LOCUS       NZ_CP035996            10822 bp    DNA     linear   CON 25-JUN-2024
DEFINITION  Bacillus mycoides strain BPN36/2 plasmid p539.
ACCESSION   NZ_CP035996
VERSION     NZ_CP035996.1
KEYWORDS    RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 538903)
  AUTHORS   Fiedoruk,K., Drewnowska,J.M. and Swiecicka,I.
  TITLE     The dynamics of pangenome and proteome of selected Bacillus cereus
            sensu lato representatives from the primary and anthropologically
            changes environments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 538903)
  AUTHORS   Drewnowska,J.M., Fiedoruk,K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Department of Microbiology, University of
            Bialystok, 1J Ciolkowskiego, Bialystok 15-245, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 168.724x
            Sequencing Technology :: Illumina MiSeq and MiniIon Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018739445.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 04:17:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,019
            CDSs (total)                      :: 5,865
            Genes (coding)                    :: 5,631
            CDSs (with protein)               :: 5,631
            Genes (RNA)                       :: 154
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 100 of 234
            Pseudo Genes (incomplete)         :: 149 of 234
            Pseudo Genes (internal stop)      :: 70 of 234
            Pseudo Genes (multiple problems)  :: 74 of 234
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:36:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 331449
            Orig. end                         :: 342271
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035996.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10822
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10822
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10822
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5822](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5822)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(102..120)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.66"
                     /tool="antismash"
     misc_feature    104..118
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.98"
                     /tool="antismash"
     misc_feature    complement(105..120)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 23.66"
                     /tool="antismash"
     gene            180..530
                     /locus_tag="EXW32_RS28805"
                     /old_locus_tag="EXW32_29480"
     CDS             180..530
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473477.1"
                     /locus_tag="EXW32_RS28805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_29480"
                     /product="hypothetical protein"
                     /protein_id="WP_215586238.1"
                     /transl_table=11
                     /translation="MFKKIIVSSMALTMFGGAATMLETPTAQAATYNSASSYFDVTPEE
                     AAAFYQSQDYKKLKVFMDDFEARNPYTPGGGYAQGSVRMQRFNDALRDAKLTNVYQEFI
                     RISYYNHYPEPK"
     gene            complement(876..1091)
                     /locus_tag="EXW32_RS28810"
                     /old_locus_tag="EXW32_29485"
     CDS             complement(876..1091)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167046.1"
                     /locus_tag="EXW32_RS28810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_29485"
                     /product="alpha/beta-type small acid-soluble spore protein"
                     /protein_id="WP_001167047.1"
                     /transl_table=11
                     /translation="MQIRENNNSNEILIAAAASAIEQMKYEIAQEFGVTLGPDSTARAN
                     GSVGGEITKRLVRMAQEQLSGQYKIH"
     misc_feature    complement(1466..1480)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 20.1"
                     /tool="antismash"
     gene            complement(1728..2141)
                     /locus_tag="EXW32_RS28815"
                     /old_locus_tag="EXW32_29490"
     CDS             complement(1728..2141)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002204073.1"
                     /locus_tag="EXW32_RS28815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_29490"
                     /product="DUF86 domain-containing protein"
                     /protein_id="WP_215586239.1"
                     /transl_table=11
                     /translation="MKNEVILNKISTIERCIKRIQDVYGNAPENLEDFTKQDSIILNIQ
                     RACEASIDLAMHIVAGKKLGLPQSSREAFDLLVTADLLSAELANKLKAMVGFRNIAVHD
                     YQSVNLDIVRQIIEKHLKDFKLFTKEVMGILDL"
     gene            complement(2131..2538)
                     /locus_tag="EXW32_RS28820"
                     /old_locus_tag="EXW32_29495"
     CDS             complement(2131..2538)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016136174.1"
                     /locus_tag="EXW32_RS28820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_29495"
                     /product="nucleotidyltransferase domain-containing protein"
                     /protein_id="WP_215586240.1"
                     /transl_table=11
                     /translation="MIFQSMITAIIEKLQETVNPYWIIVFGSTAENREREDSDIDIAYL
                     SDTTLGNYERFLLAQELANIVHRDVDLVDLSEASTVFQAQIIHTGKTIFCSDDKRKVVF
                     EMKTLKMYSKLNEERQVVFDDIKKRGSIYEE"
     misc_feature    2494..2510
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 25.92"
                     /tool="antismash"
     gene            complement(3149..4129)
                     /locus_tag="EXW32_RS28825"
                     /old_locus_tag="EXW32_29505"
     CDS             complement(3149..4129)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EXW32_RS28825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EXW32_29505"
                     /product="hypothetical protein"
                     /protein_id="WP_215586241.1"
                     /transl_table=11
                     /translation="MELKVVNPSIKSYQFIAFPLAVIEQHDESKKWLFNNFIQLAYDNQ
                     KNSPVPFCFYMLDYTINPFLDVMKFTREIKNLVNLNIIEFTQKAIENNYYLYLNVDEYY
                     IPNRKSYTNQHYSHDILVYGFDKNKKVFKVLGYDNTMKFAPSEVPYEKFANAFTSLDKI
                     NNDCEQVCLFKPKIEPYEISGSIIKERLIDYIESKNTSQQYADIREPWERSYGLSAYTS
                     LKAYFESFKRNSLEKIDPINSLIILEHKRLMRDRVNFLSSNGFLKERIDLLSFEELEKL
                     SETIHLLCLKFNIKRDISIIDNIVNIMNVLKDKEEQTYSKLIELL"
     gene            complement(4258..5001)
                     /locus_tag="EXW32_RS28830"
                     /old_locus_tag="EXW32_29510"
     CDS             complement(4258..5001)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EXW32_RS28830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EXW32_29510"
                     /product="hypothetical protein"
                     /protein_id="WP_002068812.1"
                     /transl_table=11
                     /translation="MKDVNYLISKLVTDKELFTQFKKEPMEVMQILQIDVSKRKMLLNL
                     DMNNVGVFAYGAFVKRFSKLRSYIPITLRALRILDIENAVFFEEVHKLRSKKAFQFPPL
                     PGDLEACIEVMEENNYPPFAIDILQFESAIVLASVRKNITPCRKPEYNFDNSSLNKISI
                     SKNVSLINVRYDVYQLRNSILSNQISSLEQSESYYLVYKQNHRIKVKRIKLELANFILK
                     KDKFDFTNVVEDLKELYSKGILSIN"
     gene            complement(5001..5822)
                     /locus_tag="EXW32_RS28835"
                     /old_locus_tag="EXW32_29515"
     CDS             complement(5001..5822)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /locus_tag="EXW32_RS28835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_29515"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_215586242.1"
                     /sec_met_domain="DUF692 (E-value: 5.6e-71, bitscore: 233.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGIGIGLRHEMASDIIKNRDCIDWLEFYPDSFIHQEADITLLEE
                     VKEMFPLCPHCLNFSIGSKERINKEYLNSIKKMVNAYNSPWFSDHLCFTQVNGVDVGHL
                     APIERSEESLEIVTRNIKTIKKEIKKEFLIENITFDFELPTNQYNEMEFINKVLGNADC
                     GLLLDVTNVFINSENHKFDPYKYIDEINLERVMEIHIAGGIKDSQGYWIDSHSERINKE
                     VWNLLEYTLRKSSTKGILLELDANYPKHFEVVREELKIAKNLFNKYNCAVK"
     gene            complement(5837..7699)
                     /locus_tag="EXW32_RS28840"
                     /old_locus_tag="EXW32_29520"
     CDS             complement(5837..7699)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /locus_tag="EXW32_RS28840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_29520"
                     /product="S8 family serine peptidase"
                     /protein_id="WP_215586243.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 4.2e-17, bitscore:
                     57.3, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVKVAVIDSGVEYEHFDIISEKISGINLLNQKKELGDISDQLGHG
                     TAICSILSKSDDITSIQVLKIFNNDYRVDLDKLIEAIEYAIYLNVDIINLSLGILQHDN
                     LMSLKIACQKAVEKGIVIVAACENSGEISYPAEWDFVIGVDTNKNVNKVDEFFFLENSS
                     IEIQAFGKPQKVAHLKSRHIVVNDNSYATPHITKLIAKIINKHGRKNIESIKKLLMKQA
                     KQTIDLYQVTSEELVLKAKQFETDFDSLRNKISSNELDVNKAVMFPFIKEFHPFLIFPE
                     LSTFKIKDIYDLRQLGKIGSSTKKYVPLSNEELIIKDITTIDMQADFDTLILGYTNELI
                     DKVGKSVFVNLIDLCIENNKQIFFLDDFFELDFMKNKDISNVTYPSLGLKELNLYSLYR
                     GKISNHSKPIIGVFGTSSSQGKFTTQLWLRKMFLEKKYNVGQLGSEHQSILFGFDKVFP
                     YGYNANINLPFEFYVTMVNNLIHQIETEKDPDLIMLGAQSGTIPYSLNTLGNFTYPTLA
                     LLQGANPDLVVLHINTFDDLVYIEKTIKTIESLSNAKVVALVLYSAEKVVTPSGRVKTK
                     FIEDPNKINTLKEIISKNLNLPVYDTTKLKERESLFNDIISFFS"
     misc_feature    complement(7756..7771)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 23.4"
                     /tool="antismash"
     gene            complement(7819..7953)
                     /locus_tag="EXW32_RS30245"
     CDS             complement(7819..7953)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EXW32_RS30245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_286675264.1"
                     /transl_table=11
                     /translation="MKKTLKKPTKVGKKVEETKVTLYMGELGCGTGCTGGVLGRTRGW"
     misc_feature    7938..7953
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.73"
                     /tool="antismash"
     gene            complement(8024..8158)
                     /locus_tag="EXW32_RS30250"
     CDS             complement(8024..8158)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EXW32_RS30250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_002068805.1"
                     /transl_table=11
                     /translation="MKKALKKPTKVGKEVEETKVTLYMGELGCGTGCTGGVLGRTRGW"
     gene            complement(8163..8402)
                     /locus_tag="EXW32_RS28845"
                     /old_locus_tag="EXW32_29525"
     CDS             complement(8163..8402)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EXW32_RS28845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EXW32_29525"
                     /product="phosphopantetheine-binding protein"
                     /protein_id="WP_033707354.1"
                     /sec_met_domain="PP-binding (E-value: 1.4e-05, bitscore:
                     20.5, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKSLIGEKVDVLLQEFNIDVENGDDISDYIDSLIFMQIILELED
                     SFNITVKDEELDYNIVLSKVTIIDFIIQKQLERG"
     gene            complement(8395..10059)
                     /locus_tag="EXW32_RS28850"
                     /old_locus_tag="EXW32_29530"
     CDS             complement(8395..10059)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /locus_tag="EXW32_RS28850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_29530"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_215586244.1"
                     /transl_table=11
                     /translation="MYYKYLKKNHLYKFFIMAAIVTLFTMASRLIMPMSNQLLIDEALL
                     DKNTDSLTKAVCIFLGAMISMTVLENIKQYFLAVGENNWAAELRLSMHKFYRENYINYN
                     DENRGELLSKYTQDVECLKDKLRHIINIAVDAIQLLVIIILFFIVNVKMGMIVLICMPL
                     YFILPIFVGKVIKKKSDKVQNQKGILMRELNESFRISKDIRINNKNNWDISNLGNVFYG
                     LVKPIVSLEVWKTSMLYTSILYFIVNAVFLVVGGNMVIAGELTPGYLIAMVTYINFMNA
                     PVSNMMNNLALYKECMGADKRINEFLNIQKFEDGTNIFDYSKPFDILLQDVSLQKEHKL
                     ILKNINLAIHSGSFIFLTGKSGAGKSSLAKILSRLVNPTDGRIYIQNKESSNYEMKHFY
                     EAVSIVQIENELPNLSIKENLFFHLSKKEINKIYIDDYKEIMILLKIPEFITNFENGLD
                     TIVFSNKLSTGQKQRINLFRAIVKNSPILILDEATSGLEFELEKEIYEYLKEHRKNLIT
                     IIITHRINLITPEDLVINVNNGVIENENEYERGRKNG"
     misc_feature    complement(10299..10310)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.81"
                     /tool="antismash"
     gene            complement(10488..10667)
                     /locus_tag="EXW32_RS30420"
                     /old_locus_tag="EXW32_29535"
     CDS             complement(10488..10667)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928044.1"
                     /locus_tag="EXW32_RS30420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_29535"
                     /product="hypothetical protein"
                     /protein_id="WP_350457259.1"
                     /transl_table=11
                     /translation="MMLFFSIILIGVVISLRVIALNMIHRQEIEAKYVYCSNCNRKIRK
                     GNSAPYCSKCNLFF"
ORIGIN
        1 atagtatata tccctctttc aatctattaa tttcctgtta cattccttca tatagagatg
       61 gagggacttc tcgttcacat cataaattat gcaccattgc atttgataac cattttcatt
      121 gtaactataa tgattacaag attactatta atccaaatat tctacgtgga ggttttatta
      181 tgtttaaaaa aatcattgtg tcttctatgg cacttactat gtttggtgga gctgcaacta
      241 tgctagagac acctactgct caagcagcta cttataactc agcatcttct tactttgatg
      301 ttactcctga agaagccgca gctttctatc aatctcaaga ctataaaaaa ttaaaagtat
      361 ttatggatga ctttgaagct agaaatcctt atactcctgg tggaggctac gctcaaggta
      421 gtgtacgtat gcaaagattc aacgatgctc ttagagatgc taaattaaca aatgtctatc
      481 aagaatttat tagaatctca tattacaatc attatccaga accaaaataa tacaaatgta
      541 tagaatatct cttattgatt taactttgcc caattatctt atctcctaat tttaaatgta
      601 taatccaaaa ataattatta gaaaccattc ttttaaggat ggtttttcta ttttccctga
      661 aaattagcat atgaatgcta atttatctcc atataaaaca ttaaatgaat taactactct
      721 actgtgggaa tacatctaaa aagttaactg ccatcaactg agcaacgtta gccatttatt
      781 tgatttaaac actttaccta tacacgataa aacaggagtg tgaataatac tttcacactc
      841 cttccctatc ttttggctca aacacatatt ttctcttagt gaattttata ttgtcctgat
      901 aactgttctt gcgccatacg cactaagcgt tttgtaattt ccccacctac tgaaccattt
      961 gctcttgctg ttgaatctgg tccaagtgta acaccaaact cctgagcaat ttcatacttc
     1021 atttgctcaa tagcacttgc tgcagctgct atcaatattt cgtttgaatt attattttcg
     1081 cgaatttgca tttcttacac acctccattg attttggtaa tatcatgctc tatttcctgt
     1141 aaaatatgca aaaaatattc tttaaaaaat acatgctatt atattagtac aaagtcattt
     1201 gttcccttat ccgaaaacca aagtaaaaac cctgtactcc ttgacagagt ttttaccttg
     1261 gacagattca ccgtttttta taaatttaca gaaacatatc tttcctcagg ctatttgatt
     1321 acgtggaaat cccctattgc atgtggatat ttattcctat cctttactct aacgattgca
     1381 ctaatatcat tcaaacataa ataaacttta atatacgaat ttaccatatt gtattctcct
     1441 ttttttaatg ttatatttat atatgatcga taaacttgat tttcttctta ttcaaattta
     1501 atagttacat cttcttaaac agcttgtata ttttaaaaca aaaaagacac cttaaagtgt
     1561 cttttttcaa tatgtccatg taatatttta tttgttaaag ctagtaaaga atccacagag
     1621 gtactttctt cctcctaaaa ttagaagcag gataacatcc ttccacctca tgatatacag
     1681 ttgctacaat tttctcctat gtaatcatgt ttctgagatt tgtgcttcta taggtccaga
     1741 attcccataa cttctttcgt aaacagctta aagtccttta gatgtttttc aataatttga
     1801 cgaacaatat ctaaatttac actttgataa tcatgaacag cgatatttcg aaatccaacc
     1861 attgccttta acttatttgc tagctctgcg ctaagtaaat ctgctgtaac cagcaaatca
     1921 aaagcctctc gactcgattg aggtaatcct aattttttcc ctgcgacaat atgcattgct
     1981 aaatcaatac tcgcttcaca agctctttgt atgttcaata ttattgaatc ttgttttgta
     2041 aaatcctcta aattttcagg ggcatttcca tacacatctt ggattctttt tatacaacgc
     2101 tctattgtag aaattttatt taaaataacc tcattcttca tatattgaac ctcttttctt
     2161 tatatcatca aatactacct gtcgttcttc attcaatttt gaatacattt ttaaggtttt
     2221 catttcaaaa acgaccttac gtttatcatc cgaacaaaat attgtctttc ctgtatgtat
     2281 aatctgagct tgaaaaactg tagaagcttc ggataaatca actaaatcaa catctctatg
     2341 aacaatgtta gctaactctt gtgctaataa aaaacgttca tagttcccta aagttgtgtc
     2401 acttaaataa gctatatcaa tatcactatc ttctcgttct ctattctcag ccgttgaccc
     2461 aaacacaatt atccaatagg gattcaccgt ctcttgtaat ttttctataa tagctgttat
     2521 catgctttga aaaatcatct aattaatccc ccttagtata cttgaatttt attatatcat
     2581 aactattaat taatccttta ggctactaca aatacctaaa caacatttag ccctttttaa
     2641 tacttaagat atcacaaaat agcatgctta ttcctgaact atattaacat gagttcaaaa
     2701 ataagcatgc tatggttttg aatattaaat gattgtttat aattcaacca atttgttcgc
     2761 taaatatctt ttatattttc ctcctataga tcagtggcct atttccgtat acattctaac
     2821 aatttgatga tatgctgctc tatcaaagcc ttttttatgt taattcagtt ttaattttta
     2881 ttttaccttt gtagggggtc tcatctttct taaccccttc ccaagatttc acactttttt
     2941 cctcattgta atatatacgt aaacatgctt ctttacgaaa gtttttaatc aattagagag
     3001 gagattattt accgataaaa ggaaaaatga ttgaaccttt ttctggaaca gcaatttaaa
     3061 tcagttggaa aatcatccat gttaattaat tcatttttaa aatagatgat ttttatttta
     3121 attcaaatgt tttttgtgct aaaatcccct aaagtagttc aattagttta gaataagttt
     3181 gctcttcttt atcttttaat acattcatta tattaactat attatctatt attgatatat
     3241 ctcttttaat attaaatttt aaacatagta aatgtatagt ttcagataat ttttctaact
     3301 cctcaaaaga taataaatct atcctttctt ttaaaaatcc attagaagat aaaaaattaa
     3361 ctctatctct cattaatctc ttatgttcta aaattatcaa ggaatttatt ggatcaattt
     3421 tctctaagga atttctttta aaactttcaa aataagcctt taaggatgta taagcactta
     3481 atccgtaaga tctttcccaa ggctctctta tatctgcata ttgttgagat gtgttttttg
     3541 attcaatata atctattaat ctttctttaa taatagatcc acttatttca tatggttcta
     3601 ttttaggttt aaaaagacaa acctgttcac aatcattatt gattttatcc agggatgtaa
     3661 atgcattcgc aaacttttcg taaggaacct cagatggagc aaatttcata gtattatcat
     3721 aacctaatac tttaaaaact tttttattct tatcaaatcc atatactaaa atatcatgtg
     3781 aataatgttg attagtgtag gattttctat taggaatata atattcatcc acatttagat
     3841 aaaggtaata gttattttct atagcttttt gcgtgaattc aattatatta agatttacta
     3901 aatttttaat ctctcttgta aatttcataa cgtctaaaaa agggtttatg gtatagtcca
     3961 acatataaaa acaaaaagga actggtgaat tcttttgatt atcatatgct aactgaataa
     4021 agttattaaa tagccatttc ttactttcgt catgttgctc aattactgca agtggaaaag
     4081 caataaattg atatgattta attgaagggt taactacttt aagctccaaa ataatccccc
     4141 tatctaaaat ttttaaaaat taaacactta aaataatcac taaacattaa atgttatagc
     4201 aattatttat attgtttatt gtctatccga ctctctatca ttggattaaa tttaagttta
     4261 attaattgat aaaattcctt tactgtatag ttcctttaaa tcctctacaa cattagtaaa
     4321 atcaaattta tcttttttta gtatgaaatt tgctagttct aattttatac gtttaacctt
     4381 aatcctatga ttttgtttat agaccaaata gtagctttca gattgttcaa gtgaagatat
     4441 ttggttactt agaatgctat ttcttaattg ataaacatca tatctaacat taattaaaga
     4501 aacattttta gatatactga ttttatttaa actactatta tcaaagttgt attctggttt
     4561 tctacaaggt gtaatatttt ttctgacaga tgctaaaact atcgctgatt caaattgaag
     4621 tatgtcaata gcaaatgggg gataattatt ttcctccatc acttctatgc atgcttctag
     4681 gtcaccagga agaggtggaa actgaaatgc ttttttagac cttagtttat gcacctcttc
     4741 aaaaaaaact gcattttcaa tatcaagaat tcttaaagcc ctaagagtaa ttggaatgta
     4801 acttcttaac ttagaaaacc tttttacgaa agcaccataa gcaaatacac ctacattgtt
     4861 catatctaag tttaatagca tctttctttt acttacatca atttggagga tttgcattac
     4921 ttccattggt tcttttttaa attgtgtaaa tagttctttg tcagtaacta atttacttat
     4981 taaataatta acatctttca tcatttcacc gcacaattat atttgttaaa taaatttttt
     5041 gctattttta gttcttctct cacgacctca aaatgttttg gataattagc atctagctca
     5101 agtaatattc cttttgttga agactttctt aatgtatatt ctaatagatt ccacacttct
     5161 ttattaattc tttcagagtg ggaatcaatc caataacctt gtgaatcctt aataccgcca
     5221 gctatatgaa tttccattac tctttctaaa ttaatttcat caatatattt atatgggtca
     5281 aacttatgat tttcagaatt tatgaataca tttgttacgt ctagaagcaa tccacaatct
     5341 gcatttccca gcactttgtt gatgaattcc atttcattat attgattagt tggtagctca
     5401 aaatcgaagg taatattttc tattaagaat tcttttttta tttctttttt tatagtctta
     5461 atatttcttg ttactatttc taaggattct tcgcttctct ctattggtgc tagatggcca
     5521 acatcaactc catttacttg tgtgaaacat aaatgatcac tgaaccaagg tgaattatat
     5581 gcattgacca tttttttgat tgaatttaaa tattccttat taattctttc tttgctcccg
     5641 atagagaaat ttaaacagtg cggacataat ggaaacatct cttttacttc ttcaagtaat
     5701 gtaatatctg cctcttgatg tataaatgaa tccggataga actctaacca atcaatacaa
     5761 tctctatttt ttataatgtc actagccatt tcatgtctta gtccaatacc aattccaagc
     5821 atatactagt tccctcctaa ctaaaaaatg atatgatatc attgaacaaa ctttctcttt
     5881 cttttaattt tgtagtatcg tataccggta gatttaaatt tttagatatt atttctttta
     5941 aggtgtttat tttgtttgga tcttcaatga attttgtttt cactcgtcct gaaggggtaa
     6001 caaccttctc tgccgaatat aatactaacg ctacaacctt tgcgttggat aagctctcga
     6061 tagtttttat tgttttttct atatacacta aatcatcaaa agtgttgata tgaaggacaa
     6121 ccaaatctgg atttgctcct tgtaatagag ctagtgtagg atacgtaaaa ttacctagag
     6181 tattaagcga gtatgggatt gtacctgatt gagcacctaa cataataaga tcaggatctt
     6241 tttcagtctc aatttgatgt attaaattat ttaccattgt cacataaaac tcaaacggta
     6301 agttaatatt agcgttataa ccataaggaa ataccttgtc aaaaccaaaa agaatagatt
     6361 gatgttcgct accaagttga ccaacattgt attttttctc caaaaacatc tttcttaacc
     6421 acaattgtgt agtgaatttt ccttggctag aacttgtccc aaaaacacct attatcggct
     6481 tcgaatgatt agaaattttc cctctatata atgagtacaa atttaattct tttaatccaa
     6541 gagaaggata tgtaacattg gatatatcct tatttttcat aaaatccaac tcaaaaaaat
     6601 catctaaaaa aaagatttgc ttattatttt ctatacataa atcaattaaa tttacaaaaa
     6661 cactttttcc taccttatct atcaactcgt ttgtatatcc taatatgagt gtgtcaaaat
     6721 cagcttgcat atcaattgtt gtaatgtctt ttataataag ttcttcattg cttaagggaa
     6781 catatttttt tgttgatgaa ccaatctttc ctaattgtct aaggtcgtaa atgtctttta
     6841 ttttaaaagt agataattct ggaaagatta aaaagggatg aaattctttt ataaaaggaa
     6901 acattaccgc tttattaaca tcaagttcat tactactgat tttgtttctg agtgaatcaa
     6961 aatcagtctc aaattgtttc gctttcaaaa ctaattcctc agaagtgact tgatatagat
     7021 ctatagtttg ttttgcttgt ttcattaata attttttaat tgattctata ttttttcttc
     7081 cgtgtttatt tataatttta gcaattaatt ttgtaatatg tggagtggca tagctattat
     7141 catttacgac tatatgcctt gattttaaat gtgcgacttt ttgcggttta ccaaaagctt
     7201 gtatttctat agaggaattt tctaaaaaaa agaactcatc tactttattt acatttttat
     7261 tggtatctac acctattaca aaatcccatt cagctggata cgaaatttcg ccactatttt
     7321 cacatgcggc aacaatgaca attccctttt ctactgcttt ttgacaagca atttttaaac
     7381 tcattaagtt atcatgttgt aaaattccta agcttaaatt aataatatcg acatttaaat
     7441 atattgcata ttcaatcgct tcaattaatt tgtcaagatc taccctgtag tcattattaa
     7501 aaatttttaa aacttgtatt gaagtaatat catctgattt agacaatatt gaacaaatcg
     7561 cagttccgtg gcctaactgg tcagatatat ctcctaattc ctttttttga tttaaaagat
     7621 taattccaga aattttttcg cttattatat caaagtgttc atattcaaca ccactatcaa
     7681 ttacagcaac ttttaccatt tttcctcctt aattaacaat aaattaaaaa ggaaggtgat
     7741 acttcccttc ctttttataa taattttcaa taatgttact atgtttttat gcggatgagt
     7801 ggcaattaac gattaatatt accagcctct tgtgcgtcct aataccccac cagtacaacc
     7861 agtaccacaa ccaagttcac ccatatataa ggtaactttt gtttcttcta ctttttttcc
     7921 tacttttgtt ggttttttta aagttttttt cattgtttta cccctttcaa atttttgtga
     7981 aacaaactta atgtattttt atttatcaaa aatacattta actttaccag cctcttgtac
     8041 gccctaatac cccaccagta caaccagtac cacaaccaag ttcacccata tataaggtaa
     8101 cttttgtttc ttctacttct tttcctactt ttgttggttt ttttaatgcc tttttcattt
     8161 tttcaccccc tttcaagttg tttttgtata ataaaatcaa taattgttac tttgcttaaa
     8221 actatattgt aatctagctc ttcatcctta acggtaatat taaatgaatc ctctagttct
     8281 aatataattt gcataaatat taatgaatct atatagtcgg aaatgtcatc tccattttcc
     8341 acatctatat tgaattcctg taacaataca tccacttttt ctccaattaa agatttatcc
     8401 attctttctt cccctctcat attcattctc attttctatt actccattat ttacattaat
     8461 cactaaatct tcaggggtaa ttaagttgat tctatgagta ataattattg ttattaaatt
     8521 ttttcgatgt tcttttaaat actcatatat ttctttttca agttcgaatt ccaaacccga
     8581 agttgcctca tctaaaatta aaatcggcga atttttcact atagctctaa ataagttgat
     8641 tctttgtttt tgccctgttg ataatttatt actaaaaact attgtgtcta atccattctc
     8701 gaaattagtt ataaattcag ggatttttaa taaaatcata atttccttat agtcatctat
     8761 atagatttta tttatttcct ttttactaag gtgaaagaaa aggttttcct ttatagataa
     8821 attcggtaat tcattttcta tttgtacaat acttacagct tcgtaaaaat gtttcatttc
     8881 gtagtttgaa gattctttat tttgtatata aatccttcca tcagttggat ttactaatct
     8941 agaaagtatt ttagctaaag atgattttcc tgcaccactt tttccagtta aaaatataaa
     9001 acttcctgaa tgaatagcga gattaatgtt ttttaaaatg agtttatgtt ctttctgtaa
     9061 agagacatct tgaagtaata tatcaaacgg cttactataa tcaaaaatat ttgtaccgtc
     9121 ctcaaatttc tggatattca gaaattcatt tatacgttta tctgccccca tacattcttt
     9181 gtaaagagcc aaattgttca tcatattaga aacaggagca ttcataaaat ttatataggt
     9241 aaccattgca ataagatatc ctggagttaa ttcaccagcg atcaccatat ttccacctac
     9301 tactagaaaa acagcattca caatgaaata tagtatactt gtatacaaca tcgaagtttt
     9361 ccatacttct aaagatacaa taggcttgac taaaccataa aaaacattgc ctaaattact
     9421 aatatcccaa ttatttttat tattaattct aatgtctttt gagatacgga agctttcatt
     9481 taactctctc atcaaaatcc ctttttgatt ttggacttta tctgattttt ttttaataac
     9541 tttacctaca aaaataggta atataaagta taatggcata catattaaca ctatcattcc
     9601 catttttaca ttaacgataa aaaataaaat aataataact agcaactgaa tggcatcaac
     9661 tgcaatattg attatatgcc ttaatttatc ttttaaacat tctacatcct gagtatattt
     9721 agaaagtaat tcccccctgt tttcatcgtt gtagtttata taattctctc tataaaattt
     9781 atgcattgac aacctcaact cagccgccca gttattctct ccaacagcta aaaagtactg
     9841 ttttatattt tcaagtacag tcattgagat cattgcacct aaaaatatac aaaccgcctt
     9901 tgttaaactg tctgtgttct tatctaaaag tgcttcatct ataagcaatt gattggacat
     9961 cggcataatt aatctacttg ccatggtaaa gagtgttaca atcgcggcca ttataaagaa
    10021 tttatagaga tgattctttt taagatattt ataatacata attccccagc acccaatctt
    10081 taagaatttc tataccattt ttaaatctca caatcccaaa agatttacct cctttattaa
    10141 gagtataata ttaaaaccaa aaacagaaag cgcttacttt tatggtttga attagaataa
    10201 actatgcaaa ttaagaatga atctatgcac atgatttgga taaattgtta aagttggagt
    10261 ttctggattc aaatttaacg gtaaaaacca ctagtagcga aaacgtttgc atgaaagacc
    10321 gaattcaaac atcgagattc aatttttaaa aattagaatc atattaaaat cattccttaa
    10381 tcccaataat ataatatcag ataatattta tcaacctttt tataccattt tttcaaaaaa
    10441 aagtttattt tatcaaaaca attaaatttt aaaatgcgtc ttttgtatca aaaaaacagg
    10501 ttacatttgg aacaataagg tgcagaattt cctttcctaa ttttcctatt acaatttgag
    10561 caataaacat acttagcctc aatttcctga cgatgtatca tatttagcgc tattactctt
    10621 aaactaatta ctactcctat caaaattata ctgaaaaata acatcataat aattcctcca
    10681 tgatatattg agattgagag aaaaattata ttattcaatg aaatcctaaa tgtgtgttcg
    10741 aaaaacaaca gaaaattaag gtttatatac aatagaattc ttttaaataa acttaatttt
    10801 taaaagttaa aaatacccct ta
//
