LOCUS       NZ_CP075604            10882 bp    DNA     circular CON 12-SEP-2024
DEFINITION  Enterococcus faecalis strain 1207/14 chromosome, complete genome.
ACCESSION   NZ_CP075604
VERSION     NZ_CP075604.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3264821)
  AUTHORS   Wardal,E., Sadowy,E., Zabicka,D. and Hryniewicz,W.
  TITLE     VanA Enterococcus faecalis in Poland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3264821)
  AUTHORS   Wardal,E., Sadowy,E., Zabicka,D. and Hryniewicz,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2021) Molecular Microbiology, National Medicines
            Institute, Chelmska 30/34, Warsaw 00-725, Poland
  REMARK    Nucleotide sequence updated by submitter
REFERENCE   3  (bases 1 to 3264821)
  AUTHORS   Wardal,E., Sadowy,E., Zabicka,D. and Hryniewicz,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) Molecular Microbiology, National Medicines
            Institute, Chelmska 30/34, Warsaw 00-725, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018986755.2-RS_2024_09_12
            Annotation Date                   :: 09/12/2024 05:12:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,549
            CDSs (total)                      :: 3,463
            Genes (coding)                    :: 3,375
            CDSs (with protein)               :: 3,375
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 35 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 19 of 88
            Pseudo Genes (multiple problems)  :: 9 of 88
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:43:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1478481
            Orig. end                         :: 1489363
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075604.2.
            On Aug 11, 2021 this sequence version replaced NZ_CP075604.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from National Medicines
            Institute, Poland.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10882
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10882
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10882
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5882](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5882
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    737..748
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.03"
                     /tool="antismash"
     gene            1013..2407
                     /locus_tag="KKP64_RS07305"
                     /old_locus_tag="KKP64_07310"
     CDS             1013..2407
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406369.1"
                     /locus_tag="KKP64_RS07305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07310"
                     /product="anti-CBASS protein Acb1 family protein"
                     /protein_id="WP_216788164.1"
                     /transl_table=11
                     /translation="MGNIANEAKLLKVDGKAYRNDFMIGNGKGYAKDNLSRQRPGISKR
                     LSYSQLEDLYSSNSMAKNIVDIPAEDLTRNGWNLKMKDEQAKALYESKLRQLKTKDRLQ
                     QLFTYERLYGDGFVSIGTIEKSNYSLDKPLNFKNIKSVPYINAFSGKKISNRIINEDVF
                     SPNYGQIESFEINNRSRDARIELLPNSTYSTTTKIHYSRVLHQQNLRFEDELAGSSLLD
                     NLYDILTVVDTSIWSVGQILYDYIFKVYKSSDVSNLTSEEKLEIAMKMDYKFRTEALTI
                     IDSEESLGKESSSVSGIGELLDFVWDYLAGAARMPKTVLKGQEGGTVTGAQYDVMNYYS
                     RITAIQENQLRPHLEYLIRCLMWAEDECNGRLDPDSIEWSIEFNPLWNVDSKTDAEIRK
                     LIAEADKIYIESGVVDPDEIEEARFGRFGVTETSKFNADSLSRDELDKMAAIVYENYKQ
                     DRDNEK"
     gene            2397..3179
                     /locus_tag="KKP64_RS07310"
                     /old_locus_tag="KKP64_07315"
     CDS             2397..3179
                     /GO_component="GO:0044423 - virion component [Evidence
                     IEA]"
                     /GO_process="GO:0016032 - viral process [Evidence IEA]"
                     /GO_process="GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619009.1"
                     /locus_tag="KKP64_RS07310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07315"
                     /product="minor capsid protein"
                     /protein_id="WP_233029141.1"
                     /transl_table=11
                     /translation="MKNNPKTRYPLRLEESYAKNIQKLINELEKVLLYEFDKHLASKID
                     ESRLVNDSRFIQDGLFDTASKLVKKVQTYFLGIFQNRTAQKIVRKHISSVNTFNKSNVN
                     AQLKSRGINPLENETWLNNYMKAKISENVSYITNIRDDYSQKFEQIIYRGVTSAKSSSE
                     IRDELVKLTEMSLKRASFIARDQTGTILGKLNSERQKRAGFSAFRWSDSGDERVRDSHR
                     ERNGKIYFYANNPLLPGEEYNCRCVAEPVDEDSIIEKS"
     gene            3198..3410
                     /locus_tag="KKP64_RS07315"
                     /old_locus_tag="KKP64_07320"
     CDS             3198..3410
                     /GO_function="GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010711277.1"
                     /locus_tag="KKP64_RS07315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07320"
                     /product="LysM domain-containing protein"
                     /protein_id="WP_010711277.1"
                     /transl_table=11
                     /translation="MVKEKKAIDKETKEVKEVLKPNSKTTHTVSKDETASEIATKYHMS
                     LKKLLVINNLDSVKEIKEGVSLIVE"
     misc_feature    complement(3414..3429)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.0"
                     /tool="antismash"
     gene            3422..4528
                     /locus_tag="KKP64_RS07320"
                     /old_locus_tag="KKP64_07325"
     CDS             3422..4528
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417184.1"
                     /locus_tag="KKP64_RS07320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07325"
                     /product="DUF2213 domain-containing protein"
                     /protein_id="WP_216788165.1"
                     /transl_table=11
                     /translation="MVIRYDKAFIQDFKETDEGYLTITACPITRPGVFPYRRTDGGVSM
                     EAKLPDELFSKTTVLSANAKPMTDDHPTEPVTAANYNKYSKGMTHNDAHVLDNKLVVSF
                     TITDVETIKKINDGKRELSIGFQADVSKETGIYNGMQYDSVQRNMLINHIAIVDEGRAG
                     PEVAIRGDSVAFMIDSKDKQTGGNNMSKLIIDSKEFEVDPIVKAKFESLEAKLDAAKQR
                     NTNIEKLEGERDALKAQIDELNKEIAEAKKKEVTGDALDKLVQDRVDLISKAQKFLGDS
                     ADFSGKSDREIKESAIVKISPDFKGDGKSDDYIAAHFDSAVAHIEKKGFTNPAAFNDAK
                     NQDKDKEKEEEIEKQKKARLNMNKKEDK"
     gene            4531..4977
                     /locus_tag="KKP64_RS07325"
                     /old_locus_tag="KKP64_07330"
     CDS             4531..4977
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377545.1"
                     /locus_tag="KKP64_RS07325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07330"
                     /product="hypothetical protein"
                     /protein_id="WP_016619011.1"
                     /transl_table=11
                     /translation="MTIPYPEKYMKPELGVGKLADYQDVQADSLVVGAEGLGFGVGVQV
                     KENVATAYKDGKFYGVSYAKNYVEEIPYGDVEKIGKYKEHEMVPILRKGSIWVKVNEDV
                     LAGEDAKVLNGGNFGKATTSDTVIGTFKTTASSGNLAILQINLP"
     gene            5001..5882
                     /locus_tag="KKP64_RS07330"
                     /old_locus_tag="KKP64_07335"
     CDS             5001..5882
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377543.1"
                     /locus_tag="KKP64_RS07330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07335"
                     /product="DUF2184 domain-containing protein"
                     /protein_id="WP_104669532.1"
                     /sec_met_domain="Linocin_M18 (E-value: 3.8e-49, bitscore:
                     164.7, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNEVTATLEARDLQAIDNVIYQAPQEELVARTLFNVKTDINPGA
                     ETYAYNVMTRSGAAKIIANGADDLPLVDIDMKRYQSPIFTIAAGIRYSRQEIRQAQMMG
                     TSIDATKAEVARRTIAEKENSFIFVGDPKVNHKGVANAEGIQVISSPKKWKEMTSEEIV
                     EQLRTSRAKITIIPGFKGSSLKLMVAPEQYEELNRRYGEYDARSIMKVVQENGWFSSIE
                     QVYDLKGVGTDNSDSFIIMDTKPSTCEILLPEDIVRLEVEWSFPNWKVPFVERCGGALI
                     RTPYAIVRVDGI"
     gene            5900..6286
                     /locus_tag="KKP64_RS07335"
                     /old_locus_tag="KKP64_07340"
     CDS             5900..6286
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010711281.1"
                     /locus_tag="KKP64_RS07335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07340"
                     /product="hypothetical protein"
                     /protein_id="WP_104669531.1"
                     /transl_table=11
                     /translation="MLVHNKGSYIRHIGNIRLIPGVNDLNSSDAEAFLKGMEIPLNKSL
                     EKLGEIEILDRTTKGKSKETVGFTELSANKAVEAVSDTFDLDLLEKWLEEEQTNKNRTT
                     VVKAIENQIDDIKNPDEDSVVTPD"
     gene            6297..6638
                     /locus_tag="KKP64_RS07340"
                     /old_locus_tag="KKP64_07345"
     CDS             6297..6638
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377536.1"
                     /locus_tag="KKP64_RS07340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07345"
                     /product="DUF4054 domain-containing protein"
                     /protein_id="WP_104669530.1"
                     /transl_table=11
                     /translation="MTKSTVDNVRLTASELSGVSDDAIELFIDDAWIEVNNIFFKDDIK
                     EKACRYLACHLAVLNNQNTKSEQVGSLKKEYSGFHSSFTDLKRTVYGQEYLRLYNKYAK
                     KDLLNMVVL"
     gene            6638..7240
                     /locus_tag="KKP64_RS07345"
                     /old_locus_tag="KKP64_07350"
     CDS             6638..7240
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774206.1"
                     /locus_tag="KKP64_RS07345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07350"
                     /product="hypothetical protein"
                     /protein_id="WP_216788166.1"
                     /transl_table=11
                     /translation="MKITENNRIIKLIEELNQLNKYSLQIGIFGEDDSFIAMLAQVHEF
                     GVTIRPKGRFLVIPLMKKYKGKNPRQFDLFFMQTKENHKFLVRNKGKDQLEFAYMLAEQ
                     VTIPERSFIRSTFDEQSKAWSDYALTLVKKLIDGKMTATELMNRLGLRMQRDIQRTIRN
                     LSDPPNSPITINNKKSSNPLIDTGKLRQSVTYKVVKG"
     gene            7240..7599
                     /locus_tag="KKP64_RS07350"
                     /old_locus_tag="KKP64_07355"
     CDS             7240..7599
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400009.1"
                     /locus_tag="KKP64_RS07350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07355"
                     /product="hypothetical protein"
                     /protein_id="WP_002400009.1"
                     /transl_table=11
                     /translation="MQRMNFSSLIETFAVDFQLVLPSVEGAGKYIHGEWFPTNEEPKTV
                     SGAIIPYDNRTIYQSGGTLTSSDRQLAYVGSIPLGSKIIDMGKEYKVESEEPYAEHYAD
                     VNLYRLKAVTNNAAN"
     gene            7586..8062
                     /locus_tag="KKP64_RS07355"
                     /old_locus_tag="KKP64_07360"
     CDS             7586..8062
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400421.1"
                     /locus_tag="KKP64_RS07355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07360"
                     /product="LIC_12616 family protein"
                     /protein_id="WP_010717320.1"
                     /transl_table=11
                     /translation="MPQINGAFSYELLADELIAIVKKSTGLQLIESSTAGPQPDKPFFS
                     YEVISPYIPVTIDVTDNEVFELVVSIKCHTDSSIQALNLSEQLRKHLNGFSVKVDLQNS
                     KIALVSTTQSQKRDNFISIDYERLAGFDARFRVQDSYVDNAVIIENIDIQEENK"
     gene            8059..9096
                     /locus_tag="KKP64_RS07360"
                     /old_locus_tag="KKP64_07365"
     CDS             8059..9096
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717321.1"
                     /locus_tag="KKP64_RS07360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07365"
                     /product="DUF3383 family protein"
                     /protein_id="WP_010717321.1"
                     /transl_table=11
                     /translation="MIEKITDVNVKIDIMHPQPIVGLGNPAIFVQGSTQNYKEYTSLET
                     LAKDFATTTTVYKKAEIIWKQENKPQTIAVVTFVADTPTSPEQPDSLIAGSGIIAAATS
                     YFYNDWHFALLANFVEADALALSNLIEENEFKFLVIQTATVDELTVFTGNNLTIGLVHP
                     LEEFLDAALIGNTASLTVGSVTWKFRHNLVGITPNTLTTSQLQAIEKANAIAYVSKAGI
                     PQTSEGKTMSGEFIDALHGDHWVKSNIETNVQRLLSTTDKLTFDSNGIALLDTTVANVL
                     ETAFNNGIVDIVDETGVGNYSVTALGRQDLNPDDIAARNYKGLSFKYKRSGAIHTVDVT
                     GTIEV"
     gene            9112..9510
                     /locus_tag="KKP64_RS07365"
                     /old_locus_tag="KKP64_07370"
     CDS             9112..9510
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010711287.1"
                     /locus_tag="KKP64_RS07365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07370"
                     /product="phage protein"
                     /protein_id="WP_002377526.1"
                     /transl_table=11
                     /translation="MQSMTTYDAKEVSTIIDNVVQFGFQDGDMVSFSKDNSYIEVQTDA
                     QGQSSAAKNNDNLGTFTINLSQNSPCNKQLMALANGRKEFAISVTHSTEKAWASKAYIE
                     KTPDGSFGKGVPTRSYTIKALDYKHEYN"
     gene            9563..9895
                     /locus_tag="KKP64_RS07370"
                     /old_locus_tag="KKP64_07375"
     CDS             9563..9895
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377523.1"
                     /locus_tag="KKP64_RS07370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07375"
                     /product="hypothetical protein"
                     /protein_id="WP_002386763.1"
                     /transl_table=11
                     /translation="MTKKDEVKELEVKNNIPEAEKKPFNKFGKQEKHTVEDVEYTFQFP
                     GTRAAQAILDNSKGPSNTFSDVAYHSQLMDSVIVTPKLNWDYWDEHEGYREVMALADNF
                     LGRMLN"
     gene            9936..10076
                     /locus_tag="KKP64_RS07375"
                     /old_locus_tag="KKP64_07380"
     CDS             9936..10076
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386764.1"
                     /locus_tag="KKP64_RS07375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKP64_07380"
                     /product="hypothetical protein"
                     /protein_id="WP_002386764.1"
                     /transl_table=11
                     /translation="MFRWLPVIAGIATKDEVEIATAEELAVWNEVAYQKINLTKSRGGV
                     I"
ORIGIN
        1 gtgctacagg tcgtggagca agactattga ttatagatga tccaattaaa aacagggcag
       61 aagcagagtc taaaacaatt agagataaaa tatacaatga atggcaggat actttttatt
      121 cacgtttaac agcagatgct agcgtaattg taattatgac tcgttggcac gaagatgact
      181 tggcaggacg tttattaaaa gaacaagcat taccgtggga agaaataaaa atacctgcaa
      241 ttgcagaaga gaatgatttg ctaggaagag aaccaggaga agctttagct cctgaaattg
      301 gcaaagatga agaatgggca gctaaaacaa aggctgttac tggctctcgt ggatgggctg
      361 ctttatacca acaaagacca actcctgcag gaggaaatat atttaaacgt tcttggatta
      421 agttttatgt accaacatta gaaaagaaaa tagaatttaa cttaggagat gacgtggtta
      481 ttttaccacg tctttttgat cgtcaagcac agtcttggga ttgcacattt aaagatactg
      541 agacatctga ctttgtttct ggtcaagtct ggggcaaaaa gagagctgat ttttatttgt
      601 tggatcggca tcatgaaaga atggggattg tggaaacaat gaaagctatc aaggttatgt
      661 gtaataagtg gcctaaagct agagggattt acattgagga taaagcaaac ggaaccgctg
      721 ttattgagat gcttaagaaa aagatatctg gaattgttcc cgttactcct gatggtggta
      781 aggaagttag ggctaacgct gttgctcctc tttgggaagc aggaaatgtt tatcttccac
      841 atccgttaat ttgtccgtgg ataaatgatt ttattgatga attagttgca tttcctaatg
      901 ctgaacatga tgacgatgtt gatagtatga ctcaaatgtt aaataaaatg attggtaaag
      961 caagtctaag agaaaggtat cttgaaaact aaaaataaaa ggtggtgagt aaatgggcaa
     1021 tatagcaaat gaagcaaaat tattgaaagt agatggcaaa gcgtacagaa atgatttcat
     1081 gattggtaat ggtaaaggtt acgctaaaga caatttatct agacaaagac caggaataag
     1141 caaaagatta tcatactctc aattagagga tttgtattcg tctaattcaa tggcgaaaaa
     1201 cattgtagat attccagcgg aagatttaac tagaaatggt tggaatttaa aaatgaaaga
     1261 tgaacaggca aaagctttgt acgaaagtaa attaagacag ctaaaaacaa aggatcgttt
     1321 gcaacaatta tttacctacg aacgattata tggtgatggc tttgtcagta ttggaactat
     1381 tgaaaaaagt aattactcat tggataaacc attgaatttt aaaaacatta aaagcgtgcc
     1441 ctatattaat gcattttcag gaaagaaaat aagtaaccga attattaatg aagatgtatt
     1501 tagtcctaat tatggtcaga tagaatcttt tgagattaat aatagatcaa gagatgctcg
     1561 cattgaatta ttacctaatt caacgtatag cactacgaca aagattcatt attcacgagt
     1621 tttacatcag caaaatcttc gttttgaaga tgagcttgca ggttcgtctt tgcttgataa
     1681 tctctatgat attttaacgg tagtagatac ttctatttgg tctgtagggc aaattctata
     1741 tgattatata ttcaaagtat ataaatcttc tgatgtaagt aatcttacat cagaagaaaa
     1801 gttagaaatt gcaatgaaaa tggattataa gtttagaaca gaagctctga ctattattga
     1861 ttcagaagaa agtttaggaa aagaaagttc ttcagtatct ggtattggag agcttttaga
     1921 ttttgtttgg gattacttag caggagctgc tcgaatgcct aagactgttt taaaaggtca
     1981 agaaggtgga actgttactg gagctcaata cgatgttatg aactattatt ctcgtataac
     2041 tgctatacaa gagaaccagt taagaccgca tctcgaatat ctaatccgtt gtttaatgtg
     2101 ggcagaagat gagtgtaatg gtcgtcttga tccagattca atagagtggt ccattgaatt
     2161 caatccttta tggaatgtag atagtaagac tgatgctgaa attagaaaac ttattgcaga
     2221 agctgacaag atatacattg aatcaggtgt agtagatcca gatgaaatcg aagaagcaag
     2281 atttggacga tttggcgtaa ctgaaacatc aaaatttaat gcagatagtt tgtctaggga
     2341 tgagctagat aagatggctg ctatagttta tgaaaattac aaacaggaca gagataatga
     2401 aaaataatcc gaaaacccgt tatcctctgc gtttagaaga aagctacgcc aaaaatatcc
     2461 agaaacttat aaatgaactt gaaaaagtct tgttatatga atttgataaa catctagctt
     2521 ctaaaataga tgaatcaaga ctagtcaatg attcaagatt tattcaagat ggattattcg
     2581 atacagcatc taaactggta aaaaaagtac aaacctattt tttaggcatt tttcaaaata
     2641 gaacagcaca aaaaattgtt cgcaagcata taagcagtgt taatacattt aataaatcta
     2701 atgtaaatgc tcaattaaaa tccagaggaa taaatccttt agaaaatgaa acttggttaa
     2761 ataactatat gaaagctaaa atatcggaaa atgttagcta tattaccaat attcgtgatg
     2821 attactcgca aaaatttgaa cagattattt atagaggagt cactagtgct aaatcatcta
     2881 gtgaaattag agatgaacta gttaaattaa ctgaaatgtc attgaaaaga gcctctttta
     2941 ttgcacgtga tcaaactgga acaattcttg ggaaacttaa tagtgaacgg caaaaaagag
     3001 caggatttag tgcttttcga tggagcgata gcggagatga aagagttcga gattcacatc
     3061 gagagcgaaa tggaaaaatc tatttttatg caaataatcc attattacca ggtgaagaat
     3121 acaattgccg atgtgttgct gaacctgttg atgaggatag cataatagaa aaatcataat
     3181 taaaggaggt gatatagatg gttaaagaaa agaaagctat tgataaggaa acaaaagagg
     3241 taaaagaagt acttaagcct aatagcaaaa cgacacatac agtttctaaa gatgaaacag
     3301 caagtgaaat agcgactaag taccatatga gtctaaaaaa attacttgtt attaataatt
     3361 tagattctgt aaaagaaatc aaggaaggtg ttagtttaat tgtcgagtag gagttgacaa
     3421 aatggtaatt agatatgata aagccttcat tcaggatttt aaagaaactg atgaaggtta
     3481 tttgacgatc actgcgtgcc cgattactcg tccaggagtt tttccttatc gtcgaactga
     3541 cggaggagtg tcaatggaag caaagttacc tgatgaatta ttttcaaaaa caacggtact
     3601 ttcagctaat gctaagccaa tgactgacga tcatccaaca gaaccagtaa cagcagctaa
     3661 ttataataaa tattcaaaag gcatgactca taatgatgcg catgttttag ataataaatt
     3721 agtggtctcg tttactatta cagatgttga gacaatcaaa aaaattaacg atgggaaacg
     3781 tgaattgagt atcggttttc aagcagatgt ttccaaagaa acaggtattt ataatggcat
     3841 gcaatatgat tctgtgcaaa ggaatatgct aattaatcat attgcaatag ttgatgaagg
     3901 cagagctggt ccagaagttg ctatacgtgg tgattcagta gctttcatga ttgattcaaa
     3961 agataaacaa acaggaggaa ataacatgtc aaaattaatt attgattcaa aagaatttga
     4021 agtagaccca attgtaaaag caaaatttga atctttagaa gcaaaattag atgcagcaaa
     4081 acagcgtaac accaatattg aaaaattaga aggtgagcgt gatgctttaa aagctcaaat
     4141 tgatgagttg aataaagaaa ttgctgaagc aaagaaaaaa gaggtaactg gtgatgcgtt
     4201 agataaactt gttcaagacc gtgttgattt aattagtaaa gcacagaagt ttcttggtga
     4261 ttcagctgat ttttctggta aatctgatcg agaaattaaa gaatctgcaa ttgttaaaat
     4321 atcacctgat tttaaaggtg atggaaaatc agatgattat attgcagcgc attttgattc
     4381 agctgtagca catattgaga aaaaaggatt tacaaatcct gctgcattca atgatgcaaa
     4441 aaatcaagat aaagacaaag aaaaagaaga agagattgaa aaacaaaaaa aagctcgatt
     4501 aaacatgaat aaaaaggagg ataaataatt atgactattc cttatccaga aaaatatatg
     4561 aaacctgaat taggtgttgg caaattagca gactatcaag atgtgcaagc tgatagtctt
     4621 gttgttggag cagaaggttt agggtttggt gttggtgtac aagtaaaaga aaatgttgca
     4681 acagcatata aagatggaaa attttatggt gtttcttatg ctaaaaacta tgtagaagaa
     4741 atcccttatg gtgacgttga aaaaattggt aagtacaaag aacatgaaat ggtaccaatt
     4801 ttacgaaaag gttctatttg ggtgaaagta aatgaagatg ttttagcagg tgaagatgcc
     4861 aaagtattaa atggtgggaa ttttggtaaa gctactacat cagatactgt aattggtact
     4921 tttaaaacaa cagcatcgtc tggtaattta gcaattttac aaattaattt accttaatga
     4981 aagtttagga ggaaaaatat atgtcaaatg aagtaacagc aactttagaa gcacgtgact
     5041 tacaagcgat tgataatgtt atttatcaag ccccacaaga ggagcttgta gcacgtacat
     5101 tatttaatgt gaaaacagat attaatccag gagcggaaac atacgcatat aatgttatga
     5161 ctaggagtgg cgctgcaaaa attattgcaa atggtgcaga tgatttgcct ttagttgaca
     5221 ttgatatgaa acgttatcaa tcaccaattt ttacaattgc tgctggtatt cgttatagtc
     5281 gacaagaaat tcgtcaagct caaatgatgg gaacttccat tgatgcaaca aaagcagaag
     5341 tagcccgtcg tactattgct gaaaaagaaa atagctttat ttttgtggga gatcctaaag
     5401 taaaccataa aggtgttgcg aatgctgaag gtattcaagt tattagttca cctaagaaat
     5461 ggaaagaaat gactagtgaa gagattgttg aacaattacg tacatctcga gctaaaatta
     5521 ctattattcc aggatttaaa ggatctagtc taaaattaat ggttgctcca gaacaatatg
     5581 aagaattgaa tcgtcgttat ggtgaatatg atgcacgatc aattatgaaa gttgttcagg
     5641 agaatggctg gttttcatct attgagcaag tttatgattt aaaaggtgta ggtactgata
     5701 attctgattc atttattatc atggatacaa aaccatcaac ttgtgaaatt ttacttccag
     5761 aagacatcgt gcgtttagaa gttgagtggt ctttcccaaa ttggaaagta ccatttgtag
     5821 agcgttgtgg tggtgcgtta attagaacgc catatgcaat cgttcgtgta gatggtattt
     5881 aaaaaaggag gtaaagatta tgttagtaca caataaaggt tcatatatta gacatatcgg
     5941 aaatattcga ttaattccag gagtaaatga tttaaatagt tcagatgctg aagcatttct
     6001 taaaggtatg gaaataccat taaataaatc attggaaaaa ttaggagaaa ttgagatttt
     6061 agaccgtaca acaaaaggga aatcaaaaga aacagttggt tttactgaat tgagtgccaa
     6121 taaagcagta gaagctgttt ctgatacttt tgatttagac ttgttggaaa aatggttaga
     6181 ggaagagcaa actaataaaa atcgaacaac agtagtaaaa gctattgaaa atcaaattga
     6241 cgacatcaag aatccagatg aagatagtgt agtcacacca gattaggagc gatcttatga
     6301 ccaaaagtac agttgataat gtccgattaa cagctagtga attatcagga gttagtgatg
     6361 atgctattga gttatttatt gatgatgctt ggattgaagt aaataatatt ttttttaaag
     6421 atgacataaa agaaaaagct tgtcgttatt tagcttgtca tttggctgtt ttgaacaatc
     6481 aaaatacgaa atctgagcaa gtaggttcgt taaaaaaaga atattcaggt tttcattcgt
     6541 cttttaccga tttaaaaaga acagtctacg gacaagaata tcttcgtctt tataataaat
     6601 acgctaaaaa agatttatta aatatggtag tactctaatg aaaattaccg aaaataatag
     6661 gattattaag ctgattgaag agctgaatca gcttaataag tattcattac agataggaat
     6721 attcggagaa gatgactctt ttatagctat gttggctcaa gttcatgaat ttggagttac
     6781 tatccgtcca aaaggtagat ttcttgttat accattgatg aagaaataca aagggaagaa
     6841 tccacgtcaa tttgacttat tttttatgca aacaaaagaa aatcataaat ttttggtaag
     6901 aaataagggg aaagatcaac tagaatttgc ttatatgctg gcagagcaag taacaattcc
     6961 tgaacgatca tttattaggt ctacatttga tgaacaatct aaagcgtggt cagattatgc
     7021 actaactctt gtaaaaaaat taattgacgg taaaatgact gctacagaat taatgaatcg
     7081 attaggatta cgaatgcaga gagatattca acgaacaatc agaaatttat ctgatccacc
     7141 aaattctcca attacaataa ataacaaaaa atcaagtaat ccattaatag atacaggaaa
     7201 actaaggcaa tcagtgactt ataaggtggt gaaagggtaa tgcaaagaat gaacttttct
     7261 agtcttatag aaacttttgc ggttgacttt cagttagtgt taccttcagt tgaaggtgct
     7321 ggtaaataca ttcacggcga atggtttcct actaacgaag aacccaaaac cgtttctggg
     7381 gcgattatcc catacgacaa tcggacgatt tatcaaagtg gtggaacact cacatctagc
     7441 gacaggcaac ttgcttacgt tggatcgatt cctttaggtt ctaaaattat tgacatggga
     7501 aaagagtaca aagtagaaag cgaagagcca tatgcggaac actatgcaga tgtgaacctt
     7561 tacagattaa aggcggtgac caataatgcc gcaaattaat ggggctttta gttatgagct
     7621 tttagctgat gagctaatag caattgttaa gaaatcaact ggacttcaac ttattgaaag
     7681 tagcacagct ggtccacagc cagataaacc gtttttttct tatgaggtca tctcgccata
     7741 tattccagtg actatagatg tcactgataa tgaagtattt gaattagtag tgtctattaa
     7801 atgtcataca gattctagta ttcaggcact taatttgtca gagcagttaa gaaagcattt
     7861 aaacggtttt tctgtaaaag tagaccttca aaactctaaa atagccttag tttcaacaac
     7921 acaatctcaa aaacgagata atttcattag tattgattat gaacgtctgg ctggctttga
     7981 tgctcgtttt agagtacaag acagctacgt tgacaatgct gtgattatag aaaatataga
     8041 catacaggag gaaaacaaat gattgaaaaa attacagatg ttaatgtaaa aattgacata
     8101 atgcatccac aacccattgt tggattagga aatccagcaa tttttgttca aggatctact
     8161 caaaactata aagaatatac aagtttagaa acattagcaa aagattttgc tacaacaact
     8221 actgtttata aaaaagcaga aataatttgg aaacaggaaa ataaaccaca aactattgca
     8281 gtagtaactt ttgtagcaga tacccctact agtccagaac agccagatag cttaattgct
     8341 ggaagtggaa ttatcgccgc agctacaagt tacttttata atgattggca ttttgcatta
     8401 ttagccaact ttgttgaggc agatgctttg gcactatcaa atttaattga agaaaacgaa
     8461 tttaagtttt tagtaattca gacagctaca gttgacgaat taacagtttt tacagggaac
     8521 aatctaacta tcggcttggt tcatccgtta gaagaatttt tagatgctgc attaattggt
     8581 aatactgcaa gcttaacagt cggaagtgtt acttggaaat tccgacataa tttagtaggg
     8641 attacaccta acacattaac tacctctcaa ctacaagcga ttgagaaagc taatgctatt
     8701 gcttatgtat caaaagcggg aatcccccaa acatcagaag gaaaaacaat gagtggcgaa
     8761 tttatcgatg ccctgcatgg ggatcactgg gttaaatcaa acattgaaac gaatgttcag
     8821 cgcttgttat caacgacaga taaattaact tttgattcta atgggattgc tttattggat
     8881 acaactgttg caaatgtttt ggaaactgca tttaataacg gaattgtaga tattgtagat
     8941 gaaactggtg ttggaaatta tagtgtgact gctttgggac gtcaagattt aaatccagat
     9001 gatattgcag cacgaaacta taaaggatta tcatttaaat acaaacgttc tggagcaatt
     9061 cacactgttg atgttactgg aacaattgaa gtctaaaggg ggaactaact aatgcaaagc
     9121 atgacaactt atgatgccaa agaagtatct actattattg acaatgtcgt ccaattcggc
     9181 tttcaagatg gcgatatggt atctttttca aaagataatt cgtatattga agtacaaaca
     9241 gatgcccaag gacaatctag tgctgcgaag aacaacgata atttagggac ttttacaatt
     9301 aacttgtctc aaaattcacc atgtaacaaa cagctgatgg ctttagctaa tggtcgtaaa
     9361 gaatttgcaa tttctgtgac acattcaact gaaaaagcat gggcatcaaa agcctatatt
     9421 gaaaaaacac ctgatggatc gtttggtaaa ggcgttccga ctcgttctta tacgattaaa
     9481 gcattagatt acaaacacga atataactaa gcgcttaaca tctcgttaag tgctttttat
     9541 ttaactttag gaggaattta tcatgacgaa aaaagatgaa gtaaaagaat tagaagtgaa
     9601 aaataacatt ccagaagcag aaaaaaaacc atttaataag ttcggaaaac aagaaaaaca
     9661 tactgttgaa gatgtggaat atacatttca attccctgga acacgtgcag ctcaagcgat
     9721 tttagataac tcaaaaggac cgtcaaatac tttttctgac gttgcttacc actctcaact
     9781 tatggactca gttattgtta caccaaaatt gaactgggac tattgggatg aacacgaagg
     9841 atatcgtgag gttatggcat tagccgacaa ctttcttggt cgaatgctta actagcccta
     9901 atccgagaat tacggaaaga gaagttcaaa aagatatgtt taggtggctg cctgtaatag
     9961 caggcattgc cactaaagat gaagtcgaaa ttgccacggc agaggagcta gcagtttgga
    10021 acgaagtagc atatcaaaaa ataaatctaa ctaaatcaag aggaggtgtc atctaataat
    10081 ggcagatgcg ttacgtagtt cagtaatcga actcgattgg aaaataaata atagatcgtt
    10141 agagcgtgcc aatgaagaaa ctgataaaat tcttgctaaa gctgcacgaa tggaaggtac
    10201 ttatcaaaat tcagcaaaat ccatagatgg cgccacaacc tctttaaaaa gaaatagtga
    10261 aggtttaaaa caaaatacag ataaagttgt ccagttcgga aatcgagcaa aagattctat
    10321 gcaaaagacg acaagctctg ctaaacaaac tgaaaaacaa gtaaaagatg ttggaagtca
    10381 gtttgataaa agtaaaaatt ctgcaagtgt tttcgctcaa tctagtgcaa catctctaaa
    10441 agtagttgga aaagctgcga agggtgtaca aacaagtatt ggccatatag gtactgttgc
    10501 aacaaaggct tcagatgttg cttggaatgc ttttacaaag ataagaaatg gtgcaatgat
    10561 agctggtgca gcaatcgcag gagcaggcaa aaaggccttt gactatgcat ctgatactaa
    10621 tgaagcttta aacaaagtag aagtagcttt tggtgataat aataaagttg tggaagattg
    10681 gtcgaagtcc acactgacta atattggttt ggcacaaggt acagcgttag acttagcagc
    10741 tacatatgga gatatgtcaa cttcaatggg tattggtaca gaagaagctg caaaaatgtc
    10801 tacttcatta gttgacttgg caggagatct tgcttcattt aaaaatatag gaattgaccg
    10861 tgtaaatact gcattaaatg gt
//
