LOCUS       NZ_CP036070            10333 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Bacillus wiedmannii strain JAS07/5 chromosome, complete genome.
ACCESSION   NZ_CP036070
VERSION     NZ_CP036070.1
KEYWORDS    RefSeq.
SOURCE      Bacillus wiedmannii
  ORGANISM  Bacillus wiedmannii
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5239489)
  AUTHORS   Fiedoruk,K., Drewnowska,J.M. and Swiecicka,I.
  TITLE     The dynamics of pangenome and proteome of selected Bacillus cereus
            sensu lato representatives from the primary and anthropologically
            changes environments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5239489)
  AUTHORS   Drewnowska,J.M., Fiedoruk,K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2019) Department of Microbiology, University of
            Bialystok, 1J Ciolkowskiego, Bialystok 15-245, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.157x
            Sequencing Technology :: Illumina MiSeq and MiniIon Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018741285.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 04:17:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,980
            CDSs (total)                      :: 5,827
            Genes (coding)                    :: 5,631
            CDSs (with protein)               :: 5,631
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 88 of 196
            Pseudo Genes (incomplete)         :: 110 of 196
            Pseudo Genes (internal stop)      :: 59 of 196
            Pseudo Genes (multiple problems)  :: 52 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:47:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2512897
            Orig. end                         :: 2523230
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036070.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10333
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10333
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10333
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5333](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5333
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            145..1188
                     /locus_tag="EXW41_RS12675"
                     /old_locus_tag="EXW41_12775"
     CDS             145..1188
                     /EC_number="1.1.1.3"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990711.1"
                     /locus_tag="EXW41_RS12675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_12775"
                     /product="homoserine dehydrogenase"
                     /protein_id="WP_088063565.1"
                     /transl_table=11
                     /translation="MKVQVVLSGYGTVGREFIKLLNEKYLYINESYGIDLVVSAVLGRN
                     VAIHNEDGLSIHHLLMYGGGSAAIEEYIEHHPEERATTSISGTVLVESTVTNLKDGNPG
                     KQYIKQAIENHMDIVAISKGALVTNWREINEAAKIANVRIRYSGATAAALPTLDIGQFS
                     LAGCHIEKIEGILNGTTNYILSKMNEEDITFEEALKEAQSKGIAETNPILDVSGSDSAC
                     KLLLLTNSLMGTENTLTDIHIKGIEHVTKQQIRNAKEQNKYIKLIASAYKDKDGKVALH
                     VEPYEIDKDHPLAKVNGTEKGITFFTDTMGQVTTIGGASNPRGAAAAALKDVINLYRKD
                     LSLSNRQ"
     misc_feature    1259..1273
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.35"
                     /tool="antismash"
     gene            1349..2797
                     /locus_tag="EXW41_RS12680"
                     /old_locus_tag="EXW41_12780"
     CDS             1349..2797
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-469). E-value:
                     1.4e-31. Score: 101.8. Matches aSDomain:
                     nrpspksdomains_EXW41_RS12680_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="EXW41_RS12680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_12780"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_046959518.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.2e-29, bitscore:
                     101.9, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYLKKMIMKGHLLGDQKIPSTRELSEL
                     LSVSRNTVLAAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKHKLNTVTLLADE
                     LDLMKHGVRWEKGMIVFNSIAPDEKLFDVENFKRAFLTRMSIEGDVVLNYGYAKGYRPL
                     MNYLLHYMEMKGVDISNKDILITNGFTEGLDIVLSSLSKKSGRVICENPTHHAALKLFR
                     LHGLEVHGINMNEDGIDTNEVEKSLREKEFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYKVPIIEDGFNEELRYSGSHLAPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHHLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNEY
                     IPFQRMTGDGGLHLFIELEEKIHARTLLQKCYEQGVTFSPGDVFYSDGGGATTFRLGFS
                     RLKEEEIVRGIKIIGDTIKTEIWS"
     aSDomain        1784..2755
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EXW41_RS12680_Aminotran_1_2.1"
                     /evalue="1.40E-31"
                     /label="EXW41_RS12680_Aminotran_1_2.1"
                     /locus_tag="EXW41_RS12680"
                     /protein_end="469"
                     /protein_start="145"
                     /score="101.8"
                     /tool="antismash"
                     /translation="IEGDVVLNYGYAKGYRPLMNYLLHYMEMKGVDISNKDILITNGFT
                     EGLDIVLSSLSKKSGRVICENPTHHAALKLFRLHGLEVHGINMNEDGIDTNEVEKSLRE
                     KEFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYKVPIIEDGFNEELRYSGSHLAPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHHLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNEYIPFQRMTGDGGLHLFIELEEKIHARTL
                     LQKCYEQGVTFSPGDVFYSDGGGATTFRLGFSRLKEEEIVRGI"
     gene            2802..3716
                     /gene="ddlB"
                     /locus_tag="EXW41_RS12685"
                     /old_locus_tag="EXW41_12785"
     CDS             2802..3716
                     /EC_number="6.3.2.4"
                     /codon_start=1
                     /gene="ddlB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="EXW41_RS12685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_12785"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_088063566.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 8.2e-22,
                     bitscore: 76.0, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 1.2e-12, bitscore:
                     46.4, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 2.2e-08, bitscore: 32.2,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 4.7e-51,
                     bitscore: 171.4, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 2.2e-05, bitscore:
                     22.4, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIGVIMGGVSSEKQVSIMTGNEMIANLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSGICMDKNISKKILRY
                     EGIETPDWIELTKMADLNLDELEKLGFPLVVKPNSGGSSVGVKIVYDKDELISMLETVF
                     EWDSEVVIEKYIKGDEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDVSTIEEVIELPG
                     ELKERVNKASLACYKALKCSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAG
                     IHYSKLLDMIIETSLKARKEEGF"
     regulatory      3789..3894
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     misc_feature    4017..4029
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     gene            4017..4706
                     /gene="tenA"
                     /locus_tag="EXW41_RS12690"
                     /old_locus_tag="EXW41_12790"
     CDS             4017..4706
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="EXW41_RS12690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_12790"
                     /product="thiaminase II"
                     /protein_id="WP_088063567.1"
                     /transl_table=11
                     /translation="MTFSQSLRQEVDSIWEASFHHPFVKQLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITQEEKDNFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWISAY
                     GSDWFRTLVEEQITRLDTIAEKVTKSDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVNT
                     EVEDVIG"
     gene            5001..5333
                     /locus_tag="EXW41_RS12695"
                     /old_locus_tag="EXW41_12795"
     CDS             5001..5333
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002059557.1"
                     /locus_tag="EXW41_RS12695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_12795"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_215559071.1"
                     /sec_met_domain="TIGR03601 (E-value: 9.4e-39, bitscore:
                     130.0, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKMNKFQQELQALSLNDYQSGNIVYWDQQNQYPYYYIEDAARRCG
                     GCGRCGGCAGRCGGFRCGGFRCIGCFGCGGCGGCGGCGGCGSCSNCFSGFNGTTDTTGT
                     IVTYEY"
     gene            5702..6181
                     /locus_tag="EXW41_RS12700"
                     /old_locus_tag="EXW41_12800"
     CDS             5702..6181
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571439.1"
                     /locus_tag="EXW41_RS12700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_12800"
                     /product="homoserine dehydrogenase"
                     /protein_id="WP_176549881.1"
                     /transl_table=11
                     /translation="MIELKSIIHSYKLKRKIARDLYGNRDELTLLLNEFNKMKHTVTCE
                     KKKNNILSRLQLIYQHMKLDKRYPLPITFNSKLLDRLEKESLHSIEEGIACLQLMLDMN
                     YEKIKQYGSSTSRSFVPLSQSSICLADCICITGFVLGLLGAITFGGLVLSVCSIT"
     gene            6425..6550
                     /locus_tag="EXW41_RS29920"
     CDS             6425..6550
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EXW41_RS29920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_255258668.1"
                     /transl_table=11
                     /translation="MNKFEWVIAILEIVKPYMDEKDFEQVMFKTVVLMQEEMDIK"
     misc_feature    6615..6632
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     misc_feature    complement(6670..6685)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 23.85"
                     /tool="antismash"
     gene            7169..7345
                     /locus_tag="EXW41_RS12705"
     CDS             7169..7345
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221760.1"
                     /locus_tag="EXW41_RS12705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6877 family protein"
                     /protein_id="WP_170958655.1"
                     /transl_table=11
                     /translation="MTYLEQINKLSSQLPLVVLQDIHNHVRDWLASGGEENDSYIGQKL
                     RFAENYLKARGTE"
     gene            7342..7551
                     /locus_tag="EXW41_RS12710"
                     /old_locus_tag="EXW41_12805"
     CDS             7342..7551
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EXW41_RS12710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EXW41_12805"
                     /product="hypothetical protein"
                     /protein_id="WP_252196031.1"
                     /transl_table=11
                     /translation="MKCKHKWIDMEDGTNDKFCVKCSQKVKQMQIALPISISISVSAAL
                     PMAREKMTIISYGSLQEFYKDEGV"
     gene            complement(8049..8333)
                     /locus_tag="EXW41_RS12715"
                     /old_locus_tag="EXW41_12810"
     CDS             complement(8049..8333)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123246.1"
                     /locus_tag="EXW41_RS12715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_12810"
                     /product="DUF4183 domain-containing protein"
                     /protein_id="WP_001123250.1"
                     /transl_table=11
                     /translation="MPIVKPFIAGRRFVSTAATGTVAGADLTFANTDFTDDTGAVTTFP
                     ASYAFLTLYINGVIQTGDTITGVTTTAATIVGGAVLDGGTPIAIEFTIT"
     gene            8544..8753
                     /locus_tag="EXW41_RS12720"
                     /old_locus_tag="EXW41_12815"
     CDS             8544..8753
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002119200.1"
                     /locus_tag="EXW41_RS12720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_12815"
                     /product="hypothetical protein"
                     /protein_id="WP_142291749.1"
                     /transl_table=11
                     /translation="MNQLSFFKDIDEKEMHRLVVKELKNYKALCVRMKNQEEQARAGGI
                     VLFPKMKGDEKNHEIRVKQIERTL"
     misc_feature    complement(9005..9021)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.77"
                     /tool="antismash"
     gene            9138..9734
                     /locus_tag="EXW41_RS12725"
                     /old_locus_tag="EXW41_12820"
     CDS             9138..9734
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016092669.1"
                     /locus_tag="EXW41_RS12725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW41_12820"
                     /product="hypothetical protein"
                     /protein_id="WP_000122592.1"
                     /transl_table=11
                     /translation="MSYSYYDHCKDNKKKYHDCHKSSKSYDKCDKKHDEKPWVNVKVNC
                     CSDDNQYNLVRASAFRAVSTVNQIVPANTFVKVLFQNEQFDLANEYNPATSIFIPKTRG
                     VYSVIGTIAFIPNNLNVNYRARVEIRVNGNPAIAIDNDFFGPINFANVVAVSSIIQLNA
                     GDVVEVFAQSSVAGVISTVENSTHFEAARFPSPTE"
     misc_feature    9864..9882
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 26.59"
                     /tool="antismash"
     misc_feature    complement(9865..9883)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 26.56"
                     /tool="antismash"
ORIGIN
        1 agcattcaag agttttatag aacaattgca gaaaaattat caagattacc gaaataggta
       61 ataataaagc gctctcgatg gaattcgagg gcgctttata ttatattgaa agagggatat
      121 ggtagaaaaa tatgggggga tattatgaaa gttcaagttg tattatctgg atatgggaca
      181 gtaggaagag agtttataaa attattaaat gaaaaatatt tatatataaa tgaatcatat
      241 ggaatagatt tagtggtaag cgctgtatta ggtagaaatg ttgcaataca taatgaggat
      301 ggattatcta ttcatcactt attaatgtat ggtggcggta gcgctgcaat tgaagaatac
      361 attgaacatc atccagagga acgagcaaca actagcataa gtggaactgt attagtagaa
      421 tcaacagtta caaaccttaa agatggaaat ccaggaaaac aatatataaa acaagcgatt
      481 gagaaccata tggatattgt agcaatttca aaaggcgcac tcgttacaaa ctggagagaa
      541 ataaatgaag cagcaaaaat cgcaaatgta cgaattcgat atagtggtgc aactgcggcg
      601 gcattaccga cacttgatat tggtcaattt agtttagctg gttgccatat tgaaaaaata
      661 gaaggaatat taaacgggac tacaaattat atcctttcga aaatgaatga ggaagatatt
      721 acgtttgaag aagcactgaa agaagcgcaa agtaaaggaa ttgctgaaac gaacccaata
      781 ttagatgtaa gtggttctga tagtgcgtgt aaattgttac ttttgacaaa cagtttaatg
      841 ggaacagaaa atacgcttac tgatatacat ataaagggta ttgaacatgt tacaaaacaa
      901 caaattcgaa atgctaaaga acaaaataaa tatattaaat taatagcttc agcatataag
      961 gataaggatg gtaaagtggc tcttcatgtg gaaccgtacg aaatagataa agatcatcca
     1021 ctagcaaaag taaatggaac agaaaaagga attacgttct ttacagatac gatggggcaa
     1081 gttactacaa ttggtggtgc ttccaatcca cgtggagccg cagcagcagc tttaaaagat
     1141 gtaattaact tataccgcaa agatttatcc cttagtaata ggcagtaaaa tgcctgttac
     1201 taagggataa taatcagtgg gtgataatca gccccattga ttaaagttcc actttataag
     1261 accgtaccgc cctttataaa aaatacatat gtagtggacc ttttgagaat gaaataaata
     1321 tgcaaaaatg attgcagggg gtaaggaatt gtttaaagat tttaaagttg tgaaagatcg
     1381 tcccgtttat attcaattga aagattattt gaaaaagatg attatgaaag gtcatttgct
     1441 cggggatcaa aaaatccctt caactaggga actaagtgaa ttactaagtg tgagcagaaa
     1501 tactgtactc gctgcttatg cggatttaga gcaagaagga ctcatttatg cagttaaagg
     1561 gaaagggaat tttgtagcga aggttgatat atcgaacact tcgtctgttg aaatagattg
     1621 gaaacataaa cttaatacag ttaccttatt agcggatgaa ttagatttaa tgaaacatgg
     1681 tgttcgctgg gaaaaaggaa tgattgtttt taatagcatt gctccggacg aaaagctatt
     1741 tgatgtggaa aatttcaaaa gagcttttct tactcgtatg tccattgaag gggatgtcgt
     1801 attaaactac ggatacgcaa aaggttatag acctttaatg aattatctac ttcattatat
     1861 ggaaatgaaa ggtgtagata tttcgaacaa agatatttta attacaaatg gatttacaga
     1921 aggattagac atcgtgctat cctcgttatc gaagaaatca gggcgggtta tttgtgagaa
     1981 tccaactcac catgctgcat tgaagctttt ccgtttacat ggacttgaag ttcacggaat
     2041 taatatgaat gaagatggca tcgatacgaa cgaagtagaa aaaagtttgc gtgaaaaaga
     2101 gtttgatttc gcatatttaa ttccttccta tcataatcca accggtattg ttacgagttc
     2161 ggagaaaaga acggaactga tgagattatt ctccaaatat aaggttccta ttatagaaga
     2221 tggatttaat gaagagctac gttattcagg ttcacactta gcaccattat taacttttgc
     2281 tggcgctggt aataatgtaa tttatattag tagcttttca aaggtacttt tccctggttt
     2341 acgtgtaggc tggatcatcg ccgataaaga actgattcat catttagaaa gtgtaaaaag
     2401 agcaagaacg attcacacat ctacgttaga tcaagctgtt ctatttcaat atttacatga
     2461 aggatatttt gaaaaatatt taaaaaaggc aagatcagtt tataagaaga aatatgaatt
     2521 agctgttggg gcgtgtaacg agtacattcc gtttcaaaga atgaccggtg atggtggact
     2581 tcatttattt atagagttag aagaaaaaat tcatgcccgc acacttttac aaaagtgtta
     2641 tgagcaaggg gttacatttt ctccaggaga tgttttttat tctgatggag gaggagcgac
     2701 tacttttcga ttaggatttt cacgtctgaa agaagaagaa atagttcgtg gaatcaaaat
     2761 aatcggtgat acaataaaaa ctgaaatttg gagttgagga aatgagaatt ggcgttatta
     2821 tggggggagt atcctctgaa aaacaagtat caattatgac tggaaatgaa atgattgcaa
     2881 atttagataa aaataaatat gaaatcgtac caattacgtt aaatgaaaaa atggatttaa
     2941 tcgaaaaagc gaaagacatt gattttgcgc tgcttgcatt acacggaaaa tatggagaag
     3001 atgggacggt tcaaggcacg cttgaaagtt taggtattcc atatagcggg agcaatatgt
     3061 tatctagcgg tatatgtatg gacaaaaata tttcgaaaaa gattttacgc tatgaaggta
     3121 tagaaacacc cgattggatt gaacttacaa aaatggcgga tctaaatctt gatgaattag
     3181 agaagttagg atttccgtta gtggtaaaac caaactctgg tggatcaagt gttggggtaa
     3241 agattgtata tgataaagat gaattaattt caatgctgga aactgtgttt gaatgggatt
     3301 ctgaagtagt aatagagaag tatataaaag gtgatgagat tacatgttca atttttgatg
     3361 gaaaacagtt acctattatt tcaatccgac atgcagctga gttctttgat tacaatgcaa
     3421 aatatgatga tgttagtaca attgaagaag ttatcgaact tccaggagaa ttgaaggaac
     3481 gtgtaaacaa agcgtcactg gcttgttata aagcattgaa atgcagcgtt tatgcaaggg
     3541 tggatatgat ggtaaaggac ggaattccat atgtaatgga ggttaataca ttaccaggga
     3601 tgacacaagc aagtttacta ccgaaaagtg cagatgcagc tggtattcat tatagtaaac
     3661 tattagatat gattattgaa acttcattaa aagcaagaaa agaagaagga ttttaagaag
     3721 cgctatcatg aatacttttg acagtaacat gaagtatgtt atgatgaata caatacgaaa
     3781 attaaatatt tatccactag gggggcctat tataggctga gatcaaatga gaatttgaga
     3841 ctcttagtac ctgatctggt taatactagc gtagggaagt gggaagacat tactatttta
     3901 tgaataaata ctgtcaattt cactttcttt acgtttgtaa agaaagtttt tttattttac
     3961 agatttaata actgtgggta aatacttaaa ttttatactt tattggaggg atttaaatga
     4021 cattttcaca atcattacgt caagaagtag attcaatttg ggaagctagt tttcatcacc
     4081 cttttgtaaa acaacttggt gaaggaacgt tagatttagc tagttttcgc tattacgtac
     4141 ttcaagattc atattatttg agtcacttcg ctagagtaca aactttagga gctgcaaaag
     4201 cactggaatt agaaacgaca gctcgtatgg cacaccatgc tcaaaataca tacgaggcgg
     4261 aattatcttt acatgagaac tttgcgaaga aactagggat aacacaagaa gagaaagata
     4321 attttatccc tgctccaact gcatacgctt atacttcaca tatgtatcgt gctgcgtatg
     4381 aaggacattt aggcgatatc attgcagcaa ttttaccttg ctattggttg tattatgaaa
     4441 ttggggagcg tttaaaagaa tgtcagccag aagagccaat ttataacgaa tggatttctg
     4501 cttatggatc tgattggttc cgtacattag ttgaagagca aattacacgg ttagatacta
     4561 tcgctgaaaa agtgacaaag tctgaccgga agcgaatgaa acagcatttt attattagta
     4621 gtcaatatga atattcgttt tgggaaatgg cttatacttt agaaaagtgg ccagtgaata
     4681 cagaagtaga agatgtcatt ggataatgag taggggtatt gctatacata ggtatggata
     4741 atacttatgt atatgatttc acaaaagaag tatcgcaaaa agacgcatca atctaagtgc
     4801 gtcttttttc aatttataaa aatgagccta tggaaaaaat aacctaaaaa taggtatatg
     4861 aacaaggtgc attatctagt gataggtcat attttgtagg ggtacgtatt gaacttagtt
     4921 tatagcgcta tttctactta gatttaacga acagaaatat aagaaagcat tggaaaagtt
     4981 gaagtttata aacggaggta atgaaaatga ataagtttca acaagaacta caagcgttaa
     5041 gccttaatga ttaccaatct ggaaatattg tgtattggga ccaacaaaac caatatccat
     5101 attactatat tgaagatgct gctcgtcgct gtggcggttg tggtcgttgt ggaggttgtg
     5161 caggtcgctg tggtggattt cgttgtggtg gatttcgttg tattggttgc ttcggttgtg
     5221 gaggttgtgg aggttgtggc ggctgtggcg gctgtggtag ttgttctaat tgttttagtg
     5281 gttttaatgg tactactgat acaactggta ctattgtaac atatgaatat tgataagaga
     5341 aaaaaggaat ccaatagtga agtattatat aatttttatt aaagaattta ataaaaacac
     5401 atgtagcatt caagtgaaag aatgatattt agatttttag ttgtaaatca agataagata
     5461 tataaagcaa tgactagact taaaacctaa tcattgcttt tttatatttt taccatcaaa
     5521 taaggaatct gttgcttcta gttagtgggt agcgaaagca attattacaa ctatgtaaga
     5581 ttaatgtaat gttaatcgat agtaaatgaa gatacttgag tgtattttaa ataagattta
     5641 aaagtactgt ttatttttaa aatattgagg taagtaaaaa tctattagga agtgtattaa
     5701 aatgattgaa cttaaatcta ttatccattc gtacaaacta aagagaaaaa tagcaaggga
     5761 tttatacgga aacagagatg aattgacact gttattaaat gaatttaata aaatgaaaca
     5821 cacagtaaca tgtgaaaaaa agaaaaataa tatattatct cgtttacaat taatttatca
     5881 acatatgaaa ttagataaac ggtatcctct tccaattact tttaatagta aattattgga
     5941 tcgattagaa aaagaatctc ttcattctat tgaggaaggt atagcatgtc tacaattaat
     6001 gttagatatg aattatgaga aaataaaaca atatggatcg agtacaagta ggtcatttgt
     6061 tccattatcg cagtcttcca tttgtcttgc tgattgtatt tgcataactg gatttgtatt
     6121 aggattacta ggtgcaatta catttggggg attagtttta tctgtatgtt caattacata
     6181 aaggttgata tttgcaagaa gggcaccgat ttaaggtgtt cttttttagt tttattgtaa
     6241 atgataaata ttacatcatt cttatcgcaa caaagtagtt cagcccctaa cggtatgcgt
     6301 caaggtgctg aaatttgaga acccttcttt aatagataaa tatgattatc aatctcttgc
     6361 attataacac taggttaatt aaatgagaaa cttaatattt atatgtagtt atataagtga
     6421 gaatgtgaac aaatttgaat gggttattgc gattctagag attgtaaagc catacatgga
     6481 tgaaaaagat tttgagcaag taatgtttaa aactgtagtt cttatgcaag aggaaatgga
     6541 tattaaataa aattgttatt tcattgtaag taaaaagagc acacatataa gcatgctctt
     6601 cgataagaaa ggtagatccc tatgagcgga gagactacat acaatatcgt atgttcgttt
     6661 cttttaaatg tgaagaaaaa gtcaacaaaa ggtcaacgta aagtcaatga aaattcaata
     6721 aaaatactat ttggtaagta catgtaaaaa agagcgcctt gtaaaggcac actttgtaaa
     6781 gtttttttct actgtcgtgt ttttttatag agttgaaagc tattatccca cttataaaac
     6841 taggcagtgg gctccaaaag aagaacccct atcgaccagt tactagggtg acagttaaga
     6901 aaaggagtgg tactaaaata atcattataa aagatattct ttgatatgga tttatataaa
     6961 aacgcccctt tatagatata tagtagtgta tttatattta caattcagta gctttgaaag
     7021 ttcattattt ttagtaaaga tttcacttgg tcacaaataa aagagcagct agcaaaagct
     7081 aactgctttt ataagaaaat cggagaaaga taaccatgtg tctaaagtat tgacggaatt
     7141 ttgagtttta ttcaggagag agaaagaaat gacatattta gagcaaataa ataagctttc
     7201 ttcacaatta cctttagtag ttcttcaaga tattcataat catgtaagag attggttggc
     7261 aagtggtgga gaagaaaatg attcgtatat tggtcagaaa ttacgattcg cagaaaatta
     7321 tctaaaggcg agagggacag aatgaaatgc aaacataagt ggattgacat ggaagatgga
     7381 acaaatgaca agttctgtgt gaaatgtagc caaaaggtga aacaaatgca aattgcatta
     7441 cctataagta tttctattag cgttagtgca gctttaccaa tggcgaggga gaaaatgact
     7501 ataatctcat atgggagttt acaagaattt tataaagatg aaggagttta ataaagaaag
     7561 aggattcgga attcaaccct tgggaaatgc aacaaaatag ttattttaat aaaagaaacc
     7621 ccgattgttt gcggggtttc taagggtaat cgtcaagtaa tgaggtaatc gactaattaa
     7681 ccatatcatg agtttttttg taaatgtgtc cgaatagata aggtcatcat tttgaacaaa
     7741 aacgctattt tataaaaaag agaagcgctt aatcaagacg cttctcttaa tgccaatata
     7801 caataactat tccattataa tgtataagcg taaaatattt aacaattagc tatgtggtaa
     7861 aaatttcatt ttgtattagt ttaaaactaa aaaagagcac catataagtg ctcttttcaa
     7921 gagtgagtat aaataccaat catcctataa tagtatatgc accgcattat gtagttgcta
     7981 ctaaaattaa cttcattttt taaaattaga ggtttctttt aatgaaacct ctaaaaagat
     8041 aactaaattt acgttattgt aaattcgatt gcgataggag ttcctccatc taggacagct
     8101 cctcctacaa tagtagcagc tgtagtagtc acaccagtaa tagtatcccc tgtttgaata
     8161 acgccattaa tataaagtgt taaaaaagca taagaagcag ggaatgttgt tacagcacca
     8221 gtgtcatcag tgaagtctgt gttagcaaaa gttaaatctg ctccagcgac agtccctgtt
     8281 gctgctgtac ttacaaatcg tcttccagct ataaagggct taacgatagg catttatgtt
     8341 cacctctttt ttgagaataa actcagacaa gttttttgaa agtcttgtcc tgtattttat
     8401 tctatgttgt gcttctttta aaagatacgg cttgtatact agtaaaaaat tataattatt
     8461 atgtgtttaa tagatgctgt ataaatttaa acaaaataat tcgtttataa gtgagtagat
     8521 cgaacggagg ggaaccgtat cgtatgaatc aattgtcatt ttttaaagac atcgatgaaa
     8581 aggagatgca tagacttgtg gtgaaggagc tgaaaaacta caaagcattg tgcgtccgga
     8641 tgaaaaatca agaagaacag gctcgagcgg ggggcatcgt tctttttcct aaaatgaaag
     8701 gtgacgaaaa gaatcatgag atacgtgtta aacaaattga acggacgttg taacatctct
     8761 tgatgaagag gaacgtagga taattgaaat gaaggactta gggaatcgaa aggttaaaga
     8821 ttcgtttgtg tacaacgaat ttatgataaa gcgtgatccg ttttatcata aagaaaaaag
     8881 tgatattcag ttgatagcta cagctgtggg gattatataa acttagtttt gaggtatcgg
     8941 aaatgggatt ctagtatttt gtaatgttgt atagattggt taagatgagg ttgttctgct
     9001 ttacatgtta tattatttcc ctgccctgcc aattggtagg gttttttcat tctattaaaa
     9061 ataataatag ctcgtccaaa ccttttaaat ctatcttaca tagtatgtag taataaattt
     9121 aaaaggggga aactagtatg tcttattcct actatgatca ttgtaaggat aacaaaaaga
     9181 aatatcatga ttgtcataaa agtagtaaga gttatgacaa atgtgataaa aaacatgatg
     9241 aaaagccttg ggttaatgta aaagtgaatt gttgttctga tgataaccaa tacaatcttg
     9301 taagagcgtc tgcttttaga gctgtgagca cagttaatca gattgtgcca gcaaatactt
     9361 ttgtaaaagt cttattccaa aatgaacaat ttgatttagc aaatgaatat aaccctgcaa
     9421 catccatttt tataccaaag acaaggggtg tttattctgt tattggaaca attgcgttca
     9481 ttcccaataa tctaaacgta aactatagag cgcgggtaga aattcgagtt aatggaaatc
     9541 cagcaattgc aatagataat gatttttttg gtccaataaa ctttgctaat gttgttgccg
     9601 tttcatcaat aattcaattg aatgctgggg atgtagtcga ggtttttgca caaagtagcg
     9661 tagctggagt tatctctact gtagaaaata gtacgcattt tgaagctgca agatttccgt
     9721 ctccaacaga ataagtgata tgaaatccat cagtaatctt atctaagtaa agtcactaat
     9781 ttatatttga cctgcataaa tgcagggctt tttttcgata aaaagggaac gtttccgggg
     9841 atttctcaaa acagaatcaa tattactcta tatctataca gttctttgaa agaagaatag
     9901 tgtggggata gcgtagcccc tttatcaaaa cgttaccgga gaacgggcaa gggcggtaag
     9961 aatccgcaag aaggacgaaa agaccaattg aatttatata gtcagccacg atgtgtgagt
    10021 gtcgtggctt ttgtgtttct tattgagcct ggtatatttg ctgggttgat tggtgatata
    10081 aacgtttgat atacaggtgc tttaattata tataggggat tgctcttttt cgtttgcccc
    10141 tttacatata ttgaagagtg taggacaggc tcactcgctt gtatctacct tatttcaata
    10201 ctcataagaa actctttatc acattaagag catctactgg cgcaggtgct ctctttttat
    10261 tgaacaaaat gggggagggt tgttataaat gatagggctt aatccactcc atgaatggtg
    10321 gaagatgagt agt
//
