LOCUS       NZ_CP076538            10417 bp    DNA     circular CON 16-DEC-2024
DEFINITION  Amycolatopsis aidingensis strain YIM 96748 chromosome, complete
            genome.
ACCESSION   NZ_CP076538
VERSION     NZ_CP076538.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis aidingensis
  ORGANISM  Amycolatopsis aidingensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 7657695)
  AUTHORS   Li,R.
  TITLE     YIM 96748 draft genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7657695)
  AUTHORS   Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2021) Yunnan University, Yunnan Institute of
            Microbiology, 121 Street, Yunnan 650000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdevono v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018885265.1-RS_2024_12_16
            Annotation Date                   :: 12/16/2024 14:59:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,118
            CDSs (total)                      :: 7,060
            Genes (coding)                    :: 6,941
            CDSs (with protein)               :: 6,941
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 44 of 119
            Pseudo Genes (incomplete)         :: 87 of 119
            Pseudo Genes (internal stop)      :: 2 of 119
            Pseudo Genes (multiple problems)  :: 13 of 119
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 14:56:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6614486
            Orig. end                         :: 6624903
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076538.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="31"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5417](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5417)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(502..517)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 22.03"
                     /tool="antismash"
     misc_feature    530..545
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.77"
                     /tool="antismash"
     gene            complement(845..3703)
                     /locus_tag="KOI47_RS30205"
     CDS             complement(845..3703)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669888.1"
                     /locus_tag="KOI47_RS30205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_216210181.1"
                     /transl_table=11
                     /translation="MGSRTRERDVDARDTGAGESTEPAEVRIQLLGPVQLIADGRQVPV
                     GGPGVRGLLALLALDANKVVTLDDIIDGLWGHEPPATARTIVHGNVSHLRRVLRSLHGE
                     HPDPAGAAPVEIHTATPGYRLTIAPDRVDVHRARALLERAAVAPVERRAELLGEAFALW
                     QGPVLGGVPASIAAPELEELRQAVHGARVDADLELGKHAELIAELAPLVRENPADERAV
                     RQLMLALYRSGRRADALEVYRQASRQVGERLGIDLGPELRTLHDQLLKDELAEHTGWPR
                     TRLALTQLPPAVPSLAGRAPELSWLNDLRVRAQQGARAIGVLTGAAGIGKSALAISWAH
                     EAVDAFEDGVLFAALRGFDPHREPADPAEVLSHFLLGLGVPAAELPERLPERIALYRSL
                     LAEKKVLVLLDDARTAEQVRPLLPPGARSLTLITSRSRLDGLAVSNAAKLYALDVLTAQ
                     DAVRLIEELAGPDPQGRHARLAELCGYLPLALRIAGARLAAAPEWTVTQLLEELASERT
                     RLAALDLADADGGDTSVRAALDVSYRGLPAAAAAIFRALGALTSCSAGVGLAATACGVD
                     PETARRGLRVLAAHHLLTETAPDVFTPHDLVRLYLRDLAAAELTGAERTAVLTRALHYY
                     QASADRARRRLLRIVDPLEFTGLAGIAADPTGYDEALDWFVAEWPNLLAIIRQARSRGL
                     HTEAWQLARVVHTYRVVRPLWDEWRLLVDLGLASAEDSGSLLGRCWMLISRCAVALIFG
                     RAESSLADAEQALELAGELGDERLLISANIHLGCALTLCERYQEAISRLSAAIEDTERL
                     GDLALGGQALNNCAEAEKRAGRYHEAIAHQLRSLEIDRKLGDDSYAVVSLNNLAEMQLA
                     IGRPAESQRYAREAIAVTEARGLLLQEGVARLTLGRVLRTMGDFEGARAQFAASLELHE
                     RVSPHAADEVRAELEAVPPADS"
     misc_feature    complement(989..991)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(4022..4921)
                     /gene="thpD"
                     /locus_tag="KOI47_RS30210"
     CDS             complement(4022..4921)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230318.1"
                     /locus_tag="KOI47_RS30210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_408629864.1"
                     /sec_met_domain="PhyH (E-value: 3.9e-35, bitscore: 120.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLTDTRVEDSYPTRIAGQPELMDRVDPTVWGSESDGPIDAADLAS
                     HDGKGYSVVEGLLSPAEVQGYWQELVRLSSDQELKADERVVTEKASGEVRSIFEVHKIS
                     ELISELARDPRVLDRARQILGSEVYIHQSRVNYMPGFRGSGFYWHSDFETWHAEDGMPR
                     PRAVSCSIALTDNYPFNGGLMVMPGSQRTFVQCIGQTPEDYYKTSLKEQRIGVPTEDDI
                     TKMAAEHGIDQFTGPAGSALFFDSNIMHGSGNNITPYPRSNIFLVFNSVENTLVEPFAA
                     SKRRPGFIGSRDFTPLSR"
     gene            complement(5001..5417)
                     /locus_tag="KOI47_RS30215"
     CDS             complement(5001..5417)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947387.1"
                     /locus_tag="KOI47_RS30215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_216210185.1"
                     /sec_met_domain="ectoine_synt (E-value: 5.1e-55, bitscore:
                     183.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTLDEITDTDADIKTPNWRSKRIVLAKEKAGFSVHETTLYAG
                     TVNDFWYANHIEAVFVFEGEGEITDKATGETHQLKPGSLYLLNNHDKHQVRPRTDMRTV
                     CVFNPPVTGREVHDENGVYPIVREEDDLADGVTS"
     gene            complement(5463..6716)
                     /gene="ectB"
                     /locus_tag="KOI47_RS30220"
     CDS             complement(5463..6716)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 2.6e-72.
                     Score: 235.6. Matches aSDomain:
                     nrpspksdomains_KOI47_RS30220_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787752.1"
                     /locus_tag="KOI47_RS30220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_216210187.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.2e-74, bitscore:
                     250.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFETLESEVRSYSRSWPTVFDRAQGSWLYAEDGKAYLDFFAGA
                     GALNYGHNNPALKKALIDYISRDGVTHSLDMFTVAKRDFLESLDEVILKPRSMEYKVIF
                     PGPGGANAVEAALKLARKVTGKESVINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHA
                     TPMPYDKYFDGAYPDFLYFERLLEDSGSGLNEPAAVIVETVQGEGGINAARLEWLQGLS
                     ELCKRHGILLILDDVQMGCGRTGPFFSFEDAGIKPDMICLSKSIGGYGLPMALTLINPE
                     LDVWSPGEHNGTFRGISPAFVTATEALRTYWRDDELEKSTKAKGERIGGVFNEIVRTYP
                     DAQLLAKGRGLARGLEFPDGETAGKVCAAAFDRGLLMETSGPDGEVVKLLPALTTTDSE
                     LTQGLEIIEESVKAVLAP"
     aSDomain        complement(5643..6653)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KOI47_RS30220_Aminotran_3.1"
                     /evalue="2.60E-72"
                     /label="KOI47_RS30220_Aminotran_3.1"
                     /locus_tag="KOI47_RS30220"
                     /protein_end="358"
                     /protein_start="21"
                     /score="235.6"
                     /tool="antismash"
                     /translation="FDRAQGSWLYAEDGKAYLDFFAGAGALNYGHNNPALKKALIDYIS
                     RDGVTHSLDMFTVAKRDFLESLDEVILKPRSMEYKVIFPGPGGANAVEAALKLARKVTG
                     KESVINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHATPMPYDKYFDGAYPDFLYFER
                     LLEDSGSGLNEPAAVIVETVQGEGGINAARLEWLQGLSELCKRHGILLILDDVQMGCGR
                     TGPFFSFEDAGIKPDMICLSKSIGGYGLPMALTLINPELDVWSPGEHNGTFRGISPAFV
                     TATEALRTYWRDDELEKSTKAKGERIGGVFNEIVRTYPDAQLLAKGRGLARGLEFP"
     misc_feature    6760..6775
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.19"
                     /tool="antismash"
     gene            complement(6790..7323)
                     /gene="ectA"
                     /locus_tag="KOI47_RS30225"
     CDS             complement(6790..7323)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787753.1"
                     /locus_tag="KOI47_RS30225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_216210188.1"
                     /transl_table=11
                     /translation="MSGKHIQRANGDNAAADHVVIESPAKSDGAALWRIARDSQKLDLN
                     SSYAYLLWCHDFADTSVVARVDGVAVGFVIGYRRQSKQDAAVVWQVAVDDSQRGRGLAG
                     RMLDTLFEGLVRRGVRYLETTITPDNDASIRLFRSFAKRWDAELETAELFAAADFPDEH
                     EAEHIYRIGPLAGA"
     gene            7636..8601
                     /locus_tag="KOI47_RS30230"
     CDS             7636..8601
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KOI47_RS30230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_216210190.1"
                     /transl_table=11
                     /translation="MYSTTVRSGDRQHCRTGKPARTVETSLILLYLLVFRFALWVGFSP
                     LRLAGRIYGRYGFAREFQFRAGDVGLPDEPANTVLERLLTARALIALGVLTIAVQAVLD
                     PPSALRKLASIGSWQDGLVVLLTAPVGISLLAPLLVVLARGGHRARTARALVRPVATGL
                     FSALLLVIVAAPPIPVVTGTLDRIVGWLSQGVLRVPLVAMAGDERVPPVLLFPLLWVLL
                     FFVGAAYLAQRNGLCARKDHDLLWLLAPLLSVWSSWWIALTKLGAPPEQLAALEPWEYT
                     LSWLATPVTVTVLAAVEIRKLRADGIRFRTRNPARLRLAR"
     gene            8610..9095
                     /locus_tag="KOI47_RS30235"
     CDS             8610..9095
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024721.1"
                     /locus_tag="KOI47_RS30235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_216210192.1"
                     /transl_table=11
                     /translation="MREVTIRPAKRDELAAVAELRWQWGLERAMEHGDSPGTGHEEFVA
                     RFLDWAGRHGHSHHCLVAVRDGLVIGMAWLAVLQRVPTPLAVERASGDLQCVYVVPGER
                     DGGLGGLLIQAVLALARELGLERVTVHSSIRAVPAYARHGFAASPCLLQAEPATSSR"
     gene            9130..9705
                     /locus_tag="KOI47_RS30240"
     CDS             9130..9705
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222739.1"
                     /locus_tag="KOI47_RS30240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="WP_216210194.1"
                     /transl_table=11
                     /translation="MPNTLTLTGDAEADKLLSEDPFALLTGMLLDQQIAMEVAFAGPRK
                     IAERMDGFSVTRIAESDLEEFVELCVQKPAIHRYGGSMARRVHTLARHIVDSYGGRTEA
                     IWTEGDPDGTEVLKRLKALPGYGEQKAKIFLALLGKQLGVQPAGWREAAGAYGEDGARR
                     SIADVTGQDTLAEVRAFKKAAKAAAKKG"
     misc_feature    9894..9907
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.99"
                     /tool="antismash"
     misc_feature    complement(10153..10166)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.04"
                     /tool="antismash"
ORIGIN
        1 ccgaccggga gaaggccccg ctgatccagc gcgggcaggc gggcaacacg atcttcttcg
       61 gcttcccgga cacggtggac gaggacgcgc tgatccacga cggcatcgag acgctgtccg
      121 agcgcgcggt caaggaactg tgcgacttcc tcccggtcaa cggttccgac gacggcgccc
      181 tggatgccgt gctgctgcag cgggacaccg tgcgcaacgc ggtgagcgac atcgtgaacg
      241 cggtggccaa gaacgccggg gtcgggctca ccctttcccc taccaccggt gaggaggtgg
      301 cccgcagcat gaccgccgag caggccaggg tgctgcaggg cagcctgcgg gagtccaggg
      361 aggacgtcgg ctacgagacc gtcaccggca ggctggacgg agtgcgtacc cggcggcgga
      421 tcttctacct ggagcgggac agtggcagca cgatccaggg tgcggtggca ccggacctgc
      481 tggacgagat caagctgaac ctggaccagc cggtcaccgc gcggctgcga gtggtccgga
      541 ccaccaccat cgacggcagg cgcggccgcc cggtgtacga gttgctggag atccgctccg
      601 agaccagcct cttcgatcag gaaccgtccg gatagcaacc gcccgtctgg gcggaccggc
      661 cggtgctctc ccctgtgtca ccggccggtc cgcgacggat cgtctcgggt gacccgtcca
      721 cgtccacgga tccatccccc tgcacccgtg gatgtggcgc cccaccggcc cctcccccag
      781 ggccatgtga tcggcgccgc tttcgcacac tacgccgaat gccagccagg ccactaagaa
      841 ctcgctaaga gtccgcgggt ggcaccgcct cgagctcggc ccgtacctcg tccgccgcat
      901 gcggactcac ccgttcgtgt agctccaacg aggcggcgaa ctgggcacga gcgccctcga
      961 aatcacccat agttcgcaaa acccgcccta atgtgagacg cgccacaccc tcttgcagga
     1021 gcagcccgcg cgcctcggtc accgcgatcg cctccctggc gtaccgctgg gactccgcag
     1081 gcctgccgat cgccagctgc atctcggcga ggttgttcag cgacaccacg gcataactgt
     1141 cgtccccgag cttacgatcg atttccagcg aacggagctg gtgtgcgatc gcctcgtggt
     1201 agcggcctgc gcgtttctcc gcctccgcgc agttgttcag cgcctgcccg cccagtgcga
     1261 ggtcgccgag tcgttcggtg tcctcgatcg cggcgctcag ccgggagatg gcctcctggt
     1321 agcgctcgca gagggtcagc gcgcagccga ggtggatgtt cgccgagatc agcagccgct
     1381 cgtcgccgag ttcgcccgcg agctccagcg cctgctcggc atcggcgagg ctgctctcgg
     1441 cccggccgaa gatcagcgcc accgcgcacc gcgagatcag catccagcag cggccgagca
     1501 ggctgcccga gtcctcggcc gaggccagcc cgagatccac cagcagccgc cactcgtccc
     1561 acagcggtcg cacgacccgg taggtgtgca ccaccctggc cagctgccag gcctcggtgt
     1621 gcagcccccg ggagcgggcc tggcggatga tcgccagcag gttcggccat tcggccacga
     1681 accagtccag cgcctcgtcg tacccggtgg gatcggcggc gatcccggcc agcccggtga
     1741 actcgagcgg gtcgacgatg cgcagcaacc gcctgcgcgc ccggtccgcg gaggcctggt
     1801 agtagtgcag cgccctggtg agcaccgcgg tgcgttccgc cccggtcagc tcggccgcgg
     1861 cgaggtcccg caggtacagc cggaccaggt cgtgcggggt gaagacgtcc ggagcggtct
     1921 cggtcagcag gtggtgcgcg gccagcaccc gcagcccgcg ccgcgcggtc tcggggtcga
     1981 caccgcaggc ggttgcggcc aggcccaccc cggccgagca ggaggtgagc gcgccgaggg
     2041 cgcggaagat cgcggcggcc gctgccggca ggccgcggta ggacacgtcc agcgcggccc
     2101 gcacgctggt gtccccgccg tcggcgtccg cgaggtccag cgcggccagc ctggtccgct
     2161 cgctcgccag ttcctcgagc agctgggtca ccgtccactc cggggccgcg gcgagccgcg
     2221 cgcctgcgat ccgcagggcc agcgggaggt agccgcacag ctcggcgagc cgtgcgtgcc
     2281 tgccctgcgg gtcgggcccg gccagctcct cgatcagccg gaccgcgtcc tgcgcggtga
     2341 gcacgtccag ggcgtacagc ttggccgcgt tggacaccgc aagaccgtcc aaccgggacc
     2401 ggctggtgat cagggtcagt gagcgcgcac ccggcggcag cagcgggcgg acctgctcgg
     2461 cggtgcgggc gtcgtccagc agcaccagca ccttcttctc ggccagcagc gagcggtaca
     2521 gcgcgatgcg ttccggcagc cgctcgggca gttcggctgc cggcaccccg aggccgagca
     2581 ggaagtggga gagcacctcc gcggggtcgg caggctcgcg gtgcgggtcg aacccgcgca
     2641 gcgcggcgaa cagcaccccg tcctcgaagg cgtccacggc ctcgtgcgcc caggagatgg
     2701 ccagcgcgct cttgccgatc ccggcggctc cggtgagcac gccgatggca cgcgccccct
     2761 gctgggcccg cacccgcagg tcgttcagcc aggacagctc gggggcgcgc ccggcgaggc
     2821 tgggcacggc cggtggcagc tgggtgagcg cgagccgggt acgcggccag ccggtgtgct
     2881 cggcgagctc gtccttgagc agttggtcgt gcagggtgcg cagctcaggg ccgaggtcga
     2941 tgccgagccg ctcgccgacc tgccgggagg cctgccggta cacctcgagc gcatcggccc
     3001 gccgcccgga gcggtacagc gcgagcatca gctgccgcac cgcgcgctcg tccgccgggt
     3061 tctcccgcac cagcggggcc agctcggcga tcaactcggc atgcttgccg agctcgaggt
     3121 cggcgtccac cctggcgccg tgcacggcct ggcgcagttc ctccagctcg ggagcggcga
     3181 tggaagcggg taccccgccg agcaccggcc cttgccacag cgcgaaggcc tcaccgagca
     3241 gctccgcccg ccgctccacc ggggcgaccg cggcgcgctc cagcagggcg cgggcgcggt
     3301 gcacgtccac ccggtccggg gcgatcgtca gccggtatcc cggggtggcg gtgtggatct
     3361 cgaccggagc ggcgcctgcc gggtccggat gctccccgtg cagcgagcgg agcacccggc
     3421 gcaggtggga cacgttgccg tgcacgatgg tccgcgcggt ggcaggtggt tcgtggcccc
     3481 agagcccgtc gatgatgtcg tccagggtga ccaccttgtt ggcgtccagc gccagcaggg
     3541 ccagcaggcc acgcacccct gggccgccga ccggtacctg cctgccgtcg gcaatcagct
     3601 gtaccgggcc gagcagctgg atccgcacct cggccggctc cgtcgactcc cccgcgccgg
     3661 tgtcgcgtgc gtccacatcg cgctcccgtg tccgactccc cattcgtaaa cggacctcga
     3721 aagtaccgcg cggccaggag agcgcggggc gtttcggccg ggatcgaccc accagccgga
     3781 caagcacacc cgatcagacg agtgcggccc cgcaccatca gtcccgatct gatggtgcgg
     3841 ggccgcactc ggtgctactc gacggcggat cgcaggacgc tccgccggat gtcttcgcct
     3901 ccacccggtc cggatctcac cccgagcgaa aatccggacc accttcagcg tgacggggac
     3961 aacataacag ggcgcgagcg gaccgctaca ggcggcccca ctcgcgccct gttttacctg
     4021 gtcagcgcga cagcggggtg aagtccctgc tgccgatgaa ccccggccgc cgcttgctcg
     4081 ccgcaaacgg ctccaccagc gtgttctcca cgctgttgaa caccaggaag atgttcgagc
     4141 gcgggtacgg cgtgatgttg ttgccggaac cgtgcatgat gttggagtcg aagaacagcg
     4201 ccgagcccgc cggaccggtg aactggtcga tcccgtgctc ggccgccatc ttggtgatgt
     4261 cgtcctcggt cgggacgccg atccgctgct ctttgagcga ggtcttgtag tagtcctccg
     4321 gcgtctgccc gatgcactgc acgaacgtac gctgcgaccc cggcatgacc atcaggccgc
     4381 cgttgaacgg gtagttgtcg gtcagcgcga tcgagcagct caccgcacgg gggcggggca
     4441 taccgtcctc cgcgtgccag gtttcgaagt cggagtgcca gtagaagccg gaaccccgga
     4501 aacccggcat gtagttgacc cggctctgat ggatgtagac ctctgacccg aggatctgcc
     4561 gcgcccggtc gaggaccctc gggtccctgg ccagctcgct gatcagctca ctgatcttgt
     4621 gcacctcgaa gatcgaccgg acctcgccgg aagccttctc cgtgacgaca cgctcgtctg
     4681 ccttcagctc ctggtccgag gacagccgga ccagctcctg ccagtagccc tggacctcgg
     4741 caggcgaaag cagaccctcg acgaccgagt agcccttgcc gtcatgcgac gcaaggtcgg
     4801 cggcgtcgat agggccgtcg gactcgctgc cccacacggt cggatccacc cgatccatga
     4861 gttccggctg acccgcgatc cgggtcgggt agctgtcctc cacacgagtg tccgtgagca
     4921 tcaagctgct cgcctcctca tgattccttc ctgtttttct gccccattct ttggtggtag
     4981 ggacgccagt cagtccggcg tcaggaggtg acgccgtccg caaggtcgtc ttcctcgcgg
     5041 acgatcgggt acacgccgtt ctcgtcgtgc acctccctgc cggtcaccgg cgggttgaag
     5101 acgcacaccg tgcgcatgtc cgtccgcggc cgtacctggt gcttgtcgtg gttgttcagc
     5161 aggtacaggg agccgggctt gagctggtgc gtctcgccgg tggccttgtc ggtgatctcg
     5221 ccttcgccct cgaaaacgaa caccgcctcg atgtggttcg cgtaccagaa atcgttgacg
     5281 gttcccgcgt acagcgtggt ctcatgcacc gagaagccgg ccttctcctt ggccagcaca
     5341 atccgcttgc tgcgccagtt cggagtcttg atgtcggcgt cggtatcggt gatctcatcg
     5401 agggtgcgga cgatcaaaac actctccttt ggttcttccg cgacatgcgt gctctgggtg
     5461 gttcagggcg cgagtaccgc cttgaccgac tcctcgatga tctccaggcc ctgggtgagc
     5521 tcggagtccg tggtggtcag cgcgggcagc agcttcacga cctcaccgtc cgggccggag
     5581 gtctccatca gcaggccccg gtcgaaggcg gctgcgcata ccttgccagc cgtctcgccg
     5641 tccggaaact ccaggccgcg ggcgaggccg cggcccttgg ccagcagctg cgcgtccggg
     5701 taggtccgca cgatctcgtt gaacacgccg ccgatccgct cgcccttggc cttggtggac
     5761 ttctccagct cgtcatcgcg ccagtaggtg cgcagcgcct cggtggcggt gacgaaggcc
     5821 gggctgatgc cacggaaggt gccgttgtgc tcaccgggcg accacacgtc cagctcgggg
     5881 ttgatcaggg tgagcgccat cgggaggccg tagccgccga tggacttgga aaggcagatc
     5941 atgtccggct tgatgcctgc gtcctcgaag ctgaagaacg ggccggtgcg cccgcagccc
     6001 atctgcacgt cgtccaggat cagcaggatg ccgtgccgct tgcacagctc ggagaggccc
     6061 tggagccact ccagcctggc tgcgttgatc ccgccctcgc cctgcacggt ctccacgatc
     6121 accgcggcgg gctcgttcag cccgctgccg gagtcctcca gcaaccgctc gaagtagagg
     6181 aagtccgggt aggcgccgtc gaagtacttg tcgtagggca tcggcgtggc gtgcaccagc
     6241 gggatgcccg cgccgccgcg cttcatcgag ttcccggtca ccgagagggc gccgagggtc
     6301 atgccgtgga aggcgttggt gaagttgatg accgactcct tgccggtcac cttgcgggcc
     6361 agcttcagcg cggcctccac cgcgttggcg ccgccgggtc ccgggaagat caccttgtac
     6421 tccatggaac gcggcttcag gatcacctcg tccaggctct ccaggaagtc ccgcttggcc
     6481 acggtgaaca tgtccaggga gtgggtgaca ccgtcccggg agatgtagtc gatcagcgct
     6541 ttcttcagcg ccgggttgtt gtgcccgtag ttgagcgccc ccgccccggc gaagaagtca
     6601 agataggctt tgccgtcctc ggcgtagagc caactcccct gcgcccggtc gaacacggtc
     6661 ggccagctac gactgtagct gcgcacttcc gattcgagcg tttcgaagat gctcacacta
     6721 actccgtctc ttactctatt cgtgcccagc gggcgactat taacgtattc ttgtcacccg
     6781 ggcgacgtat cacgcaccgg caagcggacc gatgcggtaa atgtgttccg cctcgtgctc
     6841 atccgggaaa tccgcggccg cgaacagctc cgcggtctcc agttcggcgt cccaccgctt
     6901 ggcaaaggag cggaacagcc ggatggacgc gtcgttgtcc ggtgtgatgg tcgtctccag
     6961 gtagcgcacg cctcgccgca ccaacccctc gaacagggta tccagcatgc ggccggccaa
     7021 accacggccg cgttgcgagt cgtccacggc gacctgccac acgacggcgg cgtcctgctt
     7081 cgactggcgg cggtagccga tcacgaatcc gacggccact ccgtccactc gcgccaccac
     7141 ggaggtgtcg gcgaaatcat gacaccacaa caagtaggcg tacgacgagt tcaggtccag
     7201 cttttgtgaa tcgcgcgcga ttcgccagag ggccgcacca tccgacttgg cgggtgattc
     7261 gatcaccaca tggtcggccg ctgcattgtc gccgttggca cgctgtatgt gctttccgga
     7321 catatcaatg caacctaaca gaagcttttg cccagctcag ccgcgctgca cccgcggcag
     7381 gcgacctacc tgcggcgatg aggaaacacc tgtgagaatg acgacacaag tgtaccgtct
     7441 gcgcggtcgc tgtgctagga gcgcagcacg aacggcctcg gcttggccgc ggtacccgca
     7501 cgctgaccag cgccgaggct gactacgcgc acacggtagt gggacgacag cggaaacgcg
     7561 agtgggtaag cgccgaatac tccactcggc cgcagcggca cgcacaccac gggttacgac
     7621 aatccggtga tccgcgtgta ttcgaccacc gtacgtagtg gcgatcggca acactgccgg
     7681 accggcaagc cggcccggac cgtggagacc tcgttgatcc tgctttacct gctcgtgttt
     7741 cgcttcgccc tgtgggtggg gttcagtcca ctgcgcctgg ccggccggat atacggcagg
     7801 tacggattcg ccagggagtt ccagttccgg gccggcgacg tcgggctgcc ggacgagccg
     7861 gcgaacaccg tgctcgagcg gctgctcacc gcgcgcgccc tgatcgcgct cggcgtgctc
     7921 acgatagcgg tgcaggccgt gctcgacccg ccgtcggcgc tgcgcaaact tgcctccatc
     7981 ggtagctggc aggacggcct ggtcgtcctg ctcacggctc cggtcgggat cagcctgctc
     8041 gctccgctgc tggtcgtgct cgcgcgcggc ggccatcgtg ctcgtacggc ccgcgcactc
     8101 gtccggccgg tggcgaccgg cctgttcagc gcgttgttgc tggtcatcgt cgcggcgcca
     8161 ccgatcccgg tggtcaccgg cacgctggac cggatcgtcg gctggctcag ccagggtgtg
     8221 ctccgcgtgc cgctggtcgc gatggcaggc gacgagcggg tcccgccggt gctgctgttc
     8281 ccgttgctct gggtgctgct gttcttcgtc ggcgcggcct accttgccca gcgcaacggg
     8341 ctgtgtgccc ggaaggacca tgacctgctg tggctgctgg ccccgctgct cagcgtgtgg
     8401 tcaagctggt ggatcgcgct gaccaagctc ggagctccgc cggagcagct cgccgcgctg
     8461 gagccgtggg agtacaccct cagctggctg gccacgccgg tgaccgtgac ggtcctggcc
     8521 gccgtcgaga tcaggaagct gcgggcggac ggcatcaggt tccgcaccag gaaccccgcg
     8581 cgcctccggc tcgcgagatg atcggcggta tgcgggaggt caccatccgc ccggcgaagc
     8641 gggacgagct ggccgccgtc gccgagctgc gctggcagtg gggcctggaa agggccatgg
     8701 agcacggcga ctcgcccggc accgggcacg aggagttcgt ggcccgcttc ctggactggg
     8761 ccggcaggca cgggcactcc caccattgcc tggtcgcggt ccgcgacggc ctggtcatcg
     8821 gcatggcgtg gctggcggtt ctccagcggg tgccgacccc gcttgccgtc gagcgggcat
     8881 ccggcgacct gcaatgcgtc tacgtcgtac ccggggaacg agacggcggc ctcggcggcc
     8941 tgctgatcca ggcggtgctg gcgctcgccc gcgagcttgg gctcgaacgg gtgacggtgc
     9001 actccagcat ccgggcggtt ccggcatacg cgcgccacgg gttcgcggcg tccccatgcc
     9061 tgctccaggc cgagcccgcc acctcgtcga ggtagtttcc ttgccgccgc gacatgcgaa
     9121 tatgaagcca tgccgaacac gctcaccctc accggcgacg ccgaagccga caagctgctg
     9181 tccgaggacc cgttcgccct gctcaccggc atgctgctgg atcagcagat cgccatggag
     9241 gtggccttcg ccgggccaag gaagatcgcc gaacggatgg acggcttcag cgtcaccagg
     9301 atcgccgagt ccgatctcga ggagttcgtg gaactctgcg tgcagaaacc ggccatccac
     9361 cgctacggcg gctcgatggc gcgccgggtg cacaccctgg cgcggcacat cgtggacagc
     9421 tacggcgggc gcaccgaggc gatctggacc gagggcgatc cggacggcac ggaggtgctg
     9481 aagcggctca aggcgctgcc cggctacggg gagcagaagg ccaagatctt cctggccctg
     9541 ctcggcaagc agctgggcgt ccagccggcg ggctggcgcg aggccgcggg agcctacggc
     9601 gaggacgggg cccgccggtc catcgccgat gtcaccggcc aggacaccct ggccgaggtc
     9661 cgcgccttca agaaggcagc caaagccgcc gccaagaagg gctgaccgcc catgagcggg
     9721 ccggcacaga caatgttcgc caccgaacaa ttccgccccg cgacgaacga ttacaagccg
     9781 tcacaaatct gatcaccaaa gagcgaacgc tagtcaatat gagcgaacca cggtgatccg
     9841 gagccgacgc caacatatcg caggttacca tatccaacta tgcaatcccg cgatagatct
     9901 agatcaaata tgtggaaggc acgatcgacc ccaagtaacc tgcaccggtt ggtgagtctc
     9961 cgattcccat acggacatat tccgcttcct gagtcccgcg aacgggcccg cgatgcgtat
    10021 ctctccctgg aacacgttca ggttctgcgc gacactcacc tctgttgtac ttctgggaat
    10081 ttgggtgaac ctcgtctcgg accgactttc gcgtggcgac ctgctgaact ccgtcgcgga
    10141 ccttggtccc ggaaatcttt tgttcgcagg gggagctgtt tcactcgtgg ggagcgagta
    10201 ccttacgcga aagaggcgca ccgtggaacg cgaacggttc gaacggcatc ttgaggtgct
    10261 tcggcaacgg cagcaacgac tactcaaaag cacgttgaag atcgtctgcg aactggtttc
    10321 caaagctctg gagattccat gcaacgggcg ctacttcgta gcgatcaagg acgactgcac
    10381 cacctacctt caacaagatc gggacctggc gatcctc
//
