LOCUS       NZ_CP076538            10822 bp    DNA     circular CON 16-DEC-2024
DEFINITION  Amycolatopsis aidingensis strain YIM 96748 chromosome, complete
            genome.
ACCESSION   NZ_CP076538
VERSION     NZ_CP076538.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis aidingensis
  ORGANISM  Amycolatopsis aidingensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 7657695)
  AUTHORS   Li,R.
  TITLE     YIM 96748 draft genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7657695)
  AUTHORS   Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2021) Yunnan University, Yunnan Institute of
            Microbiology, 121 Street, Yunnan 650000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdevono v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018885265.1-RS_2024_12_16
            Annotation Date                   :: 12/16/2024 14:59:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,118
            CDSs (total)                      :: 7,060
            Genes (coding)                    :: 6,941
            CDSs (with protein)               :: 6,941
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 44 of 119
            Pseudo Genes (incomplete)         :: 87 of 119
            Pseudo Genes (internal stop)      :: 2 of 119
            Pseudo Genes (multiple problems)  :: 13 of 119
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 14:56:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4315024
            Orig. end                         :: 4325846
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076538.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10822
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="22"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10822
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10822
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5822](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5822
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            708..2318
                     /locus_tag="KOI47_RS19815"
     CDS             708..2318
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240187.1"
                     /locus_tag="KOI47_RS19815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_269756724.1"
                     /sec_met_domain="Pkinase (E-value: 2.5e-51, bitscore:
                     173.2, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGHYRLVEHIGSGAMGVVWRAIDERLERSVAIKQILTQPGLSEA
                     ERVNIRQRAMREAKNAARFQHPNAIVVFDIAEHAGDPCLVMEYLPSQSLSAILAERPML
                     PVPEVAAIGKQVAAALVAAHRAGLVHRDVKPGNVLIDENGTAKITDFGISRAAGDITLT
                     QTGLIGGTPAYLAPELARGSDPAPSSDVFALGATLYHAIEGQTPYGNNANQLALLYSAA
                     NGKIIPPSNAGPATALLMSLLRTDPEERPSMAEARDQLTALATGGAAGAGGGGNGQKLS
                     ETLVSPPLMHAGRKPAEPASAPPPPVTGVQPPWQRTGKPAAPQPTSAFTPEGAAPAASP
                     SGGSSGGGGALGRRKPLVIGAAAVVLVVAATVLVILLNSGGGEDPGSDRQAQDPGTTAT
                     GTAPDGSQTSGSPSAPAGAPGTTPSQGTVAEGAAYDQLKAFLDAIGTEKAWEMLTPAAQ
                     SVYGDFSSFSQHWSDYALKDYETANEHGSDSDGAINVSYNIKYKDEGWDRSPVRIIDAG
                     GGKLLIDSDTRRPNARGGY"
     gene            2360..2659
                     /locus_tag="KOI47_RS19820"
     CDS             2360..2659
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439376.1"
                     /locus_tag="KOI47_RS19820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_216205580.1"
                     /transl_table=11
                     /translation="MADNGGPAGRRRFEDELAGLDPNDPEVQEFAAHLDRMERTGPSFT
                     VEGSIKGVGEFADASNRAGGGKWLAAVIVVCLILFGILFAAWDTIGRVLMWLSG"
     gene            2682..3107
                     /gene="msrB"
                     /locus_tag="KOI47_RS19825"
     CDS             2682..3107
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153937.1"
                     /locus_tag="KOI47_RS19825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_216205583.1"
                     /transl_table=11
                     /translation="MKPVVGATPRVVKSEQEWREQLGPEEYAVLRQAGTERPFTGEYTD
                     TETTGVYECRACGAELFRSTTKFASHCGWPSFYDPADSDAVLLREDRSMGMKRVEVLCA
                     SCHSHLGHVFEGEGYDTPTDQRYCINSVALKLVPTDS"
     gene            3129..3965
                     /locus_tag="KOI47_RS19830"
     CDS             3129..3965
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="KOI47_RS19830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_216205585.1"
                     /transl_table=11
                     /translation="MGEHWGLPGGAFLLLYVRLLNLPWITGMIMVRVWRRAGRHGEQLP
                     GPRHLAYLTGGPVQVVETTIAALLESGKLRVNSGGLFQAASGATGDDQLEDAVLENTRR
                     RRSPTRAGLVGSTRRSPGVLEIERYLARRGLVGAELPERRRTRMVLILQVLVLALGLGA
                     LLTSDGPENFVAILLMLILVGGGSAGGALFLQHSRRALDDVPTPAGRRLVAGSAAWPAT
                     RIRRSRPPRPRSSPPASDRRNAPSRRHRGWWRLWRRLRGRWLRRRRWRLRGLSRRR"
     gene            4048..4995
                     /locus_tag="KOI47_RS19835"
     CDS             4048..4995
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="KOI47_RS19835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_216205588.1"
                     /transl_table=11
                     /translation="MDQPWGVSGPAFLLLYGCLLVVPAVTVAVLLAASRRAAERRATEQ
                     ERLPSIYHLAYLLGGAERVVDTAIASLLEHGSLRVNSEGAIQPTGAVPAGDPVERAVYS
                     VASLHRRIRRRKLVHSVRRAPAVHDVRRQLEERGLLTAAWWRTRLLRILCAGYVLLAAL
                     GVLRLIAAVRVDARVDLLIVLLVVTGLLMVGSAVLARPRATGVPTPAGEGLAGRATARL
                     TPRTPTTPGARWSVGPIEGAVGAAAAAVVVDGLIGYPDEEIAEAVTWTPQPSFGGPIPA
                     HTDPGGFGLDTGAGEGGGGGGGGGGGGGGCGGGG"
     gene            5001..5822
                     /locus_tag="KOI47_RS19840"
     CDS             5001..5822
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671915.1"
                     /locus_tag="KOI47_RS19840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_408629837.1"
                     /sec_met_domain="DUF692 (E-value: 6.6e-84, bitscore: 280.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGSSGVPPLGVGIGWRPEIDLSVERLPGVDWVEVIAENLHSDRL
                     PESLVRLRARGLPVLPHAVSLSLGGAEPLDTRRVEHLATVAEALEAPLASDHVCFVRAG
                     GLDAGHLLPLPRTMDALDVLVANVRLAQSILGVPLALENIAALLEWPDPELSEGAFLRE
                     LTERTGCLLIVDVANLYAGARNLGSDPHRFLDELPLERIAYVHVAGGSEHEGVYHDTHA
                     APVPVPVLELLGELRARVRPPGALLERDDDYPTDAELAAELAAIRAVLEHR"
     gene            5819..6145
                     /locus_tag="KOI47_RS19845"
     CDS             5819..6145
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422375.1"
                     /locus_tag="KOI47_RS19845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_216205591.1"
                     /transl_table=11
                     /translation="MTARQRLARQQAQLLRALLAGERPPPGFPAERLRTEAAALRAKRR
                     RITEALRPDLAERLGERYRPLFEAYAAAHPRAEGTSARQDAENFAAWLETRRRPRWWRR
                     NRSR"
     gene            complement(6205..6897)
                     /gene="hemQ"
                     /locus_tag="KOI47_RS19850"
     CDS             complement(6205..6897)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /locus_tag="KOI47_RS19850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_216205594.1"
                     /transl_table=11
                     /translation="MARLNYQELNDTIRYTAWSVFRVEQGKLPEDRGASARETAEYLDA
                     LEDKGVAVRGVYDLAGLRADADYLIWWHAPEVEQVQAAYSGFRRTPLGRVSVPVWSQFA
                     LHRPAEFNRSHIPAFLAGEEPRRYVCVYPFVRSYEWYLLPDDERRKMLADHGKEARDYP
                     DVRANTVASFALGDYEWILAFEADELHRIVDLMRHLRGTEARRHVREEIPFYTGTRVPP
                     EELVLNLP"
     misc_feature    6990..7007
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.56"
                     /tool="antismash"
     gene            7056..10355
                     /locus_tag="KOI47_RS19855"
     CDS             7056..10355
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012311.5"
                     /locus_tag="KOI47_RS19855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="S8 family peptidase"
                     /protein_id="WP_216205596.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 7.5e-66, bitscore:
                     221.1, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQVRKRRSRPAAAVLAATTALVLGMAQPAGAEGGRAQAPSQAQLE
                     QQSVTLITGDRVLMRAGQPTGHLVAGPGREDITFTTFAADGHTYVVPSDAAPLIGTGVL
                     DRRLFDVTTLVRSGYADAERDSLPLLVTYADAPAAAGTMSAAEGTRITRRLPSIDGAAV
                     RAEKDSATALWDSITGAPGSMTTFDGGVRRVWLDGLRSTALDRSVPQIGAPAAWEAGYT
                     GAGTTIAVLDTGVDQTHPDLAERETAERNFTDAEDGADHVGHGTHVASIAAGTGAKSGG
                     TYRGVAPEADILDGKVLGDSGSGRESWIIAGMEWAAEQGADIANLSLGGGDSAEIDPLE
                     EAVNSLSAEHGTLFVIAAGNSGPASTTVGSPGTADAALTVGAVDREDEIAPFSSRGPRL
                     GDGGIKPDITGPGVGIVAALHSDGTIGEPVEDGYTALSGTSMATPHVAGAAALLAQQHP
                     GLSGAQLKRLLMASATPTEGASVFAQGAGRVDSAAALTQTVTAQPAGVNLGVQRWPHDD
                     DTPVGKEVTFANSGTEEVTLRLEVEATGPAGKPAPEGLFTLSATEVTVPAGGTAGVTVT
                     GDTRAAALDGSYSGSLVATAGEATVRTPLAINREVESYDLTLNAFDREGARTGQFSAFL
                     FGFADNRLRFPYAEDGSVTLRVPKGEYLLQGTVQSGEPGTTADLLAYPKLTVDGDLAVD
                     LDARRTKPVEIAAPQDSARPLLTEVSYTRRVHDSSFGSGFVALGEGLERLGTAHLGPQL
                     PPAELTALVSSQWLTEPAGEYYGLAWYEYGRVPTGLTKQVRPEDLATVRTSYGPIPDGL
                     RGVAGATPSPHLQPGGAWGTLIEIPESGPRIAHFTAEGVDWNRTLFLEDAESRRLHSRF
                     ESPPRSYQAGREYTESVNRAVFGPVMPESRFPYSWVIRDGDRITAQVPLFGDAAGNAGY
                     SATDSYSLRLYRDGEEVEPERGVFEVEPGTAGFRLTAEATRSNAELSTRVSAAWTFDSD
                     TTDRPSRLPVSTIRFFPDLDDANSAPGGRTFVFPVAVQEQGSPVTKVPHRLSAEVSYDG
                     GQTWRPAGVLGNSQLVVQHPAGADTVSLRAKATDGNGNTVEHTIINAYKLR"
     misc_feature    10712..10723
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
ORIGIN
        1 agctgcgtgc gctggtgcgg gacgcgggtc cggcggcgcc gctgctgttc ctttccgcat
       61 acgcggtgct caccgccgcg ccgatcccgc gcaccgtgtt cagcctggcc gcggggctgc
      121 tgctggggaa cctgctcggg ctggccgtcg ccatggccgg gaccgccgcc tcggccgcgc
      181 tgggcttcct ggccgccagg gcgctcggca gggacctggt cgccaggcac ctgcaccgga
      241 actgggtacg cacggtcaac gaccggctca ccggcggcgg cgtgctggcg gtgacctcgc
      301 tgcggctgat cccggtgatc ccgttcgccc cgctgagcta ctgctgcggg gtatccacga
      361 tccgcctcac gccctatgtg gcggggacgg ttctcggcag cctgcccgga acggccgcgg
      421 tggtgctgct cggcgacgcc atcaccggca ccaccccgcc cgcactgctg gcctgctacg
      481 cggtattcgc gctggccggg gcggtcggcg tggccagggt cgtacgcagg accaaccgta
      541 aagaagttga atccgaacgt accgccgtct gagcgtggcg ctggcgacga tccggatcgg
      601 gcgaccgcta cactcgatcg ggtgcgcgac cctgttgttc gcgcgcggtc aagccatgat
      661 tgcggatagt ttcccgagga gtaggcgtca tcgacaccgg tcagctcatc gcagggcact
      721 accgcctcgt agagcacatt ggcagcggcg ccatgggcgt ggtctggcgc gccatcgacg
      781 aacgcctcga gcggtcggtg gcgatcaagc agatcctgac gcagccgggt ctttccgagg
      841 cggagcgggt gaacatcagg caacgcgcca tgcgcgaggc caagaatgcc gcgcgcttcc
      901 agcatcccaa cgcgatcgtg gtgttcgata tcgccgagca cgcaggcgat ccgtgcctgg
      961 tgatggagta cctgccttcg cagagcctgt ccgcgatcct cgccgagcgg ccgatgttgc
     1021 cggtgccgga ggtcgccgcg atcggcaagc aggtcgccgc cgcgctggtc gccgcgcacc
     1081 gcgcgggcct ggtacaccgc gatgtgaaac cgggcaacgt gctgattgac gagaacggca
     1141 ccgccaagat caccgatttc gggatttccc gtgccgcggg cgatatcacc ctcacccaaa
     1201 ccgggctgat cggtggcacg cccgcgtatc tggcgccgga actggccagg ggctccgatc
     1261 ccgcgcccag ctccgatgtg ttcgcactcg gcgccaccct gtaccacgcc atcgagggcc
     1321 agacgcccta cggaaacaat gccaatcaac tggccctgct gtattcggcg gccaacggaa
     1381 agatcattcc gccgagcaac gccggaccgg ccaccgcact gctgatgagc ctgctgcgca
     1441 ccgaccccga ggaacggccc agcatggccg aggcgcgcga ccagctgacc gcgctggcca
     1501 cgggtggtgc ggccggggcc ggaggcggcg gtaacggtca gaagctgtcc gaaacattgg
     1561 tgtctccccc gctgatgcac gccggccgca agccagccga gccggcgtcg gcacccccgc
     1621 ctccggttac cggagtgcag ccaccatggc agcgcaccgg gaaaccggcc gctccgcagc
     1681 cgacctccgc gttcaccccg gaaggcgcgg cacccgcggc ctccccctcc ggtggcagta
     1741 gcggtggcgg tggcgcgcta ggcagacgta aaccgctggt catcggcgcg gccgcggtcg
     1801 tgttggtggt cgcggccacc gtgctcgtca ttctgctgaa ctccggcggg ggcgaggatc
     1861 ccggctcgga tcggcaggcg caggaccccg gcacgacggc caccggtacc gctccggacg
     1921 gatcacagac ctccggctca ccgtcggcac cggccggcgc ccccggcacg accccaagcc
     1981 agggaacggt tgccgagggc gcggcctatg accagctcaa ggcgtttctc gacgcgatcg
     2041 ggacagagaa ggcgtgggaa atgctgacac ccgccgcgca aagtgtgtac ggagatttct
     2101 cgagcttcag ccagcactgg tccgactacg cgttgaagga ctatgaaacg gcgaacgagc
     2161 acggttcgga ctctgacggc gcgatcaatg tctcgtacaa catcaaatac aaggacgagg
     2221 gttgggatcg gtctccggtc cggatcatcg acgccggagg cggaaagctg ctcattgatt
     2281 ccgacacccg gcggcccaat gcccgcggcg gctactgatt cggctgtccg gatggcgggc
     2341 ggtgccctac tgttgaggca tggccgacaa cggcggtccc gcgggtaggc gtcgtttcga
     2401 ggacgagctg gccgggctgg acccgaacga tccggaggtg caggagttcg ccgcgcacct
     2461 ggaccggatg gagcgcacgg gcccctcgtt caccgtggag gggtcgatca agggcgtcgg
     2521 ggagttcgcc gacgccagca accgcgcggg tggcgggaaa tggctcgccg cggtgatcgt
     2581 ggtctgcctg atcctgttcg gcatcctgtt tgcggcatgg gacacgatcg gtcgagtcct
     2641 catgtggctg tccgggtaac ctgggacata tgaaacccgg catgaaacct gtcgtcggcg
     2701 ccaccccgcg cgtggtgaag tcggagcagg agtggcggga gcagctggga ccggaggagt
     2761 acgcggtgct ccggcaggcc gggaccgaac gcccgttcac cggcgagtac accgacaccg
     2821 agacgaccgg ggtgtacgag tgccgcgcct gcggcgccga gttgttccgc agcaccacca
     2881 agttcgccag ccactgcggc tggccctcct tctacgatcc ggccgactcc gatgccgtgc
     2941 tgctgcgcga ggaccgcagc atgggcatga agcgggtcga ggtgctgtgc gcctcctgcc
     3001 acagccatct cgggcacgtg ttcgagggcg agggctacga cacgccgacc gaccagcgct
     3061 actgcatcaa ctccgtcgcg ctgaagctgg tacccaccga ctcctgaccg cacggccgaa
     3121 ggggcggaat gggcgaacac tgggggctgc cgggcggggc gtttctgctg ctctacgtca
     3181 ggctactgaa cctaccctgg atcaccggca tgatcatggt gcgggtgtgg cgccgggccg
     3241 gccggcacgg ggaacagctg ccgggccccc gacacctggc ctatctgacc ggcgggcccg
     3301 tgcaggtcgt cgagacgacg atcgccgccc tgctggagag cgggaagctc cgggtgaaca
     3361 gcgggggctt gttccaggcc gccagcggcg cgaccggcga tgatcagctg gaagacgcgg
     3421 tgctggagaa cacgcgccgc cgccggtccc cgacccgtgc cgggctcgtc ggatccactc
     3481 gccgctcgcc cggcgtgctg gagatcgagc ggtacctcgc gcggcgcggg ctggtcggcg
     3541 ccgaactacc ggagcggcga cggacccgca tggtcctgat cctgcaggtc ctggtactgg
     3601 ccttgggcct gggcgcgttg ctgaccagcg acggtccgga aaacttcgtc gccatcctgc
     3661 tcatgctgat acttgtcggc ggcggttccg ccggtggggc actgttcctc cagcacagcc
     3721 gccgcgcgct ggacgacgtc cccaccccag cgggccgcag gctggtcgcc ggttcggcgg
     3781 cctggccggc tacccggatc cgcagatcgc ggccgccgcg gccaaggtcg agcccaccgg
     3841 catcggaccg ccggaacgcc ccatcgcggc ggcaccgggg gtggtggcgg ctgtggcggc
     3901 ggctgcgggg gaggtggctg cggcggcgga ggtggcggct gcgggggctg agccggcgcc
     3961 ggtaacggtc tctcatcgac caccggaaac cgccgtccgg cccggcacgg ccctgggatc
     4021 ctggcgctat ccgcgggagg aggcggtatg gaccagccat ggggcgtttc cggaccggcg
     4081 ttcctgctgc tgtacggctg cctgctggtc gtgccagccg tcacggtcgc ggtgctactg
     4141 gccgcgagca ggcgcgcggc cgagcgccgc gccacggagc aggaacggct gccgagcatc
     4201 taccacctcg cctacctgct cggaggcgcc gaacgggtgg tggacacggc gatcgcgagc
     4261 ctgctggaac acggcagcct gcgggtcaac agcgagggtg ccatccagcc gaccggcgcg
     4321 gtcccggcgg gcgatcccgt ggagcgggcg gtgtactcgg tcgcctccct ccaccgtcgc
     4381 atccgcaggc gcaagctggt gcactccgtg cgacgggcac cggccgtaca cgacgtccgc
     4441 cgccagctgg aggaacgggg actactcacc gccgcctggt ggcggacccg cctgctgcgg
     4501 atcctgtgcg cgggctatgt tcttctcgcg gcgctcggag tactgcgcct gatcgccgcc
     4561 gtccgcgtgg acgcccgggt ggacctgctg atcgtcctgc tggtggtgac cgggctactg
     4621 atggtcggca gcgccgtcct cgcccggccc cgcgcgaccg gcgtcccgac tccggcaggg
     4681 gaaggcctgg ccggccgtgc cacggcacgc ctgacccccc ggacccccac gacaccgggc
     4741 gctcgctggt ccgtcggtcc gatcgagggc gcggtcggcg ccgccgcggc cgcggtggtg
     4801 gtcgatggcc tgatcggtta ccccgacgag gagatcgccg aagccgtcac ctggacgccg
     4861 caaccttcgt tcggcggccc catcccggcg cataccgacc cgggcgggtt cggcttggac
     4921 accggcgcgg gcgaaggagg cggcggtggc ggcggaggag gtgggggcgg gggcggctgc
     4981 ggtggtggcg ggtgagcccg gtgaccggat cctccggcgt gccaccgctc ggggtcggca
     5041 tcggctggcg cccggagatc gacctctcgg tggagcggct gcccggggtg gactgggtgg
     5101 aggtgatcgc cgagaacctg cactccgacc ggctgccgga gtcgctggtc cggctgcgcg
     5161 cacgcggcct cccggtgttg ccgcatgccg tatcgctctc gctcggcggg gccgaaccgc
     5221 tggacacccg cagggtggag cacctggcca cggttgccga ggcactggaa gcgccgctgg
     5281 ccagcgacca cgtctgcttc gtgcgcgcgg gtgggctgga cgcggggcac ctgctcccgc
     5341 tgccgcgcac catggacgca ctggacgtac tggtggcgaa cgtgcggctg gcgcagtcga
     5401 tcctcggcgt cccgctcgcc ctggagaaca tcgcggccct gctggaatgg ccggaccccg
     5461 aactgagcga gggcgccttc ctgcgggagc tgaccgagcg caccggctgc ctgctgatcg
     5521 tggacgtggc gaacctgtac gcgggcgccc gcaacctggg ctccgatccg caccggttcc
     5581 tggacgagct tccgctggag cggatcgcgt acgtgcacgt ggcgggcggt tccgagcacg
     5641 aaggcgtgta ccacgacacg catgccgctc cggttcccgt gccggtgctg gagctcctgg
     5701 gtgagctgcg cgccagggtg cgacccccgg gtgcgctgct ggaacgcgat gacgactatc
     5761 ccaccgacgc ggagctcgcc gccgagctcg ccgcgatccg ggccgtcctg gagcaccggt
     5821 gaccgcgcgg caacggctgg cacggcaaca ggcgcagctg ctgcgggcgc tgctggccgg
     5881 tgagcggcca ccgcccggct tcccggccga gcgcctgcgc accgaggccg cggccctgcg
     5941 cgccaaacgc aggcgcatca ccgaagcgct gcggccggac ctggcggaac ggctcggcga
     6001 gaggtaccgg ccgctgttcg aagcctacgc cgcggcacat ccgcgcgcgg agggcaccag
     6061 cgcacggcag gacgcggaga acttcgcggc ctggctggag acccggcgca ggccacgctg
     6121 gtggcggcgc aaccgcagcc gctaaggcag cgtttaagaa aagcgcgcac gcatcacctc
     6181 gagcagccat cttctaacca gctcctaagg caggttcagc accagttcct cgggcggcac
     6241 cctggttccg gtgtagaacg ggatctcctc gcgcacatgc ctacgggcct cggtgccgcg
     6301 caggtgccgc atcaggtcga cgatgcggtg cagctcgtcg gcctcgaagg cgaggatcca
     6361 ctcgtagtcg ccgagcgcga aggaggccac ggtgttcgcc cgcacgtccg gatagtcacg
     6421 ggcctccttg ccgtggtcgg ccagcatctt gcgccgctcg tcgtccggca gcaggtacca
     6481 ctcgtaggac cgcacgaagg ggtacacgca gacgtacctg cgcggttcct cccccgccag
     6541 gaaggccggg atgtggctgc ggttgaactc cgcgggccgg tgcagcgcga actggctcca
     6601 caccggcacc gagacccggc cgagcggggt gcgccggaac ccgctgtagg cggcctgcac
     6661 ctgctcgacc tccggggcgt gccaccagat caggtagtcc gcgtcggcgc gcaggccggc
     6721 gaggtcgtac accccgcgca ccgccacgcc cttgtcctcc agcgcgtcca ggtactcggc
     6781 ggtctccctg gcggaggcac cgcggtcctc cggcagcttc ccctgctcca cccggaacac
     6841 cgaccacgcg gtgtagcgaa tggtgtcgtt caactcttgg tagttcaacc gcgccatgtc
     6901 cccatggtcc cactccggcg tccgggccgg gcaccagccc acaccacgga acgtggggga
     6961 ttttccgacc ccgtaactca ctcggcggat tgtgacgcca ttgtgtatac cgatacgttc
     7021 gcgaaacttt cgcacccgat caggggaggt acctggtgca agttcgcaag agacgcagca
     7081 gaccggccgc ggcggtgctg gccgcgacca ccgcactggt cctcggcatg gcgcagcccg
     7141 ccggcgccga gggcggcagg gcacaggcgc cgagccaggc acagctggaa cagcagtcgg
     7201 tcaccctgat caccggtgac cgggtactga tgcgagccgg gcagcccacc gggcacctgg
     7261 tggcagggcc gggccgggag gacatcacct tcaccacctt cgcggccgat ggccacacct
     7321 atgtcgtgcc ctccgacgcg gcaccgctca tcggcaccgg cgtcctcgac cggcggctgt
     7381 tcgacgtcac cacactggtg cggtcgggtt acgccgacgc cgagcgggat tccctgcccc
     7441 tgctggtgac ctatgccgac gccccggcgg ccgcgggcac gatgagcgca gccgagggca
     7501 cgcggatcac ccggcggcta cccagcatcg acggcgccgc ggtacgggcc gagaaggact
     7561 cggcgaccgc cttgtgggac agcatcaccg gcgcacccgg ctcgatgacc actttcgacg
     7621 gcggggtacg cagggtctgg ctggacggcc tgcgcagcac cgccctggac cgcagcgtgc
     7681 cgcagatcgg cgcccccgcg gcctgggagg caggctacac cggggcgggc accaccatcg
     7741 ccgtactgga caccggcgtg gaccagaccc atcccgacct cgccgagcgg gagaccgccg
     7801 aacggaactt taccgacgcc gaggacggcg ccgaccacgt cgggcacggc acccacgtgg
     7861 cctcgatcgc ggccggcacc ggcgcgaagt ccggcggcac gtaccgcggg gtcgctccgg
     7921 aggcggacat cctggacggc aaggtgctgg gcgacagtgg ctcgggccgc gagtcctgga
     7981 tcatcgcggg tatggaatgg gccgccgagc agggcgcgga catcgcgaac ctgagcctcg
     8041 gcggcggaga cagtgccgag atcgatccac tcgaggaggc ggtgaactcg ctgtccgccg
     8101 agcacggcac cctgttcgtg atcgccgcgg gcaactcggg gcccgcgtcc accacggtgg
     8161 gctcgccggg caccgcggac gccgcgctca ccgtcggagc cgtggaccgc gaggacgaga
     8221 tcgcgccctt ctccagccgg ggaccccggc tcggcgacgg cggaatcaaa ccggacatca
     8281 ccgggcccgg cgtcggcatc gtggcggcgc tgcactccga cggcaccatc ggggaaccgg
     8341 tcgaggacgg ctacaccgcg ctgtccggga cctctatggc caccccgcat gtcgcggggg
     8401 ccgcggccct gctcgcccag cagcatcccg gcctctccgg cgcgcagctg aaaaggctgc
     8461 tgatggcctc ggccacgccc accgagggag cgagcgtgtt cgcccagggc gcggggcggg
     8521 tcgactcggc cgccgcgctc acccagaccg tcaccgcgca acccgccggg gtcaatctcg
     8581 gcgtccagcg ctggccgcat gatgacgaca ccccggtcgg caaggaggtc accttcgcca
     8641 actcgggtac ggaggaggtc accctgcggc tggaggtcga ggccaccggc ccggcaggca
     8701 agcctgctcc cgagggcctg ttcaccctct cggccaccga ggtcaccgtg cccgccgggg
     8761 gcacggcggg ggtgaccgtc accggcgaca cccgcgcggc cgcgctggat ggcagctaca
     8821 gcggcagcct ggtcgcgacc gcgggcgagg ccacggtgcg caccccgctg gcgatcaacc
     8881 gcgaggtgga gagctacgac ctcacgctga acgccttcga ccgcgagggc gcgcgcaccg
     8941 ggcagttctc cgcgttcctg ttcggcttcg ccgacaaccg gctgcggttc ccctacgcgg
     9001 aggacggcag cgtcaccctg cgcgtgccca agggcgagta cctgctgcag ggcacggtcc
     9061 agtccggaga acccggcacc accgcggacc tgctcgccta tcccaagctc accgtggacg
     9121 gggatctcgc ggtggacctg gacgcgcggc ggacgaagcc ggtggagatc gcggcaccgc
     9181 aggactcggc ccggccgctg ctcaccgagg tgtcctacac ccgcagggtg cacgactcga
     9241 gcttcggctc cgggttcgtc gccctcggcg agggcctcga acggctgggc accgcgcatc
     9301 tcggaccgca gttgccaccc gccgagctga cggcgctggt gagcagccag tggctcaccg
     9361 aaccggctgg cgagtactac ggcctcgcct ggtacgagta cggccgggtg cccaccggcc
     9421 tcaccaagca ggtgcggccg gaggacctgg ccaccgtccg caccagctac gggccgatac
     9481 cggacgggct caggggcgtc gcgggggcca ccccatcgcc acacctgcag cccggaggtg
     9541 cctggggcac gctgatcgag ataccggagt ccgggccacg gatcgcgcac ttcaccgccg
     9601 agggcgtgga ctggaaccgg accctgttcc tcgaggacgc cgagtcccgc aggctgcact
     9661 cccgcttcga gtcaccgccg cgctcctacc aggcgggtcg cgagtacacc gagtccgtca
     9721 accgggcggt gttcgggccg gtcatgcccg agagcaggtt cccgtactcc tgggtgatca
     9781 gggacggcga ccggatcacc gcgcaggtgc cgctgttcgg ggacgccgcg ggcaacgcgg
     9841 ggtactccgc gacggacagc tactcgctgc ggctgtaccg cgacggcgag gaggtcgagc
     9901 ccgaacgggg cgtgttcgag gtcgagcccg gcacggcagg gttccggctc accgcggagg
     9961 ccacccgcag caacgctgag ctgagcaccc gggtgtccgc ggcatggacc ttcgactcgg
    10021 acaccacgga ccggccgagc aggctgccgg tgtccacgat ccgcttcttc cccgacctcg
    10081 acgacgcgaa ctccgccccg ggcggccgga cgttcgtgtt cccggtggcg gtgcaggagc
    10141 agggcagccc ggtcaccaag gtgccgcacc ggctgagcgc cgaggtgtcc tacgacggcg
    10201 gccagacctg gcggcccgcc ggggtgctcg gcaactccca gctcgtggtg cagcaccccg
    10261 ccggcgcgga caccgtgtcc ctgcgggcca aggccaccga cggcaacggc aacaccgtgg
    10321 aacacaccat catcaacgcc tacaagctcc gctgactgcg ggccagcctg cgggcggcgg
    10381 catcggcggt ggcgacacac gccgggatgc cgacgccgtg caggctggcc ccggcgagtt
    10441 ccaacgcggg tacctcggcc accgcgcgct cgatgcgggc gacccggccg aggtgccccg
    10501 cgtcgtactg cggcagccca ccgccccacc gggtcaccaa agtgcgcagc ggttcggcgc
    10561 cgatcccggt gagctcggcc agatcggccc ggacccgccg caccagctcg gcgtcggtga
    10621 cccgcagcgt gccgttctcg ccgaagcggc cggccgagcc gcgcaccagc accgcatccc
    10681 cgtccctgtg atgcgcccac ttgcgggcgg agaaagtgaa cgccttggcg gtgaacggtt
    10741 ccccgtcccg gcgccgctcg gtcaccccga tcagcacgcc ggaggcatcg ggcagcacgg
    10801 ttgcgggcgg gagcgcgagg ga
//
