LOCUS       NZ_CP036145            10312 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Bacillus mycoides strain JAS94/5 chromosome, complete genome.
ACCESSION   NZ_CP036145
VERSION     NZ_CP036145.1
KEYWORDS    RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5275222)
  AUTHORS   Fiedoruk,K., Drewnowska,J.M. and Swiecicka,I.
  TITLE     The dynamics of pangenome and proteome of selected Bacillus cereus
            sensu lato representatives from the primary and anthropologically
            changes environments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5275222)
  AUTHORS   Drewnowska,J.M., Fiedoruk,K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2019) Department of Microbiology, University of
            Bialystok, 1J Ciolkowskiego, Bialystok 15-245, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 195.93x
            Sequencing Technology :: Illumina MiSeq and MiniIon Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018742285.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 04:27:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,786
            CDSs (total)                      :: 5,632
            Genes (coding)                    :: 5,433
            CDSs (with protein)               :: 5,433
            Genes (RNA)                       :: 154
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 82 of 199
            Pseudo Genes (incomplete)         :: 133 of 199
            Pseudo Genes (internal stop)      :: 60 of 199
            Pseudo Genes (multiple problems)  :: 66 of 199
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:45:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2290356
            Orig. end                         :: 2300668
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036145.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10312
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10312
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10312
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5312](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5312
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(70..86)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.99"
                     /tool="antismash"
     gene            761..1366
                     /locus_tag="EXW57_RS11705"
                     /old_locus_tag="EXW57_11985"
     CDS             761..1366
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853587.1"
                     /locus_tag="EXW57_RS11705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW57_11985"
                     /product="PRK06770 family protein"
                     /protein_id="WP_002127350.1"
                     /transl_table=11
                     /translation="MKTLFKIIGIIAGMAVIGVGVTYGMLYYLNNSKPAAKKASPAAPA
                     VEVLADSNVKAEDAKILENGNYSLPNSGFNKNFKWTDENIQTALHEMAHQKAKADQKWG
                     YIFITQERIESLLEIINSNDVAQKSTYVDILERWKQGKYEKVDVDHNKIWKLQSGNLGE
                     GKGVMSEKEQKELINQVFIQKGTYSGSKLIAGEVQANK"
     gene            complement(1472..2344)
                     /locus_tag="EXW57_RS11710"
                     /old_locus_tag="EXW57_11990"
     CDS             complement(1472..2344)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016095293.1"
                     /locus_tag="EXW57_RS11710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW57_11990"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_002127351.1"
                     /transl_table=11
                     /translation="MEMRHVKTFCAIVKYGSFSKAAHALGYAQSTVTAHIKALENDLNI
                     PLFDRLGKKILLTKAGHQFHPYALELLTIYDKAQEIPQNSDHLEGTLAITSNESLAVYR
                     LPQLLRTYKQENPKVNIVLETNTNEQALKKLHEGETDVIFIIGESIEHNDFITCNLCNE
                     TFGWIFPAHFPSNTDPLLLLQDTQFIFTEQSCGYRPMVDRFLRQSGRNPNKTFETSNVE
                     VIKQSVMCELGISILPYIVVQESNQKEQLCFQPIETPAVIQSHVIYHKSRWISPVLQSF
                     LTLLERIEK"
     gene            2474..3517
                     /locus_tag="EXW57_RS11715"
                     /old_locus_tag="EXW57_11995"
     CDS             2474..3517
                     /EC_number="1.2.1.11"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1219: N-acetyl-gamma-glutamyl-phosphate reductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200507.1"
                     /locus_tag="EXW57_RS11715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW57_11995"
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="WP_063226655.1"
                     /transl_table=11
                     /translation="MSEKSYHVAVVGATGAVGQKIIALLERVTKFNIEEITLLSSKRSA
                     GKIVQFKGREIKIQEAKSTSFEGVDIAFFSAGGEVSRQYVNHAVSSGAIVIDNTSEYRM
                     AHDVPLVVPEVNAHTLREHKRIIAVPNCSALQMVTALQPIRKSFGLERIIVSTYQAVSG
                     SGIHAIHELKEQAKSMLTGEEVKSTILPVKKDKKHYPIAFNVLPQVDIFTDNDFTFEEV
                     KMIQETKKILEDPNLKMAATCVRVPVVSGHSESVYIELEKEATVAEIKEVLLNAPGVTL
                     QDNPSEQIYPTPLYAEGKRDTFVGRIRKDPDTSKGFHLWIVSDNLLKGAAWNSVQIAET
                     LVGNGII"
     gene            complement(3764..4057)
                     /locus_tag="EXW57_RS11720"
                     /old_locus_tag="EXW57_12000"
     CDS             complement(3764..4057)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002168401.1"
                     /locus_tag="EXW57_RS11720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW57_12000"
                     /product="hypothetical protein"
                     /protein_id="WP_002127353.1"
                     /transl_table=11
                     /translation="MCNSKPIDELNIEDLKQNPIWEWAIDEAENEECDETWVKPVETIN
                     FTEELNGSIVLGELIIHNDEKFPMMCSIDIENNEVLISSIVFITKKKMSILL"
     misc_feature    complement(4288..4305)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.83"
                     /tool="antismash"
     gene            5001..5312
                     /locus_tag="EXW57_RS11725"
                     /old_locus_tag="EXW57_12005"
     CDS             5001..5312
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002189588.1"
                     /locus_tag="EXW57_RS11725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW57_12005"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_063226656.1"
                     /sec_met_domain="TIGR03601 (E-value: 4.8e-34, bitscore:
                     114.9, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNFQQELQALSLNDYQPGNIVYWDQQNQYPYYYITDDARRCGGC
                     GGGRCGGCGRCGGGRCGGFRCFGCFGCFSCFGCGGCSNCSNCSNCFDGFNGTTVTFEY"
     gene            complement(5621..6523)
                     /locus_tag="EXW57_RS11730"
                     /old_locus_tag="EXW57_12010"
     CDS             complement(5621..6523)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614455.1"
                     /locus_tag="EXW57_RS11730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW57_12010"
                     /product="LysR substrate-binding domain-containing protein"
                     /protein_id="WP_085963395.1"
                     /transl_table=11
                     /translation="MISIELRQLEYFLAVSKELHFTKAAEKLNISQPSLSQQIRALEHE
                     VGMPLFDRIGKKISLTEAGRVLLSHSKTIFHEVEQARSAIQDLNGLHHGSLTIGSLLTV
                     VNYLLPPAILDFNKQYPNIELSVLGLRTGEIREKLLQNELDIGITFLPVQDKEIVSIPL
                     YKSELTLVVPTSHSLTERKYVSIEELQDYPLILLPKNFYLTELITSHCQNFNFKPKPIL
                     EISTMESLIQMVAQGMGITVLPKPYIDFLQNEHIQAIKIENPTPTIDIGLIYRRDKYMC
                     AATRKFIEQLKITVHSLQK"
     gene            6653..7978
                     /gene="gabT"
                     /locus_tag="EXW57_RS11735"
                     /old_locus_tag="EXW57_12015"
     CDS             6653..7978
                     /EC_number="2.6.1.19"
                     /GO_function="GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (34-374). E-value: 4.7e-122.
                     Score: 399.2. Matches aSDomain:
                     nrpspksdomains_EXW57_RS11735_Aminotran_3.1"
                     /codon_start=1
                     /gene="gabT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200502.1"
                     /locus_tag="EXW57_RS11735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW57_12015"
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_002031963.1"
                     /sec_met_domain="Aminotran_3 (E-value: 9.9e-132, bitscore:
                     437.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKSSVLPGSYSQVLQKRYEEAVPTGIYHLTPLYVKEARGAIITD
                     VDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAESFIHPCFHVTPYENYITLAEKINEK
                     VPGTSKKKTMFANSGAEAIENAVKIARKVTGRSKVISFERGFHGRTLMTMSLSTKETPY
                     KAGFGPFVSDMHILPYPYYYRAEEGVSPEEVDSQVIYQFEKFLEEVSPEKVAAIVMEPV
                     QGEGGFIVPSISFVKRLREICNQYGILMIMDEVQTGFGRTGKWFATEHFHIEPDIMTLS
                     KSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGSPLSCAAGLAVFEIMEEENLVERAQQ
                     IGNRIMSHLHQMQLKYEMIGDVRGIGAMVAMELVTNRITKEPAKELTVKLIEDFWKNGL
                     ISIGAGIFDNVLRFLPPLVISNEEIDRGFEIINESFEKLCRK"
     aSDomain        6755..7774
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EXW57_RS11735_Aminotran_3.1"
                     /evalue="4.70E-122"
                     /label="EXW57_RS11735_Aminotran_3.1"
                     /locus_tag="EXW57_RS11735"
                     /protein_end="374"
                     /protein_start="34"
                     /score="399.2"
                     /tool="antismash"
                     /translation="VKEARGAIITDVDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAE
                     SFIHPCFHVTPYENYITLAEKINEKVPGTSKKKTMFANSGAEAIENAVKIARKVTGRSK
                     VISFERGFHGRTLMTMSLSTKETPYKAGFGPFVSDMHILPYPYYYRAEEGVSPEEVDSQ
                     VIYQFEKFLEEVSPEKVAAIVMEPVQGEGGFIVPSISFVKRLREICNQYGILMIMDEVQ
                     TGFGRTGKWFATEHFHIEPDIMTLSKSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGS
                     PLSCAAGLAVFEIMEEENLVERAQQIGNRIMSHLHQMQLKYEMIGDVRGIGAMVAMELV
                     "
     gene            7993..8682
                     /locus_tag="EXW57_RS11740"
                     /old_locus_tag="EXW57_12020"
     CDS             7993..8682
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262982.1"
                     /locus_tag="EXW57_RS11740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW57_12020"
                     /product="MOSC domain-containing protein"
                     /protein_id="WP_199627500.1"
                     /transl_table=11
                     /translation="MTKVYEILSLNLGLPKEVMYGGKLIQTGINKKQVKEPVFLSFLKF
                     NGDGQADLVHHGGVDKAVCVYTGDHYPYWEKELNQELVYGAFGENITVSGMCEEDVCIG
                     DTFQLGEAIVQVTQPRQPCFKLAKKYNIPKLPLYFQDTGYTGFYFRVLKEGWILPVDTL
                     KLLKLDPKGVSILFANRIMHKEKQNSEGLKRILEVNALSTSWRKTFEKRVKGEEISTKE
                     RLEGTKE"
     gene            8945..9829
                     /locus_tag="EXW57_RS11745"
                     /old_locus_tag="EXW57_12025"
     CDS             8945..9829
                     /EC_number="2.3.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526760.1"
                     /locus_tag="EXW57_RS11745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW57_12025"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_199627502.1"
                     /transl_table=11
                     /translation="MGNVEKMANFLLEEPRREQLFPLFEEVFGITSQTLQDFSEKGYWD
                     YTYKALSFLQDDNAIANVAAFSLPLLINGEKINATGIQSVMTHPNYRRRGLMKQLFSKM
                     IEEIDEQCGCAVLFTENPELYKPFGFQIVQEYLMTVSYENKGYKDSQLKKLDFYNEEDR
                     QLIHEIIENGQRLSNKFSTLNYRSSFYLNMYDSTWNENLYYSEKLDALIVFTVENQKLK
                     LFGVFAPVIPILDELCEEISGEFTEIEFYFYPDQLGIDDVQYTELQTSKYLMVRSNKEL
                     DFKGYKFPVLTEF"
     misc_feature    complement(9913..9929)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.8"
                     /tool="antismash"
ORIGIN
        1 tggtattttg aaaaacaagt agagcgctgg aaaatagcaa tacagaagtc tcagcaatat
       61 gaattagtaa tactcgatgg ggatatatat cagccactta gttataattg gtgttttcgt
      121 tttgatatat ttaatcagcc gttatcttta atagaaaact tttataaaga aaagatgata
      181 aatagagaaa ttggttttcc agatcaatat ttttatttat atacaagtga tgaagagctt
      241 agaaaacgga aagagtttga tgggacgaaa aaaagaagaa actttgaaaa acatttacat
      301 atatctaaag catttcagcg ttattatgaa aatctcaata ccgtcacaga cgggtactgt
      361 aaattgattg aagcaaaaag tataaagtcg aatgaattag aaattgtgaa aagtttaaac
      421 agcttaaaag tatgtgaaga aagacgtttt gatgtttcga ggttagatgc tattacggag
      481 tggttaaaag aaaatcgtgc ataaagtaaa aacaacgagt gttttagaag gcactcgttg
      541 tttttttgtt tccatataaa tataaaggaa aattttgtag aatatcttta ttttttaaca
      601 gaataatctt tcaatttcac gaaaaaagaa atacaatata aggaaatata tataaataat
      661 atgatagggg atgggatgaa ctattttgaa tgaaggaact gttttatata agaaataatg
      721 aggtagtaag aacagagata tttgggagga aacaaacaag atgaaaacat tatttaaaat
      781 cataggaatt atagctggta tggcggtaat cggggtaggt gtaacgtatg gtatgctgta
      841 ttatctgaat aatagtaaac cagctgcgaa aaaggcatca ccagccgctc cggcagtaga
      901 agtgctagct gatagtaatg taaaagccga agatgcaaag atattagaaa atggaaatta
      961 ttcattgccg aatagtggtt ttaataaaaa cttcaaatgg acagatgaga acatacagac
     1021 agcattacat gaaatggcac atcaaaaagc gaaagctgat caaaaatggg gctatatttt
     1081 tattacacaa gaacgtattg aaagtttact tgaaattata aatagtaatg atgttgcaca
     1141 aaaaagtaca tatgtggata ttttagaacg atggaaacaa ggaaaatatg aaaaggttga
     1201 tgtggaccac aacaagattt ggaaattaca aagtgggaat ttaggagaag ggaaaggagt
     1261 aatgtcagaa aaggagcaaa aagaattaat taatcaagtt tttatacaaa aaggtacata
     1321 ttcaggtagt aaattaattg caggtgaggt acaagctaat aaataatata catattgatt
     1381 ttgaaacacc tgtattgtta tgaacacaaa tgttttaaca aaaatgagta attgcttttt
     1441 agagcagtta ctcatttttg taattactcg cttacttttc tattctctct aataatgtaa
     1501 gaaacgattg caaaactggg gatatccatc tagatttatg atagattacg tggctttgaa
     1561 tgacggctgg agtttcgatg ggctgaaaac agagttgttc tttttgatta ctttcttgta
     1621 caacgatgta agggagaata gaaattccta actcacacat aacggattgt ttaatgactt
     1681 caacattgga agtctcaaat gttttattag gatttcttcc gctttgacgt aaaaaacgat
     1741 ctaccattgg tctatagcca cagctttgtt ctgtaaatat aaattgagta tcttgtaata
     1801 aaaggagtgg atcagtgttt gaagggaaat gagcagggaa aatccatcca aatgtttcat
     1861 tacataaatt gcacgtaata aaatcattat gctctatact ttctccaatt atgaagataa
     1921 catcggtttc tccttcatgt aattttttca atgcttgctc attggtattt gtctcaagta
     1981 cgatatttac tttcggattt tcctgtttgt aagtacgtaa taattgtggt aaccgataca
     2041 ctgccaatga ttcattagat gtaatagcca atgttccttc taaatggtca ctgttttgag
     2101 ggatttcttg cgctttatca taaatagtaa gtaattctaa tgcatacgga tgaaattgat
     2161 gacctgcttt tgtaagaagt attttttttc ctaatcgatc gaatagggga atgtttaaat
     2221 cgttctctaa tgctttaata tgggctgtca cagtagattg cgcataacct aatgcatgag
     2281 cagctttaga aaagctacca tatttaacaa tggcacaaaa tgttttaaca tgtctcattt
     2341 ccataaatga tacctctttt attatcatga tttgtgaatg attatatcat gtatttcaat
     2401 ttttcaaatc attaaatttc ttttaaaatt aagttgtgga gaaattcatt caattgaaat
     2461 tggaggagaa aaaatgagtg aaaagagcta tcatgtagct gtagttggag ctacaggcgc
     2521 agtagggcaa aaaatcattg cattgttaga gagagtaacg aaatttaata tagaagaaat
     2581 tacattactc tcgtcaaaac gatctgctgg taagatagta caatttaaag gacgagagat
     2641 taaaatacaa gaggcaaaat caactagttt tgaaggtgta gacattgcct tttttagcgc
     2701 tgggggagaa gtttctagac aatatgtaaa tcatgcagtt tctagtggtg caatcgtaat
     2761 tgacaacaca agtgaatacc gaatggcaca cgatgtacca ctcgtcgttc cagaagtaaa
     2821 tgcacatact ttaagggaac ataaaaggat cattgcagtt ccgaattgct cagcactaca
     2881 gatggtaaca gctcttcagc caattcgaaa atcatttggt ttagaacgaa ttattgtttc
     2941 aacgtatcaa gccgtatcag gctcaggaat tcatgcgatt catgaattaa aagaacaagc
     3001 taagtcaatg cttacaggtg aagaagtgaa gagcactata ttaccagtga aaaaagataa
     3061 aaaacattat ccaattgcat ttaatgtatt gccgcaagtg gatatattta cagacaatga
     3121 tttcacattc gaagaagtaa agatgattca agagacgaag aaaattttag aagatccaaa
     3181 tctgaaaatg gcagctactt gcgtcagagt tccagttgta tcggggcatt ctgaatcagt
     3241 ttatattgaa cttgaaaaag aagcgaccgt tgcagaaatt aaagaagtac tactgaatgc
     3301 accaggtgtt actttacaag ataatcctag tgaacaaatt tatccaacgc cattatatgc
     3361 agagggtaaa agagatacat ttgtaggtag aatacgtaaa gatcctgata catcaaaagg
     3421 attccatctt tggatcgttt ctgataattt attaaaaggt gctgcttgga actcagttca
     3481 aattgcagag acattggtgg gaaatgggat tatttagtat ggatttatat aaatattgaa
     3541 aatagaagga tatttgggaa accgttaatt ataatggttt cctgtttttt acttaatgag
     3601 tattttcctc ttacttcaat taaaatttaa tttaagttag tttttatagt gtttttataa
     3661 atatcaactt tatctgcaga gaattttaaa aatctaggct ctccgttaat tgtgagatta
     3721 atattaagtg ataaaggcat ttcaaccttt ttcactacat cttctatagc aatatactca
     3781 tcttcttttt cgttataaaa acaatggaac taattaatac ctcattattt tcaatatcaa
     3841 tactacacat catagggaat ttctcatcat tatgtattat taattcgcca agtactattg
     3901 aaccgtttaa ttcttctgta aaatttattg tttccactgg ttttacccac gtttcatcgc
     3961 actcttcatt ttccgcttca tctattgccc attcccatat tgggttttgt ttcaggtcct
     4021 caatatttaa ttcatcaatt ggtttgctat tacacattaa tatatactcc ttttgccatg
     4081 aaaatggatg agtaaaaaat ttattcatta attgtaaaat gtggctattt gcaatcctat
     4141 aaatacaagt ttgctcgttt caataaatag ttgatcgcaa ataagaagtt tcgatgtaga
     4201 gtattatcca tttgatatta ttaaatctag ttttgtaatg ttgaacttat ttttacaacc
     4261 tattaaaagt ctattcttac agtaatctta acaaggtttt aactagacag taaataatgc
     4321 cttttgaaaa ggaaaaccgt ggttgtgatg ggggaaatag caaaaaacat cgctgatttg
     4381 ccattcataa aaatagactt ttcttagcag aaatcactaa ttataaatac ttataaaatt
     4441 agctctgata tagcagatat tgaagcgaag aaaaataaag atgggagtgc atattggaaa
     4501 tacgaagatg accaaacaaa attatggaat atacgactat agaactagaa aatgatgaca
     4561 taaatctaaa tattattgtt taaattcata ttttataatg tccatttatc ccgcattaac
     4621 tgcccgtaaa ccccctctga ttaaagtttc actttataaa aatgagccca tagtaaaaat
     4681 aacctaaaaa taggcatatg aacaaggtgc atcatctagt gataggtcat attttgttgg
     4741 tgtacgtatt gaacttagtt tatcccgcta tttgtgggca gtaaaactcc cacctcaaaa
     4801 ttcaactgga ggaaagaagt taggtgggag ccctgctgcc cgtaaacgcc cgattggtgc
     4861 gggctaataa tcagtggggg atgaacaaaa cccctactga ttaaagtttc actttatagc
     4921 gctatttcta cttagattta acgaacagaa atataggaaa gtatcgatgg ggttttagtt
     4981 tataaacgga ggtcatgaag atgaataatt ttcaacaaga actacaagcg ttaagcctta
     5041 atgattatca gcctggaaat attgtgtatt gggaccagca aaaccaatat ccatattact
     5101 atattacaga tgatgctcgt cgctgtggcg gctgtggcgg tggtcgttgt ggtggttgtg
     5161 gtcgttgtgg cggtggtcgt tgtggtggat tccgttgttt tggttgcttc ggttgcttta
     5221 gttgttttgg ttgtggtggt tgctctaatt gctctaattg ctctaattgt tttgatggtt
     5281 ttaatggtac tactgtaaca tttgaatatt gataagagac aaaaaggaat ccattttgaa
     5341 aaaagattga tcgaaacgga ttcctacttt gtgaagcatt atataatttt tataaaaatg
     5401 tgctaacaag tttgcaaacg gacttgctaa cacctatacg aaatagacat aaaatatcgc
     5461 tgatgtgtag cttaataata cgataataat ttagcactta taatgaataa taggttatta
     5521 gaaaagaaac cataaaatgt aatgttttat atcattataa aaagagatta ttctaatagg
     5581 tcgttgtgta accgctgcat aggataatct ctttttatgc ttatttttgt aaagaatgta
     5641 ctgttatttt gagttgctca ataaatttcc gggtagcagc gcacatatat ttatctcttc
     5701 tataaataag tcctatatct attgttggag tggggttttc aattttaata gcttgtatgt
     5761 gctcattttg caaaaagtct atatacggtt ttggtaacac ggtaatcccc ataccttgtg
     5821 cgaccatttg aattaaagat tccattgtac taatttctaa aattggcttt ggtttaaagt
     5881 taaagttttg gcaatgagat gtaattagtt ctgttaaata gaaattttta ggtagaagaa
     5941 ttaaaggata gtcctgtaat tcttcaatag atacatactt acgttcagtt aaagaatggc
     6001 tggttggcac aactaatgta agttcacttt tatatagtgg gatagaaaca atctctttat
     6061 cttgcaccgg gagaaatgta ataccaatat ctaattcatt ttgtaataat ttttcacgaa
     6121 tttctcctgt acgaagcccc aacacagaga gttcgatatt tggatattgc ttattgaaat
     6181 ctaatatagc tggtggtagt aaataattta ctacggttaa caaagatcct attgttaagg
     6241 aaccatgatg taatccattt aaatcttgaa tggcagaacg agcttgttcg acttcgtgaa
     6301 agattgtttt actgtgcgat aataaaactc ttcctgcttc agttaaagat atttttttac
     6361 caattcgatc gaaaagtggc atgcctactt catgttctaa tgcacgtatt tgttggctta
     6421 gtgaaggttg tgaaatattt aatttctcgg ctgcttttgt gaaatgtaat tctttcgaaa
     6481 cagctaaaaa gtattctaat tgtcgtaatt cgattgaaat cacctcaagt ttaatcatag
     6541 gatataccta tcattattat ataaataatt gaattgaaca atggttttgc gggatatata
     6601 atcactttat cagaaatgga ttgattaaaa aattagatga ggtgaagaag aattgttgaa
     6661 atctagtgta ttaccgggca gttattcaca agtacttcaa aaaaggtatg aggaggccgt
     6721 accgacagga atatatcatt taacgccttt atatgtaaaa gaagcaaggg gagcgataat
     6781 aacagatgta gatggaaatc agtttattga ttttgctggt ggaattggta tgcaaaatgt
     6841 tggacattgt catccgaaag ttgtaagagc ggtacaaaaa caagcagaat catttataca
     6901 tccttgtttt catgtaactc catatgaaaa ttatattact ttagcggaga aaattaatga
     6961 gaaagtgcca ggtacaagta agaaaaaaac gatgtttgcc aatagtggtg cggaagcgat
     7021 tgaaaatgct gtaaagatcg cacgtaaagt aacagggagg agtaaagtaa tttcgtttga
     7081 acgaggattt catgggagaa cattaatgac aatgtctcta tcgaccaaag aaactccata
     7141 taaagcagga tttggtccgt tcgtttcaga catgcatata ttgccgtatc cgtactatta
     7201 cagagctgaa gaaggagttt ctcctgaaga agtggattct caagtcatct atcagtttga
     7261 aaagttttta gaagaagtat cacctgaaaa agtagcagcg atcgtaatgg aaccagtaca
     7321 aggagaaggt gggtttattg ttccttccat ttcatttgta aaacgattac gagaaatttg
     7381 taatcaatac ggtattttaa tgataatgga tgaagtgcaa actggatttg gccgaacagg
     7441 gaaatggttt gcaacggaac attttcatat agaacctgac ataatgactt tgtcaaaatc
     7501 aattgctggc ggtttaccat taagtgctat tgtcggtcgt gcggagttga tggacattcc
     7561 tgggagagga caaattggtg gaacttttgc cgggagtccg ttatcatgtg cagccggtct
     7621 tgctgtattt gaaataatgg aagaagaaaa tctagttgaa agggctcagc aaattggaaa
     7681 tagaataatg tcacatctac atcaaatgca attaaagtac gaaatgattg gtgatgttcg
     7741 tggaatcggt gcgatggttg caatggaact tgtaacgaat cgcataacga aagaacctgc
     7801 aaaagaacta acagtaaagc taattgaaga tttttggaag aatgggctta taagtattgg
     7861 agcggggata tttgataatg tactacgctt tttaccacct ctagttatct caaacgaaga
     7921 aatagataga ggatttgaaa taataaatga gtcttttgaa aagttgtgcc gcaagtaaga
     7981 ggagggattt gaatgactaa agtatatgaa atcctatctt taaatctagg attgccaaaa
     8041 gaggttatgt atggtgggaa gttaattcaa acagggataa ataaaaagca agtaaaggaa
     8101 cctgtttttt tatcattttt aaagtttaat ggagatggac aggcggattt agttcatcat
     8161 ggaggggtag ataaagcggt ttgtgtttac actggtgacc actatcctta ctgggaaaaa
     8221 gagttgaatc aggaacttgt atatggggct ttcggtgaaa atataacagt aagcggaatg
     8281 tgtgaagaag atgtttgtat tggtgacacg tttcaactcg gagaagcgat tgtacaagta
     8341 acgcagccaa gacaaccttg ttttaaatta gccaagaaat ataatattcc aaagttgcca
     8401 ttatattttc aagatacagg atacacaggt ttttattttc gagtattaaa agaagggtgg
     8461 atattaccgg tagatacttt aaaattgttg aagcttgatc caaaaggtgt gtcaattttg
     8521 tttgcgaatc gcataatgca taaagaaaaa caaaactctg aaggattaaa aaggatttta
     8581 gaagtaaatg cactgtctac tagttggaga aagacgtttg aaaagcgagt gaagggagaa
     8641 gaaattagta cgaaggagcg acttgaggga accaaagaat aggtgaatta tatttgcata
     8701 aaatggtgta tttctattat cggaaagata agtaggaagc accatttgtt atataataaa
     8761 catgatatta ttcaaaaatc ttaatactag tttattcgac ttgtttttta ataatcaaaa
     8821 cataaaataa attaaaccat ttttaaaagt tttatgatac tatattgatt tgaaaggcct
     8881 attttttgag agtaggtcat ataagtatat ctaaattaat ggaatcgacg tatggaggga
     8941 caacatgggc aatgttgaaa aaatggcaaa ctttctatta gaagaaccga gaagagaaca
     9001 gttatttcct ttatttgaag aagtgtttgg gattacgagc caaacattgc aggatttttc
     9061 agagaaaggg tattgggatt atacatataa agcattatca tttttacaag atgataatgc
     9121 gattgcaaat gttgcggcat tttcacttcc gcttttaatt aatggtgaaa aaataaatgc
     9181 aacgggtatt caatcagtaa tgacacatcc gaattatcgt agacgaggat taatgaagca
     9241 attgtttagt aagatgatag aggaaattga tgagcaatgc gggtgtgcgg tcttgtttac
     9301 tgaaaatccg gaactatata aaccatttgg ttttcaaatt gtgcaagaat atttgatgac
     9361 agtatcatac gaaaataaag gctacaaaga ttctcaactt aaaaagttag atttttataa
     9421 tgaagaggat agacaattaa tacatgagat tattgaaaat ggccaaaggc tttctaataa
     9481 attttcaacg ttaaattacc gctcttcgtt ttatttaaat atgtatgatt caacatggaa
     9541 tgaaaacctt tattattcag agaaattaga tgcgctgatt gtttttacag tagaaaatca
     9601 aaaactaaaa ttatttggag tatttgcgcc ggtaattccg attttagatg aattatgtga
     9661 ggaaatctct ggggaattta cggagattga attttatttt tacccagatc aattaggaat
     9721 tgatgatgta caatatacag aattacagac tagtaagtat ttaatggttc gaagtaataa
     9781 agaattagat tttaaaggtt ataaattccc ggtattaact gagttttaaa tattaattta
     9841 aatgcttttt tatggaggaa ttatgaaaac atcatatata ccaaatcata ttgagcaact
     9901 tactgtaatg aacgcaccta aattttacaa acttgaagac aatgatacat tcattgtcca
     9961 ttcgaaagaa gaaacagatt tttggctgaa aacccattat ggttttgaag taatgaatgg
    10021 ccatgtattt tatgatgaga taatgacaga ttttcaggct gaggttcaat tgcgcatgaa
    10081 tccaaatgcg acatacgatc aagcgggttt atttgttatg atttctgaga agtgttggtt
    10141 aaaaacatct ttagaatata ttcctgaggg cccatcacat ttaggtgctg tcgtaacgaa
    10201 taatggctat tctgattggt caacgcaaga ctatccaaat gagtttgcta agcaacaatt
    10261 acggtttcga attattcgaa aacttggcga ttatacaata tatgtgaaaa cg
//
