LOCUS       NZ_CP036016            10288 bp    DNA     linear   CON 25-JUN-2024
DEFINITION  Bacillus toyonensis strain BPN45/4 plasmid p246.
ACCESSION   NZ_CP036016
VERSION     NZ_CP036016.1
KEYWORDS    RefSeq.
SOURCE      Bacillus toyonensis
  ORGANISM  Bacillus toyonensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 246340)
  AUTHORS   Fiedoruk,K., Drewnowska,J.M. and Swiecicka,I.
  TITLE     The dynamics of pangenome and proteome of selected Bacillus cereus
            sensu lato representatives from the primary and anthropologically
            changes environments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 246340)
  AUTHORS   Drewnowska,J.M., Fiedoruk,K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Department of Microbiology, University of
            Bialystok, 1J Ciolkowskiego, Bialystok 15-245, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 191.4x
            Sequencing Technology :: Illumina MiSeq and MiniIon Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018739605.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 04:09:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,850
            CDSs (total)                      :: 5,697
            Genes (coding)                    :: 5,477
            CDSs (with protein)               :: 5,477
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 220
            CDSs (without protein)            :: 220
            Pseudo Genes (ambiguous residues) :: 0 of 220
            Pseudo Genes (frameshifted)       :: 93 of 220
            Pseudo Genes (incomplete)         :: 130 of 220
            Pseudo Genes (internal stop)      :: 65 of 220
            Pseudo Genes (multiple problems)  :: 52 of 220
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:13:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 171063
            Orig. end                         :: 181351
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10288
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10288
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10288
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5288](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5288)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    19..34
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 23.27"
                     /tool="antismash"
     misc_feature    complement(20..33)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 21.19"
                     /tool="antismash"
     gene            72..1193
                     /gene="tnpB"
                     /locus_tag="EXW33_RS28560"
                     /old_locus_tag="EXW33_29330"
     CDS             72..1193
                     /codon_start=1
                     /gene="tnpB"
                     /gene_functions="other (smcogs) SMCOG1192: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090861.1"
                     /locus_tag="EXW33_RS28560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_29330"
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_000973265.1"
                     /transl_table=11
                     /translation="MMINKAYKFRIYPNKAQATLINKTIGCSRFVFNHFLSLWDHAYKE
                     TGKGLTYGTCSAKLPAIKKEFVWLKEVDSIAIQSSVRNLADAYTRFFKKQNSAPRFKSK
                     KNNVQSYTTKQTNENIAVVGNKIKLPKLGLVRFAKSREVQGRIVNATVRRNPSGRYFVS
                     LLVETEVQELPKTHSYIGMDLGLKDFAILSDGTIYKNPKFFRSLEEKLAKAQRVLSRRT
                     KGSSRWNKQRVKVARIHEYMTNARKDYLDKISTEIIKNHDVIGIEDLQVSNMLKNHKLA
                     KAISEVSWSQFRTMLEYKAKWYGKQIIVVSKTFASSQLCSCCGYQNKDVKNLNLRKWDC
                     PSCRTHHDRDINASINLKNEAIRLLTARTVEIA"
     gene            complement(1972..2598)
                     /locus_tag="EXW33_RS28565"
                     /old_locus_tag="EXW33_29335"
     CDS             complement(1972..2598)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402086.1"
                     /locus_tag="EXW33_RS28565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_29335"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000402086.1"
                     /transl_table=11
                     /translation="MEIELKNISKKYENKQVLDNFNLVVNKGEMMAITGQSGTGKSTLL
                     NIVGLLEEPDSGDVIIQGIENAWKSEKKQIELFRYTIGYLFQNYALIDNETVSKNLDVA
                     LEYVKLSNKDDKKKEVLEKVGLLDKLNSKIYQLSGGEQQRIALARLMLKKNDIILADEP
                     TGSLDEVNRDQVLSILKSLNNEGKTILIVTHDPEVSKICTNVVTL"
     gene            complement(2604..4823)
                     /locus_tag="EXW33_RS28570"
                     /old_locus_tag="EXW33_29340"
     CDS             complement(2604..4823)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002154969.1"
                     /locus_tag="EXW33_RS28570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_29340"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_000722049.1"
                     /transl_table=11
                     /translation="MKKILITLVLVAYAVSSFVFTKHFEKREMVNINFSDRNGIQVVEL
                     NKDERPEDKLRKIEELSKKEKINIYKLSYKPIKTSKKQKVIIYAAIGDETQFKQKYPLE
                     WGKIFNQDHSVASYLSSSDTKDKEQVGKINIFSPELMVEIKPIEAAKDENLRGAYFVDS
                     NNPNIVQKVKEKLNTEIGFKTEESERAGAMNILENQGVDKYFIIVLTIVLLLIFLSFLY
                     YILLNYKSLAIRKLFGYTDRELIFKHLLKESIQIHTLSLMVVILGQVIYLFFYNGMSKF
                     LDFGSTWIVWQLIFSGISILVSLLPSLMIYHIKILELLKNKKPLKLVQFLNYSSKFIFS
                     IFLIIFSFNLINYYQDLSVQNSNQEQWDTTKNYSFYEYQSNIIGDRTNWEYETGIKSKK
                     LFGITNQKGALLIKPSDGITYKKQLEYNNSLNNTIGDVKEYDPQGGNTLQVNSNYLHDN
                     PVYDLNGKKINIDADYGDYLVILVPNKYRSNEADILKMYKEWYQFTRFIDEDKHKESIG
                     EKIEPHKQVEIKLTYIQDNQKHFLYNPMLEVKNQNYSKGAILIVVNSENMGGDSYLSYL
                     TGRYFFPKVDNPSQPYKELKEDIKIAGLQDIILTTPSLYSIVDDYLFKLKSELQFTLLF
                     VTLLLSIEIIITCFTILNYLERNKFIHAVQRINGFDFYKRHSKICFLLSLFWLIIYLGA
                     LTFKLATYTIISLIIPTYWLVELLIVYFIIRFVEKKKNKDVLKGA"
     misc_feature    4776..4796
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 26.98"
                     /tool="antismash"
     gene            complement(5001..5288)
                     /locus_tag="EXW33_RS28575"
                     /old_locus_tag="EXW33_29345"
     CDS             complement(5001..5288)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768886.1"
                     /locus_tag="EXW33_RS28575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_29345"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000768886.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 3.5e-17,
                     bitscore: 56.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLKKTVSSLVLSAAILGSGATAAFAATQNVGGGTWNYGTGGGSV
                     WSNYYHGSKVHKSSVIGQYYYSSPWTNPGYTSYASAPDTAGVDESYWSVK"
     misc_feature    5539..5552
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.97"
                     /tool="antismash"
     gene            5941..6075
                     /locus_tag="EXW33_RS29330"
     CDS             5941..6075
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653179.1"
                     /locus_tag="EXW33_RS29330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000653179.1"
                     /transl_table=11
                     /translation="MKDIYELLQEIHINLTEFEERKCSEVEKEKVKGFVKELIKKKED"
     gene            complement(<6227..>6335)
                     /locus_tag="EXW33_RS29190"
                     /pseudo=""
     CDS             complement(<6227..6335)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259899.1"
                     /locus_tag="EXW33_RS29190"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="DDE-type integrase/transposase/recombinase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MS"
     gene            complement(6378..6665)
                     /locus_tag="EXW33_RS28580"
                     /old_locus_tag="EXW33_29350"
     CDS             complement(6378..6665)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1058: ArsR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016107136.1"
                     /locus_tag="EXW33_RS28580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_29350"
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000935902.1"
                     /transl_table=11
                     /translation="MLNYEKDAEVFKALAHPVRIEILKELIIRGACNVTQIVGILGIPQ
                     STVSQHLAKMKSQKLIRNDRKGLEVYYSAQNKTINSIVEILLDYKSTNCK"
     misc_feature    complement(7028..7042)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.82"
                     /tool="antismash"
     gene            complement(7187..7498)
                     /locus_tag="EXW33_RS28585"
                     /old_locus_tag="EXW33_29355"
     CDS             complement(7187..7498)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637520.1"
                     /locus_tag="EXW33_RS28585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_29355"
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="WP_000637486.1"
                     /transl_table=11
                     /translation="MIVTTTNTIQGKEIIEYIDIVNGEAIMGANIVRDIFASVRDVVGG
                     RSGAYESKLKEARDIAMEEMKQLATQKGANAIVGIDVDYEVVRDGMLMVAVSGTAVRV"
     gene            complement(7762..8457)
                     /locus_tag="EXW33_RS28590"
                     /old_locus_tag="EXW33_29360"
     CDS             complement(7762..8457)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207761.1"
                     /locus_tag="EXW33_RS28590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_29360"
                     /product="response regulator transcription factor"
                     /protein_id="WP_000207755.1"
                     /transl_table=11
                     /translation="MTTILVLEDDITIRSFITLNMKRAGFNVLETDTGEKALELLEEHN
                     VDIVILDVMLPGIDGFRVCKSIRKNNEDIGIIILTGLVQEKDQVHGLTIGADDYIKKPF
                     SIIELVARVQSLLRRIQKNKKDLELIHSGPFQLNLLLGELYRNEIVIDLTPTEHIILRY
                     LMENSMKPISRNELLNKIWGINCVGNTKVIDVNINRLRQKIETSPSNPEFLLTVRGKGY
                     RWNENKHCN"
     gene            complement(9024..9305)
                     /locus_tag="EXW33_RS28595"
                     /old_locus_tag="EXW33_29370"
     CDS             complement(9024..9305)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053163.1"
                     /locus_tag="EXW33_RS28595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_29370"
                     /product="hypothetical protein"
                     /protein_id="WP_000053166.1"
                     /transl_table=11
                     /translation="MSLITPHYLLVGGCSKDKVLQAHKKAKEIFNQNNEINKLISPLKS
                     DSFFILYDGSHHRWKNEDEYMKAKTAYIQYLVESDIQFVEMATQELIS"
     gene            complement(9558..9680)
                     /locus_tag="EXW33_RS29335"
     CDS             complement(9558..9680)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562068.1"
                     /locus_tag="EXW33_RS29335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000562068.1"
                     /transl_table=11
                     /translation="MIASNGIIDIHSKKQAQEFTMEMTKQMLHETVGFKWGRNA"
     gene            complement(9711..9866)
                     /locus_tag="EXW33_RS28600"
     CDS             complement(9711..9866)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056873.1"
                     /locus_tag="EXW33_RS28600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000056873.1"
                     /transl_table=11
                     /translation="MSLPTQKKMLQRVKKEATSYCNVDPLAFAIGIRNGFLMVMPFWLV
                     VLYLVI"
ORIGIN
        1 ctgttacttt actacctatc gaatgtatgt ttgttgtatg atagacacat attgaaatgg
       61 gggtgaaacg aatgatgatc aataaagcat ataaatttcg tatctatcca aataaagcac
      121 aagcgaccct aatcaataaa acgattggct gttctcgttt tgtattcaat cacttcctat
      181 ctctatggga tcatgcatac aaagaaacag gaaaaggttt gacatatggt acatgctctg
      241 ccaaacttcc tgccataaag aaagagtttg tttggctaaa agaagtggat agtattgcga
      301 ttcagtcatc tgtccgcaac cttgcggatg cttatacacg ctttttcaaa aaacaaaaca
      361 gtgccccgcg tttcaaatct aagaaaaata acgtgcaatc ttataccaca aaacaaacaa
      421 atgaaaacat tgccgttgta ggaaacaaaa ttaaattgcc aaaactaggt cttgttcgat
      481 ttgccaaaag tcgtgaagta cagggacgta ttgtaaatgc tacagttaga cggaaccctt
      541 ctggcagata ctttgtgtca ttgttagtcg aaacagaagt gcaagaactt ccgaaaacac
      601 attcttacat tggaatggat ttgggactaa aagatttcgc cattttgtca gatggaacaa
      661 tctataaaaa tccgaagttt tttcgatcat tagaagagaa gttggcaaaa gcacagcgtg
      721 ttctttctag aagaacgaaa ggatcttctc gctggaataa acaacgagta aaagtagcta
      781 gaattcatga atacatgacg aatgctagaa aagattactt ggacaaaatc tcgactgaaa
      841 tcatcaaaaa ccacgatgtt atcggtatag aggatttgca agtatcgaat atgttaaaga
      901 atcataagtt agcaaaagca attagtgagg tatcatggtc acagtttcga actatgttgg
      961 aatataaggc aaaatggtac ggcaaacaaa tcattgtcgt atcgaaaaca tttgcttcaa
     1021 gccaattatg ttcttgttgt ggatatcaaa acaaagacgt taaaaatcta aacctacgta
     1081 aatgggactg cccttcttgt cgtacacacc atgataggga tattaacgca agtatcaatc
     1141 taaagaatga agcgataagg cttctaaccg caaggactgt ggagatagcc taataaatta
     1201 gagttcgata gaactcttta cttaggaatc cccctcttct aaacgaagtg aaagtggggg
     1261 tagttcaatg tttattttca ctaattctcc ataattgaat cttagccaaa ttaatattcc
     1321 tataacctat ccctagaaac ctctataaaa ttaaatggtt taagtacaga agaaataggt
     1381 acattacttc taatatgaat tagaaactga aatttgtttg tttattatcc atatgtacga
     1441 attccattgc tatcaagcta atattttgaa gcatccccaa aacaaaaaaa ttgtatattt
     1501 ttatgtggag atgccaattt tctcgttttg gggtatttac tttcttaagt tgatgggcat
     1561 gtggttttag gcttatcacg aaattgcgtc aattcaaata gttgcacaaa aacgaaacct
     1621 ttctgactgg gaattaaagg acatctaccg gaaaaggcta aagcctcagt taaaatgaag
     1681 ttgaatactg gagaataaag ccactaaaaa aaagaatgcc ctttttgtta tttaaaagaa
     1741 cattcttttt tataagataa gcgtacaaag gtttaaagaa tttcttgtta tgagagttag
     1801 tttttggatc tttatgtttc tcatctatgg cgaaagcctg ttacaataga actaagtcaa
     1861 atatgaaata cccttaggca gctctcatag gtataaaatc tatgggggat ttttcattac
     1921 agttattttg aaatttttgt accataaccg ttaatatctt attttcaaaa tttatagtgt
     1981 aacaacgttc gtacatattt tagacacttc agggtcatga gtaacaatta gaattgtttt
     2041 tccttcgtta tttaatgact ttaatatact aagcacctga tcccgattca cttcatctaa
     2101 tgatccagtt ggctcatctg ctaggatgat atcattcttt tttaacatga gcctagctaa
     2161 tgctattctt tgttgttctc caccactaag ttgataaatt ttactgttta atttgtctag
     2221 tagcccaact ttttctagaa cttctttttt cttgtcatct ttattagata acttaacata
     2281 ttctagtgct acatctaaat ttttgctcac agtctcatta tcaattaatg cataattttg
     2341 aaagagatag cctattgtat atctaaaaag ctcgatttgc tttttctcgc tcttccaagc
     2401 attttctatt ccttgtatga ttacatctcc agagtctggt tcttctaaaa gacctactat
     2461 atttaatagc gttgattttc cggttccact ttgacccgta atagccatca tttctccttt
     2521 atttacaacc aaattaaaat tatcaagcac ttgtttattt tcatatttct tagatatatt
     2581 ttttagctca atttccactt gaactacgct ccttttaata cgtccttatt tttcttcttt
     2641 tctacaaatc gaatgatgaa ataaacaatt aataattcaa ctaaccaata agttggaatt
     2701 ataagtgaaa taattgtata agttgctaat ttaaatgtta atgcacctaa ataaattatt
     2761 aaccaaaaaa gacttaagag aaaacagatt ttactgtgtc gcttataaaa atcaaagcca
     2821 ttgattcttt gaacggcatg aataaattta tttctttcta aataatttaa aattgtaaaa
     2881 caagtaataa taatctctat gctaagtaat aatgttacaa aaagaagagt aaattgaagt
     2941 tcgcttttta atttaaagag ataatcatct acaatactat ataatgacgg cgttgtaaga
     3001 atgatatctt gaagacctgc gattttaatg tcttctttta attctttata aggctggctt
     3061 ggattatcta cttttgggaa aaaataacgc ccagttaaat aacttaaata gctgtctcca
     3121 cccatatttt ctgagttaac aacaatcaat atagcgccct tgctataatt ttggttttta
     3181 acctctagca taggattata caaaaaatgt ttttggttat cttggatata ggtaagttta
     3241 atttcaacct gtttatgtgg ttcaattttt tctcctatgc tttctttatg tttatcttca
     3301 tcaataaacc ttgtgaattg ataccattct ttatacattt ttaaaatatc agcttcgttg
     3361 ctgcgatatt tattgggaac taatataact aaataatcac cataatcagc atcgatattg
     3421 atttttttac cattcaaatc ataaactgga ttatcatgta aataattaga attcacttga
     3481 agggtgttac cgccttgagg atcatactct ttaacatctc ctatagtatt gtttaaagaa
     3541 ttgttatact ctaactgttt tttgtacgtt ataccatctg atggttttat aagcaaagcc
     3601 cctttttggt tagttattcc aaatagtttc ttgcttttaa ttccagtttc atattcccag
     3661 tttgtgcggt cgcctataat attactttga tattcataaa aactataatt ttttgttgta
     3721 tcccattgtt cctggttaga gttttgtaca gatagatctt gataataatt tataagatta
     3781 aaagaaaaga taattagaaa aatcgaaaag ataaactttg aactataatt taaaaattga
     3841 actagtttta atggtttttt attttttaat agttccaaaa tttttatatg atatatcatt
     3901 aaagaaggaa gtaggctaac tagaatagaa atacctgaaa aaattaattg ccacacaatc
     3961 catgtactac caaaatctaa gaatttactc atcccattat aaaaaaacaa gtatattact
     4021 tgtcctagaa taactaccat taaggataac gtatgaattt gaatactttc ttttaataga
     4081 tgcttaaaaa tcagttctct atccgtatat ccaaacagtt ttctaattgc caaactttta
     4141 taatttaata atatataata taaaaaactc aggaagatga gtaacagaac tatagtaaga
     4201 actataataa agtacttatc aactccctga ttttctagga tgttcatagc acctgctcgt
     4261 tctgactctt ccgttttgaa cccgatttcg gtatttaatt tctcttttac cttttgaaca
     4321 atgtttggat tgttactatc aacaaaataa gcgcctctca agttttcatc ttttgccgct
     4381 tctataggtt taatttctac cataagttca ggtgaaaata tgtttatctt tccaacctgc
     4441 tctttatctt tcgtatcgct tgatgacaaa taacttgcta cggaatgatc ttgattgaat
     4501 atctttcccc attctagtgg atatttttgt ttgaattgtg tttcatcccc tatagctgca
     4561 tagataatta ctttttgttt ttttgaagtt ttaatcggtt tatatgataa tttatagatg
     4621 ttgatttttt cctttttgga taattcctca atctttctca gtttatcttc cggacgttca
     4681 tctttattca attcaaccac ttgaattcca tttctatcag aaaaattaat atttaccatt
     4741 tctctttttt caaagtgctt agtaaataca aatgaagata cagcataggc tacaagtact
     4801 aaagtaatta atattttttt cataccagtc tccattttcc gtattctatt ttcattttga
     4861 attcaaatga aggtagaata tcctttgtct tcaaatatta ataaaaataa tttacttatc
     4921 tatatgtata taagtaacat aaaatagaat cctagaacat gcaggtaata acccacgagt
     4981 tctaggattt ttcttacttt ttatttaaca gaccaataag attcatctac accagctgta
     5041 tcaggtgcac ttgcatatga agtgtaacct gggttcgtcc atggagaact ataataatat
     5101 tgtccgatta ctgaagattt gtgaactttg cttccgtggt aataatttga ccaaactgaa
     5161 ccaccaccgg ttccataatt ccatgttccc ccccctacat tttgtgttgc agcaaatgct
     5221 gctgttgctc ctgaaccaag tattgcagca cttaatacta atgaacttac agtttttttc
     5281 aatttcaatt tttttccccc tttaacatct aattaattta tttagatatt gcagactgac
     5341 taccttttta tttttaaaca accgcattaa aaaataaata tatagaacag aatacaataa
     5401 actaacaaat aggaagtaaa taaggtaaag atactcaaat gaaaaaataa ttcaattaaa
     5461 aatagggtgg gaaatattaa cttcaaatcg ttttttttaa tattatcaca gtttttttca
     5521 agaaagacat attcaaaatt ttttaaataa aattctaaat tttcagtatt tatttgatat
     5581 aaaaatagtg atacttaaaa ataaaatttt caatcaagta aatatttaca aatatagagg
     5641 ggagtaattg gaaagtgttt taaataatac aatttaaaaa atagacattt tcaatttttt
     5701 gttaccatgg aatcgtatgg catgaaatct aaaaaaaatt tgtggtaagt gtgagaaatc
     5761 actttgttta atacgttata taaggtataa gaaattgtct atccattatt aacgttacat
     5821 attacaaatg aaatatttct ataatatgta aataataaat ttgtactaaa tacgccttag
     5881 attcactttc ttaattgcat aaaaaattaa aataagaaag aatttaggag gtgggatagt
     5941 atgaaagata tttatgaact attacaagag atccatataa acctaactga atttgaagaa
     6001 agaaaatgta gtgaggtaga aaaagagaaa gtaaaaggtt ttgtaaaaga gttaattaaa
     6061 aagaaggaag actgaaaatg tttataacgc aagtatcaat ctaaagaatg aagcgataag
     6121 gcttctaacc gcaaggactg tggagatagc ctaactcttt acttaggaat ccccctcttc
     6181 taaaagaagt gaaagtgggg gtagtttaat cataaggagt cattgacgta tttgaaacat
     6241 gaaaattaat tgtattaact ttaaataaca gcacggtatt agtacatcca ttgaccttta
     6301 atttttacat atgtctcatc aatccgctaa gaatccttga ttgatgtagg gggttctgga
     6361 acaaagatta ttatttttta cttacaatta gtagatttgt aatctagtag tatttcaaca
     6421 atggaattaa tagttttgtt ttgggcacta tagtagactt ctaatccttt tctatcattt
     6481 ctaattaatt tttgagattt catctttgct aaatgctgtg aaacagtaga ttgtggaata
     6541 cccagtatac ctacaatttg tgttacatta catgcccctc ttataattaa ttctttcaaa
     6601 atttcaattc ttactggatg agctaatgcc ttaaaaactt ctgcgtcttt ttcataattt
     6661 aacaaactaa acatctcctt ttgaatcgat atatgtttat aatattaata atttattctg
     6721 catcatcttt ttgatttaat tagtttttta ttttttcaac ataccctggt atgcttgaga
     6781 cactcttatt ctcttttttt ttacttcctt taattcatct gaaaatgttg catttggctt
     6841 tttcatataa gacataattc caagatcatc tttgatggtt tcttacctaa gtaactttgc
     6901 ttaaccgaat cagtggtttc tagtagtagg aattgactcc cttcatgtac tttcgcaaga
     6961 atcctttttg ggatatgcca gaaattccaa tagttaatag cgattgtgta aactctacac
     7021 ctaatgtatc gacttatttg atacattgtt agctattcgc cagaacctct atgtaactaa
     7081 tttttttcaa agagtatttt tctataaaaa actgcaactc taattgttag ttatgtctaa
     7141 caattagaat tgcagtgcac caattaggtt atattttgct ttctatttag acacgtacag
     7201 ctgtaccgct tactgcaacc attagcattc catcacgaac gacttcgtag tctacatcga
     7261 taccaacaat tgcatttgct cctttttgcg ttgcaagttg tttcatttct tccatggcaa
     7321 tgtcacgagc ttcttttaat ttactttcat aagcaccaga acggccacct acaacgtcac
     7381 gaacagaagc gaagatgtcg cgtacgatat ttgcacccat aattgcttca ccatttacga
     7441 tgtctatata ctcaataatt tctttaccct gaatagtatt agttgttgtt acgatcatta
     7501 ttaacacact cctataattt ttaaaaagtt tttgttgatt gctataatcc tattattaaa
     7561 tttaaaagct aaattaacta tctaataacg ttacataaag atgacagttc tgtaagggtg
     7621 aaagaaaaca agctatccac aaaaaacttg ttttcttaac actaatatta aaatgctttc
     7681 aatagtaaaa atagtttttg taagttttgt aaatactttt gtaattaaaa aaaagtataa
     7741 gcgaatcgat tttctgccaa attagttgca atgtttgttt tcgttccatc tatatccttt
     7801 tcctcgtacc gtaaggagaa actcaggatt agaaggacta gtttcgattt tctgacgaag
     7861 acgattgata ttcacatcga tgacttttgt attacctaca caatttatac cccatatttt
     7921 atttaagagt tcattacgtg aaattggttt catggaattt tccattaaat atcgtaaaat
     7981 aatatgctct gttggagtta aatcaattac tatctcattc ctatataatt ctcctagcag
     8041 aagatttaat tgaaatggcc ccgaatgaat caattccaaa tcctttttat ttttttgaat
     8101 tcttcttagc aatgattgta cacgtgctac taattcaatt atactaaatg gctttttaat
     8161 ataatcatct gccccaattg tcaaaccgtg tacctgatct ttctcttgaa caagtcctgt
     8221 taatataata ataccaatat cttcattgtt ttttcgtatg cttttgcata cccgaaatcc
     8281 atctattcct ggtaacataa catcaagtat tacaatatca acattatgct cttctaaaag
     8341 ctcaagagct ttttcacctg tatcggtttc taaaacattg aaaccagcac gcttcatatt
     8401 caatgtaata aaactacgga tggttatatc atcttctaat actaaaattg ttgtcatact
     8461 caccgctcct ttactcttgt tgataaacgg ctatagattt gatataagtt ctaagattct
     8521 agtagtttat ttttaaaaaa ttgtatatac atgagctctt ttttatatgt atcaattcct
     8581 aggagtcact atatatgtcc atcaacttaa aaatctagta aaaaactgtc atcccttata
     8641 tttagccttt tagaaagaat aacgtggaac tagtaaagat tcttaagttg gtggacatgg
     8701 agtggtatcc ctagtttatt cgccctatat taattaggtg ataagacttt ttcgtcctta
     8761 atctcattct cctgtcaact ctaccaccac ataatttatt acgtgaaaat gaaatatttt
     8821 tcatcgtttg ctcttaaatt cccaggtttt tatatacacc tcaaaacgct ttgactagat
     8881 aatacaagct ctcttctgat tcacctgaat aaagttgagc ctcttgaaac tccccaatcg
     8941 attagctgat taatgatgcg ccgttttttt ctttctacat gatagatgtg ctgttctagt
     9001 ttttttggac cttttgcatg ctttcaagaa attaattctt gtgttgccat ttctacaaac
     9061 tgaatatcag actctacaag gtattgaata tatgccgttt tcgccttcat atattcatct
     9121 tcatttttcc atctgtgatg gctgccatca taaagtataa aaaaggagtc gcttttcaac
     9181 ggacttatca atttatttat ttcgttattc tgattaaata tttcttttgc ttttttatgt
     9241 gcctgtaaca ctttgtcttt cgaacatcca cctacaagca aataatgtgg agtaattaaa
     9301 ctcattttgt tattcccccc tctattgccc tcatatacga tccagaaagg ataggaacaa
     9361 agagcacgta ttgattcttc aagctaacat gatttaaaaa gcatgaatga gaggaaggat
     9421 aatctcaaat ggctagtgat atcttctaaa aagctgtcct tcttctcact ttgtacaaca
     9481 aacactttaa gttcggataa atgagcttgt tctttatact tagctttaat atcaaagatg
     9541 gttccactta aaaatgatta tgcatttctc ccccacttga atccaacggt ttcatgaagc
     9601 atttgttttg tcatttccat tgtgaattct tgggcctgct ttttcgaatg gatatctata
     9661 attccattcg aagcaatcat ttctcccacc ctttcgtagt tatggcgttt ttatataact
     9721 aaatacaata caactaacca aaatggcatt accattaaaa atccatttct aatccctatt
     9781 gcaaaagcta aaggatcaac attacagtat gacgttgcct cttttttaac ccgctgcaac
     9841 atcttttttt gggtgggaag gctcatagtg aatctcctta tctttcaatc tgttttcgtt
     9901 ttccataaca aactaccacc aaaacagcct atacaataaa tcacaatgaa tttcataaaa
     9961 atttctataa ttgcatccgt cattccccat tctcctctcc caaggcctgt aatacacaag
    10021 ctataattag caaagtaaga attaataata agctccctat atttcggcat tttctaagca
    10081 tcggcattct ctcttcataa ttaacgttta ttttagaaaa aaaccaagct aagaatatta
    10141 agagaagaaa agagcccaat ttacctcgtt atgagcaaat cggctcttta atattcacat
    10201 gacataagcg atgtaaactt attgggtgta atctcccaaa cttatattct cctaagaaat
    10261 taatatcttc ctagcctaaa agcgaata
//
