LOCUS       NZ_JAHHYI010000003     16904 bp    DNA     linear   CON 30-AUG-2024
DEFINITION  Priestia megaterium strain 179-F 3A1 HS
            NODE_3_length_969567_cov_82.799572, whole genome shotgun sequence.
ACCESSION   NZ_JAHHYI010000003
VERSION     NZ_JAHHYI010000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 969567)
  AUTHORS   Wood,J.M., Hendrickson,R., Singh,N.K., Bateh,J., Peres,J.B.,
            Aronson,H.S., Mason,C.E. and Venkateswaran,K.
  TITLE     Draft Genome Sequences of Sporulating Bacteria Isolated from a
            Spacecraft Assembly Facility
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 969567)
  AUTHORS   Wood,J.M., Hendrickson,R., Singh,N.K., Bateh,J., Peres,J.B.,
            Aronson,H.S., Mason,C.E. and Venkateswaran,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2021) Biotechnology and Planetary Protection
            Group, Jet Propulsion Laboratory, California Institute of
            Technology, 4800 Oak Grove Dr., Pasadena, CA 91109, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-SEP-2020
            Assembly Method                   :: SPAdes v. 3.11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 198.49x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019037625.1-RS_2024_08_30
            Annotation Date                   :: 08/30/2024 04:30:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,420
            CDSs (total)                      :: 6,353
            Genes (coding)                    :: 6,249
            CDSs (with protein)               :: 6,249
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 32 of 104
            Pseudo Genes (incomplete)         :: 71 of 104
            Pseudo Genes (internal stop)      :: 23 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:31:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 952663
            Orig. end                         :: 969567
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHHYI010000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..16904
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="3"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..16904
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..16904
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[10000:10849](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10849)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    305..318
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.99"
                     /tool="antismash"
     gene            complement(343..2022)
                     /locus_tag="KM925_RS15910"
                     /old_locus_tag="KM925_15905"
     CDS             complement(343..2022)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016763016.1"
                     /locus_tag="KM925_RS15910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15905"
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="WP_053486820.1"
                     /transl_table=11
                     /translation="MYIQLSQLSMEEQVECLTKALVKIKSINGTSGEVKIADFIKNTLQ
                     SFPYFKENPLHVWEQKINGDTLGRKNVFAFIQGSKKNAQTMIYHAHLDTVGIEDFGPLK
                     DIALDPEELQRYFSMHNINEDVQRDARSGEWMFGRGAVDMQSGIAVHLANVLHFTKHPN
                     KLEGNVLFMVNPDEESQHAGIISAVSELNRLKKEKNLSYVAAINTDFITPLYDGDSTRY
                     IYTGAAGKLLPCFYIYGREVHVGDTLAGIDPNLIASEITGSIHNNINLAENIEGELVLP
                     PSCLYQRDNKEAYNVQTAVSSHLYFNYFIYERTAKEVMSQLIGLSIEACEKVEKKLSDY
                     YELFVRRTNLPKRNLSWKVDVVSLEDYLEELDQKGINAQACIERAFEQYAHLELRTRCF
                     KVIEELQKLDPHQSPRVIVFFAPPYLPHNHLKKDESRDQQLLYPLQAAVEKVGKQTGET
                     FQFKKFFPYLADGSFLSLHETDEEIDAFTRNFPGWGIAGAIPFREIRELNIPSINIGVY
                     GKDGHKWTERVYKPYSFGVLPKLIQETTVQMLKSRHACTNHI"
     gene            complement(2126..3331)
                     /locus_tag="KM925_RS15915"
                     /old_locus_tag="KM925_15910"
     CDS             complement(2126..3331)
                     /GO_function="GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-350). E-value: 2.9e-120.
                     Score: 393.3. Matches aSDomain:
                     nrpspksdomains_KM925_RS15915_Aminotran_3.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461624.1"
                     /locus_tag="KM925_RS15915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15910"
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="WP_098239219.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.4e-137, bitscore:
                     454.5, seeds: 13, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 3.6e-06, bitscore:
                     22.2, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTETQKTVKIIEQTEKFGAHNYHPLPIVIEKAEGVWVHDPENNK
                     YMDMLSAYSAVNQGHRHPKIIEALKRQADKVTLTSRAFHNDQLGPWYEKICKLTNKNMA
                     LPMNTGAEAVETAIKAARRWAYDVKGVAENQAQIIACVGNFHGRTMTAVSLSSEAEYQR
                     GFGPMLPGLQTIPYGDLEALKAAITPNTAAFLIEPIQGEAGIVLPPEGFLKAAADLCKE
                     HNVLFIADEIQVGLARTGKMFACDWEGVEPDMLILGKALGGGVFPISCVVANEDILSVF
                     NPGSHGSTFGGNPLACAVSLAALEVIEDEELPARSLELGSYFKQKLQSLSNPVIKEVRG
                     RGLFIGVELHEEARPYCEKLKDQGLLCKETHSTVIRFAPPLTISKEELDWAIERIEKIF
                     AE"
     aSDomain        complement(2282..3247)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KM925_RS15915_Aminotran_3.1"
                     /evalue="2.90E-120"
                     /label="KM925_RS15915_Aminotran_3.1"
                     /locus_tag="KM925_RS15915"
                     /protein_end="350"
                     /protein_start="28"
                     /score="393.3"
                     /tool="antismash"
                     /translation="VIEKAEGVWVHDPENNKYMDMLSAYSAVNQGHRHPKIIEALKRQA
                     DKVTLTSRAFHNDQLGPWYEKICKLTNKNMALPMNTGAEAVETAIKAARRWAYDVKGVA
                     ENQAQIIACVGNFHGRTMTAVSLSSEAEYQRGFGPMLPGLQTIPYGDLEALKAAITPNT
                     AAFLIEPIQGEAGIVLPPEGFLKAAADLCKEHNVLFIADEIQVGLARTGKMFACDWEGV
                     EPDMLILGKALGGGVFPISCVVANEDILSVFNPGSHGSTFGGNPLACAVSLAALEVIED
                     EELPARSLELGSYFKQKLQSLSNPVIKEVRGRGLFIGVELH"
     gene            complement(3355..4902)
                     /gene="pruA"
                     /locus_tag="KM925_RS15920"
                     /old_locus_tag="KM925_15915"
     CDS             complement(3355..4902)
                     /EC_number="1.2.1.88"
                     /GO_function="GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA]"
                     /GO_process="GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="pruA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391658.1"
                     /locus_tag="KM925_RS15920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15915"
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="WP_013055370.1"
                     /sec_met_domain="Aldedh (E-value: 5.1e-156, bitscore:
                     515.6, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVSYSHEPFTNFKEENNKKAFQEALTYVNTQLGKTYPLVIDGEK
                     VETEEKITSVNPANIKEVIGYVSTVNKDLAEQAMQSALKAFETWKKWKVEHRADILFRA
                     AAIIRRRKHEFSSYLVKEAGKPWNEADADTAEAIDFLEYYARQVLTLKDGSPVQSRDGE
                     YNQYNYIPLGVGIIISPFNFPLAIMAGTAVAAIVTGNTILLKPADATPVVAAKFVEVME
                     EAGLPKGVLNFIPGATPEIGDYLVEHPKTRFVSFTGSRAVGCRIYERAAKVQPGQKWLK
                     RVIAEMGGKDTVVVDKDADLDLAASSIVYSAFGFAGQKCSAGSRAVVHQDVYDEVLEKA
                     VALTKTLTVGTPEEVSTYMGPVIHEASYNKVLSYIEIGKEEGRLVAGGEADNSTGYFIQ
                     PTIFADVDEKARLMQEEIFGPVVAFSKARDFDHMMEIANNTEYALTGALLSNNREHIER
                     AREEFHVGNLYFNRGCTGAIVGYQPFGGFNMSGTDSKAGGPDYLVLHMQAKTTSETF"
     misc_feature    complement(4949..4962)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     18.25"
                     /tool="antismash"
     gene            complement(5076..5207)
                     /locus_tag="KM925_RS32240"
     CDS             complement(5076..5207)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KM925_RS32240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_255256005.1"
                     /transl_table=11
                     /translation="MMENQTCSDFFVGRVNVGSCFLAVDWVDLGMSQYILYAKDIGI"
     gene            complement(5281..6315)
                     /locus_tag="KM925_RS15925"
                     /old_locus_tag="KM925_15920"
     CDS             complement(5281..6315)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1078: LacI family
                     transcription regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055369.1"
                     /locus_tag="KM925_RS15925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15920"
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_013055369.1"
                     /transl_table=11
                     /translation="MGVTIKDVANLANVAPSTVSRVIADSSRISEKTKQRVRSAMKELG
                     YHPNIIARSLANQTTEAVGLVMPSSADKVFQNPFFPEVLRGISTGAHENKRAIYMSTGE
                     TEEEIFAEVVQMVQGRRVDGLILLYSKVDDQVLSYLQEQSVPFVVIGKPFKHSESITFV
                     DNDNFKAGKEATEYLIESGHERIAFIGGSLELVVTIDRLTGYDKAIQKANIPYRDEYVI
                     HEEFLQKGGKEAVCELLSLKERPTALVVADDLMALGVLNTLDEMGISVPEDISIVSFNN
                     VLIAEMARPALTSIDINIFELGYQAVRCLIKQIETPYAVAEQVRVPHKMIKRQSCKTIQ
                     MTKR"
     gene            complement(6373..7887)
                     /locus_tag="KM925_RS15930"
                     /old_locus_tag="KM925_15925"
     CDS             complement(6373..7887)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Alpha-amylase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1257: Alpha-glucosidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055368.1"
                     /locus_tag="KM925_RS15930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15925"
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="WP_063670542.1"
                     /sec_met_domain="Alpha-amylase (E-value: 2.9e-27, bitscore:
                     91.9, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKWKRTSMLLILLLLFGSSASAQDHKDIRDEVIYSLMINRFYNGS
                     EQNDRNVNYERSDAYYGGDLQGIAQKLDYIQEMGFTAVLLTPFMENDETGYHGYAVTNH
                     YKIDDHFGTLKDLQNLVKKAHERKIKIMVEFPLTISNNHTWVADKGKQTWISNESGTDN
                     EVIKALPHLNLKESAVQKYLIKNAAWWSKQADIDGYYVKDIDQAPSAFISSFSKTLKSM
                     DPSFLLIGEINGQPLKKNEQADSLGVDLLVDRTLAEATAATFSGTNRPQKMLYDKWGKR
                     SNQPLANFLDDVHSTRFTAQALKSENHPGARTKQGLSYLYTSPSVPIVFYGTEIALNGG
                     KGAENYGMMNFLANPDIIDHLKKLAELRVKSPSLRKGSFTLVSEQKGVAVYKRRYKDET
                     TFVVINNTKETSVINVPLKKVGKENELRGLITEGIIRPVDDVYNISSEPETTNVYKVVK
                     KSGFNIGYIAAVAAVYTGFGIFLYKASSKRRKEKKISQSRKKDSAS"
     gene            complement(8037..9296)
                     /locus_tag="KM925_RS15935"
                     /old_locus_tag="KM925_15930"
     CDS             complement(8037..9296)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008519 - ammonium channel activity
                     [Evidence IEA]"
                     /GO_process="GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055367.1"
                     /locus_tag="KM925_RS15935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15930"
                     /product="ammonium transporter"
                     /protein_id="WP_371388974.1"
                     /transl_table=11
                     /translation="MHAGFAMVETGFTRSKNSLNILMKNMLTVAVSSVLYFVVGFALMF
                     GSTKGGFIGSSGFFLNGESDQMSFFVFQIVFAATCATIISGAVAERMKLSSYMMLTIAM
                     VAVIYPVVGHWVWGGGWLSKLGFVDFAGSTVVHLTGALGAAVAVIFLGARLGKYGSDGK
                     VNAIQGHNILLGALGVFILWFGWFGFNGGSTLAADPALVPGIIATTLMSGSCGVLSSAL
                     YTKFRYKRIDASLTLNGALAGLVGITAGTANVSIPGAIVIGLIAGIILVEAVHFIDSKM
                     KLDDPVGAITVHGICGIWGTLAVGLFDTANGLFYGGGVQLLGIQALGIIAVMGWTIGTV
                     GLFLFVLTRFSSIRVSSEEEIAGLDFAEHGSSAYEFLESFVATGDGNLHPEFGVGLIDR
                     LNKVGKVSDKPHISNVNETV"
     gene            complement(9474..9815)
                     /locus_tag="KM925_RS15940"
                     /old_locus_tag="KM925_15935"
     CDS             complement(9474..9815)
                     /GO_function="GO:0030234 - enzyme regulator activity
                     [Evidence IEA]"
                     /GO_process="GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055366.1"
                     /locus_tag="KM925_RS15940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15935"
                     /product="P-II family nitrogen regulator"
                     /protein_id="WP_013055366.1"
                     /transl_table=11
                     /translation="MKKVEAVIRPEKFQALRVKLEEVGINGLTVSEVAGCGQQKGEQGL
                     FRGQSFEIKLVPKVKVEMILETEQVDEVIKIIQSICSTNQIGDGKIFVYPVEDAVRIRT
                     GEAGVEAIL"
     misc_feature    complement(9878..9892)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 26.38"
                     /tool="antismash"
     misc_feature    complement(9941..9952)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.89"
                     /tool="antismash"
     gene            complement(10001..10849)
                     /locus_tag="KM925_RS15945"
                     /old_locus_tag="KM925_15940"
     CDS             complement(10001..10849)
                     /EC_number="2.5.1.-"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081717.1"
                     /locus_tag="KM925_RS15945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15940"
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="WP_053486816.1"
                     /sec_met_domain="phytoene_synt (E-value: 4.5e-73, bitscore:
                     241.6, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PT_phytoene_like (E-value: 1.2e-91,
                     bitscore: 303.0, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMNISLDKAYNQCEEIIKENSKTFYKAFSMLPSKQRKAVWAVYAF
                     CRQVDDIVDEGCNPEIELQQFESEFARFKNGELLNESAMWVALRNVFEQYEMNVQAFDD
                     MITGQRMDLVKKEYDTLDEVKTYSYHVASTVGLMLLPILAPTTHKELEEEAVALGIAMQ
                     LTNILRDVGEDLERGRIYLPKDLMNSYGYMRMLLENRWVTSEFIEMWEHLAKEAEMYYI
                     KGLQSIHKYPVASRLPLTGAAHLYRAILDKIRKENYDVFTEKHFVTSQVKKKIMTSIST
                     G"
     gene            complement(10920..12377)
                     /locus_tag="KM925_RS15950"
                     /old_locus_tag="KM925_15945"
     CDS             complement(10920..12377)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055364.1"
                     /locus_tag="KM925_RS15950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15945"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_098239217.1"
                     /transl_table=11
                     /translation="MRTAIVGGGIGGLITALLLSKRGFSVDIFEKENKLGGRLAFVEKD
                     GYKIDEGPTIVLLPLMLERILDEAGISASQYELIRCDPLYDIHFPDGTTYTKKATEKDQ
                     LEEISRLFPGEEQNYRRFMTELKKRFELGSKAFLEKTFVNKRDFWTWQNIKALKKMNAN
                     KSMRKFVSTYFHDERLVDAYSLQSLYIGGNPYSAPAIYSLVPYSEHAHGIYYVKGGYAS
                     LVPLLEKALIEQGVNIHLNKAVQELSISGDKTEGVIVDEKLYPFDQVVLNGDFPNMEKI
                     ARKKEKKYVPSSSCLLLYLGLKRKYDHHNVHQYFMGNDLAKHMEAVFKHKTIPEDPAIY
                     TFNPSVIDPSLAPEGKSVLYALVPVPSGGHIDWSNQQDFVDGIIRTLEEKGFEELRKEI
                     EWVKVRTPNDAQAFGLYEGGSFGIAPELFQSGVFRPQYQPYEYKNVFAVGASIHPGGGV
                     PIVMQGAKMLADHLIEQSSVQTVAKLS"
     gene            complement(12374..13906)
                     /locus_tag="KM925_RS15955"
                     /old_locus_tag="KM925_15950"
     CDS             complement(12374..13906)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055363.1"
                     /locus_tag="KM925_RS15955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15950"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_029324694.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.1e-07, bitscore:
                     27.2, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="DAO (E-value: 9.3e-09, bitscore: 31.1,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQKNVIVIGAGPGGLAAAMLLASHGYKVDVYEKQGYVGGRNSAIK
                     MSGYTFDMGPTFLSMPQIFEELFQAANRNAHDYMDLKRLNLMYELIFDDAQIQMTDNHK
                     KMKEEIERLYPGNGEGYERFMKDTEAKMAALLPLLQGEMDRFHHYLKPEAIRALPHLSL
                     GKTLYDVLGKYFTDEKLKLAFTFQSKYLGMSPWECPGAFSILSFMEHAYGIFHPIGGVN
                     QLSQAMAKVVEEHQGHIHLNQGVQKLWIENKRVKGIILENGERIGADDVIINGDFAHAM
                     TNLIDEGTLKKYAVKKLEKKKYSCSTFMIYLGIDKQYKDLPHHTIVFAEDYKKNVEEIT
                     KTLALSEDPSIYIQNPGVTDPTLAPEGKSALYILAPVPNNFSGVNWDEKQKDFRDAVLK
                     IVEKKTGFKDLESHIEVEKIISPKQWEEDVLVYKGATFNLGHQLTQMMVLRPHNKFEEL
                     ENCWLVGGGTHPGSGLPTILESSRITTKLILKRDGQLLNEPFKTMKEMESAL"
     gene            complement(14222..16069)
                     /gene="asnB"
                     /locus_tag="KM925_RS15960"
                     /old_locus_tag="KM925_15955"
     CDS             complement(14222..16069)
                     /EC_number="6.3.5.4"
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="asnB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333991.1"
                     /locus_tag="KM925_RS15960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15955"
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="WP_013055362.1"
                     /sec_met_domain="GATase_7 (E-value: 3.8e-46, bitscore:
                     151.9, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 6.6e-95, bitscore:
                     314.6, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCGITGWVDLKRSLLNETATITKMADTLSKRGPDDTNVWTQQHVA
                     FGHKRLVVVDPQGGRQPMTREKNEQRYTLCYNGELYNTEDIRKELLKRGYQFQGHSDTE
                     VLLTSYIEWKESCVDHFNGIFAFSIWDEANEKLFIARDRMGVKPLFYRSYEGGIQFASE
                     LKAILAHPEVKAEVGLDGLAEVFGLGPSRSPGHGVFKEINELRPAHALTLTRNGLKIWR
                     YWNVKSEQHTDDLGETTEKVKFLVQDAIVRQLVSDVPVCTFLSGGVDSSAITAIAANAF
                     KESGKGSLHTYSIDYEDNDKFFKANEFQPNSDGTYIKLMSDTFQTNHHRCIISTEDLVQ
                     HLTEAVEVRDLPGMADVDSSLLWFCREIKQDFVVSLSGECADEIFGGYPWFHRKHDLSS
                     DTFPWMRSLDARIDLLQSSWRKKLQLEDYVRARYEETLKEVPELEGESLEDAQRRKLFY
                     LNQIWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEMKMTGNKEKGILRKA
                     LEGILPHDVLYRKKSPYPKTHNPAYTKAVTVWLQSLLQDKQSPLHEFFDKQQLKHIIDT
                     GGEGFKAPWFGQLMTGPQLLAHLAQIHVWFTHNNIQIVE"
     gene            16241..16801
                     /locus_tag="KM925_RS15965"
                     /old_locus_tag="KM925_15960"
     CDS             16241..16801
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461634.1"
                     /locus_tag="KM925_RS15965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15960"
                     /product="DUF2777 domain-containing protein"
                     /protein_id="WP_013055361.1"
                     /transl_table=11
                     /translation="MNHYSKQQALNNQPRSFVIGTVECVDSQWIFFEDESDEASMLHEV
                     MDDTLELLIDTRWEKAIMVDDRQIKLHDGTYLLRNGDKLRLTKKLPYAYEQLLQSLPKE
                     TFLSFTQHLNSLKFSLYDCIYCHNTLCFLPTDGDTKGANFIIYDNEEKICAVQHLFERG
                     THHLDRFEYTVNDGERSMNLFLD"
ORIGIN
        1 tcgtatttgc ccagctgaac cagtacagcc atccaatcat aaagcctgta gaaggtccta
       61 aaaaccttgt agcgtattct tgaaaggaac ctgaaaccgg cattgctacc gctagctctc
      121 ctaaacaaag cataactaag tacatgagga agccgcctat tatatatgca agaatggctc
      181 ctcccggccc tgcctggcta atagtaaaac ccgaacctaa aaataggcct gttccaatca
      241 cgcctcctaa cgcaatcata aataaatgtc tgcttttcat tgttctctgt aattggtttt
      301 gttctgtatg tctattcatc ggtacctccg ttcttcattt tcttatatgt ggtttgtaca
      361 agcatgccta ctttttagca tctgaacagt tgtttcttga attaacttag gaagaactcc
      421 gaacgaataa ggcttataca ctcgctctgt ccacttatgt ccgtctttcc catatactcc
      481 tatattgatg gacggaatat ttaactctcg aatttcacgg aaaggaatcg cccctgcaat
      541 cccccacccc ggaaagttac gtgtaaaagc atcgatttcc tcatctgtct catgaagaga
      601 taaaaagctg ccatccgcta ggtagggaaa aaatttttta aactgaaacg tctctcccgt
      661 ctgttttcca accttctcca cagctgcttg caagggataa agaagctgct gatctctact
      721 ttcgtctttc ttcaagtgat tatgaggcaa gtaaggcggt gcaaaaaaga caattacgcg
      781 cggactttga tgcggatcta acttttgaag ttcttcaatt actttaaagc accttgttct
      841 aagttctaaa tgcgcatact gttcgaaagc tcgttcaata caagcctgag catttatccc
      901 tttttgatcc aattcttcca ggtaatcttc caaactcaca acatccactt tccaagacaa
      961 gtttcgcttt ggtaaattcg ttctccgaac aaaaagttcg taataatcag aaagtttttt
     1021 ctccactttt tcacatgctt cgattgataa tccaattaat tgactcatca cttcttttgc
     1081 cgttcgttca taaatgaaat agttaaaata caaatgactg cttacagcag tttgcacatt
     1141 ataagcctct ttattatctc gttgatacag acaggaaggt ggcagtacca gctctccttc
     1201 aatattttca gctagattaa tattgttatg gatgcttccc gtaatctcag atgcaatcaa
     1261 attaggatct ataccagcaa gcgtatctcc tacgtgtacc tcccttccgt aaatataaaa
     1321 acatggaagc agtttcccag cggctcctgt ataaatatag cgcgtggaat ctccatcgta
     1381 aagaggtgta ataaagtcgg tattaatcgc tgctacatat gaaagatttt tttctttttt
     1441 caaacggttc agttcagaaa cagcagaaat aattccagca tgctgacttt cctcatctgg
     1501 gttgaccata aaaaggacgt ttccttctaa tttatttgga tgcttagtaa aatgcagcac
     1561 attggctaaa tgtacagcaa ttccactttg catatcaacg gctcctcttc caaacatcca
     1621 ttcacctgag cgagcatctc gctgtacatc ttcattaatg ttatgcatag agaaatagcg
     1681 ctgaagttct tctgggtcta atgctatgtc ttttaatgga ccaaaatctt caatgccaac
     1741 cgtatctaag tgagcgtgat aaatcattgt ttgagcattt tttttgctgc cttgaataaa
     1801 agcaaataca ttttttctac ctagcgtatc gccattaatt ttctgttccc aaacgtgaag
     1861 gggattttct ttgaaataag gaaaactttg aagcgtgttt ttaatgaaat cagcaatctt
     1921 tacttcgccg cttgtcccat tgatactttt gattttcact aacgcttttg ttaaacattc
     1981 aacttgctct tccatgctta gctgtgagag ttggatatac acttctattc ctcctgccta
     2041 tcaaataaaa acgtcattta gctgcatatg aataaaaata gtacgatata tacatatcgt
     2101 actattttta tttattttaa accgattact cagcaaatat tttttcaatt cgttcaattg
     2161 cccagtctaa ctcttctttt gaaatcgtta gcggcggagc aaaacgaata accgtactgt
     2221 gcgtttcttt acaaagcaaa ccttggtctt ttaatttttc acaataagga cgagcttctt
     2281 cgtgtaattc taccccgatg aatagacctc ggccgcgcac ttctttaata actggattgc
     2341 ttaagctttg gagcttctgc ttgaaataag aacctaattc taatgaacgc gctggaagtt
     2401 cttcatcttc aattacttcc aaagcagcta atgatacggc acaagcaagc gggtttcctc
     2461 caaatgttga accatgagag ccagggttaa agacacttaa aatgtcttcg ttagcgacta
     2521 cgcaagaaat agggaaaact cctcctccaa gcgctttacc taaaatcagc atatctggtt
     2581 ctacgccttc ccaatcacaa gcaaacattt tgcctgtacg cgctaagcca acttggattt
     2641 catcagcaat aaatagcaca ttatgttctt tacataaatc agctgcagct tttaagaagc
     2701 cttctggagg taaaacgata cctgcttcac cttgaattgg ctcaattaaa aatgcagctg
     2761 tatttggtgt aatagcagct tttaacgctt ctaaatcgcc ataaggaatg gtttgaagcc
     2821 ctggaagcat cggtccaaaa ccacgttgat attctgcttc agacgataag gaaacagctg
     2881 tcattgtacg accgtggaag ttgcccacac atgcgatgat ttgtgcttga ttttcagcaa
     2941 cgcctttcac atcgtaagcc cagcggcgtg cagctttaat ggctgtttcc accgcttctg
     3001 ctcccgtatt cattggaaga gccatatttt tatttgttaa tttacaaatc ttttcatacc
     3061 atggaccaag ctgatcatta tgaaacgctc gtgacgttaa cgttacttta tcagcttgac
     3121 gttttaatgc ttcaataatt ttcggatgac gatgtccttg gttaacagcg gaataagcac
     3181 ttagcatatc catatattta ttattttctg gatcatgtac ccatacgcct tctgcctttt
     3241 caattacaat cggcagcgga tgataattgt gtgccccaaa tttttctgtt tgttcaatga
     3301 ttttcaccgt tttttgtgtt tcagttgtca tatttactcc tccttatttt ccaattagaa
     3361 tgtttcagat gttgtttttg cttgcatgtg aagaacaaga taatccggtc cgcctgcttt
     3421 tgaatccgtt cctgacatat taaatccgcc aaaaggctgg taaccaacaa tcgctcccgt
     3481 acatcctctg ttaaaataca gatttccaac atggaactct tcacgagcgc gctcaatatg
     3541 ctcgcggttg tttgataata aagcgcctgt taatgcatat tctgtattgt ttgcaatctc
     3601 catcatatga tcaaaatctc gcgctttaga gaatgctact accggaccaa aaatttcttc
     3661 ttgcatgaga cgagcttttt catctacatc cgcaaaaata gttggctgaa taaagtatcc
     3721 agttgaattg tctgcttctc ctcccgcaac aagccgtcct tcttcttttc caatttcaat
     3781 atacgataat actttgttat acgacgcctc gtgaataaca ggtcccatat aagttgaaac
     3841 ttcttccggg gttccaacag ttaatgtttt tgttagcgca actgcttttt caagaacttc
     3901 atcatacaca tcttgatgca caacagcacg cgaaccagcg gagcactttt gccctgcaaa
     3961 accaaatgca gagtatacga ttgaagaagc agctaaatca agatccgcat ctttgtctac
     4021 aaccaccgta tcttttcccc ccatttcagc aataacgcgt tttagccatt tttggcccgg
     4081 ctgtactttt gctgctcttt cgtaaatgcg gcaacctact gcgcgcgagc cggtaaatga
     4141 aacaaagcgt gtttttggat gttcaactaa gtagtcaccg atttcaggtg tagcaccagg
     4201 aataaagttt aatacgcctt tcggtaaacc tgcttcttcc attacttcta caaatttcgc
     4261 tgctactaca ggagtggcat cagctggctt taataaaatc gtgttgcctg ttacgatagc
     4321 tgctactgct gttccagcca tgatagctaa cgggaagtta aatggtgaaa tgataatacc
     4381 tacgccaagc ggaatataat tgtactgatt atactcaccg tctcgactct gtactggaga
     4441 accatctttt aacgtcagga cttgacgtgc atagtactct aaaaaatcaa ttgcttctgc
     4501 tgtatcagca tctgcttcgt tccaaggctt tcctgcttct tttacaagat agctagaaaa
     4561 ttcatgcttt ctgcgacgga tgatggccgc tgcacggaaa agaatatctg ctcgatgttc
     4621 tactttccat tttttccacg tttcaaaagc ttttaaagct gactgcatcg cttgctctgc
     4681 tagatcttta tttactgtag agacatatcc aatcacttct tttatattcg ctgggttaac
     4741 agacgtaatt ttctcttcag tttccacttt ttcaccgtcg atcacaagtg ggtacgtttt
     4801 gccaagctgt gtatttacat atgttaatgc ttcttgaaat gcctttttat tgttttcttc
     4861 cttaaaattt gtaaatggtt catgagaata agaaactacc atgatatata cctcctagta
     4921 tgcaaatctt tgtttacttt atagttatgc aaatagcgtg ccaactttta ataaaacttt
     4981 acagcctatt tgtcatcaag gttttcatca cttatatcat tctttctttt tgataaagga
     5041 aaaaataatt ggcatatgta tatttttttt gcactttaaa tgccaatatc tttggcataa
     5101 agaatatatt gagacatacc taaatcaacc caatcaacag ctaagaagca gctacctacg
     5161 tttaccctcc caacgaaaaa atccgaacaa gtttgattct ccatcaagaa tcaaactcgt
     5221 tcggatcttc cttcaactac aatacttttg ttccagcctc ttttctattt gtatataaag
     5281 ttatctcttc gtcatttgaa tagttttaca ggactgtctt ttaatcattt tgtgaggaac
     5341 gcgcacttgt tctgcaacag catatggcgt ttcaatctgc ttaatcaagc agcgtacagc
     5401 ctgataacca agttcaaaaa tattaatatc aatagatgtg agagcaggtc tagccatttc
     5461 ggcgatcaga acattattaa aactgacaat cgaaatgtct tcaggaactg aaatccccat
     5521 ttcatccaat gtatttaaaa cgcctaatgc cattagatcg tctgctacta ccagagccgt
     5581 tggacgttct tttagagata aaagttcgca aacagcttct tttcctccct tctgtagaaa
     5641 ttcttcatga atcacatact cgtcccggta aggaatgttt gctttttgaa tcgctttatc
     5701 atagcctgtt aaacggtcaa tggtaacgac taattcaaga cttccgccaa taaaagcaat
     5761 acgctcatgc ccagattcaa ttaaatattc cgtcgcttct tttccagctt taaaattatc
     5821 attatcaaca aacgtgatgc tttcactatg tttaaacggc tttccaataa ctacaaacgg
     5881 cacgctctgt tcttgcaaat aagaaagtac ttgatcatca actttggagt acagcaaaat
     5941 taatccatcc actcgtctcc cctgtaccat ttgaacaact tctgcaaaaa tctcttcttc
     6001 cgtttctcca gtggacatat aaatcgctct tttgttttcg tgagctccgg tgctgattcc
     6061 tcttagcact tcgggaaaga atggattttg aaacaccttg tctgctgagc tcggcatcac
     6121 taggccgact gcctctgtcg tttgatttgc tagacttcgc gcaataatat ttgggtgata
     6181 accaagttct ttcatagccg atcgcacgcg ctgttttgtt ttttcactaa ttcttgagct
     6241 gtctgcaatg acgcgagata ccgtcgaagg ggctacattt gctaaatttg ccacatcttt
     6301 tattgttaca cccataattc cacctcttaa ttatgttttc ttcattaata cttatccaat
     6361 ataatttgac aactacgacg cgctgtcctt tttacgagat tgagaaattt tcttttcttt
     6421 tcttcttttt gaagatgctt tgtacaaaaa gatgccaaat cccgtataaa cagcagcaac
     6481 cgctgcgata tatccaatat taaaccctga ctttttcact actttatata catttgtggt
     6541 ctcaggctcc gatgaaatgt tgtatacatc gtcaacgggt cgtataattc cttccgttat
     6601 taaccctctt agttcatttt cttttccaac ttttttaagc gggacattaa tgaccgacgt
     6661 ttcctttgta ttattaatca caacaaacgt cgtttcgtcc ttataccttc tcttgtatac
     6721 agccacacct ttttgctcag acacaagggt aaagcttcct tttcgaagag aaggcgactt
     6781 tactctaagt tcagccagtt tttttaaatg atctataata tcgggattag ctaaaaaatt
     6841 catcatgcca taattttctg ctccctttcc cccattcagc gcaatttccg ttccataaaa
     6901 aacgattggt acactcggcg aagtataaag gtaggaaagc ccttgctttg ttcgggctcc
     6961 aggatgattt tcactcttta atgcttgtgc tgtaaaacgt gtgctatgaa catcatctaa
     7021 aaaattggct aaaggctgat tacttctttt cccccacttg tcatacaaca ttttctgcgg
     7081 acgattagta ccgctaaacg tagcagcagt ggcttcggct agcgttctat ctacaagcaa
     7141 atctactcct aacgaatcag cctgctcatt tttttttaat ggttgcccgt ttatttctcc
     7201 tattaataga aaagaaggat ccatgctttt tagcgtctta gaaaaggatg aaataaatgc
     7261 agaaggagct tgatcgatat ctttaacgta atatccgtca atatctgctt gtttgctcca
     7321 ccaagcagca tttttaataa ggtatttctg cacagcactt tcttttaaat taaggtgagg
     7381 caatgcttta attacttcgt tatctgtacc ggattcatta cttatccatg tttgttttcc
     7441 tttatctgct acccaggtat gattgttact gattgtcaaa ggaaattcga ccataatttt
     7501 tatcttgcgc tcatgtgctt tcttcacaag attttgtaag tcttttaacg ttccaaaatg
     7561 atcgtctatt ttataatggt tggtcaccgc atatccatga tagcctgtct catcattttc
     7621 cataaaagga gttaataaaa cagcggtaaa tcccatctct tgaatatagt ctaacttttg
     7681 tgctattccc tgtaaatcgc ctccataata ggcatctgag cgttcatagt taacatttcg
     7741 atcattctgt tcacttccgt tataaaaacg gttgatcatc aatgagtata ttacctcatc
     7801 acgtatatct ttatgatctt gcgctgaggc actgcttcca aaaagcaata atagtataag
     7861 tagcatactt gtccttttcc atttcatcta ttcccctcct atagacgaac tgtttcaaac
     7921 tcacttttgt ttccattata acgttaacgc ttacattatt gttaaataag ttgataaaaa
     7981 caaaaaaagt gattaaaagt ttttactttt aatcactttt ttccatctat atactgctat
     8041 accgtctcat ttacgttcga tatatgaggt ttatccgata ctttaccaac tttatttaat
     8101 cgatcaatca atccaacacc aaattcagga tgaaggttac catctcctgt cgctacgaaa
     8161 ctttcaagaa attcgtatgc ggatgaaccg tgttcagcaa aatctaaacc agcaatttct
     8221 tcttcgcttg atactcgaat agaagaaaaa cgcgtgagaa caaataagaa taacccaacc
     8281 gttccaattg tccagcccat cacagctata attccaagag cttgaatacc taatagctgt
     8341 acgccaccgc cataaaataa accattagct gtatcaaaca gtcccactgc taacgtcccc
     8401 caaataccgc aaattccatg aacagtaata gcaccaactg gatcatctaa cttcattttg
     8461 ctatcgataa aatgcacagc ttctactaga ataattcctg caattagccc aatgacgatc
     8521 gcacctggaa tcgatacgtt tgctgttcca gctgtaatcc ctacaagtcc agctaaggca
     8581 ccgtttaatg ttaagctagc gtcaatacgt ttgtagcgaa actttgtata taaagcagaa
     8641 gacaggactc cgcatgaccc agacattaat gtagttgcga tgattcctgg tacaagggca
     8701 ggatccgctg ctaatgtact tcctccatta aatccaaacc atccaaacca gaggataaat
     8761 acacctaatg cgccaagtag aatattatgt ccttgaatag cattgacttt tccatcgctt
     8821 ccatactttc caagacgtgc tcctagaaaa ataactgcta cagccgcacc aagagctcca
     8881 gtcaaatgaa caactgtcga ccctgcaaaa tctacgaagc ctagtttaga caaccatcct
     8941 cctccccaca cccagtgacc tacaactgga taaataacag ctaccatagc aattgtgagc
     9001 atcatatagc tcgataattt catacgttct gctaccgctc ctgaaataat cgtagcgcac
     9061 gttgctgcga aaacaatttg aaagacaaaa aagctcattt gatcgctttc tccatttaaa
     9121 aagaaaccgc tcgaaccgat aaatcctcct ttagtacttc caaacataag cgcaaaacca
     9181 accacaaaat acaagacgga agacacagct accgttagca tatttttcat taagatatta
     9241 agcgaatttt tcgaacgagt gaagcctgtt tcaaccatcg caaaccctgc gtgcattaaa
     9301 aacacaagta ctgccccaaa cattacccac atcatgtcaa ttgatgcgct gactgattca
     9361 acagttgggt cagccgcatt aacgcgtgtg ctgccaacaa actaataaac aaagccgcac
     9421 taattttctt tttcgacgct taactccccc tctattaaaa taacactctc tttttataaa
     9481 atagcctcta cacctgcttc tcctgttcta attcgtacag catcctctac cggatataca
     9541 aaaatctttc cgtcgcctat ttgatttgta gaacaaattg attgaatgat cttaatgact
     9601 tcatcaactt gttctgtttc taaaatcatt tctaccttga ctttaggtac tagtttgatt
     9661 tcaaaacttt gtcccctgaa cagtccctgc tctccttttt gctgaccgca tcctgctact
     9721 tctgagaccg ttaatccgtt tattcctact tcctctaatt ttacgcgcag tgcttgaaat
     9781 ttttctggac gaatgacggc ttctactttt ttcatcgtaa cccctcttcc atgttagttt
     9841 tattaacatt taatgttatg tatattatca tataggtaat tttctgaata ttcaagttaa
     9901 tatgtgataa aaatgagact tttttcttat ttacataata gttaacgctt tcatatcttt
     9961 tcaaaaaaaa gtaacgcaaa ggaaaagtaa ttcctttgcg ttaccccgta gaaatagatg
    10021 tcataatttt ctttttcact tgacttgtga caaagtgctt ttctgtaaat acgtcataat
    10081 tttcttttcg aattttatca agaatcgctc ggtataaatg cgccgctcct gtaagaggca
    10141 aacgagaagc caccggatat ttatgaatcg actgaagtcc ttttatataa tacatttccg
    10201 cttctttggc caggtgctcc cacatctcta tgaattcaga agtgacccac cggttctcta
    10261 atagcatgcg catatatccg taagaattca taaggtcttt cggtaaataa atgcgccctc
    10321 gctctaagtc ttccccaacg tctcttaaaa tgttggtcag ctgcatcgca atgcctaatg
    10381 caactgcttc ttcttctaat tctttatgag tagttggagc taaaatcggt aaaagcatta
    10441 accctaccgt actcgctacg tgataagagt atgttttcac ttcatcaagc gtatcgtatt
    10501 ctttttttac taaatccatt ctctggccgg taatcatatc atcaaacgct tgaacattca
    10561 tctcatattg ttcaaataca ttgcgcagag ctacccacat ggcgctttcg tttaatagtt
    10621 ctccattttt aaatcgggca aattctgatt cgaattgctg cagctctatt tcagggttgc
    10681 atccttcatc tacaatatca tcgacttgac ggcaaaatgc atatactgcc catactgcct
    10741 ttctttgttt tgacggcagc atggaaaaag ctttgtaaaa cgtttttgag ttttctttaa
    10801 taatttcttc acactgattg tacgctttgt ctaacgaaat gttcatcata taggacccct
    10861 ttctctatta ctttctctat taaaggataa accatcattt ccttatggct tacaagatct
    10921 taagaaagct ttgcaaccgt ttgaacagat gattgttcaa taagatgatc agctaacatt
    10981 tttgctcctt gcataacaat tggcacgcct ccgcctggat gaatagatgc tccgaccgca
    11041 aaaacatttt tatattcgta cggctggtac tggggtctga atacgcccga ttgaaacagc
    11101 tcaggagcaa taccaaagct tcctccttcg taaagtccaa atgcttgagc atcatttggc
    11161 gtgcgtactt ttacccactc tatttcttta cgaagctctt caaacccttt ttcttctaaa
    11221 gttcgaatga ttccatcaac aaaatcttgc tgattgctcc aatcaatgtg gccacctgat
    11281 ggaaccggta caagtgcata tagcacactt ttcccttcag gggctaacga tggatcaata
    11341 acagagggat taaatgtata aatagcggga tcttcaggaa tcgttttatg cttaaacact
    11401 gcttccatat gtttagctaa atcattgccc ataaagtatt gatgcacatt gtgatgatca
    11461 tattttctct tcaaaccaag ataaagcagc agacagctgg acgaaggcac atacttcttt
    11521 tctttttttc gagcaatttt ctccatatta ggaaagtcgc catttaaaac aacttgatca
    11581 aacggataaa gtttttcgtc aacaataacc ccttccgtct tgtctcctga tatagaaagc
    11641 tcttgaaccg ccttattcaa atgaatgttt acaccttgct ctatcagcgc cttttcaaga
    11701 agtggcacaa gacttgcgta tccgcctttt acgtaataga tgccatgagc atgctcactg
    11761 taaggaacaa gggaataaat agctggcgcc gaataaggat ttcctccaat atagagagat
    11821 tgcagggaat aggcatctac tagacgttca tcatggaaat acgttgaaac aaattttctc
    11881 attgatttat ttgcattcat tttttttaat gccttgatgt tttgccatgt ccaaaaatct
    11941 cgtttattta caaatgtttt ttccaaaaat gccttacttc ctaattcaaa tcgctttttt
    12001 aattcagtca taaaacgccg gtagttttgc tcttcgcctg gaaacaaacg gctaatctcc
    12061 tctaactgat ctttttcagt cgcttttttt gtataggtcg taccgtctgg aaaatgaata
    12121 tcataaagtg gatcacagcg tattagttca tattgagaag cagaaatgcc tgcttcatct
    12181 aaaatacgtt caagcattaa aggcagtaag acaatcgtgg gaccttcatc aattttataa
    12241 ccgtcttttt ctacaaacgc caaccggccg ccaagcttgt tttctttttc gaaaatatca
    12301 acagaaaatc ctcgtttgct taataacaaa gcggtaatta aaccgcctat gccgcctcca
    12361 acaattgctg ttctcataat gcactctcca tttccttcat ggttttaaac ggctcattca
    12421 gtaattgtcc gtctcttttt aagatgagct ttgtcgtaat gcgagatgat tcgagaatag
    12481 ttggtagacc gcttcctgga tgagttcctc ctccaactag ccagcagttt tctaattctt
    12541 caaatttatt atgcggacgc agcaccatca tttgagtgag ctgatgtcca aggttaaatg
    12601 tcgctccttt atagaccagc acgtcttctt cccactgctt tggtgaaatg attttttcaa
    12661 cttcaatatg actctctaaa tctttgaaac ccgttttctt ttctacaatt tttaatactg
    12721 catcccgaaa atctttttgt ttttcatccc agtttacacc gctgaaatta tttggaaccg
    12781 gcgctaaaat ataaagagca gactttcctt ctggtgctaa agttgggtct gttacacctg
    12841 ggttttggat gtagatagaa gggtcctctg aaagagctag cgtttttgtg atctcttcta
    12901 catttttttt ataatcttca gcgaacacaa tcgtgtgatg aggcagatct ttgtactgtt
    12961 tgtctatgcc taaataaatc atgaaagtag aacaagagta ttttttcttt tcaagttttt
    13021 taactgcata ttttttaagg gtaccttcat ctattaaatt cgtcatggca tgagcaaaat
    13081 ctccattgat aatgacgtcg tctgccccta ttcgctctcc attttctaga ataattcctt
    13141 ttactctctt attttcaatc catagttttt ggacaccttg gtttaagtgg atgtgccctt
    13201 gatgttcttc cactactttt gccattgctt gagatagttg gtttactcct cctattggat
    13261 ggaagatacc gtaagcgtgt tccataaacg ataaaataga aaaggctcct ggacattccc
    13321 aaggggacat tcccaagtac ttagattgaa aagtaaaagc taatttcaat ttctcatcag
    13381 tgaaatactt gccaagtaca tcatagagcg ttttacctaa ggataaatgc ggcagtgctc
    13441 gaattgcttc aggctttaaa tagtgatgaa aacgatccat ctcgccctgc aataaaggca
    13501 gcaaagccgc catctttgct tcggtgtctt tcataaagcg ctcatagcct tctccgtttc
    13561 ctgggtataa acgttcgatt tcttctttca tctttttatg gttatccgtc atctgaatct
    13621 gcgcatcatc gaaaataagc tcatacatga gatttagacg ttttaagtcc atataatcat
    13681 gcgcattgcg attagcagct tgaaataatt cttcaaaaat ctgtggcatg ctcaaaaatg
    13741 taggacccat atcaaacgta taacctgaca ttttaatcgc tgaatttcgc ccgcccacgt
    13801 atccttgctt ttcatataca tctactttat agccatgact tgctaacagc atcgctgccg
    13861 ctagtcctcc tggacctgct ccaatcacga ttacgttttt ttgcatacat acactcctaa
    13921 tcctatacaa tagttataca aacattatac aaatctttat tattaaacac aacgataaac
    13981 tgcttgtctt tattcgaatc ataaaattac caataactaa accattaaaa catgacgttt
    14041 gtaccatttt tccagggttt cagtacactt tccctttatt cggtgctttc aagcttttat
    14101 tttatatcta tgtaaagtta tacaaaaata gactgcggtc ttcttctcta gttaaacgta
    14161 caaaaaagag aaacagatga cttatttcat aaaaaaaacc ctctgtactg agagggtcct
    14221 gtcattcaac aatttgaata ttattgtgag taaaccagac gtgaatttga gctagatgag
    14281 ctagcagctg aggtcctgtc attaattgac caaaccaagg tgctttaaat ccttctccgc
    14341 ctgtatcaat aatatgcttt aactgctgct tatcaaaaaa ttcatgaaga ggtgactgtt
    14401 tatcttgtaa aagagattga agccatacag ttacagcttt tgtataagct gggttatgcg
    14461 ttttaggata agggcttttc ttgcgataaa gaacatcatg aggcaaaatt ccttcaagcg
    14521 cttttcgtaa aatccccttc tctttattgc ccgtcatttt catctcccac ggaatattcc
    14581 atgcatattc cacaaggcga tgatctgcaa aaggtacgcg tacttctaag ctcgcgccca
    14641 tgctcatacg atctttacga tctaataaag ttgtcataaa ccaaatctga tttaaataaa
    14701 acagctttct tcgctgcgca tcttctaacg attcaccttc taattcaggc acttccttta
    14761 atgtttcttc atatcttgca cgcacataat cttctaactg taatttcttt ctccatgaac
    14821 tttgcagcaa atcaattcga gcatctaatg aacgcatcca tgggaaggta tccgaagaca
    14881 aatcatgttt tcggtgaaac cacggatatc ctccaaaaat ttcatccgca cattctcctg
    14941 ataagctcac aacaaaatct tgtttgattt cacggcaaaa ccaaagcagc gatgaatcta
    15001 catcagccat tccaggaagg tctctcacct ctacagcctc tgttaaatgc tgcacaaggt
    15061 cctccgttga aataatgcag cggtgatgat ttgtttgaaa cgtatccgac atgagcttga
    15121 tataagttcc atcagaattt ggctgaaact catttgcttt aaaaaattta tcattgtctt
    15181 cataatcgat agaatacgta tgaagagagc ccttgccgct ttctttgaac gcatttgcag
    15241 caattgctgt aatggcgctt gaatctactc cacctgataa aaatgtacaa accgggacat
    15301 cggacactag ctgtcttaca atcgcgtctt gcactaaaaa tttaactttt tctgtagttt
    15361 cacctaaatc atctgtatgc tgctcacttt ttacgttcca gtatcgccaa atcttcaacc
    15421 cgttacgcgt aagcgtgagg gcgtgagccg ggcgcaattc atttatttct ttaaatacgc
    15481 cgtggccggg agaacgagaa ggtcctaagc caaacacttc tgctaatcca tctaatccta
    15541 cttcggcttt cacctcagga tgagctaata tcgcttttaa ttccgatgca aattgaattc
    15601 caccttcata agatcgatag aataagggct tcactcccat tctgtcgcgt gctataaata
    15661 acttttcatt ggcttcgtcc cagattgaaa aagcaaagat accgttaaaa tgatctacgc
    15721 atgattcttt ccattctata taagatgtta gaagtacttc cgtatctgaa tgtccctgaa
    15781 attgataacc tctttttagt aattctttcc gaatatcttc cgtgttgtaa agctctccat
    15841 tatagcaaag cgtatagcgc tgttcatttt tttctcttgt catcggctgt cttccgcctt
    15901 gaggatctac tacaactaag cgtttgtgcc caaaagcaac atgctgctgt gtccatacgt
    15961 tggtgtcatc aggacctctt ttcgataaag tgtcagccat ttttgttatt gttgctgttt
    16021 catttaaaag cgatcgcttt aagtcgaccc aacccgttat accacacatc gtttatcatc
    16081 ctttctttca cgctcttgtt tatgaatgaa ataatgccat tgtatgcacg caatagttac
    16141 agttgaattg tccttatatt ttattttcac tttcgagtta taaagagagc ttttttaggc
    16201 aataaaaaaa tatggataaa tttcacacgg aggtttgatt atgaatcatt attctaaaca
    16261 acaagctctg aacaatcagc cgcgttcctt cgtgattggt acggttgaat gtgttgattc
    16321 acagtggatt ttctttgaag atgaaagtga cgaagcttcc atgctccatg aagtaatgga
    16381 tgatacatta gaacttctta ttgatacacg ctgggaaaaa gccattatgg ttgatgaccg
    16441 tcaaataaag cttcacgatg gaacgtattt attgcgaaat ggggataagc taagactgac
    16501 taaaaagcta ccttatgcat acgaacagct tcttcaatca ctaccgaaag agaccttttt
    16561 atcattcact cagcacttaa attcattaaa attctctctt tatgattgta tttactgtca
    16621 caacacatta tgttttttac cgacagacgg tgatacaaaa ggtgctaatt ttatcattta
    16681 tgacaatgag gaaaaaattt gtgccgttca gcatttattc gaacgaggca cgcatcacct
    16741 agatcgattt gaatataccg ttaatgatgg agaacgttca atgaatcttt ttttagatta
    16801 aagcaaaaga aaaaagctat cttatcctga actgcacccc aattgttaga caaataaact
    16861 ctaatgattg gggtgctttt atgtctagac atacatttga agat
//
