LOCUS       NZ_CP075608            10375 bp    DNA     circular CON 12-SEP-2024
DEFINITION  Enterococcus faecalis strain 1207/14 plasmid p1207_4, complete
            sequence.
ACCESSION   NZ_CP075608
VERSION     NZ_CP075608.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21607)
  AUTHORS   Wardal,E., Sadowy,E., Zabicka,D. and Hryniewicz,W.
  TITLE     VanA Enterococcus faecalis in Poland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21607)
  AUTHORS   Wardal,E., Sadowy,E., Zabicka,D. and Hryniewicz,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) Molecular Microbiology, National Medicines
            Institute, Chelmska 30/34, Warsaw 00-725, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018986755.2-RS_2024_09_12
            Annotation Date                   :: 09/12/2024 05:12:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,549
            CDSs (total)                      :: 3,463
            Genes (coding)                    :: 3,375
            CDSs (with protein)               :: 3,375
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 35 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 19 of 88
            Pseudo Genes (multiple problems)  :: 9 of 88
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:43:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9686
            Orig. end                         :: 20061
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075608.1.
            Bacteria and source DNA available from National Medicines
            Institute, Poland.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10375
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10375
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10375
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5375](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5375
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(626..641)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 20.88"
                     /tool="antismash"
     gene            1843..2754
                     /locus_tag="KKP64_RS17385"
                     /old_locus_tag="KKP64_17395"
     CDS             1843..2754
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1157: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237640.1"
                     /locus_tag="KKP64_RS17385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_17395"
                     /product="ParA family protein"
                     /protein_id="WP_002333825.1"
                     /transl_table=11
                     /translation="MELSQENLKIVFEYRNVVRRPLTITVANSKGGVGKTTIIRYLSYV
                     LANMGFKVLVVDADPQSNTTKTMLLTKNYHSKDDDIFIIEKTMLAGIVERDLKQLVLPI
                     IENLDCIPSHIDFKNFPKYLTKLYGDAIEGIDADYIEVEKNRISVLKDLLEPVKPEYDF
                     VLIDTPPTMSDFTRNAAYASDYIIMAFQTQSDSLDGVEDYISEEVTPLVEKFDCKTEIV
                     GILPNQLSKGSIDRQVVNDAIEKFGDQNLFDNILPHVKRVQTTPRTGLNTDTYWDKLAY
                     EEVFVPLAKDFIHRIKLFEGEN"
     gene            2756..3127
                     /locus_tag="KKP64_RS17390"
                     /old_locus_tag="KKP64_17400"
     CDS             2756..3127
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629602.1"
                     /locus_tag="KKP64_RS17390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_17400"
                     /product="hypothetical protein"
                     /protein_id="WP_002333824.1"
                     /transl_table=11
                     /translation="MTDNAKPGFFSKQKPDNSYKEIPKPKSAFTSDNTDLLENIHPEED
                     VLKINNKSLGIPAIVWCEFTALLDTTDNDYTYELLEELIHERTARFDHNQKTTYNRILE
                     RKKEQERERIYKKEKRKKI"
     gene            3390..4070
                     /locus_tag="KKP64_RS17395"
                     /old_locus_tag="KKP64_17405"
     CDS             3390..4070
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /locus_tag="KKP64_RS17395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_17405"
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /transl_table=11
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
     gene            complement(4407..4868)
                     /locus_tag="KKP64_RS17400"
                     /old_locus_tag="KKP64_17410"
     CDS             complement(4407..4868)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /locus_tag="KKP64_RS17400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_17410"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /transl_table=11
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            5001..5375
                     /locus_tag="KKP64_RS17405"
                     /old_locus_tag="KKP64_17415"
     CDS             5001..5375
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /locus_tag="KKP64_RS17405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_17415"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 3.2e-09,
                     bitscore: 28.1, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
     gene            5429..7555
                     /locus_tag="KKP64_RS17410"
                     /old_locus_tag="KKP64_17420"
     CDS             5429..7555
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /locus_tag="KKP64_RS17410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_17420"
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /transl_table=11
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
     gene            7552..8172
                     /locus_tag="KKP64_RS17415"
                     /old_locus_tag="KKP64_17425"
     CDS             7552..8172
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /locus_tag="KKP64_RS17415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_17425"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /transl_table=11
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     misc_feature    8264..8280
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.69"
                     /tool="antismash"
     gene            8286..8423
                     /locus_tag="KKP64_RS17420"
                     /old_locus_tag="KKP64_17430"
     CDS             8286..8423
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /locus_tag="KKP64_RS17420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_17430"
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /transl_table=11
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
     misc_feature    8692..8711
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 19.45"
                     /tool="antismash"
     gene            8865..9245
                     /gene="mobC"
                     /locus_tag="KKP64_RS17425"
                     /old_locus_tag="KKP64_17435"
     CDS             8865..9245
                     /codon_start=1
                     /gene="mobC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /locus_tag="KKP64_RS17425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_17435"
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002319809.1"
                     /transl_table=11
                     /translation="MSEQNQNLASNYSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
     gene            9227..10141
                     /locus_tag="KKP64_RS17430"
                     /old_locus_tag="KKP64_17440"
     CDS             9227..10141
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /locus_tag="KKP64_RS17430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_17440"
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /transl_table=11
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
     misc_feature    complement(10197..10214)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.75"
                     /tool="antismash"
ORIGIN
        1 ccagcacgaa cacgaaaatg aattttgttc tcttctctta actgcttcag tactttgtcc
       61 agggaacgtt ttgggatctt taaacgttca gttaactctt tttttgttga aataatgtat
      121 ggttgttctt cataggattg ctcattcaaa taggacagta gatcttctgc ccattcataa
      181 ctatgtgaat acttgcgaac agctctttct ttcttgaatt tccaccaacc ccgtttttta
      241 ttgaatagct gctcttcaga aagattggca tcgacccact cttggcataa taggacaact
      301 ttttctctgt gcgctgcttg atattttccg gagtaagcgg aacgaatgat tctggttaat
      361 tctcgatcag ataaagggtt agccaagcgg tcattgaatt ctaacatcgt caattcacat
      421 gcttctactt cgtatcctga cgaaaagtat gccaaagaca aggtgaatat gacattattt
      481 cgaccatata cgcccttttc accttgtatt ttttgacttt ttagcaacag ctggaaccag
      541 ggttcatcga cttgacgaaa ttcttgagta gatggtaata caaaaacgtt cttgatctcc
      601 gcttttttgt ccgcatcttc tctcattgac cagtcgatcc attcttcgaa actataacga
      661 tactgtggtt caaagaataa aacattctct tcagaaggca ttcttgtgat cccaaaatga
      721 ttggcattta catcgacagg aaaccgataa tcatttgaaa atttattcct tagatttttt
      781 gaaatcattt tggccacatt gatcacttta aaatttgatt tcttcgtcac atatgcaggc
      841 ttgtctagta catagaaagc ttgaaatcca tgcggtgtac gtagaatcat tgtcggcata
      901 aagccgatct cgtgactggc caagataatt tctccatagt caaagtttgg atccaatgtg
      961 tcaaaatcaa caaagaatgt attgatttga cgtaaattgt cctccgaatg acctttcgta
     1021 aacattctgg cttcatcagc atacgttcca tagcgaaata catttggtgt ccaatgagtg
     1081 aaacgattat tgttttcgat caccccttct tctgaagtga tcactagtcc aatcccctcg
     1141 atcatgtgtt cctttgaacg gtacgcaaaa atagcccctt tccctgactg atcactaaaa
     1201 gagaccggtt ttaggtggct gtttttatat ttgaatttac ggataccatt ttgtaatacc
     1261 gtcgaatata cgacatccaa agaagctgcc atgactgtta ttccttccca aactaaaaga
     1321 gaacacaaat aatcttaaag atttgtgttc tcccaatagc ttagaagtct taaatgaatg
     1381 acaaaacaag catgtatata ttgagttgct cgtcaaaaaa tggtagaata aatctgtaag
     1441 ttcggattgt cagtccgaaa gacttatcaa aattacataa gtggttgtca gccacttaca
     1501 taacttctga gacgagcgag aacacatagg cttttatgtg attcagatag aatagtgagg
     1561 ccaatttcac ttttttatcc gtctcttttt tttatgtaat tttcttttaa agtgtaaact
     1621 tttttttaat gctgtaaacc tttttttata ctacaaagtt accctatttt taaacatttg
     1681 taaagattat caagtgactt gattataaat tatattttat aaactataat ttataaagta
     1741 taattgacaa catatttcct tctggtatag tgttcttaga cagatatata tattatattt
     1801 tgcaatttat attttatatt ttataaacgg gaggtttgtt taatggaatt atcgcaagaa
     1861 aacttgaaaa ttgtttttga atatagaaat gtcgtcagaa gaccattaac tattactgta
     1921 gcaaattcca agggtggagt aggtaaaact actattataa gatacttgag ctacgtactt
     1981 gctaatatgg gatttaaagt gttggtagtg gatgccgatc cgcaatcaaa tactacaaag
     2041 acgatgttgt tgactaaaaa ttatcactct aaagacgatg acattttcat tattgaaaaa
     2101 actatgctag ctgggatcgt tgaacgagat ttaaaacaat tagttcttcc aattattgaa
     2161 aacttggatt gcattccatc acatatagat tttaaaaact ttccaaaata tttaaccaag
     2221 ctttatgggg acgctataga aggaatagac gctgattata tcgaggtcga aaaaaacaga
     2281 atctccgtct tgaaagattt acttgagcca gtcaagccgg aatacgattt tgttctcatc
     2341 gatactccac ctactatgtc tgatttcact aggaatgctg cttacgcttc tgactatatt
     2401 attatggctt ttcaaaccca atctgatagc ttagatggcg ttgaagatta tatatcagaa
     2461 gaagtcactc ctcttgttga aaaatttgat tgtaagactg aaatagttgg aatacttcca
     2521 aatcagttga gtaaaggtag tatagatcgc caagttgtca atgatgctat cgaaaagttt
     2581 ggtgatcaaa acctttttga taatatttta ccacatgtta agcgagtgca aacaacaccg
     2641 aggacaggtt taaatactga cacttattgg gacaaacttg cttacgaaga agtttttgta
     2701 cctttagcaa aagatttcat tcatagaata aagttattcg aaggagaaaa ctaacatgac
     2761 cgataatgca aaaccaggtt tcttttcaaa gcaaaaacct gataattctt ataaagaaat
     2821 ccctaaaccg aaatcagcat ttacttctga caacacagat ttactagaaa atattcaccc
     2881 tgaagaagac gtattgaaga taaacaacaa gtcgcttggt ataccagcga tagtctggtg
     2941 cgaatttact gctttgcttg atactactga taatgattat acctatgaac tactcgaaga
     3001 attaattcac gaaagaactg cacgtttcga tcacaatcaa aaaacaacct acaaccgaat
     3061 attagagcgt aagaaagagc aggaaagaga acggatatat aagaaagaaa aaaggaagaa
     3121 gatataaatc ttcttccttt ttatattttg taaagtacaa tttataattt atattttgca
     3181 aattataaat tgcaacaaaa attaagcctt taaatacaat taattactcg ctggcaaagg
     3241 ttttataaat attactttat aatttatatt ctatatttta taatttataa atataaccag
     3301 ccgtgttctc ttgacggttc tgttgcaaag ttttaaatct actatcaaat aaggtagaat
     3361 aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag caatttcagc
     3421 aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc tatcgtgaag
     3481 ttcaagaaat cttatatgat cgtggcatta acgtttctca tacgacgatt tatcgttggg
     3541 tgcaagaata tggcaaacta ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt
     3601 attcatggaa aatggatgaa acgtacatca aaattaaagg aaaatggcat tatttgtatc
     3661 gagccatcga tgcagatggt ttaaccttgg atatttggtt acgtaaaaaa cgggacacac
     3721 aagcagccta tgcttttctt aagcggttag tgaagcagtt tgatgaaccg aaggttgtag
     3781 tcacagataa agccccctct attacaagtg cctttaagaa actaaaagaa tacggctttt
     3841 atcaagggac agaacatcgt accattaaat acctgaataa tttgattgaa caagaccatc
     3901 gtccagtaaa gagacgcaat aaattctatc gaagtttacg cactgcctct accacgatta
     3961 aaggcatgga agccattcga ggattatata agaaaacccg aaaagaaggc actctcttcg
     4021 ggttttcggt ctgtactgaa atcaaggtat tattgggaat cccagcttaa atcatagata
     4081 ccgtaaggga ttttattctt tatttaaaac tttgcaacag aaccagatta tcctagttta
     4141 gcaacataaa ccatttatta gatgaaaatg agatacaaac aaagcgtatc aaatcctctc
     4201 aaaaataaca agtctttaaa aaatgacaga taaaaatgca caaagaatta gatatagata
     4261 accgcgaaaa atcaagcctt aaaaagagct ttttacctag accaattaaa aatcacaaag
     4321 tgagcccctg tatcggttga tacaagggct acttggcata ctgtacatta tgtcaactat
     4381 gcaattaaat aactaactac ttcatattag cttaataaaa taatcaaaat aactaggggg
     4441 aaaaacattg tataaaaaaa tttttcattt tttaaataag aatagtaaat ataaaaataa
     4501 tttaagatat ctattactat taatccagat agagcaaacc aagtaaatat tccatgtgta
     4561 aagctaaaaa aattcatttg aatcaattca aataaaccta atatagtaac aaatatatta
     4621 aaaataatag ctgtcaatac cagtaaatta ttttttttat tttttgaatt atcttcattt
     4681 aaaatagaat ttatgttagt actataaaat tcacaaattt tttttagtgt ctctatatct
     4741 ggtgttgttt tgttattttc ccactttgaa atagtttgac gtgtaacgtt aaattttctt
     4801 gccacatatt cttgagtata cccttttttc actcttaatt ctcttagctt agatcctaaa
     4861 tcctccattt tttctcctat gtaaacgatt atttacataa aattttatca taaaaatact
     4921 aacgcgccaa aaaagttctt tattttataa aacacactaa aataaaataa ttctagtaaa
     4981 aaagatagga gaaataagtt atgaaaaaat ttttatgtct ttctgcttta atgggtgtct
     5041 tattaactag tggaggaata gctagtgcca cagaagcttt aaatctagat gtacccgaag
     5101 accataccgc catttacgga ggaggaatgg aagctacaga agaaggattt tcttctagta
     5161 aattgagata tgctgctggc ggcggggatt tcaactgtgg ggttaatgga tttaaagttt
     5221 atgcaaatta ctaccatgct agagctaaac atagtgcaac agctaaaaat ggacgaggcg
     5281 gtcaagtacg atcagttcag aaagctggag taagagctta tgcaacatgc aatgccacac
     5341 ttactggaaa tactggttgg tggaatgttt attaaaaaag agagtttaaa ctctcttttt
     5401 tatttacatt tgaaaggaaa ttcaaaatat gttaaaaaaa ataaacgctg ttttatcact
     5461 ttttgtctgc attacaatac tatttttttc tactaatgtt ataaatggta taaagacttc
     5521 tgaagcgcta tataaaaata ccgctgaaat tcatatgcaa tataaagatg atatggactt
     5581 taacagttat ataaacgctt tgtatgaaat atctacacaa gaaaatatta atatttctca
     5641 atatagtttt actgctgtta accaactaac tattcttgct acaaacccta acgctaacaa
     5701 acactggaaa ttttctaaaa aaaatatatt agcaagaaac gattctaaag tccattatac
     5761 taataacaaa aattctaagt accaattaaa acttccaaat aattttttaa atataaatat
     5821 ttatcctttt tctcatgtgg aaaatattgg attaagtgaa atattatatg tacaaggaaa
     5881 ctcaaacaaa ttaattcctg tactgaaaaa atatggaaca cctactattt ccaatatcaa
     5941 tcaatcagat acttttcaaa ttaatactaa tattgttctt gttatctgct atcttagtat
     6001 atttattgta attactacaa ttatatttgc tttttcaaaa atgaaagaaa tcactctaaa
     6061 aaaaatgtta ggctataact cttttgatac tataaaagat tctattaaag agttaaacct
     6121 cttattttgt tctttgatta taactttcat tttatctatt tttttgagca tatattacag
     6181 taacgaaaat tttttcctat catttcttct atgtagtatt tttcatttta taattagttt
     6241 tttatttatc atagttactc taagtatcta tcatttatct agtataaaaa attatctaaa
     6301 aaattcacga ccacttaagt taaatctttg tatattaaat atctgtctat tcttaagcat
     6361 gatccttctg ttaatagctt ctacaaaagt aataaatacc cataatgaag ccgaaaataa
     6421 ttctcttaaa tattgggaaa gaactactaa tttatacaaa acaaatatta ctaatcaatt
     6481 aaatagaaac aacaccgtag aagaaaataa ctacttgaaa aaagctagta aatttgttta
     6541 taaaatacag aagaattata aaacttttat tatcgcgcca tacaattacg caactatcca
     6601 agagaataat aaagaatttt ttattggaca aaaagtttat ccaaattttg aagactatgt
     6661 tagtcaacct gctggtgcag gaatatgtgt tgatttaaac tatctaaaat attacaatcc
     6721 tatttctttt gaagaatcta ctgacttaaa tttaataaac tctgatccac taactactta
     6781 cattctagta cctaaaaaat ataaagaata tgcaaaaatg attgaaaaaa attatttaga
     6841 agacatacag tttagacttg aggactctcc tcctcaagct ccttataaaa atcctaattt
     6901 gaaaaattta aaaataaaac taatatttgt taataataac caaaagtatt ttagttttaa
     6961 taccttatat ggaaaggcaa aagataacta tacaataact gatcccatag ttagagttat
     7021 taatccagaa ataacagaat cattattttg gggaaatata ttgactagtg atggtggatt
     7081 attttttgat cagaaacata cttcaaacca aaactttttt aatcaaatta atccgtatat
     7141 aaaagaattt aacctagaaa atatcataaa tttttcttat tctgtttttc aagaaactgg
     7201 tgaattatta tctcaaaaaa aagtaaataa attaaatgca tatctacaat atcttttaat
     7261 attaggatta tttataataa ttaattttca aatgattttt gttagtttta ctataaattc
     7321 aaaaaaaata gctttaaaaa aaatatttgg atattcacca ataagtcaag ttttagattt
     7381 atgtttactc ccttttacaa tagagttatt tgctactatg tgttttcaat tgatattttc
     7441 aaaaaacact tacttgtatt ttataatttt tatgatcttt ttaataaatc taattacttc
     7501 tattattatt tataaaaaaa atagttttaa atattttaag gagaatattt tatgatatta
     7561 tcagttgaga atttcaccct gtcaattaat aaaaaaaaga tcaaacaaaa ttggaacttt
     7621 tcaatatcag aaaaagattt tattatagta acaggaaaaa gtggtagtgg aaaaactact
     7681 ctgttaaata atttaagcct aatagataaa caatataccg gtaacttatt ctattttgaa
     7741 aagaaagttt ctaaacatga tatacaacgt ctacgaagaa atcatattag ctatttattt
     7801 caaaactatg ctcttcttga aaatcaaaca gtacaataca atttcgaatt agcaaaaaag
     7861 tttaacagta atatttccaa tgatcatatt tataatttat taacagattt ttctttgcca
     7921 aaagatatct taaaagaaaa aatctttctc ttaagcggtg gagaacaaca aagaatagca
     7981 ttaattagaa atatgttaaa accatttgat atattatttg cagatgaacc tacaggaaat
     8041 ttagataaag aaaactcaac ttttataatg aactttctga aaaatttaac agaaaaagaa
     8101 aaaaaagccg ttgtactagt aacacatgac ttaaatgttc taaactatgg aactaatatt
     8161 atagaattat aatttttacc cgtgatgcta gctgatttaa aactagcacc acgggtattt
     8221 attttatatt ccatctctcc tatcccaaaa tttaaataat cacttaataa aaaggaggca
     8281 cccaagtgat tttattgaaa aaagagtatg aaaaatttta tgatagactt atttattctt
     8341 tgcaattgcc caaaaaatta aaccgtcaat tacaaaagac ttatgaaact aaatcaactg
     8401 aagaatggga gagttattgc tagatttttg gtagaagtaa caaaaagtgg acatattatc
     8461 ataatgactc gccgaaagtc tgataaaaaa ttggctaaaa atattgctga atgtttaata
     8521 aaactatcag ttttagctaa atagttagta gtttacacaa aaaagcattt atgttttttg
     8581 ttgcaaagca acaatttcat cttcggtgaa ataagcgaaa ctttacaaaa agtggctagc
     8641 atttgctagc tttttttgta ttgtggagcg tggcttttga gggggattca agggggagct
     8701 tgcgcccctt tgacaagcgg ttatttgcac taaatacgta ggtatttggc tagcaaatat
     8761 agagcttgcc aaaccctttt agtgagcttt actcattgta cctttttagg tacaatggta
     8821 ttgcgaacga tacgtccgta ggacgtactg aaagggtggg acttatgagc gaacaaaatc
     8881 aaaatttggc tagcaattat tctaaaaaat atacctatcg atccgagcca aaacaaatca
     8941 gttttcgagt gagcgaatcc gaatttgcaa agctgaagca atcggctgaa gctttgcaaa
     9001 tgagtgtgcc ggctttcgtg aaagccaagg cacaaaacgc gcgtctggtg acaccgaaag
     9061 tggcgccaga cattgcccag gcaatcgcgc gcgatttagc caaagctgga gggaatatca
     9121 atcaaattgc taagtggtgc aatacacacc aacatgacgt tgcttctggt gacgctcaac
     9181 gtttatccga aaacctaaaa atcatgcaaa aggagctaca aaagatatgg caacaattaa
     9241 ggtaagccga accacttctt gtagtcgtgc aattaattac gcagaaccac gagcaaccat
     9301 taaaacagga ataaactgtg acgtcgatta tgccaaaagt gaaatgaaac aaattcgcat
     9361 gctgtatgga aaagacgacc gtgtacaggc acacttattg attcaatctt ttcgaccagg
     9421 agaaattacc gctgaaaaag ctaatcaact aggggcagag tatgcaaaaa aaatagcccc
     9481 cgatcaccaa atagctattt acacacatac agacaaagac catattcata atcacattgt
     9541 cattaactcc gttaatctgg agactggaaa aaaatttcaa gctcatggtc aagcatttct
     9601 aaacaaatgt tatgacctaa atgacgagat ttgtcttgca cataacttac caatcactga
     9661 gcgaggaaag aaaccagaaa aacgaaccat gtctgaaata aaattaaaag aaaaaaatga
     9721 acctgtttgg aaagacgaaa ttcggattgc gatcgatcaa acaatgaaaa acaaaaaaac
     9781 cagaacctat gaccaatttt gtaattccct caaaacattg gggattcatt gcttcaatcg
     9841 cgggaaaaat ttcacttacg aattgatcca caaaaagaaa aaagtacgat ccaacagact
     9901 aggaaaggac tacgaaaagg agacgatttt acatgagctg gacagacgag aacgaaccta
     9961 taaccaacag cgaaccaacg agctacgaag agctcgagct agcattcgcg aaactgcaga
    10021 agaagtacga agagaacaac gaactcttac gcctgaacaa ccggaaatta gaccggctcg
    10081 aagaaaccat gaacaacacg ttgaaagagg aacagaccac caacaagaat tatcgctcta
    10141 aaacgttaaa agacatagaa aacgaaataa agaaacaacg tgcttttctc gttccttata
    10201 cgcaagaaag gatacaaact acgattgatt tacaagttaa aaaactccat caagagctat
    10261 tagaccagcg aaataaggaa cagaaaaagg tgcttactca acgatcattg gattttcttg
    10321 cccccttatg ttgcttagtc ctgacactgt tcgcattttg gttagtatgg ggagg
//
