LOCUS       NZ_CP036014            10324 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Bacillus toyonensis strain BPN45/4 chromosome, complete genome.
ACCESSION   NZ_CP036014
VERSION     NZ_CP036014.1
KEYWORDS    RefSeq.
SOURCE      Bacillus toyonensis
  ORGANISM  Bacillus toyonensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5446351)
  AUTHORS   Fiedoruk,K., Drewnowska,J.M. and Swiecicka,I.
  TITLE     The dynamics of pangenome and proteome of selected Bacillus cereus
            sensu lato representatives from the primary and anthropologically
            changes environments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5446351)
  AUTHORS   Drewnowska,J.M., Fiedoruk,K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Department of Microbiology, University of
            Bialystok, 1J Ciolkowskiego, Bialystok 15-245, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 191.4x
            Sequencing Technology :: Illumina MiSeq and MiniIon Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018739605.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 04:09:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,850
            CDSs (total)                      :: 5,697
            Genes (coding)                    :: 5,477
            CDSs (with protein)               :: 5,477
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 220
            CDSs (without protein)            :: 220
            Pseudo Genes (ambiguous residues) :: 0 of 220
            Pseudo Genes (frameshifted)       :: 93 of 220
            Pseudo Genes (incomplete)         :: 130 of 220
            Pseudo Genes (internal stop)      :: 65 of 220
            Pseudo Genes (multiple problems)  :: 52 of 220
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:13:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4898773
            Orig. end                         :: 4909097
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10324
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10324
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10324
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5324](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5324
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(59..72)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.92"
                     /tool="antismash"
     gene            989..2437
                     /locus_tag="EXW33_RS24850"
                     /old_locus_tag="EXW33_25465"
     CDS             989..2437
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-465). E-value:
                     1.2e-29. Score: 95.4. Matches aSDomain:
                     nrpspksdomains_EXW33_RS24850_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="EXW33_RS24850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_25465"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_000471599.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.2e-27, bitscore:
                     95.3, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYFKKMIMKGHLLGDQKIPSTRELSEL
                     LSVSRNTVLSAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTTLADE
                     LDLMKHGVRWEKGMVVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYRPL
                     MKYLLHYMEIKGVDISNKDILITNGFTEGLDIVLSSLAKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMNDDGIDTTEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYKVPIIEDGFNEELRYSGSHLSPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQY
                     IPFKRMTGDGGLHLFIELEEKIHARTLLQKCYEKGVTFSPGDVFYSDGGGANTFRLGFS
                     RLKEKEIVSGIQIIGETLKNETWS"
     aSDomain        1424..2383
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EXW33_RS24850_Aminotran_1_2.1"
                     /evalue="1.20E-29"
                     /label="EXW33_RS24850_Aminotran_1_2.1"
                     /locus_tag="EXW33_RS24850"
                     /protein_end="465"
                     /protein_start="145"
                     /score="95.4"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYRPLMKYLLHYMEIKGVDISNKDILITNGFT
                     EGLDIVLSSLAKKSGRVICENPTHHAALKLFRLHGLEVHGIDMNDDGIDTTEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYKVPIIEDGFNEELRYSGSHLSPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQYIPFKRMTGDGGLHLFIELEEKIHARTL
                     LQKCYEKGVTFSPGDVFYSDGGGANTFRLGFSRLKEKEI"
     gene            2442..3356
                     /gene="ddlB"
                     /locus_tag="EXW33_RS24855"
                     /old_locus_tag="EXW33_25470"
     CDS             2442..3356
                     /EC_number="6.3.2.4"
                     /codon_start=1
                     /gene="ddlB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="EXW33_RS24855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_25470"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_002038752.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 1.2e-21,
                     bitscore: 75.6, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 2.6e-13, bitscore:
                     48.6, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 4.4e-08, bitscore: 31.3,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 3e-51, bitscore:
                     172.1, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 7.1e-06, bitscore:
                     24.0, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSGICMDKNISKKILRY
                     EGVETPDWIELTKMADLKLDDLDKLGFPLVVKPNSGGSSVGVKIVNDKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSIFDGKHLPIVSIRHAAEFFDYNAKYDDVSTIEEVIELPT
                     EIKERVNKASLACYKALKCSVYARVDMMVKDGIPYVMEVNTLPGMTQSSLLPKSADAAG
                     INYSKLLDMIIETSLRVRKEEGF"
     regulatory      3430..3535
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            3662..4351
                     /gene="tenA"
                     /locus_tag="EXW33_RS24860"
                     /old_locus_tag="EXW33_25475"
     CDS             3662..4351
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="EXW33_RS24860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_25475"
                     /product="thiaminase II"
                     /protein_id="WP_000144532.1"
                     /transl_table=11
                     /translation="MTFSQTLRKEVDSIWEASFHHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITEEEKGHFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYKEWISAY
                     GSDWFRTLVEEQITRLDTIAEQVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDT
                     EVKDVIG"
     gene            5001..5324
                     /locus_tag="EXW33_RS24865"
                     /old_locus_tag="EXW33_25480"
     CDS             5001..5324
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108508.1"
                     /locus_tag="EXW33_RS24865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_25480"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001032074.1"
                     /sec_met_domain="TIGR03601 (E-value: 9.8e-37, bitscore:
                     123.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKFQQELQALSLNDYQSGNIVYWDQQNQYPYYYFEDAARRCGGC
                     GRCGGCGGRCGGGGFRCGGFRCIGCFGCFGCGGCGGCGGCSNCFNGFNGTTDTTGTIVT
                     YEY"
     gene            complement(5480..5752)
                     /locus_tag="EXW33_RS24870"
                     /old_locus_tag="EXW33_25485"
     CDS             complement(5480..5752)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088127.1"
                     /locus_tag="EXW33_RS24870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_25485"
                     /product="hypothetical protein"
                     /protein_id="WP_000088127.1"
                     /transl_table=11
                     /translation="MSRKSDITAKDRKDAGKKALKSAVVKGWINVGKWGFFGDMVAFMD
                     RYYTGYKNYNLKKKRERRADSSSSFWFWCNKSAYKSQRVRQEYKT"
     gene            complement(6190..6537)
                     /locus_tag="EXW33_RS24875"
                     /old_locus_tag="EXW33_25490"
     CDS             complement(6190..6537)
                     /GO_component="GO:0043592 - exosporium [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343262.1"
                     /locus_tag="EXW33_RS24875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_25490"
                     /product="exosporium leader peptide-containing protein"
                     /protein_id="WP_000343263.1"
                     /transl_table=11
                     /translation="MDEFLSSAALNQNSIGPTLPPMQPFQFPTRPTGNTGPPGIVSVTF
                     NQSHIPATSPTNLVLPAISVGVNQIVKLEGVFRSDYVSLSPLTEFTVSSILALKRGVFT
                     IAVQMELQEDI"
     misc_feature    6661..6677
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 22.69"
                     /tool="antismash"
     misc_feature    complement(6661..6677)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.06"
                     /tool="antismash"
     misc_feature    7498..7516
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.44"
                     /tool="antismash"
     gene            complement(7616..7915)
                     /locus_tag="EXW33_RS24880"
                     /old_locus_tag="EXW33_25495"
     CDS             complement(7616..7915)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110270.1"
                     /locus_tag="EXW33_RS24880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_25495"
                     /product="hypothetical protein"
                     /protein_id="WP_000110272.1"
                     /transl_table=11
                     /translation="MSVFINYAITPKVFNPNLTYLPNNRRSFIPTQKESSKNICYLLET
                     YYLGKKIKQLLVNGTILKASIFVGFHNRSRLATFIDENGDITVVNCERIDAILL"
     gene            complement(7996..8217)
                     /locus_tag="EXW33_RS24885"
                     /old_locus_tag="EXW33_25500"
     CDS             complement(7996..8217)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807684.1"
                     /locus_tag="EXW33_RS24885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_25500"
                     /product="hypothetical protein"
                     /protein_id="WP_000807684.1"
                     /transl_table=11
                     /translation="MAEHTKCVCEQLERLQLGTEVDVFLTGTTLEDLIFTNFNPDNFCV
                     TFVDNTTEPGSIIVLDCRDIQALRIEAE"
     gene            8706..9506
                     /locus_tag="EXW33_RS24890"
                     /old_locus_tag="EXW33_25505"
     CDS             8706..9506
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097507.1"
                     /locus_tag="EXW33_RS24890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW33_25505"
                     /product="C1 family peptidase"
                     /protein_id="WP_000097507.1"
                     /transl_table=11
                     /translation="MSSNESNFSFTSNKDIVKYLNVRKDPVDLRDKIFRSYLFSNEADL
                     PTQVDLRPKMSGIVNQWNIGSCTANAIVSGLREYLLLQSGRPFTCLSRLFLYYHEREIE
                     GHIQEDSGANVRDGMDVLKKIGVCPEEDFPYTENFRDKPSPKAESDATLYKIGTYHRIQ
                     DLSGLKAALAHGLVVVLGFAVPKSFMSEEVKKTGIVPVSGTDDVIVEDGQQAGHAVCAA
                     GYDDNKKTVIIRNSWGEEWGDKGYCYFPYELFENGIVQDMWTGR"
     gene            9789..9950
                     /locus_tag="EXW33_RS24895"
     CDS             9789..9950
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866154.1"
                     /locus_tag="EXW33_RS24895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000866207.1"
                     /transl_table=11
                     /translation="MKVLKNQLREWKNQSKKKNKKKRKEKFSTREIEDLMGVHRPCYER
                     RRGALRQK"
ORIGIN
        1 tattggtaga atcaacagtt acaaatctta aagatggaaa tccgggaaaa aaatatataa
       61 aacaagcgat tgagaaacat atggatattg ttgcaatttc aaaaggtgca ctcgttacta
      121 actggagaga aataaaggca gcagcaaaag aagcgaatgt acgaattcgc tatagtggcg
      181 caactgctgc agcactaccg acattagata ttggacaatt tagtttggct ggatgcaata
      241 ttgaaaaaat agaaggaata ttaaacggga caactaatta tattcttaca aaaatgtatg
      301 aagaagatgt tacgtttgaa gaagcactga aagaagcgca aaataaagga attgctgaga
      361 cgaacccaat attagatgta agtggttcgg atagtgcgtg taaattatta cttttggcaa
      421 acagcttgat gggtgttgag aatatgctta ctgatatgca tataaaagga atcgaaaatg
      481 ttacaaaaca acaaattcga catgctaagg aacaaaacaa ttatattaaa ttaatagctt
      541 cagcatataa ggatatggat ggtaaagtga atcttaatgt agaaccgctt gaaatacaaa
      601 aagaccatcc actagcaaaa gtaaatggaa ctgaaaaagg aataacattc tttacagata
      661 caatgggaca agttactaca attggtggag cttctaatcc acgcggagcc gcagcagctg
      721 ctttaaaaga tgtaattaac ttatatcgta gagatttata gaagtaaaac tcacaccttc
      781 aaaattcgga tggagaaaaa aagttaggtg ggagcctttt agcccatata tgtccgattc
      841 gggctaaaaa tcggtggggg atgaataaaa ttcccacgga ttaaagtttc actttataag
      901 accgtaccgc tgttaataaa aaatacatat gtagtggacc ttttgagaat gaaataaata
      961 tgcaaaaata attgcagggg gtaagaaatt gtttaaagat tttaaagttg taaaagatcg
     1021 tcccgtttat attcaattaa aggattattt taaaaagatg attatgaaag ggcatttgct
     1081 cggtgatcaa aagatcccat caacaaggga actgagtgaa ttattatcag tgagcagaaa
     1141 tacagtactc tctgcttatg cggatttaga acaagaagga ctcatttatg cagtaaaagg
     1201 aaaagggaat ttcgttgcga aggtggatat atctaacact tcgtctgttg aaatagattg
     1261 gaaaaataaa cttaatacag ttaccacatt agcggatgaa ttagacttaa tgaagcatgg
     1321 agtccgctgg gaaaaaggaa tggttgtttt taatagtata gccccggacg aaaagctatt
     1381 tgatgttgaa aatttcaaaa gagcttttct tactcgtatg tccattgaag gggatatcgt
     1441 attgaactac ggatatgcaa aaggttaccg cccgttaatg aaatatctac ttcattacat
     1501 ggaaataaaa ggtgtagata tttcgaacaa agatatttta attacaaatg gatttacaga
     1561 aggattagat attgtactat cttccttagc gaaaaaatca gggcgtgtga tttgtgagaa
     1621 tccaactcac catgctgcat tgaaactttt tcgcttacat ggacttgaag ttcatgggat
     1681 agatatgaat gatgatggta ttgatacgac tgaagtagaa aaaagtttac gtgaaaagga
     1741 ttttgatttt gcgtatttaa ttccttctta tcataatcca actggtattg ttacaagttc
     1801 ggagaaaaga acggaattga tgaggttatt ttcgaaatat aaggttccta ttatagagga
     1861 cggatttaat gaagaattac gttattcagg ttcacattta tcgccactat taacttttgc
     1921 aggggctggt aataatgtga tttatattag tagcttttcg aaggtacttt tccctggttt
     1981 acgtgtaggg tggattattg ccgataaaga actgattcat tatttagaaa gtgttaaaag
     2041 agcaagaacc attcacacat ctacgttaga tcaagctgtt ttgtttcaat atttacatga
     2101 aggatatttt gaaaaatatt taaaaaaggc aagatctgtt tacaagaaaa aatatgagtt
     2161 agccgttggg gcttgcaatc agtatattcc gtttaaaagg atgactggag atggcggact
     2221 tcatttgttt atagagttgg aagagaaaat tcacgctcgc acacttttac aaaagtgtta
     2281 tgagaaaggt gtaacgtttt ctcctggaga tgttttttat tctgatggag ggggagcgaa
     2341 tacattccgc ttaggttttt cacgtttgaa ggagaaagaa atagttagtg ggattcaaat
     2401 aattggtgaa actttaaaaa atgaaacttg gagttgagaa aatgagaatt ggcgttatta
     2461 tgggaggagt atcctctgaa aaacaagtat caattatgac tggaaatgaa atgattgcac
     2521 atttagataa aaataagtat gaaatcgttc caattacgtt aaatgaaaaa atggatttaa
     2581 ttgaaaaagc gaaagacatt gattttgcat tgctagcatt acacggaaaa tacggagaag
     2641 atggtacggt tcaagggacg cttgagagtt taggtattcc atatagtggc agcaatatgt
     2701 tatctagcgg tatatgtatg gataaaaata tttcgaaaaa gattttgcgt tatgaaggag
     2761 tagaaacacc agattggatt gaacttacaa aaatggcgga tctaaagctt gatgatttag
     2821 ataagttagg atttccgtta gtggtaaaac caaactctgg cggctcgagt gttggcgtaa
     2881 agatcgtcaa tgataaaaat gaattgatct caatgctgga aactgtattc gaatgggatt
     2941 ctgaagtagt aattgagaag tatataaaag gtgacgagat tacttgttca atttttgatg
     3001 gtaaacattt acctatcgtt tcaattcgac atgcagctga gttttttgac tacaatgcaa
     3061 aatacgatga tgttagtaca attgaagaag ttattgaact tccaactgaa attaaggaac
     3121 gtgtaaataa agcgtcattg gcttgttata aagcattaaa atgtagtgtt tatgcaaggg
     3181 ttgatatgat ggtgaaggac ggaattccat atgtaatgga ggttaataca ttaccaggga
     3241 tgacccaatc aagtttactg ccaaaaagtg cagatgccgc ggggattaat tatagtaaac
     3301 tattagatat gataattgaa acttcattaa gagtgaggaa agaagaaggt ttttaagata
     3361 attatacaat taaaactttt gatagttcca cgaagtatgc tatgataatt acaatacgac
     3421 aactaaatat ttatccacta ggggggcctt ttgtaggctg agatcaaaca taattttgag
     3481 actcttagta cctgatctgg ttaataccag cgtagggaag tgggaagacg attatattta
     3541 aagaatacat taatattgtc tatttcactt tctttacgcc tgtaaagaaa gtttttttgt
     3601 tttacagatt taataactgt ggataaatac atatatttta tacattattg gaggaattta
     3661 aatgactttt tcacaaacat tacgtaaaga agtagattct atttgggagg caagttttca
     3721 tcatcctttt gtaaaaaaac ttggtgaagg tacgttagat ttggcaagtt ttcgttatta
     3781 cgtacttcaa gattcatatt atttaagtca ttttgctaga gtacaaactt taggagctgc
     3841 aaaggctctt gaattagaaa cgacagctcg catggcacac catgctcaaa atacatacga
     3901 ggcagaatta tcattacatg agaatttcgc aaagaaatta ggaattacgg aagaagaaaa
     3961 aggtcatttt atccctgccc caactgcata tgcgtatact tcacatatgt accgtgcggc
     4021 gtatgaaggg catttagggg atattatcgc agcaatttta ccttgctact ggctatatta
     4081 tgaaattggt gaacgtttaa aagaatgtca gccagaagag ccgatttata aagagtggat
     4141 ttctgcttat ggatctgatt ggttccgtac tttagttgaa gagcaaatta cacgattaga
     4201 tactatcgct gaacaagtga cagagtctga tcggaagcgt atgaaacagc atttcattat
     4261 tagtagtcaa tatgaatatt cattttggga aatggcttat acattagaaa aatggccagt
     4321 ggatacagaa gtaaaagatg taattggata aagagtagag gtattgttat acatatgtat
     4381 ggataatacc tctttatatg atttcaaaaa aggggtatcg caaaaaagac gtatcaatct
     4441 aagtgcgtct tttttcaatt ttcttcaatt agcaatttca tggagagctt gctgaaaaag
     4501 tagaatgaaa agccctaatc agggcttttt ttgtaggaat ttcttaaccg tcattgagtc
     4561 ctgtcactag taggtgtttt gacgctacga cggactaaat cgattattcg gctagtgaag
     4621 tatataaaaa agttaggaat atctgaaggt gctctcagaa agtatatgga agtgttataa
     4681 cgagaggcat ttacagtaca gaaaaatcta agcggcatac gggaatacgt aggatacggc
     4741 atcatggtta ttgagaaatg aattgaactt agtaagcatg aagatatgac gcaagcattc
     4801 acattttata atttctattt ataaaaatga gcccatagta aaaataggta tatgaacaag
     4861 gtgcaccgtc tagtgatagg tcatacttta taggtgtacg tattgaactt agtttatagc
     4921 gttatttcta cttagattta acgaacagaa atattggaaa gcatcgatga ggttaaagtt
     4981 tataaacgga ggtcatgaaa atgaataagt ttcaacaaga actacaagcg ttaagcctta
     5041 atgattacca gtctggaaat attgtgtatt gggaccagca aaaccaatat ccatactact
     5101 attttgaaga tgctgctcgt cgctgtggcg gttgcggtcg ttgtggcggt tgtggaggtc
     5161 gttgtggtgg tggtggattc cgttgtggtg gattccgttg tattggttgc ttcggttgtt
     5221 ttggttgtgg cggctgtggc ggctgtggcg gttgttcaaa ttgttttaat ggttttaatg
     5281 gtaccactga tactactggt actattgtaa catatgaata ttgataagag agaaaaggaa
     5341 tctactttgt aaagcgttat acaattttat aaaagagata aatcaaaaca cacgtagcat
     5401 cttttaattt agagagataa tttatccaac cttttataaa cattgtgact ttatttcaaa
     5461 tcaaaaatcg aatgtctttt tatgttttat actcttgacg cactctttga cttttataag
     5521 cagatttatt gcaccagaac caaaaagaag agctgctatc agcccttctt tctcttttct
     5581 ttttaagatt gtaattctta tagcctgtat aatatctgtc cataaatgca accatgtcac
     5641 caaagaatcc ccatttacct acatttatcc aacctttaac aactgccgat tttaatgctt
     5701 ttttccccgc atctttacga tcttttgctg ttatatctga ttttctgctc attctcatac
     5761 tttatatgaa atatgtgaaa atcagtatga atggtggaag tgagcgcaac ttcaaaagct
     5821 gtttattggg attaacaaat ttaataatct atttttatta ctattgatta attttgaaac
     5881 gtaacaaaac tacgtaactg tttgtataaa atgcactgct ctagtattga aaatttagaa
     5941 catggaatag ggtgctttgt ttctacaagg tgtttttgtt ttacttcata ttacgattta
     6001 tagaccggga attacacttt aaatgtttat tactgtagca gttatgtttc tttcactaat
     6061 agtgttagtg gatgtaccaa ttcccgtggc attcagagat agcctgtata cataagtacc
     6121 agggggaggc gtatctatcc agactattga aacgttgtgt gttaaatttg ttgaaacatt
     6181 tactggtttt taaatatctt cttgaagctc catctgaacc gcaatagtaa agacgcctct
     6241 ttttagagcg agaattgaag atacagtaaa ttctgtaagg ggacttaggg aaacataatc
     6301 agatctgaaa actccttcta actttactat ttgattaacg cctacactaa tagctggtaa
     6361 tacgaggtta gttgggctag ttgcaggaat atgactttgg ttaaatgtaa ctgaaacaat
     6421 gccaggtggt ccggtgttac cggtcggtct agtagggaat tgaaaaggtt gcataggtgg
     6481 gagtgttggt ccaatcgaat tctggtttaa tgcagcagaa gataaaaact cgtccaatat
     6541 aattcacctc taaaaaattt atattactga tagtaaatgt aggcgttgag caaagtgaca
     6601 tggacaagcg gttgtatgtt aaaaattaaa caaaatagtt attttattaa agaaaaaaca
     6661 tgcacaaaaa ttgcgcatgt ataataaaaa gctatttctt gtctatgtaa atatatgatt
     6721 ggcttataca aaccgtgagt gtgtttgaga aaaatggtta tttattaaca aatataaaga
     6781 gcgcttttaa ggcgctcttt aaccaaaact aatattaaaa aagaataccg catgatattt
     6841 tatgtatgtt cttaataaat gtgcggtttt taaacaaaat ccttatttta tcttaaataa
     6901 aaagagcaca catataagcg tgctctcaaa taagaaaggt agaatgctat gaatggaaag
     6961 cctcgataga ataacatatg cttgtccggt ttaaatgtga gaagttttta ataaaaacgc
     7021 tattttagta aggaacaagt ttaaaaggga acaacacttg acatgttaaa aattatgact
     7081 gaaaggtatt ttcatattac cattagtggg gttttaattc caataatggt tgttgagaaa
     7141 taaaaccttt attttgcaca acaaagcagc tagctgaagt agctaattgc tttattgtac
     7201 aattttagaa gttcactgga tacagatata tgttgtaaca aatagttaca tctatggtat
     7261 aaacaaatac caaaatatta tgcaggaaag aaaactaaat taaaacttca ttttataaag
     7321 agagaagcac aaaatattat atgtgtatcc agtcaattga gtgcgttttt actaacaaag
     7381 agcatctagc aaaagctaac tgttcggttc tccaaggggg aacaaggaga aagtaactta
     7441 atgggttgtc tacattattg acggaatctg gaggcttatt cggtcaaata aaaaaggtac
     7501 tgtatataag tgcactttgg accaagtttt ttatggtaac agttattggt attatgtgta
     7561 agaactaacg cgtatcttga aaataatgat tatttattta aatgtaaatt cataattaga
     7621 gtagaatcgc atcaatacgc tcgcagttaa ctacagtgat atctccgttt tcatctataa
     7681 atgtagcaag gcgggatcta ttatggaatc ctacaaatat agaagctttt aatattgtgc
     7741 cgttaactaa cagctgtttt attttttttc ctaagtaata tgtttctaaa agatagcaaa
     7801 tatttttaga actctctttt tgggttggga taaaagaacg tcggttattt ggaagatatg
     7861 ttagattagg attgaatact ttgggagtaa tagcatagtt gatgaaaact gacatttttc
     7921 cggctcctct attttataat ttatattgaa atatgctgtg tgaaataggg cttttttcaa
     7981 agccccatct ttactctact cagcttcaat tctaagcgct tgaatatcac gacagtccaa
     8041 aacaattata gagccaggtt ctgttgtgtt atccacaaat gttacacaaa aattatctgg
     8101 attaaagtta gtaaaaataa gatcctctag tgttgttcct gtaaggaaaa catctacttc
     8161 tgtacctaac tgtaatcttt ctaattgttc gcaaacacat tttgtatgct cagccatata
     8221 aaaacacctc cttttctcaa attactatta atatatgtat aaattaaata gaacgattgg
     8281 agtaatagct tagtaatatt aattattttg ctagttaata tttttataaa ataatccttt
     8341 gaaatgaata tcgtccggct agaaaactag aggacaccaa ttcattaaag cagccattaa
     8401 agctgtttta ggaataggtg tcctttttat tttgaaaagg gagatgggga aatgaatgtg
     8461 ttaagagatc aattaagtga atagaaaaag taatcgaaac aaataaggaa gaaaaaaata
     8521 tagagttcaa gtggttgtga gataaattga aaatagaaat tgataattaa aaagataaat
     8581 aattaaaaat ttggatgtag tgaatgcaaa tgaaattaaa attaaaaagg tataatttta
     8641 atttttaagt taagtataga aaagaaatga atcatctaga tataaagaaa ggatggattt
     8701 ttgcaatgag ctcaaatgaa tcaaatttca gttttaccag taacaaagat attgtaaagt
     8761 accttaatgt aagaaaagac cctgttgatt taagagacaa aatatttcgg agttacttgt
     8821 ttagtaatga ggcggatttg ccaacgcaag tcgatcttcg tcctaaaatg tcaggtatag
     8881 taaaccaatg gaatataggt tcttgtacgg cgaatgcaat tgtttcaggg ttaagagaat
     8941 acctattatt acaatcaggt cgtccattta cttgcttatc tcgtttgttt ttatattatc
     9001 atgagcgaga aatagaagga catattcagg aagatagcgg agcgaatgtt cgtgatggta
     9061 tggatgttct taaaaaaatt ggggtatgtc ctgaagagga ttttccttat actgagaatt
     9121 ttagagataa gccatcacca aaggctgaat cagatgcaac gctttataaa attggaacct
     9181 accatcgtat tcaagattta tcgggattaa aggctgcact tgcccatgga ttagttgttg
     9241 tacttggatt tgctgtgcca aaatctttta tgtcagaaga agttaaaaag actggtatag
     9301 ttccggtatc aggaacagat gatgtaattg ttgaagatgg tcaacaggca ggacatgctg
     9361 tttgtgctgc tggatatgat gataataaga aaactgttat tattcgaaac agctggggag
     9421 aagagtgggg agataaagga tattgttatt tcccatatga attatttgaa aatggaattg
     9481 tacaagatat gtggacaggt cgttaattag ataaagaagt ggaagtatgg aaacaataaa
     9541 acacgttata catgttacgg gtagtataaa agagcttgaa aaagatgtgg aaataaaggg
     9601 agtcaggttt gttacctaca acggaaaaag atatgagtaa tatttttata caagctggta
     9661 ttttaagtga acgaaaaaca aatatagtcc ggctagaaaa ctagaggaca ccaattcatt
     9721 aaagcagcaa ttcaagctgc tttaggaata ggtgtccttt tttttatttt ggaaagggag
     9781 atggggaaat gaaggtgtta aagaatcagc tacgtgaatg gaaaaaccaa tcgaaaaaga
     9841 aaaataagaa aaaacgaaaa gagaaattta gtactcgtga aattgaggat ttgatggggg
     9901 tgcatagacc ttgttatgaa cgcagacgtg gagcattaag acaaaagtaa tttaaaaata
     9961 aaaaggagtg gtcttacatg actaaacaat tatctttctt accaaaaatt gatagaacag
    10021 cgacacaaga ggaattagaa ggtgtgttgg aaagtgtacg tatacataga caatttggga
    10081 tgatgcgtaa agaaatgaaa gtcactcctt cttatgaaat acgtgagcac ggtcctacac
    10141 atacagttgg taagctgtta gaagatgttg ctatagcaaa tattcaacaa agcaaacgag
    10201 aagagtggct tgaaagaatg tcagtacgta ttgatcagtt tctaaatcga ttagggaacg
    10261 gacgtgcagg aagcattcaa agagatatta tttataaacg ttatttagaa gaagaagacg
    10321 tatg
//
