LOCUS       NZ_CP059997            10393 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Amycolatopsis sp. CA-230715 chromosome, complete genome.
ACCESSION   NZ_CP059997
VERSION     NZ_CP059997.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. CA-230715
  ORGANISM  Amycolatopsis sp. CA-230715
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 10363158)
  AUTHORS   Jorgensen,T.S., Gren,T., Oves-Costales,D., Ortiz-Lopez,F.J.,
            Carretero-Molina,D., Gonzalez,I., Blin,K., Genilloud,O. and Weber,T.
  TITLE     Complete Genome Sequence of Amycolatopsis sp. CA-230715, Encoding a
            35-Module Type I Polyketide Synthase
  JOURNAL   Microbiol Resour Announc 10 (38), e0080521 (2021)
   PUBMED   34553997
REFERENCE   2  (bases 1 to 10363158)
  AUTHORS   Joergensen,T.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2020) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2019
            Assembly Method :: Flye v. 2.4.1-geb89c9e; Unicycler v. 0.4.8-beta;
            SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology :: Illumina MiSeq; PacBio RSII; Oxford
            Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 09:21:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,592
            CDSs (total)                      :: 9,498
            Genes (coding)                    :: 9,434
            CDSs (with protein)               :: 9,434
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 10 of 64
            Pseudo Genes (incomplete)         :: 60 of 64
            Pseudo Genes (internal stop)      :: 2 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:58:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5224450
            Orig. end                         :: 5234843
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059997.1.
            Annotated using prokka_raw 1.14.6 from
            https://github.com/tseemann/prokka.
            Bacteria and source DNA available from Fundacion MEDINA, Grenada,
            Spain.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="23"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5393](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5393
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(56..70)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.99"
                     /tool="antismash"
     misc_feature    233..246
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.82"
                     /tool="antismash"
     gene            353..700
                     /locus_tag="HUW46_RS25205"
                     /old_locus_tag="HUW46_04994"
     CDS             353..700
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664372.1"
                     /locus_tag="HUW46_RS25205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04994"
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="WP_215541292.1"
                     /transl_table=11
                     /translation="MAHAREGKHAAEETPEAAEVPAARPAADDQHPPKRTRLGGTWVAV
                     VVALVVLVFLLIFILQNLENATVHFLGAVGSMPLAVAMLLAAVAGGALVALVGAARILQ
                     LRAQNRKSRKN"
     gene            799..1593
                     /locus_tag="HUW46_RS25210"
                     /old_locus_tag="HUW46_04995"
     CDS             799..1593
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664374.1"
                     /locus_tag="HUW46_RS25210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04995"
                     /product="Abi-alpha family protein"
                     /protein_id="WP_254124894.1"
                     /transl_table=11
                     /translation="MSNEDRLVGPAAEPGIVQRAGKFAGWAARTGYSLSKRLPGVEAAE
                     KGLRQVERIALGELRRRLDEVDDPYLAALSAASTISPQNGVEGNGKLSDSVAVVPARDS
                     EPLRAAMAELLNRSLGFGKERAREYLYAIILRQLTPDEARILSALSDGSPFPVIDVFER
                     TSLTGTGRTVLRNASTVGKAAGVSLPAETPSYVTRLVGLGLADLDEEVDSLATQYEILL
                     TDETVRAAEDSVKRTKIVRRTVHLSHLGTQFWRACDPAAGPA"
     misc_feature    complement(1625..1640)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.13"
                     /tool="antismash"
     gene            1664..2899
                     /locus_tag="HUW46_RS25215"
                     /old_locus_tag="HUW46_04996"
     CDS             1664..2899
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631931.1"
                     /locus_tag="HUW46_RS25215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04996"
                     /product="DUF445 domain-containing protein"
                     /protein_id="WP_215541293.1"
                     /transl_table=11
                     /translation="MGAILDDFARHWPIYVSIPLVAALIGYVTKLVAIRMMFQPLEFVG
                     VKPVFGWQGIVPKRAARMAGIACDTMTQQLVKPADVIERLDAERVAKEIEKPLLAATEE
                     IVRDVAGQYQSGLWEALPARVQRLVVHRVQAEAPRMVGAVLDAIKHDVDSVFDLKDMVV
                     TSLVKDKALLNRIFQEAGRKEFQFIARSGIVFGGLIGIIQMVAWVLFKIPLIMPLFGLF
                     TGWFTDWLALKMIFHPKQPVRYFGLFEWQGLFLKRRAEVSEAYGELIAKEIITPRNVIE
                     AVLRGPLSDKVLGMIQRQVDEELSRQTSVAKPLVVMAVGSRRYQEMKAVIAEKIMDRLP
                     ETMRYLEDYAEDAMDIRNLLVRKMKELDADEFEGLLRPAFQQDEWILITTGAVLGFAVG
                     ELQVLVLEHFAA"
     gene            3178..3678
                     /gene="ectA"
                     /locus_tag="HUW46_RS25220"
                     /old_locus_tag="HUW46_04997"
     CDS             3178..3678
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664377.1"
                     /locus_tag="HUW46_RS25220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04997"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_215541294.1"
                     /transl_table=11
                     /translation="MSGKHLIESPTKADGAELWRIARDSQKLDLNSPYAYLLWCHDFAD
                     TSVVAKVDGRAVGFVIAYRKPSAPDAALVWQVAVDASQRGQGLAGKLLDELFAKLTGEG
                     VRYLETTITPDNEASIKLFSSFAKRWETDLESAELFSASDFPDDGESHEPEDRYRIGPL
                     VRA"
     misc_feature    complement(3705..3720)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 25.67"
                     /tool="antismash"
     gene            3716..4969
                     /gene="ectB"
                     /locus_tag="HUW46_RS25225"
                     /old_locus_tag="HUW46_04998"
     CDS             3716..4969
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 3.5e-74.
                     Score: 241.8. Matches aSDomain:
                     nrpspksdomains_HUW46_RS25225_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230319.1"
                     /locus_tag="HUW46_RS25225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04998"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_215541295.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.6e-75, bitscore:
                     252.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIFETLESEVRSYSRGWPVVFDRAKGSYLYDEDGREYLDFFAGA
                     GALNYGHNNPALKQALIDYISRDGVTHALDMFTVAKRDFLQTLDDKILKPRDLEYKVVF
                     PGPGGANAVEAALKLARKVTGKESVINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHA
                     TPMPYDKYFDGQFPDFFYFEKLLEDSGSGLNEPAAVIVEGVQGEGGINAARIDWLKGLS
                     DLCERHDILLILDDVQMGCGRTGPFFSFEEAGIKPDIVCLSKSIGGYGLPMAITLIRPD
                     LDVWEPGEHNGTFRGISPAFVTAAEALRVYWSDDELEKSTKAKGERIADAFKGIVEAYP
                     EANLLAKGRGLARGLEFADGDLAGKVCAAAFERGLLMETSGPDGEVMKLLPPLTLSDEE
                     LGKGLDIISESIKTVLHK"
     aSDomain        3779..4789
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HUW46_RS25225_Aminotran_3.1"
                     /evalue="3.50E-74"
                     /label="HUW46_RS25225_Aminotran_3.1"
                     /locus_tag="HUW46_RS25225"
                     /protein_end="358"
                     /protein_start="21"
                     /score="241.8"
                     /tool="antismash"
                     /translation="FDRAKGSYLYDEDGREYLDFFAGAGALNYGHNNPALKQALIDYIS
                     RDGVTHALDMFTVAKRDFLQTLDDKILKPRDLEYKVVFPGPGGANAVEAALKLARKVTG
                     KESVINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHATPMPYDKYFDGQFPDFFYFEK
                     LLEDSGSGLNEPAAVIVEGVQGEGGINAARIDWLKGLSDLCERHDILLILDDVQMGCGR
                     TGPFFSFEEAGIKPDIVCLSKSIGGYGLPMAITLIRPDLDVWEPGEHNGTFRGISPAFV
                     TAAEALRVYWSDDELEKSTKAKGERIADAFKGIVEAYPEANLLAKGRGLARGLEFA"
     gene            5001..5393
                     /locus_tag="HUW46_RS25230"
                     /old_locus_tag="HUW46_04999"
     CDS             5001..5393
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947387.1"
                     /locus_tag="HUW46_RS25230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04999"
                     /product="ectoine synthase"
                     /protein_id="WP_215541296.1"
                     /sec_met_domain="ectoine_synt (E-value: 9.2e-55, bitscore:
                     183.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTLDEITDTDADIKTENWRSKRIVLAKEKVGFSVHETTLYAG
                     TVNDFWYANHIEAVFVTQGEGEIEDKATGEVHQLRPGSLYLLNNHDKHQVRPKTEMKTV
                     CVFNPPVTGREVHDENGVYPLVVEED"
     gene            5481..6383
                     /gene="thpD"
                     /locus_tag="HUW46_RS25235"
                     /old_locus_tag="HUW46_05000"
     CDS             5481..6383
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="HUW46_RS25235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05000"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_215541297.1"
                     /sec_met_domain="PhyH (E-value: 5.6e-32, bitscore: 110.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLMDTRVEDSYPTRITGEPVAFDRPDPTVWGAESDGPIDAATLA
                     SHDARGFHVIEGLLSPAEVQAYWQDLVRLSSDEALKADERVITEKASGEVRSIFDMHKI
                     SSLIAELVRDPRILDRAKQILGSEVYLHQSRVNYMPGFKGAGFYWHSDFETWHAEDGMP
                     KPRAVSCSIALTDNYPFNGGLMVMPGSQRTFVPCVGKTPEENYKSSLKEQEFGVPNRNE
                     VTKLAAECGIEQFTGPAGSALWFDSNIMHGSGNNITPYPRSNIFLVFNSVENTLGDPYG
                     AAAPRPEFIASRDFTPVVR"
     gene            complement(6701..7564)
                     /locus_tag="HUW46_RS25240"
                     /old_locus_tag="HUW46_05001"
     CDS             complement(6701..7564)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669886.1"
                     /locus_tag="HUW46_RS25240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05001"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_215541298.1"
                     /transl_table=11
                     /translation="MSGSPPTSRVVSVVELLAARPDGCSVAEVTASLELNRATVTSVLA
                     ELERTGWARRRADRSYVLGPGLLGVTEAVRAALPLPRGAADRVAALADEVRCGASLSLV
                     EPHRMTFVAVSESVSGRLPGGLAVGTRIPLLPPTGATVMAWRDAREQRAWLDDADEHER
                     EGFREMLAALAGRAITVWRLDDSAADLLGVLADVVDALADHPTRHHLRRRALTELSRLV
                     GNAYRTDELETDRPLPVSYLAAPVFAGGAATHELQIGPLHPALARGERERMALALMAAA
                     GELSQP"
     gene            7683..8855
                     /locus_tag="HUW46_RS25245"
                     /old_locus_tag="HUW46_05002"
     CDS             7683..8855
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669887.1"
                     /locus_tag="HUW46_RS25245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_05002"
                     /product="cytochrome P450"
                     /protein_id="WP_215541299.1"
                     /sec_met_domain="p450 (E-value: 6.1e-20, bitscore: 70.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLGELDVEGVDFASGNIPNAHRLLATLRRRKPYAVVPFGGGRAI
                     VLLTHELVTAAFKDEETFPAAAAHPPSGPVLGHTVQCMAGAAHRIHERLATPAFHDGLV
                     GGYGSALFEPVAHELVDRFSRHEVDLVVEFTEQYPVLVVARMLGLPLADESVLWRWMHD
                     LYYYPVEPDGAARASDEFGAYLREIIRRRREHPGDDLISTLLTVDGYSDESVLAFARLL
                     FPAAADTVMLSLGNILAALLTHPAQLAKVRRHPKTELSWAVQEALRWEPPVGMLPRLCT
                     RGTEWHGITIPPATPVIFAITAANRDPAVYPDPDVYDLDRRAMATLAFGDGAHTCLGAR
                     FALAELTAGLKVLVERLPDMRLAPGADARVGSRMTPVLRGPSSLPVLLGR"
     misc_feature    7764..7766
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(9126..9139)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     misc_feature    complement(9187..9201)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
ORIGIN
        1 ttctccaacg gcgcgaacat cgcggccgcg ttgacggtgc tgcgcccgga cgtgctgacc
       61 gaggcgatcc tgttcgcctc gatgcttccg gtgcccagtc cgccggagca cgacctgtcc
      121 ggttcgaggg tgttcctgtc gaacggcgag caggatccga tggcgccgct ggcgtcgaac
      181 gacgagttca tcgcactgct gcgggcgagg tcggccgagg tgaccagcca ccggcaccac
      241 ggttgccacc agatcaccgc cgacggcgtg gccgcggcga agaactggct gaggtaaccc
      301 gttcgagtga gttgccgagc gggtggcatg gcccgcgctc acaatccggg ccatggcaca
      361 cgctcgggaa ggcaagcacg cggccgagga gacccccgaa gccgcggagg tccccgccgc
      421 gcgtcccgcg gccgacgacc agcacccgcc gaagcggacc aggctgggcg gcacctgggt
      481 cgccgtcgtc gtcgcgctcg tggtgctggt gttcctgctg atcttcatcc tgcagaacct
      541 ggagaacgcg accgtgcact tcctcggcgc ggtgggcagc atgccgctcg ccgtggcgat
      601 gctcctggcc gcggtggcgg gcggcgcgct cgtcgcactc gtcggcgccg ccaggatcct
      661 gcagctccgc gcccagaacc ggaaatcccg gaagaactga cccggcgcgt ttagcgggct
      721 aaacgcgctc gggggccgtc ggtcgcggag ctgtcttact cctcggtagc atctcggcga
      781 gcagcgaggg agcagcgcgt gagcaacgaa gaccgactgg tgggccccgc ggccgagccc
      841 gggatcgtcc agcgtgccgg caagttcgcc gggtgggcgg cccgcaccgg ctactccctg
      901 tccaagcggc ttcccggggt cgaggccgcg gagaagggcc ttcgccaggt ggagcggatc
      961 gcgctcggcg agctgcgccg ccgcctcgac gaggtcgacg acccgtacct cgcggccttg
     1021 agcgcggcga gcacgattag cccgcaaaac ggcgtcgagg gcaacgggaa gctcagcgat
     1081 tcggtcgcgg tcgtgcccgc gcgcgacagc gagccgttgc gcgccgcgat ggccgagctg
     1141 ctcaaccgct cgctcggctt cggcaaggag cgcgcccgcg agtacctcta cgcgatcatc
     1201 ctgcgccagc tcacccccga cgaggcacgc atcctgtccg cgctgtccga cgggtcgccg
     1261 ttcccggtga tcgacgtctt cgaacggacc agcctcaccg gcaccgggcg caccgtgctg
     1321 cgcaacgcgt cgacggtggg gaaggcggcc ggggtttcgc tgcccgcgga gaccccgagc
     1381 tacgtcaccc gcctcgtcgg cctcggcctc gccgacctcg acgaagaggt ggattccctg
     1441 gccacgcagt acgagatcct gctgaccgac gagacggtcc gcgccgcgga ggactcggtg
     1501 aagcggacga agatcgtgcg gcggaccgtg cacctgtcgc acctcggcac ccagttctgg
     1561 cgcgcgtgcg atccggccgc gggccccgct tgatcgatct gcggtcgcgc cgatcacccg
     1621 atcatgtgac catggtcacc cgggctagtg aaacggggtg gtcatgggtg cgattctcga
     1681 cgacttcgca cgccactggc cgatttacgt ctccatcccg ctcgtcgcgg cgctgatcgg
     1741 gtacgtgacc aagctggtcg cgatccggat gatgttccag ccgctcgagt tcgtcggcgt
     1801 caaacccgtc ttcggctggc agggcatcgt gcccaagcgc gcggcgcgga tggcgggcat
     1861 cgcgtgcgac accatgaccc agcagctggt gaaaccagcc gacgtcatcg aacgcctcga
     1921 cgccgagcgc gtcgcgaagg agatcgaaaa acccctgctc gccgcgaccg aggagatcgt
     1981 ccgcgacgtc gcggggcagt accagtccgg gttgtgggag gcgctgcccg cgcgggtcca
     2041 gcggctggtc gtgcaccgcg tgcaggccga ggcgccgagg atggtcggcg cggtgctcga
     2101 cgcgatcaag cacgacgtcg acagcgtctt cgacctcaag gacatggtcg tgacgagcct
     2161 ggtcaaggac aaggcgctgc tcaaccggat cttccaggag gcgggccgca aggagttcca
     2221 gttcatcgcc cgctccggca tcgtcttcgg cgggctgatc ggcatcatcc agatggtggc
     2281 ctgggtgctg ttcaagatcc cgctcatcat gccgctgttc ggcctgttca ccggctggtt
     2341 caccgactgg ctcgcgctga agatgatctt ccacccgaag cagccggtgc ggtacttcgg
     2401 cctgttcgaa tggcagggcc tgttcctcaa gcggcgcgcg gaggtgtccg aagcgtacgg
     2461 agagctgatc gcgaaggaga tcatcacgcc gcgcaacgtg atcgaagcgg tgttgcgcgg
     2521 gccgctgtcg gacaaggtgc tcggcatgat ccagcgccag gtcgacgagg aactgagccg
     2581 ccagacgagc gtggcgaaac ccctggtcgt gatggccgtc ggcagcaggc gctaccagga
     2641 gatgaaggcg gtcatcgcgg aaaagatcat ggaccggctc ccggagacca tgcggtacct
     2701 cgaggactac gccgaggacg cgatggacat caggaacctg ctggtgcgga agatgaagga
     2761 gctcgacgcc gacgagttcg agggcctgct gcggccggcc ttccagcagg acgagtggat
     2821 cctgatcacc accggcgcgg tgctgggctt cgccgtcggt gagctgcagg tgctcgtcct
     2881 ggagcacttc gcggcctgaa cgcggatgac gcaggggctt caggtaacgc cgtgcgagcg
     2941 gacggcgaca ttactcccgg cgtcgtctcg caattcgtga gtgctgccac taccgtatgg
     3001 aacggtagcc gaccgtgacg ggcggcggcg aagcctgggc atcttacccg gcgcgctgac
     3061 cgattggtac gttactgtcg gcattgtcac aggggtttct ccgtccctgc aggtagcccg
     3121 cctgctcttc cggcgctgtt ctgaaccggc agaaggcttc tgttaggttt cgtagacatg
     3181 tccggaaagc acttgatcga atccccgacc aaggcagatg gcgcggagct gtggcgaatc
     3241 gcacgcgatt cccagaagct cgatctcaac tcgccctatg catatctgtt gtggtgccac
     3301 gatttcgccg acacttcggt ggtcgcgaaa gtcgacggcc gagcggtcgg cttcgtgatc
     3361 gcctatcgca agccgtccgc acccgacgcc gcgctcgtgt ggcaggtggc cgtcgatgcc
     3421 tcccagcgtg gtcagggcct ggcagggaag ctgctcgacg agctcttcgc caagctcacc
     3481 ggcgagggcg tgcgctacct ggagaccacc atcaccccgg acaacgaggc ctccatcaag
     3541 ttgttcagct cgttcgccaa acggtgggaa accgacctgg aatcggcgga actgttcagc
     3601 gcgagcgatt ttccggacga tggcgagtcg catgagccgg aggaccgata ccgcatcggc
     3661 ccgcttgtgc gcgcatagga aatatcaaga cttcccaggg gcaggagaaa aaaccgtgaa
     3721 catcttcgag accctcgaat ccgaggttcg cagttacagc aggggctggc cggtcgtgtt
     3781 cgaccgcgcg aagggcagtt acctctacga cgaggacggc cgggaatacc tcgacttctt
     3841 cgccggcgcg ggcgcgctga actacgggca caacaacccg gcgctgaagc aggcgctcat
     3901 cgactacatc tcgcgagacg gcgtcacgca cgcgctggac atgttcaccg tggccaagcg
     3961 cgacttcctg cagaccctcg acgacaagat cctcaagccg cgcgatctcg agtacaaggt
     4021 cgtgttcccc ggccccggcg gcgccaacgc ggtcgaggcc gcgctcaagc tggcgaggaa
     4081 ggtgaccggc aaggagtcgg tcatcaactt cacgaacgcc ttccacggga tgacgctcgg
     4141 cgcgctctcg gtgaccggca actcgatgaa gcgcggcggc gcgggcatcc cgctcgtgca
     4201 cgccaccccg atgccgtacg acaagtactt cgacggccag ttcccggact tcttctactt
     4261 cgagaagctg ctggaggact cgggcagcgg gctgaacgag cccgccgcgg tgatcgtcga
     4321 aggcgtgcag ggcgaaggcg gcatcaacgc cgcccgcatc gactggctca agggcctgtc
     4381 ggacctgtgc gagcggcacg acatcctgct gatcctggac gacgtgcaga tgggctgcgg
     4441 ccgcaccggg ccgttcttca gcttcgagga agccgggatc aagcccgaca tcgtgtgcct
     4501 gtcgaagtcg atcggcgggt acggcctgcc gatggcgatc acgctcatcc ggcccgatct
     4561 cgacgtgtgg gagccgggcg agcacaacgg cacgttccgc ggcatcagcc cggcgttcgt
     4621 gaccgccgcc gaggcgctgc gcgtgtactg gagcgacgac gagctcgaga agtcgacgaa
     4681 ggcgaagggc gagcgcatcg ccgacgcgtt caagggcatc gtcgaggcct acccggaggc
     4741 gaacctgctc gcgaagggac ggggtcttgc gcgcgggctc gagttcgccg atggtgatct
     4801 tgccgggaag gtctgcgccg ccgccttcga acgcgggctg ctgatggaga cctccggtcc
     4861 cgacggagag gtgatgaagc tgctgccgcc gctgaccctg agcgacgagg agctgggcaa
     4921 gggcctcgac atcatctccg agtcgatcaa gaccgtgctg cacaagtagc acctgaaaaa
     4981 cgcgaaagag gagtaccgag ttgatcgtcc gcacactcga cgagatcacc gacaccgacg
     5041 ctgacatcaa gaccgaaaac tggcgctcga agcgcattgt tctcgccaag gaaaaagtcg
     5101 gtttctcggt gcacgagacg accctctacg cgggcacggt caacgatttc tggtacgcga
     5161 accacattga agcggtgttt gtgacgcagg gcgaagggga aatcgaggac aaggcgaccg
     5221 gcgaggtgca ccagctgcgt cccggttccc tgtacctgct gaacaaccac gacaagcacc
     5281 aggtgcgccc gaagaccgag atgaagacgg tgtgcgtctt caacccgccg gtgaccggca
     5341 gggaggtgca cgacgaaaac ggcgtgtacc cgctcgtggt cgaagaggac tgagccaccc
     5401 ggaactgact gccgacacca cggtatcccg ccgacgaatg gggcgaaaaa aaccgaagga
     5461 aagctgagga ggcgaaaatc ttgacgctca tggacacgcg cgtcgaggac agttatccga
     5521 cccggatcac cggtgagccg gtggcattcg accggccgga tccgacggtg tggggggccg
     5581 agtccgacgg ccccatcgac gccgccaccc tcgcgtcgca cgacgcgagg ggtttccacg
     5641 tcatcgaggg cctgctgtcc cctgccgagg tgcaggcgta ctggcaggac ctcgtgcggc
     5701 tgtcttcgga cgaggcgctg aaggccgacg aacgggtgat aaccgagaag gcgtcgggcg
     5761 aggtgcggtc cattttcgac atgcacaaga tcagctcgct gatcgcggaa ctggtgcgcg
     5821 acccgaggat cctcgatcgg gcgaagcaga tcctcggctc cgaggtctac ctccaccaga
     5881 gccgggtcaa ctacatgccg ggcttcaagg gtgccgggtt ctactggcac tccgacttcg
     5941 aaacctggca cgcggaggac ggcatgccga agccgagggc ggtgagctgc tcgatcgcgc
     6001 tgaccgacaa ctacccgttc aacggcgggc tgatggtgat gcccggttcc cagcggacct
     6061 tcgtgccgtg cgtcggcaag accccggagg agaactacaa gagctccctc aaggagcagg
     6121 agttcggcgt gccgaaccgg aacgaggtga cgaagctggc cgcggagtgc ggtatcgagc
     6181 agttcaccgg tccggcgggt tccgcgctct ggttcgactc gaacatcatg cacgggtccg
     6241 gcaacaacat cacgccgtac ccgaggtcga acatcttcct ggtgttcaac agtgtcgaga
     6301 acacgctcgg cgacccgtac ggcgcggccg cgccacgccc ggagttcatc gcctcccgtg
     6361 acttcacgcc ggtggtgcgc tgacttctcc gacatgcggc gggggcctcg gggcacgggg
     6421 ctcccgccgt aattttttcg ccagggtggt tgtgatcgac cgggcgcgtg ctgttacctt
     6481 gtcggcaccg cttcggaggt acgaggtgct ggagtggtga gatgttgccg gtgtgacggt
     6541 gtgtccctct tgctcggggt gagcccaccg aaggactggt agcgatgtcc ggcgctggag
     6601 cttcgggttc tccggtgccg cgtcgccgca ccgagtacgg ccccgcacca tcagatcggg
     6661 actgatgatg cggggccgta ctcgttccac ggcaagggtt tcagggctgg gacagctctc
     6721 cggccgcggc catcagcgcc agcgccatcc gctcgcgctc gccgcgcgcc agcgccgggt
     6781 gcagcggccc gatctgcagt tcgtgcgtcg cggccccgcc cgcgaacacc ggcgccgcga
     6841 gatagctgac cggcagcggg cgatcggttt ccagttcgtc cgtgcggtag gcgttgccca
     6901 ccagcctgct cagttcggtc agtgcgcggc ggcggaggtg gtggcgcgtc ggatgatccg
     6961 cgagcgcgtc gaccacgtcc gcgagaacgc cgagcagatc ggccgcgctg tcgtcgagcc
     7021 gccacaccgt gatcgcgcgc ccggccagcg ccgcgagcat ctcgcggaac ccttcgcgct
     7081 cgtgttcgtc ggcgtcgtcg agccatgccc gctgctcgcg cgcgtcgcgc caggccatca
     7141 ccgtggcacc cgtcggcggc agcagcggga tccgcgtgcc gaccgcgagg ccgccgggga
     7201 gcctgccgga gacgctttcg gacaccgcga cgaaggtcat ccggtgcggc tcgacgaggc
     7261 tcagcgaggc accgcaccgc acttcgtcgg cgagcgcggc gacccggtcc gcggcgccgc
     7321 gtggcagggg gagcgcggcg cggacggcct cggtgacgcc gagcaaaccc ggccccagca
     7381 cgtacgagcg gtccgcgcgg cggcgtgccc acccggtgcg ttcgagttcg gcgagcaccg
     7441 aggtgacggt ggcgcggttc agttcgaggc tcgccgtcac ctcggcgacc gagcagccgt
     7501 cggggcgggc ggcgagcagt tcgacgaccg acacgacgcg cgacgtcggc ggtgaaccac
     7561 tcacagctcg aacctcttgt gcttcccgcc cgcctgcgca tagtgtcgcg ggcatcgaat
     7621 aatcgtaaga atagcgacga atattcgtca tcaccagtga tcgtcttgga gcaggggccg
     7681 gcatgacgct gggagagttg gatgtcgaag gagtcgattt cgcgtccggg aacatcccga
     7741 acgcccatcg cctgcttgcc acgttacgcc ggcgcaagcc ctacgcggtg gtcccgttcg
     7801 gcggtggccg cgcgatcgtc ctgctgacgc acgagctggt gaccgccgcg ttcaaggacg
     7861 aggaaacgtt ccccgccgcc gcggcgcacc cgccgtcggg gccggtgctc ggccacaccg
     7921 tgcagtgcat ggccggtgcg gcgcaccgga tccacgagcg gctggccacc cccgccttcc
     7981 acgacgggct ggtcggcggg tacgggagcg cgctgttcga acccgtcgcg cacgagctcg
     8041 tcgaccggtt ctcccggcac gaggtcgatc tcgtcgtcga gttcaccgag cagtacccgg
     8101 tgctggtcgt ggccaggatg ctgggcctgc cgctcgccga cgaatcggtc ctctggcgct
     8161 ggatgcacga tttgtactac tacccggtcg aacccgacgg cgcggcgcgc gcgtccgacg
     8221 agttcggcgc ctacctgcgc gagatcatcc ggcgccgccg cgaacatccc ggcgacgacc
     8281 tgatttcgac gctgctcacc gtggacgggt acagcgacga atccgtgctc gcgttcgcgc
     8341 gcctgctgtt ccccgccgcg gccgacaccg tgatgctgtc gctcggcaac attcttgccg
     8401 cgctgctgac ccacccggcc cagctcgcga aggtgcgccg ccaccccaag accgagctgt
     8461 cctgggcggt gcaggaagcg ctgcggtggg agccgccggt cggcatgctg ccgaggctct
     8521 gcacgcgcgg caccgaatgg cacggcatca cgattccgcc tgccacaccg gtgatcttcg
     8581 cgatcaccgc ggccaaccgc gaccccgcgg tctaccccga tccggacgtc tacgacctcg
     8641 accggcgcgc gatggcgacg ctcgccttcg gcgacggcgc gcacacctgc ctcggcgcgc
     8701 ggttcgcgct cgcggagctg accgccgggc tgaaggtgct cgtcgaacgg ctgcccgaca
     8761 tgcggctcgc accgggcgcg gacgcacggg tcggcagcag gatgaccccg gtcctccgcg
     8821 gcccctccag cctcccggtc ctcctcggcc gctagagact gtttcgaagt gctttgcgtg
     8881 gcggtgccgg tagcggaacc tcaggcggcc cctcactccg ggatctcccc ctcatgaccg
     8941 ccaggtcacc acggtggagc tgtcctcgcg aggaaccacc tgagaacccg cgccggtgca
     9001 aggtgacggc ccactcaagt ggctccgcca cttcgataca ggcgctagcg aaccgtttcg
     9061 acgtatagcg gcacacgaat cggtgccgcc cagatcctcc acttcgggcc gactcccggg
     9121 taggttggat cccggtcgaa gtgcagttag cgggctaaac gcgctcgtgg aaccgggagg
     9181 gatggttggc cgaggcagac ctgcgtttcc gggttctcgg tcccgtccag ctggtggcag
     9241 gcgggcagcc ggtgccgatc ggcgggcccg gcgtgcgggg cctgctcgcg atgctggcgc
     9301 tcgaggcgaa ccggatcgtc gcgctggacc gcatcatcga cgcgctgtgg gaccacgatc
     9361 cgccggccac cgcgcggacc atcgtgcacg gcaacgtttc cgcgctgcgc cgcacgctcc
     9421 gttcggtgca gggccccgac ggcgagatca ggatcgacac cgcgacaccg ggctaccggc
     9481 tcgtcgccag cccggagcgc atcgacgtgt accgggcccg caggctgctc gaagccgcgc
     9541 ggggccgcgc gccgggcgag agggtcgcgc tgctctccga agccagcgcg ctgtgggaag
     9601 gtccgtcgct cgtgggcgtc ccggacggac tgcgcgcgcc ggagctggcc gacctgcgcc
     9661 tcgcggtgcg cggcgagctg atggacgccc agctggcgct cggcaagtac accgacgtga
     9721 tcaccgaact cagccaggtg gtcagggacg atcccctcgc cgagcgctcg gtcggccagc
     9781 tgatgcgcgc gctgtacttc accgggcgcc gggcggacgc gctgcaggcc taccggcgca
     9841 cggcgcgttt cgcggtcgaa acgctgggca tcgggcccgg ccccgaactg cacgatctgc
     9901 acgaccggat cctcaacgac gagctggtgc cgtccaccga gctcagcacc cggcgcgcgg
     9961 cgccgagggc gggcgctccg gcgcagctcc cgtcgacggt cccggtgctg gtcgggcgga
    10021 cggccgagct gggctggctc gacgagcagc tcgcgcgctc cggcgagggc ggtggcgcgg
    10081 tcgccgtcgt caccgggacg gcggggatcg gcaagagcgc gctcgtggtg tcgtgggcgc
    10141 accgcgtctc gccgcgcttt cccgacggcg tgctgttcgc ggcgctgcgc gggttttcct
    10201 cggaggactc cccgctccgg ccgcacgcgg cgctcgcgca gttcctccgc gggctcggtg
    10261 tcgcgcccgc cgagataccc gagccgatcg aggagcggat cgcgctctac cgctcgctgc
    10321 tcgctgagcg caccgtgctc gtcgtgctcg acgacgcacg cgacaccgaa caggtgcgcc
    10381 cgctgctgcc ccc
//
