LOCUS       NZ_CP035999            10276 bp    DNA     circular CON 10-DEC-2024
DEFINITION  Bacillus mycoides strain BPN37/1 chromosome, complete genome.
ACCESSION   NZ_CP035999
VERSION     NZ_CP035999.1
KEYWORDS    RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus; Bacillus cereus group.
REFERENCE   1  (bases 1 to 5322329)
  AUTHORS   Fiedoruk,K., Drewnowska,J.M. and Swiecicka,I.
  TITLE     The dynamics of pangenome and proteome of selected Bacillus cereus
            sensu lato representatives from the primary and anthropologically
            changes environments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5322329)
  AUTHORS   Drewnowska,J.M., Fiedoruk,K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Department of Microbiology, University of
            Bialystok, 1J Ciolkowskiego, Bialystok 15-245, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 108.48400000000001x
            Sequencing Technology :: Illumina MiSeq and MiniIon Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018739505.1-RS_2024_12_10
            Annotation Date                   :: 12/10/2024 03:54:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,286
            CDSs (total)                      :: 6,132
            Genes (coding)                    :: 5,884
            CDSs (with protein)               :: 5,884
            Genes (RNA)                       :: 154
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 248
            CDSs (without protein)            :: 248
            Pseudo Genes (ambiguous residues) :: 0 of 248
            Pseudo Genes (frameshifted)       :: 114 of 248
            Pseudo Genes (incomplete)         :: 146 of 248
            Pseudo Genes (internal stop)      :: 66 of 248
            Pseudo Genes (multiple problems)  :: 68 of 248
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:59:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 349474
            Orig. end                         :: 359750
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035999.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10276
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10276
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10276
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5276](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5276
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            661..1572
                     /locus_tag="EXW27_RS01930"
                     /old_locus_tag="EXW27_01960"
     CDS             661..1572
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136580.1"
                     /locus_tag="EXW27_RS01930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_01960"
                     /product="DMT family transporter"
                     /protein_id="WP_002185732.1"
                     /transl_table=11
                     /translation="MHNKKWDFRIVCAHAFTILIWGTAFPGIRMGLEAYTPEHLTLLRL
                     LIASLILFLFSLIYKLRLPDLKDIPVIFIFGALGFTIYHIALNYGEKSVNAGPASLIVS
                     VTPILTAILASVFLNERMKLNGWIGGVISFVGIAFLSLSQGDSIQLNSGGLLILLAAIS
                     ESLFFVFQRPYLKKYGFLPFTIYTIWSGTVFMLIFLPGIYQEIVTAPIEITLSVIYLGV
                     FPTVLPYIALAYITSHASASEATSSLYLTPVTACIIAWIWLGEVPTFVSIIGGGITILG
                     VVIAHLTFNKDDYKQIESEKVW"
     misc_feature    complement(1590..1606)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.88"
                     /tool="antismash"
     gene            1856..2779
                     /locus_tag="EXW27_RS01935"
                     /old_locus_tag="EXW27_01965"
     CDS             1856..2779
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="EXW27_RS01935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_01965"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_002127616.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATGPLTNEITTYYPEIHGPEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGEDLTIVDVGRSTSLAIAFNLWNELMLNIKGIYFMGGVFLEAGNVTPLA
                     EANVYGDPIASQIVFQQAKNITLFPLNVTNKTVLTPSVFTYILVNTQNPFKSIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVLAISGLVNPTFLNYTSRKVTVDICGETKGQTFADFRPH
                     SKSEGARIALQLDEEKFIQDFMKIML"
     gene            2856..3320
                     /locus_tag="EXW27_RS01940"
                     /old_locus_tag="EXW27_01970"
     CDS             2856..3320
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136577.1"
                     /locus_tag="EXW27_RS01940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_01970"
                     /product="N-acetyltransferase"
                     /protein_id="WP_002168287.1"
                     /transl_table=11
                     /translation="MNVQLKIVTRENWEDALKLRVKENQIHLVPAVAVSLAKVYIKPDG
                     ENVEYIPFAIYDGDLIVGFIMHAFVRESTDMYWINGFIIDEKEQGKGYGKAALQEIINL
                     IKNNFKECEEIRLTVHKDNISAKKLYECYDFKPLGNVYDGEEVYRLLLLK"
     gene            3504..4073
                     /locus_tag="EXW27_RS01945"
                     /old_locus_tag="EXW27_01975"
     CDS             3504..4073
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002065694.1"
                     /locus_tag="EXW27_RS01945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_01975"
                     /product="DUF4256 domain-containing protein"
                     /protein_id="WP_078179191.1"
                     /transl_table=11
                     /translation="MKESNKKELSLEQREELLQTLKERFENNMNRHKGLEWANVQERLD
                     ANTEKLWSLNEMERTGGEPDVVDFGKEKGEYIFYDCSAESPKGRRSVCYDLEALESRKK
                     HKPENNAIDMATAMGIELLTEEEYRTLHNLENFDMKTSSWVQTPSDIRELGGALFCDYR
                     FGHVFVYHNGAESYYAARGFRGSLRV"
     gene            4446..4801
                     /locus_tag="EXW27_RS01950"
                     /old_locus_tag="EXW27_01980"
                     /pseudo=""
     CDS             4446..4801
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="EXW27_RS01950"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_01980"
                     /product="NUDIX domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKSKFHHIVRAIMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELREELGVTYSIKQFLGVIENQWQAKETLHHEINHIFEIDSNQ"
     misc_feature    complement(4972..4987)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 26.71"
                     /tool="antismash"
     gene            5001..5276
                     /locus_tag="EXW27_RS01955"
                     /old_locus_tag="EXW27_01985"
     CDS             5001..5276
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001869.1"
                     /locus_tag="EXW27_RS01955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_01985"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001001869.1"
                     /sec_met_domain="TIGR03601 (E-value: 7.5e-39, bitscore:
                     130.3, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNEFQQELQALNLNAYQPGNVVYWDQQQNQYPYYYIQDDARRCGG
                     CGGGGRCGGCGGRCGGCGGGRCGGCVGCVGCVGCFSCSNCWNWWFI"
     gene            5390..6418
                     /locus_tag="EXW27_RS01960"
                     /old_locus_tag="EXW27_01990"
     CDS             5390..6418
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002085304.1"
                     /locus_tag="EXW27_RS01960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_01990"
                     /product="serine hydrolase"
                     /protein_id="WP_002127614.1"
                     /transl_table=11
                     /translation="MYKYEKLISWVESIKETNQSSAAALCIIKDNKIVLEHYSGTHSNT
                     PTSKKVTISSQFNVASARKSYLGLMVAYAVYEGKIKSLDDEAVNYFKEYDPSLLGKTTI
                     RHLVTHSHGLNETDDGTAFREFEPGQAWAYRDINVRMVTQLIYQLYNKSFPELLKERVF
                     TPADFKKTGWRIKQDDNLVKVVVNPDEDAISSVGAVDDGSEKNLFVSAREFAFWGNLHL
                     NKGFLNGKQIVPKEVIALATSLQSPEYTNKELPQNGLFWFVQNEPALFSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSSSKNTMV
                     RV"
     gene            6450..6593
                     /locus_tag="EXW27_RS01965"
     CDS             6450..6593
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="EXW27_RS01965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002013225.1"
                     /transl_table=11
                     /translation="MKNEVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFEEI
                     EY"
     gene            6872..8080
                     /locus_tag="EXW27_RS01970"
                     /old_locus_tag="EXW27_01995"
     CDS             6872..8080
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="EXW27_RS01970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_01995"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_078179189.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAGKLQQKFGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYVNGNLINSLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNTATETLHNDYTPREMMKTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAIFNTIGRIILGTLSDKIGRLKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFQPTFITIGVLCVISFVISIVIRPPNAEKKKEIKSLNR
                     KIA"
     gene            8261..8677
                     /locus_tag="EXW27_RS01975"
                     /old_locus_tag="EXW27_02000"
     CDS             8261..8677
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016104977.1"
                     /locus_tag="EXW27_RS01975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_02000"
                     /product="N-acetyltransferase"
                     /protein_id="WP_002085307.1"
                     /transl_table=11
                     /translation="MQIVKQWVQEDSDYIREKVIEYNQKQLSNEEKTPSEKISFIVKNE
                     KEEIVGGITAITFWHHVYVDFLWVSEEYRHEGYGSNLMNLIEEFAIKKECRLIKLDTFS
                     FQAPNFYKKHGYKVIGVSEDYPKGHNHYYLEKRL"
     gene            8862..9305
                     /locus_tag="EXW27_RS01980"
                     /old_locus_tag="EXW27_02005"
     CDS             8862..9305
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="EXW27_RS01980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW27_02005"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_002085308.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDETHEKLLMVKNKGENGSYYTLPGGAVKFGETL
                     EEAAIREVKEETGLDISVKGVCSISEAFFEERGHHAIFFNFLGEITGGEICISRPKEIE
                     EITWMELDSAEPYLRIPEHLKSLLQKKETVPYIFNGTIIHQSS"
     misc_feature    complement(10244..10258)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.81"
                     /tool="antismash"
ORIGIN
        1 atgattttac ataattactt attgcttcac gtaaagggag atatcctttt tgttcttcat
       61 atccgaaata atctagttcc tcacatacgt tacgcataat gaattgcata gtttctaaca
      121 ggaaaagatg aggtgcaagt tctcctttac taagatgaat caatttttta tttgcttctg
      181 cttcatttat ttctcgtacc attaacttgc ttggtttatg aataccagcc tttacatatt
      241 cactccaatc gggcggggga ttggtggcta ataatgacca tgtattattc gttacaattg
      301 ttcctgatcc gattttccct tgaattaagc catctgccat aagttcttct agtgcaatta
      361 ttacagtact acgatttaca tgaaataatt tcgctaactg ccgctggcta ggaaccttac
      421 ttccaatcgg ccattctccg tattcaattt tttctttcat atagtctatt atctgccgat
      481 atttaggcag ttttttatca gtagacatat taaaaactcc ctcattcatt ttaaaaatgc
      541 aaattggttg gttttttcga ggattaactg gttgaagaca ttgtatcaac tattaaatat
      601 gatgaaaaga aataattggt aattagaaaa tggctggttt attggagagg gagagaatat
      661 atgcataata aaaaatggga ttttcgtatt gtatgtgctc acgcttttac gattcttata
      721 tggggaacag cttttccagg aattcggatg gggcttgaag cttacacccc tgaacacctt
      781 actttgctac gattattaat cgcttctttg attctttttc tgttttcact tatatacaag
      841 ttacgtttac ctgatttgaa agatattcca gtaattttta tatttggtgc tcttggattt
      901 acaatttatc atattgcact gaactacggt gaaaagagtg tgaatgctgg acctgctagt
      961 ttaattgtat cagtaacacc tatattaacg gcaattcttg cttctgtttt tttgaatgaa
     1021 agaatgaaat taaatggttg gatcggtggt gtaattagtt ttgtaggaat tgctttttta
     1081 tcgttaagtc aaggagattc cattcaattg aatagtgggg gattactaat attattggcg
     1141 gcaatttcag aaagtctatt tttcgttttt caaaggccat acttaaaaaa gtacggtttc
     1201 ttgccattta caatatatac aatttggtct ggtactgtat ttatgcttat ttttttgcca
     1261 gggatttatc aagaaatcgt aacagctcct atagaaatta ctttgagtgt tatatattta
     1321 ggtgtgttcc cgacagtact accgtatatt gcattagcat atattacatc ccatgccagt
     1381 gcttctgagg caacaagttc tctatattta acaccagtaa ctgcatgtat tatcgcttgg
     1441 atatggttag gtgaggtgcc aacctttgtt tcgataattg gagggggaat tactatactt
     1501 ggagttgtga ttgctcattt aacatttaat aaagatgatt ataagcaaat tgagagtgaa
     1561 aaggtatggt aagacatccc tacgagtatt tgcaatttga atgcaaaatt tcggattaat
     1621 aacaggtttt caattctata aatatagtga aaaaaatcta tattaaaatc agttagtcca
     1681 atatttgtac gagatttaat taaactggta gattatttat ctgcaaaaga actagcataa
     1741 tgctagttct tttttatcaa tacattactc accattaata tttgcttaga actgtataga
     1801 tttaattttt ttccacatat acataattat cttacctatg aaaggagtgc atttcttgga
     1861 gaaggtttta ttttttggag acccaggtat tgatgattcg tttgcaatta tgtatggctt
     1921 actgcatcct gaaattgaaa ttgttggtat cgtaactgga tatggaaatg ttgaacatat
     1981 acatgccgct cataatgccg catatatttt acagttagca aacagacaag atattcctgt
     2041 aattagcggt gcaacaggcc cacttacaaa tgaaattaca acgtattacc ctgaaatcca
     2101 tggaccagaa ggattagggc ccatccatgt accggaaagt gtgttatcta ttcctatata
     2161 caattttggt agtattgctg cgattcttgt aaagtatgga gaagatttaa caattgtcga
     2221 tgtaggaaga tctacctcgt tagcgattgc ttttaattta tggaacgaat tgatgttaaa
     2281 tataaaagga atttatttta tgggtggtgt ctttttagaa gcagggaatg ttactccatt
     2341 agcagaggca aacgtgtacg gagatcctat agcaagtcaa attgtatttc agcaagcgaa
     2401 aaatataacg ttattccctt taaatgtaac aaataaaacg gttttaaccc caagtgtatt
     2461 tacttacatt ttagttaata cacaaaatcc atttaagtcg attatgaaac ccatgtacga
     2521 ttattattta tcagcatacc aaaaactcaa tccatctatt gaagggccat tattacacga
     2581 tgtacttgct ataagcggtt tagtgaatcc tacattttta aattatacat cccgaaaagt
     2641 gactgtggat atatgtggcg aaacgaaggg acaaactttt gctgattttc gcccacactc
     2701 taaatctgaa ggggcgcgta tagcgttaca attagatgag gaaaaattta ttcaggattt
     2761 tatgaaaatc atgttataag ttgatgtcga aaaagggaat catttcactc cgttgaatat
     2821 aaaagataaa ttctctttta aaggagctta aaatagtgaa cgttcaatta aaaattgtta
     2881 cgagagaaaa ttgggaagac gcattaaagt tacgagttaa agaaaatcaa atccatcttg
     2941 ttcccgcagt ggcagtatca cttgctaaag tatacataaa accagatggt gaaaatgtcg
     3001 aatatattcc attcgctata tatgatggtg accttatagt tggttttatt atgcatgctt
     3061 ttgtacgaga gtcaacagat atgtattgga ttaacggatt tatcattgat gaaaaagagc
     3121 aaggaaaagg atatggaaaa gcagcattac aggaaatcat taacttaata aaaaataact
     3181 ttaaagagtg tgaggaaatt agattaactg tccataaaga taatatttct gcaaagaaac
     3241 tatatgaatg ttatgatttt aaaccattag gaaatgtata tgacggtgag gaagtatatc
     3301 gcttattgtt gttgaaataa agatgtactg ttttgaaaca ataagttgtt ccgttaaagg
     3361 gtcatattgc ggagaattgg ttgtactaat tcatataaat tatcgcaaac tcaattcacg
     3421 caattattta aacattttat tataaaacag tatttatata tacttaaact atcattaaca
     3481 aaaacatcaa tggaggaact aatatgaaag aaagcaataa aaaagagttg tcactagagc
     3541 aacgtgaaga attactacaa acattgaaag agcgttttga gaataatatg aaccgtcata
     3601 aaggtttaga atgggctaat gttcaagaaa ggctggatgc taatactgaa aagttgtggt
     3661 cgctcaacga aatggaacga actggtggcg aacctgatgt tgttgatttc ggtaaagaaa
     3721 aaggcgaata tattttttat gattgttcag cagaaagtcc taaaggtcgt agaagtgttt
     3781 gttacgatct tgaagcgcta gaatcaagaa agaaacataa accagaaaat aacgctattg
     3841 atatggcaac tgccatgggt attgaactat taacggaaga agaatatcgt acattacata
     3901 atcttgaaaa tttcgatatg aagacgtcga gttgggtgca aacaccttct gatattagag
     3961 aacttggcgg tgcccttttt tgcgattatc gctttggaca tgtctttgtg tatcacaatg
     4021 gagcagaatc ctactatgcc gcaagaggtt ttcggggttc gttaagagtc taaaatttgt
     4081 acaggcaggt aaatttgaag tagagaatag atttgcttag ggaatagtag tgattgcata
     4141 ctttagctac ggcttcgatt ttgaggccgc aaagaatagg gctaatcaaa ccttattgta
     4201 aaagaatgaa tcaactaaaa atattaagag tatgttttga taattttctt cataaacata
     4261 ctctttttta gaatcattta ccaaaattat tagttttaat taacgtataa acgagaactt
     4321 gtaaaaggta ctcttgcaat tatcaatgaa attcacccat atgaaacacc tatgatttat
     4381 attatcccgc ttttaaatga aaattttcga gcaagtataa agtgaatgat taggaggaat
     4441 acaaaatgaa aagtaaattc catcatattg tacgagctat catgataaaa gatgaaaaat
     4501 tactagttgc tgaatacata ggccatcact attttttacc cggtggtcat gttgaaattg
     4561 gcgaatcagc agagaatgca ctaataagag agttacgaga agaacttgga gtaacttaca
     4621 gtataaaaca atttttaggc gtcatagaaa atcaatggca agccaaagaa accctccacc
     4681 atgaaattaa ccacattttt gaaatagatt caaatcaata atgccgacac cttcagtaaa
     4741 agaattacta gaaaaaaaat taagtgatga actattaact tgctggatta gtaattttta
     4801 attgtctcta tttatattaa ttccgtccac taaaagaata taaagaaaat aggctcataa
     4861 acaaggtaca tcacctagtg taaagtcata ttttgttgat gtacgcattt agtctatagc
     4921 cttatttcta ctttattttt aaaagtaaat aaactcgaaa atttggacgg aatttacatt
     4981 tgtaaatgga gggtttcata atgaatgagt ttcaacaaga actacaagcg ttaaacctta
     5041 atgcttatca acctggtaat gttgtgtatt gggatcagca acaaaatcaa tatccatatt
     5101 actacattca agacgatgca cgtcgttgtg gcggatgcgg tggcggcgga cgttgtggcg
     5161 gttgtggtgg tcgctgtggt ggttgtggcg gcggacgttg tggcggttgc gtaggttgtg
     5221 taggctgtgt tggttgtttc agctgttcta attgctggaa ctggtggttt atttaagtat
     5281 tatagatcta taatatttca catgaaagaa aagtgcagat gcaaagttat aatgtattga
     5341 aacttttctt tcatgctgtt atttttatat gttttagggg gatatttatt tgtacaaata
     5401 tgaaaaatta atttcatggg tagaaagcat taaagagacc aatcaaagtt ctgcggcagc
     5461 actttgtatt attaaagata ataagattgt actagagcat tatagtggaa cgcattcaaa
     5521 tactcctact agtaaaaaag taactatatc ttcacaattc aatgtcgctt ccgcaagaaa
     5581 aagctattta ggattaatgg tagcttatgc agtttatgag gggaaaataa aatctcttga
     5641 tgacgaggct gtaaattatt ttaaagaata tgatccttca ttacttggaa agacaacgat
     5701 aagacattta gtaactcatt cacacggttt aaatgaaacc gatgatggga cagcttttcg
     5761 tgaatttgag ccgggacaag cttgggcgta cagagatatt aatgtaagaa tggtgacgca
     5821 gcttatttat caactgtata ataagagttt tcctgaacta ttaaaggagc gtgtattcac
     5881 acctgctgat ttcaaaaaaa ctggatggag aataaaacaa gatgataatt tagttaaggt
     5941 tgttgtaaac ccagatgaag atgcaataag tagtgttggt gcagtagatg acggctccga
     6001 gaaaaatctt tttgtctctg cgagagaatt tgcattttgg ggtaaccttc atttaaataa
     6061 agggttttta aatggtaaac aaattgtgcc aaaagaagtt atagcacttg ctacgagctt
     6121 acaaagtcca gagtatacaa ataaagaact acctcaaaac gggttgtttt ggttcgttca
     6181 aaatgaacct gctttattta gcgaacttgg tgagcgagtt cctaaaggtt cctatcaaat
     6241 attaggaatt acaggaccaa cgatattagt cattccagaa tataatgtcg tcgttgctaa
     6301 aatgtacaat aaaagataca actatggtgg ggataattat ttatattatt tacgtgaatt
     6361 tagcaattta gtcgctgata catttagtag cagtaaaaat actatggtta gggtataact
     6421 actcctataa taaaaaggag tgaactttaa tgaaaaatga agtgaataaa aataaaaagc
     6481 aaaatataaa ggatgtaaac acagaacaaa acgcgattta tagcgatccg aaagatgcag
     6541 ctaatatgca aactgtaccg cagccgaaag actttgagga aattgaatat taattttctc
     6601 tccgacttat ttatgaaaga aaaatacgtc ttaagtctca tcactttcag caaattattt
     6661 gataacatat atacataagc attcgaaaat gtcttatctt aacaaagctt tctttaaatg
     6721 ctgtatataa cctacaaaaa ggcacttctt gatgaagtgt ctttttgtgt aagctaactc
     6781 aatttccatt ttccgtacag tatcttgcaa tttatcatgg atatactaac tgtgaaaatt
     6841 catgtcatga taaaagaagg ggacagttag catgaaacaa acatctataa atccattgct
     6901 aattgttcta ggtacaatca ttgttcaaat tggcctagga acaatttaca catggagttt
     6961 atttaatcaa ccccttgtaa gtaagtttgg atggaacctc aattcagtcg cgatcacgtt
     7021 ctcaattacg agtttttctt tatcattctc aactctattt gcaggaaagc tacaacaaaa
     7081 atttggactt cgtaaactta tcgctactgc aggaattgtt ttaggactcg gtttaatact
     7141 tagttcacaa gtttcctcat taccattact ttatttatta gccggtgttg tcgttggata
     7201 cgctgatgga acagcatata ttacatcact atctaattta attaaatggt ttccaaaccg
     7261 taaaggactt atttctggta tatctgtttc ggcatatgga atgggaagct taatctttag
     7321 atatgtaaac ggaaatctta tcaacagtct tggagtatca caagcatttt tatattgggg
     7381 tattatcgtc ttactattag tgttgatagg atcattcttc ttacgtgaag caattgtaag
     7441 taacactgca actgaaacat tacacaatga ttatacgccg cgtgaaatga tgaaaacgaa
     7501 acaagtatat ctcttatttt tcatgttatt cacatcatgt atgggcggac tgtatttaat
     7561 cggtatggta aaagacatcg gggtgcagct cgttggtctt agtgcagcga cggccgctaa
     7621 tgccgtcgca atgattgcaa tctttaatac aataggtaga attattcttg gaacgttatc
     7681 agataaaata ggtcgattaa aaatcgtctc ggctacattt attattattg gattatccgt
     7741 cttcacttta agttttattc cactaaatta cggaatttat tttgcttgtg tagcaagtgt
     7801 tgccttttgc tttggtggta acatcactat cttcccagca attgtgggag acttcttcgg
     7861 attaaaaaat catagtacaa actacgggat tgtttaccaa ggtttcggat ttggtgcact
     7921 tgcaggatca ttcattggag cactacttgg cggatttcag ccaaccttca ttacaatcgg
     7981 tgttctatgt gttatatctt tcgttatctc tatagtaatc cgccctccaa atgcagaaaa
     8041 gaagaaggaa atcaaatcgt taaatcgaaa aatagcttaa tgctaagaaa gagtccttat
     8101 actagggctc ttttgcttta caaaaaaatg tataaacaat gaatgacatt caaaaaatct
     8161 aattttttcc ctgtacttat ttcataatct aacattaaag aagataggaa attccgatta
     8221 ttaaattgaa taaataatgt aaaggggcgt gatagttaaa atgcaaatag taaagcaatg
     8281 ggtacaagaa gatagtgatt acataagaga aaaagtaatt gaatataacc aaaaacaact
     8341 ttcaaatgaa gaaaaaacac cttcagaaaa gattagtttt atagtgaaga atgaaaaaga
     8401 agaaatagta ggagggataa cggcaatcac attttggcac catgtatatg ttgacttcct
     8461 atgggtttct gaagaataca ggcatgaagg ttatggaagc aatttaatga atcttattga
     8521 agaatttgca ataaaaaaag aatgtaggct aatcaaatta gatactttta gtttccaagc
     8581 ccctaatttt tataagaagc atggatataa agtgataggg gtaagtgaag attatcctaa
     8641 agggcataat cattactatt tagaaaaaag gttataaatg aatcattaaa gagtgtatta
     8701 tgattaatct ttttcaaaat atgctctttt cttttgtgtg tctatgaaca cgatatcgtt
     8761 tcgtattagt tatttactca aattactttt tatgaatcaa aacaatttaa tgatagaata
     8821 aaataaagac agaaaatttt caccattcaa gggggttaca tatgaaaaaa gtaaatgtta
     8881 catacgcact tctatatgat gaaactcatg aaaagctatt aatggttaaa aacaaagggg
     8941 aaaatggttc ttattacact ttaccaggtg gagcagttaa attcggggaa acgttagaag
     9001 aggcagcaat tcgtgaagtg aaagaagaaa cagggctaga catatccgta aaaggggttt
     9061 gttccattag tgaagccttt tttgaggaga gaggacatca tgcaatcttc tttaattttt
     9121 taggagaaat aaccggaggg gaaatctgta tatcacggcc aaaagagatt gaggagatta
     9181 cctggatgga attagattca gcagaacctt atttacgtat accagaacac ttaaaaagcc
     9241 ttttacaaaa gaaagaaaca gttccttata tttttaatgg aactattatt catcaatctt
     9301 cataaagata gtatttcatt taaggcgggg gaaaaatgga gtttatcgtt aatcacaaac
     9361 aatttacaca agctctttca gaagtaagta aagctatctc aacaaaaact ttaattccta
     9421 tattatccgg aataaagata acagcagatc aatctggaat tactttaatc gcaagtaatt
     9481 cgaatatttt cattgaaaaa tttataccta tttcaattga agacgaacaa attacaacta
     9541 ttttaaaagc aggaagtatt gttgtaccag caaagtattt cattgaaatt attaagaaaa
     9601 tgccaagtga gattttaata aaaagtatga atgagcagtt gattacaatt caatcagatg
     9661 aaattacttt aaacttaaat ggactctcgg caaatgaatt tcctaatgta ccatttttag
     9721 actcacatac agaaatacaa gtagaaacgg aacagctgat tgaggtattc aaacaaactg
     9781 tctttgcaac tgctaaaaat gaatctagac cagttcttac tggcgtacat ttcgttattg
     9841 atcataacaa attaatttgt gctgctactg attcgtacag actggcttta cgtgaaatac
     9901 ccatttcttc acatgcgaaa ttaaattgca ttgtaccaag ttccactatt agtgaactac
     9961 taaaattaat gagcagtaat tcaaatttag tatacatata tctttcagag agtcacatta
    10021 tttttaaatt cggtacaatt acgttgtatt caaggcttat tgaagggaaa tatcctaata
    10081 tatccggcct aattccaaat gaatcccaaa cgattattaa tatagataga aaaaagattt
    10141 tacaaggtgt agatcggtca agtttattgg ctagtgaatg ggcaaacaac aacgttaact
    10201 tagaaatcat cgacgaatgc acaatacaaa tttcttccaa cgcttctcaa attggtaaaa
    10261 tatccgagac acaaca
//
