LOCUS       NZ_CP035999            10339 bp    DNA     circular CON 10-DEC-2024
DEFINITION  Bacillus mycoides strain BPN37/1 chromosome, complete genome.
ACCESSION   NZ_CP035999
VERSION     NZ_CP035999.1
KEYWORDS    RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus; Bacillus cereus group.
REFERENCE   1  (bases 1 to 5322329)
  AUTHORS   Fiedoruk,K., Drewnowska,J.M. and Swiecicka,I.
  TITLE     The dynamics of pangenome and proteome of selected Bacillus cereus
            sensu lato representatives from the primary and anthropologically
            changes environments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5322329)
  AUTHORS   Drewnowska,J.M., Fiedoruk,K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Department of Microbiology, University of
            Bialystok, 1J Ciolkowskiego, Bialystok 15-245, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 108.48400000000001x
            Sequencing Technology :: Illumina MiSeq and MiniIon Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018739505.1-RS_2024_12_10
            Annotation Date                   :: 12/10/2024 03:54:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,286
            CDSs (total)                      :: 6,132
            Genes (coding)                    :: 5,884
            CDSs (with protein)               :: 5,884
            Genes (RNA)                       :: 154
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 248
            CDSs (without protein)            :: 248
            Pseudo Genes (ambiguous residues) :: 0 of 248
            Pseudo Genes (frameshifted)       :: 114 of 248
            Pseudo Genes (incomplete)         :: 146 of 248
            Pseudo Genes (internal stop)      :: 66 of 248
            Pseudo Genes (multiple problems)  :: 68 of 248
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:59:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 647112
            Orig. end                         :: 657451
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035999.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10339
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10339
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10339
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5339](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5339)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    384..400
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.8"
                     /tool="antismash"
     gene            complement(484..1368)
                     /locus_tag="EXW27_RS03225"
                     /old_locus_tag="EXW27_03285"
     CDS             complement(484..1368)
                     /EC_number="2.3.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526760.1"
                     /locus_tag="EXW27_RS03225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_03285"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_078179104.1"
                     /transl_table=11
                     /translation="MGNVEKMANLLLEEPRREQLFPLFEEVFGITSQTLQDFSEKGYWD
                     YTYKALSFLQDDNAIANVAAFSLPLLINGEKINATGIQSVMTHPNYRRRGLMKQLFSKM
                     IEEIDEQCECAVLFTENPELYKPFGFQIVQEYLMTVSYENKGYKDSQLKKLDFYNEEDR
                     QLIHEIIENGQRLSNKFSTLNYRSSFYLNMYDSTWNENLYYSEKLDALIVFTVENQKLK
                     LFGVFAPVIPILDELCEEISGEFTEIEFYFYPDQLGIDDVQYTELQTSKYLMVRSNKEL
                     DFKGYKFPVLTEF"
     gene            complement(1631..2320)
                     /locus_tag="EXW27_RS03230"
                     /old_locus_tag="EXW27_03290"
     CDS             complement(1631..2320)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002031964.1"
                     /locus_tag="EXW27_RS03230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_03290"
                     /product="MOSC domain-containing protein"
                     /protein_id="WP_078179071.1"
                     /transl_table=11
                     /translation="MTKAYEVLSLNIGLPKEVMYGGKLIQTGINKKQVKEPVFLSFLKL
                     NGDGQADLVHHGGVDKAVCVYTGDHYPYWEKELNQELVYGAFGENITVSGMREEDVCIG
                     DTFQLGEAIVQVTQPRQPCFKLAKKYNIPKLPLYFQDTGYTGFYFRVLKEGWILPVDTL
                     KLLKPDPKGVSILFANRIMHKEKQNSEGLKRILEVNALSTSWRKTFEKRVKGEEISTKE
                     RLEGTKE"
     gene            complement(2335..3660)
                     /gene="gabT"
                     /locus_tag="EXW27_RS03235"
                     /old_locus_tag="EXW27_03295"
     CDS             complement(2335..3660)
                     /EC_number="2.6.1.19"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (34-374). E-value: 1.8e-122.
                     Score: 400.5. Matches aSDomain:
                     nrpspksdomains_EXW27_RS03235_Aminotran_3.1"
                     /codon_start=1
                     /gene="gabT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200502.1"
                     /locus_tag="EXW27_RS03235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_03295"
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_078179070.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.6e-132, bitscore:
                     439.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKSSVLPGSYSQVLQKRYEEAVPTGIYHLTPLYVKEARGAIITD
                     VDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAESFIHPCFHVTPYENYITLAEKINEK
                     VPGTSKKKTMFANSGAEAIENAVKIARKVTGRSKVISFERGFHGRTLMTMSLSTKETPY
                     KAGFGPFVSDMHILPYPYYYRAEEGVSPEEVDSQVIYQFEKFLEEVSPEKVAAIVMEPV
                     QGEGGFIVPSISFVKRLREICNQYGILMIMDEVQTGFGRTGKWFATEHFHIEPDIMTLS
                     KSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGSPLSCAAGLAVIEIMEEENLVERAQQ
                     IGNRIMSHLHQMQLKYEVIGDVRGIGAMVAMELVTNRITKEPAKELTVKLIEDFWKNGL
                     ISIGAGIFDNVLRFLPPLVISNEEIDRGFEIINESFEKLCRK"
     aSDomain        complement(2539..3558)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EXW27_RS03235_Aminotran_3.1"
                     /evalue="1.80E-122"
                     /label="EXW27_RS03235_Aminotran_3.1"
                     /locus_tag="EXW27_RS03235"
                     /protein_end="374"
                     /protein_start="34"
                     /score="400.5"
                     /tool="antismash"
                     /translation="VKEARGAIITDVDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAE
                     SFIHPCFHVTPYENYITLAEKINEKVPGTSKKKTMFANSGAEAIENAVKIARKVTGRSK
                     VISFERGFHGRTLMTMSLSTKETPYKAGFGPFVSDMHILPYPYYYRAEEGVSPEEVDSQ
                     VIYQFEKFLEEVSPEKVAAIVMEPVQGEGGFIVPSISFVKRLREICNQYGILMIMDEVQ
                     TGFGRTGKWFATEHFHIEPDIMTLSKSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGS
                     PLSCAAGLAVIEIMEEENLVERAQQIGNRIMSHLHQMQLKYEVIGDVRGIGAMVAMELV
                     "
     gene            3790..4692
                     /locus_tag="EXW27_RS03240"
                     /old_locus_tag="EXW27_03300"
     CDS             3790..4692
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003203974.1"
                     /locus_tag="EXW27_RS03240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_03300"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_085963395.1"
                     /transl_table=11
                     /translation="MISIELRQLEYFLAVSKELHFTKAAEKLNISQPSLSQQIRALEHE
                     VGMPLFDRIGKKISLTEAGRVLLSHSKTIFHEVEQARSAIQDLNGLHHGSLTIGSLLTV
                     VNYLLPPAILDFNKQYPNIELSVLGLRTGEIREKLLQNELDIGITFLPVQDKEIVSIPL
                     YKSELTLVVPTSHSLTERKYVSIEELQDYPLILLPKNFYLTELITSHCQNFNFKPKPIL
                     EISTMESLIQMVAQGMGITVLPKPYIDFLQNEHIQAIKIENPTPTIDIGLIYRRDKYMC
                     AATRKFIEQLKITVHSLQK"
     gene            complement(5001..5339)
                     /locus_tag="EXW27_RS03245"
                     /old_locus_tag="EXW27_03305"
     CDS             complement(5001..5339)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002189588.1"
                     /locus_tag="EXW27_RS03245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_03305"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_147740257.1"
                     /sec_met_domain="TIGR03601 (E-value: 2.8e-30, bitscore:
                     102.9, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNFQQELQALSLNDYQPGNIVYWDQQNQYPYYYITDDARRCGGC
                     GGGRCGGCGGCGRCGGGRCGGFRCFGCFGCFSCFGCGGCSNCSNCSNCSNCSNCFDGFN
                     GTTVTFEY"
     misc_feature    6034..6051
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.83"
                     /tool="antismash"
     gene            6282..6575
                     /locus_tag="EXW27_RS03250"
                     /old_locus_tag="EXW27_03310"
     CDS             6282..6575
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002168401.1"
                     /locus_tag="EXW27_RS03250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_03310"
                     /product="hypothetical protein"
                     /protein_id="WP_002168401.1"
                     /transl_table=11
                     /translation="MCNSKPIDELTIEDLKQNPIWEWAIDEAENEECDETWVKPVETIN
                     FTEELNGSIVLGELIIHNDEKFPMMCSIDIENNEVLISSIVFITKKKMSILL"
     gene            complement(6822..7865)
                     /locus_tag="EXW27_RS03255"
                     /old_locus_tag="EXW27_03315"
     CDS             complement(6822..7865)
                     /EC_number="1.2.1.11"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1219: N-acetyl-gamma-glutamyl-phosphate reductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200507.1"
                     /locus_tag="EXW27_RS03255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_03315"
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="WP_002168402.1"
                     /transl_table=11
                     /translation="MSEKSYHVAVVGATGAVGQKIIALLERVTKFNIEEITLLSSKRSA
                     GKIVQFKGREIKIQEAKSTSFEGVDIAFFSAGGEVSRQYVNHAVSSGAIVIDNTSEYRM
                     AHDVPLVVPEVNAHTLREHKRIIAVPNCSALQMVTALQPIRKSFGLERIIVSTYQAVSG
                     SGIHAIHELKEQAKSMLTGEEVKSTILPVKKDKKHYPIAFNVLPQVDIFTDNDFTFEEV
                     KMIQETKKILEDPNLKMAATCVRVPVVSGHSESVYIELEKEATVAEIKEVLLNAPGVIL
                     QDNPSEQIYPTPLYAEGKRDTFVGRIRKDPDTSKGFHLWIVSDNLLKGAAWNSVQIAET
                     LVENGII"
     gene            7995..8867
                     /locus_tag="EXW27_RS03260"
                     /old_locus_tag="EXW27_03320"
     CDS             7995..8867
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016095293.1"
                     /locus_tag="EXW27_RS03260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_03320"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_002127351.1"
                     /transl_table=11
                     /translation="MEMRHVKTFCAIVKYGSFSKAAHALGYAQSTVTAHIKALENDLNI
                     PLFDRLGKKILLTKAGHQFHPYALELLTIYDKAQEIPQNSDHLEGTLAITSNESLAVYR
                     LPQLLRTYKQENPKVNIVLETNTNEQALKKLHEGETDVIFIIGESIEHNDFITCNLCNE
                     TFGWIFPAHFPSNTDPLLLLQDTQFIFTEQSCGYRPMVDRFLRQSGRNPNKTFETSNVE
                     VIKQSVMCELGISILPYIVVQESNQKEQLCFQPIETPAVIQSHVIYHKSRWISPVLQSF
                     LTLLERIEK"
     gene            complement(8973..9578)
                     /locus_tag="EXW27_RS03265"
                     /old_locus_tag="EXW27_03325"
     CDS             complement(8973..9578)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853587.1"
                     /locus_tag="EXW27_RS03265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_03325"
                     /product="PRK06770 family protein"
                     /protein_id="WP_078179068.1"
                     /transl_table=11
                     /translation="MKTLFKIIGIIAGMAVIGVGLTYGMLYYLNNSKPAAKKASPAAPA
                     VEVLADSNVKAEDAKRLENGNYSLPNSGFNKNFKWTDENIQTALHEMAHQKAKADQKWG
                     YIFITQERIESLLEIINSNDVAQKSTYVDILERWKQGKYEKVDVDHNKIWKLQSGNLGE
                     GKGVMSEKEQKELINQVFIQKGTYSGSKLIAGEVQANK"
     misc_feature    complement(9685..9702)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.83"
                     /tool="antismash"
ORIGIN
        1 cgttttcaca tatattgtat aatcgccaag ttttcgaata attcaaaaac gtaattgttg
       61 cttagcaaac tcatttggat agtcttgcgt tgaccaatca gaatagccat tattcgttac
      121 gacagcacct aaatgtgatg ggccctcagg aatatattct aaagatgttt ttaaccaaca
      181 cttctcagaa atcataacaa ataaacccgc ttgatcgtat gtcgcatttg gattcatgcg
      241 caattgaacc tcagcctgaa aatctgtcat tatctcatca taaaatacat ggccattcat
      301 tacttcaaaa ccataatggg ttttcagcca aaaatctgtt tcttctttcg aatggacaat
      361 gaatgtatca ttgtcttcaa gtttgtaaaa tttaggtgcg ttcattacag taagttgctc
      421 aatatgattt ggtatatatg atgttttcat aattcctcca taaaaaagca tttaaattaa
      481 tatttaaaac tcagttaata ccgggaattt ataaccttta aaatctaatt ctttattact
      541 tcgaaccatt aaatacttac tagtctgtaa ttctgtatat tgtacatcat caattcctaa
      601 ttgatctggg tagaaataaa attcaatctc cgtaaattcc ccagagattt cctcacataa
      661 ttcatctaaa atcggaatta ccggcgcaaa tactccaaat aattttagtt tttgattttc
      721 tactgtaaaa acaatcagcg catctaattt ctctgaataa taaaggtttt cattccatgt
      781 tgaatcatac atatttaaat aaaacgaaga gcggtaattt aacgttgaaa atttattaga
      841 aagcctttgg ccattttcaa taatctcatg tattaattgt ctatcctctt cattataaaa
      901 atctaacttt ttaagttgag aatctttgta acctttattt tcgtatgata ctgtcatcaa
      961 atattcttgc acaatttgaa aaccaaatgg tttatatagt tccggatttt cagtaaacaa
     1021 gaccgcacac tcgcattgct catcaatttc ctctatcatc ttactaaaca attgtttcat
     1081 taatcctctt ctacgataat tcggatgtgt cattactgat tgaatacccg ttgcatttat
     1141 tttttcacca ttaattaaaa gcggaagtga aaatgccgca acatttgcaa tcgcattatc
     1201 atcttgtaaa aatgataatg ctttatatgt ataatcccaa taccctttct ctgaaaaatc
     1261 ctgcaatgtt tggctcgtaa tcccaaacac ttcttcaaat aaaggaaata actgttctct
     1321 tctcggttct tctaatagca agtttgccat tttttcaaca ttccccattt tgtccctcca
     1381 tacgtcgatt ccattaattt agatatactt atatgaccta ctctcaaaaa ataggccttt
     1441 caaatcaata tagtatcata aaacttttaa aaatggttta atttatttta tgttttgatt
     1501 attaaaaaac aagtcgaata aactagtatt aagatttttg aataatatca tgtttattat
     1561 ataacaaatg gtgcttccta cttatctttc cgataataga aatacaccat tttatgcaaa
     1621 tataattcac ctattctttg gttccctcaa gtcgctcctt cgtactaatt tcttctccct
     1681 tcactcgctt ttcaaacgtc tttctccaac tagtagacag tgcatttact tctaaaatcc
     1741 tttttaatcc ttcagagttt tgtttttctt tatgcattat gcgattcgca aacaaaattg
     1801 acacaccttt tggatcaggc ttcaacaatt ttaaagtatc taccggtaat atccaccctt
     1861 cttttaatac tcgaaaataa aaacctgtgt atcctgtatc ttgaaaatat aatggcaact
     1921 ttggaatatt atatttcttc gctaatttaa aacaaggttg tcttggctgc gttacttgta
     1981 caatcgcttc tccgagttga aacgtgtcac caatacaaac atcttcttca cgcattccgc
     2041 ttactgttat attttcaccg aaagccccat atacaagttc ctgattcaat tctttttccc
     2101 agtacggata gtgatcacca gtataaacac aaaccgcttt atctacccct ccatgatgaa
     2161 ctaaatccgc ctgtccatct ccattaagct ttaaaaatga taaaaaaaca ggttccttta
     2221 cttgcttttt atttatccct gtttgaatta acttcccacc atacataacc tcttttggca
     2281 gtcctatatt tagagatagg acttcatatg ctttagtcat tcaaatccct cctcttactt
     2341 gcggcacaac ttttcaaaag actcatttat tatttcaaat cctctatcta tttcttcgtt
     2401 tgagataact agaggtggta aaaagcgtag tacattatca aatatccccg ctccaatact
     2461 tataagccca ttcttccaaa aatcttcaat tagctttact gttagttctt ttgcaggttc
     2521 tttcgttatg cgattcgtta caagttccat tgcaaccatc gcaccgattc cacgaacatc
     2581 accaatcact tcgtacttta attgcatttg atgtagatgt gacattattc tatttccaat
     2641 ttgctgagcc ctttcaacta gattttcttc ttccattatt tcaattacag caagaccggc
     2701 tgcacatgat aacggactcc cggcaaaagt tccaccaatt tgtcctctcc caggaatgtc
     2761 catcaactcc gcacgaccga caatagcact taatggtaaa ccgccagcaa ttgattttga
     2821 caaagtcatt atgtcaggtt ctatatgaaa atgttccgtt gcaaaccatt tccctgttcg
     2881 gccaaatcca gtttgcactt catccattat cattaaaata ccgtattgat tacaaatttc
     2941 tcgtaatcgt tttacaaatg aaatggaagg aacaataaac ccaccctctc cttgtactgg
     3001 ttccattacg atcgctgcta ctttttcagg tgatacttct tctaaaaact tttcaaactg
     3061 atagatgact tgagaatcca cttcttcagg agaaactcct tcttcagctc tgtaatagta
     3121 cggatacggc aatatatgca tgtctgaaac gaacggacca aatcctgctt tatatggagt
     3181 ttctttggtc gataaagaca ttgtcattaa tgttctccca tgaaatcctc gttcaaacga
     3241 aattacttta ctcctccctg ttactttacg tgcgatcttt acagcatttt caatcgcttc
     3301 cgcaccacta ttggcaaaca tcgttttttt cttacttgta cctggcactt tctcattaat
     3361 tttctccgct aaagtaatat aattttcata tggagttaca tgaaaacaag gatgtataaa
     3421 cgattctgct tgtttttgta ccgctcttac aactttcgga tgacaatgtc caacattttg
     3481 cataccaatt ccaccagcaa aatcaataaa ctgatttcca tctacatctg ttattatcgc
     3541 tccccttgct tcttttacat ataaaggcgt taaatgatat attcctgtcg gtacggcctc
     3601 ctcatacctt ttttgaagta cttgtgaata actgcccggt aatacactag atttcaacaa
     3661 ttcttcttca cctcatctaa ttttttaatc aatccatttc tgataaagtg attatatatc
     3721 ccacaaaacc attgttcaat tcaattattt atataataat gataggtata tcctatgatt
     3781 aaacttgagg tgatttcaat cgaattacga caattagaat actttttagc tgtttcgaaa
     3841 gaattacatt tcacaaaagc agccgagaaa ttaaatattt cacaaccttc actaagccaa
     3901 caaatacgtg cattagaaca tgaagtaggc atgccacttt tcgatcgaat tggtaaaaaa
     3961 atatctttaa ctgaagcagg aagagtttta ttatcgcaca gtaaaacaat ctttcacgaa
     4021 gtcgaacaag ctcgttctgc cattcaagac ttaaatggat tacatcatgg ttccttaaca
     4081 ataggatctt tgttaaccgt agtaaattat ttactaccac cagctatatt agatttcaat
     4141 aagcaatatc caaatatcga actctctgtg ttggggcttc gtacaggaga aattcgtgaa
     4201 aaattattac aaaatgaatt agatattggt attacatttc tcccggtgca agataaagag
     4261 attgtttcta tcccactata taaaagtgaa cttacattag ttgtgccaac cagccattct
     4321 ttaactgaac gtaagtatgt atctattgaa gaattacagg actatccttt aattcttcta
     4381 cctaaaaatt tctatttaac agaactaatt acatctcatt gccaaaactt taactttaaa
     4441 ccaaagccaa ttttagaaat tagtacaatg gaatctttaa ttcaaatggt cgcacaaggt
     4501 atggggatta ccgtgttacc aaaaccgtat atagactttt tgcaaaatga gcacatacaa
     4561 gctattaaaa ttgaaaaccc cactccaaca atagatatag gacttattta tagaagagat
     4621 aaatatatgt gcgctgctac ccggaaattt attgagcaac tcaaaataac agtacattct
     4681 ttacaaaaat aagcataaaa agagattatc ctatgcagcc gttacacaac gacctattag
     4741 aataatctct ttttataatg atataaaaca ttacatttta tggtttcttt tctaataacc
     4801 tattattcat tataagtgct aaattattat cgtattatta agctacacat cagcgatatt
     4861 ttatgtctat ttcgtatagg tgttagcaag tccgtttgca aacttgttag cacattttta
     4921 taaaaattat ataatgcttc acaaagtagg aatccgtttc gatcaatctt ttttcaaaat
     4981 ggattccttt ttgtctctta tcaatattca aatgttacag tagtaccatt aaaaccatca
     5041 aaacaattag agcaattaga gcaattagag caattagagc aattagagca accaccacaa
     5101 ccaaaacaac taaagcaacc gaagcaacca aaacaacgga atccaccaca acgaccaccg
     5161 ccacaacgac cacaaccacc acaaccacca caacgaccac cgccacagcc gccacagcga
     5221 cgagcatcat ctgtaatata gtaatatgga tattggtttt gctggtccca atacacaata
     5281 tttccaggct gataatcatt aaggcttaac gcttgtagtt cttgttgaaa attattcatc
     5341 ttcatgacct ccgtttataa actaaaaccc catcgatact ttcctatatt tctgttcgtt
     5401 aaatctaagt agaaatagcg ctataaagtg aaactttaat cagtaggggt tttgttcatc
     5461 cccactgatt attagcccgc accaatcggg cgtttacggg cagcagggct cccacctaac
     5521 ttctttgctc cagttgaatt ttgaggtggg agttttactg cccacaaata gcgggataaa
     5581 ctaagttcaa tacgtacacc aacaaaatat gacctatcac tagatgatgc accttgttca
     5641 tatgcctatt tttaggttat ttttactatg ggctcatttt tataaagtga aactttaatc
     5701 agagggggtt tacgggcagt taatgcgggc taaatggaca ttataaaata tgaatttaaa
     5761 caataatatt tagatttatg tcatcatttt ctagttctat agtcgtatat tccataattt
     5821 tgtttggtca tcttcgtatt tccaatatgc actcccatct ttatttttct tcgcttcaat
     5881 atctgctata tcagagctaa ttttataagt atttataatt agtgatttct gctaagaaaa
     5941 gtctattttt atgaatggca aatcagcgat gttttttgct atttccccca tcacaaccac
     6001 ggttttcctt ttcaaaaggc attatttact gtctagttaa aaccttgtta agattaccgt
     6061 aagaatagac ttttaatagg ttgtaaaaat aagttcaaca ttacaaaact agatttaata
     6121 atatcaaatg gataatactc tacatcgaaa cttcttattt gcgatcaact atttattgaa
     6181 acgagcaaac ttgtatttat aggattgcaa atagccacat tttacaatta atgaataaat
     6241 tttttactca tccattttca tggcaaaagg agtatatatt aatgtgtaat agcaaaccaa
     6301 ttgatgaatt aactattgag gacctgaaac aaaacccaat atgggaatgg gcaatagatg
     6361 aagcggaaaa tgaagagtgc gatgaaacgt gggtaaaacc agtggaaaca ataaatttta
     6421 cagaagaatt aaacggttca atagtacttg gcgaattaat aatacataat gatgagaaat
     6481 tccctatgat gtgtagtatt gatattgaaa ataatgaggt attaattagt tccattgttt
     6541 ttataacgaa aaagaagatg agtatattgc tatagaagat gtagtgaaaa aggttgaaat
     6601 gcctttatca cttaatatta atctcacaat taacggagag cctagatttt taaaattctc
     6661 tgcagataaa gttgatattt ataaaaacac tataaaaact aacttaaatt aaattttaat
     6721 tgaagtaaga ggaaaatact cattaagtaa aaaacaggaa accattataa ttaacggttt
     6781 cccaaatatc cttctatttt caatatttat ataaatccat actaaataat cccattttcc
     6841 accaatgtct ctgcgatttg aactgagttc caagcagcac cttttaataa attatcagaa
     6901 acgatccaaa gatggaatcc ttttgatgta tcaggatctt tacgtattct acctacaaat
     6961 gtatctcttt taccctctgc atataatggc gttggataaa tttgttcact aggattatct
     7021 tgtaaaataa cacctggtgc attcagtagt acttctttaa tttctgcaac ggtcgcttct
     7081 ttttcaagtt caatataaac tgattcagaa tgccccgata caactggaac tctgacgcaa
     7141 gtagctgcca ttttcagatt tggatcttct aaaattttct tcgtctcttg aatcatcttt
     7201 acttcttcga atgtgaaatc attgtctgta aatatatcca cttgcggcaa tacattaaat
     7261 gcaattggat aatgtttttt atcttttttc actggtaata tagtgctctt cacttcttca
     7321 cctgtaagca ttgacttagc ttgttctttt aattcatgaa tcgcatgaat tcctgagcct
     7381 gatacggctt gatacgttga aacaataatt cgttctaaac caaatgattt tcgaattggc
     7441 tgaagagctg ttaccatctg tagtgctgag caattcggaa ctgcaatgat ccttttatgt
     7501 tcccttaaag tatgtgcatt tacttctgga acgacgagtg gtacatcgtg tgccattcgg
     7561 tattcacttg tgttgtcaat tacgattgca ccactagaaa ctgcatgatt tacatattgt
     7621 ctagaaactt ctcccccagc gctaaaaaag gcaatgtcta caccttcaaa actagttgat
     7681 tttgcctctt gtattttaat ctctcgtcct ttaaattgta ctatcttacc agcagatcgt
     7741 tttgacgaga gtaatgtaat ttcttctata ttaaatttcg ttactctctc taacaatgca
     7801 atgatttttt gccctactgc gcctgtagct ccaactacag ctacatgata gctcttttca
     7861 ctcatttttt ctcctccaat ttcaattgaa tgaatttctc cacaacttaa ttttaaaaga
     7921 aatttaatga tttgaaaaat tgaaatacat gatataatca ttcacaaatc atgataataa
     7981 aagaggtatc atttatggaa atgagacatg ttaaaacatt ttgtgccatt gttaaatatg
     8041 gtagcttttc taaagctgct catgcattag gttatgcgca atctactgtg acagcccata
     8101 ttaaagcatt agagaacgat ttaaacattc ccctattcga tcgattagga aaaaaaatac
     8161 ttcttacaaa agcaggtcat caatttcatc cgtatgcatt agaattactt actatttatg
     8221 ataaagcgca agaaatccct caaaacagtg accatttaga aggaacattg gctattacat
     8281 ctaatgaatc attggcagtg tatcggttac cacaattatt acgtacttac aaacaggaaa
     8341 atccgaaagt aaatatcgta cttgagacaa ataccaatga gcaagcattg aaaaaattac
     8401 atgaaggaga aaccgatgtt atcttcataa ttggagaaag tatagagcat aatgatttta
     8461 ttacgtgcaa tttatgtaat gaaacatttg gatggatttt ccctgctcat ttcccttcaa
     8521 acactgatcc actcctttta ttacaagata ctcaatttat atttacagaa caaagctgtg
     8581 gctatagacc aatggtagat cgttttttac gtcaaagcgg aagaaatcct aataaaacat
     8641 ttgagacttc caatgttgaa gtcattaaac aatccgttat gtgtgagtta ggaatttcta
     8701 ttctccctta catcgttgta caagaaagta atcaaaaaga acaactctgt tttcagccca
     8761 tcgaaactcc agccgtcatt caaagccacg taatctatca taaatctaga tggatatccc
     8821 cagttttgca atcgtttctt acattattag agagaataga aaagtaagcg agtaattaca
     8881 aaaatgagta actgctctaa aaagcaatta ctcatttttg ttaaaacatt tgtgttcata
     8941 acaatacagg tgtttcaaaa tcaatatgta tattatttat tagcttgtac ctcacctgca
     9001 attaatttac tacctgaata tgtacctttt tgtataaaaa cttgattaat taattctttt
     9061 tgctcctttt ctgacattac tcctttccct tctcctaaat tcccactttg taatttccaa
     9121 atcttgttgt ggtccacatc aaccttttca tattttcctt gtttccatcg ttctaaaata
     9181 tccacatatg tacttttttg tgcaacatca ttactattta taatttcaag taaactttca
     9241 atacgttctt gtgtaataaa aatatagccc catttttgat cagctttcgc tttttgatgt
     9301 gccatttcat gtaatgctgt ctgtatgttc tcatctgtcc atttgaagtt tttattaaaa
     9361 ccactattcg gcaatgaata atttccattt tctaatctct ttgcatcttc ggcttttaca
     9421 ttactatcag ctagcacttc tactgccgga gcggctggtg atgccttttt cgcagctggt
     9481 ttactattat tcagataata cagcatacca tatgttaaac ctactccaat tactgccata
     9541 ccagctataa ttcctattat tttaaataat gttttcatct tgcctatttc ctcccatatt
     9601 tcccttttca taatatctat ttatcaaaaa cagatgtagc ttctcttttt tcgcaacttg
     9661 ctgcgattca tttttccata ctaattaaca taaatataac tattactttc cagaaatgta
     9721 gcaactccat cctcatcatt acttttggca ataaatttag cgacggattt taattcttca
     9781 acagcattgc tcatcgctac gccaatccca cacttctcta tcatttccat atcattgtag
     9841 tcatctccaa atgcaattac ttcagataca tctcgattta agtgctgtag aacatgctga
     9901 atactattcc attttgaaac atccgtatgc ataatttgac acaatgttcc gttatctgta
     9961 atgactgcct tgcactcatc aggcaactcc ttagtgaact ccttatttat tgagccatta
    10021 gtacaaacaa ttactttaga ggctttttca aagtttaatt ctcttatatc catgacttca
    10081 cacggcacat taccaaaata atcagtaaca tcaaaatttg agtacagctt atcatttatt
    10141 tcaagacaaa tctgatgaag attatacttt tttgctattt ctatgattcc taaaatatca
    10201 tttttagaaa tcttcatttc atataaaata tcattacctt taagaactag agctccatta
    10261 taacaaatga taatctcatt ctttaacacg ttgggaatat actgatttat agctcttgga
    10321 ggccttgccg tagcaaaaa
//
