LOCUS       NZ_CP036070            10264 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Bacillus wiedmannii strain JAS07/5 chromosome, complete genome.
ACCESSION   NZ_CP036070
VERSION     NZ_CP036070.1
KEYWORDS    RefSeq.
SOURCE      Bacillus wiedmannii
  ORGANISM  Bacillus wiedmannii
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5239489)
  AUTHORS   Fiedoruk,K., Drewnowska,J.M. and Swiecicka,I.
  TITLE     The dynamics of pangenome and proteome of selected Bacillus cereus
            sensu lato representatives from the primary and anthropologically
            changes environments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5239489)
  AUTHORS   Drewnowska,J.M., Fiedoruk,K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2019) Department of Microbiology, University of
            Bialystok, 1J Ciolkowskiego, Bialystok 15-245, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.157x
            Sequencing Technology :: Illumina MiSeq and MiniIon Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018741285.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 04:17:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,980
            CDSs (total)                      :: 5,827
            Genes (coding)                    :: 5,631
            CDSs (with protein)               :: 5,631
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 88 of 196
            Pseudo Genes (incomplete)         :: 110 of 196
            Pseudo Genes (internal stop)      :: 59 of 196
            Pseudo Genes (multiple problems)  :: 52 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:47:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2596665
            Orig. end                         :: 2606929
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036070.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10264
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10264
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10264
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5264](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5264
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    304..319
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 20.19"
                     /tool="antismash"
     misc_feature    1228..1237
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 16.22"
                     /tool="antismash"
     gene            1245..2159
                     /locus_tag="EXW41_RS13115"
                     /old_locus_tag="EXW41_13225"
     CDS             1245..2159
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017673335.1"
                     /locus_tag="EXW41_RS13115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_13225"
                     /product="DMT family transporter"
                     /protein_id="WP_088063615.1"
                     /transl_table=11
                     /translation="MNYKKWDFRIICAHAFTILIWGTAFPAIRMGLESYTPEHLTLLRL
                     LIASFILLLFSFIYKLRLPDLKDIPAIFIFGALGFTVYHIALNYGEKTVNAGSASLIVS
                     VTPIVTAIFASVFLNEKMKLNGWIGGVISFIGIAFISFSQGDAIQLNSGGLFILLAAIS
                     ESLFFVFQTSYLRKYGFLPFTIYTILSSTVCMLIFSPGVYTEILAAPLEVNMSVIYLGL
                     FPTVLPYIALAYIISHSGAAEATSSLYLTPVTACFIAWVWLGEVPTFVSIIGGGITILG
                     VLITHISLSKKEKYTTLVNNKSI"
     gene            2282..3643
                     /locus_tag="EXW41_RS13120"
                     /old_locus_tag="EXW41_13230"
     CDS             2282..3643
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080598.1"
                     /locus_tag="EXW41_RS13120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_13230"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_088063616.1"
                     /transl_table=11
                     /translation="MNGKRKIFTCISIIGIGLASFSNSSFAANGTDNSVQTSIPIVNQQ
                     VAAVKEMKPFPQQVNYAGVIKPNHVTQESLNASVRSYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMIITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADSKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGTVNYLKEAQDMITKGIKAS
                     NVTNNNRLNLGDWDSKNSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFSN
                     KYSQNTGLISDFVVKTPPQPAPKDFLDESEYTNAYYYNASRVPLRIVMDYAMYGEKRSK
                     VVSDKVSSWIQNKTNGNPSKIVDGYQLNGSNIGSYPTAVFVSPFIAASTTKSDNQKWVN
                     SGWDWMKNKKESYFSDSYNLLTMLFITGNWWKPIPDDKKIENLINDETQKGYDK"
     gene            3837..4295
                     /locus_tag="EXW41_RS13125"
                     /old_locus_tag="EXW41_13235"
     CDS             3837..4295
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="EXW41_RS13125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_13235"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_215559075.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEDALKLQVKENQTKFVPSVAVSLAKVYIKPDG
                     DNVEYIPFAIYDGNLMVGFIMHAVVRETTDMYWINGFIIDEKQQGNGYGKAALQESVSL
                     IKNTCKACKEIRLTVHKDNVFAKKLYERYGFQPLGHEYDGEQVYRLFV"
     gene            4356..4802
                     /locus_tag="EXW41_RS13130"
                     /old_locus_tag="EXW41_13240"
                     /pseudo=""
     CDS             4356..4802
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="EXW41_RS13130"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_13240"
                     /product="NUDIX domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKNKFHHIVRAVMLKDNRLLLAEYIGHHYFLPGGHVEVGESAENA
                     LIREL"
     gene            5001..5264
                     /locus_tag="EXW41_RS13135"
                     /old_locus_tag="EXW41_13245"
     CDS             5001..5264
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071342.1"
                     /locus_tag="EXW41_RS13135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_13245"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_060486806.1"
                     /sec_met_domain="TIGR03601 (E-value: 3.6e-40, bitscore:
                     134.5, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQSLNLNDYQSGNVVYWDQLQQNQYPYYYIQDDARRCG
                     GCGGCGGRCGGCGGGRCGGCGGRCVGCAGCFGCFNCWNWWII"
     gene            5367..6383
                     /locus_tag="EXW41_RS13140"
                     /old_locus_tag="EXW41_13250"
     CDS             5367..6383
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="EXW41_RS13140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_13250"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_088063618.1"
                     /transl_table=11
                     /translation="MYTYDKLISWVENIKEENHSSATALCIIKDNKIVLEHYSGHHSNT
                     SISKKVTASSQFNVASARKSYLGLMVAFALYEGKINSIDDEAIQYLKDFDPVLLGKTTI
                     RHLVTHSHGLGETHDGTIFREFEPGQAWAYRDVNVRMITRLIYQLYSKSFPELLKERVF
                     DLANFKETGWRIQEDENLVKVVNNPSEDAISEIGTVNDGTEKNLFVSAREFAYWGNLHL
                     NQGMINGKQIVPKEVIKIATSLQSPTFANKELPQNGLFWFVQNEPTLLSELGERVPKGA
                     YQILGITGPTLLVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFRNGNRA"
     gene            6415..6558
                     /locus_tag="EXW41_RS13145"
     CDS             6415..6558
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="EXW41_RS13145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_087984441.1"
                     /transl_table=11
                     /translation="MKDKMNKNKKQNIKDMNTEQNAIYSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            6843..8051
                     /locus_tag="EXW41_RS13150"
                     /old_locus_tag="EXW41_13255"
     CDS             6843..8051
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="EXW41_RS13150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_13255"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_088063619.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAGKLQQKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGSLIDSLGVS
                     QAFLYWGIIVLLLVLTGSFFLREAIVSNTVTESLNNDYTPREMMRTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIVLGTLSDKIGRMKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGAFIGALLGGFQPTFIIIGVLSVISFIISIFIRPPKAENKKETRHLYR
                     KVA"
     gene            8287..8912
                     /locus_tag="EXW41_RS13155"
                     /old_locus_tag="EXW41_13260"
                     /pseudo=""
     CDS             8287..8912
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237130.1"
                     /locus_tag="EXW41_RS13155"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_13260"
                     /product="cadmium resistance transporter"
                     /pseudo=""
                     /transl_table=11
                     /translation="MVTTIISSFIAFTLTNIDDIFILLVLFSQVRTGVIKKEGSTVRGK
                     TKMKELYIVIGQYFGFSLIIFLSIIGSLSSFFIPVSWIGLLGFVPIYIGVKGLLSLRSY
                     KSNEVIANASSSIFKVASITLANGADNISIYIPMFASQNLNANIVTLVIFYCMIAIWCF
                     ISYKLIRAPILAKVLERNCHIIVPVF"
     gene            9092..9508
                     /locus_tag="EXW41_RS13160"
                     /old_locus_tag="EXW41_13265"
     CDS             9092..9508
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016104977.1"
                     /locus_tag="EXW41_RS13160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_13265"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_088063620.1"
                     /transl_table=11
                     /translation="MKIVKQWVQEDSDYIREKVIEYNQKHISDEEKKPSEKISFIVRNE
                     KEEIVGGITAITFWHHVHVDSLWVSEEYRHEGYGSQLIKLIEEFAIEQECRLINLDTFS
                     FQAPAFYKKHGYKVIGVSEDHPKGHNHYYLEKRL"
     gene            9725..10240
                     /locus_tag="EXW41_RS13165"
                     /old_locus_tag="EXW41_13270"
     CDS             9725..10240
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379366.1"
                     /locus_tag="EXW41_RS13165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_13270"
                     /product="DinB family protein"
                     /protein_id="WP_088063621.1"
                     /transl_table=11
                     /translation="MNSFVNDRFDETRKHLFEEIMILDDAQFNSKPDKNKWSIAQVCHH
                     LVLLDKVVIKVISSGLKKIDSTLKERREIRSILQDRSLKFIAPGMIEPSLEPFEVQKII
                     DMLNESRKELMRFLSTIEDESILTIKSVMHPALGELHLDQWIELIYLHEQRHIEQIKEI
                     KLLCEIGK"
ORIGIN
        1 ttatccattc agcagcaact ctttgagata aagagctcga tccatagtct gtttgcattt
       61 ttatatctga caatctatca ataactggtt cgggtccaat gatccatcca attctaagtc
      121 ctgggctaag tgttttagaa agactaccaa catataatac atgtccatgt ttatctatcg
      181 actttaaagg aggtggagat ggttcatcaa tccataactc tcggtaaata tcatcttcaa
      241 ttataggtaa ctgttccttt tcacatattt ttattatttc ttttcttcgt tcttgagaca
      301 ttaatgcccc tgttgggttt tgaaaacttg ggatagaata taaaatgttt ttcttttgac
      361 tgtatttaat acgttttaat aattcgttag gtaaaatacc gtgattatcc atacatatac
      421 cagatagatt tatatttgct gattgaaata catgaagtga atataagtaa gaaggttgtt
      481 caagtaaaac ggttgattct ctatgcaaaa gaccaattga tattaattgg agtgcttgta
      541 atgcaccaga gacgattaat atagaactgg ctgaagtatg tattccgaat gattttacgt
      601 atgtactaat tgcttcacgt aacggcaaaa aacctttttg ttcttcgtat ccaaaataat
      661 ctatttcgtc acttacgctt ttcataatgg actgcatagt ttctagcggg aaaatttgag
      721 gcgcaagttc acctttacta agatggataa gttctttatt tgcttctgct tcattgattt
      781 ctcgtaccat taacttgcta ggtttgtgaa taccagcttt tacatagtca ctccaatcag
      841 gcgggggatt cgtcgctaat aatgaccatg tattatttgt tacaattgtt cctgctccaa
      901 ttttcccttg aatcaatcca tctgccataa gttcatctag cgcagttatt acagtacttc
      961 gatttacatg aaataatttg gctaactgcc gttgactagg aatcttactt ccaataggcc
     1021 attctccatt ttcaattttt tctttcataa agtttactat ctgccgatac ttaggcagtt
     1081 ttttattagt tgtcataaaa aaactccctc gttcacttta taaatgccaa gtggttggtt
     1141 tttacgataa ataactggtt gaagacattg taacaactat taaatatgat gaaaagaaat
     1201 aattggtaat tataaagtgg ttggtttgaa gggggagaaa aaacatgaat tataaaaaat
     1261 gggactttcg gattatttgt gctcatgctt ttacgatttt aatatggggg actgcctttc
     1321 cagcaattcg tatggggctc gaatcttata cacctgagca ccttacttta ctacgtttat
     1381 taattgcttc atttattctt ctgttgtttt cttttatata caagttacga ctgccggact
     1441 taaaggatat cccagcaatt tttatatttg gtgctttagg atttactgtt tatcacattg
     1501 cattaaacta cggcgaaaaa acagtaaatg ctggatctgc aagtttaatc gtttcagtca
     1561 cacctatagt aacggctatt tttgcatctg tttttttgaa tgaaaaaatg aaattaaatg
     1621 gttggatcgg tggtgtaatt agttttatag gaattgcttt catatcattt agtcaaggag
     1681 atgctattca attgaatagt ggaggattat ttattttatt agcagcgatt tcagaaagct
     1741 tattttttgt tttccaaact tcttacttga gaaagtatgg cttcttgcca tttactatat
     1801 atacaatttt atctagcact gtatgtatgc ttattttctc gccgggagtg tatacagaaa
     1861 tactagcagc tcctcttgaa gtgaatatga gtgttatata tttaggttta tttccaacag
     1921 tactcccata tatcgcattg gcatatatca tatcccattc tggtgctgct gaggcaacaa
     1981 gttctctata tttaactcca gtaactgcat gttttattgc ttgggtatgg ttaggtgaag
     2041 taccgacttt tgtttcaata attggcgggg gaattactat acttggagtt ttaattactc
     2101 atatatcact atcaaaaaaa gaaaaatata caactctagt aaataataaa tcaatataaa
     2161 ctcattccat aaccacttta ttatactttc ttttgttgat aaatataata ttttgtattt
     2221 cagttaaata ttgtatgtta cattttatgt agtaagcatt ttaaaagggg atgacaacat
     2281 aatgaatgga aaaagaaaaa ttttcacatg tatttctatt ataggaatcg gactagctag
     2341 tttttctaat tctagtttcg cagcaaatgg aacggacaat tcagtacaaa cttctattcc
     2401 catagttaat caacaagtag ctgctgtaaa agaaatgaaa ccatttcccc agcaagttaa
     2461 ttatgcaggt gttataaaac caaatcatgt tacacaggaa agtttaaatg cttctgtaag
     2521 aagttactac gataattgga aaaagaaata tttgaaaaat gatttatctt ctttacctgg
     2581 tggttattac gtaaaaggag agattacggg tgatgctgat gggtttaagc cacttggaac
     2641 ttcagaaggt caagggtatg ggatgataat tacagtatta atggctggtt atgattcgaa
     2701 tgctcaaaaa atctatgatg gtttatttaa aacagcaaga acttttaaaa gctctcaaaa
     2761 tcctaattta atgggatggg ttgtcgcaga tagtaaaaaa gcacaaggtc attttgattc
     2821 tgctactgat ggggatttag atattgcgta ttctcttctt cttgctcata agcagtgggg
     2881 atcaaatgga acggttaatt atttgaaaga agcacaagac atgattacaa aaggtattaa
     2941 agctagtaat gttacaaata ataaccgact aaatttggga gattgggatt ctaaaaattc
     3001 acttgatacg agaccatctg attggatgat gtcacacctt agagcatttt atgaatttac
     3061 aggtgataaa acttggctta ctgttattaa taatttgtac gatgtttata cgcaatttag
     3121 taataaatac tctcaaaata caggacttat ttcagatttc gttgtaaaaa ctccaccaca
     3181 acccgcacct aaagacttct tagatgagtc agaatataca aatgcatatt attacaatgc
     3241 tagtcgggta ccattgagaa ttgtaatgga ctatgcgatg tacggcgaga aaagaagtaa
     3301 agtcgtttct gataaagtct cttcatggat tcaaaataaa acgaatggaa atccttctaa
     3361 aattgtggat ggttatcaat taaacggatc caatattggt agttatccaa ctgctgtatt
     3421 tgtttcaccg tttattgctg caagtacaac gaaaagcgat aatcaaaagt gggtaaatag
     3481 tggatgggat tggatgaaga ataagaaaga aagctacttt agtgatagtt ataatttatt
     3541 aaccatgcta tttattacag gtaattggtg gaaaccaata cctgatgata agaaaataga
     3601 aaacctaata aatgatgaaa ctcaaaaagg atacgataaa taacaaggtt ttctttttag
     3661 taaaaggaat tagcactatg ctagttcctt tttaatattt cgtgaaacaa tacagcttta
     3721 cacttaaata aagaaaaatt taataagatc atgttgtagg ttgttgtcgg aaaaaggaga
     3781 atatgtcact ctgttgaata taaaaataca acctatttat aaaggagcct aaaatattga
     3841 atgtccagtt aaaggttgtt acgagagaga attgggaaga tgccttaaag ctacaagtta
     3901 aagaaaatca gacgaaattt gttccatctg tagcagtttc acttgctaaa gtatatataa
     3961 aaccagatgg agacaatgtg gagtacatac catttgcgat atacgacggc aatctaatgg
     4021 ttggttttat tatgcatgct gttgtaagag aaacaactga tatgtattgg ataaatggat
     4081 ttattatcga tgaaaagcag caagggaatg gttatggaaa agcggcatta caagaaagtg
     4141 tttctttaat aaaaaataca tgtaaggcgt gtaaagaaat aagattaaca gtacataaag
     4201 ataatgtctt tgcaaagaaa ctatatgaac gttacggttt tcaaccattg ggacatgaat
     4261 atgatggtga gcaagtatat cgtttgttcg tttaagtatt acgaagtaaa gatgtgaaat
     4321 gaaagatgcc attaaatgac tgggaggaat agcaattgaa aaataaattt catcatattg
     4381 tacgagctgt tatgttaaag gataatagat tattattggc tgaatatatt ggccatcatt
     4441 attttttacc aggtggtcat gttgaagttg gcgagtcagc tgagaatgca ttaataagag
     4501 aactatgaga agaactcgga gtaaattgta gtataaaaca atttttaggc gtcatagaaa
     4561 accaatggca agaccatgaa gttcttcatc atgaaatcaa tcatattttt gaagtagatt
     4621 cgcaagagtt acatattgat ttcataccaa aatctaaaga atctcattta gcgtttcact
     4681 ggatagatta taatcaagag gctttacata cgtataaaat catgccagta ccttcagtta
     4741 aagagttact agaaaggaaa ttaagcgatg aacgactaaa ctgttggatt agcaattttt
     4801 aaacacctct attaatagta aatacgtccc tgaaaggaga ttaagaaaat aggctgaaaa
     4861 acaaggtaca tcacctagtg taagttcata ttttgttgat gtacgcattt agtctatagc
     4921 cttatttcta ctttgtttgc aaaaatatat aaactttaac attgggacgg aatttatttt
     4981 tataaatgga gggtttcgta atgaatcagt ttcaacaaga actacaatca ttaaacctta
     5041 atgattatca atctggtaat gttgtgtatt gggatcaact acaacaaaat caatatccat
     5101 actattacat tcaagacgat gcacgtcgtt gcggcggatg cggaggttgt ggtggtcgct
     5161 gtggtggatg tggcggcgga agatgtggag gatgtggtgg tcgttgtgta ggctgcgctg
     5221 gatgtttcgg ttgttttaac tgctggaact ggtggatcat ttaagtatag aaaactaaca
     5281 atggtgtaca tgaaagaaag gtgtaaatac tattttatac ttttctttca tgttgtaatt
     5341 ttttattcgt gttaaggggg atttatttgt atacatacga taagttaatt tcttgggtag
     5401 aaaatataaa agaagaaaat catagttctg ctacagcact gtgtataata aaagataaca
     5461 aaatcgtact agagcattat agtggccatc attcaaatac atctataagt aaaaaagtaa
     5521 ccgcatcttc acaattcaac gttgcttctg cgagaaaaag ttatttaggt ttaatggtag
     5581 catttgcgct ttacgagggg aaaataaact ctattgatga tgaagcgata cagtatctta
     5641 aagactttga tcctgtatta cttggtaaaa caacaataag acatttagta acccattcgc
     5701 acggtttagg agaaacgcat gatgggacaa tttttcgtga atttgaacct ggacaagcat
     5761 gggcgtatag agatgttaat gtcagaatga taacacgtct tatttatcag ctatatagca
     5821 aaagttttcc tgaattgtta aaggagcgtg tatttgatct agctaacttc aaagaaacag
     5881 gttggagaat tcaggaagat gaaaacttag ttaaagttgt taataatcca agtgaagacg
     5941 cgattagtga aatcggtacg gtaaatgatg gtactgaaaa aaatctattt gtctcagcta
     6001 gagaatttgc ttattggggc aatcttcatt taaatcaagg tatgataaac ggtaaacaaa
     6061 ttgttccaaa agaagttata aaaattgcta cgagtttgca aagtccaaca tttgcaaaca
     6121 aagaactacc acaaaatggg ttattttggt tcgttcaaaa tgaacctacg cttttaagtg
     6181 aacttggtga acgagtgccg aaaggagcgt atcaaatatt aggaattaca ggaccgactc
     6241 ttttagtaat acctgaatat aatgtagttg ttgcgaaaat gtataataaa aggtataact
     6301 acggcggtga taattattta tattatttac gtgaatttag caatttagtc gctgatacat
     6361 ttcgtaacgg taatagggca taattactcc tataatgaaa aaggagtgaa aaaaatgaaa
     6421 gataaaatga ataaaaataa aaaacaaaat ataaaagata tgaatacaga acaaaatgca
     6481 atttatagcg atcctaaaga tgcagctaat atgcaaacgg taccgcagcc gaaggatttt
     6541 gatgaaattg aatattaatt tttcgttcat ccgactatat gatgaatgaa aaatacatct
     6601 taagtcccat cacatgattg aaatcttttg gtattctata tacataagca tttgaaaatg
     6661 tcttatctta acaaagcttt ctttaagtaa atctaatata acctgcagaa agacacttca
     6721 ttatggagtg tctttttgtg tacatgaatt caatttccat ttttcatgca gtatcttgct
     6781 tcttatgatg gttatactaa ctgtgaaaat tcatgtcatg ataaaagaag gggacagtta
     6841 gtatgaaaca aacttctata aatccgttac taattgttct aggtacaatc attgttcaaa
     6901 ttggccttgg gacaatttat acatggagtt tatttaatca accccttgtt agtaagtttg
     6961 gatggaacct caattccgta gcgataactt tctccataac aagtttttca ttatcattct
     7021 caaccttatt tgcaggaaag ttgcagcaaa aattaggact tcgtaaactc atcgctactg
     7081 cagggattgt tttgggactc ggtttaatac ttagttcaca agtttcttcc ttaccattac
     7141 tatatttatt agctggtgtc gttgttggtt atgcagatgg aactgcttat attacatcac
     7201 tatctaattt aattaaatgg tttccaaatc gaaaaggact tatttctggc atatctgttt
     7261 cggcatatgg aatgggcagc ttaatcttta gatatataaa cggaagtctt atcgatagtc
     7321 ttggtgtatc acaagcattt ttatattggg gtatcatcgt cttgctttta gttttaaccg
     7381 gatcgttctt cttacgtgaa gcaatcgtaa gtaacactgt aactgaatca ttaaacaatg
     7441 actatacgcc tcgtgaaatg atgcgaacga aacaagtata tcttttgttt tttatgctat
     7501 tcacatcgtg tatgggcggt ctatatttaa tcggtatggt aaaagatatt ggggtacagc
     7561 tcgttggact tagtgcagca accgccgcta acgccgttgc aatgattgca atttttaata
     7621 cggtaggtag aatcgttctt ggaacgttat cagataaaat tggtcgaatg aaaattgttt
     7681 ctgcaacgtt tataataatt ggattgtcag tctttacttt aagtttcatt cctctaaatt
     7741 acggtattta tttcgcttgt gtagcaagtg ttgccttttg ctttggtggt aatatcacaa
     7801 tcttcccagc cattgttgga gatttcttcg gattaaaaaa ccatagtaca aattacggga
     7861 ttgtatatca aggtttcggg tttggtgcac ttgcaggagc gttcattgga gcgttactgg
     7921 gtggatttca accgaccttt attataattg gtgttttaag cgttatttcc ttcattattt
     7981 cgatatttat tcgtccacca aaagcagaaa acaaaaaaga aacacgacat ttatatcgga
     8041 aagtagctta attataaaaa gagtccttgt atagggctct ttttatatta caaaataagt
     8101 gaaaatgatt tgaaaggtaa taaagtaatt tatttcttta gcaactaagt agcagattag
     8161 taaaatattc atattgtttt ataaaggcac tgaaatcatg ttataaacct acattaacca
     8221 cctttgttat tagatatgca attcatttgt acaccattat tttattataa aggtagttgg
     8281 cttatattgg ttacaactat aatttcttct tttatcgcat tcactttaac aaatattgat
     8341 gacattttta tacttcttgt tttattttcg caagtaagga caggagtaat taagaaagaa
     8401 ggtagtactg tccgagggaa aactaaaatg aaagaacttt atattgttat tggacaatat
     8461 tttgggttta gtttaattat ttttctaagt atcatcggtt ctttaagctc ttttttcatt
     8521 cctgtttcgt ggatcggatt attaggattc gtaccaattt atattggagt gaaaggacta
     8581 ttgtcacttc gttcttataa aagtaacgaa gtcattgcta acgcttctag ttcaatattt
     8641 aaagtagctt caattacatt agctaatgga gctgataata tttcgattta tataccaatg
     8701 tttgctagtc aaaatcttaa tgccaatatt gttacattag taatctttta ttgcatgata
     8761 gcaatatggt gttttattag ctacaaattg ataagagctc ctattttagc taaagtactt
     8821 gagagaaatt gtcatattat cgttccagtg ttctaattgg tttaggaatg ttcattctta
     8881 tccgcagtaa tacaattgaa ttattgtact aatatgttga aacttagatt aaaaagaatc
     8941 agacgccata aaaagccgta ctacagataa aatgtagtac ggcttttctt tattttagtg
     9001 aaatgaaaca gtgctctacg actattaagt tagacaggat attacgatta ttaaattgaa
     9061 tagatacttt aaaaggagtg tgctacttaa aatgaaaata gtaaaacaat gggtccaaga
     9121 agatagtgat tacataagag aaaaagtaat tgaatataac caaaaacata tttcggatga
     9181 agagaaaaag ccttcagaaa aaattagttt tatagtgaga aatgaaaagg aagaaattgt
     9241 aggcggaata actgcaatta cattttggca ccacgtacat gtcgattccc tttgggtttc
     9301 tgaagagtat agacacgaag gctacggaag ccaattaata aagcttattg aagaatttgc
     9361 aattgaacaa gagtgtaggc taatcaattt agatactttt agctttcagg cacctgcgtt
     9421 ttataaaaag catggatata aagtgattgg agtaagtgaa gatcatccga aaggacataa
     9481 tcattactat ttagaaaaac gtttataaag tgtatattga attaacatta gtattttgaa
     9541 tacaaattta tccttttgga ttatgcattt aaaggagaat cttgagtgaa ttcatattta
     9601 gaggcacttc taaagcaaaa aaagatcgaa gtggaatatt agttacggga aactaattat
     9661 ttatagcgaa tatacctaaa ataggcatct aaatatatgc attcagatga aggggggaaa
     9721 tgttatgaat tcatttgtaa atgataggtt tgatgaaacg agaaaacatc tattcgagga
     9781 aattatgata ttggatgatg ctcaattcaa tagtaagcca gacaagaata aatggagtat
     9841 agcacaagta tgtcatcact tagttttatt ggataaagtg gtcataaagg ttatttcatc
     9901 tggcttaaaa aagattgata gtaccctaaa agagcgtcga gaaatccgct ctatattaca
     9961 agatagatcg ctaaaattta tagctccagg aatgattgaa ccaagtttag aaccatttga
    10021 agtgcagaaa ataatcgata tgttaaacga atcgagaaaa gaattaatgc gtttcctaag
    10081 tacaatagaa gatgaatcta tattaacgat taaatcagtg atgcatccag ctcttggaga
    10141 attacatctt gatcagtgga tagaactgat ctatttgcat gaacagcgtc atatagaaca
    10201 aatcaaagag ataaaactac tttgtgaaat tgggaagtaa aaaatctttg gctattaaca
    10261 aaaa
//
