LOCUS       NZ_CP036117            10276 bp    DNA     circular CON 12-DEC-2024
DEFINITION  Bacillus mycoides strain JAS481 chromosome, complete genome.
ACCESSION   NZ_CP036117
VERSION     NZ_CP036117.1
KEYWORDS    RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus; Bacillus cereus group.
REFERENCE   1  (bases 1 to 5296574)
  AUTHORS   Fiedoruk,K., Drewnowska,J.M. and Swiecicka,I.
  TITLE     The dynamics of pangenome and proteome of selected Bacillus cereus
            sensu lato representatives from the primary and anthropologically
            changes environments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5296574)
  AUTHORS   Drewnowska,J.M., Fiedoruk,K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2019) Department of Microbiology, University of
            Bialystok, 1J Ciolkowskiego, Bialystok 15-245, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 187.86x
            Sequencing Technology :: Illumina MiSeq and MiniIon Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018741645.1-RS_2024_12_12
            Annotation Date                   :: 12/12/2024 04:46:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,189
            CDSs (total)                      :: 6,036
            Genes (coding)                    :: 5,734
            CDSs (with protein)               :: 5,734
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 302
            CDSs (without protein)            :: 302
            Pseudo Genes (ambiguous residues) :: 0 of 302
            Pseudo Genes (frameshifted)       :: 122 of 302
            Pseudo Genes (incomplete)         :: 192 of 302
            Pseudo Genes (internal stop)      :: 81 of 302
            Pseudo Genes (multiple problems)  :: 78 of 302
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:51:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2607103
            Orig. end                         :: 2617379
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036117.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10276
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10276
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10276
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5276](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5276)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    19..33
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.81"
                     /tool="antismash"
     gene            complement(972..1415)
                     /locus_tag="EXW43_RS13635"
                     /old_locus_tag="EXW43_13990"
     CDS             complement(972..1415)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="EXW43_RS13635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_13990"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_002085308.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDETHEKLLMVKNKGENGSYYTLPGGAVKFGETL
                     EEAAIREVKEETGLDISVKGVCSISEAFFEERGHHAIFFNFLGEITGGEICISRPKEIE
                     EITWMELDSAEPYLRIPEHLKSLLQKKETVPYIFNGTIIHQSS"
     gene            complement(1600..2016)
                     /locus_tag="EXW43_RS13640"
                     /old_locus_tag="EXW43_13995"
     CDS             complement(1600..2016)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016104977.1"
                     /locus_tag="EXW43_RS13640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_13995"
                     /product="N-acetyltransferase"
                     /protein_id="WP_002085307.1"
                     /transl_table=11
                     /translation="MQIVKQWVQEDSDYIREKVIEYNQKQLSNEEKTPSEKISFIVKNE
                     KEEIVGGITAITFWHHVYVDFLWVSEEYRHEGYGSNLMNLIEEFAIKKECRLIKLDTFS
                     FQAPNFYKKHGYKVIGVSEDYPKGHNHYYLEKRL"
     gene            complement(2197..3405)
                     /locus_tag="EXW43_RS13645"
                     /old_locus_tag="EXW43_14000"
     CDS             complement(2197..3405)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="EXW43_RS13645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_14000"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_078179189.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAGKLQQKFGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYVNGNLINSLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNTATETLHNDYTPREMMKTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAIFNTIGRIILGTLSDKIGRLKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFQPTFITIGVLCVISFVISIVIRPPNAEKKKEIKSLNR
                     KIA"
     gene            complement(3684..3827)
                     /locus_tag="EXW43_RS13650"
     CDS             complement(3684..3827)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="EXW43_RS13650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002013225.1"
                     /transl_table=11
                     /translation="MKNEVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFEEI
                     EY"
     gene            complement(3859..4887)
                     /locus_tag="EXW43_RS13655"
                     /old_locus_tag="EXW43_14005"
     CDS             complement(3859..4887)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002085304.1"
                     /locus_tag="EXW43_RS13655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_14005"
                     /product="serine hydrolase"
                     /protein_id="WP_144513092.1"
                     /transl_table=11
                     /translation="MYKYEKLISWVESIKETNQSSAAALCIIKDNKIVLEHYSGTHSNT
                     PTSKKVTISSQFNVASARKSYLGLMVAYAVYEGIIKSLDDEAVNYFKEYDPSLLGKTTI
                     RHLVTHSHGLNETDDGTAFREFEPGQAWAYRDINVRMVTQLIYQLYNKSFPELLKERVF
                     TPADFKKTGWRIKQDDNLVKVVVNPDEDAISSVGAVDDGSEKNLFVSAREFAFWGNLHL
                     NKGFLNGKQIVPKEVIALATSLQSPEYTNKELPQNGLFWFVQNEPALFSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSSSKNTMV
                     RV"
     gene            complement(5001..5276)
                     /locus_tag="EXW43_RS13660"
                     /old_locus_tag="EXW43_14010"
     CDS             complement(5001..5276)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001869.1"
                     /locus_tag="EXW43_RS13660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_14010"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001001869.1"
                     /sec_met_domain="TIGR03601 (E-value: 7.4e-39, bitscore:
                     130.3, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNEFQQELQALNLNAYQPGNVVYWDQQQNQYPYYYIQDDARRCGG
                     CGGGGRCGGCGGRCGGCGGGRCGGCVGCVGCVGCFSCSNCWNWWFI"
     misc_feature    5290..5305
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 26.71"
                     /tool="antismash"
     gene            complement(5476..5922)
                     /locus_tag="EXW43_RS13665"
                     /old_locus_tag="EXW43_14015"
     CDS             complement(5476..5922)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="EXW43_RS13665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_14015"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_000836819.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAIMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELREELGVTYSIKQFLGVIENQWQAKETLHHEINHIFEIDSNELKSDFTPISKESH
                     LAFHWIDFNKENINSYTIMPTPSVKELLEKKLSDELLTCWISNF"
     gene            complement(6294..6863)
                     /locus_tag="EXW43_RS13670"
                     /old_locus_tag="EXW43_14020"
     CDS             complement(6294..6863)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002065694.1"
                     /locus_tag="EXW43_RS13670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_14020"
                     /product="DUF4256 domain-containing protein"
                     /protein_id="WP_000665071.1"
                     /transl_table=11
                     /translation="MKESNKKELSLEQREELLQTLKDRFENNMNRHKGLEWANVQERLD
                     ANTEKLWSLNEMERTGGEPDVVDFGKEKGEYIFYDCSAESPKGRRSVCYDLEALESRKK
                     HKPENNAIDMATAMGIELLTEEEYRTLHNLENFDMKTSSWVQTPSDIRELGGALFCDYR
                     FGHVFVYHNGAESYYAARGFRGSLRV"
     gene            complement(7047..7511)
                     /locus_tag="EXW43_RS13675"
                     /old_locus_tag="EXW43_14025"
     CDS             complement(7047..7511)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136577.1"
                     /locus_tag="EXW43_RS13675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_14025"
                     /product="N-acetyltransferase"
                     /protein_id="WP_002168287.1"
                     /transl_table=11
                     /translation="MNVQLKIVTRENWEDALKLRVKENQIHLVPAVAVSLAKVYIKPDG
                     ENVEYIPFAIYDGDLIVGFIMHAFVRESTDMYWINGFIIDEKEQGKGYGKAALQEIINL
                     IKNNFKECEEIRLTVHKDNISAKKLYECYDFKPLGNVYDGEEVYRLLLLK"
     gene            complement(7589..8512)
                     /locus_tag="EXW43_RS13680"
                     /old_locus_tag="EXW43_14030"
     CDS             complement(7589..8512)
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="EXW43_RS13680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_14030"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_002127616.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATGPLTNEITTYYPEIHGPEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGEDLTIVDVGRSTSLAIAFNLWNELMLNIKGIYFMGGVFLEAGNVTPLA
                     EANVYGDPIASQIVFQQAKNITLFPLNVTNKTVLTPSVFTYILVNTQNPFKSIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVLAISGLVNPTFLNYTSRKVTVDICGETKGQTFADFRPH
                     SKSEGARIALQLDEEKFIQDFMKIML"
     misc_feature    8762..8778
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.88"
                     /tool="antismash"
     gene            complement(8796..9707)
                     /locus_tag="EXW43_RS13685"
                     /old_locus_tag="EXW43_14035"
     CDS             complement(8796..9707)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136580.1"
                     /locus_tag="EXW43_RS13685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_14035"
                     /product="DMT family transporter"
                     /protein_id="WP_078203292.1"
                     /transl_table=11
                     /translation="MHNKKWDFRIVCAHAFTILIWGTAFPGIRMGLEAYTPEHLTLLRL
                     LIASLILFLFSLIYKLRLPDLKDIPVIFIFGALGFTIYHIALNYGEKSVNAGPASLIVS
                     VTPILTAILASIFLNERMKLNGWIGGVISFVGIAFLSLSQGDSIQLNSGGLLILLAAIS
                     ESLFFVFQRPYLKKYGFLPFTIYTIWSGTVFMLIFLPGIYQEIVTAPIEITLSVIYLGV
                     FPTVLPYIALAYITSHASASEATSSLYLTPVTACIIAWIWLGEVPTFVSIIGGGITILG
                     VVIAHLTFNKDDYKQIESEKVW"
ORIGIN
        1 tgttgtgtct cggatatttt accaatttga gaagcgttgg aagaaatttg tattgtgcat
       61 tcgtcgatga tttctaagtt aacgttgttg tttgcccatt cactagccaa taaacttgac
      121 cgatctacac cttgtaaaat cttttttcta tctatattaa taatcgtttg ggattcattt
      181 ggaattaggc cggatatatt aggatatttc ccttcaataa gccttgaata caacgtaatt
      241 gtaccgaatt taaaaataat gtgactctct gaaagatata tgtatactaa atttgaatta
      301 ctgctcatta attttagtag ttcactaata gtggaacttg gtacaatgca atttaatttc
      361 gcatgtgaag aaatgggtat ttcacgtaaa gccagtctgt gcgaatcagt agcagcacaa
      421 attaatttgt tatgatcaat aacgaaatgt acgccagtaa gaactggtct agattcattt
      481 ttagcagttg caaagacagt ttgtttgaat acctcaatca gctgttccgt ttctacttgt
      541 atttctgtat gtgagtctaa aaatggtaca ttaggaaatt catttgccga gagtccattt
      601 aagtttaaag taatttcatc tgattgaatt gtaatcaact gctcattcat actttttatt
      661 aaaatctcac ttggcatttt cttaataatt tcaatgaaat actttgctgg tacaacaata
      721 cttcctgctt ttaaaatagt tgtaatttgt tcgtcttcaa ttgaaatagg tataaatttt
      781 tcaatgaaaa tattcgaatt acttgcgatt aaagtaattc cagattgatc tgctgttatc
      841 tttattccgg ataatatagg aattaaagtt tttgttgaga tagctttact tacttctgaa
      901 agagcttgtg taaattgttt gtgattaacg ataaactcca tttttccccc gccttaaatg
      961 aaatactatc tttatgaaga ttgatgaata atagttccat taaaaatata aggaactgtt
     1021 tctttctttt gtaaaaggct ttttaagtgt tctggtatac gtaaataagg ttctgctgaa
     1081 tctaattcca tccaggtaat ctcctcaatc tcttttggcc gtgatataca gatttcccct
     1141 ccggttattt ctcctaaaaa attaaagaag attgcatgat gtcctctctc ctcaaaaaag
     1201 gcttcactaa tggaacaaac cccttttacg gatatgtcta gccctgtttc ttctttcact
     1261 tcacgaattg ctgcctcttc taacgtttcc ccgaatttaa ctgctccacc tggtaaagtg
     1321 taataagaac cattttcccc tttgttttta accattaata gcttttcatg agtttcatca
     1381 tatagaagtg cgtatgtaac atttactttt ttcatatgta acccccttga atggtgaaaa
     1441 ttttctgtct ttattttatt ctatcattaa attgttttga ttcataaaaa gtaatttgag
     1501 taaataacta atacgaaacg atatcgtgtt catagacaca caaaagaaaa gagcatattt
     1561 tgaaaaagat taatcataat acactcttta atgattcatt tataaccttt tttctaaata
     1621 gtaatgatta tgccctttag gataatcttc acttacccct atcactttat atccatgctt
     1681 cttataaaaa ttaggggctt ggaaactaaa agtatctaat ttgattagcc tacattcttt
     1741 ttttattgca aattcttcaa taagattcat taaattgctt ccataacctt catgcctgta
     1801 ttcttcagaa acccatagga agtcaacata tacatggtgc caaaatgtga ttgccgttat
     1861 ccctcctact atttcttctt tttcattctt cactataaaa ctaatctttt ctgaaggtgt
     1921 tttttcttca tttgaaagtt gtttttggtt atattcaatt actttttctc ttatgtaatc
     1981 actatcttct tgtacccatt gctttactat ttgcatttta actatcacgc ccctttacat
     2041 tatttattca atttaataat cggaatttcc tatcttcttt aatgttagat tatgaaataa
     2101 gtaaagggaa aaaattagat tttttgaatg tcattcattg tttatacatt tttttgtaaa
     2161 gcaaaagagc cctagtataa ggactctttc ttagcattaa gctatttttc gatttaacga
     2221 tttgatttcc ttcttctttt ctgcatttgg agggcggatt actatagaga taacgaaaga
     2281 tataacacat agaacaccga ttgtaatgaa ggttggctga aatccgccaa gtagtgctcc
     2341 aatgaatgat cctgcaagtg caccaaatcc gaaaccttgg taaacaatcc cgtagtttgt
     2401 actatgattt tttaatccga agaagtctcc cacaattgct gggaagatag tgatgttacc
     2461 accaaagcaa aaggcaacac ttgctacaca agcaaaataa attccgtaat ttagtggaat
     2521 aaaacttaaa gtgaagacgg ataatccaat aataataaat gtagccgaga cgatttttaa
     2581 tcgacctatt ttatctgata acgttccaag aataattcta cctattgtat taaagattgc
     2641 aatcattgcg acggcattag cggccgtcgc tgcactaaga ccaacgagct gcaccccgat
     2701 gtcttttacc ataccgatta aatacagtcc gcccatacat gatgtgaata acatgaaaaa
     2761 taagagatat acttgtttcg ttttcatcat ttcacgcggc gtataatcat tgtgtaatgt
     2821 ttcagttgca gtgttactta caattgcttc acgtaagaag aatgatccta tcaacactaa
     2881 tagtaagacg ataatacccc aatataaaaa tgcttgtgat actccaagac tgttgataag
     2941 atttccgttt acatatctaa agattaagct tcccattcca tatgccgaaa cagatatacc
     3001 agaaataagt cctttacggt ttggaaacca tttaattaaa ttagatagtg atgtaatata
     3061 tgctgttcca tcagcgtatc caacgacaac accggctaat aaataaagta atggtaatga
     3121 ggaaacttgt gaactaagta ttaaaccgag tcctaaaaca attcctgcag tagcgataag
     3181 tttacgaagt ccaaattttt gttgtagctt tcctgcaaat agagttgaga atgataaaga
     3241 aaaactcgta attgagaacg tgatcgcgac tgaattgagg ttccatccaa acttacttac
     3301 aaggggttga ttaaataaac tccatgtgta aattgttcct aggccaattt gaacaatgat
     3361 tgtacctaga acaattagca atggatttat agatgtttgt ttcatgctaa ctgtcccctt
     3421 cttttatcat gacatgaatt ttcacagtta gtatatccat gataaattgc aagatactgt
     3481 acggaaaatg gaaattgagt tagcttacac aaaaagacac ttcatcaaga agtgcctttt
     3541 tgtaggttat atacagcatt taaagaaagc tttgttaaga taagacattt tcgaatgctt
     3601 atgtatatat gttatcaaat aatttgctga aagtgatgag acttaagacg tatttttctt
     3661 tcataaataa gtcggagaga aaattaatat tcaatttcct caaagtcttt cggctgcggt
     3721 acagtttgca tattagctgc atctttcgga tcgctataaa tcgcgttttg ttctgtgttt
     3781 acatccttta tattttgctt tttattttta ttcacttcat ttttcattaa agttcactcc
     3841 tttttattat aggagtagtt ataccctaac catagtattt ttactgctac taaatgtatc
     3901 agcgactaaa ttgctaaatt cacgtaaata atataaataa ttatccccac catagttgta
     3961 tcttttattg tacattttag caacgacgac attatattct ggaatgacta atattgttgg
     4021 tcctgtaatt cctaatattt gataggaacc tttaggaact cgctcaccaa gttcgctaaa
     4081 taaagcaggt tcattttgaa cgaaccaaaa caacccgttt tgaggtagtt ctttatttgt
     4141 atactctgga ctttgtaagc tcgtagcaag tgctataact tcttttggca caatttgttt
     4201 accatttaaa aaccctttat ttaaatgaag gttaccccaa aatgcaaatt ctctcgcaga
     4261 gacaaaaaga tttttctcgg agccgtcatc tactgcacca acactactta ttgcatcttc
     4321 atctgggttt acaacaacct taactaaatt atcatcttgt tttattctcc atccagtttt
     4381 tttgaaatca gcaggtgtga atacacgctc ctttaatagt tcaggaaaac tcttattata
     4441 cagttgataa ataagctgcg tcaccattct tacattaata tctctgtacg cccaagcttg
     4501 tcccggctca aattcacgaa aagctgtccc atcatcggtt tcatttaaac cgtgtgaatg
     4561 agttactaaa tgtcttatcg ttgtctttcc aagtaatgaa ggatcatatt ctttaaaata
     4621 atttacagcc tcgtcatcaa gagattttat tatcccctca taaactgcat aagctaccat
     4681 taatcctaaa tagctttttc ttgcggaagc gacattgaat tgtgaagata tagttacttt
     4741 tttactagta ggagtatttg aatgcgttcc actataatgc tctagtacaa tcttattatc
     4801 tttaataata caaagtgctg ccgcagaact ttgattggtc tctttaatgc tttctaccca
     4861 tgaaattaat ttttcatatt tgtacaaata aatatccccc taaaacatat aaaaataaca
     4921 gcatgaaaga aaagtttcaa tacattataa ctttgcatct gcacttttct ttcatgtgaa
     4981 atattataga tctataatac ttaaataaac caccagttcc agcaattaga acagctgaaa
     5041 caaccaacac agcctacaca acctacgcaa ccgccacaac gtccgccgcc acaaccacca
     5101 cagcgaccac cacaaccgcc acaacgtccg ccgccaccgc atccgccaca acgacgtgca
     5161 tcgtcttgaa tgtagtaata tggatattga ttttgttgct gatcccaata cacaacatta
     5221 ccaggttgat aagcattaag gtttaacgct tgtagttctt gttgaaactc attcattatg
     5281 aaaccctcca tttacaaatg taaattccgt ccaaattttc gagtttattt acttttaaaa
     5341 ataaagtaga aataaggata tagactaaat gcgtacatca acaaaatatg actttacact
     5401 aggtgatgta ccttgtttat gagcctattt tctttatatt cttttagtgg acggaattaa
     5461 tataaataga gacaattaaa aattactaat ccagcaagtt aatagttcat cacttaattt
     5521 tttttctagt aattctttta ctgaaggtgt cggcattatt gtataggaat ttatattttc
     5581 tttattaaaa tcaatccagt gaaatgctaa atgggactct ttagatattg gagtgaaatc
     5641 ggattttaac tcatttgaat ctatttcaaa aatgtggtta atttcatggt ggagggtttc
     5701 tttggcttgc cattgatttt ctatgacgcc taaaaattgt tttatactgt aagttactcc
     5761 aagttcttct cgtaactctc ttattagtgc attctctgct gattccccaa tttcaacatg
     5821 accaccgggt aaaaaatagt gatggcctat gtattcagca actagtaatt tttcatcttt
     5881 tatcatgata gctcgtacaa tatgatggaa tttacttttc attttgtatt cctcctactc
     5941 attcacttta tacttgctcg aaaattttca tttaaaagcg ggataatata aatcataggt
     6001 gtttcatatg gtgaatttca ttgataattg caagagtacc ttttacaagt tctcgtttat
     6061 acgttaatta aaactaataa ttttgataaa tgattctaaa aaagagtatg tttatgaaga
     6121 aaattatcaa aacatactct taatattttt agttgattca ttcttttaca ataaggtttg
     6181 attagcccta ttctttgcgg cctcaaaatc gaagccgtag ctaaagtatg caatcactac
     6241 tattccctaa gcaaatctat tctctacttc aaatttacct gcctgtacaa attttagact
     6301 cttaacgaac cacgaaaacc tcttgcggca tagtaggatt ctgctccatt gtgatacaca
     6361 aagacatgtc caaagcgata atcgcaaaaa agggcaccgc caagttctct aatatcagaa
     6421 ggtgtttgca cccaactcga cgtcttcata tcgaaatttt caagattatg taatgtacga
     6481 tattcttctt ccgttaatag ttcaataccc atggcagttg ccatatcaat agcgttattt
     6541 tctggtttat gtttctttct tgattctagc gcttcaagat cgtaacaaac acttctacga
     6601 cctttaggac tttctgctga acaatcataa aaaatatatt cgcctttttc tttaccaaaa
     6661 tcaacaacat caggttcgcc accagttcgt tccatttcgt tgagcgacca caacttttca
     6721 gtattagcat ccagcctttc ttgaacatta gcccattcta aacctttatg acggttcata
     6781 ttattctcaa aacgatcttt caatgtttgt agtaattctt cacgttgctc tagtgacaac
     6841 tcttttttat tgctttcttt catattagtt cctccattga tgtttttatt aatgatagtt
     6901 taagtatata taaatactgt tttataataa aatgtttaaa taattgcgtg aattgagttt
     6961 gcgataattt atatgaatta gtacaaccaa ttctccgcaa tatgagcctt taacggaaca
     7021 acttattgtt tcaaaacagt acatctttat ttcaacaaca ataagcgata tacttcctca
     7081 ccgtcatata catttcctaa tggtttaaaa tcataacatt catatagttt ctttgcagaa
     7141 atattatctt tatggacagt taatctaatt tcctcacact ctttaaagtt attttttatt
     7201 aagttaatga tttcctgtaa tgctgctttt ccatatcctt ttccttgctc tttttcatca
     7261 atgataaatc cgttaatcca atacatatct gttgactctc gtacaaaagc atgcataata
     7321 aaaccaacta taaggtcacc atcatatata gcgaatggaa tatattcgac attttcacca
     7381 tctggtttta tgtatacttt agcaagtgat actgccactg cgggaacaag atggatttga
     7441 ttttctttaa ctcgtaactt taatgcgtct tcccaatttt ctctcgtaac aatttttaat
     7501 tgaacgttca ctattttaag ctcctttaaa aagagaattt atcttttata ttcaacggag
     7561 tgaaatgatt ccctttttcg acatcaactt ataacatgat tttcataaaa tcctgaataa
     7621 atttttcctc atctaattgt aacgctatac gcgccccttc agatttagag tgtgggcgaa
     7681 aatcagcaaa agtttgtccc ttcgtttcgc cacatatatc cacagtcact tttcgggatg
     7741 tataatttaa aaatgtagga ttcactaaac cgcttatagc aagtacatcg tgtaataatg
     7801 gcccttcaat agatggattg agtttttggt atgctgataa ataataatcg tacatgggtt
     7861 tcataatcga cttaaatgga ttttgtgtat taactaaaat gtaagtaaat acacttgggg
     7921 ttaaaaccgt tttatttgtt acatttaaag ggaataacgt tatatttttc gcttgctgaa
     7981 atacaatttg acttgctata ggatctccgt acacgtttgc ctctgctaat ggagtaacat
     8041 tccctgcttc taaaaagaca ccacccataa aataaattcc ttttatattt aacatcaatt
     8101 cgttccataa attaaaagca atcgctaacg aggtagatct tcctacatcg acaattgtta
     8161 aatcttctcc atactttaca agaatcgcag caatactacc gaaattgtat ataggaatag
     8221 ataacacact ttccggtaca tggatgggcc ctaatccttc tggtccatgg atttcagggt
     8281 aatacgttgt aatttcattt gtaagtgggc ctgttgcacc gctaattaca ggaatatctt
     8341 gtctgtttgc taactgtaaa atatatgcgg cattatgagc ggcatgtata tgttcaacat
     8401 ttccatatcc agttacgata ccaacaattt caatttcagg atgcagtaag ccatacataa
     8461 ttgcaaacga atcatcaata cctgggtctc caaaaaataa aaccttctcc aagaaatgca
     8521 ctcctttcat aggtaagata attatgtata tgtggaaaaa aattaaatct atacagttct
     8581 aagcaaatat taatggtgag taatgtattg ataaaaaaga actagcatta tgctagttct
     8641 tttgcagata aataatctac cagtttaatt aaatctcgta caaatattgg actaactgat
     8701 tttaatatag atttttttca ctatatttat agaattgaaa acctgttatt aatccgaaat
     8761 tttgcattca aattgcaaat actcgtaggg atgtcttacc ataccttttc actctcaatt
     8821 tgcttataat catctttatt aaatgttaaa tgagcaatca caactccaag tatagtaatt
     8881 ccccctccaa ttatcgaaac aaaggttggc acctcaccta accatatcca agcgataata
     8941 catgcagtta ctggtgttaa atatagagaa cttgttgcct cagaagcact ggcatgggat
     9001 gtaatatatg ctaatgcaat atacggcagt actgtcggga acacacctaa atatataaca
     9061 ctcaaagtaa tttctatagg agctgttacg atttcttgat aaatccctgg caaaaaaata
     9121 agcataaata cagtaccaga ccaaattgta tatattgtaa atggcaagaa accgtacttt
     9181 tttaagtatg gcctttgaaa aacgaaaaat agactttctg aaattgccgc caataatatt
     9241 agtaatcccc cactattcaa ttgaatggaa tctccttgac ttaacgataa aaaagcaatt
     9301 cctacaaaac taattacacc accgatccaa ccatttaatt tcattctttc attcaaaaaa
     9361 atagaagcaa gaattgccgt taatataggt gttactgata caattaaact agcaggtcca
     9421 gcattcacac tcttttcacc gtagttcagt gcaatatgat aaattgtaaa tccaagagca
     9481 ccaaatataa aaattactgg aatatctttc aaatcaggta aacgtaactt gtatataagt
     9541 gaaaacagaa aaagaatcaa agaagcgatt aataatcgta gcaaagtaag gtgttcaggg
     9601 gtgtaagctt caagccccat ccgaattcct ggaaaagctg ttccccatat aagaatcgta
     9661 aaagcgtgag cacatacaat acgaaaatcc cattttttat tatgcatata ttctctccct
     9721 ctccaataaa ccagccattt tctaattacc aattatttct tttcatcata tttaatagtt
     9781 gatacaatgt cttcaaccag ttaatcctcg aaaaaaccaa ccactttgca tttttaaaat
     9841 gaatgaggga gtttttaata tgtctactga taaaaaactg cctaaatatc ggcagataat
     9901 agactatatg aaagaaaaaa ttgaatacgg agaatggccg attggaagta aggttcctag
     9961 ccagcggcag ttagcgaaat tatttcatgt aaatcgtagt actgtaataa ttgcactaga
    10021 agaacttatg gcagatggct taattcaagg gaaaatcggg tcaggaacaa ttgtaacgaa
    10081 taatacatgg tcattattag ccaccaatcc cccgcccgat tggagtgaat atgtaaaggc
    10141 tggtattcat aaaccaagca agttaatggt acgagaaata aatgaagcag aagcaaataa
    10201 aaaattgatt catcttagta aaggagaact tgcacctcat cttttcccgt tagaaactat
    10261 gcaattcatt atgcgt
//
