LOCUS       NZ_CP059997            22580 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Amycolatopsis sp. CA-230715 chromosome, complete genome.
ACCESSION   NZ_CP059997
VERSION     NZ_CP059997.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. CA-230715
  ORGANISM  Amycolatopsis sp. CA-230715
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 10363158)
  AUTHORS   Jorgensen,T.S., Gren,T., Oves-Costales,D., Ortiz-Lopez,F.J.,
            Carretero-Molina,D., Gonzalez,I., Blin,K., Genilloud,O. and Weber,T.
  TITLE     Complete Genome Sequence of Amycolatopsis sp. CA-230715, Encoding a
            35-Module Type I Polyketide Synthase
  JOURNAL   Microbiol Resour Announc 10 (38), e0080521 (2021)
   PUBMED   34553997
REFERENCE   2  (bases 1 to 10363158)
  AUTHORS   Joergensen,T.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2020) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2019
            Assembly Method :: Flye v. 2.4.1-geb89c9e; Unicycler v. 0.4.8-beta;
            SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology :: Illumina MiSeq; PacBio RSII; Oxford
            Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 09:21:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,592
            CDSs (total)                      :: 9,498
            Genes (coding)                    :: 9,434
            CDSs (with protein)               :: 9,434
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 10 of 64
            Pseudo Genes (incomplete)         :: 60 of 64
            Pseudo Genes (internal stop)      :: 2 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:58:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4880914
            Orig. end                         :: 4903494
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059997.1.
            Annotated using prokka_raw 1.14.6 from
            https://github.com/tseemann/prokka.
            Bacteria and source DNA available from Fundacion MEDINA, Grenada,
            Spain.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22580
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lanthipeptide-class-iii"
                     /region_number="21"
                     /rules="(micKC and not PKS_KS)"
                     /tool="antismash"
     cand_cluster    1..22580
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(micKC and not PKS_KS)"
                     /kind="single"
                     /product="lanthipeptide-class-iii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22580
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12580](+)"
                     /cutoff="20000"
                     /detection_rule="(micKC and not PKS_KS)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-iii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12580
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(micKC and not PKS_KS)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-iii"
                     /protocluster_number="1"
     misc_feature    414..428
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.39"
                     /tool="antismash"
     gene            643..1800
                     /locus_tag="HUW46_RS23455"
                     /old_locus_tag="HUW46_04642"
     CDS             643..1800
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="resistance (resist) Chlor_Efflux_Pump"
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237079.1"
                     /locus_tag="HUW46_RS23455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04642"
                     /product="Cmx/CmrA family chloramphenicol efflux MFS
                     transporter"
                     /protein_id="WP_215549320.1"
                     /transl_table=11
                     /translation="MPFAVYMLGLAVFTQGTSEFMLSGLVADIARDLHVSIPAAGLLTS
                     AFAVGMVIGAPLMAVLGLRWSRRRALTGFLVVFIAVHVVGALTPGYEVLLCARVVGALA
                     NAGFWATALATANALVEPGLKARATSTVVGGVTLSCVVGVPAGALLGQYWGWRAAFWAV
                     AVLSVPAVFAILRAIPGGRPPSASGTTAREELRVLRNPRVLVVLLLGALVNGATFCTFT
                     YLTPLLTGVTGFGEGWMPALLALFGAGSFVGVAVGGRIADARPLALLWAGSAALCAGWV
                     VFALAAGVPVLAVVFVLVQGILSFGVGSTLVARVFSVATGAPTLAGALATAAFNVGATA
                     GPWLGGLAIGAGGGFRSPVWVSAALVALAFAVFALSRVPTRSRTR"
     gene            1923..2804
                     /locus_tag="HUW46_RS23460"
                     /old_locus_tag="HUW46_04643"
     CDS             1923..2804
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1260: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /locus_tag="HUW46_RS23460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04643"
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_215549321.1"
                     /transl_table=11
                     /translation="MWRTTGLARGDGFALDELRIVERAPSWSEPVPSAGHRLVFVRRGL
                     FRLRLPRWTGLVDPGTGYASGPGDEQAIAHRPGREDVCTVVTVAESMLPDGWAPCAPVP
                     VAARVDFAHRVLVARTRAGADEFELTERVVRLAGAVTWRAAPKSSPAHRRLAEAAREAL
                     LEDPARATIGELAATLAVSRSHLSRVFHEETGQTLTRFRAGVRVRAALDRLTEGEADLA
                     GLAADLGFADHAHLTRTLRDHLGLPPSKARELLSTNVQAVAAETGHARGDNRGGRDDRT
                     TVAGTGGKQRES"
     gene            2755..3069
                     /locus_tag="HUW46_RS23465"
                     /old_locus_tag="HUW46_04644"
     CDS             2755..3069
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HUW46_RS23465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HUW46_04644"
                     /product="hypothetical protein"
                     /protein_id="WP_215549322.1"
                     /transl_table=11
                     /translation="MIGRLWQARAENSAKADAYEEVFRGAVLAELRDLRGFRGAYLLRR
                     DTGFLTMTLFDGLDDVRGFTGRDVETANVSGAARAVLADFDTTARHFRVVVDPSFSDGH
                     "
     gene            complement(3079..3258)
                     /locus_tag="HUW46_RS23470"
                     /old_locus_tag="HUW46_04645"
     CDS             complement(3079..3258)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HUW46_RS23470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HUW46_04645"
                     /product="hypothetical protein"
                     /protein_id="WP_215549323.1"
                     /transl_table=11
                     /translation="MEKPPVSGWTLAFYAALPLAGLFVAYTGLLRPNLVQVAMGLCGTA
                     FGARMFIVLRRRLR"
     misc_feature    complement(3335..3349)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.16"
                     /tool="antismash"
     gene            3348..4157
                     /locus_tag="HUW46_RS23475"
                     /old_locus_tag="HUW46_04646"
     CDS             3348..4157
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /NRPS_PKS="Domain: Polyketide_cyc2 (131-257). E-value:
                     1.1e-09. Score: 30.8. Matches aSDomain:
                     nrpspksdomains_HUW46_RS23475_Polyketide_cyc2.1"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1058: ArsR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082337.1"
                     /locus_tag="HUW46_RS23475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04646"
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_254126635.1"
                     /transl_table=11
                     /translation="MGEVLNEVFKALADETRRYLLDRLHERNGQTLGELCEGVGMTRQS
                     ATQHLQVLEAANLISVVRRGREKLHYLNPVPLHEIQERWIDKYERPRLRALSTLKNRAE
                     NLMTKPSFVYVPTRHPATTPQGIASRYVIYIESTPEKVWQALTDADLTAEYWGHRNVSD
                     WQPGSAWEHQRTDGSGIADVAGTIVESTEPAKLVITWSLPGTDGEPSQVTFDIEPHGEI
                     VRLTVTHENLADEAERESAAKGWAAVLSNLKSLLETGHVLPRAPWEM"
     aSDomain        3741..4118
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HUW46_RS23475_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="1.10E-09"
                     /label="HUW46_RS23475_Polyketide_cyc2.1"
                     /locus_tag="HUW46_RS23475"
                     /protein_end="257"
                     /protein_start="131"
                     /score="30.8"
                     /tool="antismash"
                     /translation="VIYIESTPEKVWQALTDADLTAEYWGHRNVSDWQPGSAWEHQRTD
                     GSGIADVAGTIVESTEPAKLVITWSLPGTDGEPSQVTFDIEPHGEIVRLTVTHENLADE
                     AERESAAKGWAAVLSNLKSLLE"
     gene            4237..5337
                     /locus_tag="HUW46_RS23480"
                     /old_locus_tag="HUW46_04647"
     CDS             4237..5337
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1175: pyridine nucleotide-disulfide oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804652.1"
                     /locus_tag="HUW46_RS23480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04647"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_215549325.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.7e-42, bitscore:
                     144.8, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAEKIVVVGAGYTGLAAAKLAAKWTGAEVTLVNASDRFVERVRL
                     HQLASGQRLRDLPLRDLLAGTGARLVVGRVRAIDPDAREVLLDDGAPIGYDKLIYALGS
                     HANLDGVPGAAEHAYTVASRADAERLRAKLSGSRTVAVVGGGLTGVEAAAELAEAHPGL
                     KVSLVTGGPLGAALSERGTRHLHRVFGRLGVDVRENAKVIEVRADGLVLADGDTVEADT
                     VVWTAGFVVSSIAAEAGFDVDAAGRMVVDEALRSVSHPDVIAIGDAAAIRLANGLELRM
                     ACATGLPTTQHAVRVLAGKATGSPRFRYFNQCISLGRRDALIQFVRADDSPVDAVLKGR
                     IAAFYKEAVVRGTILFERHPVLPTSP"
     gene            complement(5674..6888)
                     /locus_tag="HUW46_RS23485"
                     /old_locus_tag="HUW46_04649"
     CDS             complement(5674..6888)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019816377.1"
                     /locus_tag="HUW46_RS23485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04649"
                     /product="multidrug effflux MFS transporter"
                     /protein_id="WP_215549326.1"
                     /transl_table=11
                     /translation="MSVTGVSTYRPTAGRVAILGGLSACAPMSVDMYLPSMPQIAADLH
                     TGASQVQLTLTTSILGLAFGQLVLGSLSDAFGRRRPLLAGLAVYTVLSLVCLIAPNATV
                     LIALRFAQGFAGAAGPVLARAIVRDLTSGAAMARLVSMLMLVTSLAPILAPALGAQLLA
                     FGSWRGVFVALAVFGAVLLVACALWLPETLPSEARHTGGLSTSLRAYRTLLADRTFLGY
                     ALTSGLVFAGYFGYLSGSAFVLEQGHGLSTQEFSVVFGANAIGLLAAGQSNAWLVRRYP
                     PRRMLIIGVVAGAVAVVALTAAALAGLGLPALLPPMFVAVAAMGLVAPNCTALGMADHP
                     SIAGSASALLGVLQFVAGGIAGPLVGLGSAGGASDLSMALVMLVGGVGALLFIPRRGSR
                     STPRK"
     misc_feature    6862..6877
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.23"
                     /tool="antismash"
     gene            6945..7592
                     /locus_tag="HUW46_RS23490"
                     /old_locus_tag="HUW46_04650"
     CDS             6945..7592
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /locus_tag="HUW46_RS23490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04650"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_215549327.1"
                     /transl_table=11
                     /translation="MSPEPQTRARRSDAYRNDERILVAAREVFAELGADAPVSAIADRA
                     GVGMSALYRRYPSKDDLMRAIMRDTMERTEREALRALDEPDAWAGFLGFVHACADAGIT
                     GAPRISGAVRPTEEMLARSKRAREAVQALVERTQAEGGLRADVNAHDIVFLLGELRSQR
                     AAKVIAPPGVQERLLGVVLDGLRAPNATPLTARPGTWEDMHEAWRAAQREHG"
     gene            7648..7998
                     /locus_tag="HUW46_RS23495"
                     /old_locus_tag="HUW46_04651"
     CDS             7648..7998
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156756.1"
                     /locus_tag="HUW46_RS23495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04651"
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="WP_215549328.1"
                     /transl_table=11
                     /translation="MGRLGELERAVMDALWDRGEPATVRVVHADLAERDLAYTTVMTVL
                     TRLADKGLVRRERDGRAWRYVPAAKREDYIAELMLEALALTGDRGPALVRFANSVSTDE
                     AEALRRALENPR"
     gene            7995..8846
                     /locus_tag="HUW46_RS23500"
                     /old_locus_tag="HUW46_04652"
     CDS             7995..8846
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005442002.1"
                     /locus_tag="HUW46_RS23500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04652"
                     /product="M56 family metallopeptidase"
                     /protein_id="WP_215549329.1"
                     /transl_table=11
                     /translation="MIYAFHHFVTFGMAVSAMWWLPRGTWTHASPRTALVLWQLVLCAG
                     VVSAIGLLFELGLEPYHLGVLPGLARVVDEGHVPNPVILAATALTVWLVVSLIRLVAST
                     ARVRARHRGLLALVARTDGDALVLDHSAAVAYCLPGRQPRIVISAGARELLTDDELRAV
                     LAHERAHLRQRHHLVLEPFQAWRLFVPRRALSAIGLLVEMCADESAARRHGRAVLASAL
                     EKFGTSAAPAGGLAAGSVGTLARIERLRHPRPAAALGVRLLAVATALAALLTPLTLFVW
                     PS"
     gene            complement(8888..9289)
                     /locus_tag="HUW46_RS23505"
                     /old_locus_tag="HUW46_04653"
     CDS             complement(8888..9289)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663263.1"
                     /locus_tag="HUW46_RS23505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04653"
                     /product="hypothetical protein"
                     /protein_id="WP_215549330.1"
                     /transl_table=11
                     /translation="MARVRDEKKTATGPGRILVAVYAIFALAATSRAAVQLSTKYHEAP
                     LAYLLSAFAAVVYLVATVALARSGRTSWLVALIACGVEFVGVLTVGTLSVFDSAAFPHA
                     TVWSGYGQGYLFIPLVLPVIGLYWLRRTR"
     gene            complement(9273..9638)
                     /locus_tag="HUW46_RS23510"
                     /old_locus_tag="HUW46_04654"
     CDS             complement(9273..9638)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633043.1"
                     /locus_tag="HUW46_RS23510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04654"
                     /product="hypothetical protein"
                     /protein_id="WP_215549331.1"
                     /transl_table=11
                     /translation="MITGFGVTVAACAAAVAVWSFVLAARGREPRRPLLYALGFVELLL
                     VAQLVIGVVLLVGGDRPGSMATYLAYLIGSLVVLPVGTVWALAERSRSSTVVLGIACVA
                     IPVMVLRLSEVWHGASA"
     misc_feature    complement(9820..9835)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.61"
                     /tool="antismash"
     misc_feature    9827..9841
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.98"
                     /tool="antismash"
     gene            10001..12580
                     /gene="lanKC"
                     /locus_tag="HUW46_RS23515"
                     /old_locus_tag="HUW46_04655"
     CDS             10001..12580
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="lanKC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-iii: micKC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LANC_like"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222285.1"
                     /locus_tag="HUW46_RS23515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04655"
                     /product="class III lanthionine synthetase LanKC"
                     /protein_id="WP_215549332.1"
                     /sec_met_domain="micKC (E-value: 0.0, bitscore: 1192.8,
                     seeds: 18, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 4.8e-06, bitscore:
                     24.7, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLRYEAFCFADPLFFDEQRESGAPDDDFSRALPVPDGWVASDRG
                     IWRSLHRLGRQLPLQGWKIHVSANLGNADEVLASVYRYCVRDDYSFKHLRSRSILLARN
                     SKYAPREASGKLVTIYPADNKELERVLVDLSSVLAGQSGPYILSDLRYGDGPLYVRYGG
                     FAEQWVEADGARVLAITKPDGTLVPDERGPRFVVPDWEPLPECLVPHLAARKGGDPRQF
                     PYRVTSSLHFSNGGGVYLADRLADGKRLVLKEARPHAGLDRAGVDAVARLRREHDVLDR
                     LRGITGIPEVYDLFPVWEHHFMAMEQVSGLSLGSWLARNYPLTRRDAEHTDVSGYRARA
                     MALVAEVERMVAEVHERGIVFGDLHPLNVLVETTEATETTGEVDAVSIIDFEMAFDADS
                     GDRPALGAPGFRAPADRSGFEIDAYAVAALKLWIFLPLNTILDFEPGKLHSLVDFAAER
                     FDLPKGYVDEIRQGLAPRSGGSTATATALDDPVPDWQLVRKNIGQAILASATPERQDRL
                     FPGDIEQFRVGGACFAYGAAGVLHALDAAGEGRYPEHERWLLDHVRREPPARPGFYDGA
                     HGIAYVLENFGYAREADALLDGAATMVEQTKEHSLDTGLAGIGLNFLHFAGTRGDTEHT
                     KRALAIGKRLAEALPSAEPPGKFGRAGLLSGWSGPALLFVRLYECTGEESWLALADHAL
                     RRDLDECVAADDESLQVRDGEARTLPYVGIGSAGVALVAEELGEYRPDADSVAALPRLL
                     QASRGEFVIHPGLMFGRAGLMTALAARRDRDEATERAVRLHLSRFGWYAVAYSDGIAFP
                     GNQLLRLSMDLNTGGAGVLLALAATLDGAPALPFLGGSHRPTNPGRS"
     gene            12616..12753
                     /locus_tag="HUW46_RS23520"
                     /old_locus_tag="HUW46_04656"
     CDS             12616..12753
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949109.1"
                     /locus_tag="HUW46_RS23520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04656"
                     /product="SapB/AmfS family lanthipeptide"
                     /protein_id="WP_215549333.1"
                     /transl_table=11
                     /translation="MDMVLDLQAMEAPESVDGGHGGGHGSNLSLLASCANSTISLLTCH
                     "
     gene            13054..14721
                     /locus_tag="HUW46_RS23525"
                     /old_locus_tag="HUW46_04657"
     CDS             13054..14721
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642934.1"
                     /locus_tag="HUW46_RS23525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04657"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_215549334.1"
                     /transl_table=11
                     /translation="MRAPGDRLLSEVTSLERARMPFIVLSALLAAGSGLLLPSALATAV
                     DASIAGSRSWPAILWLLALGAVQIGADVAGGILTVRVTSSATAWLRRTVARHFLALGTK
                     SGFVHGDAISRLTGDAVAAGTVVSILVQLASASVISCGAITLLAVLDWRLALVFAGSVP
                     LALLLARSHLRLTADDVLTYQQVSGEISARLLDATAGLRTIAASGTADREAARVLEPLP
                     RLGVAGGGMWRTQARMVWRAALLLPAVEVTVLVAAGFGVLGGRLTVGDLLAALGYVALG
                     MTMVNQIPLLTTLSRARSGATRLAEVLDSPVPHQGTRPLPPGPGTIELRDVGLRGALEH
                     VGLTVLAGTTLAVVGRSGAGKSALAGVLGGLVEPDEGEVLLDGVPIASVRPEELRAAFG
                     YAFERPSLLGETVSDAVGYGAWSGPSAVRAACRTAQVHDLVVRLPDGYRTPLTETPLSG
                     GEAQRVGLARALARGPRVLVLDDATASLDTVTESLVDKSISDTMPGTTRIVVTHRVAVA
                     RRAGMVAWLDHGRLRAVGPHDTLWAESGYRAIFTDEEP"
     gene            14718..16445
                     /locus_tag="HUW46_RS23530"
                     /old_locus_tag="HUW46_04658"
     CDS             14718..16445
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312163.1"
                     /locus_tag="HUW46_RS23530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04658"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_215549335.1"
                     /transl_table=11
                     /translation="MSIQRLYGAALVGRWRAAAALLACSILEGAPAFFSGRFVELSVDK
                     GFGVGSAGIGVAWLLVFAGVAVLGALGSRLVWHRLGTIVEPIRDALVRAVVRGVLHDAN
                     PPRNQPDASGVARITQHVEVVRDATAGLLVQARGMLVTTAAALAGLFTVAGSLAWLVVV
                     PVVLAITMFGFLLPALATRQRAVALADEALAETAGSALAGMRDVVACGGEAIAGMALYE
                     RVGAQASAAMRLARASALRTLTIAVGGFVPLLLVLAAAPGMVAAGSMTAGAALGALVYL
                     ANTLQPALHGLAATASTVVLRLLVALRRLDETSDVPTPRQGGAEPANSTITVRDLTFGW
                     GAHAEPVVRGLDLDLSPGEYLAVVGPSGIGKSTLAGLLTGLLSPQGGRVLIGGVPVGVV
                     RPAALHRMVALSPQESYVFAGTVRENLALFGTGLSDEDLLAACRAVRADDLVQRLGGLG
                     AELGHGALSAGEAQLIALARVYASPASVVILDEAMSHVDPATEGGVERAFAERCAAGGG
                     VLVVIAHRLSSALRADQVLVMDGAATLLGRHDELLGSSPRYRAMMDAWTGPLPKPSSA"
     gene            complement(16442..17137)
                     /locus_tag="HUW46_RS23535"
                     /old_locus_tag="HUW46_04659"
     CDS             complement(16442..17137)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695558.1"
                     /locus_tag="HUW46_RS23535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04659"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_215549336.1"
                     /transl_table=11
                     /translation="MRFDDFDQRGYPEVDVRTGYGAWAATYDGSVLDAMDIALLEQLKE
                     PVWSTVDLAADLGCGTGRTGAWLRANGVSTVHGVDLTPEMLEGAGRRGAHAKLVEADVT
                     ETGLPSRSYQLITASLIDEHLDDLAPLYREAARLAAPGSAFVLVAYHPQFMMVSGMPTH
                     FTSADGESVTIATNLHLVSDHVGAALAAGWRLAEMREGLIDDHWVAAKPKWARFRGQPI
                     SVAYVWRND"
     gene            complement(17375..18337)
                     /locus_tag="HUW46_RS23540"
                     /old_locus_tag="HUW46_04660"
     CDS             complement(17375..18337)
                     /NRPS_PKS="Domain: PKS_ER (12-317). E-value: 1.9e-29.
                     Score: 94.7. Matches aSDomain:
                     nrpspksdomains_HUW46_RS23540_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630790.1"
                     /locus_tag="HUW46_RS23540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04660"
                     /product="zinc-binding dehydrogenase"
                     /protein_id="WP_215549337.1"
                     /sec_met_domain="ADH_N (E-value: 2.8e-05, bitscore: 23.1,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 4.5e-18, bitscore:
                     64.6, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAITATRFGAPDVLELVELPTPEPKAGEFRIDVEAAGVGWLDAL
                     IRSGHGPDVFEVEPPYVPGGAVAGTVTAVGAEVPDGWAGARVIARAGSGYGGGYADTAI
                     SAPDAAFRVPDGLDPRTALAVMDDGSTAVALLEWTPVRSGDRVLVAPGAGGLGNLLVQL
                     ALAAGAKVVAGVRGPEKAAIARDLGAEAVDYAEPGWPDRVRQLTGGLDVAFDGLGGAVG
                     AAAVDLLVDGGRFSGYGMTSGAEAVIGEADRQRLTVLDMGQLPEFWADNARRVQLVLAE
                     AAAGRLRPVIGRTYPLAEAAAAHEDIEARRFVGKSLLLP"
     aSDomain        complement(17387..18301)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HUW46_RS23540_PKS_ER.1"
                     /evalue="1.90E-29"
                     /label="HUW46_RS23540_PKS_ER.1"
                     /locus_tag="HUW46_RS23540"
                     /protein_end="317"
                     /protein_start="12"
                     /score="94.7"
                     /tool="antismash"
                     /translation="DVLELVELPTPEPKAGEFRIDVEAAGVGWLDALIRSGHGPDVFEV
                     EPPYVPGGAVAGTVTAVGAEVPDGWAGARVIARAGSGYGGGYADTAISAPDAAFRVPDG
                     LDPRTALAVMDDGSTAVALLEWTPVRSGDRVLVAPGAGGLGNLLVQLALAAGAKVVAGV
                     RGPEKAAIARDLGAEAVDYAEPGWPDRVRQLTGGLDVAFDGLGGAVGAAAVDLLVDGGR
                     FSGYGMTSGAEAVIGEADRQRLTVLDMGQLPEFWADNARRVQLVLAEAAAGRLRPVIGR
                     TYPLAEAAAAHEDIEARRFVGKSL"
     CDS_motif       complement(17654..17686)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HUW46_RS23540_0002"
                     /evalue="1.60E+00"
                     /label="PKSI-ER_m5"
                     /locus_tag="HUW46_RS23540"
                     /protein_end="228"
                     /protein_start="217"
                     /score="1.8"
                     /tool="antismash"
                     /translation="GGAVGAAAVDL"
     CDS_motif       complement(17846..17908)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HUW46_RS23540_0001"
                     /evalue="6.00E-04"
                     /label="PKSI-ER_m5"
                     /locus_tag="HUW46_RS23540"
                     /protein_end="164"
                     /protein_start="143"
                     /score="12.5"
                     /tool="antismash"
                     /translation="GDRVLVAPGAGGLGNLLVQLA"
     gene            complement(18371..19672)
                     /locus_tag="HUW46_RS23545"
                     /old_locus_tag="HUW46_04661"
     CDS             complement(18371..19672)
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947056.1"
                     /locus_tag="HUW46_RS23545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04661"
                     /product="family 1 glycosylhydrolase"
                     /protein_id="WP_254126487.1"
                     /transl_table=11
                     /translation="MARRWKSAAIAIAVLTAAAQAPVAAASVPIPRMPPGFHWGVSTAG
                     FQAEGSPPDSNWRRYVDGRADSVKDPYANAVDFRHRYAEDIALAKSMGVTTFRMSVEWP
                     RIEPRPGALDQDALAYYDDVVREIKAAGMTPMITLTHFTHPGWIADRGGWTNPGTVESW
                     LAFTDLVVRRYAGLGVLWVTFNEPTVYRTFEERNGKLGVFQSGAMMDNLVAAHRRGYDL
                     IHRLDPGAMVTSNLAVKPPPLQWFDDSSFVDKVRGKLDFVGIDYYYGASLDNLSVIHAA
                     KDEFWKVAPQPDGIYYALRTYQRKFPGLPLYVVENGMPSDDGKPRADGYTRSDHLRDHL
                     YWVQRALADGVRVIGYNYWSITDNYEWGSYRSRFGLYTVDALTDPALTRRPTDAVATYR
                     AAIAANGVPGGYLPKRRPAWCAFADLRTCFGRLP"
     gene            19727..20311
                     /locus_tag="HUW46_RS23550"
                     /old_locus_tag="HUW46_04662"
     CDS             19727..20311
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="HUW46_RS23550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04662"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_215549338.1"
                     /transl_table=11
                     /translation="MSGETRERILDEALKQFAERGFDGTTISSIERRVGLAGGTGSFHR
                     HFPSKRAVFDAAIERELTRLRTIRPEREPRADAPLERRLESDLEFLREVRSLIEILIRE
                     RATMPHLVERVGEVLVDGGLAGITSDLFPVGSHEDPAAAATIMLCATVGYFLTGEFFGT
                     PPGGVDPARYAAALAAMLSPRGRPRRAGSGR"
     gene            complement(20268..21002)
                     /locus_tag="HUW46_RS23555"
                     /old_locus_tag="HUW46_04663"
     CDS             complement(20268..21002)
                     /NRPS_PKS="Domain: PKS_KR (7-173). E-value: 3.8e-12. Score:
                     38.4. Matches aSDomain:
                     nrpspksdomains_HUW46_RS23555_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698214.1"
                     /locus_tag="HUW46_RS23555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04663"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_215549339.1"
                     /sec_met_domain="adh_short_C2 (E-value: 2.9e-34, bitscore:
                     118.2, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGTLAGKTALVTGGSRGIGRAVVLRLVSEGAKVLFTFAGNQTAAD
                     EVVAAGGGEAIAVQAHQGKLADIERLFAAVDERLGGLDILVNNAAANPMGGIADATEAD
                     FDEVIAINTKGPFFILQAAVKRLRDGGRIVNLSSSGSTPGLSLYFASKAALDAITAVAA
                     RELGSRGITVNGVSPGATETDMMRAAAGDHLEQAVEYVTAQTALGRLGQPEDIADVVAF
                     LAGPDSRWITGQNLRVSGGLAG"
     aSDomain        complement(20484..20981)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HUW46_RS23555_PKS_KR.1"
                     /evalue="3.80E-12"
                     /label="HUW46_RS23555_PKS_KR.1"
                     /locus_tag="HUW46_RS23555"
                     /protein_end="173"
                     /protein_start="7"
                     /score="38.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGGSRGIGRAVVLRLVSEGAKVLFTFAGNQTAADEVVAAGG
                     GEAIAVQAHQGKLADIERLFAAVDERLGGLDILVNNAAANPMGGIADATEADFDEVIAI
                     NTKGPFFILQAAVKRLRDGGRIVNLSSSGSTPGLSLYFASKAALDAITAVAARELGSRG
                     ITV"
     CDS_motif       complement(20904..20975)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HUW46_RS23555_0001"
                     /evalue="1.10E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="HUW46_RS23555"
                     /protein_end="33"
                     /protein_start="9"
                     /score="21.2"
                     /tool="antismash"
                     /translation="LVTGGSRGIGRAVVLRLVSEGAKV"
     tRNA            complement(21271..21344)
                     /anticodon=(pos:complement(4902222..4902224),aa:Phe,
                     seq:gaa)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="HUW46_RS23560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="HUW46_04664"
                     /product="tRNA-Phe"
     gene            complement(21271..21344)
                     /locus_tag="HUW46_RS23560"
                     /old_locus_tag="HUW46_04664"
     tRNA            complement(21520..21593)
                     /anticodon=(pos:complement(4902471..4902473),aa:Asp,
                     seq:gtc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="HUW46_RS23565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="HUW46_04665"
                     /product="tRNA-Asp"
     gene            complement(21520..21593)
                     /locus_tag="HUW46_RS23565"
                     /old_locus_tag="HUW46_04665"
     misc_feature    21600..21616
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.92"
                     /tool="antismash"
     tRNA            complement(21616..21688)
                     /anticodon=(pos:complement(4902566..4902568),aa:Glu,
                     seq:ttc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="HUW46_RS23570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="HUW46_04666"
                     /product="tRNA-Glu"
     gene            complement(21616..21688)
                     /locus_tag="HUW46_RS23570"
                     /old_locus_tag="HUW46_04666"
     misc_feature    complement(21811..21824)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 20.8"
                     /tool="antismash"
     misc_feature    21915..21930
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.78"
                     /tool="antismash"
     gene            complement(22257..22496)
                     /locus_tag="HUW46_RS23575"
                     /old_locus_tag="HUW46_04667"
     CDS             complement(22257..22496)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642957.1"
                     /locus_tag="HUW46_RS23575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_04667"
                     /product="hypothetical protein"
                     /protein_id="WP_215549340.1"
                     /transl_table=11
                     /translation="MSGSVEVRQFLLRHEGGDMSGGVPAQRSGSSDSQRVSDDQVRRAR
                     LAVARGANGVEDCSQLLDMLGLNPDDDGQAPVQR"
ORIGIN
        1 aagatcccga tccccggaat ggcggtgtac aacgcgagca agttcgccgc ggtcgggctc
       61 accgccgcgg tccgccgcga gctggacggc accggcgtga gcgtcagcgc ggtgctgccg
      121 tcggcggtgc gcaccgaact gtcctcgggg gtgccgctcg gcaagggcat ggcgacggtg
      181 gaccccgagg tgatcgcggc cgcggtggtc cgcagcgtgc gcagccgcag ggccgagatc
      241 ccggtgcccg gctacctcgg cgtgtgggac ctcctcgccg cggtggtgcc cgagccggtg
      301 atgcggttcg gcaggcggct cgcccgcgac gaccgcgcgc tcacgcagat ggaccacgag
      361 gtgcgcgccg cctacgaacg ccgcgtcgcg ggacaatccg gcgaataacc gcgtggactt
      421 cgttcacacg gcgttgttaa gctccggtca tgtccacccc tgaggcatcc agactctagc
      481 caccgatttt ccggtggctt tcgcggcgcg cgtttcccgt gtcgctgaac cctgccatcg
      541 gaacggctcg tgtcctcacc ccgccctttc gcggggttcc tccggtggtg ggcgtccctc
      601 ctttgtggac agccgccgcc cttcggtgtg cggagttctg tcatgccttt cgctgtgtac
      661 atgctcggcc tcgcggtttt cacgcagggg acctccgagt tcatgctttc cggtctcgtc
      721 gcggacatcg cgcgggacct gcacgtgtcc atccccgccg cgggcctgct gacctcggcg
      781 ttcgccgtcg gcatggtgat cggcgcgccg ctgatggcgg tgctgggcct gcgctggtcg
      841 cggcggcgcg cgctgaccgg tttcctggtc gtgttcatcg cggtgcacgt cgtcggcgcg
      901 ctcacgcccg gctatgaggt gctgttgtgc gccagggtgg tgggcgcgct cgcgaacgcc
      961 ggattctggg ccaccgcgct cgcgacggcg aacgcactgg tggagccggg tttgaaagcc
     1021 cgcgctacgt ccactgtggt cggtggggtc accctctcct gcgtcgtcgg tgtgcccgcg
     1081 ggcgcgctgc tcggccagta ctggggctgg cgcgccgcgt tctgggccgt cgcggttctg
     1141 tcggtacccg cggtgttcgc gatcctgcgc gccattcccg gcggcaggcc gccgtcggcg
     1201 tcgggaacga ccgcgcgcga ggaactgcgc gtgctgcgga acccgcgggt gctggtggtc
     1261 ctgctgctgg gcgcgctcgt caacggcgcc acgttctgta cgttcaccta tctcacgccc
     1321 ctgctcaccg gggtcaccgg attcggggag ggctggatgc ccgcgctgct cgcgttgttc
     1381 ggcgcggggt cgttcgtcgg ggtcgcggtg ggtggccgga tcgcggacgc gcggccgctc
     1441 gcgctgctgt gggccggttc ggccgcgctg tgcgcggggt gggtggtgtt cgcgctggcc
     1501 gccggggttc cggtgctggc ggtcgtgttc gtgctggtgc agggaatcct gtcgttcggt
     1561 gtcggctcga cgctggtcgc gcgggtgttc tcggtggcga ccggggcgcc gacgctcgcg
     1621 ggcgccctcg cgacggccgc gttcaacgtc ggcgcgaccg cgggcccgtg gctgggaggg
     1681 ctcgcgatcg gcgccggcgg cgggttccgc tcgccggtgt gggtgagcgc cgcactggtc
     1741 gcgctcgcgt tcgccgtctt cgcgctgagc agggtgccga cccggagccg gacgcgctga
     1801 aaagcccccc ggcgtacgaa ctctaccttt cgttcgctgg ggggaccccc gatttcgagt
     1861 ctattcgagg gcaccgacat ttttcggggc cgcggagccg tcaccgggtc tatcgtcggt
     1921 ccatgtggcg cacgacgggg ctggcgcgcg gtgacgggtt cgcgctcgac gaactgcgca
     1981 tcgtcgagcg cgcgccctcg tggtcggagc cggtgccgag cgccgggcac cggctggtct
     2041 tcgtgcggcg cggccttttc cggctccggc tgccccgctg gacggggctg gtggatcccg
     2101 gcaccgggta cgcgagcggt cccggtgacg agcaggcgat cgcgcaccgg cccggccgcg
     2161 aggacgtgtg caccgtcgtc accgtcgccg agtcgatgct gccggacggc tgggccccgt
     2221 gcgcgccggt gccggtcgcg gcgcgggtcg atttcgcgca ccgcgtgctc gtcgcacgga
     2281 cgcgagcggg cgcggacgag ttcgaactga ccgagcgcgt ggtgcgcctc gcgggcgccg
     2341 tgacgtggcg cgccgccccg aaatcctcgc ccgcgcaccg ccgtctcgcg gaggccgcgc
     2401 gcgaagcact gctcgaagat cccgctcgcg ccacgatcgg cgagttggcc gcgacgctgg
     2461 ccgtctcgcg ctcgcacctc agccgggtgt tccacgagga gaccggtcag acgctcacga
     2521 ggttccgcgc cggggtccgg gttcgcgccg cgctggaccg gctgaccgag ggagaggccg
     2581 atcttgccgg gctcgccgcc gatctcggct tcgccgatca cgcgcatctc accaggaccc
     2641 tgcgtgacca cctcggcctg ccgccgagca aggcccgcga actgctgagc acgaatgttc
     2701 aagccgtggc tgccgaaacc ggccacgctc gcggtgacaa ccgaggaggg cgtgatgatc
     2761 ggacgactgt ggcaggcacg ggcggaaaac agcgcgaaag ctgacgccta cgaagaggtg
     2821 ttccgcggtg cggtgctggc cgaattgcgg gacctcaggg gtttccgcgg cgcctacctg
     2881 ctgcgccggg acaccggatt tctgacgatg accctgttcg acggtctcga cgacgtgcgc
     2941 gggttcacgg ggcgggacgt cgaaacggcc aacgtgagcg gagcggcccg cgccgtgctc
     3001 gccgatttcg acaccaccgc acggcatttt cgcgtggtcg tcgatccgtc cttttcggac
     3061 ggtcattgag gacggtcgtc agcgcagccg cctgcgcagg acgatgaaca tcctggcccc
     3121 gaacgcggtg ccgcacaacc ccatcgcgac ctggaccagg ttcggtctga gcaggccggt
     3181 gtaggcgacg aacagcccgg caagcggcag tgccgcgtag aacgccagcg tccacccgct
     3241 gaccggtggc ttctccatgg cgggcgacct tagcccgtcc cggccgcgat gcccatcgcc
     3301 tgcccggttc cggtcatccg gttgacatgc aggtatttac ctgcataatc ggagaggtgc
     3361 tgaacgaggt cttcaaggcg ctcgccgacg aaacccggcg gtacctgctc gaccggttgc
     3421 acgagcggaa cgggcagacg ctgggcgagc tgtgcgaagg cgtcgggatg acgaggcagt
     3481 ccgcgaccca gcacctgcag gtgctggagg cggccaacct gatcagcgtc gtgcgccgcg
     3541 gccgcgaaaa actgcactac ctcaaccccg ttccgctgca cgagatccag gaacggtgga
     3601 tcgacaagta cgagcggccg cgtcttcgcg cgctgagcac gctgaagaac cgagcggaga
     3661 acctcatgac gaaaccgtcc ttcgtctacg tgcccacccg tcacccagcc accacccccc
     3721 aaggaatcgc ttcgcgatac gtgatctata tcgaaagcac cccggaaaag gtgtggcagg
     3781 cgctcaccga cgccgatctc accgctgaat attggggcca ccgcaacgtt tcggactggc
     3841 agcccggttc ggcgtgggag caccagcgca ccgacgggtc cggcatcgcc gacgtggcgg
     3901 gcacgatcgt ggagagcacc gagcccgcga aactcgtgat cacctggtcc ttgccgggca
     3961 cggatggtga gccgtcccag gtcaccttcg acatcgagcc gcacggcgag atcgtccggc
     4021 tcaccgtgac ccacgaaaac ctcgccgacg aagccgagcg cgaaagcgcg gccaagggct
     4081 gggccgccgt gctgtcgaac ctcaagtcgc tgctcgagac cggtcacgtc ctgccgaggg
     4141 cgccctggga gatgtgactc gcttcacggc ccgcgctgtc acacattccg ctcctggtca
     4201 gtcttcatgg tgcgacccac aggagggagt cccgaaatga cagcggaaaa gatcgtcgtg
     4261 gtcggcgccg gatacaccgg actggccgcg gccaagctgg ccgcgaagtg gaccggcgcc
     4321 gaggtgaccc tggtcaacgc gagcgaccgc ttcgtcgagc gggtccggct gcaccagctc
     4381 gccagcgggc agcggttgcg cgacctgccg ttgcgggacc tgctggcggg caccggcgcg
     4441 cggctcgtcg tgggccgggt tcgcgcgatc gaccccgacg cccgcgaagt gctgctcgac
     4501 gacggcgccc cgatcggcta cgacaagctc atctacgcgc tcggcagcca cgcgaacctc
     4561 gacggcgttc ccggtgcggc ggaacacgcc tacaccgtcg cgagccgagc cgacgcggaa
     4621 cgcttgcgcg ccaagctttc cgggagcagg accgtcgcgg tggtcggcgg cgggctgacc
     4681 ggtgtcgaag cggcggcgga actcgcggaa gcccacccgg ggctgaaggt cagcctggtc
     4741 accggcggcc cgctcggcgc ggcgctgtcc gaacgcggca cccggcacct gcaccgcgtg
     4801 ttcggcaggc tcggcgtcga cgtcagggaa aacgcgaagg tgatcgaagt ccgcgccgac
     4861 gggctcgtgc tcgccgacgg tgacacggtc gaggccgaca cggtcgtctg gacggccggg
     4921 ttcgtggtgt cgtcgatcgc ggcggaggcc gggttcgacg tggacgcggc gggcaggatg
     4981 gtcgtggacg aggcgctccg ctcggtctca caccctgatg tcatcgcgat cggggacgcc
     5041 gccgcgatcc gcctcgccaa cggcctcgaa ctgcggatgg cctgcgccac cgggcttccg
     5101 accacacagc acgccgtgcg agtgctcgcc gggaaggcca cgggctcccc gcggttccgg
     5161 tacttcaacc agtgcatcag cctcggccgc cgcgacgcgc tgatccagtt cgtgcgcgcc
     5221 gacgacagcc cggtcgatgc cgtgctgaag ggcaggatcg ccgccttcta caaggaagcg
     5281 gtggtacgcg gcaccatcct gttcgaacgc caccccgtgc tgccgacttc accgtgacac
     5341 acccggacac cgaacgtcgg atggtgccgc aggctcccct gccccgaaag tgaccttcgg
     5401 ggacctgagc gccccgaagg ccactttcgc ggcatgggcc ctgtgattgt cgggtgcgcg
     5461 aggggcggat ttgtggcgtc taacgccccg aaggccacct tcggggcacg agccacctcg
     5521 cccgcatgcc tgcgagggcc gagaaagtgg cgctagattc cccgaaggtg gccttcgggg
     5581 accacggcct cctccccatc actcctctcg cccaatcacc gcgacccgca cccgacagca
     5641 aagcgacctt cggggacctg agcgccccga aggtcacttt cggggcgtcg agcgggatcc
     5701 ccgccgcgga atgaacaaca gggcgcccac tccgcccacg agcatcacca gcgccatgga
     5761 cagatcggag gcgccacccg cgctgccgag gccgacgagc ggtccggcga tcccgcccgc
     5821 gacgaactgc agcacgccga gcaatgccga agcgctgccc gcgatcgacg ggtggtccgc
     5881 catgcccagc gcggtgcagt tgggcgcgac cagacccatc gcggccacgg cgacgaacat
     5941 cggcggcagc agcgcgggca ggccgagccc ggcgagcgcg gcggcggtca gcgccaccac
     6001 ggcgacggcc ccggccacca cgccgatgat cagcatgcgg cgcggcgggt agcggcggac
     6061 cagccacgcg ttggactgcc ccgcggcgag caacccgatg gcgttggcgc cgaacacgac
     6121 gctgaattcc tgtgtggaca gtccgtggcc ctgttcgagc acgaacgccg agccggacag
     6181 gtagccgaag taccccgcga agacgaggcc cgaagtgagc gcgtagccga ggaaggtgcg
     6241 gtcggcgagc aaggtccggt aggcgcgaag gctcgtcgac aacccgccgg tgtgccgcgc
     6301 ttccgacggg agcgtttcgg gcagccacag cgcgcaggcc acgagcagca ccgccccgaa
     6361 caccgcgagc gcgacgaaaa ccccgcgcca cgaaccgaaa gcgagcaact gcgcgccgag
     6421 ggccggggcg agtatcggcg cgaggctcgt cactagcatc agcatgctca cgagccgcgc
     6481 catcgccgcg ccggaggtca gatcgcgcac gatggccctg gccagcaccg gcccggcggc
     6541 accggcgaac ccctgcgcga accggagcgc gatcagcacc gtcgcgttcg gcgcgatcaa
     6601 gcagacgagg gaaagcacgg tgtagacggc gagtccggcg agcagcggcc gccgcctgcc
     6661 gaaggcgtcg gacagcgaac cgaggaccag ctgcccgaac gcgaggccga ggatgctcgt
     6721 ggtcagcgtg agctggacct gggacgcgcc ggtgtgcagg tcggcggcga tctgcggcat
     6781 cgacggcagg tacatgtcga cggacatcgg cgcgcacgcc gacagcccgc ccaggatggc
     6841 gacccggccc gcggtcggcc gataggtgga gaccccagtg acgctcatgg ccgcaagctt
     6901 agggggaacg gagcattccg ttccaccctc gtatgctgtg gcccatgtct cccgaaccgc
     6961 agacgcgagc gcgccggagc gacgcctacc gcaacgacga gcgcatcctc gtcgcggcgc
     7021 gggaagtgtt cgccgagctg ggggcggacg cgccggtgtc cgcgatcgcc gaccgggccg
     7081 gggtcggcat gagcgcgctc taccggcgct acccgagcaa ggacgacctg atgcgcgcga
     7141 tcatgcgcga caccatggag cggacggaac gggaagcgct gcgcgcgctc gacgagccgg
     7201 acgcctgggc gggcttcctg ggtttcgtgc acgcgtgcgc agacgcggga atcaccggcg
     7261 cgccccggat ttcgggtgcc gtccggccca ccgaggagat gctcgcgcgc agcaagcggg
     7321 cgcgcgaagc ggtgcaggcg ctcgtcgaac ggacgcaggc ggaaggcggc ctgcgcgcgg
     7381 acgtcaacgc gcacgacatc gtgttcctgc tcggcgagct gcggagccag cgggcggcca
     7441 aggtgatcgc gccgcccggc gtgcaggagc gcctgctcgg cgtggtcctc gacggcctcc
     7501 gcgcccccaa cgcgactccg ctgaccgccc ggccgggtac ctgggaggac atgcacgagg
     7561 cgtggcgggc ggcccagcga gaacacggat gacctactac gtccggtagc attggcgagc
     7621 tggggcaggg cggacgaggg agctttcgtg ggcaggcttg gcgagctcga acgcgcggtg
     7681 atggacgcgc tgtgggatcg cggcgaaccc gccaccgtcc gcgtcgtgca cgccgacctc
     7741 gccgagcgcg atctcgccta caccaccgtg atgacggtgc tgaccaggct cgcggacaag
     7801 gggctcgtcc ggcgcgagcg ggacggcagg gcgtggcggt acgtgcccgc cgccaagcgc
     7861 gaggactaca tcgccgagct gatgctggaa gcgctcgccc tcaccggcga ccgtggcccc
     7921 gcgctcgtcc ggttcgcgaa ctcggtgagc accgacgaag ccgaagcact gcgtcgggcg
     7981 ctcgaaaacc cgcgatgatc tacgcattcc accacttcgt cacgttcggc atggccgtgt
     8041 cggcgatgtg gtggctgccg cggggcacct ggacgcacgc gagcccgcgc accgcgctcg
     8101 tgctgtggca gctcgtgctg tgcgccggcg tggtttccgc gatcgggctg ctgttcgaac
     8161 tcgggctcga gccgtaccac ctgggggtac tgcccggcct cgcgcgcgtc gtcgacgagg
     8221 ggcacgtgcc gaacccggtg atcctggccg cgaccgcgct gaccgtatgg ctcgtggtga
     8281 gcctgatccg gctggtggcg agcaccgcgc gcgtgcgggc ccggcaccgc gggctgctcg
     8341 cgctcgtcgc gcgcaccgac ggcgacgcgc tcgtgctgga ccactcggcc gcggtcgcct
     8401 actgcctgcc gggcaggcaa ccccggatcg tgatcagcgc gggcgcgcgc gaactgctca
     8461 ccgacgacga gctgcgcgcg gtgctcgcgc acgaacgcgc gcacctgcgg cagcggcacc
     8521 acctcgtgct cgaaccgttc caggcgtggc ggctgttcgt cccgcgccgg gcgctgtccg
     8581 cgatcgggct gctggtggag atgtgcgccg acgagtcggc ggcgcggcgg cacggccgcg
     8641 cggtgctcgc ttcggcactg gagaagttcg gcacctcggc ggccccggcg ggcgggctgg
     8701 ccgcgggctc ggtcggcacg ctcgcgcgga tcgaacggct gcggcacccg cgcccggccg
     8761 ccgcgctcgg cgtccgcctg ctggcggtgg cgaccgcctt ggccgcgctg ctcaccccgc
     8821 tgaccctctt cgtctggccc tcctgaactg cgtttagcgg gctaaacgca ctccggacga
     8881 ccgggaatca gcgggtgcgg cgcagccagt agaggccgat caccggcagc accagcggga
     8941 tgaagaggta gccctgcccg tagcccgacc acacggtcgc gtgcgggaac gcggcggagt
     9001 cgaagacgct gagcgtgccg acggtgagca cgccgacgaa ctccacaccg cacgcgatca
     9061 gcgcgaccag ccacgacgtc cgcccggagc gggcgagcgc gacggtcgcg accaggtaca
     9121 ccacggccgc gaacgcggac agcaggtagg cgagcggcgc ttcgtggtac ttggtggaca
     9181 gctgcaccgc cgcccgcgaa gtcgcggcga gcgcgaagat cgcgtacacc gcgacgagga
     9241 tccggcccgg cccggtcgcg gtcttcttct cgtcacgcac tcgcgccatg ccacacctcg
     9301 ctcaaccgca ggaccatgac cgggatggcg acgcaggcga tcccgagcac caccgtgctc
     9361 gaccggctcc gttcggcgag cgcccacacc gtgccgacgg gcagcacgac gagcgagccg
     9421 atcaggtagg ccaggtaggt cgccatgctg ccgggccggt ccccgccgac gagcagcacg
     9481 acaccgatca ccagctgcgc caccagcagc agctcgacga atccgagcgc gtacagcagc
     9541 ggccgccggg gttcgcgccc acgcgcggcg agcacgaagc tccacaccgc caccgcggcg
     9601 gcgcacgcgg cgacggtcac gccgaatcct gtgatcaccg gtcctccaat actacctttc
     9661 gtagtatagg aaaccgcctc cgaacgggtc gcaccggctt gccgcggagc gctttggaaa
     9721 gaaacaaacc caccgcgggt gacgggctgc gacgtttggt gccgcgggtg tcacctgccc
     9781 ttattccggt gccggattct gttacacgtc atcagggtgg ttggtctgga cctcgctttc
     9841 acccagagtt gggttactgg ctggttggcc gcctaaatgc ctacctcgaa cagcgcagcc
     9901 gaaaccccga tatggcggat tggccgtcgc gtgccggttg tgcgggaatg gttccggcac
     9961 cggaaagagt gcctgcgatc gagtgacgga agggaacccg atggacctcc gctacgaggc
    10021 gttttgcttc gcggatccgc tgttcttcga cgaacagcgc gaatcgggtg caccggacga
    10081 tgatttctcg cgagcccttc cggtgccaga cgggtgggtc gcttccgatc ggggcatctg
    10141 gcggtcgttg catcggctgg gccgccagct tccgttgcag gggtggaaaa ttcacgtgag
    10201 cgccaacctc ggcaacgccg acgaggtgct ggcctccgtg taccgctact gcgttcgcga
    10261 cgattattcc ttcaagcacc tcagatcaag atcgatcttg ctcgcccgca attcgaaata
    10321 cgcgccgcgg gaggcgagtg gcaagctcgt cactatttat ccggcggaca acaaagagct
    10381 ggaacgtgtt cttgtcgatc tttcatccgt gctggccggg cagtccggcc cctatattct
    10441 cagtgatctt cgctacggcg acgggccgct ctacgtccgg tacgggggat tcgccgagca
    10501 gtgggtcgag gcggacggcg ccagggtcct ggcgatcacc aaaccggacg gcacactggt
    10561 gcccgacgag cgcgggcccc gtttcgtggt gccagactgg gagccgctgc ccgaatgcct
    10621 cgtcccgcac ctcgcggcgc gcaagggggg cgatccgcgg cagttcccgt accgcgtgac
    10681 ctcgtcgctg cacttctcca acggcggcgg cgtctacctg gctgaccggc tcgccgacgg
    10741 gaagcggctc gtgctcaagg aagcgagacc gcacgcgggt ctcgaccggg ccggggtcga
    10801 cgcggtggcg aggctgcgcc gcgagcacga cgtgctcgac cggctccgcg ggatcaccgg
    10861 tatccccgag gtctacgacc tgtttcccgt ctgggagcac cacttcatgg cgatggagca
    10921 ggtcagcggg ttgtcgctgg gctcgtggct ggccaggaac tacccgctga ccaggcgcga
    10981 cgcggaacac accgacgttt cgggttaccg ggcccgtgcg atggcgctcg tcgcggaggt
    11041 cgagcggatg gtcgccgaag tgcacgagcg cgggatcgtc ttcggcgacc tgcacccgct
    11101 gaacgtcctc gtcgaaacga cggaagcgac cgaaaccacc ggcgaggtcg acgcggtgtc
    11161 gatcatcgac ttcgagatgg ccttcgacgc ggacagcggt gaccggcccg cgctcggcgc
    11221 gccgggcttc cgcgcgcccg ccgaccgctc gggtttcgag atcgacgcgt acgcggtagc
    11281 cgcgctgaag ctctggatct tcttgccgct gaacacgatc ctcgacttcg aaccgggcaa
    11341 gctgcacagc ctggtcgact tcgccgccga gcggttcgac ctgccgaagg gctacgtcga
    11401 cgagatccgg cagggcctgg ccccgcgatc cggcggcagt accgcgaccg cgaccgcgct
    11461 cgacgacccg gtgccggact ggcagctcgt ccgcaagaac atcgggcagg cgatactggc
    11521 gagcgccacc ccggaacgcc aggaccgcct gttccccggc gacatcgagc agttccgcgt
    11581 cggcggggcg tgcttcgcct acggcgcggc gggcgtgctg cacgcgctcg acgccgcggg
    11641 cgaaggccgg taccccgagc acgagcgttg gctgctcgac cacgtgcgac gggaaccgcc
    11701 cgcgcgcccc ggtttctacg acggtgcgca cggcatcgct tacgtgctcg agaacttcgg
    11761 ctacgcgcgc gaagcggacg cactgctcga cggcgcggcg accatggtcg agcagaccaa
    11821 ggagcacagc ctcgacaccg gtctcgccgg aatcggcctg aacttcctgc acttcgcggg
    11881 cacccgcggc gacaccgagc acaccaaacg cgcgctcgcc atcgggaaac ggctggccga
    11941 agcgctgccg tcggccgaac cacccggaaa gttcggcagg gcgggcctgc tcagcggctg
    12001 gtcgggcccg gcgctgctgt tcgtgcggct ctacgaatgc accggcgaag agtcgtggct
    12061 cgcgctcgcc gaccacgcgc tccgccgcga cctcgacgaa tgcgtggccg ccgacgacga
    12121 gtcgctccag gtccgcgacg gcgaagcacg cacgctgccc tacgtcggca tcggcagcgc
    12181 gggcgtcgcg ctggtcgccg aggagctcgg cgagtaccgg ccggacgcgg acagcgtcgc
    12241 cgcgctgccg aggctgttgc aggccagccg cggcgagttc gtcatccacc ccgggctcat
    12301 gttcggcagg gcgggcctga tgaccgcgct cgccgcgcgg cgcgaccgcg acgaggcgac
    12361 cgaacgcgct gtccggctgc acctgtcccg gttcggctgg tacgcggtcg cctactccga
    12421 cggcatcgcg ttcccgggga accagctgct ccggctctcg atggacctca acaccggcgg
    12481 cgccggcgtg ctcctcgcgc tcgccgccac cctcgacggt gcgcctgccc tgccgttcct
    12541 cggcggctcg caccgtccca cgaaccccgg tcgttcgtga ccggtggaaa tacacactag
    12601 cgaaaggcaa taaccatgga catggttctc gacctgcagg cgatggaagc ccccgagagc
    12661 gtcgacggcg gccacggcgg cggccacggc tccaacctct cgctgctggc ctcctgcgcc
    12721 aacagcacca tcagcctgct cacctgccac tagatctttt tggtgacggc ttgcgtagcg
    12781 gtgcgggtag cggaacctca gcagccttct cactccggga tctccaactc atgaatgcca
    12841 attcaccacg ttggagctgt cctcgcgaga agactcctga gaacccgcgg cggtgccagt
    12901 tgctggcccg ctcaagcggc tccgccgctt acgaaaagcc gggatagcag gtgtaacgac
    12961 tgcgcgggag cggcacaggc caagtgcgcc gctcccgcgc tttccattgt gtcccacccc
    13021 ggccgggcag gacaccgagt ccaaagggat gagatgcgcg caccgggcga tcggctgctg
    13081 agcgaggtga cctcgctcga acgcgcgcgg atgccgttca tcgtgctgag cgcactgctc
    13141 gccgcgggct ccgggctgct gctgccctcc gcgctcgcca ccgccgtcga cgcctcgatc
    13201 gcgggctccc ggagctggcc cgcgatcctg tggctgctcg cactcggcgc cgtccagatc
    13261 ggcgccgacg tcgcgggcgg catcctcacc gtgcgcgtca ccagctccgc gaccgcgtgg
    13321 ctgcggcgca cggtcgcgcg gcacttcctc gcgctcggca cgaaatcggg tttcgtccac
    13381 ggcgacgcga tcagcaggct caccggagac gcggtcgccg cgggaaccgt ggtgtcgatc
    13441 ctcgtgcagc tcgcgtccgc gtcggtcatc tcgtgcggcg cgatcacctt gctggcggtg
    13501 ctcgactggc gcctcgcgct cgtgttcgcg ggcagcgtgc cgctcgcgct cctgctcgca
    13561 cgttcccacc tgcggctcac cgccgatgat gtgcttacct accaacaggt ttcgggcgag
    13621 atctcggctc gcctgctcga cgcgaccgcg gggctgcgga cgatcgcggc gtcgggcacc
    13681 gccgaccgcg aagccgcgcg cgtgctcgaa ccgctgccgc gcctcggcgt cgcgggcggg
    13741 ggcatgtggc gcacgcaggc gcgcatggtg tggcgcgccg cgctgctgct gcccgccgtg
    13801 gaggtgaccg tgctcgtcgc cgccgggttc ggggtgctcg gcggcaggct gaccgtcggc
    13861 gatctgctcg cggcgctcgg ctacgtcgcg ctcggcatga ccatggtcaa ccagatcccc
    13921 ctgctcacca cgctcagcag ggcccgctcc ggcgcgacgc ggctggccga ggtgctcgat
    13981 tccccggtgc cgcaccaggg cacgcgcccg ctgccgcccg gccccggcac gatcgaactg
    14041 cgcgacgtcg gcctccgcgg cgcgctcgag cacgtcggcc tgaccgtgct cgcgggcacc
    14101 acgctcgccg tcgtcggccg gtccggtgcg ggcaagtccg cgctcgccgg ggtgctcggc
    14161 ggcctcgtcg aaccggacga aggcgaagtc ctgctcgacg gcgtgcccat cgcgagcgtg
    14221 cgcccggagg aactgcgcgc cgcgttcggg tacgccttcg aacgaccgtc gctgctgggg
    14281 gagaccgtgt ccgacgccgt cgggtacggc gcgtggtccg gaccgtccgc ggtgcgcgcc
    14341 gcctgccgga ccgcgcaggt gcacgatctc gtcgtccgcc tgccggacgg ctaccgcacg
    14401 ccgttgaccg aaaccccgct ctccggcggg gaagcgcagc gcgtcggcct ggcgagggcg
    14461 ctcgcccgcg gcccgcgcgt gctcgtgctc gacgacgcga cagccagcct cgacaccgtg
    14521 accgagtccc ttgtggacaa gtccatttcg gacaccatgc cgggcacgac caggatcgtg
    14581 gtgacgcacc gcgtcgcggt cgcgcggcgc gccgggatgg tcgcctggct ggaccatggc
    14641 aggctgcgcg ctgtcgggcc ccacgacacc ctttgggctg aatctggcta ccgcgcgatc
    14701 ttcaccgatg aggagccatg agcatccagc ggttgtacgg ggccgcgctg gtgggcaggt
    14761 ggcgcgccgc tgccgccctg ctcgcgtgct cgattctcga aggcgcgccg gcgttcttct
    14821 ccggcaggtt cgtggaactg tccgtggaca agggtttcgg cgtgggcagc gcggggatcg
    14881 gtgtcgcgtg gctgctggtg ttcgccgggg tcgcggtgct cggcgcgctc ggttcgcggc
    14941 tggtgtggca ccggctcggg acgatcgtcg agccgatccg ggacgcactg gtccgcgcgg
    15001 tggtgcgggg tgtgctgcac gacgcgaacc cgccgcgcaa ccagcccgac gcgagcgggg
    15061 tcgccaggat cacccagcac gtcgaagtgg tccgcgacgc gaccgcgggc ctgctggtgc
    15121 aggcgcgcgg catgctcgtg accacggccg ccgcgctcgc cgggctgttc accgtcgccg
    15181 gttcgctggc ctggctcgtg gtcgtccccg tcgtactggc catcacgatg ttcgggttcc
    15241 tgctccccgc gctcgccacg cggcagcgag cggtcgcgct cgccgacgaa gcgctggccg
    15301 aaaccgcggg ctccgcgctc gccgggatgc gggacgtggt ggcgtgcggt ggcgaggcga
    15361 tcgcgggaat ggcgctctac gaacgggtcg gcgcgcaggc gtcggccgcg atgcggctgg
    15421 cgagggcgtc ggcgttgcgg acgctgacga tcgcggtcgg cgggttcgtg ccgctgctgc
    15481 tggtgctggc cgcggcccca ggcatggtcg cggcgggctc gatgaccgcg ggcgccgcgc
    15541 tcggcgcgct cgtctacctg gcgaacaccc tgcagcccgc gttgcacggg ctcgccgcga
    15601 ccgcgagcac ggtcgtgctg cgcctgctcg tcgcgttgcg caggctcgac gagacgagcg
    15661 acgtgccgac gccgcggcag ggcggcgcgg aaccggcgaa cagcacgatc accgtgcggg
    15721 acctcacttt cggctggggc gcgcacgccg agccggtggt gcgcggcctc gatctcgatc
    15781 tctcgccggg cgagtacctc gcggtggtcg gcccgagcgg gatcgggaag tcgacactgg
    15841 ccgggctgct caccggattg ctgtccccgc agggcggccg cgtgctgatc ggcggggtgc
    15901 cggtgggtgt cgtgcgcccg gccgcgctgc accggatggt cgcgctgagc ccgcaggagt
    15961 cctacgtgtt cgccggtacg gtgcgggaaa acctcgcgct gttcggtacc ggactgtccg
    16021 atgaggactt gcttgccgcg tgccgcgcgg tgcgcgcgga cgatctggtc cagcgcctcg
    16081 gcggactggg cgcggaactc ggccacggcg cgctctcggc gggggaagcg cagctgatcg
    16141 cgctggccag ggtctacgcg agcccggcgt cggtggtcat cctcgacgag gcgatgtcgc
    16201 atgtggaccc cgcgaccgag ggcggggtgg agcgggcgtt cgccgagcgg tgcgcggcgg
    16261 gcggtggggt gctcgtggtg atcgcgcacc ggctgtcctc cgcgctgcgc gccgaccagg
    16321 tgctggtcat ggacggcgcc gcgaccctgc tcggcaggca cgacgaactg ctcggatcct
    16381 caccccgcta ccgcgcgatg atggacgcgt ggaccggccc gctgccgaag ccctcgtccg
    16441 cctagtcgtt gcgccagacg taggcgacgg aaatcggctg gccgcggaac cgcgcccatt
    16501 ttggcttcgc cgccacccag tggtcgtcga tgagcccctc gcgcatctcg gcgagccgcc
    16561 agcccgcggc gagcgcggcg ccgacgtggt cgctgaccag gtgcaggttg gtggcgatgg
    16621 tcaccgattc gccgtcggcg ctggtgaagt gggtcggcat cccggacacc atcatgaact
    16681 gcgggtggta ggcgaccagg acgaacgcgc tgcccggcgc cgcgagccgc gccgcctcgc
    16741 ggtaaagcgg cgcgagatcg tcgaggtgct cgtcgatcaa ggatgccgtg atgagctggt
    16801 agctccgcga cggcaacccg gtctccgtca cgtccgcctc gaccagcttc gcgtgcgcgc
    16861 cgcgcctgcc cgccccttcg agcatctccg gcgtcaggtc cacaccgtgc actgtggaca
    16921 ctccgttggc gcggagccaa gcccccgtac gccccgtccc gcagccgagg tccgccgcaa
    16981 ggtccactgt ggaccacaca ggctctttca gctgctcaag gagcgcgatg tccatggcgt
    17041 cgagcacgga cccgtcgtag gtcgccgccc acgcgccgta cccggtgcgg acgtccacct
    17101 cgggataccc ccgctgatcg aagtcgtcga agcgcatacc cccgattatg accaccacct
    17161 ccgcccctgc cgaccgagtt ttccgccgct cgtacctcat tcccgcgcct ctgccgcaaa
    17221 gttgtccaca ctcggcgccc cctgtggaca actcgccttc acacccccgc cgctccccac
    17281 tccccggtag actgggctgg ggcagccccc cctgggagcg gcgggggaat gaactaggta
    17341 atgcggttca cgggcatgcg gacgtcgcga gggttcaggg cagcagcagg cttttgccga
    17401 cgaagcgccg cgcttcgatg tcttcgtgcg ccgcggccgc ttccgccagc gggtaggtcc
    17461 ggccgatgac cgggcgcagc ctgcccgccg cggcttcggc gagcaccagt tgcacgcggc
    17521 gcgcgttgtc cgcccagaac tccggcagct gccccatgtc cagcacggtc agccgctgcc
    17581 ggtccgcctc gccgatcacc gcttcggccc cgctcgtcat cccgtaaccg gagaaccgcc
    17641 cgccgtcgac gagaaggtcc accgccgccg cgccgaccgc accgccgagc ccgtcgaacg
    17701 ccacgtccaa gcccccggtg agctgccgca cccggtcagg ccagcccggc tccgcgtagt
    17761 cgaccgcctc ggcgccgaga tcccgcgcga tcgcggcctt ctccggcccc cgcactccgg
    17821 cgacgacctt cgctcccgcc gcgagcgcca gctgcaccag caggttgccg agcccaccgg
    17881 caccgggcgc caccagcacc cgatccccgc tgcgcaccgg cgtccactcc agcagcgcga
    17941 ccgcggtgct gccgtcgtcc atgaccgcca gcgcggtgcg cgggtccagg ccgtccggca
    18001 cgcggaacgc cgcgtcgggc gcggaaatcg cggtgtccgc gtacccgccg ccgtacccgc
    18061 tgcccgctcg cgcgatcacc cgcgcgcccg cccagccatc gggcacctcc gcgccgaccg
    18121 cggtcaccgt gcccgcgacc gccccgcccg gtacgtacgg cggctcgact tcgaagacgt
    18181 cagggccgtg ccctgaccgg atcagcgcgt ccagccagcc gacgcccgcc gcttcgacgt
    18241 cgatccggaa ttcgcccgct ttcggctccg gggtcggcag ttcgacgagt tccagtacgt
    18301 cgggggcgcc gaatcgggtc gccgttatcg ctctcatacg gacagtctgg aacttgaacc
    18361 atggttgaag tcaaggcaag cggccgaagc aggtgcggag atcggcgaac gcgcaccagg
    18421 caggcctgcg tttcgggagg tagccgcctg gcaccccgtt cgcggcgatc gcggcccggt
    18481 aggtggccac cgcgtcggtc gggcgtcgcg tcagcgccgg atcggtcagc gcgtccactg
    18541 tgtacagtcc gaaccgggac cggtagctgc cccattcgta gttgtcggtg atgctccagt
    18601 agttgtaccc gatcacgcgc acaccgtcgg cgagcgcgcg ctgcacccag tagaggtggt
    18661 cgcgcaggtg atcggaccgg gtgtagccgt cggcgcgcgg tttcccgtcg tcggacggca
    18721 tcccgttctc cacgacgtaa agcggcaggc cggggaactt ccgctgatag gtgcgcaacg
    18781 cgtagtagat cccgtccggt tgcggggcga ccttccagaa ctcgtccttc gccgcatgga
    18841 tcacgctgag gttgtcgagg ctcgcgccgt agtagtagtc gatgccgacg aagtccagct
    18901 tgcccctcac tttgtccaca aaggacgaat cgtcgaacca ttgcagcggc ggcggtttga
    18961 ccgcgaggtt gctggtgacc atcgcgccgg ggtcgagccg gtggatcagg tcgtaaccgc
    19021 ggcggtgcgc ggcgaccagg ttgtccatca tggcaccgga ctggaacacg cccagcttcc
    19081 cgttgcgttc ctcgaaggtc cggtacaccg tcggttcgtt gaaggtgacc cacagaacgc
    19141 ccagcccggc ataccgccgc acgaccaggt cggtgaacgc gagccagctc tcgaccgtgc
    19201 cagggttcgt ccagccgccg cggtcggcga tccagccggg atgggtgaag tgcgtcaggg
    19261 tgatcatggg tgtcatgccc gcggccttga tctcccgcac gacgtcgtcg tagtacgcga
    19321 gcgcgtcctg gtcgagcgcg ccgggccggg gttcgatccg cggccattcg acgctcatgc
    19381 ggaacgtcgt gacgcccatc gacttggcca gcgcgatgtc ctccgcgtag cggtggcgga
    19441 agtcgacggc gttcgcgtag ggatccttca ccgagtccgc gcgaccgtcg acgtagcgcc
    19501 gccaattgct gtcgggcggc gagccctcag cctggaagcc cgccgtcgac acgccccagt
    19561 ggaacccggg aggcatccgc ggaatcggca ccgatgccgc ggccacgggt gcctgtgccg
    19621 ccgcggtgag caccgcgatc gcgatcgcgg ccgacttcca tcgcctcgcc atggctcccg
    19681 agtatgcaag ggtatccttg cagcgcgcaa gggtagcctt gcatccatgt cgggagagac
    19741 tcgcgaacgc atcctggacg aggcgctgaa gcagttcgcc gagcgcggtt tcgacggcac
    19801 caccatcagc tcgatcgaac ggcgcgttgg gctggctggc ggcacgggca gcttccaccg
    19861 gcacttcccg tcgaagcgcg ccgtgttcga cgccgcgatc gagcgcgagc tgacgcgcct
    19921 gcgcacgatc cggcccgagc gcgaaccgcg cgccgacgca ccgctcgaac gcaggctcga
    19981 aagcgatctg gaattcctgc gcgaagtccg ctcgctgatc gagatcctga tccgcgaacg
    20041 cgccaccatg ccgcatctcg tcgagcgggt cggcgaagtg ctcgtggacg gcggcctggc
    20101 ggggatcacc agcgatctct tcccagtcgg ctcgcacgag gatccggccg cggccgcgac
    20161 gatcatgctg tgcgcgaccg tcggctactt cctcaccggc gagttcttcg gcaccccgcc
    20221 cggcggcgtc gaccccgccc gatacgccgc cgcactagcc gcgatgctca gcccgcgagg
    20281 ccgcccgaga cgcgcaggtt ctggccggtg atccaccgcg aatccggccc agcgaggaac
    20341 gcgaccacgt cggcgatgtc ctcgggttgt ccgagccgtc ccaacgcggt ttgcgcggtc
    20401 acgtactcga cggcctgctc gaggtgatcc cccgcggcgg cgcgcatcat gtcggtctcg
    20461 gtcgcgccgg gtgaaacgcc gttgacggtg atgccgcggc tgccgagttc ccgtgctgcc
    20521 accgcggtga tcgcgtccag cgcggctttg ctcgcgaagt acagggacag accgggcgtc
    20581 gatcccgacg acgagaggtt cacgatccgc ccgccgtccc gcagccgctt caccgcggcc
    20641 tgcaggatga agaacgggcc cttggtgttg atcgcgatga cttcgtcgaa gtcggcttcc
    20701 gtggcgtcgg cgatgccgcc catcggattc gcggcggcgt tgttcaccag gatgtcgagc
    20761 ccgccgagcc gttcgtcgac cgcggcgaag agacgttcga tatcagcgag tttcccttgg
    20821 tgggcttgga ccgcgatggc ttctccgccg cccgcggcga ccacttcgtc agccgctgtc
    20881 tgattgcccg cgaaggtgaa caagaccttc gccccttcgc tcaccaaccg cagcacaacc
    20941 gctcgcccga tcccacgcga cccacccgta accaaggccg tcttgccagc caatgtcccc
    21001 atgtccgact cccagcagcc agtgcccacc cacaccgggc gagcctcatc accaagtcgg
    21061 agcaacgacc acgtcctcga cacacccacg aagaaatcga gaaagtcggc gcagggaccg
    21121 aaacgcgtgc ctactgctgg tccaatgcaa acgtgcccag cgctcgtcaa ggcgaggagc
    21181 tggcaccgtg ggggtgtggg ggctcggccc ccactacgag acaagacacg gggaaggtgg
    21241 cgcgcagcgc caccgaacac aattcggaag tggccagggc cgggatcgaa ccggcgacct
    21301 tccgcttttc aggcggacgc tcgtaccaac tgagctacct ggccgttccc agcggaagac
    21361 tcggaggtaa ccacgagatc cggtgctggt tgccgatgac cggaccacgt cggaacgcaa
    21421 ggagctcgca ccgtgggggt ccggggggct cggccccccg ggggtttcgg cgagtgctgg
    21481 tggtccggtg tcaagcggac acgactacgc caaaacgagc gaccctgacg ggactcgaac
    21541 ccgcgacctt cgccgtgaca gggcgacgcg ctaaccaact gcgccacagg gccttgctgt
    21601 tgtaactgta ctgcgtactc ccaacgggat tcgaacccgc gttgccgcct tgaaagggcg
    21661 gagtcctagg ccgctggacg atgggagccc ggtcggtttt tcggcgaacc gaccgaccgt
    21721 gctctcaggt ccccctggga gcgaagccaa gcatacggca cgccgtgatc gccctgcgaa
    21781 accggggtgg cctaagccga tctcgggtcc ttgagctgcg tcgatgatgt catcggggtg
    21841 gcgtgctgtc gaatgaatag ctcgcgaacg ggtgggcggg cggggcgtgc gggtgcggcc
    21901 gaacgggtgg tcgcgcgctg tgagcggaca ggtactcgct tgcctggcgc cgttcgaggg
    21961 cttgcgaggc ggacggcgtc ggtcgaggcg tcgcgaacga cggggaatgc ggctcgggct
    22021 ggggaagatc gactccgcgc gggaactggg aggcacgcgg ggagggtgga caacgatcaa
    22081 gcaaaacggt gttactgtgg gttttcgcca acctcgatcg gcgaaagaac ttccgacgta
    22141 ctgaaggtca cgcgggatag atgtttgccc agggcggtcg gcctcagtat gtttttccgt
    22201 gcgtggggac gggtgcgacc agggggtgca cccgtcccca cgtgcacgtt caccgctcag
    22261 cgctggacag gggcctgccc gtcgtcgtcc gggttcaggc ccagcatgtc gagcagctgc
    22321 gaacagtcct ccacgccgtt ggcgcccctg gccaccgcca ggcgcgccct gcgcacttga
    22381 tcgtccgaca cgcgctggct gtcgctactg ccgctccgct gggcgggcac gccgccgctc
    22441 atgtcgccgc cttcgtgccg caggaggaac tgccgcactt ccacagaccc gctcatcgct
    22501 cctccgtacg gctcaacaac aactttggcc gcgaggctac tgagcgcgcg aggccccaca
    22561 cagccccccg cagagtgaac
//
