LOCUS       NZ_CP059997            22026 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Amycolatopsis sp. CA-230715 chromosome, complete genome.
ACCESSION   NZ_CP059997
VERSION     NZ_CP059997.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. CA-230715
  ORGANISM  Amycolatopsis sp. CA-230715
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 10363158)
  AUTHORS   Jorgensen,T.S., Gren,T., Oves-Costales,D., Ortiz-Lopez,F.J.,
            Carretero-Molina,D., Gonzalez,I., Blin,K., Genilloud,O. and Weber,T.
  TITLE     Complete Genome Sequence of Amycolatopsis sp. CA-230715, Encoding a
            35-Module Type I Polyketide Synthase
  JOURNAL   Microbiol Resour Announc 10 (38), e0080521 (2021)
   PUBMED   34553997
REFERENCE   2  (bases 1 to 10363158)
  AUTHORS   Joergensen,T.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2020) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2019
            Assembly Method :: Flye v. 2.4.1-geb89c9e; Unicycler v. 0.4.8-beta;
            SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology :: Illumina MiSeq; PacBio RSII; Oxford
            Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 09:21:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,592
            CDSs (total)                      :: 9,498
            Genes (coding)                    :: 9,434
            CDSs (with protein)               :: 9,434
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 10 of 64
            Pseudo Genes (incomplete)         :: 60 of 64
            Pseudo Genes (internal stop)      :: 2 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:58:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6085298
            Orig. end                         :: 6107324
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059997.1.
            Annotated using prokka_raw 1.14.6 from
            https://github.com/tseemann/prokka.
            Bacteria and source DNA available from Fundacion MEDINA, Grenada,
            Spain.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22026
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="26"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22026
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22026
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12026](-)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12026)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     gene            84..1139
                     /locus_tag="HUW46_RS29250"
                     /old_locus_tag="HUW46_05802"
     CDS             84..1139
                     /EC_number="1.1.1.93"
                     /GO_function="GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA]"
                     /GO_process="GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239495.1"
                     /locus_tag="HUW46_RS29250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05802"
                     /product="tartrate dehydrogenase"
                     /protein_id="WP_215541999.1"
                     /transl_table=11
                     /translation="MTDYRIASIPGDGIGVDVTVEARKVLDAAAAKHRFGLKWTEFDWS
                     CERYAQTGAMMPEDGVEQLSAFDGIFLGAVGFPGVPDHVSLWGLLIPVRRAFAQYVNLR
                     PVRLLPGTTSVLAGRSADELEMVIVRENSEGEYSTVGGRHNKGQQGEFALQESVFTRVG
                     VERIIRYAFELAKTRTGRVCSATKSNGIIHSMPFWDEIFAEIAAEYPDVHSEQCHIDAL
                     AARMVQAPDRLDVVVGSNLFGDILSDLAAAVTGGLGMAPSGNINPPGEYPSMFEAVHGS
                     APDIAGQGIANPVAQILAGAMMLDHLGEKAAANDVNTAVEKVLSEGTVATPDLGGKSTT
                     TELGSAIAEAL"
     gene            complement(1402..2484)
                     /locus_tag="HUW46_RS29255"
                     /old_locus_tag="HUW46_05803"
     CDS             complement(1402..2484)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1229: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /locus_tag="HUW46_RS29255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05803"
                     /product="IS630 family transposase"
                     /protein_id="WP_215542000.1"
                     /transl_table=11
                     /translation="MDERAELRALLNSADVPATVATRARIVLWRAESRLKQDVAVLAGV
                     SRPTVDLWLGRYGAEGIAGLLDRPRGAPREQVPAAIRARILAVSRMSPPVETGLSHWSS
                     REMAAFIVRTEGRYVSHHYVAKLWRENGLRPQKQGTFKVSGDPEFAEKVADIVGLYLHP
                     PGAAVVLSIDEKTQIQALDRTQPLLPIDFDASEQRTHDYVRHGTTNLFAALNVGTGEVV
                     GECAPVRDGVRFLAFVKKAVKPHADKEVHVVLDNLSTHTTPEVQAWLADNPNVRFHFTP
                     KGSSWINQIETWFGIMTRQSIRRGTFTSVKVLIKQIRDYITHWNTTATPFTWTATADEI
                     LAKVRLVQTNIKKLVDNNAK"
     gene            2698..3192
                     /locus_tag="HUW46_RS29260"
                     /old_locus_tag="HUW46_05804"
     CDS             2698..3192
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003079606.1"
                     /locus_tag="HUW46_RS29260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05804"
                     /product="DUF4262 domain-containing protein"
                     /protein_id="WP_215542001.1"
                     /transl_table=11
                     /translation="MCWQCDNPGKSVGDYLDYIAGEITKYGWIVQSVEEDAVRASWAYT
                     VGLTRTGRPELVVTGLPGEVSAGLLNEFASHLGHSDDTPVPGERITFENGGPSIELVEL
                     AEPSVHLLMAVGLYGKKIRGLQLVYADDEGRMPWERAYNDGQGGQPVLGSRATAPRGDD
                     P"
     gene            complement(<3325..4011)
                     /locus_tag="HUW46_RS29265"
                     /old_locus_tag="HUW46_05805"
                     /pseudo=""
     CDS             complement(<3325..4011)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641050.1"
                     /locus_tag="HUW46_RS29265"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05805"
                     /product="GntR family transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTVPLADIEPVSRESTAGIIARQLRDAIMNGSLQPGTQLGETELA
                     AQFQVSRGPLREAMQRLVSEGLLRSERHRGLFVIDLGPEDVYDIYTARSAIERAAALRI
                     VRGDREAVAAVLAESVHEMATAAEQDDPTALSEADLRFHEALIAASGSKRLVRMARTLL
                     IETRMCLSALQNTYQGIEERVTEHNQIIEAIRAGDEDAVLSLLEAHMEDAVQRLAPGNS
                     LQSGQAP"
     gene            complement(4008..4727)
                     /locus_tag="HUW46_RS29270"
                     /old_locus_tag="HUW46_05806"
     CDS             complement(4008..4727)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665077.1"
                     /locus_tag="HUW46_RS29270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05806"
                     /product="maleate cis-trans isomerase"
                     /protein_id="WP_215542003.1"
                     /transl_table=11
                     /translation="MPVIGFIYPDHAAEDDYPLAEGVLGAGVRLPVEHIYGTDLHAVPE
                     LLDLGSPARLAEGARLLAKHEPDAVVWACTSGSFVYRPDGAREQVEKLAEAAGKPASST
                     SFAFVNAAAALGVRKVAVAASYPDDVAKLFVEFLASGDVEVVSMSSEDIDTAAEVGRLS
                     PEAVVELAVSHDHPDAEAVLVPDTAMRTLGVLGAIEERLGKPVLTANQVTIWEGLRLAG
                     ETKTVDSFGTLFRRGGE"
     misc_feature    4752..4768
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.95"
                     /tool="antismash"
     misc_feature    complement(4753..4771)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.25"
                     /tool="antismash"
     gene            complement(4800..5540)
                     /locus_tag="HUW46_RS29275"
                     /old_locus_tag="HUW46_05807"
     CDS             complement(4800..5540)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465986.1"
                     /locus_tag="HUW46_RS29275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05807"
                     /product="Asp/Glu/hydantoin racemase"
                     /protein_id="WP_215542004.1"
                     /transl_table=11
                     /translation="MDFSLPEFEGPLAQRGIGVIAPFDLALERELWRWVPMEVSLHLAR
                     TPYEPVPVSMEMAQLVSNSAHLATATRDVLHVEPEVVAYLCTSGSFVNGVDYERSLTKA
                     ICDAGAPDAVTTSGALAEVLQQLGSRRIAVITPYDADLTGSLHRFLAELGVETVASEHL
                     GLGGGIWKVSYRTIAERILKADHPDAEAIFVSCTNLPTYDLIEPLETALGKPVLTANQL
                     TMWACLNRMRLPIVGPGNWLREVS"
     gene            5635..6612
                     /locus_tag="HUW46_RS29280"
                     /old_locus_tag="HUW46_05808"
     CDS             5635..6612
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465985.1"
                     /locus_tag="HUW46_RS29280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05808"
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="WP_254124994.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 1.1e-49, bitscore:
                     167.3, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIGTDSPVLAVLCGDKHPPDMHSIEQAAEVRYTGADGLAGALAGA
                     DALFVYDFLSTAVPGAWHAADRLRWLHIASAGVDPVLFPGLVDSEVVLTNSRGVFDDSI
                     AEYVLGVILTFAKGIARSLDLQRENRWLHRESERIGGKRAIVVGTGPIGRSIARLLRAA
                     GMRVSGAGRRARDGDPDFGTVHSSEALAEQLPEADYVVAVAPLTERTKGMFDAKAFAAM
                     KPSARFVNVGRGELVVTGDLVAALRDGQLAGAALDVFETEPLPAESPLWTMPEVLVSPH
                     MSGDFVGWRNTLVEVFADNFQRWREGVPLRNVVDKKLGYVPSSP"
     gene            6663..8075
                     /locus_tag="HUW46_RS29285"
                     /old_locus_tag="HUW46_05809"
     CDS             6663..8075
                     /EC_number="3.5.1.4"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1105: amidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155859.1"
                     /locus_tag="HUW46_RS29285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05809"
                     /product="amidase"
                     /protein_id="WP_215542005.1"
                     /transl_table=11
                     /translation="MPANTGTLLTASELVAAYSTGELSPVEATQAALKAIEDRDGDLNA
                     YCLVDAEAALEQAKGSEERWRQGNPIGWLDGVPTSIKDMFLTQGWPTLRGSKAIDPDQP
                     WEVDSPVTARLRENGLVLLGKTTTPELGWKGVTDNPLTGITRNPWDTTRTAGGSSGGSA
                     AAVAAGMGELSVGTDGGGSVRIPASFCGIVGLKPTHGRIPLYPASPFGALAHAGPMARS
                     VDDIALLLDVLALADFRDPAALPPLAGDYRSAVRRDVRGINAAFSPDLGYVKVDPEVAK
                     IVTSAVAALTEAGLNVTETAPGFSDPMDAFDVLWSTGAAKWLDSFPEGSAEVIDPGLRK
                     VWEKGLTYSASDYLGANAERAALGIHMGEFHTRYDVLITPTMPIVPFEAGVDVPPGSGL
                     SSWPEWTRFSYPFNMTQQPAISVPAGFTEGGLPVGLQIVGPRHSDDLVLAVAKLLEEIR
                     PWPVDRPAGVLR"
     gene            8124..9098
                     /locus_tag="HUW46_RS29290"
                     /old_locus_tag="HUW46_05810"
     CDS             8124..9098
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499287.1"
                     /locus_tag="HUW46_RS29290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05810"
                     /product="aldo/keto reductase"
                     /protein_id="WP_215542006.1"
                     /transl_table=11
                     /translation="MRRTILGKSGLDVSRIAFGTWQLGGDWGSFDEQTAVSAIQHAREL
                     GVNFFDTAQAYGFGKSEALLGKALRDELKRDRDSLVIATKGGINPGGERPRDAGRAWIR
                     KGVEESLEYLGLDHIDLYQVHWPDADTPAEETAGVLQELVDAGKIRHAGVSNYDAAQLA
                     DFDRTRPVETLQPPYHLFRRHIETDPLPYAREHEIGVLAYSPLGSGLLTGSLTADATFE
                     ASDWRSKATAFGGDSLRRNVEAVAELAEFAKTKDATVSQVAIAWVLAQPGVHVAIVGAR
                     KPKNIEDSLAAADLDLSADDLAEIERITAKGVQVDGASPEGVA"
     gene            complement(9114..10004)
                     /gene="pqqB"
                     /locus_tag="HUW46_RS29295"
                     /old_locus_tag="HUW46_05811"
     CDS             complement(9114..10004)
                     /codon_start=1
                     /gene="pqqB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229609.1"
                     /locus_tag="HUW46_RS29295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05811"
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="WP_215542007.1"
                     /transl_table=11
                     /translation="MKVALLGTAAGGGFPQWNCACALCRSGAAPRTQDCLAVSADGKGW
                     YLVNASPDIRAQVLATPELAPGPGPRETPLRGAFLTDGELDHTLGLLMLREGAGMTVWA
                     PDAVLHATRHALPMQAVVAGYGEWTWRPLSSGAVIDGLRVSVLPVGTKRPKYATSSTVD
                     GPWVVAYRFDDPVTGGSLVYAPCLASWPPGFDHFVRGASFVLLDGTFHGGGEMSGATGS
                     SADGAQRAMGHLPVSGADGSLALIRRQLVLAPDQRWAYTHLNNTNPLVDHDSAAHQEVL
                     AAGVEIPHDGTVLTL"
     gene            complement(10001..11080)
                     /gene="pqqE"
                     /locus_tag="HUW46_RS29300"
                     /old_locus_tag="HUW46_05812"
     CDS             complement(10001..11080)
                     /GO_process="GO:0018149 - peptide cross-linking [Evidence
                     IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079180.1"
                     /locus_tag="HUW46_RS29300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05812"
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="WP_215542008.1"
                     /sec_met_domain="PTHR43273 (E-value: 1.5e-30, bitscore:
                     105.5, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 2.5e-20, bitscore: 72.6,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 1.6e-10, bitscore: 40.4,
                     seeds: 166, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 2.9e-11, bitscore:
                     42.7, seeds: 281, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVIEPPLGLLAELTHRCPLHCPYCSNPLELLTRDAELGTEDWLS
                     ILTQARELGVLQVHMSGGEPLARPDLPRLVEHATGLGCYVNLVTSGLGLTDTRLADLAD
                     RGIAHIQLSVQGADAERADRLAGAKAFHHKIRAAELVKAHDLPLSVNVVLHRQNHDQLA
                     GIIALAEEMGADRLELANTQYYGWALRNRDALMPTPDQLAAAEPVVRAAAERLRGRMEI
                     IYVVADYYEPYPKPCMYGWGARQLTVAPNGDVLPCPAATAITTLELDNVTKRPLAEIWY
                     DSTSFNAYRGEDWMREPCRTCDRRGLDHGGCRCQAFLLTGDAAATDPVCSRSPDRGVVD
                     LVLAAPRHEPPLVMREVTR"
     gene            complement(11071..11325)
                     /gene="pqqD"
                     /locus_tag="HUW46_RS29305"
                     /old_locus_tag="HUW46_05813"
     CDS             complement(11071..11325)
                     /GO_function="GO:0048038 - quinone binding [Evidence IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PqqD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005456330.1"
                     /locus_tag="HUW46_RS29305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05813"
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="WP_215542009.1"
                     /sec_met_domain="PqqD_RRE (E-value: 7.2e-22, bitscore:
                     76.0, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 7e-10, bitscore: 38.2,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKPKLRTGVRLTYDRTRETHVLLFPEGVLVPNATAAAVLGLCDG
                     EATVEEITASLGERYSGVRTQEVVALLEKLADRQVVRWT"
     aSDomain        complement(11080..11316)
                     /aSDomain="PqqD_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing protein in a pyrroloquinoline
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_HUW46_RS29305_PqqD_RRE.1"
                     /evalue="2.10E-24"
                     /identifier="RREFam006.1"
                     /label="HUW46_RS29305"
                     /locus_tag="HUW46_RS29305"
                     /protein_end="82"
                     /protein_start="3"
                     /score="76.0"
                     /tool="antismash"
                     /translation="PKLRTGVRLTYDRTRETHVLLFPEGVLVPNATAAAVLGLCDGEAT
                     VEEITASLGERYSGVRTQEVVALLEKLADRQVVR"
     gene            complement(11328..12026)
                     /gene="pqqC"
                     /locus_tag="HUW46_RS29310"
                     /old_locus_tag="HUW46_05814"
     CDS             complement(11328..12026)
                     /EC_number="1.3.3.11"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR02111"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150533.1"
                     /locus_tag="HUW46_RS29310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05814"
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="WP_215542010.1"
                     /sec_met_domain="TIGR02111 (E-value: 3e-80, bitscore:
                     268.1, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTSAPLSETEFVAALRGLSHRYWGTHPFHHRLHDGDLDERELKI
                     WAANRWYYQCVLPRKDAAIISNCPVPEIRRHWLDRIVYHDGAREGEGGIERWLRLCQAV
                     GLTREEVLDERHLAPGVRFAVDAYVTFARTRPWVEAVASGLTEMFAGHLMRRRVSDMLA
                     NYAWIRAEDLDYFTNRIDAVSGEGQATVDIVVRYSTTRTEQEAAIAALSFKCDVLWSIL
                     DAVERACEKD"
     gene            complement(12093..12191)
                     /gene="pqqA"
                     /locus_tag="HUW46_RS29315"
                     /old_locus_tag="HUW46_05815"
     CDS             complement(12093..12191)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033797.1"
                     /locus_tag="HUW46_RS29315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05815"
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="WP_215542011.1"
                     /transl_table=11
                     /translation="MKETQVEVWTTPDFVEYETPMEVTAYAARMED"
     misc_feature    complement(12159..12174)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 23.05"
                     /tool="antismash"
     misc_feature    12328..12343
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.94"
                     /tool="antismash"
     misc_feature    complement(12347..12360)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.72"
                     /tool="antismash"
     misc_feature    12348..12361
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.62"
                     /tool="antismash"
     misc_feature    complement(12350..12363)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.37"
                     /tool="antismash"
     misc_feature    12351..12364
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.37"
                     /tool="antismash"
     gene            12514..13497
                     /locus_tag="HUW46_RS29320"
                     /old_locus_tag="HUW46_05816"
     CDS             12514..13497
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631253.1"
                     /locus_tag="HUW46_RS29320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05816"
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="WP_215542012.1"
                     /transl_table=11
                     /translation="MSLRRSAVFAAAFAFAALTVGGPAVTSAAPAAAPKKVASGLNEAW
                     SVDFLPDGTALFTEKDSKKIRKIDKSGKVSDVQTVPGVSVTKEAGLLGIAVSPHYAQDQ
                     TVYVYYTTSSDNRIAKLKLGGDPVPILTGIPRGTQFHQGGRIKFGPDGFLYAGTGDAKK
                     SSNAPDKNSLGGKILRITTDGKPAPGNPFGNPVYSYGHRNVQGLTWVGDQLYASEVGES
                     TYDELNKIEAGKNYGWPKCEGKCSEPGMTNPVNQWSTSDATPSGIANYKGKLYMAALKG
                     GTYKLDTAGKGGKLYTDLGRTRDEVAGPDGRLWIVTPGAIYTADGN"
     gene            complement(13565..14929)
                     /locus_tag="HUW46_RS29325"
                     /old_locus_tag="HUW46_05817"
     CDS             complement(13565..14929)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641036.1"
                     /locus_tag="HUW46_RS29325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05817"
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="WP_215542013.1"
                     /transl_table=11
                     /translation="MPLMPVTDSMFLLVETREHPMHVGGLELFEKPEGAGPEYLSELRQ
                     RLREDTNMRPIFRRRPANPVNTAGHLAWSEDNDLDIDYHFRHSALPQPGRIRELLELTS
                     RWHGTLLDRHRPLWEIHLIEGLQDDRFAIYSKIHHALLDGVSALRHLQGTLSDDPGDLD
                     CPPPWGSREVNGHRNGRAPTSLFKTAGKTLSQLAGIAPAAYKVANEAFREHTLTLPLQA
                     PKTMLNVPIGGARRFAAQSWSLERVRAIATAAGVSRNDVVLAMCSGALRDYLIEQRALP
                     DAPMTAMVPVSLRAKTDDSEAAGNNIGALLCNLGTDKADPADRLAVIHKSMRDGKRMFK
                     QLTPLQVLLLSGINVAALGASPIPGVVNNTKPPFNLVISNVPGPRSQMYWNGARLDGIY
                     PASVLLDGQALNITLTSNGDNLDFGITGCRRSVPHLQRILTHLDTALAELEFATR"
     misc_feature    complement(15002..15016)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.79"
                     /tool="antismash"
     gene            15052..16050
                     /locus_tag="HUW46_RS29330"
                     /old_locus_tag="HUW46_05818"
     CDS             15052..16050
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GST_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814099.1"
                     /locus_tag="HUW46_RS29330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05818"
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="WP_215542014.1"
                     /sec_met_domain="GST_C (E-value: 1.3e-06, bitscore: 27.7,
                     seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIPTDPETGEFHRSANHFDARITADGRDGWPVEPGRYRLVVSRA
                     CPWASRGLIVRRLLGLEQALSVAVADPIQDEKSWRFTLDPGDRDPVLGIKYLGEAYFAA
                     EPGYDGGISVPAIVDVPSGKLVTNDYPRITLDLSTEWTAHQREGAPELYPERLRDEIDE
                     VNRGVHADVNAAVYEAGFASKQAAYDDAYRRLFAKLDELSARLENQRFLVGDTITEADI
                     RLFTTLVRFDPVYHGHFKCNREKLAEMPVLWAYARDLYQTPGFGDTVDFDHIKRHYYQV
                     HEQVNPTRIVPNGPDLSGWATPHGRESLGGRPFGDGTPPGPPPAAEEIDRR"
     gene            16047..17204
                     /locus_tag="HUW46_RS29335"
                     /old_locus_tag="HUW46_05819"
     CDS             16047..17204
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336979.1"
                     /locus_tag="HUW46_RS29335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05819"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_215542015.1"
                     /sec_met_domain="DAO (E-value: 4.8e-64, bitscore: 216.3,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSLPATADIVVVGGGIIGVSCAHRLAESGVDVLLVDSGPLGAGS
                     TAKAAGGIRSSFTSRVNIELGLAGLEEYAAFESRYGVEIDFRRDGYLYLLTDPAELPEF
                     ERGVALQNSYGVHSRLVGVAEAKRVSPLIDTDGVVAAVWSPDDAKATPDSAVQGYARAA
                     RAHGATLCTGVTVDGIDRDGDEITGVRTSAGRVRTSAVLCAAGAWSRAVGAFAGVDLPV
                     EPHRRQVVFTGPVAGIPDSVPLTVEMPSAFYFHREGDGLAMSFPDPRARPGFDTRYEAG
                     TWLPRLAELAQRRVPAVLDAGVKTAWAGLYEMTPDHNQLVGEAAGVSRFLYATGFSGHG
                     FQMGPAIGRVVRDLFLGRQPAIDVAELDVRRFADRTATAAEHNIV"
     gene            17265..18878
                     /locus_tag="HUW46_RS29340"
                     /old_locus_tag="HUW46_05820"
     CDS             17265..18878
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150525.1"
                     /locus_tag="HUW46_RS29340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05820"
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="WP_215542016.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 3e-51, bitscore:
                     172.5, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1e-27, bitscore:
                     95.9, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDTGADRLADALARLGVEVAFGLPGVHNLPVWEALAERRLIGVR
                     HEQTAGYAADGYARTTGKLGVALVTTGPGAANTLGAVGEARASGSPVLVIATDIPSTLR
                     KPGVVRGVLHETSDQAALFAPVTKGTFTVGTADEIGAVVLEAAALALRPQSGPVYVGIP
                     TDLLRHPAGEIGAPPEPEPLPVPSEVDIQRAREVLASAERPLIWAGGGALRAGAGTEIG
                     ALAQRLGAPVVTTFAARGLLPPDHPCLAPNPVHAPEVGALWDGADVVLGIGTDFDGLMT
                     QNWLMPAPPRLIAVNVDAADAAKNYAPDLTLAGDAREVTARLADGLPPKAQPPLDDVKS
                     QVRQRIETEEPQAAVLLDALAELDPGAVVVADMCVAGYWAGGFHRVREPRTFAYPMGWG
                     TLGFGFPASLGAAASGARTVCVTGDGGFLYGCGELTTLAQERLPLTVLVVDDGGYGMLR
                     FDQDQEGVAHRGVDLVTPDFVALARSFGIPGVRVEGFGDEFRAKWTELTAGRGPNVLVV
                     GAALRPPVNTSPRWHRKGAR"
     gene            18875..20917
                     /locus_tag="HUW46_RS29345"
                     /old_locus_tag="HUW46_05821"
     CDS             18875..20917
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229602.1"
                     /locus_tag="HUW46_RS29345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05821"
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="WP_215542017.1"
                     /transl_table=11
                     /translation="MRIGVDIGGTFTDLCVLDETGVVAVGKVLTTHDEPARAVEEVLRA
                     TLKDAAPVTQLVHGTTLVTNALIERKGSRTALLATAGFRDVLEMRREHRYELYDLRIDL
                     PEPLVPRRLRFDVPERILADGTVDTPLDEDYVARLGRELAERGIEAVAICFLHAFTDSR
                     HERRAAEILREVAPGLRVALSSEVVPEIREFERASTTTANVYVQDLTERYLRDLEKRLH
                     GAGIGPAPHIMLSNGGIATVDTAARHPIRILESGPAGGALAAAAFGASAGEADLLAFDM
                     GGTTAKLCMISGGAPLVTHEFEIGRVYRLLPGSGLPVKVPVTDMIEIGVGGGSIARIDP
                     LGLLTVGPDSAGSEPGPVCYARGGSEPTVTDADLVLGYLDPAYFLGGAMSLDAAAAREA
                     IRTRIAEPLGVSVEQAAYGIHASVDEDMANAARVHAVERGKNPAEMPMFAFGGAGPVHG
                     AGVAKALGAPRVVAPPAAGVMSALGFLTAPLAFDFVRSARSGVDELTGAEVDALFAAME
                     AEGEALLTESGVDSADIVHRRIAEMRYTGQGYEIRVEVPEISADWPEPLVEAFTRTYRE
                     LYRRTGPDVGAEVLNWRVVSSGPVPEVALRLHGEETGEAKKGTRPAYFPEAGGFVDTPV
                     FDRYALRPGDGFTGPAIVEERESTLVVPPGARCAVRADASLEVTV"
     misc_feature    21060..21074
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
ORIGIN
        1 gtcaagcagt ccgggctcgg gcgcgagggc ggcgccgtcg gcatcgacga gttcctggag
       61 atcaagtaca tcgcggtgag cctgtgaccg actaccgcat cgcgagcatc cccggtgacg
      121 gcatcggcgt cgacgtcacc gtcgaggcgc gcaaagtgct cgacgccgcc gcggcgaaac
      181 accggttcgg tctgaagtgg acggagttcg actggagctg cgagcgctac gcgcaaaccg
      241 gcgcgatgat gccggaggac ggtgtcgagc agctctcggc gttcgacggg atcttcctcg
      301 gcgcggtcgg cttccccggg gtgccggacc acgtttccct gtggggcctg ctgatcccgg
      361 tgcggcgggc gttcgcgcag tacgtgaacc tccgcccggt gcggctgctc cccggcacca
      421 cttcggtgct ggccggtcgt tccgccgacg agctggagat ggtgatcgtc agggagaact
      481 ccgagggcga gtactcgacg gtcggcgggc ggcacaacaa ggggcagcag ggcgagttcg
      541 cgttgcagga gtcagtgttc acccgcgtcg gggtggagcg gatcatccgg tacgccttcg
      601 aactcgcgaa gacgcgcacc ggcagggtgt gctcggcgac gaagtcgaac ggcatcatcc
      661 actcgatgcc gttctgggac gagatcttcg cggagatcgc cgccgagtac ccggacgtcc
      721 acagtgaaca gtgccatatc gacgcgctgg cggcccggat ggtgcaggcg ccggaccggc
      781 tcgacgtcgt ggtcgggtcg aacctgttcg gggacatcct gagcgatctg gccgcggccg
      841 tcaccggcgg gctcggcatg gcgccctcgg gcaacatcaa cccgcccggc gaatacccgt
      901 cgatgttcga agcggtgcac ggcagcgcgc cggacatcgc gggccagggc atcgcgaacc
      961 cggtggcgca gatcctcgcc ggcgcgatga tgctcgacca cctcggtgag aaagcggcgg
     1021 cgaacgacgt caacacggcg gtggaaaaag tgctttccga agggaccgtc gcgacgccgg
     1081 acctcggtgg caagtcgacc accaccgaac tcggcagcgc gatcgcggaa gctctctgac
     1141 gcgatcgaaa gggccccggc cggaacgtcg attttctcgg cgaaccggtt ccggggccct
     1201 tttcggtatc cggttcgccc agccggatcc acgtagactc tcgtagctgc ccgccagcac
     1261 tcgcccggat ccctcgacct cgcaatgcct tcgccgtacg aactctacct ttcgttcgcc
     1321 gggggacccc cccgatttca acattaccgc gtgccaccga caatttcggg ggccgcctag
     1381 tgttccgtga ttctggtgtc tttactttgc gttgttgtcg acgagtttct tgatgttggt
     1441 ctggacgagt cggaccttgg cgaggatctc gtcggcggtg gcggtccagg tgaagggtgt
     1501 ggcggtggtg ttccagtgcg tgatgtagtc gcggatctgc ttgatcagga ctttcacgct
     1561 ggtgaaggtg ccgcgacgga tggactggcg ggtcatgatt ccgaaccagg tctcgatctg
     1621 gttgatccag gaggatccct tgggcgtgaa gtggaaccgc acgttcgggt tatcggccaa
     1681 ccaggcctgg acctccgggg tggtgtgggt cgagaggttg tccagcacga catgaacttc
     1741 cttgtctgcg tgcggtttca ctgccttctt cacgaacgcc aggaaccgca caccgtcgcg
     1801 gaccggcgcg cactcgccca cgacctcgcc ggtgcccacg ttcaacgcgg cgaacaagtt
     1861 cgtggtgccg tgccggacgt agtcgtgggt gcgttgctcg ctggcgtcga agtcgatggg
     1921 cagcagcggc tgcgtgcggt ccagggcttg gatctgggtc ttctcatcga tactgagcac
     1981 gaccgccgcg ccgggcgggt gcaggtagag cccgacgata tcggcgacct tctcggcgaa
     2041 ctccgggtct ccgctgacct tgaacgtgcc ctgcttctgt ggtcgaagac cgttctcgcg
     2101 ccacagcttc gccacgtaat gatgcgacac ataccgaccc tcggtccgga cgatgaacgc
     2161 ggccatctcc cggctcgacc agtgcgacag ccctgtctcg accggcgggc tcatcctgct
     2221 caccgccagg atccgagccc ggatcgccgc gggcacctgc tcccgcggag caccccgggg
     2281 gcgatccagc aacccggcga tcccttcagc gccgtaccgg cccagccaca gatccaccgt
     2341 cggccgcgac accccagcca acaccgcgac gtcctgcttc agccggctct ccgcgcgcca
     2401 caacacaatc cgcgcccgcg tcgccaccgt cgccggcaca tccgcgctgt tcagcaacgc
     2461 ccgcaactcg gcacgctcgt ccaccgtcaa ctccacccga cgagtaaacc ccacacccca
     2521 tcaacacaac gtaaagccaa cggaatcaca gaacactagt ctcggggacg gcgaaatcgc
     2581 gcgctcgctc gtaaaaccgc acgaacggtc cgttcgcgcg gttttcccgg cggatttccg
     2641 gtttttgtcg gcgcttcccg cgatagtggc tgtcatcgac cacggagggg agcacccatg
     2701 tgctggcaat gcgacaaccc gggaaagagc gtcggcgact acctcgacta catcgccggt
     2761 gaaataacca agtacggctg gatcgtccag tcggtggagg aggacgcggt gcgcgcgagc
     2821 tgggcttaca cggtcggcct caccaggacg gggcggccgg agctggtggt gaccgggctg
     2881 cccggcgagg tctccgccgg gctcctcaac gagttcgcgt cccatctcgg ccacagcgac
     2941 gacacaccgg tgccgggcga gcgcatcacc ttcgaaaacg gcgggccgtc gatcgagctc
     3001 gtcgagctgg ccgagccctc ggtgcatctg ctgatggccg tgggcctgta cggcaagaag
     3061 atccgcggcc tgcaactggt gtacgccgac gacgaaggcc gcatgccgtg ggaacgcgcg
     3121 tacaacgacg gccaaggcgg gcagcccgtt ctcggcagcc gcgcgaccgc tccgcgcggt
     3181 gacgatccct gaagagccga ggggctactt cccgccgcgc ccggacttcg ctgccgcgcg
     3241 gccggatgcc gcgtgcgcgc cgtcgctggt caccagcagc tcccgttcgt ccactgtgga
     3301 cacgtcgacc ggcccgagcg gtgcgggcgc ctggccggac tgcaggctgt tgccgggggc
     3361 gagccgctgc accgcgtcct ccatgtgcgc ttcgaggagg gagagcacgg cgtcctcgtc
     3421 accggcgcgg atcgcctcga tgatctggtt gtgctcggtc acccgctcct cgatgccctg
     3481 gtaggtgttc tgcagcgcgg acaggcacat cctggtctcg atcagcaggg tgcgggccat
     3541 ccggaccagc cgcttgctgc cggaggcggc gatcagcgct tcgtggaagc gcagatcggc
     3601 ctcggacaac gcggtgggat cgtcctgctc ggccgccgtg gccatctcgt gcaccgattc
     3661 ggcgagcacc gcggcgacgg cctcgcggtc gccgcggacg atccggagcg cggccgcgcg
     3721 ctcgatcgcc gagcgcgcgg tgtagatgtc gtagacgtct tcgggcccga ggtcgatcac
     3781 gaacaggccg cggtggcgct cgctgcgcag cagcccttcg gacaccaggc gctgcatcgc
     3841 ctcgcgcagc ggaccccgcg acacctggaa ctgcgcggcg agctcggtct ccccgagctg
     3901 ggtgccgggc tgcagcgagc cgttcatgat ggcgtcgcgc agctggcggg cgatgatgcc
     3961 cgccgtcgat tccctgctga ccggttcgat atcggcaagc ggcacggtca ttcgccccct
     4021 ctgcggaaca gtgtgccgaa cgaatctacg gtcttcgttt ccccggccag ccgcagccct
     4081 tcccagatgg tgacctggtt ggccgtgagc accggtttgc cgagccgttc ttcgatggcg
     4141 ccgagcacgc cgagggtgcg catcgcggtg tccggcacga gcaccgcttc ggcgtccgga
     4201 tggtcgtgcg acacggccag ttcgaccacg gcctcgggcg acagcctgcc gacctcggcg
     4261 gccgtgtcga tgtcttcgct cgacatcgaa accacttcga catcaccgga agcgaggaac
     4321 tcgacgaaca gcttcgccac gtcatcgggg tagctggcgg cgacggccac cttccgcaca
     4381 ccgagtgcgg cggcggcgtt gacgaacgcg aaggaggtgc tggacgcggg cttacccgct
     4441 gcttcggcga gcttttcgac ctgttcacgg gcaccgtcgg ggcggtagac gaagctgccg
     4501 ctcgtgcagg cccacaccac ggcgtccggt tcgtgcttcg cgagcaggcg cgcgccttcg
     4561 gcgagccgtg ccgggctccc gagatcgagc agttcgggca cggcgtgcag gtcggtgccg
     4621 tagatgtgct ccacgggcaa ccgcaccccg gccccgagca caccctcggc cagcgggtag
     4681 tcgtcctcgg ccgcgtggtc gggatagatg aagccgatga ccggcaaggc ggacctcctg
     4741 cgacgctggg gctgtagatt gtcgacaatc ctaacggaca agtcgagcgc ggtcacgtgt
     4801 caactgacct cccgcagcca gttgccgggg ccgacgatcg gcagccgcat ccggttgagg
     4861 caggcccaca tggtgagctg gttcgcggtc agcacgggtt tgccgagcgc ggtttcgagc
     4921 ggttcgatca ggtcgtaggt ggggaggttg gtgcagctga cgaagatcgc ttcggcgtcg
     4981 gggtggtcgg ccttcaggat ccgctcggcg atcgtgcggt agctgacctt ccagatgccg
     5041 ccgccgagcc cgaggtgctc gctcgccacg gtttcgacgc cgagttcggc gaggaaccgg
     5101 tgcaggctgc cggtgagatc ggcgtcgtag ggggtgatca ccgcgatgcg cctgctgccg
     5161 agctgctgta gcacctcggc gagcgcgccg gaggtggtca ccgcgtccgg ggcgcccgcg
     5221 tcgcagatcg ccttcgtgag cgaccgttcg tagtcgacgc cgttgacgaa gctgcccgag
     5281 gtgcacaggt aggcgaccac ctccggctcc acgtgcagca cgtcgcgggt ggcggtggcg
     5341 aggtgggcgc tgttgctgac cagctgcgcc atctccatgc tgaccggcac cggctcgtac
     5401 ggggtgcgcg cgaggtgcag cgacacttcc atcggcaccc agcgccacag ctcgcgctcc
     5461 agtgcgaggt cgaacggcgc gatcacgccg atgccccgct gggcgagcgg cccttcgaat
     5521 tcgggcaacg agaaatccaa ggctccagcc tccggcgaac gatgtggaag gagtgcctcc
     5581 ggggtggctc ctcggattgt tgacaatcat acgaccggct cataccgtgt caatgtgatc
     5641 ggcacggatt ccccggtgct ggcggtgctg tgcggcgaca agcacccccc ggacatgcac
     5701 agcatcgaac aggccgccga ggttcgttac accggcgccg acggcctggc cggcgcgctg
     5761 gccggagccg acgcgctgtt cgtctacgac ttcctctcca ccgcggtgcc cggcgcatgg
     5821 cacgcggccg accggctgcg ctggctgcac atcgcgagcg cgggggtcga tcccgtgctg
     5881 ttccccgggc tggtggacag cgaggtcgtg ctgaccaact cgcgcggcgt gttcgacgat
     5941 tcgatcgccg agtacgtgct cggcgtgatc ctgaccttcg cgaagggcat cgcgcgctcg
     6001 ctcgacctgc agcgggaaaa ccggtggctg cacagggaaa gcgagcggat cggcggcaag
     6061 cgggcgatcg tggtcggcac cggtccgatc ggcaggtcga tcgcgcggct gctgcgggcg
     6121 gcgggcatgc gggtgtccgg cgcggggcgg cgggcccgcg acggtgaccc ggacttcggc
     6181 accgtccact cctccgaggc gctcgccgag cagttgccgg aagccgacta cgtggtcgcg
     6241 gtggcgccgc tgaccgagcg gaccaagggc atgttcgacg cgaaggcgtt cgcggcgatg
     6301 aaaccgtccg cgcggttcgt caacgtcggc cgcggtgagc tggtggtgac cggggacctg
     6361 gtcgccgcgc tgcgggacgg gcagctggcc ggtgcggcgc tggacgtgtt cgagaccgag
     6421 ccgctgcccg ccgagagtcc attgtggacc atgccggaag tgctggtgtc accgcacatg
     6481 tcgggtgact tcgtcggctg gcgcaacacc ctcgtcgagg tgttcgcgga caacttccag
     6541 cggtggcgcg agggcgtgcc gcttcgtaat gtcgtggaca agaaactggg ttacgtgccg
     6601 tcctcgccct gacgcgagac gacggaccag cgaaccgggc gcaccggcga acaaggagaa
     6661 ccatgcctgc caacaccggc accctgctga ccgcgagtga actcgtcgcc gcgtactcga
     6721 cgggcgaact gtcgccggtg gaggcgacgc aggcggcgct gaaggcgatc gaggaccgcg
     6781 acggcgacct caacgcgtac tgcctcgtcg acgcggaggc ggcgctcgag caggcgaagg
     6841 gctcggagga gcgctggcgg cagggcaacc cgatcggctg gctcgacggc gtgcccactt
     6901 cgatcaagga catgttcctc acccagggct ggccgacgct gcggggctcc aaggcgatcg
     6961 accccgacca gccgtgggag gtggacagcc cggtcaccgc gcggctgcgc gaaaacgggc
     7021 tggtgctgct gggcaagacc acgacaccgg agctcggctg gaagggcgtc acggacaacc
     7081 cgctcaccgg gatcacccgc aacccgtggg acaccacccg caccgcgggc gggtcgagcg
     7141 ggggcagcgc ggccgcggtc gcggccggga tgggcgagct gtcggtgggc accgacggcg
     7201 gcggttcggt gcggatcccg gcgtcgttct gcggcatcgt cgggctcaag ccgacgcacg
     7261 gccgcatccc gctgtacccg gcgagcccgt tcggcgcgct cgcgcacgcc gggccgatgg
     7321 cgcgctcggt cgacgacatc gcgctcctgc tcgacgtgct cgcactggcg gatttccgtg
     7381 accccgcggc acttccgccg ctcgcgggcg actaccggtc ggccgtccgc cgcgacgtcc
     7441 gcgggatcaa cgccgcgttc tcacccgatc tcggctacgt caaggtcgac ccggaggtcg
     7501 ccaagatcgt cacctccgcg gtcgccgcgc tcaccgaggc cgggctgaac gtcaccgaga
     7561 ccgcgcccgg tttcagcgac ccgatggacg ccttcgacgt gctgtggtcg accggcgcgg
     7621 cgaagtggct ggacagcttc cccgagggct cggcggaggt catcgacccc ggtctgcgga
     7681 aggtgtggga aaagggcctc acctactccg cgagcgatta cctcggcgcg aacgcggagc
     7741 gcgccgcgct cggcatccac atgggcgagt tccacacccg ctacgacgtg ctgatcaccc
     7801 cgaccatgcc gatcgtgccg ttcgaggccg gtgtcgacgt gccgccgggc agcgggctga
     7861 gcagctggcc ggagtggacg cggttcagct acccgttcaa catgacccag cagcccgcga
     7921 tcagcgtgcc agccgggttc accgagggcg ggttgccggt cgggctgcag atcgtcgggc
     7981 cgcggcattc cgacgatctc gtgctcgcgg tggcgaagct gctggaggag atccgcccgt
     8041 ggccggtcga ccggccggcc ggggtgctcc gctgaccggc accgccggat gcatcgatcc
     8101 agagcctgtg gtgtgctgga ggcatgcgta ggacgatttt gggcaagagc ggcctcgacg
     8161 tctcgaggat cgctttcggg acctggcagc tcggcggcga ctggggctcg ttcgacgagc
     8221 agaccgccgt ttcggccatc cagcacgcca gggaactcgg cgtcaacttc ttcgacacgg
     8281 cgcaggccta cgggttcggc aagtcggagg ccctgctcgg caaggcgctc cgcgacgagc
     8341 tgaagcgtga ccgcgacagc ctggtgatcg ccaccaaggg cgggatcaac cccggtggcg
     8401 agcgcccgcg cgacgcgggc cgggcgtgga tccgcaaggg cgtcgaagaa agcctcgagt
     8461 acctcgggct cgaccacatc gacctgtacc aggtgcactg gcccgacgcg gacaccccgg
     8521 cggaggagac cgcgggggtg ctgcaggaat tggtcgacgc gggcaagatc cgccacgccg
     8581 gggtgtcgaa ctacgacgcg gcgcagctcg ccgacttcga ccgcacccgc ccggtcgaga
     8641 cgcttcagcc gccgtaccac ctcttccgcc gccacatcga gaccgacccg ctgccctacg
     8701 ccagggagca cgaaatcggt gtgctggcct acagcccgct cggcagcggg ctgctcaccg
     8761 gttcgctcac cgcggacgcc acgttcgaag cgagcgactg gcgttcgaag gccaccgcgt
     8821 tcgggggcga cagcctgcgc cgcaacgtcg aagccgtcgc cgaactggcc gagttcgcca
     8881 agaccaagga cgccacggtg agccaggtgg cgatcgcgtg ggtgctcgcc cagcccggcg
     8941 tgcacgtcgc gatcgtcggc gcccgcaagc cgaagaacat cgaagacagc ctcgccgccg
     9001 cggatctcga cctgtccgcc gacgacctgg ccgagatcga gcggatcacc gccaagggcg
     9061 tgcaggtgga cggcgccagc ccggagggcg tcgcctgagg gcggccgggt ccgtcacagc
     9121 gtgagcaccg tgccgtcgtg cgggatttcg accccggcgg cgagcacctc ctggtgcgcc
     9181 gcggaatcgt ggtccaccaa ggggttcgtg ttgttcaggt gcgtgtaggc ccagcgctgg
     9241 tccggcgcga gcacgagctg ccgccggatc agcgccaggc tgccgtccgc cccggaaacc
     9301 ggcaggtgcc ccatcgcgcg ctgggcgccg tcggcggacg agccggtggc cccggacatt
     9361 tcgccgccgc cgtggaacgt cccgtcgagc agcacgaagc tcgcgccgcg cacgaagtgg
     9421 tcgaaaccgg gtggccacga cgccaggcac ggcgcgtaca cgagcgagcc gcccgtcacc
     9481 gggtcgtcga accggtacgc caccacccac ggaccgtcca ctgtggatga tgtggcgtac
     9541 ttcgggcgtt tggtgccgac cggcagcacc gacactcgca gcccgtcgat caccgcaccg
     9601 gaggagagcg ggcgccaagt ccactcgccg taccccgcca cgacggcctg catcggcagg
     9661 gcgtggcgcg tcgcgtgcag cacggcgtcg ggcgcccaca cggtcatgcc cgcgccttcg
     9721 cgcagcatca gcaggcccag ggtgtggtcg agttcgccgt cggtgaggaa agcgccgcgc
     9781 agcggggttt ccctcgggcc ggggcccggc gccagctccg gggtcgccag cacctgggcg
     9841 cggatgtcgg gggaggcgtt gaccaggtac cagcccttgc cgtcggcact gaccgcgagg
     9901 cagtcctggg tgcgcggcgc cgccccactc cggcacagcg cgcacgcgca gttccactgg
     9961 gggaagccgc caccggccgc ggtgccgagc agtgccacct tcatcgcgtg acctcccgca
    10021 tgaccagcgg cggttcgtgc cggggtgcgg cgaggacgag atcgacgacg ccgcggtccg
    10081 gcgagcggga gcacaccgga tccgtcgccg ccgcgtcacc ggtgagcagg aacgcctggc
    10141 accggcagcc gccgtggtcg aggccgcgcc gatcgcacgt gcggcacggc tcgcgcatcc
    10201 agtcctcgcc gcggtaggcg ttgaacgacg tcgagtcgta ccagatctcc gcgagcggcc
    10261 gcttcgtgac gttgtccagt tcgagcgtgg tgatcgcggt cgcggcaggg caggggagca
    10321 cgtcgccgtt cggcgcgacg gtcagctgcc gcgcgcccca gccgtacatg cacggcttcg
    10381 ggtacggctc gtagtagtcg gcgacgacgt agatgatctc catccgcccg cgcagccgct
    10441 cggccgcggc gcgcacgacg ggttcggcgg cggcgagctg gtccggggtc ggcatcagcg
    10501 cgtcgcggtt gcgcagcgcc cagccgtagt actgggtgtt cgccagttcg agccggtcgg
    10561 ctcccatttc ctcggcgagc gcgatgatcc cggcgagctg gtcgtgattc tgccggtgca
    10621 gcaccacgtt cacgctcagc gggaggtcgt gggccttgac cagctcggcc gccctgatct
    10681 tgtggtggaa cgccttggcg ccggcgaggc ggtcggcgcg ctcggcgtcg gcgccctgca
    10741 cggacagctg gatgtgcgcg atgccccggt cggcgagatc ggcgagccgg gtgtcggtga
    10801 gcccgaggcc gctcgtgacg aggttgacgt agcagccgag cccggtcgcg tgctcgacga
    10861 gccggggaag atccgggcgc gccaacggtt ccccgccgga catgtggacc tggagcacgc
    10921 cgagctcgcg agcctgggtg aggatggaca gccagtcctc ggtgcccagt tcggcgtcac
    10981 gggtgagaag ttcgagcggg ttcgagcagt aggggcagtg cagcgggcag cggtgggtca
    11041 gctcggcgag cagccccagc ggcggttcga tcacgtccac cggaccacct gccgatcggc
    11101 cagtttttcc agcagggcaa cgacttcctg cgtgcgcacg ccggagtacc gctcaccgag
    11161 cgatgcggtg atctcctcga cggtggcttc gccgtcgcag agcccgagga ccgcggcggc
    11221 ggtcgcgttg ggcaccagca cgccttccgg gaagagcagc acgtgggttt ctcttgtccg
    11281 gtcgtaggtg agccggactc cggtgcgcag cttgggtttc gccatgatca gtccttttcg
    11341 cacgcgcgct cgaccgcgtc caggatggac cacagcacgt cgcacttgaa ggagagcgcg
    11401 gcgatcgcgg cctcctgctc ggtccgggtg gtcgagtacc gcacgactat gtccactgtg
    11461 gcctgtcctt cgccggagac cgcgtcgatc cggttcgtga agtagtcgag gtcctcggcg
    11521 cggatccacg cgtagttcgc cagcatgtcg ctcaccctgc ggcgcatgag atgaccggcg
    11581 aacatctcgg tcagcccgga cgcgaccgct tccacccacg gtctcgtccg cgcgaaggtg
    11641 acgtaggcgt cgacggcgaa ccggacgccc ggcgcgaggt ggcgctcgtc gagcacctct
    11701 tcgcgggtca ggccgaccgc ctggcacagg cgcagccagc gctcgatacc gccttcgccc
    11761 tcgcgagcgc cgtcgtggta gacgatccgg tcgagccagt gccgcctgat ctccggcacc
    11821 gggcagttgc tgatgatcgc cgcgtccttc cgcgggagca cgcactggta gtaccagcgg
    11881 ttcgcggccc agatcttcag ctcccgctca tcgagatcgc cgtcgtgcag ccggtggtgg
    11941 aaggggtgtg ttccccagta ccgatgggac agtccgcgca gcgcggcgac gaattctgtt
    12001 tcactgagcg gagcactggt ggtcatgatg tcctcctcgg ccggtgtcac cggaggaggt
    12061 ggagggcctg gcagaccctc caccccgcac tcctagtcct ccatgcgggc cgcgtacgcg
    12121 gtgacctcca tgggggtttc gtactccacg aagtccggtg tggtccagac ctcgacctgg
    12181 gtttccttca tgtgcactcc tctgatcacc ggcggctcga ctctcgccgc cgcccaaagg
    12241 gccggatagg gcacgtcgac cctagaagct cacgcgcccg gatatccaga gcggaaagga
    12301 ggttctcttg ccgcttggca caggttcgct ggtctggtcc actacttcgt aaaaattatt
    12361 acctaacgga gtcataatag atctgatgta cgatgtgcta tgcacccaga cggtggtgtc
    12421 ggctccggcg ccagaggtgg ttctatgcgt ccaggtggcc ccgccgcagg ggcaccccgc
    12481 cgccggaaca cccacccgac ttctggaggc tccatgtccc tgcgtcgaag cgccgtcttc
    12541 gcggccgcgt tcgcgttcgc cgcgctgacc gtcggcggcc ccgcggtgac ctccgccgcg
    12601 ccagccgccg caccgaagaa ggtcgcgagc ggcctgaacg aggcctggtc cgtcgacttc
    12661 ctgcccgacg gcaccgctct gttcaccgag aaggactcga agaagatccg caagatcgac
    12721 aagtcgggca aggtctccga cgtgcagacg gttcccgggg tgagcgtcac caaggaggcc
    12781 gggctgctcg ggatcgcggt ttccccgcac tacgcgcagg accagaccgt ctacgtctac
    12841 tacacgacga gctcggacaa ccggatcgcg aagctcaagc tcggcgggga cccggtgccg
    12901 atcctcaccg gtatcccgcg gggcacgcag ttccaccagg gcgggcggat caagttcggt
    12961 cccgacgggt tcctctacgc gggcacgggt gacgcgaaga agagctccaa cgccccggac
    13021 aagaactcgc tcggcggcaa gatcctgcgc atcaccaccg acggcaagcc cgcgccgggc
    13081 aacccgttcg gcaaccccgt ctactcctac ggccaccgca acgtgcaggg cctgacctgg
    13141 gtcggcgacc agctctacgc ctccgaagtc ggggagagca cctacgacga gctgaacaag
    13201 atcgaggcag gcaagaacta cggctggccg aagtgcgagg gcaagtgctc ggagccgggg
    13261 atgaccaacc cggtcaacca atggtccact tcggacgcca cgccgagcgg aatcgccaac
    13321 tacaaaggaa agctgtacat ggccgcgctc aagggcggca cgtacaaact ggacaccgcg
    13381 ggcaagggcg ggaagctcta caccgatctc ggccgcaccc gcgacgaggt ggcgggcccc
    13441 gacggcaggc tgtggatcgt gacgccgggc gccatctaca ccgcggacgg caactgagtc
    13501 cttttcggac tccggaattc ggtggtggcg cgtcctttcc cgggggaagg gacgcgccac
    13561 cgcgtcagcg ggtcgcgaac tccagctcgg ccaaggcggt gtccaggtgg gtcaggatgc
    13621 gctgcaggtg cggcacgctg cggcggcacc cggtgatgcc gaagtcgagg ttgtcgccgt
    13681 tgctggtgag ggtgatgttc agcgcctgcc cgtcgagcag gaccgacgcg gggtagatgc
    13741 cgtcgaggcg cgcgccgttc cagtacatct gggaccgcgg ccccggcacg ttcgagatca
    13801 ccaggttgaa cggcggcttg gtgttgttga cgacgccggg gatcggcgag gcgccgagcg
    13861 cggcgacgtt gatcccggac agcagcagca cctgcaacgg ggtgagctgc ttgaacatcc
    13921 gcttgccgtc gcgcatcgac ttgtggatca ccgcgagccg gtcggccggg tcggccttgt
    13981 cggtgccgag gttgcacagc agcgcgccga tgttgttgcc cgcggcttcg ctgtcgtcgg
    14041 tcttggcccg cagcgacacc gggaccatcg cggtcatcgg ggcgtccggc agcgcgcgct
    14101 gttcgatcag gtagtcgcgc agcgcgccgg agcacatcgc cagcacgacg tcgttgcggg
    14161 agaccccagc ggcggtggcg atggcgcgca cgcgttcgag agaccacgac tgcgcggcga
    14221 accggcgggc gccgccgatc ggcacgttca gcatcgtctt cggcgcctgc agcggcaggg
    14281 tcagcgtgtg ctcgcggaac gcctcgttcg ccaccttgta cgcggcgggc gcgatcccgg
    14341 cgagctgact cagggttttg cccgcggtct tgaacaacga ggtgggcgcg cgcccgttgc
    14401 ggtgtccgtt gacctcgcgc gagccccacg gcggcgggca gtccaggtcg ccggggtcgt
    14461 cgctgagggt gccctgcagg tgccgcagcg cggagacgcc gtcgagcagc gcgtggtgga
    14521 tcttgctgta gatcgcgaac cggtcgtcct gcaggccctc gatgaggtgg atctcccaca
    14581 gcggccggtg ccggtcgagc agcgtgccgt gccagcgcga ggtcagttcg agcagttccc
    14641 tgatccggcc gggctggggc agcgcggaat gccggaagtg gtagtcgatg tcgagatcgt
    14701 tgtcctccga ccacgcgagg tgccccgcgg tgttgaccgg gttcgccggc ctgcggcgga
    14761 agatcgggcg catgttcgtg tcctcgcgca gccgctgccg cagctcgctc aggtactccg
    14821 ggcccgcgcc ttccggtttc tcgaacagtt cgagaccgcc gacgtgcatc ggatgttcgc
    14881 gggtctccac cagcaggaac atcgagtcgg tcacgggcat caacggcatg gcgaccacct
    14941 catagtcctt cgtcgagtgc cgagagtcta agcacattgt cggcacgggg aaaaagcccg
    15001 ctgctccaaa cgagaaagcg cgacgcggcg accgcgccta tcgtcgaggg catgaccatc
    15061 ccgaccgatc cggagacggg cgagttccac cgctccgcca accacttcga cgcgcgcatc
    15121 accgccgacg gccgcgacgg ctggccggtc gaacccggcc gctaccggct ggtggtgagc
    15181 cgggcgtgcc cgtgggcgag ccgcgggttg atcgtgcgcc gcctgctcgg gctggagcaa
    15241 gcgctttcgg tcgccgtcgc ggacccgatc caggacgaga agagctggcg cttcaccctc
    15301 gatcccggtg accgggatcc ggtactgggc atcaagtacc tcggcgaggc gtacttcgcg
    15361 gcggaaccgg gttacgacgg cggcatcagc gtgcccgcga tcgtcgacgt gccgagcggc
    15421 aagctcgtca cgaacgacta tccgcggatc acgctcgacc tgtccacgga atggacggcg
    15481 caccagcggg aaggcgcgcc cgagctgtac ccggaacggc tgcgcgacga gatcgacgag
    15541 gtcaaccgcg gggtgcacgc cgacgtcaac gcggccgtct acgaagcggg tttcgcgagc
    15601 aagcaggccg cttacgacga cgcgtaccgg cgcctgttcg cgaagctgga cgagttgtcg
    15661 gcccggctgg aaaaccagcg cttcctcgtc ggcgacacca tcaccgaggc cgacatccgg
    15721 ctgttcacca cgctcgtccg gttcgacccg gtctaccacg ggcatttcaa gtgcaaccgg
    15781 gaaaagctcg cggaaatgcc ggtgctgtgg gcttacgcgc gcgatttgta ccagactccc
    15841 ggcttcggcg acaccgtcga cttcgaccac atcaagcgcc actattacca ggtgcacgag
    15901 caggtcaacc cgacgcggat cgtgccgaac ggcccggatc tgtccggctg ggcgaccccg
    15961 cacggtcgcg agtcgctcgg cggcaggccg ttcggggacg gcacgccgcc gggtccgcca
    16021 ccggcagcag aggagatcga ccggcgatga cgagccttcc cgccaccgcg gacatcgtgg
    16081 tcgtcggcgg cgggatcatc ggcgtgagct gcgcccaccg gctcgccgag tccggagtgg
    16141 acgttctgct cgtcgactcc ggcccgctcg gcgccgggtc gacggcgaag gcggcgggcg
    16201 ggatcaggtc gtcgttcacc agccgggtca acatcgaact cggtctggcc gggctcgaag
    16261 agtacgcggc cttcgagagc cgctacggcg tcgagatcga cttccgtcgc gacggctacc
    16321 tgtacctgct caccgatcca gccgagctgc cggagttcga gcgcggggtc gcgctgcaga
    16381 actcctacgg tgtccacagc agactcgtcg gcgtggcgga ggccaagcgg gtgtcgccgc
    16441 tgatcgacac cgacggcgtg gtggccgccg tgtggtcacc ggatgacgcc aaggcgaccc
    16501 cggattcggc cgttcagggg tacgcgcgcg cggcgcgggc acacggcgcc acgctgtgca
    16561 cgggcgtgac ggtggacggc atcgaccgcg acggcgacga gatcaccggt gtgcgcacgt
    16621 cggcgggccg ggtgcggacc tcggccgtgc tgtgcgcggc gggcgcgtgg tcgcgggccg
    16681 tcggcgcgtt cgcgggcgtg gatctgccgg tggagccgca ccggcggcag gtggtgttca
    16741 ccgggccggt cgccgggata ccggattcgg tgccgctgac cgtggagatg ccgtccgcct
    16801 tctacttcca ccgcgagggc gacgggctcg cgatgtcctt ccccgacccg cgcgcccggc
    16861 cggggttcga cacccgctac gaagccggga cctggctgcc ccggctcgcc gagctggcgc
    16921 agcggcgcgt cccggccgtg ctcgacgccg gggtcaagac cgcgtgggcc gggctgtacg
    16981 agatgacgcc ggaccacaac cagctcgtcg gcgaggccgc cggggtgtcg cggttcctct
    17041 acgccaccgg gttttccggg cacggattcc agatggggcc cgcgatcgga cgcgtcgtgc
    17101 gggacctgtt cctcggcagg cagcccgcca tcgacgtcgc cgagctggac gtccgccggt
    17161 tcgccgaccg gaccgcgacg gccgcggagc acaacatcgt ctgaagcgct ctgccgttga
    17221 agcgctattg ctggatcgtt gaacgattcg gtaccgtcgg cccggtgacg gacacgggtg
    17281 ccgatcggct ggcggacgca ctggcgaggc tcggggtcga ggtcgccttc ggtctccccg
    17341 gcgtgcacaa cctgccggtc tgggaagccc tcgccgagcg aaggctgatc ggtgtccgcc
    17401 acgagcagac cgccgggtat gccgccgacg ggtacgcgag gacgaccggg aagctcggcg
    17461 tcgcgctggt caccaccgga cccggcgcgg cgaacacgct cggtgccgtc ggcgaggcgc
    17521 gggcttccgg ctcgccggtg ctcgtgatcg ccacggacat cccgtccacg ctgcgcaaac
    17581 ccggtgtggt gcgcggtgtc ctgcacgaga cgagcgatca ggcggcgctg ttcgcgcccg
    17641 tgacgaaggg gacgttcacc gtcggtaccg ccgacgagat cggggcggtg gtgctcgaag
    17701 cggccgcgct cgcgctgcgc ccccagagcg ggcccgtcta cgtcggcatc ccgaccgacc
    17761 tgctgcggca ccccgccggg gagatcggcg cgccaccgga accggagccg ctgcccgttc
    17821 cgtccgaagt ggacatacaa cgcgcgcgag aggtgctcgc gtcggccgag cgcccgctca
    17881 tctgggcggg aggcggtgcg ctgcgggcgg gtgcgggtac cgagatcggc gcgctcgccc
    17941 agcggctcgg cgcgccggtg gtcaccacgt tcgccgcgcg gggactcctg ccgccggacc
    18001 atccgtgcct ggcgccgaat ccggtgcacg cgcccgaggt cggcgcgctg tgggacgggg
    18061 ccgacgtggt gctcggcatc gggaccgatt tcgacggcct gatgacgcag aactggctca
    18121 tgcccgctcc gccgcgactg atcgccgtga acgtcgacgc ggcggacgcg gcgaagaact
    18181 acgcacccga tctcacgctc gccggtgacg ctcgcgaagt gaccgcacgg ctcgccgacg
    18241 gcttgccgcc gaaagcgcaa ccgccgctcg acgacgtgaa aagccaagtg cgccaacgga
    18301 tcgagaccga ggaaccgcag gcggccgtcc tcctcgacgc gctcgccgaa ctcgaccccg
    18361 gcgcggtcgt ggtcgccgac atgtgcgtcg ccgggtattg ggcagggggc ttccaccggg
    18421 tgcgcgagcc gcgtaccttc gcctacccga tgggctgggg cacgctcggg ttcggcttcc
    18481 ccgcgtcgct cggtgccgcc gcgtcgggtg ccaggacggt gtgcgtcacc ggcgacggcg
    18541 ggttcctcta cggctgcggc gagctgacca cgctcgcgca ggaacggctt ccgctgaccg
    18601 tgctcgtcgt cgacgacggc ggctacggga tgctgcggtt cgaccaggac caggagggcg
    18661 tcgcgcaccg cggcgtcgat ctggtcaccc cggatttcgt cgcgctggca aggagtttcg
    18721 gcattcccgg cgtgcgcgtc gaagggttcg gcgacgagtt ccgtgccaag tggacagagc
    18781 tgaccgccgg gcgggggccg aacgtgctgg tggtcggcgc ggcgctgcgg ccgccggtga
    18841 acacctcgcc gcgctggcac cggaaggggg ccaggtgagg atcggcgtcg acatcggcgg
    18901 cacgttcacc gacctgtgcg tcctcgacga gacgggggtc gtggccgtcg gcaaggtgct
    18961 gaccactcac gacgagcccg cccgcgcggt cgaggaagtc ctgcgcgcca cgctgaaaga
    19021 cgccgcgccg gtgacgcagc tcgtgcacgg caccacgctc gtcacgaacg cgctgatcga
    19081 acgcaagggt tcgcgcaccg cgctgctggc gaccgcgggg ttccgcgacg tgctcgaaat
    19141 gcgccgcgag caccggtacg aactgtacga cctgcggatc gacctgccgg aacccctggt
    19201 gccccggcgc ctccgcttcg acgtgccgga gcggatactg gccgacggca ccgtcgacac
    19261 cccgctcgac gaggactacg tggcccggct cggccgcgag ctggccgagc gcgggatcga
    19321 ggcggtcgcg atctgcttcc tgcacgcgtt caccgactcc cgccacgaac ggcgcgccgc
    19381 ggagatcctg cgcgaggtcg ccccgggact gcgcgtcgcg ctctccagcg aggtcgtgcc
    19441 ggagatccgg gagttcgaac gcgcgtcgac gacgacggcg aacgtctacg tgcaggacct
    19501 cactgaacgg tacctgcggg atctcgaaaa acggctgcac ggcgcgggaa tcgggcccgc
    19561 gccgcacatc atgctgtcca acggcgggat cgcgaccgtc gacaccgccg cgcgccaccc
    19621 gatccggatc ctcgaatccg gcccggcggg cggcgcgctc gcggcggccg cgttcggcgc
    19681 gagcgcgggg gaagccgatc tcctcgcgtt cgacatgggt ggcaccacgg cgaagctgtg
    19741 catgatctcc ggtggcgcgc cgctggtgac gcacgagttc gaaatcggca gggtgtaccg
    19801 gctgctgccc ggatcggggc tgccggtgaa ggtgccggtg acggacatga tcgagatcgg
    19861 ggtcggcggc gggtcgatcg cgcgcatcga cccgctgggc ctgctcaccg tcggacccga
    19921 ttcggcgggt tccgagcctg gcccggtgtg ctacgcgcgg ggcggtagcg agccgaccgt
    19981 gaccgatgcc gatctcgtgc tcggctacct cgatcccgcg tacttcctcg gcggcgccat
    20041 gagcctcgac gcggccgccg cgcgcgaagc gatccgcacc aggatcgccg agccgctcgg
    20101 ggtttccgtg gagcaggcgg cgtatggcat ccacgccagt gtcgacgagg acatggccaa
    20161 cgcggcgcgc gtgcacgcgg tcgaacgcgg caagaacccc gccgaaatgc cgatgttcgc
    20221 cttcggcggc gcgggtccgg tgcacggcgc gggcgtggcg aaagcgctcg gcgcgccgcg
    20281 tgtcgtggcc ccgcccgcgg cgggcgtgat gagcgcgctg ggtttcctca ccgcgccgct
    20341 ggccttcgac ttcgtccggt ccgcccggtc cggtgtggac gaactgaccg gcgccgaggt
    20401 cgacgcgctc ttcgcggcga tggaagccga aggcgaagcg ctgctgaccg agtccggtgt
    20461 ggacagtgcc gacatcgtgc accgcaggat cgcggaaatg cgctacacgg ggcagggtta
    20521 cgagatcagg gtcgaggtgc ccgagatcag cgccgactgg ccggagccgc tcgtcgaagc
    20581 gttcacccgc acctaccgcg agctgtaccg gcgcaccggc cccgacgtgg gggccgaggt
    20641 gctcaactgg cgggtggtgt ccagcgggcc cgtgccggaa gtggcgcttc ggctgcacgg
    20701 cgaggaaacc ggcgaggcga agaagggcac caggcccgcc tacttccccg aggcgggtgg
    20761 atttgtcgac acgccggtgt tcgaccggta cgcgctgcgc cccggtgacg ggttcaccgg
    20821 cccggccatc gtcgaggaac gggagtccac tttggtcgtt ccgccgggcg cgcgctgcgc
    20881 cgtacgggcg gacgccagcc tggaggtgac ggtgtgacgg acccgatcct ggtcggcgtc
    20941 gtcggcagca ggctgcattc gatcctcgcc gagcagcaga acgcgctcgt caacaccgcg
    21001 ttttcgtccg tggtaaggga atcgctcgac ctcgcctgcg ccgtgttcga ctcgcgcggc
    21061 gagatgatcg gccagtccat cggcggcacc ccagggcaca tcaacgcgat ggccaccggg
    21121 atgcaccact tcaccgccgc ctacccgccg gaggaactgg cgcccggcga cgtcctgctc
    21181 accaacgacc cgtggcagac cgcgggccag atcaacgaca tcaccgtggc gaccccggtg
    21241 ttcgcgcgcg gcaggctggt ggcgtggttc gcgtcgtgct gccacgcgcc ggacatcggc
    21301 ggcaggctcg tctccgcgga agcgggcgag gtcttcgagg agggccttcg cctgccgatc
    21361 atgaagttcc tgcgcgccgg cgaggtcaac gccgatctcg aacggctgat cagggcgaac
    21421 gtgcgcaccc cggaggagac gatcggcgat ctcctcgcgc aggtcaccgg caacgaggtg
    21481 ggcgcggcga gcctggtgcg cctgctggac gagttcggcc tcggctcgct cgacgaggtg
    21541 gccgccgaga tcatgaaccg gtccgaacgc gcgctgcggg acgcgctcgc gcggatcccg
    21601 gacggtacct acaccaacga aatgcgcacc gacgggttcg acgacgaaga ggtcgtgctt
    21661 aaggtggccg tcacggtgga cggcgaggac gtccacgtcg acttcgcggg ctcgtcgccg
    21721 cagagcaggc acggcatcaa cgtggtgctg aactacacgc gcgcgtacgc ctcgttcgcg
    21781 gtcaaggcgg cgatttcccc gcaggtgccg cacaacgcgg gctcgttccg gccggtgcac
    21841 gtgaccgcgc cggaagggtc gatcctcaac tgcgccgagc ccgccccggt cgcctcgcgg
    21901 cacctcatcg ggcacttcct gccgtcactg ctgatcggcg cgctgcgcca cgcggtgccg
    21961 gaaatcacca tggcggcgag cgcggacgcg ttgtggatgc tgatctggcg cggtaccggg
    22021 cccggc
//
