LOCUS       NZ_CP035994            10282 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Bacillus mycoides strain BPN36/2 chromosome, complete genome.
ACCESSION   NZ_CP035994
VERSION     NZ_CP035994.1
KEYWORDS    RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5347123)
  AUTHORS   Fiedoruk,K., Drewnowska,J.M. and Swiecicka,I.
  TITLE     The dynamics of pangenome and proteome of selected Bacillus cereus
            sensu lato representatives from the primary and anthropologically
            changes environments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5347123)
  AUTHORS   Drewnowska,J.M., Fiedoruk,K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Department of Microbiology, University of
            Bialystok, 1J Ciolkowskiego, Bialystok 15-245, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 168.724x
            Sequencing Technology :: Illumina MiSeq and MiniIon Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018739445.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 04:17:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,019
            CDSs (total)                      :: 5,865
            Genes (coding)                    :: 5,631
            CDSs (with protein)               :: 5,631
            Genes (RNA)                       :: 154
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 100 of 234
            Pseudo Genes (incomplete)         :: 149 of 234
            Pseudo Genes (internal stop)      :: 70 of 234
            Pseudo Genes (multiple problems)  :: 74 of 234
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:36:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2623210
            Orig. end                         :: 2633492
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035994.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10282
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10282
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10282
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5282](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5282)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    20..34
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.81"
                     /tool="antismash"
     gene            complement(973..1416)
                     /locus_tag="EXW32_RS13090"
                     /old_locus_tag="EXW32_13425"
     CDS             complement(973..1416)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="EXW32_RS13090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_13425"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_002085308.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDETHEKLLMVKNKGENGSYYTLPGGAVKFGETL
                     EEAAIREVKEETGLDISVKGVCSISEAFFEERGHHAIFFNFLGEITGGEICISRPKEIE
                     EITWMELDSAEPYLRIPEHLKSLLQKKETVPYIFNGTIIHQSS"
     gene            complement(1600..2016)
                     /locus_tag="EXW32_RS13095"
                     /old_locus_tag="EXW32_13430"
     CDS             complement(1600..2016)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016104977.1"
                     /locus_tag="EXW32_RS13095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_13430"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_002085307.1"
                     /transl_table=11
                     /translation="MQIVKQWVQEDSDYIREKVIEYNQKQLSNEEKTPSEKISFIVKNE
                     KEEIVGGITAITFWHHVYVDFLWVSEEYRHEGYGSNLMNLIEEFAIKKECRLIKLDTFS
                     FQAPNFYKKHGYKVIGVSEDYPKGHNHYYLEKRL"
     gene            complement(2197..3405)
                     /locus_tag="EXW32_RS13100"
                     /old_locus_tag="EXW32_13435"
     CDS             complement(2197..3405)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="EXW32_RS13100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_13435"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_002168289.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAGKLQQKFGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLINSLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNTVTETLHNDYTPREMMKTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAIFNTIGRIILGTLSDKIGRLKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFQPTFITIGVLCVISFVISIVIRPPNAEKKKEIKSLNR
                     KIA"
     gene            complement(3684..3827)
                     /locus_tag="EXW32_RS13105"
     CDS             complement(3684..3827)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="EXW32_RS13105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002013225.1"
                     /transl_table=11
                     /translation="MKNEVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFEEI
                     EY"
     gene            complement(3859..4887)
                     /locus_tag="EXW32_RS13110"
                     /old_locus_tag="EXW32_13440"
     CDS             complement(3859..4887)
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002085304.1"
                     /locus_tag="EXW32_RS13110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_13440"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_016127063.1"
                     /transl_table=11
                     /translation="MYKYEKLISWVESIKETNKSSAAALCIIKDNKIVLEHYSGTHSNT
                     PTSKKVTISSQFNVASARKSYLGLMVAYAVYEGKIKSLDDEAVNYFKEYDPSLLGKTTI
                     RHLVTHSHGLNETDDGTAFREFEPGQAWAYRDINVRMVTQLIYQLYNKSFPELLKERVF
                     TPADFKKTGWRIKQDDNLVKVVVNPDEDAISSVGAVDDGSEKNLFVSAREFAFWGNLHL
                     NKGFLNGKQIVPKEVIALATSLQSPEYTNKELPQNGLFWFVQNEPALFSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSSSKNTMV
                     RV"
     gene            complement(5001..5282)
                     /locus_tag="EXW32_RS13115"
                     /old_locus_tag="EXW32_13445"
     CDS             complement(5001..5282)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001869.1"
                     /locus_tag="EXW32_RS13115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_13445"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_215586066.1"
                     /sec_met_domain="TIGR03601 (E-value: 4.2e-37, bitscore:
                     124.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNEFQQELQALNLNAYQPGNVVYWDQQQNQYPYYYIQDDARRCGG
                     CGGGGGGRCGGCGGRCGGCGGGRCGGCVGCVGCVGCFSCSNCWNWWFI"
     misc_feature    5296..5311
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 26.71"
                     /tool="antismash"
     gene            complement(5482..5928)
                     /locus_tag="EXW32_RS13120"
                     /old_locus_tag="EXW32_13450"
     CDS             complement(5482..5928)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="EXW32_RS13120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_13450"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_000836819.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAIMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELREELGVTYSIKQFLGVIENQWQAKETLHHEINHIFEIDSNELKSDFTPISKESH
                     LAFHWIDFNKENINSYTIMPTPSVKELLEKKLSDELLTCWISNF"
     gene            complement(6301..6870)
                     /locus_tag="EXW32_RS13125"
                     /old_locus_tag="EXW32_13455"
     CDS             complement(6301..6870)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002065694.1"
                     /locus_tag="EXW32_RS13125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_13455"
                     /product="DUF4256 domain-containing protein"
                     /protein_id="WP_000665071.1"
                     /transl_table=11
                     /translation="MKESNKKELSLEQREELLQTLKDRFENNMNRHKGLEWANVQERLD
                     ANTEKLWSLNEMERTGGEPDVVDFGKEKGEYIFYDCSAESPKGRRSVCYDLEALESRKK
                     HKPENNAIDMATAMGIELLTEEEYRTLHNLENFDMKTSSWVQTPSDIRELGGALFCDYR
                     FGHVFVYHNGAESYYAARGFRGSLRV"
     gene            complement(7054..7518)
                     /locus_tag="EXW32_RS13130"
                     /old_locus_tag="EXW32_13460"
     CDS             complement(7054..7518)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136577.1"
                     /locus_tag="EXW32_RS13130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_13460"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_002168287.1"
                     /transl_table=11
                     /translation="MNVQLKIVTRENWEDALKLRVKENQIHLVPAVAVSLAKVYIKPDG
                     ENVEYIPFAIYDGDLIVGFIMHAFVRESTDMYWINGFIIDEKEQGKGYGKAALQEIINL
                     IKNNFKECEEIRLTVHKDNISAKKLYECYDFKPLGNVYDGEEVYRLLLLK"
     gene            complement(7596..8519)
                     /locus_tag="EXW32_RS13135"
                     /old_locus_tag="EXW32_13465"
     CDS             complement(7596..8519)
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="EXW32_RS13135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_13465"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_002127616.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATGPLTNEITTYYPEIHGPEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGEDLTIVDVGRSTSLAIAFNLWNELMLNIKGIYFMGGVFLEAGNVTPLA
                     EANVYGDPIASQIVFQQAKNITLFPLNVTNKTVLTPSVFTYILVNTQNPFKSIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVLAISGLVNPTFLNYTSRKVTVDICGETKGQTFADFRPH
                     SKSEGARIALQLDEEKFIQDFMKIML"
     misc_feature    8769..8785
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.88"
                     /tool="antismash"
     gene            complement(8803..9714)
                     /locus_tag="EXW32_RS13140"
                     /old_locus_tag="EXW32_13470"
     CDS             complement(8803..9714)
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136580.1"
                     /locus_tag="EXW32_RS13140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_13470"
                     /product="DMT family transporter"
                     /protein_id="WP_016127066.1"
                     /transl_table=11
                     /translation="MHNKKWDFRIVCAHAFTILIWGTAFPGIRMGLEAYTPEHLTLLRL
                     LIASLILFLFSLIYKLRLPDLKYIPVFFIFGALGFTIYHIALNYGEKSVNAGPASLIVS
                     VTPILTAILASVFLNERMKLNGWIGGVISFVGIAFLSLSQGDSIQLNSGGLLILLAAIS
                     ESLFFVFQRPYLKKYGFLPFTIYTIWSGTVFMLIFLPGIYQEIVTAPIEITLSVIYLGV
                     FPTVLPYIALAYITSHASASEATSSLYLTPVTACIIAWIWLGEVPTFVSIIGGGMTILG
                     VVIAHLTFNKDDYKQIESEKVW"
ORIGIN
        1 ttgttgtgtc tcggatattt taccaatttg agaagcgttg gaagaaattt gtattgtgca
       61 ttcgtcgatg atttctaagt taacgttgtt gtttgcccat tcactagcca ataaacttga
      121 ccgatctaca ccttgtaaaa tcttttttct atctatatta ataatcgttt gggattcatt
      181 tggaattagg ccggatatat taggatattt cccttcaata agccttgaat acaacgtaat
      241 tgtaccgaat ttaaaaataa tgtgactctc tgaaagatat atgtatacta aatttgaatt
      301 actgctcatt aattttagta gttcactaat agtggaactt ggtacaatgc aatttaattt
      361 cgcatgtgaa gaaatgggta tttcacgtaa agccagtctg tgcgaatcag tagcagcaca
      421 aattaatttg ttatgatcaa taacgaaatg tacgccagta agaactggtc tagattcatt
      481 tttagcagtt gcaaagacag tttgtttgaa tacctcaatc agctgttccg tttctacttg
      541 tatttctgta tgtgagtcta aaaatggtac attaggaaat tcatttgccg agagtccatt
      601 taagtttaaa gtaatttcat ctgattgaat tgtaatcaac tgctcattca tactttttat
      661 taaaatctca cttggcattt tcttaataat ttcaatgaaa tactttgctg gtacaacaat
      721 acttcctgct tttaaaatag ttgtaatttg ttcgtcttca attgaaatag gtataaattt
      781 ttcaatgaaa atattcgaat tacttgcgat taaagtaatt ccagattgat ctgctgttat
      841 ctttattccg gataatatag gaattaaagt ttttgttgag atagctttac ttacttctga
      901 aagagcttgt gtaaattgtt tgtgattaac gataaactcc atttttcccc cgccttaaat
      961 gaaatactat ctttatgaag attgatgaat aatagttcca ttaaaaatat aaggaactgt
     1021 ttctttcttt tgtaaaaggc tttttaagtg ttctggtata cgtaaataag gttctgctga
     1081 atctaattcc atccaggtaa tctcctcaat ctcttttggc cgtgatatac agatttcccc
     1141 tccggttatt tctcctaaaa aattaaagaa gattgcatga tgtcctctct cctcaaaaaa
     1201 ggcttcacta atggaacaaa ccccttttac ggatatgtct agccctgttt cttctttcac
     1261 ttcacgaatt gctgcctctt ctaacgtttc cccgaattta actgctccac ctggtaaagt
     1321 gtaataagaa ccattttccc ctttgttttt aaccattaat agcttttcat gagtttcatc
     1381 atatagaagt gcgtatgtaa catttacttt tttcatatgt aacccccttg aatggtgaaa
     1441 attttctgtc tttattttat tctatcatta aattgttttg attcataaaa agtaatttga
     1501 gtaaataact aatacgaaac gatatcgtgt tcatagacac acaaagaaaa gagcatattt
     1561 tgaaaaagat taatcataat acactcttta atgattcatt tataaccttt tttctaaata
     1621 gtaatgatta tgccctttag gataatcttc acttacccct atcactttat atccatgctt
     1681 cttataaaaa ttaggggctt ggaaactaaa agtatctaat ttgattagcc tacattcttt
     1741 ttttattgca aattcttcaa taagattcat taaattgctt ccataacctt catgcctgta
     1801 ttcttcagaa acccatagga agtcaacata tacatggtgc caaaatgtga ttgccgttat
     1861 ccctcctact atttcttctt tttcattctt cactataaaa ctaatctttt ctgaaggtgt
     1921 tttttcttca tttgaaagtt gtttttggtt atattcaatt actttttctc ttatgtaatc
     1981 actatcttct tgtacccatt gctttactat ttgcatttta actatcacgc ccctttacat
     2041 tatttattca atttaataat cggaatttcc tatcttcttt aatgttagat tatgaaataa
     2101 gtacagggaa aaaattagat tttttgaatg tcattcattg tttatacatt tttttgtaaa
     2161 gcaaaagagc cctagtataa ggactctttc ttagcattaa gctatttttc gatttaacga
     2221 tttgatttcc ttcttctttt ctgcatttgg agggcggatt actatagaga taacgaaaga
     2281 tataacacat agaacaccga ttgtaatgaa ggttggctga aatccgccaa gtagtgctcc
     2341 aatgaatgat cctgcaagtg caccaaatcc gaaaccttgg taaacaatcc cgtagtttgt
     2401 actatgattt tttaatccga agaagtctcc cacaattgct gggaagatag tgatgttacc
     2461 accaaagcaa aaggcaacac ttgctacaca agcaaaataa attccgtaat ttagtggaat
     2521 aaaacttaaa gtgaagacgg ataatccaat aataataaat gtagccgaga cgatttttaa
     2581 tcgacctatt ttatctgata acgttccaag aataattcta cctattgtat taaagattgc
     2641 aatcattgcg acggcattag cggccgtcgc tgcactaaga ccaacgagct gcaccccgat
     2701 gtcttttacc ataccgatta aatacagtcc gcccatacat gatgtgaata acatgaaaaa
     2761 taagagatat acttgttttg ttttcatcat ttcacgcggc gtataatcat tgtgtaatgt
     2821 ttcagttaca gtgttactta caattgcttc acgtaagaag aatgatccta tcaacactaa
     2881 tagtaagacg ataatacccc aatataaaaa tgcttgtgat actccaagac tgttgataag
     2941 atttccgttt atatatctaa agattaagct tcccattcca tatgccgaaa cagatatacc
     3001 agaaataagt cctttacggt ttggaaacca tttaattaaa ttagatagtg atgtaatata
     3061 tgctgttcca tcagcgtatc caacgacaac accggctaat aaataaagta atggtaatga
     3121 ggaaacttgt gaactaagta ttaaaccgag tcctaaaaca attcctgcag tagcgataag
     3181 tttacgaagt ccaaattttt gttgtagctt tcctgcaaat agagttgaga atgataaaga
     3241 aaaactcgta attgagaacg tgatcgcgac tgaattgagg ttccatccaa acttacttac
     3301 aaggggttga ttaaataaac tccatgtgta aattgttcct aggccaattt gaacaatgat
     3361 tgtacctaga acaattagca atggatttat agatgtttgt ttcatgctaa ctgtcccctt
     3421 cttttatcat gacatgaatt ttcacagtta gtatacccat gataaattgc aagatactgt
     3481 acggaaaatg gaaattgagt tagcttacac aaaaagacac ttcatcaaga agtgcctttt
     3541 tgtaggttat atacagcatt taaagaaagc tttgttaaga taagacattt tcgaatgctt
     3601 atgtatatat gttatcaaat aatttgctga aagtgatgag acttaagacg tatttttctt
     3661 tcataaataa gtcggagaga aaattaatat tcaatttcct caaagtcttt cggctgcggt
     3721 acagtttgca tattagctgc atctttcgga tcgctataaa tcgcgttttg ttctgtgttt
     3781 acatccttta tattttgctt tttattttta ttcacttcat ttttcattaa agttcactcc
     3841 tttttattat aggagtagtt ataccctaac catagtattt ttactgctac taaatgtatc
     3901 agcgactaaa ttgctaaatt cacgtaaata atataaataa ttatccccac catagttgta
     3961 tcttttattg tacattttag caacgacgac attatattct ggaatgacta atattgttgg
     4021 tcctgtaatt cctaatattt gataggaacc tttaggaact cgctcaccaa gttcgctaaa
     4081 taaagcaggt tcattttgaa cgaaccaaaa caacccgttt tgaggtagtt ctttatttgt
     4141 atactctgga ctttgtaagc tcgtagcaag tgctataact tcttttggca caatttgttt
     4201 accatttaaa aaccctttat ttaaatgaag gttaccccaa aatgcaaatt ctctcgcaga
     4261 gacaaaaaga tttttctcgg agccgtcatc tactgcacca acactactta ttgcatcttc
     4321 atctgggttt acaacaacct taactaaatt atcatcttgt tttattctcc atccagtttt
     4381 tttgaaatca gcaggtgtga atacacgctc ctttaatagt tcaggaaaac tcttattata
     4441 cagttgataa ataagctgcg tcaccattct tacattaata tctctgtacg cccaagcttg
     4501 tcccggctca aattcacgaa aagctgtccc atcatcggtt tcatttaaac cgtgtgaatg
     4561 agttactaaa tgtcttatcg ttgtctttcc aagtaatgaa ggatcatatt ctttaaaata
     4621 atttacagcc tcgtcatcaa gagattttat tttcccctca taaactgcat aagctaccat
     4681 taatcctaaa tagctttttc ttgcggaagc gacattgaat tgtgaagata tagttacttt
     4741 tttactagta ggagtatttg aatgcgttcc actataatgc tctagtacaa tcttattatc
     4801 tttaataata caaagtgctg ccgcagaact tttattggtc tctttaatgc tttctaccca
     4861 tgaaattaat ttttcatatt tgtacaaata aatatccccc taaaacatat aaaaataaca
     4921 gcatgaaaga aaagtttcaa tacattataa ctttgcatct gcacttttct ttcatgtgaa
     4981 atattataga tctataatac ttaaataaac caccagttcc agcaattaga acagctgaaa
     5041 caaccaacac agcctacaca acctacgcaa ccgccacaac gtccgccgcc acaaccacca
     5101 cagcgaccac cacaaccgcc acaacgtccg ccgccaccgc caccgcatcc gccacaacga
     5161 cgtgcatcgt cttgaatgta gtaatatgga tattgatttt gttgctgatc ccaatacaca
     5221 acattaccag gttgataagc attaaggttt aacgcttgta gttcttgttg aaactcattc
     5281 attatgaaac cctccattta caaatgtaaa ttccgtccaa attttcgagt ttatttactt
     5341 ttaaaaataa agtagaaata aggctataga ctaaatgcgt acatcaacaa aatatgactt
     5401 tacactaggt gatgtacctt gtttatgagc ctattttctt tatattcttt tagtggacgg
     5461 aattaatata aatagagaca attaaaaatt actaatccag caagttaata gttcatcact
     5521 taattttttt tctagtaatt cttttactga aggtgtcggc attattgtat aggaatttat
     5581 attttcttta ttaaaatcaa tccagtgaaa tgctaaatgg gactctttag atattggagt
     5641 gaaatcggat tttaactcat ttgaatctat ttcaaaaatg tggttaattt catggtggag
     5701 ggtttctttg gcttgccatt gattttctat gacgcctaaa aattgtttta tactgtaagt
     5761 tactccaagt tcttctcgta actctcttat tagtgcattc tctgctgatt ccccaatttc
     5821 aacatgacca ccgggtaaaa aatagtgatg gcctatgtat tcagcaacta gtaatttttc
     5881 atcttttatc atgatagctc gtacaatatg atggaattta cttttcattt tgtattcctc
     5941 ctaatcattc actttatact tgctcgaaaa ttttcattta aaagcgggat aatataaatc
     6001 ataggtgttt catatgggtg aatttcattg ataattgcaa gagtaccttt tacaagttct
     6061 cgtttatacg ttaattaaaa ctaataattt tgataaatga ttctaaaaaa gagtatgttt
     6121 atgaagaaaa ttatcaaaac atactcttaa tatttttagt tgattcattc ttttacaata
     6181 aggtttgatt agccctattc tttgcggcct caaaatcgaa gccgtagcta aagtatgcaa
     6241 tcactactat tccctaagca aatctattct ctacttcaaa tttacctgcc tgtacaaatt
     6301 ttagactctt aacgaaccac gaaaacctct tgcggcatag taggattctg ctccattgtg
     6361 atacacaaag acatgtccaa agcgataatc gcaaaaaagg gcaccgccaa gttctctaat
     6421 atcagaaggt gtttgcaccc aactcgacgt cttcatatcg aaattttcaa gattatgtaa
     6481 tgtacgatat tcttcttccg ttaatagttc aatacccatg gcagttgcca tatcaatagc
     6541 gttattttct ggtttatgtt tctttcttga ttctagcgct tcaagatcgt aacaaacact
     6601 tctacgacct ttaggacttt ctgctgaaca atcataaaaa atatattcgc ctttttcttt
     6661 accaaaatca acaacatcag gttcgccacc agttcgttcc atttcgttga gcgaccacaa
     6721 cttttcagta ttagcatcca gcctttcttg aacattagcc cattctaaac ctttatgacg
     6781 gttcatatta ttctcaaaac gatctttcaa tgtttgtagt aattcttcac gttgctctag
     6841 tgacaactct tttttattgc tttctttcat attagttcct ccattgatgt ttttattaat
     6901 gatagtttaa gtatatataa atactgtttt ataataaaat gtttaaataa ttgcgtgaat
     6961 tgagtttgcg ataatttata tgaattagta caaccaattc tccgcaatat gaccctttaa
     7021 cggaacaact tattgtttca aaaaagtaca tctttatttc aacaacaata agcgatatac
     7081 ttcctcaccg tcatatacat ttcctaatgg tttaaaatca taacattcat atagtttctt
     7141 tgcagaaata ttatctttat ggacagttaa tctaatttcc tcacactctt taaagttatt
     7201 ttttattaag ttaatgattt cctgtaatgc tgcttttcca tatccttttc cttgctcttt
     7261 ttcatcaatg ataaatccgt taatccaata catatctgtt gactctcgta caaaagcatg
     7321 cataataaaa ccaactataa ggtcaccatc atatatagcg aatggaatat attcgacatt
     7381 ttcaccatct ggttttatgt atactttagc aagtgatact gccactgcgg gaacaagatg
     7441 gatttgattt tctttaactc gtaactttaa tgcgtcttcc caattttctc tcgtaacaat
     7501 ttttaattga acgttcacta ttttaagctc ctttaaaaag agaatttatc ttttatattc
     7561 aacggagtga aatgattccc tttttcgaca tcaacttata acatgatttt cataaaatcc
     7621 tgaataaatt tttcctcatc taattgtaac gctatacgcg ccccttcaga tttagagtgt
     7681 gggcgaaaat cagcaaaagt ttgtcccttc gtttcgccac atatatccac agtcactttt
     7741 cgggatgtat aatttaaaaa tgtaggattc actaaaccgc ttatagcaag tacatcgtgt
     7801 aataatggcc cttcaataga tggattgagt ttttggtatg ctgataaata ataatcgtac
     7861 atgggtttca taatcgactt aaatggattt tgtgtattaa ctaaaatgta agtaaataca
     7921 cttggggtta aaaccgtttt atttgttaca tttaaaggga ataacgttat atttttcgct
     7981 tgctgaaata caatttgact tgctatagga tctccgtaca cgtttgcctc tgctaatgga
     8041 gtaacattcc ctgcttctaa aaagacacca cccataaaat aaattccttt tatatttaac
     8101 atcaattcgt tccataaatt aaaagcaatc gctaacgagg tagatcttcc tacatcgaca
     8161 attgttaaat cttctccata ctttacaaga atcgcagcaa tactaccgaa attgtatata
     8221 ggaatagata acacactttc cggtacatgg atgggcccta atccttctgg tccatggatt
     8281 tcagggtaat acgttgtaat ttcatttgta agtgggcctg ttgcaccgct aattacagga
     8341 atatcttgtc tgtttgctaa ctgtaaaata tatgcggcat tatgagcggc atgtatatgt
     8401 tcaacatttc catatccagt tacgatacca acaatttcaa tttcaggatg cagtaagcca
     8461 tacataattg caaacgaatc atcaatacct gggtctccaa aaaataaaac cttctccaag
     8521 aaatgcactc ctttcatagg taagataatt atgtatatgt ggaaaaaaat taaatctata
     8581 cagttctaag caaatattaa tggtgagtaa tgtattgata aaaaagaact agcattatgc
     8641 tagttctttt gcagataaat aatctaccag tttaattaaa tctcgtacaa atattggact
     8701 aactgatttt aatatagatt tttttcacta tatttataga attgaaaacc tgttattaat
     8761 ccgaaatttt gcattcaaat tgcaaatact cgtagggatg tcttaccata ccttttcact
     8821 ctcaatttgc ttataatcat ctttattaaa tgttaaatga gcaatcacaa ctccaagtat
     8881 agtcattccc cctccaatta tcgaaacaaa ggttggcacc tcacctaacc atatccaagc
     8941 gataatacat gcagttactg gtgttaaata tagagaactt gttgcctcag aagcactggc
     9001 atgggatgta atatatgcta atgcaatata cggcagtact gtcgggaaca cacctaaata
     9061 tataacactc aaagtaattt ctataggagc tgttacgatt tcttgataaa tccctggcaa
     9121 aaaaataagc ataaatacag taccagacca aattgtatat attgtaaatg gcaagaaacc
     9181 gtactttttt aagtatggcc tttgaaaaac gaaaaataga ctttctgaaa ttgccgccaa
     9241 taatattagt aatcccccac tattcaattg aatggaatct ccttgactta acgataaaaa
     9301 agcaattcct acaaaactaa ttacaccacc gatccaacca tttaatttca ttctttcatt
     9361 caaaaaaaca gaagcaagaa ttgccgttaa tataggtgtt actgatacaa ttaaactagc
     9421 aggtccagca ttcacactct tttcaccgta gttcagtgca atatgataaa ttgtaaatcc
     9481 aagagcacca aatataaaaa atactggaat atatttcaaa tcaggtaaac gtaacttgta
     9541 tataagtgaa aacagaaaaa gaatcaaaga agcgattaat aatcgtagca aagtaaggtg
     9601 ttcaggggtg taagcttcaa gccccatccg aattcctgga aaagctgttc cccatataag
     9661 aatcgtaaaa gcgtgagcac atacaatacg aaaatcccat tttttattat gcatatattc
     9721 tctccctctc caataaacca gccattttct aattaccaat tatttctttt catcatattt
     9781 aatagttgat acaatgtctt caaccagtta atcctcgaaa aaaccaacca ctttgcattt
     9841 ttaaaatgaa tgagggagtt tttaatatgt ctactgataa aaaactgcct aaatatcggc
     9901 agataataga ctatatgaaa gaaaaaattg aatacggaga atggccgatt ggaagtaagg
     9961 ttcctagcca gcggcagtta gcgaaattat ttcatgtaaa tcgtagtact gtaataattg
    10021 cactagaaga acttatggca gatggcttaa ttcaagggaa aatcgggtca ggaacaattg
    10081 taacgaataa tacatggtca ttattagcca ccaatccccc gcccgattgg agtgaatatg
    10141 taaaggctgg tattcataaa ccaagcaagt taatggtacg agaaataaat gaagcagaag
    10201 caaataaaaa attgattcat cttagtaaag gagaacttgc acctcatctt ttcccgttag
    10261 aaactatgca attcattatg cg
//
