaatatggg caaagaagcg tacatggctg tgtacgagca tttaggtgca ccggcctgcc
     6601 gcattttggt gccggattat tcggaaattt atccggtaga cgatttgatc tgggacaaca
     6661 ccaacaaagc gctgtttttc cgtgaagaca ttctgaacct gcacagcctg gacaaggccg
     6721 gcctcaaggc gctggtcaaa cgtctggaag aaagcgaact ggacgactac accgacatca
     6781 ccagcctgat cggcatcgag ttcgacgaca acaccgcgtg gggtcagttg acgattctgg
     6841 agttgaagct gttgatctac ctcgccttgc agaagtttga tgaggcgaag gaacgggtag
     6901 aaatgttctt gcagtacaac gacaacacgg tggaacgcgg gttgttttac caggccgtga
     6961 atgtggtgct ggaaatgcag ttggacgagg acctggagct ggaagactac gaagtcaatt
     7021 tccgccggat gttcggcaac gagcggatgg acgcggcgat tgggtcggtt gacggcagcg
     7081 tgcgcttctt cggcctgacg cctacgagca tgaaactgga agggctcgat cgacacctgc
     7141 ggctgatcga cagctacaaa aagttgcaca cggcgcgggc caatgtgacg gcatccagct
     7201 aatagctctg ccgctttccc gagcagaccc gctcccacat ggggtcggca tgctccgtaa
     7261 ttgcgaagca ctgacagaaa aaataccccc ctgaaagatc gctcttcgag gggggtattt
     7321 ttttcaacgc atgcaaccgc gtgggggcga aagatgcttt gtgggtttta cttattgttg
     7381 gccagacgct tggcgatagt cgccacatgc ttgccctgga acctggcgat ttgcagttca
     7441 ttttcgcttg gctggcgcga gccatcggcg ccggccagcg tcgaggcgcc gtacggtgaa
     7501 ccgcccgaga tttcggtcat gttgaccagg ccttgctcgg agtagggaac gccgacgatg
     7561 accatgccgt ggtgaaggag ggtggtgtgg aaactggtga tggtggtttc ctggccgcca
     7621 tgttgcgagg cggtgctggt gaacacgctg ccgactttgc cgatcaaccc acctgacatc
     7681 cacaggccgc cggtttgatc gaggaagttg cgcatctgcg aacacatgtt gccaaagcgt
     7741 gtcggcgtac caaagatgat cgcatcggct tcagccagaa cttccggggt tgcgtacgga
     7801 atactgccga aggcggcgcg gtaacccttg atgccgcttt tgaccaggac ttcctcaggc
     7861 atgagttccg gaacttgcaa tagggtgact tctacatcac cgacttcgcg cgcgccggca
     7921 gctatggctt cggccatctt gtagatgtgg ccataagagc tgtagaaaac gatctgaatt
     7981 tttgttggca tgtcggtttc ctgatttgat ttctttgcgg cttatataac tgtccgagtt
     8041 aagcctgttt caaacgctta cacatatcgg ttggatgaat gaataacaac cgtcaatagc
     8101 gtttgcttgt tgcagcatga gcagcggcgc aaaacaacac aacgggatta aggttccgga
     8161 tttgcaaacg acctgattgg taaaaccaaa cgttaaaaat tgcgatcgtc aagttcgcca
     8221 ttggcaaaca gctttgcaat tgcctggcta agtcggggtg gttacgctgg atttagtcgc
     8281 ctggccaatc ttggttcaac ttccgagtgg cgggtcttcc ctgcaagcgg ttttcgatag
     8341 gaaaaataaa atggaaacgc tagatggagt ggttgtagtt ggcggaggtc cggtgggact
     8401 tctgaccgca ttgaaactcg gcaaggccgg tattcgagtc gtggtgcttg aagctgaatc
     8461 aggcgtttcc ccatcgccgc gcgccgtcgc gtatatgccc ccgaccgccg ctgctctgga
     8521 tcgttttggt ttgctcgacg acattcgcaa gcgcgcggtg tggtgcccgg acttcgccta
     8581 tcggcatggc aacggcgagc tgatcgcaaa gatggactgg agcgtcctgg cccaggatac
     8641 gcaatacccg tacatgctgt tgctagggca aaaccacgtg tccaacatca ttgcccagca
     8701 cctgcgggag ttgcccaatg tcgagattcg ctggaatcat cgggtcgagg aagttgacca
     8761 ggatgacgcc tacgtgacca tccagaccag cagccccggc ggtacgtcgc gcctgcgagc
     8821 ccgttggctc gcggccaccg atggcgcacg gagcacggtt cgccagaagg tcggtctgac
     8881 gttcgacggc aatacctggg ctgagcgact ggtcgccacc aacgtcttct atgacttctc
     8941 gctgcatggc tattcacgcg ccaacttcgt tcacgacccg gtcgattggg cggtggttgt
     9001 gcaactcgac aaaaccggcc tgtggcgtgt ttgttatggc gaagacgcgt cgttgtcgga
     9061 tgccgaggtc cgtcagcgcc tgcccgaacg attcaaacgg ttgctgccgg gcgcaccgac
     9121 accagaccag taccgtgtcg atcacctcaa cccgtaccgc gttcaccagc gctgtgccgc
     9181 cgagtttcgc cgtgggcggg taattctggc cggcgatgcc gcccacgcca ccaacccgat
     9241 gggtggcctc ggactgtccg gtggtgtgct ggatgccgag catttggccg aagcattgat
     9301 cgccgtgatc aagaatggtg cgtcgaccaa gaccctggat gaatactcga tcgaccgccg
     9361 caaagtattc ctggagttca cctcgccaac cgcaacggcc aacttcacct ggatgaagga
     9421 aagtgatcca ggccagcgtg ctcgtgacgt tgccatgttt gaacatgcgg gtactgatcg
     9481 ctcggtcatg cgccagtttc tacttgattt cgaaaagctc aatggtcgaa gagtaataga
     9541 aagaaaactg ctcgccgctt gaactaaccg aatgcaagaa gcctgagagc tgaaacgctc
     9601 ttgggctgca tcctcgtgtt tataacctgt catacgcaac tctttaaaag gacttaatga
     9661 tcgttcgaac aaaagacgta gtcctgcgcg cgactttcaa ctaaatgaaa gcgcgtcatg
     9721 tgtcccgaaa taataaaaat aaatagggtt catcatatga aagcgaaaac gagctttagc
     9781 cttgcaccga taaggccgtc ggtgcaccac ttgcagttag ccatggcagc tgcattgctg
     9841 ggattacaag gtgtacccga cgtggaggcg tttgcgcttg atacgggtaa ccccgattac
     9901 gcgattgcct ttgacaatac cgttaaatac agcgcggcct ggcggctcaa caatcaagat
     9961 gacgccgttc gcccgaccta taaccccaac cttgatgacg gcgacaataa cttcaaccgg
    10021 gggcttattt ccgatcggct ggacctgctg tcggaactgg acatgagtta tcagcgtcga
    10081 tacggggttc gggtcagtgg tgcggcctgg tatgacgatg tctataaccg gggcaacgac
    10141 aaccacaccg ataccgttaa cagcttgtcc gtgcgcaatg atcaattcac caccgacacg
    10201 cgcgactttc agggccgtga tggcgaactg ctggacgcct tcggctacgg caaattcgac
    10261 ctgggagcca tgtcgctgtc gctgcggggc gggcgctata gccaggttta cggtgagagc
    10321 ctgttcttcg gtggcaatgg catcgcggtg gcgcaatccc ccgttgatat caacaaattg
    10381 ctctcggtgc cgagttccac gttcaaggaa atcattcgcc cgattggcca gataggcggc
    10441 acgttgctgg tcaatcagaa cgtgaccctg ggtgcgtatt atcagtaccg atgggaacag
    10501 gcccggctgc cagcggcggg tagctatttc agctacgccg actttctcga cgagggcggt
    10561 gagtcgttca ttctggggcc tggcgtgttc ttgaggcgag gcaaggacat cgatgcgcgc
    10621 aactccgggc agttcggtat gcaggtcaag atcaagtccg gcgagttcga atacggcctg
    10681 tacgccgcgc gatatcacga caagtttcca cagttctacc tgaggcccgg ggagggtgat
    10741 tacgcgttgg tctatggcga agacatcagg accttcggcg ccagtgtcag cacgttgatc
    10801 ggcgaaacca acgtcgctgc cgaaacctcg attcggcgca ataccccgct ggctgccacc
    10861 ggcaacgttg tggtcgattt caccggaaca ggcgacggtg accacaatcc gctctacccg
    10921 gtgggcaata ctttccatgc ccaggtgtcg gccatctcgg tgctggccaa cagtggtttg
    10981 tgggacggcg cgaccctgct cggcgaggtg gcttacaacc gaatcaccag cgtcgacaaa
    11041 aaccgcgatc aactggaccc caacgtcacc cgcaacgcca gctccctgcg catgttgttc
    11101 accccggagt atttccaggt gttccctggc gtggacatga ccgtgccgct cggcatcggc
    11161 tatggcatca gcggcaacac cgccgtgctg ggcaccggct tctcgcccga gggcggcgga
    11221 gacatgagcc tgggcctgca attcgactac cgcaaaacct ggcgcggcgg catcaccgtc
    11281 acccactact acggcagtgc cggtggcgtc gtcgattcga acggcgcgct ctcgggcgat
    11341 caggtctatg ccgaccgcga tttcgttgcc atttccctcc agcgcacgtt ctagcccgaa
    11401 aggaatacaa caatgataaa caagacactc cttggcttca ctttcctcac cagcctgatc
    11461 ggtgcgctac cggtgtcggc tgccgtcagc ccgcaggaag cggccaccct gggcacaacg
    11521 ctcacccctt tgggcggtga caaagccgcc aatgccgatg ccagcatccc ggcctggaac
    11581 ggtggcacca aacaagtggc cgccactggc agcagcagta ttcctggcga tctgttcagc
    11641 gcggacaaac cgctgctgaa aatcactgcg gccaacatgg ccaagtactc cgacaagctg
    11701 tcggaaggca cccgcacact gctgcagaaa taccctgaca gtttccgtat ggacgtgtac
    11761 ccgacgcggc gcacggcgtc cgccccggac tacgtctaca agaacactgc gctcaacgcg
    11821 gtgaattgca aactcacgca aaacggccaa tcggtggagg gctgttttgg tggcattccg
    11881 ttcccggttc ccaagagcgg gcaggaagtg atctggaatt tcctgttgcg ggtcgagccg
    11941 gaatcggtgg aattcggctt caagaacgtg gtgctgacca gtgacggtgg tcagaccctg
    12001 gcgacccgaa acgacaacac gtttcaatac ccgtactact accagaaagg ttcggtactg
    12061 acctggtcca aggagtattt cctgcagcgt ttcagcacca cggcgccacc cttcaaggcc
    12121 ggggaaacac tggtgatacg cgacagcgtc gacccgcagc agtcccggca ggcctggcaa
    12181 tatctggttg gacagcggcg gg
//
