LOCUS       NZ_CP036117            10330 bp    DNA     circular CON 12-DEC-2024
DEFINITION  Bacillus mycoides strain JAS481 chromosome, complete genome.
ACCESSION   NZ_CP036117
VERSION     NZ_CP036117.1
KEYWORDS    RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus; Bacillus cereus group.
REFERENCE   1  (bases 1 to 5296574)
  AUTHORS   Fiedoruk,K., Drewnowska,J.M. and Swiecicka,I.
  TITLE     The dynamics of pangenome and proteome of selected Bacillus cereus
            sensu lato representatives from the primary and anthropologically
            changes environments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5296574)
  AUTHORS   Drewnowska,J.M., Fiedoruk,K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2019) Department of Microbiology, University of
            Bialystok, 1J Ciolkowskiego, Bialystok 15-245, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 187.86x
            Sequencing Technology :: Illumina MiSeq and MiniIon Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018741645.1-RS_2024_12_12
            Annotation Date                   :: 12/12/2024 04:46:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,189
            CDSs (total)                      :: 6,036
            Genes (coding)                    :: 5,734
            CDSs (with protein)               :: 5,734
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 302
            CDSs (without protein)            :: 302
            Pseudo Genes (ambiguous residues) :: 0 of 302
            Pseudo Genes (frameshifted)       :: 122 of 302
            Pseudo Genes (incomplete)         :: 192 of 302
            Pseudo Genes (internal stop)      :: 81 of 302
            Pseudo Genes (multiple problems)  :: 78 of 302
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:51:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2295853
            Orig. end                         :: 2306183
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036117.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10330
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10330
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10330
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5330](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5330
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(70..86)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.99"
                     /tool="antismash"
     gene            761..1366
                     /locus_tag="EXW43_RS12235"
                     /old_locus_tag="EXW43_12520"
     CDS             761..1366
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853587.1"
                     /locus_tag="EXW43_RS12235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_12520"
                     /product="PRK06770 family protein"
                     /protein_id="WP_002127350.1"
                     /transl_table=11
                     /translation="MKTLFKIIGIIAGMAVIGVGVTYGMLYYLNNSKPAAKKASPAAPA
                     VEVLADSNVKAEDAKILENGNYSLPNSGFNKNFKWTDENIQTALHEMAHQKAKADQKWG
                     YIFITQERIESLLEIINSNDVAQKSTYVDILERWKQGKYEKVDVDHNKIWKLQSGNLGE
                     GKGVMSEKEQKELINQVFIQKGTYSGSKLIAGEVQANK"
     gene            complement(1472..2344)
                     /locus_tag="EXW43_RS12240"
                     /old_locus_tag="EXW43_12525"
     CDS             complement(1472..2344)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016095293.1"
                     /locus_tag="EXW43_RS12240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_12525"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_215576180.1"
                     /transl_table=11
                     /translation="MEMRHVKTFCAIVKYGSFSKAAHALGYAQSTVTAHIKALENDLNI
                     PLFDRLGKKILLTKAGHQFHPYALELLTIYDKAQEIPQNSDHLEGTLAITSNESLAVYR
                     LPQLLRTYKQENPKVNIVLETNTNEQALKKLHEGETDVIFIIGESIEHNDFITCNLCNE
                     TFGWIFPAHFPSNTDSLLLLQDTQFIFTEQSCGYRPMVDRFLRQSGRNPNKTFETSNVE
                     VIKQSVMCELGISILPYIVVQESNQKEQLCFQPIETSAVIQSHVIYHKSRWISPVLQSF
                     LTLLERIEK"
     gene            2474..3517
                     /locus_tag="EXW43_RS12245"
                     /old_locus_tag="EXW43_12530"
     CDS             2474..3517
                     /EC_number="1.2.1.11"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1219: N-acetyl-gamma-glutamyl-phosphate reductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200507.1"
                     /locus_tag="EXW43_RS12245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_12530"
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="WP_002168402.1"
                     /transl_table=11
                     /translation="MSEKSYHVAVVGATGAVGQKIIALLERVTKFNIEEITLLSSKRSA
                     GKIVQFKGREIKIQEAKSTSFEGVDIAFFSAGGEVSRQYVNHAVSSGAIVIDNTSEYRM
                     AHDVPLVVPEVNAHTLREHKRIIAVPNCSALQMVTALQPIRKSFGLERIIVSTYQAVSG
                     SGIHAIHELKEQAKSMLTGEEVKSTILPVKKDKKHYPIAFNVLPQVDIFTDNDFTFEEV
                     KMIQETKKILEDPNLKMAATCVRVPVVSGHSESVYIELEKEATVAEIKEVLLNAPGVIL
                     QDNPSEQIYPTPLYAEGKRDTFVGRIRKDPDTSKGFHLWIVSDNLLKGAAWNSVQIAET
                     LVENGII"
     gene            complement(3764..4057)
                     /locus_tag="EXW43_RS12250"
                     /old_locus_tag="EXW43_12535"
     CDS             complement(3764..4057)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002168401.1"
                     /locus_tag="EXW43_RS12250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_12535"
                     /product="hypothetical protein"
                     /protein_id="WP_033733961.1"
                     /transl_table=11
                     /translation="MCNSKPIDELTIEDLKQNPIWEWAIDEAENEECDETWVKPVEIIN
                     FTEELNGSIVLGELIIHNDEKFPMMCSIDIENNEVLISSIVFITKKKMSILL"
     misc_feature    complement(4288..4305)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.83"
                     /tool="antismash"
     gene            5001..5330
                     /locus_tag="EXW43_RS12255"
                     /old_locus_tag="EXW43_12540"
     CDS             5001..5330
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002189588.1"
                     /locus_tag="EXW43_RS12255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_12540"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_215576181.1"
                     /sec_met_domain="TIGR03601 (E-value: 3.6e-28, bitscore:
                     96.1, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNFQQELQALSLNDYQPGNIVYWDQQNQYPYYYITDDARRCGGC
                     GGGRCGGCGGCGRCGGGRCGGFRCFGCFGCFSCFGCGGCSNCSNCSNCSNCFDGFNGTT
                     VTFEY"
     gene            complement(5639..6541)
                     /locus_tag="EXW43_RS12260"
                     /old_locus_tag="EXW43_12545"
     CDS             complement(5639..6541)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003203974.1"
                     /locus_tag="EXW43_RS12260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_12545"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_215576231.1"
                     /transl_table=11
                     /translation="MISIELRQLEYFLAVSKELHFTKAAEKLNISQPSLSQQIRALEHE
                     VGMPLFDRIGKKISLTEAGRVLLSHCKTIFHEVEQARSAIQDLNGLHHGSLTIGSLLTV
                     VNYLLPPAILDFNKQYPNIELSVLGLRTGEIREKLLQNELDMGITFLPVQDKEIVSIPL
                     YKSELTLVVPTSHSLTERKYVSIEELQDYPLILLPKNFYLTELITSHCQNFNFKPKPIL
                     EISTMESLIQMVAQGMGITVLPKPYIDFLQNEHIQAIKIKNPTPTIDIGLIYRRDKYMC
                     AATRKFIEQLKITVHSLQK"
     gene            6671..7996
                     /gene="gabT"
                     /locus_tag="EXW43_RS12265"
                     /old_locus_tag="EXW43_12550"
     CDS             6671..7996
                     /EC_number="2.6.1.19"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (34-374). E-value: 4.7e-122.
                     Score: 399.2. Matches aSDomain:
                     nrpspksdomains_EXW43_RS12265_Aminotran_3.1"
                     /codon_start=1
                     /gene="gabT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200502.1"
                     /locus_tag="EXW43_RS12265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_12550"
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_002031963.1"
                     /sec_met_domain="Aminotran_3 (E-value: 9.8e-132, bitscore:
                     437.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKSSVLPGSYSQVLQKRYEEAVPTGIYHLTPLYVKEARGAIITD
                     VDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAESFIHPCFHVTPYENYITLAEKINEK
                     VPGTSKKKTMFANSGAEAIENAVKIARKVTGRSKVISFERGFHGRTLMTMSLSTKETPY
                     KAGFGPFVSDMHILPYPYYYRAEEGVSPEEVDSQVIYQFEKFLEEVSPEKVAAIVMEPV
                     QGEGGFIVPSISFVKRLREICNQYGILMIMDEVQTGFGRTGKWFATEHFHIEPDIMTLS
                     KSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGSPLSCAAGLAVFEIMEEENLVERAQQ
                     IGNRIMSHLHQMQLKYEMIGDVRGIGAMVAMELVTNRITKEPAKELTVKLIEDFWKNGL
                     ISIGAGIFDNVLRFLPPLVISNEEIDRGFEIINESFEKLCRK"
     aSDomain        6773..7792
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EXW43_RS12265_Aminotran_3.1"
                     /evalue="4.70E-122"
                     /label="EXW43_RS12265_Aminotran_3.1"
                     /locus_tag="EXW43_RS12265"
                     /protein_end="374"
                     /protein_start="34"
                     /score="399.2"
                     /tool="antismash"
                     /translation="VKEARGAIITDVDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAE
                     SFIHPCFHVTPYENYITLAEKINEKVPGTSKKKTMFANSGAEAIENAVKIARKVTGRSK
                     VISFERGFHGRTLMTMSLSTKETPYKAGFGPFVSDMHILPYPYYYRAEEGVSPEEVDSQ
                     VIYQFEKFLEEVSPEKVAAIVMEPVQGEGGFIVPSISFVKRLREICNQYGILMIMDEVQ
                     TGFGRTGKWFATEHFHIEPDIMTLSKSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGS
                     PLSCAAGLAVFEIMEEENLVERAQQIGNRIMSHLHQMQLKYEMIGDVRGIGAMVAMELV
                     "
     gene            8011..8700
                     /locus_tag="EXW43_RS12270"
                     /old_locus_tag="EXW43_12555"
     CDS             8011..8700
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002031964.1"
                     /locus_tag="EXW43_RS12270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_12555"
                     /product="MOSC domain-containing protein"
                     /protein_id="WP_002168398.1"
                     /transl_table=11
                     /translation="MTKVYEILSLNLGLPKEVMYGGKLIQTGINKKQVKEPVFLSFLKF
                     NGDGQADLVHHGGVDKAVCVYTGDHYPYWEKELNQELVYGAFGENITVSGMREEDVCIG
                     DTFQLGEAIVQVTQPRQPCFKLAKKYNIPKLPLYFQDTGYTGFYFRVLKEGWILPVDTL
                     KLLKLDPKGVSILFANRIMHKEKQNSEGLKRILEVNALSTSWRKTFEKRVKGEEISTKE
                     RLEGTKE"
     gene            8963..9847
                     /locus_tag="EXW43_RS12275"
                     /old_locus_tag="EXW43_12560"
     CDS             8963..9847
                     /EC_number="2.3.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526760.1"
                     /locus_tag="EXW43_RS12275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW43_12560"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_003189191.1"
                     /transl_table=11
                     /translation="MGNVEKMANFLLEEPRREQLFPLFEEVFGITSQTLQDFSEKGYWD
                     YTYKALSFLQDDNAIANVAAFSLPLLINGEKINATGIQSVMTHPNYRRRGLMKQLFSKI
                     IEEIDEQCECAVLFTENPELYKPFGFQIVQEYLMTVSYENKGYKDSQLKKLDFYNEEDR
                     QLIHEIIENGQRLSNKFSTLNYRSSFYLNMYDSTWNENLYYSEKLDALIVFTVENQKLK
                     LFGVFAPVIPILDELCEEISGEFTEIEFYFYPDQLGIDDVQYTELQTSKYLMVRSNKEL
                     DFKGYKFPVLTEF"
     misc_feature    complement(9931..9947)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.8"
                     /tool="antismash"
ORIGIN
        1 tggtattttg aaaaacaagt agagcgctgg aaaatagcaa tacagaagtc tcagcaatat
       61 gaattagtaa tactcgatgg ggatatatat cagccactta gttataattg gtgttttcgt
      121 tttgatatat ttaatcagcc gttatcttta atagaaaact tttataaaga aaagatgata
      181 aatagagaaa ttggttttcc agatcaatat ttttatttat atacaagtga tgaagagctt
      241 agaaaacgga aagagtttga tgggacgaaa aaaagaagaa actttgaaaa acatttacat
      301 atatctaaag catttcagcg ttattatgaa aatctcaata ccgtcacaga cgggtactgt
      361 aaattgattg aagcaaaaag tataaagtcg aatgaattag aaattgtgaa aagtttaaac
      421 agcttaaaag tatgtgaaga aagacgtttt gatgtttcga ggttagatgc tattacggag
      481 tggttaaaag aaaatcgtgc ataaagtaaa aacaacgagt gttttagaag gcgctcgttg
      541 tttttttgtt tccatataaa tataaaggaa aattttgtag aatatcttta ttttttaaca
      601 gaataatctt tcaatttcac gaaaaaagaa atataatata aggaaatata tataaataat
      661 atgatagggg atgggatgaa ctattttgaa tgaaggaact gttttatata agaaataatg
      721 aggtagtaag aacagagata tttgggagga aacaagcaag atgaaaacat tatttaaaat
      781 cataggaatt atagctggta tggcggtaat cggggtaggt gtaacgtatg gtatgctgta
      841 ttatctgaat aatagtaaac cagctgcgaa aaaggcatca ccagccgctc cggcagtaga
      901 agtgctagct gatagtaatg taaaagccga agatgcaaag atattagaaa atggaaatta
      961 ttcattgccg aatagtggtt ttaataaaaa cttcaaatgg acagatgaga acatacagac
     1021 agcattacat gaaatggcac atcaaaaagc gaaagctgat caaaaatggg gctatatttt
     1081 tattacacaa gaacgtattg aaagtttact tgaaattata aatagtaatg atgttgcaca
     1141 aaaaagtaca tatgtggata ttttagaacg atggaaacaa ggaaaatatg aaaaggttga
     1201 tgtggaccac aacaagattt ggaaattaca aagtgggaat ttaggagaag ggaaaggagt
     1261 aatgtcagaa aaggagcaaa aagaattaat taatcaagtt tttatacaaa aaggtacata
     1321 ttcaggtagt aaattaattg caggtgaggt acaagctaat aaataatata catattgatt
     1381 ttgaaacacc tgtattgtta tgaacacaaa tgttttaaca aaaatgagta attgcttttt
     1441 agagcagtta ctcatttttg taattactcg cttacttttc tattctctct aataatgtaa
     1501 gaaacgattg caaaactggg gatatccatc tagatttatg atagattacg tggctttgaa
     1561 tgacggctga agtttcgatg ggctgaaaac agagttgttc tttttgatta ctttcttgta
     1621 caacgatgta agggagaata gaaattccta actcacacat aacggattgt ttaatgactt
     1681 caacattgga agtctcaaat gttttattag gatttcttcc gctttgacgt aaaaaacgat
     1741 ctaccattgg tctatagcca cagctttgtt ctgtaaatat aaattgagta tcttgtaata
     1801 aaaggagtga atcagtgttt gaagggaaat gagcagggaa aatccatcca aatgtttcat
     1861 tacataaatt gcacgtaata aaatcattat gctctatact ttctccaatt atgaagataa
     1921 catcggtttc tccttcatgt aattttttca atgcttgctc attggtattt gtctcaagta
     1981 cgatatttac tttcggattt tcctgtttgt aagtacgtaa taattgtggt aaccgataca
     2041 ctgccaatga ttcattagat gtaatagcca atgttccttc taaatggtca ctgttttgag
     2101 ggatttcttg cgctttatca taaatagtaa gtaattctaa tgcatacgga tgaaattgat
     2161 gacctgcttt tgtaagaagt attttttttc ctaatcgatc gaatagggga atgtttaaat
     2221 cgttctctaa tgctttaata tgggctgtca cagtagattg cgcataacct aatgcatgag
     2281 cagctttaga aaagctacca tatttaacaa tggcacaaaa tgttttaaca tgtctcattt
     2341 ccataaatga tacctctttt attatcatga tttgtgaatg attatatcat gtatttcaat
     2401 ttttcaaatc attaaatttc ttttaaaatt aagttgtgga gaaattcatt caattgaaat
     2461 tggaggagaa aaaatgagtg aaaagagcta tcatgtagct gtagttggag ctacaggcgc
     2521 agtagggcaa aaaatcattg cattgttaga gagagtaacg aaatttaata tagaagaaat
     2581 tacattactc tcgtcaaaac gatctgctgg taagatagta caatttaaag gacgagagat
     2641 taaaatacaa gaggcaaaat caactagttt tgaaggtgta gacattgcct tttttagcgc
     2701 tgggggagaa gtttctagac aatatgtaaa tcatgcagtt tctagtggtg caatcgtaat
     2761 tgacaacaca agtgaatacc gaatggcaca cgatgtacca ctcgtcgttc cagaagtaaa
     2821 tgcacatact ttaagggaac ataaaaggat cattgcagtt ccgaattgct cagcactaca
     2881 gatggtaaca gctcttcagc caattcgaaa atcatttggt ttagaacgaa ttattgtttc
     2941 aacgtatcaa gccgtatcag gctcaggaat tcatgcgatt catgaattaa aagaacaagc
     3001 taagtcaatg cttacaggtg aagaagtgaa gagcactata ttaccagtga aaaaagataa
     3061 aaaacattat ccaattgcat ttaatgtatt gccgcaagtg gatatattta cagacaatga
     3121 tttcacattc gaagaagtaa agatgattca agagacgaag aaaattttag aagatccaaa
     3181 tctgaaaatg gcagctactt gcgtcagagt tccagttgta tcggggcatt ctgaatcagt
     3241 ttatattgaa cttgaaaaag aagcgaccgt tgcagaaatt aaagaagtac tactgaatgc
     3301 accaggtgtt attttacaag ataatcctag tgaacaaatt tatccaacgc cattatatgc
     3361 agagggtaaa agagatacat ttgtaggtag aatacgtaaa gatcctgata catcaaaagg
     3421 attccatctt tggatcgttt ctgataattt attaaaaggt gctgcttgga actcagttca
     3481 aatcgcagag acattggtgg aaaatgggat tatttagtat ggatttatat aaatattgaa
     3541 aatagaagga tatttgggaa accgttaatg ataatggttt cctgtttttt acttaatgag
     3601 tattttcctc ttacttcaat taaaatttaa tttaagttag tttttatagt gtttttataa
     3661 atatcaactt tatctgcaga gaattttaaa aatctaggct ctccgttaat tgtgagatta
     3721 atattaagtg ataaaggcat ttcaaccttt ttcactacat cttctatagc aatatactca
     3781 tcttcttttt cgttataaaa acaatggaac taattaatac ctcattattt tcaatatcaa
     3841 tactacacat catagggaat ttctcatcat tatgtattat taattcgcca agtactattg
     3901 aaccgtttaa ttcttctgta aaatttatta tttccactgg ttttacccac gtttcatcgc
     3961 actcttcatt ttccgcttca tctattgccc attcccatat tgggttttgt ttcaggtcct
     4021 caatagttaa ttcatcaatt ggtttgctat tacacattaa tatatactcc ttttgccatg
     4081 aaaatggatg agtaaaaaat ttattcatta attgtaaaat gtcgctattt gcaatcctat
     4141 aaatacaagt ttgctcgttt caataaatag ttgatcgcaa ataagaagtt tcgatgtaga
     4201 gtattatcca tttgatatta ttaaatctag ttttgtaatg ttgaacttat ttttacaacc
     4261 tattaaaagt ctattcttac agtaatctta acaaggtttt aactagacag taaataatgc
     4321 cttttgaaaa ggaaaaccgt ggttgtgatg ggggaaatag caaaaaacat cgctgatttg
     4381 ccattcataa aaatagactt ttcttagcag aaatcactaa ttataaatac ttataaaatt
     4441 agctctgata tagcagatat tgaagcgaag aaaaataaag atgggagtgc atattggaaa
     4501 tacgaagatg accaaacaaa attatggaat atacgactat agaactagaa aatgatgaca
     4561 taaatctaaa tattattgtt taaattcata ttttataatg tccatttagc ccgcattaac
     4621 tgcccgtaaa ccccctctga ttaaagtttc actttataaa aatgagccca tagtaaaaat
     4681 aacctaaaaa taggcatatg aacaaggtgc atcatctagt gataggtcat attttgttgg
     4741 tgtacgtatt gaacttagtt tatcccgcta tttgtgggca gtaaaactcc cacctcaaaa
     4801 ttcaactgga gcaaagaagt taggtgggag ccctgctgcc cgtaaacgcc cgattggtgc
     4861 gggctaataa tcagtggggg atgaacaaaa cccctactga ttaaagtttc actttatagc
     4921 gctatttcta cttagattta acgaacagaa atataggaaa gtatcgatgg ggttttagtt
     4981 tataaacgga ggtcatgaag atgaataatt ttcaacaaga actacaagcg ttaagcctta
     5041 atgattatca gcctggaaat attgtgtatt gggaccagca aaaccaatat ccatattact
     5101 atattacaga tgatgctcgt cgctgtggcg gctgtggcgg tggtcgttgt ggtggttgtg
     5161 gtggttgtgg tcgttgtggc ggtggtcgtt gtggtggatt ccgttgtttt ggttgcttcg
     5221 gttgctttag ttgttttggt tgtggtggtt gctctaattg ctctaattgc tctaattgct
     5281 ctaattgttt tgatggtttt aatggtacta ctgtaacatt tgaatattga taagagacaa
     5341 aaaggaatcc attttgaaaa aagattgatc gaaacggatt cctactttgt gaagcattat
     5401 ataattttta taaaaatgtg ctaacaagtt tgcaaacgga cttgctaaca cctatacgaa
     5461 atagacataa aatatcgctg atgtgtagct taataatacg ataataattt agcacttata
     5521 atgaataata ggttattaga aaagaaacca taaaatgtaa tgttttatat cattataaaa
     5581 agagattatt ctaataggtc gttgtgtaac ggctgcatag gataatctct ttttatgctt
     5641 atttttgtaa agaatgtact gttattttga gttgctcaat aaatttccgg gtagcagcgc
     5701 acatatattt atctcttcta taaataagtc ctatatctat tgttggagtg gggtttttaa
     5761 ttttaatagc ttgtatgtgc tcattttgca aaaagtctat atacggtttt ggtaacacgg
     5821 taatccccat accttgtgcg accatttgaa ttaaagattc cattgtacta atttctaaaa
     5881 ttggctttgg tttaaagtta aagttttggc aatgagatgt aattagttct gttaaataga
     5941 aatttttagg tagaagaatt aaaggatagt cctgtaattc ttcaatagat acatacttac
     6001 gttcagttaa agaatggctg gttggcacaa ctaatgtaag ttcactttta tatagtggga
     6061 tagaaacaat ctctttatct tgcaccggga gaaatgtaat acccatatct aattcatttt
     6121 gtaataattt ttcacgaatt tctcctgtac gaagccccaa cacagagagt tcgatatttg
     6181 gatattgctt attgaaatct aatatagctg gtggtagtaa ataatttact acggttaaca
     6241 aagatcctat tgttaaggaa ccatgatgta atccatttaa atcttgaatg gcagaacgag
     6301 cttgttcgac ttcgtgaaag attgttttac agtgcgataa taaaactctt cctgcttcag
     6361 ttaaagatat ttttttacca attcgatcga aaagtggcat gcctacttca tgttctaatg
     6421 cacgtatttg ttggcttagt gaaggttgtg aaatatttaa tttctcggct gcttttgtga
     6481 aatgtaattc tttcgaaaca gctaaaaagt attctaattg tcgtaattcg attgaaatca
     6541 cctcaagttt aatcatagga tatacctatc attattatat aaataattga attgaacaat
     6601 ggttttgtgg gatatataat cactttatca gaaatggatt gattaaaaaa ttagatgagg
     6661 tgaagaagaa ttgttgaaat ctagtgtatt accgggcagt tattcacaag tacttcaaaa
     6721 aaggtatgag gaggccgtac cgacaggaat atatcattta acgcctttat atgtaaaaga
     6781 agcaagggga gcgataataa cagatgtaga tggaaatcag tttattgatt ttgctggtgg
     6841 aattggtatg caaaatgttg gacattgtca tccgaaagtt gtaagagcgg tacaaaaaca
     6901 agcagaatca tttatacatc cttgttttca tgtaactcca tatgaaaatt atattacttt
     6961 agcggagaaa attaatgaga aagtgccagg tacaagtaag aaaaaaacga tgtttgccaa
     7021 tagtggtgcg gaagcgattg aaaatgctgt aaagatcgca cgtaaagtaa cagggaggag
     7081 taaagtaatt tcgtttgaac gaggatttca tgggagaaca ttaatgacaa tgtctctatc
     7141 gaccaaagaa actccatata aagcaggatt tggtccgttc gtttcagaca tgcatatatt
     7201 gccgtatccg tactattaca gagctgaaga aggagtttct cctgaagaag tggattctca
     7261 agtcatctat cagtttgaaa agtttttaga agaagtatca cctgaaaaag tagcagcgat
     7321 cgtaatggaa ccagtacaag gagagggtgg gtttattgtt ccttccattt catttgtaaa
     7381 acgattacga gaaatttgta atcaatacgg tattttaatg ataatggatg aagtgcaaac
     7441 tggatttggc cgaacaggga aatggtttgc aacggaacat tttcatatag aacctgacat
     7501 aatgactttg tcaaaatcaa ttgctggcgg tttaccatta agtgctattg tcggtcgtgc
     7561 ggagttgatg gacattcctg ggagaggaca aattggtgga acttttgccg ggagtccgtt
     7621 atcatgtgca gccggtcttg ctgtatttga aataatggaa gaagaaaatc tagttgaaag
     7681 ggctcagcaa attggaaata gaataatgtc acatctacat caaatgcaat taaagtacga
     7741 aatgattggt gatgttcgtg gaatcggtgc gatggttgca atggaacttg taacgaatcg
     7801 cataacgaaa gaacctgcaa aagaactaac agtaaagcta attgaagatt tttggaagaa
     7861 tgggcttata agtattggag cggggatatt tgataatgta ctacgctttt taccacctct
     7921 agttatctca aacgaagaaa tagatagagg atttgaaata ataaatgagt cttttgaaaa
     7981 gttgtgccgc aagtaagagg agggatttga atgactaaag tatatgaaat cctatcttta
     8041 aatctaggat tgccaaaaga ggttatgtat ggtgggaagt taattcaaac agggataaat
     8101 aaaaagcaag taaaggaacc tgttttttta tcatttttaa agtttaatgg agatggacag
     8161 gcggatttag ttcatcatgg aggggtagat aaagcggttt gtgtttatac tggtgaccac
     8221 tatccttact gggaaaaaga gttgaatcag gaacttgtat atggggcttt cggtgaaaat
     8281 ataacagtaa gcggaatgcg tgaagaagat gtttgtattg gtgacacgtt tcaactcgga
     8341 gaagcgattg tacaagtaac gcagccaaga caaccttgtt ttaaactagc caagaaatat
     8401 aatattccaa agttgccatt atattttcaa gatacaggat acacaggttt ttattttcga
     8461 gtattaaaag aagggtggat attaccggta gatactttaa aattgttgaa gcttgatcca
     8521 aaaggtgtgt caattttgtt tgcgaatcgc ataatgcata aagaaaaaca aaactctgaa
     8581 ggattaaaaa ggattttaga agtaaatgca ctgtctacta gttggagaaa gacgtttgaa
     8641 aagcgagtga agggagaaga aattagtacg aaggagcgac ttgagggaac caaagaatag
     8701 gtgaattata tttgcataaa atggtgtatt tctattatcg gaaagataag taggaagcac
     8761 catttgttat ataataaata tgatattatt caaaaatctt aatactagtt tattcgactt
     8821 gttttttaat aatcaaaaca taaaataaat taaaccattt ttaaaagttt tatgatacta
     8881 tattgatttg aaaggcctat tttttgagag taggtcatat aagtatatct aaattaatgg
     8941 aatcgacgta tggagggaca acatgggcaa tgttgaaaaa atggcaaact ttctattaga
     9001 agaaccgaga agagaacagt tatttccttt atttgaagaa gtgtttggga ttacgagcca
     9061 aacattgcag gatttttcag agaaagggta ttgggattat acatataaag cattatcatt
     9121 tttacaagat gataatgcga ttgcaaatgt tgcggcattt tcacttccgc ttttaattaa
     9181 tggtgaaaaa ataaatgcaa cgggtattca atcagtaatg acacatccga attatcgtag
     9241 acgaggatta atgaagcaat tgtttagtaa gataatagag gaaattgatg agcaatgcga
     9301 gtgtgcggtc ttgtttactg aaaatccgga actatataaa ccatttggtt ttcaaattgt
     9361 gcaagaatat ttgatgacag tatcatacga aaataaaggc tacaaagatt ctcaacttaa
     9421 aaagttagat ttttataatg aagaggatag acaattaata catgagatta ttgaaaatgg
     9481 ccaaaggctt tctaataaat tttcaacgtt aaattaccgc tcttcgtttt atttaaatat
     9541 gtatgattca acatggaatg aaaaccttta ttattcagag aaattagatg cgctgattgt
     9601 ttttacagta gaaaatcaaa aactaaaatt atttggagta tttgcgccgg taattccgat
     9661 tttagatgaa ttatgtgagg aaatctctgg ggaatttacg gagattgaat tttatttcta
     9721 cccagatcaa ttaggaattg atgatgtaca atatacagaa ttacagacta gtaagtattt
     9781 aatggttcga agtaataaag aattagattt taaaggttat aaattcccgg tattaactga
     9841 gttttaaata ttaatttaaa agctttttta tggaggaatt atgaaaacat catatatacc
     9901 aaatcatatt gagcaactta ctgtaatgaa cgcacctaaa ttttacaaac ttgaagacaa
     9961 tgatacattc attgtccatt cgaaagaaga aacagatttt tggctgaaaa cccattatgg
    10021 ttttgaagta atgaatggcc atgtatttta tgatgagata atgacagatt ttcaggctga
    10081 ggttcaattg cgcatgaatc caaatgcgac atacgatcaa gcgggtttat ttgttatgat
    10141 ttctgagaag tgttggttaa aaacatcttt agaatatatt cctgagggcc catcacattt
    10201 aggtgctgtc gtaacgaata atggctattc tgattggtca acgcaagact atccaaatga
    10261 gtttgctaag caacaattac gttttcgaat tattcgaaaa cttggcgatt atacaatata
    10321 tgtgaaaacg
//
