LOCUS       NZ_CP035994            10321 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Bacillus mycoides strain BPN36/2 chromosome, complete genome.
ACCESSION   NZ_CP035994
VERSION     NZ_CP035994.1
KEYWORDS    RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5347123)
  AUTHORS   Fiedoruk,K., Drewnowska,J.M. and Swiecicka,I.
  TITLE     The dynamics of pangenome and proteome of selected Bacillus cereus
            sensu lato representatives from the primary and anthropologically
            changes environments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5347123)
  AUTHORS   Drewnowska,J.M., Fiedoruk,K. and Swiecicka,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2019) Department of Microbiology, University of
            Bialystok, 1J Ciolkowskiego, Bialystok 15-245, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 168.724x
            Sequencing Technology :: Illumina MiSeq and MiniIon Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018739445.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 04:17:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,019
            CDSs (total)                      :: 5,865
            Genes (coding)                    :: 5,631
            CDSs (with protein)               :: 5,631
            Genes (RNA)                       :: 154
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 100 of 234
            Pseudo Genes (incomplete)         :: 149 of 234
            Pseudo Genes (internal stop)      :: 70 of 234
            Pseudo Genes (multiple problems)  :: 74 of 234
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:36:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2308691
            Orig. end                         :: 2319012
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035994.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10321
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10321
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10321
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5321](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5321
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(70..86)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.99"
                     /tool="antismash"
     gene            761..1366
                     /locus_tag="EXW32_RS11710"
                     /old_locus_tag="EXW32_11980"
     CDS             761..1366
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853587.1"
                     /locus_tag="EXW32_RS11710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_11980"
                     /product="PRK06770 family protein"
                     /protein_id="WP_215586017.1"
                     /transl_table=11
                     /translation="MKTLFKIIGIIAGMAVIGVGVTYGMLYYLNNSKPTAKKASPAAPA
                     VEVLADSNVKAEDAKILENGNYSLPNSGFNKNFKWTDENIQTALHEMAHQKAKADQKWG
                     YIFITQERIESLLEIINSNDVAQKSTYVDILERWKQGKYEKVDVDHNKIWKLQSGNLGE
                     GKGVMSEKEQKELINQVFIQKGTYSGSKLIAGEVQANK"
     gene            complement(1472..2344)
                     /locus_tag="EXW32_RS11715"
                     /old_locus_tag="EXW32_11985"
     CDS             complement(1472..2344)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016095293.1"
                     /locus_tag="EXW32_RS11715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_11985"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_098632291.1"
                     /transl_table=11
                     /translation="MEMRHVKTFCAIVKYGSFSKAAHALGYAQSTVTAHIKALENDLNI
                     PLFDRLGKKILLTKAGHQFHPYALELLTIYDKAQEIPQNSDHLEGTLAITSNESLAVYR
                     LPQLLRTYKQENPKVNIVLETNTNEQALKKLHEGETDVIFIIGESIEHNDFITCNLCNE
                     TFGWIFPAHFPSNTDPLLLLQDTQFIFTEQSCGYRPMVDRFLRKSGRNPNKTFETSNVE
                     VIKQSVMCELGISILPYIVVQESNQKEQLCFQPIETPAVIQSHVIYHKSRWISPVLQSF
                     LTLLERIEK"
     gene            2474..3517
                     /locus_tag="EXW32_RS11720"
                     /old_locus_tag="EXW32_11990"
     CDS             2474..3517
                     /EC_number="1.2.1.11"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1219: N-acetyl-gamma-glutamyl-phosphate reductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200507.1"
                     /locus_tag="EXW32_RS11720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_11990"
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="WP_215586018.1"
                     /transl_table=11
                     /translation="MSEKSYHVAVVGATGAVGQKIIALLERVTKFNIEEITLLSSKRSA
                     GKIVQFKGREIKIQEAKSTSFEGVDIVFFSAGGEVSRQYVNHAVSSGAIVIDNTSEYRM
                     AHDVPLVVPEVNAHTLREHKRIIAVPNCSALQMVTALQPIRKSFGLERIIVSTYQAVSG
                     SGIHAIHELKEQAKSMLTGEEVKSTILPVKKDKKHYPIAFNVLPKVDIFTDNDFTFEEV
                     KMIQETKKILEDPNLKMAATCVRVPVVSGHSESVYIELEKEATVAEIKEVLLNAPGVIL
                     QDNPSEQIYPTPLYAEGKRDTFVGRIRKDPDTSKGFHLWIVSDNLLKGAAWNSVQIAET
                     LVENGVI"
     gene            complement(3764..4057)
                     /locus_tag="EXW32_RS11725"
                     /old_locus_tag="EXW32_11995"
     CDS             complement(3764..4057)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002168401.1"
                     /locus_tag="EXW32_RS11725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_11995"
                     /product="hypothetical protein"
                     /protein_id="WP_233151936.1"
                     /transl_table=11
                     /translation="MCNSKPIDELTIEDLKQNPIWEWAIDEAENEECDETWVKPVETIN
                     FTEELNGSIVLGELIIHNDEKFPMMCSIHIENNEVLISSIVFITKKKMSILL"
     misc_feature    complement(4288..4305)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.83"
                     /tool="antismash"
     gene            5001..5321
                     /locus_tag="EXW32_RS11730"
                     /old_locus_tag="EXW32_12000"
     CDS             5001..5321
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002189588.1"
                     /locus_tag="EXW32_RS11730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_12000"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_033730069.1"
                     /sec_met_domain="TIGR03601 (E-value: 2.1e-33, bitscore:
                     112.9, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNFQQELQALSLNDYQPGNIVYWDQQNQYPYYYITDDARRCGGC
                     GGGRCGGCGRCGGGRCGGFRCFGCFGCFSCFGCGGCSNCSNCSNCSNCFDGFNGTTVTF
                     EY"
     gene            complement(5630..6532)
                     /locus_tag="EXW32_RS11735"
                     /old_locus_tag="EXW32_12005"
     CDS             complement(5630..6532)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614455.1"
                     /locus_tag="EXW32_RS11735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_12005"
                     /product="LysR substrate-binding domain-containing protein"
                     /protein_id="WP_087974007.1"
                     /transl_table=11
                     /translation="MISIELRQLEYFLAVSKELHFTKAAEKLNISQPSLSQQIRALEHE
                     VGMPVFDRIGKKISLTEAGRVLLSHSKTIFHEVEQARSAIQDLNGLHHGSLTIGSLLTV
                     VNYLLPPAILDFNKQYPNIELSVLGLRTGEIREKLLQNELDIGITFLPVQDKEIVSIPL
                     YKSELTLVVPTSHSLTERKYVSIEELQDYPLILLPKNFYLTELITSHCQNFNFKPKPIL
                     EISTMESLIQMVAQGMGITVLPKPYIDFLQNEHIQAIKIENPTPTIDIGLIYRRDKYMC
                     AATRKFIEQLKITVHSLQK"
     gene            6662..7987
                     /gene="gabT"
                     /locus_tag="EXW32_RS11740"
                     /old_locus_tag="EXW32_12010"
     CDS             6662..7987
                     /EC_number="2.6.1.19"
                     /GO_function="GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (34-374). E-value: 1.4e-122.
                     Score: 400.9. Matches aSDomain:
                     nrpspksdomains_EXW32_RS11740_Aminotran_3.1"
                     /codon_start=1
                     /gene="gabT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200502.1"
                     /locus_tag="EXW32_RS11740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_12010"
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_215586019.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.2e-132, bitscore:
                     438.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKSSVLPGSYSQVLQKRYEEAVPTGIYHLTPLYVKEARGAIITD
                     VDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAESFTHPCFHVTPYENYITLAEKINEK
                     VPGTSKKKTMFANSGAEAIENAVKIARKVTGRSKVISFERGFHGRTLMTMSLSTKETPY
                     KAGFGPFVSDMHILPYPYYYRAEEGVSPEEVDSQVIYQFEKFLEEVSPEKVAAIVMEPV
                     QGEGGFIVPSISFVKRLREICNQYGILMIMDEVQTGFGRTGKWFATEHFHIEPDIMTLS
                     KSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGSPLSCAAGLAVFEIMEEENLVERAQQ
                     IGNRIMSHLHQMQLKYEMIGDVRGIGAMVAMELVTNRITKEPAKELTVKLIEDFWKNGL
                     ISIGAGIFDNVLRFLPPLVISNEEIDRGFEIINESFEKLCRK"
     aSDomain        6764..7783
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EXW32_RS11740_Aminotran_3.1"
                     /evalue="1.40E-122"
                     /label="EXW32_RS11740_Aminotran_3.1"
                     /locus_tag="EXW32_RS11740"
                     /protein_end="374"
                     /protein_start="34"
                     /score="400.9"
                     /tool="antismash"
                     /translation="VKEARGAIITDVDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAE
                     SFTHPCFHVTPYENYITLAEKINEKVPGTSKKKTMFANSGAEAIENAVKIARKVTGRSK
                     VISFERGFHGRTLMTMSLSTKETPYKAGFGPFVSDMHILPYPYYYRAEEGVSPEEVDSQ
                     VIYQFEKFLEEVSPEKVAAIVMEPVQGEGGFIVPSISFVKRLREICNQYGILMIMDEVQ
                     TGFGRTGKWFATEHFHIEPDIMTLSKSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGS
                     PLSCAAGLAVFEIMEEENLVERAQQIGNRIMSHLHQMQLKYEMIGDVRGIGAMVAMELV
                     "
     gene            8002..8691
                     /locus_tag="EXW32_RS11745"
                     /old_locus_tag="EXW32_12015"
     CDS             8002..8691
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262982.1"
                     /locus_tag="EXW32_RS11745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_12015"
                     /product="MOSC domain-containing protein"
                     /protein_id="WP_002168398.1"
                     /transl_table=11
                     /translation="MTKVYEILSLNLGLPKEVMYGGKLIQTGINKKQVKEPVFLSFLKF
                     NGDGQADLVHHGGVDKAVCVYTGDHYPYWEKELNQELVYGAFGENITVSGMREEDVCIG
                     DTFQLGEAIVQVTQPRQPCFKLAKKYNIPKLPLYFQDTGYTGFYFRVLKEGWILPVDTL
                     KLLKLDPKGVSILFANRIMHKEKQNSEGLKRILEVNALSTSWRKTFEKRVKGEEISTKE
                     RLEGTKE"
     gene            8954..9838
                     /locus_tag="EXW32_RS11750"
                     /old_locus_tag="EXW32_12020"
     CDS             8954..9838
                     /EC_number="2.3.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526760.1"
                     /locus_tag="EXW32_RS11750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EXW32_12020"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_002031966.1"
                     /transl_table=11
                     /translation="MGNVEKMANFLLEEPRREQLFPLFEEVFGITSQTLQDFSEKGYWD
                     YTYKALSFLQDDNAIANVAAFSLPLLINGEKINATGIQSVMTHPNYRRRGLMKQLFSKM
                     IEEIDEQCECAVLFTENPELYKPFGFQIVQEYLMTVSYENKGYKDSQLKKLDFYNEEDR
                     QLIHEIIENGQRLSNKFSTLNYRSSFYLNMYDSTWNENLYYSEKLDALIVFTVENQKLK
                     LFGVFAPVIPILDELCEEISGEFTEIEFYFYPDQLGIDDVQYTELQTSKYLMVRSNKEL
                     DFKGYKFPVLTEF"
     misc_feature    complement(9922..9938)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.8"
                     /tool="antismash"
ORIGIN
        1 tggtattttg aaaaacaagt agagcgctgg aaaatagcaa tacagaagtc tcagcaatat
       61 gaattagtaa tactcgatgg ggatatatat cagccactta gttataattg gtgttttcgt
      121 tttgatatat ttaatcagcc gttatcttta atagaaaact tttataaaga aaagatgata
      181 aatagagaaa ttggttttcc agatcaatat ttttatttat atacaagtga tgaagagctt
      241 agaaaacgga aagagtttga tgggacgaaa aaaagaagaa actttgaaaa acatttacat
      301 atatctaaag catttcagcg ttattatgaa aatctcaata ccgtcacaga cgggtactgt
      361 aaattgattg aagcaaaaag tataaagtcg aatgaattag aaattgtgaa aagtttaaac
      421 agcttaaaag tatgtgaaga aagacgtttt gatgtttcga ggttagatgc tattacggag
      481 tggttaaaag aaaatcgtgc ataaagtaaa aacaacgagt gttttagaag gcactcgttg
      541 tttttttgtt tccatataaa tataaaggaa aattttgtag aatatcttta ttttttaaca
      601 gaataatctt tcaatttcac gaaaaaagaa atacaatata aggaaatata tataaaaaat
      661 atgatagggg atgggatgaa ctattttgaa tgaaggaact gttttatata agaaataatg
      721 aggtagtaag aacagagata tttgggagga aacaagcaag atgaaaacat tatttaaaat
      781 cataggaatt atagctggta tggcggtaat cggggtaggt gtaacgtatg gtatgctgta
      841 ttatctgaat aatagtaaac caactgcgaa aaaggcatca ccagccgctc cggcagtaga
      901 agtgctagct gatagtaatg taaaagccga agatgcaaag atattagaaa atggaaatta
      961 ttcattgccg aatagtggtt ttaataaaaa cttcaaatgg acagatgaga acatacagac
     1021 agcattacat gaaatggcac atcaaaaagc gaaagctgat caaaaatggg gctatatttt
     1081 tattacacaa gaacgtattg aaagtttact tgaaattata aatagtaatg atgttgcaca
     1141 aaaaagtaca tatgtggata ttttagaacg atggaaacaa ggaaaatatg aaaaggttga
     1201 tgtggaccac aacaagattt ggaaattaca aagtgggaat ttaggagaag ggaaaggagt
     1261 aatgtcagaa aaggagcaaa aagaattaat taatcaagtt tttatacaaa aaggtacata
     1321 ttcaggtagt aaattaattg caggtgaggt acaagctaat aaataatata catattgatt
     1381 ttgaaacacc tgtattgtta tgaacacaaa tgttttaaca aaaatgagta attgcttttt
     1441 agagcagtta ctcatttttg taattactcg cttacttttc tattctctct aataatgtaa
     1501 gaaacgattg caaaactggg gatatccatc tagatttatg atagattacg tggctttgaa
     1561 tgacggctgg agtttcgatg ggctgaaaac agagttgttc tttttgatta ctttcttgta
     1621 caacgatgta agggagaata gaaattccta actcacacat aacggattgt ttaatgactt
     1681 caacattgga agtctcaaat gttttattag gatttcttcc gcttttacgt aaaaaacgat
     1741 ctaccattgg tctatagcca cagctttgtt ctgtaaatat aaattgagta tcttgtaata
     1801 aaaggagtgg atcagtgttt gaagggaaat gagcagggaa aatccatcca aatgtttcat
     1861 tacataaatt gcacgtaata aaatcattat gctctatact ttctccaatt atgaagataa
     1921 catcggtttc tccttcatgt aattttttca atgcttgctc attggtattt gtctcaagta
     1981 cgatatttac tttcggattt tcctgtttgt aagtacgtaa taattgtggt aaccgataca
     2041 ctgccaatga ttcattagat gtaatagcca atgttccttc taaatggtca ctgttttgag
     2101 ggatttcttg cgctttatca taaatagtaa gtaattctaa tgcatacgga tgaaattgat
     2161 gacctgcttt tgtaagaagt attttttttc ctaatcgatc gaatagggga atgtttaaat
     2221 cgttctctaa tgctttaata tgggctgtca cagtagattg cgcataacct aatgcatgag
     2281 cagctttaga aaagctacca tatttaacaa tggcacaaaa tgttttaaca tgtctcattt
     2341 ccataaatga tacctctttt attatcatga tttgtgaatg attatatcat gtatttcaat
     2401 ttttcaaatc attaaatttc ttttaaaatt aagttgtgga gaaattcatt caattgaaat
     2461 tggaggagaa aaaatgagtg aaaagagcta tcatgtagct gtagttggag ctacaggcgc
     2521 agtagggcaa aaaatcattg cattgttaga gagagtaacg aaatttaata tagaagaaat
     2581 tacattactc tcgtcaaaac gatctgctgg taagatagta caatttaaag gacgagagat
     2641 taaaatacaa gaggcaaaat caactagttt tgaaggtgta gacattgtct tttttagcgc
     2701 tgggggagaa gtttctagac aatatgtaaa tcatgcagtt tctagtggtg caatcgtaat
     2761 tgacaacaca agtgaatacc gaatggcaca cgatgtacca ctcgtcgttc cagaagtaaa
     2821 tgcacatact ttaagggaac ataaaaggat cattgcagtt ccgaattgct cagcactaca
     2881 gatggtaaca gctcttcagc caattcgaaa atcatttggt ttagaacgaa ttattgtttc
     2941 aacgtatcaa gccgtatcag gctcaggaat tcatgcgatt catgaattaa aagaacaagc
     3001 taagtcaatg cttacaggtg aagaagtgaa gagcactata ttaccagtga aaaaagataa
     3061 aaaacattat ccaattgcat ttaatgtatt gccgaaagtg gatatattta cagacaatga
     3121 tttcacattc gaagaagtaa agatgattca agagacgaag aaaattttag aagatccaaa
     3181 tctgaaaatg gcagctactt gcgtcagagt tccagttgta tcggggcatt ctgaatcagt
     3241 ttatattgaa cttgaaaaag aagcgaccgt tgcagaaatt aaagaagtac tactgaatgc
     3301 accaggtgtt attttacaag ataatcctag tgaacaaatt tatccaacgc cattatatgc
     3361 agagggtaaa agagatacat ttgtaggtag aatacgtaaa gatcctgata catcaaaagg
     3421 attccatctt tggatcgttt ctgataattt attaaaaggt gctgcttgga actcagttca
     3481 aatcgcagag acattggtgg aaaatggggt tatttagtat ggatttatat aaatattgaa
     3541 aatagaagga tatttgggaa accgttaatt ataatggttt cctgtttttt acttaatgag
     3601 tattttcctc ttacttcaat taaaatttaa tttaagttag tttttatagt gtttttataa
     3661 atatcaactt tatctgcaga gaattttaaa aatctaggct ctccgttaat tgtgagatta
     3721 atattaagtg ataaaggcat ttcaaccttt ttcactacat cttctatagc aatatactca
     3781 tcttcttttt cgttataaaa acaatggaac taattaatac ctcattattt tcaatatgaa
     3841 tactacacat catagggaat ttctcatcat tatgtattat taattcgcca agtactattg
     3901 aaccgtttaa ttcttctgta aaatttattg tttccactgg ttttacccac gtttcatcgc
     3961 actcttcatt ttccgcttca tctattgccc attcccatat tgggttttgt ttcaggtcct
     4021 caatagttaa ttcatcaatt ggtttgctat tacacattaa tatatactcc ttttgccatg
     4081 aaaatggatg agtaaaaaat ttattcatta attgtaaaat gtggctattt gcaatcctat
     4141 aaatacaagt ttgctcgttt caataaatag ttgatcgcaa ataagaagtt tcgatgtaga
     4201 gtattatcca tttgatatta ttaaatctag ttttgtaatg ttgaacttat ttttacaacc
     4261 tattaaaagt ctattcttac ggtaatctta acaaggtttt aactagacag taaataatgc
     4321 cttttgaaaa ggaaaaccgt ggttgtgatg ggggaaatag caaaaaacat cgctgatttg
     4381 ccattcataa aaatagactt ttcttagcag aaatcactaa ttataaatac ttataaaatt
     4441 agctctgata tagcagatat tgaagcgaag aaaaataaag atgggagtgc atattggaaa
     4501 tacgaagatg accaaacaaa attatggaat atacgactat agaactagaa aatgatgaca
     4561 taaatctaaa tattattgtt taaattcata ttttataatg tccatttagc ccgcattaac
     4621 tgcccgtaaa ccccctctga ttaaagtttc actttataaa aatgagccca tagtaaaaat
     4681 aacctaaaaa taggcatatg aacaaggtgc atcatctagt gataggtcat attttgttgg
     4741 tgtacgtatt gaacttagtt tatcccgcta tttgtgggca gtaaaactcc cacctcaaaa
     4801 ttcaactgga gcaaagaagt taggtgggag ccctgctgcc cgtaaacgcc cgattggtgc
     4861 gggctaataa tcagtggggg atgaacaaaa cccctactga ttaaagtttc actttatagc
     4921 gctatttcta cttagattta acgaacagaa atataggaaa gtatcgatgg ggttttagtt
     4981 tataaacgga ggtcatgaag atgaataatt ttcaacaaga actacaagcg ttaagcctta
     5041 atgattatca gcctggaaat attgtgtatt gggaccagca aaaccaatat ccatattact
     5101 atattacaga tgatgctcgt cgctgtggcg gctgtggcgg tggtcgttgt ggtggttgtg
     5161 gtcgttgtgg cggtggtcgt tgtggtggat tccgttgttt tggttgcttc ggttgcttta
     5221 gttgttttgg ttgtggtggt tgctctaatt gctctaattg ctctaattgc tctaattgtt
     5281 ttgatggttt taatggtact actgtaacat ttgaatattg ataagagaca aaaaggaatc
     5341 cattttgaaa aaagattgat cgaaacggat tcctactttg tgaagcatta tataattttt
     5401 ataaaaatgt gctaacaagt ttgcaaacgg acttgctaac acctatacga aatagacata
     5461 aaatatcgct gatgtgtagc ttaataatac gataataatt tagcacttat aatgaatcat
     5521 aggttattag aaaagaaacc ataaaatgta atgttttata tcattataaa aagagattat
     5581 tctaataggt cgttgtgtaa cggctgcata ggataatctc tttttatgct tatttttgta
     5641 aagaatgtac tgttattttg agttgctcaa taaatttccg ggtagcagcg cacatatatt
     5701 tatctcttct ataaataagt cctatatcta ttgttggagt ggggttttca attttaatag
     5761 cttgtatgtg ctcattttgc aaaaagtcta tatacggttt tggtaacacg gtaatcccca
     5821 taccttgtgc gaccatttga attaaagatt ccattgtact aatttctaaa attggctttg
     5881 gtttaaagtt aaagttttgg caatgagatg taattagttc tgttaaatag aaatttttag
     5941 gtagaagaat taaaggatag tcctgtaatt cttcaataga tacatactta cgttcagtta
     6001 aagaatggct ggttggcaca actaatgtaa gttcactttt atatagtggg atagaaacaa
     6061 tctctttatc ttgcaccggg agaaatgtaa taccaatatc taattcattt tgtaataatt
     6121 tttcacgaat ttctcctgta cgaagcccca acacagagag ttcgatattt ggatattgct
     6181 tattgaaatc taatatagct ggtggtagta aataatttac tacggttaac aaagatccta
     6241 ttgttaagga accatgatgt aatccattta aatcttgaat ggcagaacga gcttgttcga
     6301 cttcgtgaaa gattgtttta ctgtgcgata ataaaactct tcctgcttca gttaaagata
     6361 tttttttacc aattcgatcg aaaactggca tgcctacttc atgttctaat gcacgtattt
     6421 gttggcttag tgaaggttgt gaaatattta atttctcggc tgcttttgtg aaatgtaatt
     6481 ctttcgaaac agctaaaaag tattctaatt gtcgtaattc gattgaaatc acctcaagtt
     6541 taatcatagg atatacctat cattattata taaataattg aattgaacaa tggttttgtg
     6601 ggatatataa tcactttatc agaaatggat tgattaaaaa attagatgag gtgaagaaga
     6661 attgttgaaa tctagtgtat taccgggcag ttattcacaa gtacttcaaa aaaggtatga
     6721 ggaggccgta ccgacaggaa tatatcattt aacgccttta tatgtaaaag aagcaagggg
     6781 agcgataata acagatgtag atggaaatca gtttattgat tttgctggtg gaattggtat
     6841 gcaaaatgtt ggacattgtc atccgaaagt tgtaagagcg gtacaaaaac aagcagaatc
     6901 atttacacat ccttgttttc atgtaactcc atatgaaaat tatattactt tagcggagaa
     6961 aattaatgag aaagtgccag gtacaagtaa gaaaaaaacg atgtttgcca atagtggtgc
     7021 ggaagcgatt gaaaatgctg taaagatcgc acgtaaagta acagggagga gtaaagtaat
     7081 ttcgtttgaa cgaggatttc atgggagaac attaatgaca atgtctctat cgaccaaaga
     7141 aactccatat aaagcaggat ttggtccgtt cgtttcagac atgcatatat tgccgtatcc
     7201 gtactattac agagctgaag aaggagtttc tcctgaagaa gtggattctc aagtcatcta
     7261 tcagtttgaa aagtttttag aagaagtatc acctgaaaaa gtagcagcga tcgtaatgga
     7321 accagtacaa ggagagggtg ggtttattgt tccttccatt tcatttgtaa aacgattacg
     7381 agaaatttgt aatcaatacg gtattttaat gataatggat gaagtgcaaa ctggatttgg
     7441 ccgaacaggg aaatggtttg caacggaaca ttttcatata gaacctgaca taatgacttt
     7501 gtcaaaatca attgctggcg gtttaccatt aagtgctatt gtcggtcgtg cggagttgat
     7561 ggacattcct gggagaggac aaattggtgg aacttttgcc gggagtccgt tatcatgtgc
     7621 agccggtctt gctgtatttg aaataatgga agaagaaaat ctagttgaaa gggctcagca
     7681 aattggaaat agaataatgt cacatctaca tcaaatgcaa ttaaagtacg aaatgattgg
     7741 tgatgttcgt ggaatcggtg cgatggttgc aatggaactt gtaacgaatc gcataacgaa
     7801 agaacctgca aaagaactaa cagtaaagct aattgaagat ttttggaaga atgggcttat
     7861 aagtattgga gcggggatat ttgataatgt actacgcttt ttaccacctc tagttatctc
     7921 aaacgaagaa atagatagag gatttgaaat aataaatgag tcttttgaaa agttgtgccg
     7981 caagtaagag gagggatttg aatgactaaa gtatatgaaa tcctatcttt aaatctagga
     8041 ttgccaaaag aggttatgta tggtgggaag ttaattcaaa cagggataaa taaaaagcaa
     8101 gtaaaggaac ctgttttttt atcattttta aagtttaatg gagatggaca ggcggattta
     8161 gttcatcatg gaggggtaga taaagcggtt tgtgtttata ctggtgacca ctatccttac
     8221 tgggaaaaag agttgaatca ggaacttgta tatggggctt tcggtgaaaa tataacagta
     8281 agcggaatgc gtgaagaaga tgtttgtatt ggtgacacgt ttcaactcgg agaagcgatt
     8341 gtacaagtaa cgcagccaag acaaccttgt tttaaattag ccaagaaata taatattcca
     8401 aagttgccat tatattttca agatacagga tacacaggtt tttattttcg agtattaaaa
     8461 gaagggtgga tattaccggt agatacttta aaattgttga agcttgatcc aaaaggtgtg
     8521 tcaattttgt ttgcgaatcg cataatgcat aaagaaaaac aaaactctga aggattaaaa
     8581 aggattttag aagtaaatgc actgtctact agttggagaa agacgtttga aaagcgagtg
     8641 aagggagaag aaattagtac gaaggagcga cttgagggaa ccaaagaata ggtgaattat
     8701 atttgcataa aatggtgtat ttctattatc ggaaagataa gtaggaagca ccatttgtta
     8761 tataataaac atgatattat tcaaaaatct taatactagt ttattcgact tgttttttaa
     8821 taatcaaaac ataaaataaa ttaaaccatt tttaaaagtt ttatgatact atattgattt
     8881 gaaaggccta ttttttgaga gtaggtcata taagtatatc taaattaatg gaatcgacgt
     8941 atggagggac aacatgggca atgttgaaaa aatggcaaac tttctattag aagaaccgag
     9001 aagagaacag ttatttcctt tatttgaaga agtgtttggg attacgagcc aaacattgca
     9061 ggatttttca gagaaagggt attgggatta tacatataaa gcattatcat ttttacaaga
     9121 tgataatgcg attgcaaatg ttgcggcatt ttcacttccg cttttaatta atggtgaaaa
     9181 aataaatgca acgggtattc aatcagtaat gacacatccg aattatcgta gacgaggatt
     9241 aatgaagcaa ttgtttagta agatgataga ggaaattgat gagcaatgcg agtgtgcggt
     9301 cttgtttact gaaaatccgg aactatataa accatttggt tttcaaattg tgcaagaata
     9361 tttgatgaca gtatcatacg aaaataaagg ctacaaagat tctcaactta aaaagttaga
     9421 tttttataat gaagaggata gacaattaat acatgagatt attgaaaatg gccaaaggct
     9481 ttctaataaa ttttcaacgt taaattaccg ctcttcgttt tatttaaata tgtatgattc
     9541 aacatggaat gaaaaccttt attattcaga gaaattagat gcgctgattg tttttacagt
     9601 agaaaatcaa aaactaaaat tatttggagt atttgcgccg gtaattccga ttttagatga
     9661 attatgtgag gaaatctctg gggaatttac ggagattgaa ttttatttct atccagatca
     9721 attaggaatt gatgatgtac aatatacaga attacagact agtaagtatt taatggttcg
     9781 aagtaataaa gaattagatt ttaaaggtta taaattcccg gtattaactg agttttaaat
     9841 attaatttaa atgctttttt atggaggaat tatgaaaaca tcatatatac caaatcatat
     9901 tgagcaactt actgtaatga acgcacctaa attttacaaa cttgaagaca atgatacatt
     9961 cattgtccat tcgaaagaag aaacagattt ttggctgaaa acccattatg gttttgaagt
    10021 aatgaatggc catgtatttt atgatgagat aatgacagat tttcaggctg aggttcaatt
    10081 gcgcatgaat ccaaatgcga catacgatca agcgggttta tttgttatga tttctgagaa
    10141 gtgttggtta aaaacatctt tagaatatat tcctgagggc ccatcacatt taggtgctgt
    10201 cgtaacgaat aatggctatt ctgattggtc aacgcaagac tatccaaatg agtttgctaa
    10261 gcaacaatta cgttttcgaa ttattcgaaa acttggcgat tatacaatat atgtgaaaac
    10321 g
//
