LOCUS       NZ_JAHHYI010000003     17426 bp    DNA     linear   CON 30-AUG-2024
DEFINITION  Priestia megaterium strain 179-F 3A1 HS
            NODE_3_length_969567_cov_82.799572, whole genome shotgun sequence.
ACCESSION   NZ_JAHHYI010000003
VERSION     NZ_JAHHYI010000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 969567)
  AUTHORS   Wood,J.M., Hendrickson,R., Singh,N.K., Bateh,J., Peres,J.B.,
            Aronson,H.S., Mason,C.E. and Venkateswaran,K.
  TITLE     Draft Genome Sequences of Sporulating Bacteria Isolated from a
            Spacecraft Assembly Facility
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 969567)
  AUTHORS   Wood,J.M., Hendrickson,R., Singh,N.K., Bateh,J., Peres,J.B.,
            Aronson,H.S., Mason,C.E. and Venkateswaran,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2021) Biotechnology and Planetary Protection
            Group, Jet Propulsion Laboratory, California Institute of
            Technology, 4800 Oak Grove Dr., Pasadena, CA 91109, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-SEP-2020
            Assembly Method                   :: SPAdes v. 3.11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 198.49x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019037625.1-RS_2024_08_30
            Annotation Date                   :: 08/30/2024 04:30:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,420
            CDSs (total)                      :: 6,353
            Genes (coding)                    :: 6,249
            CDSs (with protein)               :: 6,249
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 32 of 104
            Pseudo Genes (incomplete)         :: 71 of 104
            Pseudo Genes (internal stop)      :: 23 of 104
            Pseudo Genes (multiple problems)  :: 19 of 104
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:31:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 811463
            Orig. end                         :: 828889
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHHYI010000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..17426
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="2"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     cand_cluster    1..17426
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..17426
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:12426]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..12426
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     gene            complement(367..1323)
                     /locus_tag="KM925_RS15220"
                     /old_locus_tag="KM925_15215"
     CDS             complement(367..1323)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081827.1"
                     /locus_tag="KM925_RS15220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15215"
                     /product="LLM class oxidoreductase"
                     /protein_id="WP_216884862.1"
                     /transl_table=11
                     /translation="MTTFQHHKAYRRMYKKNELTLGLHMPLENYQYATPTMEKQVELTQ
                     LAEQYGFTSLWFRDVLLQDPSFGDPATGQIYDMLIYLTYLASQTKEIALSTAAIVLPLR
                     HPLRVAKEMATIDQLFPERLIMGVSSGDRQADFRGLGIPHEERGKRFAEAFHYLEEALN
                     KEFPSIQSALGSIQGANVVPKPTKRIPTMITGYSQQNMEWFAQYGDGWMYYPRHPHFQA
                     EAITQWRKLVDIYHPGVFKPFTQPMHLDLAEDPNEMPTPIRLGFRVGRNALVELLSIYK
                     EIGVNHLFFALFDSKRPAEEVIHELGEEVLPHFPSHM"
     gene            complement(1381..2580)
                     /locus_tag="KM925_RS15225"
                     /old_locus_tag="KM925_15220"
     CDS             complement(1381..2580)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461498.1"
                     /locus_tag="KM925_RS15225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15220"
                     /product="MFS transporter"
                     /protein_id="WP_053486854.1"
                     /transl_table=11
                     /translation="MNTLSQTQQSKKSGALPLLALAISAFGIGTTEFVPVGLLSSLADD
                     LNISITLAGLLISGYAMGVAFGAPVLTALTSKMSRKSLLMLLMVIFIVGNSVAALSTSF
                     GLLLIARIITAFSHGIFFSIGATIAADLVPEDKRASAIALMFTGLTVATVTGVPLGTFI
                     GQAFGWRATFWGVALLGVIAIIASALLVPKNLKEAPPAKFSDQLKILRNGPLLLSFAIT
                     ALGYGGTFVAYTYLAPILEKLTGYAPSAVSILLLVYGVAVAIGNTIGGKAANKNPLKAL
                     FWMFLIQAVILVILSFTASFKVAGTITIFAMGLLAFMNVPGLQVLVVNLAEKYVPSAVN
                     VASALNIAAFNVGIAIGSFVGGLIVDSIGLVHTPWIGGVMVLGAVLLTAWSRSIEAKAK
                     "
     gene            2910..3278
                     /locus_tag="KM925_RS15230"
                     /old_locus_tag="KM925_15225"
     CDS             2910..3278
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461499.1"
                     /locus_tag="KM925_RS15230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15225"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_013055500.1"
                     /transl_table=11
                     /translation="MKKYNIPVEAALEVIGGKWKVVILCHLIKGTKRPGELKRIMPRVT
                     QKVLTQQLRELEADGIIVRKMYNEMPPRVEYSLTDYGWSLKSILDSLCSWGEHHIEKTY
                     PDKEAVLMQPVEEVTVSN"
     misc_feature    3285..3300
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.77"
                     /tool="antismash"
     gene            3363..3542
                     /locus_tag="KM925_RS15235"
                     /old_locus_tag="KM925_15230"
     CDS             3363..3542
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055499.1"
                     /locus_tag="KM925_RS15235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15230"
                     /product="hypothetical protein"
                     /protein_id="WP_013055499.1"
                     /transl_table=11
                     /translation="MTEQLKKKISLQDAIKQQLANKKNQSSSKAQNSSTNQATKKLTSQ
                     QTKKTNNQRKRTGV"
     gene            3542..3739
                     /locus_tag="KM925_RS15240"
                     /old_locus_tag="KM925_15235"
     CDS             3542..3739
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055498.1"
                     /locus_tag="KM925_RS15240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15235"
                     /product="YwbE family protein"
                     /protein_id="WP_013055498.1"
                     /transl_table=11
                     /translation="MNGQNRKDVAPGIEVDIVLKADQRSGKLTHGTVKDILTNSSFHPH
                     GIKVRLTDGQVGRIKEIYPN"
     gene            4611..4775
                     /locus_tag="KM925_RS15245"
                     /old_locus_tag="KM925_15240"
     CDS             4611..4775
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461503.1"
                     /locus_tag="KM925_RS15245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15240"
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="WP_078081481.1"
                     /transl_table=11
                     /translation="MKQPQLEKEIRALQSDIYQLAKKTSSYSHGEILKLSQKLDQKIVS
                     YQKLFNRTK"
     misc_feature    4890..4904
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.86"
                     /tool="antismash"
     gene            5001..6680
                     /gene="alsS"
                     /locus_tag="KM925_RS15250"
                     /old_locus_tag="KM925_15245"
     CDS             5001..6680
                     /EC_number="2.2.1.6"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0003984 - acetolactate synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="alsS"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081825.1"
                     /locus_tag="KM925_RS15250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15245"
                     /product="acetolactate synthase AlsS"
                     /protein_id="WP_098562843.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 1.3e-51, bitscore:
                     170.5, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 3e-45, bitscore:
                     149.6, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMSNADNTETKKGADLLVESLASQNVPYIFGIPGAKIDGVFDVLK
                     EKGPEVIVCRHEQNAAFMAAAMGRLTGTPGVCLVTSGPGAANLATGLVTATSEGDPVVA
                     LAGAVPRADRLKKTHQSMDNEGLFKSIAKFSVEVEDAANVPEVITDAFREAVSSPSGAS
                     FVSLPQDVMDNETSASTLSVLKAPTIGAANLDAVAQAIHTIRTAKLPVLLLGMRASKSK
                     AVESIRQLIAEHDLPVVETFQGAGAVSRELETHFFGRVGLFRNQPGDVLLKHADVVLTI
                     GYDPVEYPPSLWNSESQRSIIHLDDLQAQADHHYQPEIELVGDIPSTIDILADKLPHLI
                     RSKENASFLKTLKSKLEQPDDQYEFSNDTHLHPLQLIEALQNFVEDDTTITCDVGSHYI
                     WMARYFRSYEPQHLLFSNGMQTLGVALPWAISAALVRPHKKVLSMSGDGGFLFSAMELE
                     TAVRLNLPIVHIIWNDQTYDMVGFQQQIKYGRKSAVEFGSVDFVTLAESFGAKGFRVNS
                     PEEFSDTLASAFREQGPVIIDVPVDYKDNEKLNTQLLPDQIN"
     gene            6697..7410
                     /gene="budA"
                     /locus_tag="KM925_RS15255"
                     /old_locus_tag="KM925_15250"
     CDS             6697..7410
                     /EC_number="4.1.1.5"
                     /GO_function="GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="budA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016763108.1"
                     /locus_tag="KM925_RS15255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15250"
                     /product="acetolactate decarboxylase"
                     /protein_id="WP_061859576.1"
                     /transl_table=11
                     /translation="MSNKEKLTIHQTSTMIALLDGVFDGVVTYKDLAKKGDFGIGTFNQ
                     LDGEMIAFDGEFYHIADGKAAPISKDAKTPFAIMTTFYEDISYRIEKEMNREQLETLMN
                     ELIPSPNLFYAVRIDGKFKEVKTRTVAKQEKPYPSMVDAAADQPTFTSSDVNGTLAGFY
                     TPEYSSGIGVPGYHLHFIDEYKKEGGHVLDVSVQDVTIQICKKTKLNLNLSETSEFLSA
                     NLEDHDVEEEIATAE"
     gene            complement(7522..7944)
                     /locus_tag="KM925_RS15260"
                     /old_locus_tag="KM925_15255"
     CDS             complement(7522..7944)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461506.1"
                     /locus_tag="KM925_RS15260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15255"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_013055495.1"
                     /transl_table=11
                     /translation="MNQKLAVAVMVVNQDHHVLLVKNHRRGWEFPGGFVEAGESIKDAG
                     IREVKEESGIVIEVTNFLGVEQDVKRSTTVILLKGKAISGKISVSNETQDVGYFTLEEA
                     KNIIKLDNFKDRMARCLKETEIPFFYVLKDTGSFYV"
     gene            complement(7952..9793)
                     /locus_tag="KM925_RS15265"
                     /old_locus_tag="KM925_15260"
     CDS             complement(7952..9793)
                     /EC_number="2.6.1.37"
                     /GO_function="GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_5 (266-542). E-value: 6.8e-30.
                     Score: 96.1. Matches aSDomain:
                     nrpspksdomains_KM925_RS15265_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055494.1"
                     /locus_tag="KM925_RS15265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15260"
                     /product="2-aminoethylphosphonate aminotransferase"
                     /protein_id="WP_053486853.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 1.4e-11, bitscore:
                     40.8, seeds: 297, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 8.1e-25, bitscore:
                     83.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKTAVILAAGMGSRIRKRAGNRPKGFLMIDEKSIIEHSISKLIE
                     AGVKTIFIGTGYMKEEYEKLMVRYPQIKCVYNSRYETTGSLFTLYQFKNYIKEDFLLLE
                     SDVIYEKNALETLVNHSRSDVILASKLNGAGDEVYIEADEDNNLKNMSKQKEELNSIYA
                     ELVGLTKLSYATFQRLCDEANTLFQFNQTIDYEYGLVKISEKANVYVHKLDNLAWCEVD
                     DEDHWARATTIVYPIIKAREGIPHTVKRNILLNPGPATTTDTVKYAQIVEDICPREKQF
                     GETMEFISAELTKFVGNPEKYTTVLFGGSGTAAVESILSSVIDNGTVVIINNGPYGERM
                     CKIAEIYGLNYLEYKSSAEQAIDMNDLKIFIKKISTNVSYLALVHCETSTGLLNDIEQI
                     GEFCAVKNIEMIVDAMSSYAAVPIDMQKMNISYLAASANKNLQGMAGVSFVIANKDRLE
                     KTEQIKPRNLYLHLYDQYRYFQMNKQMRFTPPVQTMYALKQAIIETQWEGIEKRYERYS
                     KSWTTLTDGITRLGLTYLVPETHHSKIITSIIEPSNRKYNFDIMHDFFYKQGFTIYPGK
                     IDKLETFRIANIGDITYRDIERFLHLLEQYLKALKGE"
     aSDomain        complement(8168..8995)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KM925_RS15265_Aminotran_5.1"
                     /evalue="6.80E-30"
                     /label="KM925_RS15265_Aminotran_5.1"
                     /locus_tag="KM925_RS15265"
                     /protein_end="542"
                     /protein_start="266"
                     /score="96.1"
                     /tool="antismash"
                     /translation="YAQIVEDICPREKQFGETMEFISAELTKFVGNPEKYTTVLFGGSG
                     TAAVESILSSVIDNGTVVIINNGPYGERMCKIAEIYGLNYLEYKSSAEQAIDMNDLKIF
                     IKKISTNVSYLALVHCETSTGLLNDIEQIGEFCAVKNIEMIVDAMSSYAAVPIDMQKMN
                     ISYLAASANKNLQGMAGVSFVIANKDRLEKTEQIKPRNLYLHLYDQYRYFQMNKQMRFT
                     PPVQTMYALKQAIIETQWEGIEKRYERYSKSWTTLTDGITRLGLTYLVPETHHS"
     gene            complement(9786..10916)
                     /gene="aepY"
                     /locus_tag="KM925_RS15270"
                     /old_locus_tag="KM925_15265"
     CDS             complement(9786..10916)
                     /EC_number="4.1.1.82"
                     /GO_function="GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0032923 - organic phosphonate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="aepY"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081821.1"
                     /locus_tag="KM925_RS15270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15265"
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="WP_013055493.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 1.1e-09, bitscore:
                     33.9, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 5.5e-14, bitscore:
                     48.1, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISTSVFGTQLKKLGFQFYSGVPCSYLKHLINYAINECEYVAAAN
                     EGEAVAIAAGASIAGNKAVVLMQNSGLANAVSPLTSLIHPFKIPVLGFVSFRGEPGCPD
                     EPQHELMGEITPALLNTMKIKWEYLSFDFAEVKKQLQRANQAVENNESYFFIVKKGTFE
                     PVKLLEKSIKNVTPKMKSEQITTDELPSRYEALQVVNSLKDNDTIQLAATGKTGRELYE
                     IEDARNNFYMVGSMGCVSSIGLGLALSRNEKNIITIDGDGALLMRMGNLATNGYYCPAN
                     LLHILLDNQTHDSTGGQHTVSHNINFVDIASSCGYQNSIHVHNLYELETYIKQWKSDKK
                     LTFLYLKIAKGSKKNLGRPEMKPYEVRERLQVFLDD"
     gene            complement(10909..12426)
                     /gene="aepX"
                     /locus_tag="KM925_RS15275"
                     /old_locus_tag="KM925_15270"
     CDS             complement(10909..12426)
                     /EC_number="5.4.2.9"
                     /GO_function="GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="aepX"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) phosphonates-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055492.1"
                     /locus_tag="KM925_RS15275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15270"
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="WP_013055492.1"
                     /sec_met_domain="phosphonates-like (E-value: 4.3e-138,
                     bitscore: 454.7, seeds: 13, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 1.1e-126, bitscore:
                     417.6, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 1.3e-63, bitscore:
                     210.5, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKTKQLRELIESKQLEFIMEAHSALSAKIVEEAGFKGIWASGLS
                     LSAALGVRDNNEASWTQVLDILEFMSDATSVPILLDGDTGYGNFNNARRLVKKLEQRDI
                     PGVCIEDKLFPKTNSFIKGEAQPLADINEFCGKIKAMKDSQNDEDFVVVSRVEAFIAGW
                     GLKEALKRAEAYRQAGTDAVLIHSKRSDMTEIEAFMKEWGNRLPVIIVPTKYYSVPTDK
                     FSDLGISLSIWANHNLRSSITAMQKTSAQIYCDKSLINVEKNVSPLEEVFRLQKAEELD
                     MAEKLYLSSANHSTNAMILVKESANEALITEQINQLKKVGVTNSIKAGSNKAMEDKAFP
                     NKGELYHLYAARDKLGTATLISDSNIVYKTHVFEELIHESGDITIVAGADYELKGNQTS
                     YVTAKLPYSKQLYSKTVDLIQMSCNPNSNNIHGEFIGLWKVSGKGTEVVKKALEELAQR
                     KDFAQLTFSDFFNYINLIHPVAVKYTMGSWIDTETYTKIQKDGGKND"
     gene            complement(12440..13849)
                     /locus_tag="KM925_RS15280"
                     /old_locus_tag="KM925_15275"
     CDS             complement(12440..13849)
                     /EC_number="6.3.2.10"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055491.1"
                     /locus_tag="KM925_RS15280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15275"
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="WP_061859574.1"
                     /transl_table=11
                     /translation="MKHFTLNKILKQTGGDLCYGSGNPVIKHAINYAKKDIEDHTLIFH
                     LDREPIRGKYWKENHSIVVITDHPELCTNLEGNIILVKTAQLEEAYWKFIEYYRGLFDL
                     PVIGVTGTCGKTTTKEMIRQILIEDYKVKATWMSMNSMSVNLRYLTGIDDTTEVAVFEM
                     PVAYPGYLKVACRYFQPQIRILLNIGVHHLADCETPEEYMKAKAEIVEGLDPNSGILIL
                     NADDENIKRVLDVSHLQGVVYIGKGDDSQFQAKDIKYAKGGMAFTLKYQNKEYDAFIPG
                     YGEHNVYNALAAIAAVFYVGVDISVAIQRLSLFEQVEEHLEFKRGLNECTVINDTWNSS
                     PLSMATALGVLSDMSKEKQSIALLGYMPQLGEGPYAIKQYEDMGKKAAESKIDILVVVG
                     DEAKAIGTGALKCGMDPNKVYFCKTGDEVYNVLAPYLNENTIILLKVTHRVMTKPTFKE
                     LRNKLIPDNEE"
     gene            complement(13846..16467)
                     /locus_tag="KM925_RS15285"
                     /old_locus_tag="KM925_15280"
     CDS             complement(13846..16467)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081818.1"
                     /locus_tag="KM925_RS15285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15280"
                     /product="YheC/YheD family protein"
                     /protein_id="WP_013055490.1"
                     /transl_table=11
                     /translation="MDPMREELGILSDKEMTLQTLNLNNIPSVELVDPKTCSYPVIGRK
                     YGHHSGRDIVIVNTKDQAIYEGYDYFTKIYAIDKEYCLEVEGLSVKTVQVVTSEHVVFN
                     EIPIRTQAFGWKLEQVNGMDVPEMLMNVAIRALYVTGAKSGFVKMGVLENGECIVTDIN
                     SSESEWIENPLKPSVPFSMGADVEFMLSCDGELLPASTFFSVEGPVGCDERQIEQDSGE
                     YALVEVRPEKANSSTELFENIQKLIEKASAQVPYENIHFRAGSMPFSGYQCGGHIHFGI
                     PLSLSLLRALDHYLAIPVALIEESKTAKLRRKTNHGGLGRYREKPYGFEYLTLSSWIID
                     PRITLSTLALAQLVATHHHELKSEFLFHPLTQRAYYQGNKIFLKRMWKDIKANLMKTSS
                     YPHYQNELSFLFEMIEKEIPCDESNDIRRNWNVKISKEIYDRGHIIQIPKKLRLKYGLK
                     EGQSTIVSAGKAISTATVHSYPFSFRHPNMVQLSKSLRDKLSLPKDWCPKLSASEGIIT
                     LGPIIGILANRPFERQTTYFHHLCRLANEKRMLVYVFEPEDIDWEKKLVKGTTINGEGL
                     FPFPAVIYDRYFIDSRKNILIDEVRAKLQAIYKIPFVNSSNLFQLTGDKWATYELLMKE
                     YEEFLPESRLVQKSADIAEMLDSYGEVYLKPLGGALSKGVMRIVRRPTGIFWFDLNKKE
                     LHQFSNMEELFTLLSPLMKNNPYLVQEGIRRKQHKDKNLEIRVYMQKNEKQIWLRTGMV
                     ARLTGEDVLTEDSETNMRLSKILNSLYPDPTDRRLIINQLAKISKNIVATVEEKVGPFG
                     ELAVDLCIDQYGSIKLLEINAKPDSLFSQIRAYKLRTLAGIRLLNYASSLAGYEEEKED
                     LT"
     gene            16713..17387
                     /locus_tag="KM925_RS15290"
                     /old_locus_tag="KM925_15285"
     CDS             16713..17387
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081817.1"
                     /locus_tag="KM925_RS15290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KM925_15285"
                     /product="hypothetical protein"
                     /protein_id="WP_013055489.1"
                     /transl_table=11
                     /translation="MTLKFIHGNYLKYNKGTGFISCHVDALHAEPPAADLFTDKVVENL
                     VKKTGCAGIVSTVSRTIADLNRNLNETNHEGIHEYRRTIQEIVKYLKIADSNNRLLTPY
                     LHLSFHGMKDTHYGPYAIEVGTLYGQSCSKEVKDWFQQALTKKSEDSFPKIHVIFDNKF
                     VGNESIAFHRKGDNSGYLGYSHNFNTFQIELSRTLRSKHRTTITNIFSEIIDDFQQTFV
                     AT"
ORIGIN
        1 gatcaataac tctaatacat atttcttata tatcctataa cttgaaatct atataaaaag
       61 ggattgagac ataacgaaat caatggaatc taaagacgaa ctaattggat aaaggagctc
      121 atatgggtcg cttgttccat cacggttgac ttccgtgcaa gacttcgctt tccgcgggcg
      181 gcccgcgagc ccccccagag tcttcgtctt gtcttccaat caaccgctag aagcaactac
      241 atacataaat gctacgttca tcctaccaat aaaaaaatcc gaacgagtta gattctccat
      301 taagaatctc gattcatcgt tcggatttcc ctttaactaa actacttttg tcccagcctc
      361 tttttgctac atatgagacg gaaaatgcgg aagcacttct tcaccaagct catgtatgac
      421 ttcctcagcc ggacgcttac tatcaaaaag agcaaaaaac aggtgattaa ctcctatttc
      481 tttataaata gaaagaagct caactaatgc attgcgccct actcgaaatc ctaaacgaat
      541 cggcgttggc atttcatttg gatcttctgc caaatctaga tgcataggtt gtgtaaaagg
      601 tttaaaaacg cctggatgat agatgtcaac cagtttcctc cattgtgtaa tagcttctgc
      661 ttgaaaatgg gggtgacgag gataatacat ccagccgtct ccatactgtg caaaccactc
      721 catattttgc tgactgtatc ccgtgatcat cgttggaata cgtttcgttg gcttaggaac
      781 gacatttgct ccttgaatgg atcccaaagc agactggata gatggaaatt ctttatttaa
      841 cgcttcttct aaataatgaa aagcttctgc aaatcgcttt cctctttctt catgaggaat
      901 acctaaccct cgaaaatctg cttgacggtc tccagaagat acgcccataa tcaaacgctc
      961 aggaaaaagc tgatcaatag ttgccatttc tttagctacg cgaagcggat ggcgcaaagg
     1021 cagcacaata gcagctgtac taagagcaat ttcttttgtt tgactggcca gataagtcaa
     1081 ataaataagc atatcataaa tttgacccgt ggctggatcg ccaaatgatg gatcttgaag
     1141 aaggacatcg cggaaccata agcttgtaaa tccatattgt tctgcaagct gcgtgagctc
     1201 tacttgtttc tccatcgtag gcgtggcata ttgataattt tcaagtggca tatgcaaacc
     1261 taacgttaac tcattcttct tgtacatacg acgatatgcc ttatgatgtt gaaatgtagt
     1321 catttgcttc ctctcctttt atagctaaga gatcatcgca aaaggcgatg atctcttgtt
     1381 ttattttgct tttgcttcta ttgagcggct ccatgctgtt aataagaccg ctcctaaaac
     1441 cataactccc ccaatccaag gagtatgaac aagaccaatc gaatcaacaa tcaggccgcc
     1501 tacaaaagaa ccaatagcaa ttccaacgtt aaatgcagca atattaagcg cagaggctac
     1561 gttcactgct gaagggacat acttttcagc taagttcaca accagtactt gcagtccagg
     1621 tacgttcata aatgccaata agcccatggc aaaaatcgta attgttcccg ctactttaaa
     1681 cgatgcagta aacgataaaa taactaagat aactgcttga attaaaaaca tccaaaataa
     1741 tgcttttaac gggtttttat tcgcagcttt tcctccgatc gtatttccga tggctactgc
     1801 tacaccgtat accagtagca agatactaac cgcacttggt gcgtatcctg ttagtttttc
     1861 aagaataggt gctaaatacg tataagctac aaacgttccg ccatatccta aggcggtaat
     1921 agcaaacgat agtaaaagcg gcccgtttct caaaattttc aattgatcac taaactttgc
     1981 tggcggcgct tcctttaagt ttttaggcac taaaagagca cttgcaataa tggcaataac
     2041 cccaagtaac gctactcccc aaaatgttgc tctccagccg aaagcttgtc cgataaatgt
     2101 accaagcgga acacctgtaa cagttgcaac tgtcaaacct gtaaacatta aagcaatggc
     2161 acttgctcgc ttatcttccg gcaccaaatc agcagcgata gtcgcaccga tggaaaagaa
     2221 aattccgtgt gaaaatgcag taataattcg tgctataagc agcagcccaa agcttgttga
     2281 taaagctgct actgagtttc ctacgataaa aataaccatc aatagcatca acagtgattt
     2341 tctgctcatt ttacttgtta aagctgttaa tacgggagct ccaaatgcaa ctcccattgc
     2401 gtatcctgaa attaataatc ctgctagtgt aatggaaata tttaaatcat ctgcaagcga
     2461 tgataataaa cctactggaa caaactctgt agtcccgatt ccaaaggcgc taatagctag
     2521 cgctaatagc ggcaaagcac cgcttttttt actttgttgt gtttgtgaaa gtgtgttcac
     2581 ttcttaacgc ctcctgttcg ttatgataac ttcttcataa tagcttctct tacggctgat
     2641 tagcttaggt acctttcata tattcgctaa ctcgccatca acaattgtta ttatgagcga
     2701 tgtaaaacca aagaaaaagt acgcactttc aagtgatata ggcacctgga ggttacaata
     2761 agcgtaaata ctaaagtcat gaactattgg gtattctaaa aaaatgctta cacctagaaa
     2821 attttgctct atagtgatat gataaacatc agaaaaaatg cgaaaacatg catttgaccg
     2881 ttatctctag aacaaggaga gttgttaacg tgaaaaaata taatattcca gtggaagcgg
     2941 cactagaagt cattggaggc aagtggaaag tcgttattct ttgccactta attaaaggga
     3001 caaagcgccc tggagaatta aaaagaatta tgccgcgcgt tacgcaaaaa gtattaacgc
     3061 agcagcttcg cgaacttgaa gcagacggca tcattgttag aaaaatgtac aatgaaatgc
     3121 cgcctcgagt agaatactct ttaacagatt acggatggtc tttaaaaagc attcttgatt
     3181 cattatgctc ttggggagaa catcatattg aaaaaacgta tcctgataaa gaagctgtac
     3241 ttatgcagcc tgtagaagaa gtcactgttt ctaattaaaa aaagctgact atctagtcag
     3301 ctttttcatt cccttacttt ctttgttttt tcagatgatg tatatattca ggaggaaccc
     3361 atatgacaga acaacttaaa aagaaaatta gtcttcaaga tgctatcaaa cagcagcttg
     3421 ctaataagaa aaatcaatct tcaagtaaag ctcaaaatag cagcaccaat caagcaacca
     3481 aaaagctgac gagtcaacaa acaaaaaaaa cgaataatca gcgaaagaga acaggtgtat
     3541 aatgaacgga caaaatagaa aagacgtagc tcctggaatt gaagtcgaca ttgttttaaa
     3601 agcagatcag cgttctggaa aacttacaca cggaacggtc aaagacattc tgaccaattc
     3661 aagctttcac ccacacggca tcaaagtccg tttaacagat ggacaagtcg gacgcataaa
     3721 agaaatttat ccaaattaaa aaagcatgta ttctacatgc ttttttaatt tacacttcaa
     3781 ataaatctac ttatcatttc ctacttttgt taaaataaag aagcactgtc gtcccctttc
     3841 tttcccttgg gaattcacgc aaggaagaaa aaggtggtgg atagtctgag acgaatcctc
     3901 aagtcatttt tcctagatgc tgagaatgtt cagctcctaa cggcttacgt agagctttta
     3961 ggagccatag tcgctcttat tttgctattt ttccagtagt aaaataagta atcaccgata
     4021 agtatcgtct aaaaggttct tcatccataa ggctcgtgtg ctgattgtct ttagggaatg
     4081 gtgtttggcg gcacttccct atagtaaaaa gacagcttcc ttgcagaaat ggacaaggtg
     4141 taatcaacta cggccgatta tgggtgcggg acctttttct ttattagtct atgtcttttt
     4201 ttcacttccc atacacaaaa ataaaaaaac ctccgagttg gcggtctcga aggttttttc
     4261 tagtgaatcg ttttcgacaa atcctcatcc gggctaacaa ggttcagatc taataagatt
     4321 atgtgatgaa tttcttcacc ttacaagcta aacttgtcaa agctaagaat tcattcgctt
     4381 gttaagaata actgaacgtg cgctccagat tgtcattttt cctagcctca gtatatcata
     4441 cttgttgaaa acaggtaaaa aaaagtgttc gacaaagctt gtcagcagct cttcacctat
     4501 gtcatatttt tcaaaacaaa aaacatttta caaatatgaa ggaatttcat actattagat
     4561 aaaagataca acatagaaga tagaagtatt ctgacttgga gaggatttca atgaagcaac
     4621 ctcagttaga aaaagaaatt cgagccttac aatcagatat ttaccaatta gctaaaaaaa
     4681 caagcagcta ttctcacggt gagattttaa aattaagtca aaagctagat caaaaaattg
     4741 ttagttacca aaagcttttt aaccgtacca aataagagcc gcgtaggact ttcacttgct
     4801 atccctcaag taccataaac tacaaataaa acagatatgt tttattcgca ataggtatat
     4861 ctctgcttcc ctaattaata gggaagtttt ttatttagaa aattaaaacc ttctcctttc
     4921 cttaggttag ttgtcataca aattgggtat ccaatcagta ggccttaaaa cctatctaag
     4981 aaatataaag gagagagtgc atgatgagta atgctgataa taccgaaaca aaaaaaggcg
     5041 ccgacttact agtagaaagt ttggcaagtc agaatgtgcc ttatattttt ggcattccag
     5101 gagccaaaat tgatggcgta tttgatgttt tgaaagaaaa aggaccagaa gtaattgttt
     5161 gccgccatga acaaaatgct gcgtttatgg cagctgctat gggaagactg acaggtacgc
     5221 cgggcgtttg ccttgtcaca tcaggtccag gtgccgctaa cttagccact ggccttgtca
     5281 ctgctacttc tgaaggtgat ccagtggtag ccctagcagg agccgttcca agagcagatc
     5341 gattaaaaaa gacgcatcaa tcaatggata atgaaggact atttaaatct atcgccaaat
     5401 tcagcgttga ggtagaagac gctgcaaacg ttccagaagt gataacagac gcctttcgcg
     5461 aagctgtttc ttctccttca ggagcatctt ttgtcagctt gccgcaggat gtaatggata
     5521 acgaaacatc agcttccact ctatccgttt taaaagctcc tacgattggc gctgctaatt
     5581 tagacgctgt tgcccaagct atacacacta ttcgcacagc aaaactacct gttttacttt
     5641 taggtatgag agcaagtaaa tctaaagcag tggaatcaat ccgtcagctt atagcagaac
     5701 atgaccttcc tgtggtggaa acgttccaag gtgcgggagc tgtttcacga gagcttgaaa
     5761 ctcacttttt tgggcgagta ggtctttttc gcaatcagcc tggagacgtg ttattgaagc
     5821 atgctgacgt cgtcttaaca atcggatatg acccagttga ataccctcct tctctttgga
     5881 actcagaaag tcaaagaagc attattcatt tagatgattt acaagcacaa gctgaccacc
     5941 attatcagcc ggaaattgag ttagtagggg atattccaag cacaattgac atattagcgg
     6001 ataaactccc acatttaata cgttcaaaag aaaatgcttc ttttttaaaa acgctaaaaa
     6061 gcaaacttga acaacctgac gatcagtacg agttttctaa tgacactcac cttcatcccc
     6121 tacaattaat tgaagctctg cagaactttg ttgaagatga tactaccatc acatgtgacg
     6181 ttggttcgca ttacatatgg atggctcgtt attttcgttc ctatgaacct cagcatttac
     6241 tattcagcaa cggcatgcag acactaggtg ttgctttgcc ttgggctatt tcagctgcac
     6301 tcgttcgccc ccataaaaaa gttctgtcca tgtctggaga cggggggttt ttattttcag
     6361 ctatggagct cgagacagcc gttcggttaa acttaccaat tgttcacatt atctggaacg
     6421 atcaaaccta cgatatggtt ggctttcaac agcagattaa atatggccga aaatctgcag
     6481 tagagtttgg cagtgtagac tttgttacgc ttgctgaaag ttttggtgcc aaaggattta
     6541 gagttaactc accagaagaa ttttcggaca ctctagcaag cgcttttcgt gaacagggcc
     6601 ccgttatcat cgatgttcct gtagattata aagataatga aaaattaaat acacagctac
     6661 tgccagatca aattaactga aaggactgag taaagaatga gcaataaaga aaaattaacg
     6721 attcaccaaa cttccactat gatcgcttta ttagacggag tatttgacgg tgttgtcaca
     6781 tacaaggatt tagctaagaa aggggatttc ggcattggta cctttaatca attggatggt
     6841 gaaatgattg cctttgacgg agaattttat catattgctg atggtaaagc cgcacctatt
     6901 agtaaagatg caaaaacacc ttttgccatt atgacaacct tttatgaaga tatctcttat
     6961 cgtatagaaa aggaaatgaa tcgtgaacaa ctagaaacct taatgaacga gcttattcct
     7021 agcccgaatt tattctatgc agttcgcatt gacggcaagt ttaaagaagt aaaaacgcgc
     7081 acggttgcaa agcaagagaa gccctatcct tctatggtag atgcggctgc tgatcagcct
     7141 acatttacat cttcagatgt aaatggaaca ctagcaggtt tttacacgcc agaatattct
     7201 tcaggcatcg gtgttcccgg ctaccacctg cactttattg atgaatataa aaaagaaggg
     7261 gggcatgttt tagatgtctc cgttcaggat gttacaattc aaatctgcaa aaaaacgaaa
     7321 ttaaatctca atttatcaga aacaagtgaa tttttaagtg ctaatttaga agaccatgat
     7381 gtagaagaag aaattgccac ggctgaataa acaaccatac ttttactgat aaaaaaacgc
     7441 tcatcgtcac gcatgacgat gagcgttttt tatttaatcg cgttcaggct tcatttctag
     7501 cacaacacag aaaccttccg cctatacgta aaaggagccc gtgtctttta aaacatagaa
     7561 aaatggtatt tcagtctctt ttagacatct agccattcga tccttaaagt tatctaattt
     7621 aattatattt tttgcttctt ctaacgtaaa atatcccaca tcctgcgttt cattggagac
     7681 agaaattttt ccgctaatcg cttttccctt taataaaata acagtagtag atcgtttaac
     7741 atcttgttct actcctagaa agttggtcac ttcaataaca attccagatt cttctttaac
     7801 ttctcgtatc cccgcatctt ttattgattc acctgcttca acaaaaccgc ctggaaattc
     7861 ccatccccgt ctatggttct tgactagaag cacgtgatga tcttgattta ccaccataac
     7921 tgctacagct aatttttgat tcaacttttt ctcactctcc ttttaaagct ttaagatatt
     7981 gttcaagaag gtgaagaaat ctttctatat ctctatatgt gatatcacca atatttgcta
     8041 ttcgaaacgt ttctaattta tcaattttac caggataaat ggtgaatcct tgtttataaa
     8101 aaaaatcatg cataatatca aaattatact ttctattgga aggctcgatg atagaggtaa
     8161 taattttaga atgatgtgtt tcaggaacca aataggttaa cccaagccga gtaattccat
     8221 ccgttaaagt agtccatgat tttgagtagc gttcatatcg tttctcaatt ccctcccact
     8281 gagtttctat aattgcctgc tttaaggcgt acatagtctg aacaggagga gtaaaacgca
     8341 tttgtttatt catttggaaa tatcgatact gatcatataa atgaagataa aggttacgag
     8401 gctttatttg ctccgttttt tctaaccggt ctttatttgc tataacaaac gacacgcctg
     8461 ccattccttg aaggttctta tttgcacttg cggccagata gcttatattc attttttgca
     8521 tgtcaattgg aacagctgca tacgaactca ttgcatccac aatcatttcg atatttttca
     8581 ctgcacaaaa ttccccaatt tgttcaatat cattaagaag ccccgtggaa gtttcacagt
     8641 gaacaagcgc cagataggaa acgttcgttg aaatcttttt tataaaaatt tttaagtcgt
     8701 tcatatcaat agcctgctcg gcggagcttt tatattctaa ataattcagt ccataaattt
     8761 cagcgatttt acacattctt tcaccgtatg gaccattatt gataataaca accgtaccgt
     8821 tatcaattac tgaacttaaa atcgattcca ctgccgccgt tccagatccg ccgaataata
     8881 ccgtagtata cttctctgga ttcccaacaa attttgttag ttcagcagag ataaattcca
     8941 ttgtctctcc aaattgtttt tctctcgggc aaatgtcctc cactatttga gcatatttga
     9001 cggtatctgt tgtagttgcc ggtccggggt ttaggagtat atttctttta actgtatgtg
     9061 gaataccttc tcgagcttta ataatcggat aaacaatagt agttgctctt gcccagtgat
     9121 cttcatcatc cacttcacac catgccaaat tatccagttt atgaacatac acatttgctt
     9181 tttcggaaat tttaacgagg ccatattcat aatcgatcgt ttgattaaac tgaaacagcg
     9241 tatttgcttc atcacaaagt ctctgaaatg tagcataaga gagtttggtt aaaccaacta
     9301 gttctgcata gatactattc aactcttctt tttgctttga catattctta aggttattat
     9361 cttcatcagc ttcaatataa acttcatcgc cagcaccgtt caatttactc gctagtatga
     9421 catctgatct cgaatgattt actaatgttt ccaacgcatt cttttcataa attacatccg
     9481 attccaatag taaaaaatct tctttaatat aatttttaaa ttgatacagc gtaaacagac
     9541 ttcccgttgt ttcgtatcta gaattataaa cacattttat ttgaggatat ctaaccatta
     9601 gcttttcata ttcttctttc atataacctg ttccaataaa aatcgtcttg actcctgctt
     9661 ctattaactt ggaaatggaa tgttcaataa tcgatttttc atcaatcatc aaaaaacctt
     9721 tagggcggtt tccagctctt ttacgaatcc tgctccccat ccctgcagct agaataacag
     9781 ctgttttaat catctaaaaa cacctgcagt ctttctctga cttcataagg cttcatttca
     9841 ggacgcccca ggttcttttt agatcctttc gctattttaa gatataaaaa cgttaatttt
     9901 ttatcacttt tccactgttt gatatacgtc tctaactcat ataagttatg aacatgaatg
     9961 gaattttgat acccacagga agaagcaata tctacaaaat taatgttatg agagaccgta
    10021 tgttgtcccc ctgtagagtc atgggtttga ttgtctaaaa gaatgtgtaa taaatttgct
    10081 ggacagtagt atccgttggt tgctagattt cccatgcgca taagcaacgc tccgtctcca
    10141 tcaattgtaa tgatgttctt ttcatttctg ctcaaagcta agcctaaacc aatggaacta
    10201 acgcacccca tagatccaac catataaaaa ttatttcttg cgtcttctat ttcgtaaagc
    10261 tctcttcctg tttttcctgt agcggccaat tgaatagtat cgttgtcttt taatgaatta
    10321 actacttgta atgcctcata ccttgaaggt aactcgtcgg ttgttatctg ttcacttttc
    10381 attttaggag taacgttttt tattgatttt tctaaaagct taactggctc aaacgttcct
    10441 ttttttacaa taaagaagta gctttcatta ttttcaacag cttgattagc ccgctgtaat
    10501 tgttttttaa cttccgcaaa atcaaaagat aaatattccc attttatttt catcgtattt
    10561 agtagggcag gagtaatttc tcccattaat tcatgctgtg gttcatctgg acatccaggt
    10621 tctccgcgaa aactgacaaa tccaaggaca ggaattttaa acggatgaat aagagaggtt
    10681 aaaggggaaa cagcatttgc taatccggag ttctgcatta aaacaaccgc tttgttgcca
    10741 gcaattgatg ccccagcagc aatcgctacc gcttctcctt cgtttgctgc tgcaacatat
    10801 tcacattcgt tgatagcgta gttaataaga tgtttaagat aggaacaagg aaccccgcta
    10861 taaaattgaa aacctagttt ttttaattga gttccaaata cggacgtact aatcattttt
    10921 cccaccatct ttttgaatct ttgtatacgt ttcggtatct atccatgacc ccatcgtata
    10981 tttaacagca actgggtgaa ttagattaat ataattaaag aaatcagaaa acgtgagctg
    11041 agcaaaatct tttctttgtg ccagttcttc caaagctttt tttacaacct cagtgccttt
    11101 tccagaaact ttccaaagac caatgaattc accatgaatg ttgttagagt ttggattgca
    11161 cgacatttga atcaagtcta ctgttttaga atacagctgc ttggaatagg gaagtttagc
    11221 agttacataa gatgtttgat tcccttttaa ctcataatca gctcctgcta cgatagtgat
    11281 atcgccactt tcatgaatta actcctcaaa aacatgtgtt ttatatacga tgttactatc
    11341 gcttattaaa gtagccgttc ctagcttatc tcttgcagca tagagatgat ataattctcc
    11401 tttattagga aacgccttat cttccatagc tttgttggaa cctgctttta tactatttgt
    11461 cacgccgact ttttttaact gatttatctg ctcggttatc aaagcttcat ttgctgattc
    11521 ttttaccaga atcatagcgt tcgtgctatg attggcagag cttaaataaa gtttctctgc
    11581 catatcgagc tcttcagctt tttgaagacg gaaaacttcc tcaagcggag agacattttt
    11641 ttctacatta atcaaacttt tatcgcaata aatctgagcg gacgtttttt gcatagctgt
    11701 aatggaagat cttaagttat ggttggccca aatgctcaag ctaattccta aatcactaaa
    11761 tttatcagtt ggaaccgaat aatatttggt tggaacaata ataaccggaa gcctgtttcc
    11821 ccactctttc ataaaagctt caatctctgt catatctgac cttttactat gaatcaaaac
    11881 ggcgtcggtt cctgcttggc gataagcttc tgcacgcttt aaagcttcct tcagccccca
    11941 ccctgcaata aaagcttcta cacgcgatac cactacaaaa tcttcgtcat tttggctatc
    12001 tttcattgct ttaattttgc cgcaaaattc attaatatct gcaagcggct gtgcttcacc
    12061 tttaataaat gagtttgttt ttggaaacaa tttatcttca atacatacgc ctggaatgtc
    12121 tcgctgctcc aatttcttaa ctaatcttct tgcattatta aagttcccat agcctgtatc
    12181 accatcaaga agaattggaa cggacgttgc gtcactcata aactctaaga tgtctaacac
    12241 ctgtgtccat gaagcctcgt tattatctct tacacccagt gctgcagata atgacaggcc
    12301 gctggcccat atccctttga accctgcttc ttctacaatc tttgcagata gcgcactgtg
    12361 agcctccata ataaactcta gctgtttact ttctatcaac tctcttagct gtttagtctt
    12421 cttcatgtct atcctccttt tactcttcgt tgtctggaat aagtttattt cttaattctt
    12481 taaaagtagg ttttgtcata acacggtgcg ttacttttaa taaaattatt gtattttcat
    12541 ttaaatatgg agctagcaca ttgtatactt catcaccagt cttgcaaaaa tagactttat
    12601 tagggtccat tccacatttt aaagctcctg ttccaatagc ttttgcttca tctcctacta
    12661 caactagaat gtcaattttg gattccgctg ctttttttcc catatcttca tattgtttaa
    12721 ttgcatatgg tccttctcct agttgaggca tgtagcctaa taaagcgata gactgtttct
    12781 cttttgacat atcactaaga actccaagag ctgtagccat cgataaaggt gaagagttcc
    12841 atgtgtcgtt aattaccgta cattcattca gtcctctttt aaattccaaa tgttcttcta
    12901 cttgttcaaa taagctaagt ctttgtatgg caacggaaat atcaacgccc acataaaata
    12961 ctgcagcaat ggcagccagt gcattataca cattatgttc accatatcca gggataaagg
    13021 catcgtactc tttgttttgg tactttaaag tgaaagccat tcctccctta gcatatttaa
    13081 tatcttttgc ttgaaattgg gaatcatcac ctttacctat atatacaaca ccttgaagat
    13141 gactaacatc aagcactctc ttaatatttt catcatctgc gtttaaaatc aaaatgccgc
    13201 tatttggatc taagccttct acaatttcgg cttttgcttt catatattct tcaggtgttt
    13261 cacaatcagc gagatggtgc acgcctatat ttaatagaat tcgtatttgt ggctgaaagt
    13321 agcgacacgc aacttttaaa tagcccggat aagcgaccgg catttcaaaa acagctactt
    13381 ctgtcgtatc atcaatacct gttaaataac gtagatttac agacatagaa ttcatactca
    13441 tccacgtagc tttcacttta taatcttcaa ttaatatttg tcggatcatc tctttcgtgg
    13501 tcgtttttcc acaagttcct gtaacaccaa taacaggaag atcaaaaagc cctcggtagt
    13561 attcgatgaa tttccaataa gcctcttcta attgagctgt tttaacaaga ataatatttc
    13621 cctctaagtt cgtgcacagc tcagggtgat ctgttataac gacaattgaa tgattttctt
    13681 tccaatactt ccctctaatc ggttctctat ctaaatgaaa gatgagtgta tgatcctcta
    13741 tatccttttt agcgtagttt atagcatgtt taataacagg atttcctgat ccataacaaa
    13801 ggtctccccc cgtttgttta aggattttat ttaatgtgaa gtgcttcatg ttaaatcctc
    13861 cttttcttct tcgtagccag ccagtgaaga cgcataattg agcaggcgaa tccccgccaa
    13921 agtacgcagc ttgtaggctc taatttgtga gaatagacta tctggttttg catttatttc
    13981 aagtaacttt atggaaccgt actgatctat acataaatct accgccagct ccccaaaagg
    14041 acccactttc tcctctactg ttgcaacaat atttttagat attttagcta attggtttat
    14101 aatgagcctt ctgtctgttg gatcaggata taaactgttt aatattttac tgaggcgcat
    14161 attcgtttca gaatcttccg ttagcacatc ttcaccggtg agacgcgcaa ccatacccgt
    14221 tcgaagccaa atttgctttt catttttttg catataaacc cgtatttcta aatttttatc
    14281 tttatgctgt tttctgcgaa ttccttcttg aacaagataa ggattgtttt tcatcaaagg
    14341 agagagcaaa gtaaataatt cttccatatt actgaattga tgcagctctt ttttattaag
    14401 atcaaaccaa aagatgcctg ttggtcggcg aacgatacgc atgactcctt tacttaaggc
    14461 cccgccaagc ggttttaaat agacttctcc gtagctatca agcatttctg caatgtcagc
    14521 agatttttgt acgagacgtg actctggaag aaattcttca tattctttca taagaagttc
    14581 atatgttgcc cacttatctc ccgtgagctg aaacaaattt gaagaattaa caaaaggaat
    14641 tttataaatc gcctgaagtt ttgcccttac ttcatctatt aaaatatttt ttctactatc
    14701 tatgaaataa cggtcataga taacagcagg aaaaggaaaa agcccttcac cgttaatggt
    14761 tgttccttta accaattttt tctcccaatc aatatcttcg ggttcaaaca catatacaag
    14821 cattcgcttt tcatttgcta agcgacatag atgatggaaa taggttgttt gtctttcaaa
    14881 aggacggtta gcaagaatgc caatgatagg acctaaagtt atgatgcctt ctgaagctga
    14941 tagcttgggg caccaatctt tcgggagaga tagtttatca cggagagatt tagagagttg
    15001 aaccatattt ggatggcgaa atgaaaaagg ataagaatga acagttgcag ttgatattgc
    15061 ctttcctgca ctaacaatcg tagactgccc ctctttgagg ccgtatttta aacggagctt
    15121 tttggggatt tggataatat gcccgcggtc atagatttct ttagaaatct tcacattcca
    15181 attcctgcga atatcatttg attcatcaca aggaatttct ttttctatca tttcaaatag
    15241 aaaagatagt tcattttgat agtgtggata gctggatgtt ttcataagat tggctttgat
    15301 atctttccac attcgtttaa gaaagatttt attcccttga tagtaggcac gctgcgtaag
    15361 tggatgaaat aaaaattcgc tttttaattc atgatgatgg gtagcaacta gctgagcaag
    15421 agctaaagtg gagagagtaa ttctagggtc gataatccaa gagcttaaag ttaaatactc
    15481 aaatccatac ggtttctcac gatagcgtcc aagacctcca tggttcgttt tccgtctgag
    15541 tttagcggtc ttagattctt ctatcagcgc tactggaata gcaaggtagt ggtcaagagc
    15601 tcttaataag gataaagaaa gcggaatacc aaaatgaata tgtcctccac attgataccc
    15661 tgaaaacggc atgctgcctg cccgaaaatg aatattttca tatggtacct gtgcagatgc
    15721 tttttctatt aacttttgga tattctcaaa tagttctgtt gatgaatttg ctttttcagg
    15781 cctaacttct actaatgcat actcaccgct atcttgctct atttgtcttt catcacagcc
    15841 taccggacct tcaacagaaa aaaaagtaga agctggtaga agctctccat cacaacttaa
    15901 cataaactca acgtcagcac ccatgctaaa aggaacgcta ggctttaatg gattttctat
    15961 ccattcactt tctgatgagt taatatctgt gacgatacac tctccatttt ctaatacccc
    16021 cattttgaca aagccgcttt tagctcccgt tacgtagagc gctcgaatgg ctacgttcat
    16081 cagcatttcc ggtacatcca tgccgtttac ctgttctaat ttccagccga aggcttgcgt
    16141 tcgaatgggt atttcattaa atacaacatg ttcactggtc actacttgta ctgtttttac
    16201 gctcagtcct tctacttcta gacaatattc tttgtctatt gcatatattt tcgtaaaata
    16261 gtcatatcct tcatatattg cttgatcctt tgtatttacg ataacaatat ctcttccgct
    16321 atggtgacca tacttcctcc ctatcactgg atatgaacag gttttggggt caactaattc
    16381 aacagacggt atattattca gattaagagt ttgcaacgtc atttctttat cagataatat
    16441 ccctaactct tctctcattg gatccatttt aaagcctcct aattagaact ttctatcgta
    16501 ttttgctacc ttaatgccct ataacaataa tcagctactc atatagtatg taactattca
    16561 aaataatcct gtacatttat ctatttatct ccaccatctc taaaaacttt ttatgtatgt
    16621 atttaacacg tgtaataatt caccgatttt atttttactg catttattat agaaaggata
    16681 gatttcaaac gatataaaag gagaaatagt caatgacatt aaaattcatt catggaaact
    16741 acttaaaata caacaaagga actggtttta taagctgtca tgtggatgca cttcatgcag
    16801 aaccacctgc tgctgattta tttacggata aagttgtaga aaatttagtc aagaaaacag
    16861 gatgtgcagg aatcgttagt acggtttcca gaaccatagc agatttaaat agaaatctaa
    16921 atgaaacaaa ccatgagggg atacatgaat atagaagaac aatccaagaa attgttaaat
    16981 atttaaaaat agcagatagt aataatcgcc ttctaacgcc ctatttgcat ttatcttttc
    17041 atggaatgaa ggatacacat tacggacctt atgcaattga agtggggact ttatatggac
    17101 aatcttgctc aaaggaagtt aaagattggt ttcaacaggc gctgactaaa aaatctgaag
    17161 attcgttccc gaaaatccat gtaatatttg ataataaatt tgttggaaac gaatcaattg
    17221 cctttcacag aaaaggcgac aacagcggct atttaggtta cagtcataat tttaatacat
    17281 ttcaaattga attatcccgg accctgcgca gcaaacacag aactactata acaaatatat
    17341 tttccgagat tattgatgac tttcaacaaa cattcgtcgc tacttagttt ttaggaatct
    17401 tttccgtcta ctatttcttt tatata
//
