LOCUS       NZ_CP059997            10825 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Amycolatopsis sp. CA-230715 chromosome, complete genome.
ACCESSION   NZ_CP059997
VERSION     NZ_CP059997.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis sp. CA-230715
  ORGANISM  Amycolatopsis sp. CA-230715
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 10363158)
  AUTHORS   Jorgensen,T.S., Gren,T., Oves-Costales,D., Ortiz-Lopez,F.J.,
            Carretero-Molina,D., Gonzalez,I., Blin,K., Genilloud,O. and Weber,T.
  TITLE     Complete Genome Sequence of Amycolatopsis sp. CA-230715, Encoding a
            35-Module Type I Polyketide Synthase
  JOURNAL   Microbiol Resour Announc 10 (38), e0080521 (2021)
   PUBMED   34553997
REFERENCE   2  (bases 1 to 10363158)
  AUTHORS   Joergensen,T.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2020) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-JUN-2019
            Assembly Method :: Flye v. 2.4.1-geb89c9e; Unicycler v. 0.4.8-beta;
            SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology :: Illumina MiSeq; PacBio RSII; Oxford
            Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 09:21:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,592
            CDSs (total)                      :: 9,498
            Genes (coding)                    :: 9,434
            CDSs (with protein)               :: 9,434
            Genes (RNA)                       :: 94
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 10 of 64
            Pseudo Genes (incomplete)         :: 60 of 64
            Pseudo Genes (internal stop)      :: 2 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:58:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 270825
            Orig. end                         :: 281650
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059997.1.
            Annotated using prokka_raw 1.14.6 from
            https://github.com/tseemann/prokka.
            Bacteria and source DNA available from Fundacion MEDINA, Grenada,
            Spain.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10825
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10825
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10825
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5825](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5825
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            814..1476
                     /locus_tag="HUW46_RS01255"
                     /old_locus_tag="HUW46_00252"
     CDS             814..1476
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068875.1"
                     /locus_tag="HUW46_RS01255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_00252"
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="WP_215545502.1"
                     /transl_table=11
                     /translation="MSRRRKLIVAAVLLVALVVAAFVLPVPSPARLRDWAAGAGPMTAV
                     LFFSAYSLLTVPPIPRTVFNLAAGLLLGNAVGIGVALAATGVSGALGFGLARLLGRDLV
                     TAHLHRTPVRVVNERLSGGGLLAVTSLRLIPVVPFAPLGYCCGISSIRFWPYLAGTVLG
                     SVPGTVAVVVLGDALTGGTPPALIACYGGFALLGGLGLLKVVRAKERAPEEVAAVTS"
     gene            1613..3166
                     /locus_tag="HUW46_RS01260"
                     /old_locus_tag="HUW46_00253"
     CDS             1613..3166
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658040.1"
                     /locus_tag="HUW46_RS01260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_00253"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_256451447.1"
                     /sec_met_domain="Pkinase (E-value: 3e-52, bitscore: 176.7,
                     seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGHYRLVEHIGSGAMGVVWRAVDERLERSVAVKQILAQPGLSES
                     ERATVRQRAMREAKNAARFQHPNAIVVFDIAEHGGDPCLVMEYLPSKSLSAVLAEQPTL
                     PLAQVARIGEQVASALVAAHRAGIVHRDIKPGNILIDESGTTKITDFGISRAAGDLTLT
                     QTGLIGGTPAYLPPELARGSDPAPASDVFSLGATLYHALEGQPPYGNSSNQLALLYTAA
                     NGQVIPPRQSGQATALLMSLLRAEADERPSMSEARERLAALAAGEPAPAATAVSPPLFG
                     PGRQPAAPVDTPGQGIPSAAAPPPWQRTPAAPATPPRLPTSTFAPAAVPTAAAPHRPPE
                     TAQAAKSAPNRKAIMLGSGAAGVLVVALIVFLVLNNSSKNEAQTPPPATPSSSSAKPPS
                     SSKSPSTAPSSPVGQTPSTGKVEYSQAGGLVDQFISAKGSAAAWGMLTPGAQGAFKGAE
                     DFAAYWAGMSSSYGVIRADRGSNADGSVDMYVNLPSGRKAFRVVNTPAGLKIDSDTHVG
                     "
     gene            3202..3498
                     /locus_tag="HUW46_RS01265"
                     /old_locus_tag="HUW46_00254"
     CDS             3202..3498
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983661.1"
                     /locus_tag="HUW46_RS01265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_00254"
                     /product="hypothetical protein"
                     /protein_id="WP_215545504.1"
                     /transl_table=11
                     /translation="MADTGRDPRRRFHDDLVGLDPNDPEARAFAEHLDRIERCEPAFTV
                     EASIDGVADFADSSARLGGLRYWAAVLLVALIMLGVIVASWEILVRAAHWLAE"
     gene            3521..3943
                     /gene="msrB"
                     /locus_tag="HUW46_RS01270"
                     /old_locus_tag="HUW46_00255"
     CDS             3521..3943
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333001.1"
                     /locus_tag="HUW46_RS01270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_00255"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_215549598.1"
                     /transl_table=11
                     /translation="MKPVVGATPRVVKSDQEWRQELAPEEYAVLRQAATERPFTGEYTD
                     TETVGAYECRACGAELFRSTTKFASHCGWPSFYDPADSDAVLLREDRTMGMKRIEVLCA
                     SCHSHLGHVFEGEGYDTPTDQRYCINSIALKLVPEG"
     gene            4040..5008
                     /locus_tag="HUW46_RS01275"
                     /old_locus_tag="HUW46_00256"
     CDS             4040..5008
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="HUW46_RS01275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_00256"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_215545505.1"
                     /transl_table=11
                     /translation="MDEPWGISGPGFLGLYAVLLLVPIVVKPLWVASLKRAGGGRQSSV
                     PDVYHLAYVAGGPDRVTDTAIAALVDSERLRVSSSGRLTDTGTIPTDSVEAAVVRHVAT
                     TGGTVRQVRLRMRVDNAMYAVEREVTESGLIVSDDKRRTVWRSVMGFYALVLVLGIARW
                     INGNSHGRSTGYLTWFVLAAVAALLLTRFLMRRDRPGLASSAGDKVLAEAREARQRAEN
                     AEQTEEAGAPGQRSPVSGLAGAGLFAGAVGAVALGGLAMYPDEELSDAMMPQAGGLFSG
                     NGSWGGGNYGGGSSCGGSSCGGGSSCGGGGSSCGGGGGCGG"
     gene            5001..5825
                     /locus_tag="HUW46_RS01280"
                     /old_locus_tag="HUW46_00257"
     CDS             5001..5825
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422374.1"
                     /locus_tag="HUW46_RS01280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_00257"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_254125689.1"
                     /sec_met_domain="DUF692 (E-value: 4.4e-82, bitscore: 274.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADSLAPLGVGIGWRPEIDLSIARLPGVDWVEVVAENLHVEHLPA
                     SLLSLKQRGLPVLPHAVSLSLGGAEDLDTARVEHLAAVTDALDAPMASDHVCFVRAGGL
                     DAGHLIPLPRTRAALDVLVDNVKLTQSILGVPFAVENIAAVLEWPDAELSEARFLAELT
                     ERTGCGLIIDVANLYANARNIGTDPEVFLDEIPLEKIAYVHVAGGVDRDGVYHDTHAHP
                     VRPEVLELLTELRSRTEPPGVLLERDDDYPSDTELAGELAAIRAALDAGARA"
     gene            5822..6157
                     /locus_tag="HUW46_RS01285"
                     /old_locus_tag="HUW46_00258"
     CDS             5822..6157
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033529.1"
                     /locus_tag="HUW46_RS01285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_00258"
                     /product="hypothetical protein"
                     /protein_id="WP_215545507.1"
                     /transl_table=11
                     /translation="MTSERRKLAEQQAALLEALLSGGPSPAGFDEDRVHVERKVLRAKR
                     RRVVGYLRPDLPDALGGRFAALFDTYADERPKFVGTRAREDAAAFAAWLVDRGELPKPR
                     RWNPFRR"
     gene            complement(6170..6862)
                     /gene="hemQ"
                     /locus_tag="HUW46_RS01290"
                     /old_locus_tag="HUW46_00259"
     CDS             complement(6170..6862)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /locus_tag="HUW46_RS01290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_00259"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_215545508.1"
                     /transl_table=11
                     /translation="MARLNYNELNDTIRYTAWSVFRVEPGKLPDDRGPAARETTEYLDA
                     LEGKGVIVRGVYDVAGLRADADYMIWWHAEEIEQVQAAYTGFRRTPLGKVSTPVWSQVA
                     LHRPAEFNKSHVPAFLAGEEARKYICVYPFVRSYEWYLLPDDERRKMLADHGKEARDYP
                     DVRANTVASFALGDYEWILAFEADELHRIVDLMRHLRGTEARLHVREEVPFYTGTRVPP
                     SELITNLP"
     misc_feature    6863..6876
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.63"
                     /tool="antismash"
     gene            complement(6889..8304)
                     /gene="hemG"
                     /locus_tag="HUW46_RS01295"
                     /old_locus_tag="HUW46_00260"
     CDS             complement(6889..8304)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671909.1"
                     /locus_tag="HUW46_RS01295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_00260"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_215545509.1"
                     /transl_table=11
                     /translation="MTGIAVVGGGISGLTAAYRLRAELGDRAEITVVESSPWLGGKLRT
                     IDLAGRAYDVGAEAFLARRPEAMDLVAEIGLGDRLTHPTGARATVLAGGHATGLPAGTV
                     MGVPSSPEAVKGVLSPEAVALVAAEAGLPPVELGEHDTDLGALLRRRFGDELVDRLVDP
                     LLGGVYAGGADGLGLRATMPGLAKAIDGGARSLTEAAASITPPSPSGAPVFGTLKGGLG
                     TVITRLAELSRARIRLGVPARALRRTATGWRLELGAAAQAHAPEQSVLDVDAVVLAVPA
                     PAARRLLADAVPAAAEAFAGIELASMAVVAMAFPRGTELPESSGLLIGAGERAPSGTPL
                     TAKAFTFSARKWGHYGENDGPVLLRGSVGRFGETASLQAGDDELADLVRRDLAETTGLT
                     TTPIEVAVTRWGGGLPQYSVGHFERVARIERAVAEAGGLAVTGAALHGVGIPACIATAT
                     AAATTVTAGMPTE"
     gene            complement(8301..9410)
                     /gene="hemE"
                     /locus_tag="HUW46_RS01300"
                     /old_locus_tag="HUW46_00261"
     CDS             complement(8301..9410)
                     /EC_number="4.1.1.37"
                     /GO_function="GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007027609.1"
                     /locus_tag="HUW46_RS01300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_00261"
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="WP_215545510.1"
                     /transl_table=11
                     /translation="MPTTPASPAPGPAVAQAAAPGSSPARRVLPDAPFLVAARGGTPAR
                     LPVWFMRQAGRSLPEYRALREGVAMLDSCFDPEMLAEITLQPVRRHGVDAAILFSDIVV
                     PLRAAGVDIDIVPGTGPVVASPVRSAADVAALPELDPAQVGAVADGVRLLVERLGTTPL
                     IGFAGAPFTLASYLIEGGPSRNHEHTKALMHSEPRVWHALAGKLADIALTFLMAQLDAG
                     ADAIQLFDSWAGALSPRDYREFVLPHSTRVFEGVAAYGVPRIHFGVGTGELLTAMRDAG
                     PDVVGVDWRVPLDEAVRRLSPSEKPVVVQGNLDPALLLGSWPVVEAEVRRIAEEGKAAA
                     GHVFNLGHGVLPGTDPDVLTRVVELIHQL"
     misc_feature    complement(8304..8306)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9499..10062
                     /locus_tag="HUW46_RS01305"
                     /old_locus_tag="HUW46_00262"
     CDS             9499..10062
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332994.1"
                     /locus_tag="HUW46_RS01305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_00262"
                     /product="DUF3000 domain-containing protein"
                     /protein_id="WP_215545511.1"
                     /transl_table=11
                     /translation="MTAMTHAPELFREAVAALQTVRPRPELTLESVRAPQRLAPWSYAL
                     SGETTGPADVLASGRLVLLHDPEGQENWNGVLRMVVYARAELDRELASDPFLPAVGWSW
                     LTDALEGAGAHLTALGGTVTETSSARFGDISGPARTDDLELRASWTPTDAELHSHGEAF
                     CQMMASMAGLPPVGVTLFEHRQSS"
     gene            10138..10785
                     /locus_tag="HUW46_RS01310"
                     /old_locus_tag="HUW46_00263"
     CDS             10138..10785
                     /EC_number="3.2.-.-"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /locus_tag="HUW46_RS01310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUW46_00263"
                     /product="type 1 glutamine amidotransferase family protein"
                     /protein_id="WP_215545512.1"
                     /transl_table=11
                     /translation="MATSYAYLYVTDTLADWEIGYLTAELDSGRYFAESGARMPVRTAG
                     LSLDPVTTKGGLRLVPDTTVDRITAEDAGVLILPGGDTWLEPVHDPVLAKAVELLEARV
                     PVAAVCGATMALANAGLLNERPHTSNNLGALQALCPGYEGEDHFRGEPAVTDGDLITAS
                     GVAPLHFAKAVLARLGVFSETALDAWFRLNAEQEESAYFELMGSLPQPATAH"
ORIGIN
        1 ggtcgacacg gtcgcgcgcg cggtgccgcg gaccgaccac gtcgaggcga cggtgcggtc
       61 gcgcgccgcc tggttgtagt cgaggaacac cttcccgccg cgttcctcct tccaccacgc
      121 gatcgtggcg cgctcggggg cacggcgctc gacctcgcgg cccagcgcga tcaccgcgtg
      181 ccgcaccgcg atgaaatccc attcggggcg gatcctcgcc agcacgtgca ccccgcggcc
      241 gcccgaggtc ttcgggtacc cggtgatccc cgcgtccgcc agcacctccc tgaccacgtg
      301 cgcgaccttc accgcgtctt cgaacccggc ggcgtcgggc gggtcgacgt cgatccgcag
      361 ttcgtcgggg tggtccacgt cggggcgccg taccggccac gggtggaagt cgaacgtgcc
      421 gaggttggcc gcccacgcga acaccgcggg ctcggtcggg cacacctcct ccgccttccg
      481 tcccgacggg aaggtgatct cgaccttgtc cacccagtcc ggcgcgcctt tgggcacgcg
      541 cttggcgtag aagaactctc cggcgacgcc gtcgggatac cgcttgagtg tggtcggccg
      601 ctcaccgagc gcgcgcacca tcggctcgcc gagcgcgatg tagtgctcga ccacgtgccg
      661 cttggtgatg ccgcgctccg ggaagtacgg cttgtccgga ctggacaccc gtaccgtgcg
      721 cgcgccgacc tggtattcga cggggtcacc gttcttcttg gccacgcgca cacggtagcg
      781 ggacgcgaag cccggtgccc taccgttgac cgcatgtccc gccggaggaa gctgatcgtc
      841 gcggcagtcc tgctcgtcgc gctcgtggtg gccgcgttcg tgctgccggt gcccagcccg
      901 gcgcggctgc gcgactgggc cgccggcgcg ggcccgatga cggcggtgct cttcttctcg
      961 gcctactcgc tgctcaccgt gccgccgata ccgcgcaccg tgttcaacct cgccgccggg
     1021 ctgctgctcg gaaacgccgt cggcatcggc gtcgcgctcg cggcgaccgg ggtgtccggc
     1081 gcgctcgggt tcggcctcgc caggctgctc ggccgcgatc tcgtcaccgc tcatctccac
     1141 cgcacaccgg tgcgcgtggt caacgaacgg ctctccggcg gcggcctgct cgcggtgacg
     1201 tcgctgcgcc tgatcccggt ggtcccgttc gcgccgctcg gctactgctg cggcatctcg
     1261 tcgatccggt tctggccgta cctcgcgggc accgtgctcg gcagcgtgcc gggcacggtc
     1321 gcggtggtcg tgctcggcga cgcgctcacc ggcggcaccc cgcccgcgct gatcgcctgc
     1381 tacggcggtt tcgcgctgct cggcgggctc ggcctgctca aggtcgtccg cgcgaaggag
     1441 cgggcgccgg aggaagtggc cgcggtcacg agctgacggg cgcggctgac cgcaggcgca
     1501 aactccggcc cgctacactc gaacgagtgc gcggcggcgt tgttcgcgct cggtcaagcc
     1561 acgaacgcga gatgtttcta caggagtagg cgtcatcgac accggtcagc tcatcgcagg
     1621 gcactatcgc ctcgtcgagc acattggcag cggtgccatg ggcgtcgtct ggcgtgctgt
     1681 cgacgaacga ctcgaaagat ccgtcgccgt caagcaaatc cttgcccagc cgggtctttc
     1741 cgagtcggaa cgggcgaccg tgcgccagcg cgccatgcgc gaggccaaga acgcggcgcg
     1801 gttccagcac cccaacgcga tcgtcgtctt cgacatcgcc gagcacggcg gcgatccgtg
     1861 cctggtcatg gaatacctgc cgtcgaagag cctgtcggcg gtcctcgccg agcagccgac
     1921 gctgccgctc gcccaggtcg cccgcatcgg cgagcaggtc gcctccgcgc tcgtcgcggc
     1981 gcaccgcgcc ggcatcgtgc accgggacat caagcccggc aacatcctca tcgacgagtc
     2041 cgggaccacg aagatcaccg acttcggcat ctcgcgcgcc gccggtgacc tcaccctcac
     2101 ccagaccggc ctgatcggcg ggacgcccgc gtacctgccg cccgaactgg cccgcggctc
     2161 cgatcccgcg cccgcgtccg acgtgttctc gctcggcgcc acgctgtacc acgcgctcga
     2221 aggccagcca ccgtacggca acagctccaa ccagctcgcg ctgctctaca ccgcggccaa
     2281 cgggcaggtc atccccccgc gccagtccgg ccaggcgacc gcgctgctga tgagcctgct
     2341 gcgcgccgag gccgacgaac ggccgagcat gagcgaagcg cgcgaacgcc tcgccgcgct
     2401 cgccgccggg gaaccggcgc ccgccgcgac agcggtgtca ccgccgctgt tcggaccggg
     2461 ccgccagccc gccgccccgg tcgacacgcc cggccaaggc atccccagcg ccgccgcgcc
     2521 cccgccgtgg cagcgcaccc ccgccgcgcc cgcgaccccg ccccggctgc cgacctcgac
     2581 gttcgcgccc gccgccgtgc ccaccgcggc ggcgccgcac cggccaccag agaccgcgca
     2641 ggcggcgaag agcgcgccca accgcaaggc gatcatgctc ggctccggcg ccgccggggt
     2701 gctcgtcgtc gcgctgatcg tgttcctggt gctgaacaac agcagcaaga acgaggcgca
     2761 gaccccgcct ccggccactc cgtcctcgtc ctcggccaaa ccgccgtcgt cgtcgaagtc
     2821 gccgagcacg gcaccgtcct cccccgtcgg ccagacgccc agcacgggca aggtcgagta
     2881 cagccaggcg ggcggcctag tcgaccagtt catcagcgcc aagggctccg ccgccgcatg
     2941 gggcatgctg accccgggcg cgcaaggggc cttcaagggg gcggaggact tcgccgccta
     3001 ctgggccggt atgtccagca gctacggggt aatccgcgcc gaccgtggct cgaacgcgga
     3061 cggctcggtc gacatgtacg tcaacctccc cagcggccgc aaggcgttcc gcgtggtcaa
     3121 cacaccagcc gggctgaaaa tcgactcgga cacccacgtc ggttgatgcg cgcgggcccg
     3181 ccggtggctt accgtgggcc catggccgat accgggcgtg acccccgtcg ccggttccac
     3241 gacgacctcg tcggcctcga cccgaacgat ccggaggcgc gcgcgttcgc cgagcacctc
     3301 gaccggatcg aacgctgcga gcccgctttc accgtcgaag cgtccatcga cggtgtcgcc
     3361 gacttcgccg attccagcgc ccgcctcggc ggcctgcggt actgggcggc ggtgctgctg
     3421 gtggcgctca tcatgctcgg cgtgatagtc gcctcgtggg agatcctggt gcgggccgcg
     3481 cactggctcg cggagtagcg tggggaccat gaaacccggc atgaagcctg ttgtcggcgc
     3541 gaccccccga gtcgtgaagt ccgaccagga atggcggcag gagctcgcgc cggaggagta
     3601 cgcggtgctg cgccaggccg ccaccgagcg tcccttcacc ggcgaataca ccgacaccga
     3661 gaccgtcggc gcgtacgaat gccgcgcctg cggagccgaa ctcttccgca gcaccaccaa
     3721 gttcgcgagc cactgcggct ggccgtcgtt ctacgacccc gccgactccg acgcggtgct
     3781 gctgcgcgaa gaccgcacga tgggcatgaa gcgcatcgag gtgctgtgcg cttcgtgcca
     3841 cagccacctc ggccacgtgt tcgagggcga gggctacgac acgccgaccg accagcgcta
     3901 ctgcatcaac tcgatcgcgc tgaaactggt gccggaaggc tgagcccgcc ggtctcgaac
     3961 tggtcactcc ccccgtgcgg cgtgcccgca cccactacgg ttggtcactc gcgagttcta
     4021 ccgagctggg ggagcgatca tggatgaacc gtggggtatt tccggtcccg gattcctggg
     4081 gctctacgcg gtcctgctgc tggtgccgat cgtggtgaaa ccgctgtggg tcgcgtcgct
     4141 caagcgcgcc ggtggcggca ggcagtcctc ggtgcccgac gtctaccacc tcgcctacgt
     4201 cgcgggcggg ccggacaggg tcaccgacac cgcgatcgcc gcgctggtgg acagcgagcg
     4261 cctgcgggtg tcctcgtccg gcaggctcac cgacaccggc acgatcccga cggacagcgt
     4321 cgaggccgcg gtggtgcggc acgtggccac caccggcggg acggtgcgcc aggtccggtt
     4381 gcggatgcgc gtggacaacg cgatgtacgc ggtcgaacgg gaggtcaccg agtccggcct
     4441 catcgtctcc gacgacaagc gccgcaccgt gtggcgttcg gtcatgggtt tctacgcgct
     4501 ggtcctcgtg ctcgggatcg cgcgctggat caacgggaac tcgcacgggc gctcgaccgg
     4561 gtacttgacc tggttcgtgc tcgccgcggt cgcggcgctg ctgctcaccc gcttcctgat
     4621 gcgacgcgac cgccccggcc tggccagctc ggcgggcgac aaggtgctgg ccgaggcacg
     4681 ggaagcgcgg cagcgggccg aaaacgcgga gcagaccgaa gaagccggag cacccggcca
     4741 gcgctcgccc gtgagcgggc tggccggcgc cggcctgttc gcgggcgcgg tcggcgcggt
     4801 ggcgctgggc gggctcgcga tgtatcccga cgaagagctc agcgacgcga tgatgcccca
     4861 ggcgggcggg ctgttcagcg gcaacggctc gtggggcggc ggcaactacg gtggcggctc
     4921 gtcgtgcggc gggtcttcct gcggcggcgg ttcttcgtgc ggtggcggcg gttcgtcctg
     4981 cggcggtggt ggcgggtgcg gtggctgact cgctcgcccc gctcggggtc gggatcggct
     5041 ggcggccgga gatcgacctg tcgatcgccc ggctgcccgg cgtcgactgg gtcgaggtcg
     5101 tcgccgagaa cctccacgtc gagcacctgc ccgcgtcgct gctttcgttg aagcaacgcg
     5161 gacttccggt gctgccgcac gcggtttcgc tgtcgctggg cggtgcggag gatctcgaca
     5221 ccgcccgcgt cgagcacctg gccgcggtca ccgacgcgct cgacgcgccg atggcgagcg
     5281 atcacgtgtg cttcgtgcgc gcgggcgggc tggacgcggg ccacctcatc ccgctccccc
     5341 gcacccgcgc ggcgctggac gtgctggtgg acaacgtgaa gctgacccag tccatcctgg
     5401 gcgtgccgtt cgcggtggag aacatcgcgg ccgtgctcga atggccggac gcggagctgt
     5461 ccgaggcgcg gttcctggcc gagctgaccg agcgcaccgg ctgcgggctg atcatcgacg
     5521 tggcgaacct ctacgccaac gcgcggaaca tcggcaccga tcccgaggtc ttcctcgacg
     5581 aaattccgct cgagaagatc gcctacgtcc acgtcgcggg cggcgtggac cgcgacggcg
     5641 tctaccacga cacccacgcg cacccggtgc ggcccgaggt gctcgagctg ctcaccgagc
     5701 tgcggtcgcg gaccgagccg ccgggcgtgc tgctggaacg cgacgacgac taccccagcg
     5761 acaccgaact ggcgggtgag ctggccgcga tccgggccgc gctcgacgcc ggggcgcggg
     5821 cgtgacgtcc gaacggagga aactcgccga gcagcaggcg gcgctgctcg aagcgctgct
     5881 ctcgggcggg ccgtccccag cggggttcga cgaggaccgc gtgcacgtcg agcggaaggt
     5941 gttgcgcgcc aagcgaaggc gggtcgtcgg ctacctcagg cccgatctcc ccgacgcgct
     6001 cggcggccgg ttcgcggcgc tgttcgacac ctacgccgac gagcggccga agttcgtcgg
     6061 caccagggcg cgcgaggacg cggccgcgtt cgcggcgtgg ctcgtcgacc gcggcgaact
     6121 gccgaaaccg cggcggtgga acccgttccg tcgctagtcc acaatggact tacgggaggt
     6181 tggtgatcag ctccgacggc ggcaccctgg tcccggtgta gaacgggacc tcctcgcgga
     6241 cgtgcagccg cgcctcggtg ccgcgcaggt ggcgcatcag gtcgacgatg cggtgcagtt
     6301 cgtcggcctc gaaggcgagg atccactcgt agtcgccgag cgcgaacgac gcgaccgtgt
     6361 tggcgcgcac gtccgggtag tcccgcgcct ccttgccgtg gtcggcgagc atcttgcggc
     6421 gctcgtcgtc cggcagcagg taccactcgt aggagcgcac gaacgggtag acgcagatgt
     6481 acttgcgcgc ctcttccccg gcgaggaagg cgggcacgtg gctcttgttg aactcggcgg
     6541 gccggtgcag cgcgacctgg ctccacaccg gcgtcgacac cttgccgagc ggggtgcggc
     6601 ggaacccggt gtaggcggcc tgcacctgct cgatctcctc ggcgtgccac cagatcatgt
     6661 agtcggcgtc ggcgcgcagc ccggccacgt cgtacacccc gcgcacgatc acgcccttgc
     6721 cctcgagcgc gtcgaggtac tcggtggtct cgcgcgcggc ggggccgcgg tcgtccggca
     6781 gcttgcccgg ctcgacgcgg aacaccgacc acgcggtgta gcggatggtg tcgttgagct
     6841 cgttgtagtt caggcgcgcc atggcaccca gtatgccaac ctgcggacct actcggtcgg
     6901 catccccgcc gtgacggtcg tcgccgccgc ggtggccgtc gcgatgcacg ccgggatgcc
     6961 gacgccgtgc aacgccgcgc cggtgaccgc gagcccgccc gcctcggcga ccgcgcgctc
     7021 gatgcgggcg acccgctcga agtgcccgac cgaatactgc gggaggccgc cgccccaccg
     7081 cgtcaccgcg acctcgatcg gtgtcgtggt cagccccgtg gtctcggcca gatcgcgccg
     7141 gacgagatcg gccagttcgt cgtcgccggc ctgcagcgac gccgtctcgc cgaaccggcc
     7201 gacggacccg cgcagcagca ccgggccgtc gttttccccg tagtggcccc atttcctggc
     7261 cgagaaggtg aacgccttcg cggtcagcgg ggtgccggac ggggcgcgct cccccgctcc
     7321 gatcagcagc cccgaggact cgggcagctc ggtcccgcgc gggaaggcca tcgcgacgac
     7381 ggccatcgac gccagctcga tcccggcgaa cgcctccgcc gcggcgggca cggcgtcggc
     7441 cagcagccgc ctcgcggcgg gcgcgggcac cgcgagcacg accgcgtcga cgtcgagcac
     7501 ggactgctcc ggcgcgtgcg cctgcgcggc cgcgcccagc tccaggcgcc acccggtcgc
     7561 cgtgcgccgg agcgcgcggg cggggacgcc gagccggatc cgagcgcgcg aaagctcggc
     7621 gagcctggtg atgacggtgc cgagtccgcc cttgagcgtc ccgaaaacgg gagccccgct
     7681 cggggacggc ggcgtgatcg aggccgcggc ttcggtgagc gaccgagcgc cgccgtcgat
     7741 cgccttcgcc agccccggca tcgtcgcgcg caggccgagc ccgtcggcgc cgcccgcgta
     7801 cacgccgccc agcagcggat cgacgagccg gtcgaccagc tcgtccccga accgccgccg
     7861 cagcagcgcg ccgagatcgg tgtcgtgctc gcccagctcg acgggcggca gcccggcttc
     7921 ggcggcgaca agggcgaccg cctccggcga gagcaccccc ttgaccgctt cgggcgagct
     7981 gggcaccccc atcaccgtcc cggcgggcag cccggtcgcg tgcccgcccg cgagcaccgt
     8041 ggcgcgggcg ccggtcggat gggtcagccg gtcgccgagg ccgatctccg ccaccaggtc
     8101 catcgcctcg ggcctgcgcg ccaggaaggc ctccgccccc acgtcgtagg cgcggcccgc
     8161 caggtcgatc gtgcggagct tgccgccgag ccacggcgac gattcgacga ccgtgatctc
     8221 cgcgcggtca ccgagttcgg cgcgcagccg gtaggcggcg gtgagcccgg agatcccgcc
     8281 gccgacgacg gcgataccgg tcataactgg tggatcagct cgacgaccct ggtcagcacg
     8341 tccgggtcgg taccggggag cacgccgtga ccgaggttga acacgtgccc cgcggcggct
     8401 ttgccctctt cggcgatgcg ccgcacctcg gcctcgacga ccggccacga ccccagcagc
     8461 agcgccggat cgagattgcc ctgcaccacc acgggtttct ccgacgggga aagcctgcgg
     8521 acggcctcgt cgagcggcac gcgccagtcg acgccgacga cgtccggccc cgcgtcgcgc
     8581 atcgcggtga gcagttcgcc cgtccccacg ccgaagtgga tccgcggcac gccgtaggcc
     8641 gcgaccccct cgaagacgcg cgtggaatgc ggcagcacga attcgcggta gtcccgcggt
     8701 gaaagcgcgc cagcccacga gtcgaagagc tggatcgcgt cggcaccggc gtcgagctgg
     8761 gccatcagga aggtcagcgc gatgtcggcg agcttgcccg cgagcgcgtg ccacacccgc
     8821 ggctcggagt gcatgagcgc cttggtgtgc tcgtggttgc ggctgggccc gccttcgatc
     8881 aggtaggagg cgagcgtgaa gggcgcgccc gcgaacccga tcagcggcgt cgtgccgagc
     8941 cgctcgacga gcaggcgcac gccgtcggcg accgcgccga cctgggcagg gtcgagttcc
     9001 ggcagcgccg cgacatcggc ggccgagcgg acgggcgagg ccacgacggg gccggtgccc
     9061 ggcacgatgt cgatgtcgac accggccgcg cgaagcggga ccacgatgtc gctgaacagg
     9121 atcgcggcgt cgaccccgtg cctgcggacc ggctgcaggg tgatctcggc gagcatctcg
     9181 gggtcgaagc acgagtcgag catcgccacg ccttcgcgca gcgcacggta ctcgggcagg
     9241 gaccggcccg cctggcgcat gaaccacacc ggcagcctcg ccggtgtgcc accgcgcgcg
     9301 gcgaccagga agggggcgtc gggcagcacg cggcgagcgg ggctcgagcc gggtgcggcg
     9361 gcctgcgcga cggccgggcc gggcgcgggt gaagcaggcg tggtaggcat cgcctcaatc
     9421 attccacgcc cggtttgccg cgtgtttccc cggcgcgtct gcccgaccac cgcgcccttc
     9481 ccgccttaca gtcgagcggt gaccgcgatg acgcatgcgc ccgagctctt ccgtgaagcg
     9541 gtcgcggcgc tgcaaaccgt ccggccccgg ccggagctga ccctcgaaag cgtccgcgcg
     9601 ccgcagcgcc tcgctccctg gtcctacgcg ctgagcggcg agaccaccgg ccccgccgac
     9661 gtgctggcct ccggcaggct ggtgctcctg cacgaccccg aaggccagga gaactggaac
     9721 ggcgtgctcc gcatggtcgt ctacgcccgt gccgaactgg accgcgaact cgccagcgac
     9781 cccttcctcc ccgcggtggg ctggtcgtgg ctgaccgacg cgctggaggg cgccggtgcg
     9841 cacctgaccg cgctgggcgg cacggtcacc gaaacctcgt ccgcccgctt cggcgacatc
     9901 tcgggaccgg cgcggaccga cgacctcgaa ctccgggcct cctggacgcc caccgacgcc
     9961 gaactgcact cccacggcga ggccttctgc cagatgatgg ccagcatggc ggggttgccg
    10021 ccggtcggcg tgaccctctt cgagcaccgg cagagctcct gaggctcgtt tcgtctgggg
    10081 gttttgtcgg tcccccgccg tagcgttcgc tccaaccgaa ccgggaggag cgccaccatg
    10141 gccacgtcct acgcctacct gtacgtcacc gacacgctcg ccgactggga gatcgggtac
    10201 ctgaccgccg agctcgacag cggccggtac ttcgccgagt ccggcgccag gatgccggtc
    10261 agaaccgcgg ggctgagcct cgacccggtc accaccaagg gcgggctccg cctcgtcccg
    10321 gacaccaccg tcgaccggat caccgccgag gacgccggtg tgctgatcct gcccggcggg
    10381 gacacctggc tcgaacccgt gcacgacccg gtgctggcca aggccgtcga actgctcgag
    10441 gcgcgcgttc cggtggccgc ggtctgcggg gcgacgatgg cgctggcgaa cgccgggctg
    10501 ctcaacgagc gcccgcacac cagtaacaac ctgggggccc tgcaggcact gtgccctggc
    10561 tacgagggtg aagatcactt tcgtggcgag cccgcggtca ccgacggtga cttgatcacg
    10621 gccagcggcg tggcaccgct gcacttcgcg aaggcggtgc tcgcgcgcct cggggtgttc
    10681 tccgagacgg cgctcgacgc ctggttccgg ttgaacgccg agcaagaaga atcggcctat
    10741 ttcgagctga tgggttcgct tccgcaaccg gccacggcac actaggcggg tgactcggaa
    10801 gcggggctcg cgcgtttgca cgcct
//
