LOCUS       NZ_CP076103            11260 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Lysobacter capsici strain NF87_2 chromosome, complete genome.
ACCESSION   NZ_CP076103
VERSION     NZ_CP076103.1
KEYWORDS    RefSeq.
SOURCE      Lysobacter capsici
  ORGANISM  Lysobacter capsici
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Lysobacter.
REFERENCE   1  (bases 1 to 6315468)
  AUTHORS   Liu,Y., Qiao,J., Liu,Y., Liang,X., Zhou,Y. and Liu,J.
  TITLE     Characterization of Lysobacter capsici strain NF87-2 and its
            biocontrol activities against phytopathogens. European Journal of
            Plant Pathology
  JOURNAL   Eur J Plant Pathol 155 (3), 859-869 (2019)
REFERENCE   2  (bases 1 to 6315468)
  AUTHORS   Liu,Y., Qiao,J. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) Institute of Plant Protection, Jiangsu
            Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing
            210014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: nextDenovo v. 2.0-beta.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018732085.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 13:41:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,303
            CDSs (total)                      :: 5,240
            Genes (coding)                    :: 5,174
            CDSs (with protein)               :: 5,174
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 16 of 66
            Pseudo Genes (incomplete)         :: 52 of 66
            Pseudo Genes (internal stop)      :: 2 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:02:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6207936
            Orig. end                         :: 6219196
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076103.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11260
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="17"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11260
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11260
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6260](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6260)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(134..148)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 20.47"
                     /tool="antismash"
     misc_feature    456..471
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.64"
                     /tool="antismash"
     gene            567..1130
                     /locus_tag="KME82_RS25980"
                     /old_locus_tag="KME82_25980"
     CDS             567..1130
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489588.1"
                     /locus_tag="KME82_RS25980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_25980"
                     /product="fasciclin domain-containing protein"
                     /protein_id="WP_215496613.1"
                     /transl_table=11
                     /translation="MNTRLKTLAVVVVLALSGAAFGADAKGMNNPMVGGAPMLVTRDIV
                     DNAVNSKDHTTLVAAVKAAGLVETLKGPGPFTVFAPTNAAFAALPAGTVDTLLKPENKA
                     TLTSVLTYHVVPGKLDGKALMAQAKAGGGRAQLKTVQGEVLTVRTDGGKVTVTDSKGNT
                     ANVTIANVYQKNGVIHVVDKVLMP"
     gene            complement(1425..2120)
                     /locus_tag="KME82_RS25985"
                     /old_locus_tag="KME82_25985"
     CDS             complement(1425..2120)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /locus_tag="KME82_RS25985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_25985"
                     /product="energy transducer TonB"
                     /protein_id="WP_215496614.1"
                     /transl_table=11
                     /translation="MHRFTRSFKVIAYSICIGLSAIAAPALAQDVEIGPDISGRVDWPY
                     KSEHPPAYPDGVADALCRSDATVLRVDVDEQGEVLEVKIEHSDGNAALDEAAVQAARGW
                     RYYPAVSQGKPHADSIRVPVEFKADDACWQALPDTTAHPDVESMGRFPPSWPAAVKTGP
                     GGVVVLKIKVDDTRKLAYAVEVDRSSGDDAIDNAAAVAANLWTFEPARLKGVPVRSVLR
                     WTVPYGTSR"
     gene            complement(2166..3404)
                     /locus_tag="KME82_RS25990"
                     /old_locus_tag="KME82_25990"
     CDS             complement(2166..3404)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /locus_tag="KME82_RS25990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_25990"
                     /product="GPI inositol-deacylase"
                     /protein_id="WP_215496615.1"
                     /transl_table=11
                     /translation="MPKRSPADAVIGHGTDLRGIGRLTIDAITGVTDLVESVHATIVAL
                     PSPLGAAVRDPTRGITGLVYRSVRGITRFVGDGLDLALAQFAPLLNRIDSLPQRGAVVS
                     ALNGVLGDHLAATDNPLAIDMQLRKDGRPLKLGRKSLAAAYPQATGKVVVLIHGLCMND
                     LQWNWNGHDHGAALARDAGWTPVYLHYNTGRHISSNGRELSAQLDKLLREWPLPVERFA
                     IVCHSMGGLVARSACHSAQRSKRQWLSRLDSLVFLGTPHHGAPLERAGSWFDMLIALSP
                     YTAPFARLGKIRSAGIQDLRHGNLIEADWRKHADDGRRDPRTPLPLPESVRSYAIAVST
                     DKEGASNVGGLAAAERFSGDTLVPVASALGLHPDPAFDLHIPKRHRWIGHGLHHLDLLG
                     DAQVYRRIRRWLG"
     misc_feature    3614..3629
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.77"
                     /tool="antismash"
     gene            3653..4885
                     /locus_tag="KME82_RS25995"
                     /old_locus_tag="KME82_25995"
     CDS             3653..4885
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /locus_tag="KME82_RS25995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_25995"
                     /product="WG repeat-containing protein"
                     /protein_id="WP_215496616.1"
                     /transl_table=11
                     /translation="MRSSLPRLIVFALLCASAGGCAPVRYYQALSGKQVAQRKPPPIVE
                     HGQPAPAHQNVATFHDGLHLARLHKQGEQTFGVVDRKSRVVIPIGYQHIIDRGADGFGA
                     IGLQSLRAFDRQGRQTAATLFEDELDFVNGQAVAVIKPPGGDSDAERYGVIDADGNTVI
                     PFEYHRVFERHTQSRPTVSWYLVQREVSAAGEPKRYRSGLLDARGRTIVPIEYFDISPN
                     VDIEGLDEGWAMLFEREHSSVAVNFLTGLRMPRTGGSFYGRTVGYSTLLNPEPETLNQY
                     EKMQYRKANPARYRLELFDTQGRLVYDQRDIRVTWDIKGLIAVSRAGKFALLDAQGRPL
                     SEFVYDAVKDFGGGQALMERDGRKICIDAADPAGTQRAARECRKATTLKHKNDRERAPS
                     GFPAVYPRFGG"
     gene            complement(5001..6260)
                     /locus_tag="KME82_RS26000"
                     /old_locus_tag="KME82_26000"
     CDS             complement(5001..6260)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="KME82_RS26000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_26000"
                     /product="YcaO-like family protein"
                     /protein_id="WP_215496617.1"
                     /sec_met_domain="YcaO (E-value: 1.2e-40, bitscore: 138.6,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIPLERERSLADADAVIRGYVRDQGWRMQIDVLGEGIASTVCTLY
                     DADDVALSNGFGKGEAAASLTGAMYEAVEHYYTRKPPADAPIALVRSQHFAQDPRYAGL
                     PFAAAFEANAQQQLACRNYRRLDEHGPGDDTVPVPLFMTYPAYLHNLVEGDDFDYNSAI
                     RFSSNSGVAIGASLEEAAVHAIGELVERDAWSLFLIAHYLGDPTSLGVIVDPQSLPDDV
                     ARIYRAVCGQLDREVWLIDVTTDLQFPAFIATTADILADEETFPCGYGASLYAAHAAMR
                     ALTELVQCIDIKSRTPKMREYSRLALDTLSEYRKLRDCAYFQVRADRLERVDWAHPARA
                     AMPPKAMLADMIAHLRRHGIGVYYAINQHAPGAFCIVSCVSFELERFFMVTSGIAMGPG
                     RRGMDLLEGRLRVPALVPAV"
     gene            complement(6369..6506)
                     /locus_tag="KME82_RS26005"
                     /old_locus_tag="KME82_26005"
     CDS             complement(6369..6506)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KME82_RS26005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KME82_26005"
                     /product="hypothetical protein"
                     /protein_id="WP_157754304.1"
                     /transl_table=11
                     /translation="MKKAIDIPVFKPRPLIEDASKVISEKQIKVELTAKEKSFLDQYAK
                     "
     misc_feature    6633..6646
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.55"
                     /tool="antismash"
     misc_feature    complement(6696..6708)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     gene            complement(6735..8900)
                     /gene="pabB"
                     /locus_tag="KME82_RS26010"
                     /old_locus_tag="KME82_26010"
     CDS             complement(6735..8900)
                     /EC_number="2.6.1.85"
                     /GO_function="GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA]"
                     /GO_process="GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pabB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1018: isochorismate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /locus_tag="KME82_RS26010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_26010"
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="WP_215496618.1"
                     /transl_table=11
                     /translation="MRCLLIDNYDSFTWNLADQIGRAFGRAPTVLRNDACGWEQLRADE
                     RYDCIVVSPGPGSVEHEADFHVARQAIAHSALPLLGVCLGFQGIAYAEGGRIVHAPQPY
                     HGRVSRLHHDGSELFAGLPASFEVVRYHSLIAAAPLPPALQATAHTDDGLIMALRHRRR
                     PQWGVQFHPESILSEHGIDLIANFRDLVYRHHGRAFVSLIATTTTPPPAAEPPQALQLW
                     CKPLDAKLDPETVFNALYAQRRNSFWLDSQDAVEERAACSFMGAVDDGDLLAYRIADDD
                     AALSTGHALLDRIERELQAQVADPIPADGASAPAESALPFDFRGGFIGWLGYEMKALFD
                     GEGNGDSSGNAATRLPDALWMRVHRFLAFDHRRARAWAIAIAAPHTAQATDAQRWLETV
                     AAQAASTLAQPAPESAPSPAPLDTLEVDMDLGHDDYLAAIEHCRRAIVDGESYQICLTN
                     HFRFRAQLDPLALYLRLRRGNAAPFGAYLRSGDHHVLSTSPERFLRVDRGGRIQTKPIK
                     GTMRRSDDPRVDAQYARHLAESVKDRAENLMIVDLMRNDLNRVAVHATVEVPKLREVES
                     YRTVHQLVSTVEAQLRPDAGLIDLLRATFPGGSISGAPKRRTLQIIDRLERSARGVYCG
                     SIGYLGYNRVADLNIGIRTLSYDGDSVAFGAGGAITWLSDPQAEFQEVLLKAEAVLRPL
                     WGFLAEPDAGFAYELDGDRLRLRRVAR"
     gene            complement(9004..9723)
                     /locus_tag="KME82_RS26015"
                     /old_locus_tag="KME82_26015"
     CDS             complement(9004..9723)
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1060: 4'-phosphopantetheinyl transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /locus_tag="KME82_RS26015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_26015"
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="WP_215496619.1"
                     /transl_table=11
                     /translation="MRPDHDAFLHDAAPFDAQAQGQPLTGYACRFRLDAYDDALYPAHG
                     LQLPPQLARAVAKRRGEFLAGRICARRALARLGIASADIAIGPDREPLWPAGAIASISH
                     AGDRAVCLASADPRMIGLGIDIESRIDARLAGEIRGVVVDAAEQAVIEAGFADAVAGLA
                     AAFSAKEALYKALYPQVRAFFGFEAMRLAQVGDDRLRFLAAADLSPTVRAGQAFEVDIA
                     FEDGGVRSIACCLRARA"
ORIGIN
        1 gcgctggaaa gcctgcgcca gcgcgacgaa tacctcgaac gcatcgcggt gcgggtcgac
       61 gagcatgtga tcttcgtggc ggtcgacgac atcgtctgga tcaaggccaa ccgcaacacc
      121 gtgcagatcc acctggccga tcgcatccac gaactgcgcg agaccctgtc cacgctcgcc
      181 gcgcgcctgg acccgcgccg cttcgcccgc gtgcatcgct cggcgatcgt caacgtgcag
      241 cgggtgcgca ccatccatcc gtggttcaac ggccaccatg tcgtgaccat ggacaacggc
      301 cagcaactgc gcatgagccg gtatcagcac gagacctttc tgcgcctggt cggttcgcgg
      361 cgcgaggact gaacagcgcg cgacgcgcgt tcagcgcttc atcaccgtta gttaacgcat
      421 cgctcgcgcc gccgatgttc attcatcgac gcgatgcgat cgcctgttcg aacgcatccg
      481 tatccaggtt cgacccgtag ttcctcatgc aaacgatccg gccgatacgt tgcgatgcat
      541 ccaatcattc aatgaggaac gtatccatga acacccgact caagaccctc gcggtcgtcg
      601 tagtgctggc gctgtccggc gcggcgttcg gcgccgatgc caagggcatg aacaatccga
      661 tggtcggcgg cgcgccgatg ctggtgactc gcgacatcgt cgacaacgcg gtcaactcca
      721 aggatcacac caccctggtc gccgcggtca aggcggcggg tctggtcgaa acgctcaagg
      781 gccccggccc gttcaccgtg ttcgcgccga ccaatgccgc cttcgccgcg ttgccggccg
      841 gtacggtcga cacgctgctc aaacccgaaa acaaggcgac cttgaccagc gtgctgacct
      901 accacgtggt gcccggcaag ctcgacggca aggcgctgat ggcgcaggcc aaggccggcg
      961 gcggacgcgc tcagctcaag accgtgcagg gcgaagtgct gacggtcagg accgacggcg
     1021 gcaaggtcac ggtgaccgac agcaagggca acacggccaa tgtcaccatc gccaacgtgt
     1081 atcagaagaa cggcgtgatc catgtcgtcg acaaggtgtt gatgccgtaa gtcgtcgcag
     1141 gcaggcaagc ggccggcgtt gtgcgccggt tgtttgccgg cgcgcatatc ggcgtgttcg
     1201 gcgtacttcg ccgaagcgtc gcaggcgagg tgtcgcggtc gcggcttgcg ccgctcctac
     1261 agtcggatta cgtaggctgc gccaaggtcg tgctcggact tcgtcgccac gaatgccccc
     1321 tgtaggagcg gcgcaagccg cgaccgcgac acttcaatcg ccaacgcatg tcatcgaaag
     1381 ctgtcgacgc ggcaggtatc cgcgaaaaaa cgcccgcgac cgcgtcagcg cgacgttcca
     1441 tagggaaccg tccagcgcaa caccgaccgc accggcacgc ctttcaaccg tgcaggctcg
     1501 aaagtccaca agttcgcggc cactgcggcc gcgttgtcga tggcgtcgtc gccgctggag
     1561 cgatcgacct cgacggcgta ggcaagcttg cgcgtgtcgt cgaccttgat cttcaacacc
     1621 accaccccgc ccggaccggt cttgaccgcg gccggccagc tcggcggaaa gcgccccatc
     1681 gactcgacgt cgggatgcgc ggtcgtatcg ggcaaggcct gccagcacgc gtcgtccgcc
     1741 ttgaactcga ccggcacgcg gatcgaatcg gcatgcggct tgccttggct cacggccggg
     1801 taatagcgcc atccgcgcgc ggcctgcacg gccgcttcgt ccagcgccgc gttgccgtcg
     1861 ctgtgttcga tcttgacttc aagcacctcg ccctgttcgt ccacatcgac ccgcaggacc
     1921 gtggcgtcgc tgcggcacag cgcgtcggcg acgccgtcgg gataggccgg cgggtgttcg
     1981 ctcttgtacg gccagtccac gcgtcccgat atgtcggggc cgatttcgac gtcctgggcc
     2041 agtgccggcg cggcgatcgc ggacaggccg atgcagatcg agtacgcgat aaccttgaag
     2101 ctccgggtga aacgatgcat gccgtgtcct ttcgatgaag catccatgtg catgccgcca
     2161 gccgctcaac ccagccagcg gcgaatgcgc cggtacacct gcgcatcgcc gagcaggtcg
     2221 agatggtgca gaccgtggcc gatccagcga tggcgcttgg gaatgtgcag atcgaacgcg
     2281 ggatcgggat gcagccccag cgcgctcgcg accggcacca gggtgtcgcc gctgaagcgc
     2341 tccgccgcgg cgagcccgcc gacgttcgac gcgccctcct tgtcggtcga caccgcgatc
     2401 gcgtagctgc gcaccgattc gggcagcggc aacggcgtgc gcggatcgcg gcggccgtcg
     2461 tcggcgtgct tgcgccagtc ggcttcgatc agattgccgt ggcgcaggtc ctggatgccg
     2521 gcgctgcgga tcttgcccag ccgcgcgaac ggcgcggtgt acgggctcag cgcgatcagc
     2581 atgtcgaacc agctgccggc gcgctccagc ggcgcgccgt gatgcggcgt accgaggaac
     2641 accaggctgt cgagccggct cagccactgg cgcttgctgc gctgcgcgct gtggcaggcg
     2701 ctgcgcgcga ccagtccgcc catgctgtgg cagacgatcg cgaaacgctc caccggcagc
     2761 ggccactcgc gcagcagctt gtcgagctgc gccgacagct cgcggccgtt gctggagatg
     2821 tggcggccgg tgttgtaatg caggtacacc ggcgtccagc cggcatcgcg ggcgagcgcg
     2881 gcgccgtggt cgtggccgtt ccagttccac tgcaggtcgt tcatgcacag gccgtggatc
     2941 agcacgacca ccttgccggt ggcctgcgga tacgccgccg ccagggattt tcgcccgagt
     3001 ttgagcggcc ggccgtcctt gcgcagctgc atgtcgatcg ccagcggatt gtcggtcgcg
     3061 gccaggtgat cgccgagcac gccgttgagc gccgacacca ccgcgccgcg ctgcggcagc
     3121 gaatcgatcc ggttcaacaa cggcgcgaac tgggccaacg ccagatccag gccgtcgccg
     3181 acgaagcggg tgatgccgcg cacgctgcgg tagaccaggc cggtgatgcc gcgcgtgggg
     3241 tcgcgcaccg ccgcgccgag cggcgacggc agggcgacga tggtggcgtg gacggattcg
     3301 accagatcgg tgacgccggt gatggcgtcg atggtcaggc ggccgatgcc gcgcaggtcg
     3361 gtgccgtggc cgatcacggc gtcggcgggc gagcgtttcg gcatgggcat cctgcgcggg
     3421 ctgagcggac gcccagccta ctgcacgcga agcgcattgg cgcgcgcacc aggccgcatc
     3481 gccggtcgcg gccgcgatgg agcgcgccat cggcgcctgc ggaccggtcc gtcgcgcagc
     3541 gactcgccgt cggcgaggca cgccgacgcc gacgcggtac gcgtctggtt cgccgatacc
     3601 gcgaccgccg attcatgcta atcatcacct tgatcccccg tgccggaccc cgatgcgcag
     3661 cagcctgccc cgcctcatcg ttttcgcctt gctgtgcgcc tcggccggcg gctgcgcgcc
     3721 ggtgcgctac taccaggcgt tgtccggcaa gcaggttgcg cagcgcaagc caccgccgat
     3781 cgtcgagcac gggcagcccg cgcccgcgca tcagaacgtc gcgacgttcc acgacggctt
     3841 gcacctggcg cggctgcaca agcagggcga gcagaccttc ggtgtcgtcg accggaaaag
     3901 ccgggtggtg atcccgatcg gctatcaaca catcatcgat cgcggcgccg acggtttcgg
     3961 cgcgatcggc ctgcaaagcc tgcgcgcatt cgaccggcag ggccggcaaa cggcggccac
     4021 gctgttcgaa gacgaactgg acttcgtcaa cggccaggcg gtcgcggtga tcaagccgcc
     4081 cggcggcgat tccgatgcgg agcgttacgg ggtcatcgat gccgatggca acaccgtgat
     4141 cccgttcgag taccaccgcg tcttcgaacg ccacacccaa tcgcggccca cggtgtcgtg
     4201 gtacctggtg caacgcgagg tcagcgcggc gggtgagccg aagcggtacc gcagcggctt
     4261 gctggacgcc cgggggcgaa cgatcgtgcc gatcgaatac ttcgatattt ccccgaacgt
     4321 ggatatcgaa ggcctcgacg aaggctgggc gatgctgttc gaacgcgagc acagcagcgt
     4381 tgcggtcaac tttctcaccg gcctgcgcat gccgcgcacg ggcggctcgt tctacggccg
     4441 caccgtgggc tattcgacct tgctcaatcc agagccggaa acgctcaacc agtacgagaa
     4501 gatgcaatac cgcaaggcca atccggcgcg ttatcggctg gagctgttcg acacccaggg
     4561 ccggttggtc tacgaccagc gcgatatcag ggtgacctgg gacatcaaag gcctgatcgc
     4621 agtgtcgcgc gcaggcaagt ttgcgctgct cgatgcgcag ggccggccgc tgagcgagtt
     4681 cgtgtacgac gcggtcaagg acttcggcgg cggccaggcg ctgatggaac gcgacggcag
     4741 aaaaatctgc atcgatgcgg ccgacccggc cggcacccag cgcgccgcgc gcgaatgccg
     4801 caaggccacc acgttgaagc acaagaacga tcgcgagcgc gcgcccagcg ggttccccgc
     4861 cgtctacccg cgtttcggcg gctgaaacgc ggggcagcac cgacggtgcg ccgaacgcga
     4921 cgccgccttc aagtcgatgg ccttatctca tcggcgtttc gaatcatcag cctcgagtcg
     4981 caggatcccg atcgcggcat tcaaaccgcc ggcaccaacg ccggcacccg caggcgccct
     5041 tccagcaagt ccatgccgcg ccggcccggg cccatcgcga tgcccgaggt caccatgaag
     5101 aaacgttcga gttcgaacga cacgcagctg acgatgcaga acgcgcccgg cgcgtgctgg
     5161 ttgatcgcgt agtacacgcc gatgccgtgg cggcgcaggt gcgcgatcat gtcggcgagc
     5221 atcgccttgg gcggcatcgc cgcgcgcgcc ggatgggccc agtcgacccg ttcgagccga
     5281 tcggcacgga cctggaagta cgcgcagtcg cgcagcttgc ggtactcgct cagcgtatcc
     5341 agcgcgagcc ggctgtactc gcgcatcttc ggcgtgcgcg attttatgtc gatgcattgc
     5401 accagttcgg tcagcgcgcg catcgcggcg tgcgcggcgt acagcgacgc gccgtagccg
     5461 cagggaaagg tttcctcgtc ggcgaggatg tcggcggtgg tggcgatgaa ggccgggaac
     5521 tgcaggtcgg tggtcacgtc gatcagccac acctcgcgat cgagttgccc gcacaccgcg
     5581 cggtagatgc gcgcgacgtc gtcgggcagc gactgcggat cgacgatcac gcccaggctg
     5641 gtcgggtcgc ccaggtaatg cgcgatcagg aacagcgacc aggcgtcgcg ctcgaccagt
     5701 tcgccgatcg cgtggaccgc ggcctcctcc aggctggcgc cgatcgccac gccgctgttg
     5761 gagctgaagc ggatcgcgct gttgtagtcg aaatcgtcgc cttcgaccag attgtgcagg
     5821 tacgccggat aggtcatgaa cagcggcacc ggcacggtgt cgtcgccggg accgtgttcg
     5881 tcgagacggc ggtaattgcg acacgccagc tgttgctgcg cattggcctc gaacgcggcc
     5941 gcgaacggca ggccggcgta acgcgggtcc tgcgcgaagt gctgcgaacg caccagcgcg
     6001 atgggcgcat cggccggcgg cttgcgggtg tagtagtgct cgaccgcttc gtacatcgcg
     6061 ccggtcagcg acgcggcggc ctcgcccttg ccgaagccgt tgctgagcgc gacgtcgtcg
     6121 gcgtcgtaca aggtgcatac ggtcgaggcg atgccttcgc cgagcacgtc gatctgcatg
     6181 cgccagccct gatcgcgcac gtagccgcgg atcaccgcat cggcgtcggc cagcgatcgt
     6241 tcgcgctcca ggggaatcat ggcctcgctc cttgccttgc tttgtctcgt cctgcctggc
     6301 gcgctcgccc gcagcgcggc ggcacgaacg ggtccgtgcc gcgcgcccga gagcaaaccg
     6361 tcgccggctt acttcgcgta ctggtccagg aaggattttt ccttggcggt cagctcgacc
     6421 ttgatctgct tctcgctgat gaccttgctg gcgtcttcga tgagcggacg cggcttgaac
     6481 acggggatat cgatcgcctt tttcatggtg tccctcttca ggtgatgtcg ccgatggcgt
     6541 ggatgcagcg acgccgaagc cacgaccttg cgacttccgt gtcgactgcc ctttttctat
     6601 acccatggtg taagaacggt gtcaatcacg caggcatcga cattgcctgt gtggaaccgg
     6661 gatcacgtcc acatgcgcgt acgcgcggcg gatcggtcca aattgcaatt gcatgaacgc
     6721 gccgatggcg cggctcagcg cgcgacccgg cgaaggcgca ggcggtcgcc gtccagctca
     6781 taggcgaagc cggcgtcggg ctcggccagg aacccccaca gcggccgcag caccgcttcg
     6841 gccttgagca gcacttcctg gaattcggcc tgcgggtccg acagccaggt gatcgcgccg
     6901 ccggcgccga aggcgacgct gtcgccgtcg taggacaggg tgcggatgcc gatgttgaga
     6961 tcggccacgc ggttgtagcc caagtagccg atcgaaccgc agtagacgcc gcgcgcgctg
     7021 cgttcgagcc ggtcgatgat ctgcagggtg cggcgcttgg gcgcgccgct gatcgaaccg
     7081 ccggggaagg tcgcgcgcaa caggtcgatc aggccggcgt cgggacgcag ctgcgcctcg
     7141 accgtgctga ccagctgatg cacggtgcga tagctttcga cttcgcgcag cttcggcacc
     7201 tcgaccgtgg catgcaccgc gacccggttg aggtcgttgc gcatcaggtc gacgatcatc
     7261 aggttttcgg cgcggtcctt gaccgattcg gccagatgcc gcgcgtactg cgcatcgacc
     7321 cgcgggtcgt cggagcggcg catcgtgccc ttgatcggct tggtctggat gcggccgccg
     7381 cggtccacgc gcaggaagcg ttccggtgag gtgctcagca cgtgatggtc gccgctgcgc
     7441 aggtaggcgc cgaacggcgc cgcgttgccg cgacgcaggc gcaggtacag cgccagcgga
     7501 tcgagttgcg cgcggaagcg gaagtggttg gtcagacaga tctggtagga ctcgccgtcg
     7561 acgatggcgc ggcggcagtg ctcgatcgcg gccaggtaat cgtcgtggcc caggtccatg
     7621 tcgacttcga gcgtatccag cggcgcgggc gacggcgccg attccggcgc gggctgggcg
     7681 agggtcgatg ccgcctgcgc ggcgaccgtt tcgagccagc gctgcgcgtc ggtcgcctgc
     7741 gcggtgtgcg gcgcggcgat cgcgatcgcc caggcgcgcg cgcggcgatg atcgaaggcg
     7801 aggaagcggt gcacgcgcat ccacagcgca tcgggcagcc gcgtggcggc gttgccgctg
     7861 ctgtcgccgt tgccttcgcc atcgaacagc gccttcattt cgtagcccag ccagccgatg
     7921 aagccgccgc ggaaatcgaa tggcagtgcg ctttcggccg gggcggacgc gccgtcggcc
     7981 gggatcggat cggcgacctg cgcctggagt tcgcgttcga tgcgatcgag cagcgcgtgc
     8041 ccggtcgaca gcgccgcgtc gtcgtcggcg atgcgatagg ccagcagatc gccgtcgtcg
     8101 accgcgccca tgaacgaaca cgccgcgcgc tcctcgaccg cgtcctggct gtcgagccag
     8161 aaactgttgc gccgttgcgc gtacagcgcg ttgaacacgg tttccggatc gagcttcgca
     8221 tccaggggct tgcaccacag ttgcaaggct tgcggcggct cggcggccgg cggcggtgtc
     8281 gtcgtcgttg cgatcaggct gacgaaggcc cggccgtggt gccgatacac gagatcgcgg
     8341 aaattcgcga tcaggtcgat gccgtgttcg ctcaggatcg actcgggatg gaactgcacg
     8401 ccccattgcg ggcgacggcg atgacgcagc gccatgatca ggccgtcgtc ggtgtgcgcg
     8461 gtcgcctgca acgccggcgg caacggcgcg gccgcgatca gcgagtgata gcgcaccacc
     8521 tcgaacgatg cgggcaaacc cgcgaacaat tcgctgccgt catgatgcag gcgcgaaaca
     8581 cggccgtgat acggctgcgg cgcatgcacg atgcggccgc cttcggcgta ggcgatgccc
     8641 tggaagccca ggcagactcc gagcaaaggc agcgcgctgt gcgcgatcgc ctggcgcgcg
     8701 acatggaaat cggcctcgtg ctcgacgctg cccggcccgg gcgacaccac gatgcagtcg
     8761 tagcgttcgt cggcccgcag ttgctcccag ccgcaagcgt cgttgcgcag cacggtcggc
     8821 gcgcggccga acgcgcgccc gatctggtcg gcgaggttcc aggtgaagga gtcgtagttg
     8881 tcgatcaaca ggcaacgcat gatccggcca ttgtcgtaca aagcccgggc gcggcggcgg
     8941 tttggcgatg gttcgcggcg attcggccag gcgcgcggcc gtcgactccg ccgcgaacgg
     9001 cgctcaggcg cgcgcgcgca ggcagcaggc gatcgaacgc acgccgccgt cttcgaaggc
     9061 gatgtcgacc tcgaaggcct gcccggcccg caccgtcggc gacagatcgg ccgcggctag
     9121 gaaacgcaga cggtcgtcgc cgacctgcgc cagccgcatc gcctcgaagc cgaagaacgc
     9181 ccgcacctgc gggtacagcg ccttgtacaa ggcttccttg gccgaaaacg ccgcggccag
     9241 tccggccacc gcgtcggcga agccggcttc gatcaccgcc tgctcggccg cgtcgaccac
     9301 cacgccgcgg atctccccgg ccaggcgcgc gtcgatccgc gattcgatgt cgatgcccag
     9361 gccgatcatg cgcggatcgg cgctggccag gcagaccgcg cggtcgccgg catgcgagat
     9421 cgatgcgatc gcgccggccg gccacagcgg ttcgcgatcc gggccgatcg cgatatcggc
     9481 cgaggcgatc cccaaccgcg ccagcgcgcg ccgcgcgcag atccggccgg cgaggaattc
     9541 gccgcgccgc ttggccaccg cgcgcgccaa ctgcggcggc agttgcaacc catgagcggg
     9601 gtacagcgcg tcgtcgtagg cgtcgaggcg gaagcggcag gcgtaaccgg tcagcggctg
     9661 gccctgggcc tgcgcgtcga agggcgcggc gtcgtggagg aaggcgtcgt ggtccgggcg
     9721 catgcgctca ggccttggca tcgcgcggcg gcgcggcggt gatacggtgg gcgggcgggt
     9781 gcgggcgacg cggtgaacgc atggggggtc cgttacgacg gtaggggcag tgtggggcgt
     9841 catcgcggat agatcgctgg aaacgaagcc gctgccttgg ctcatggtat cggctcgcga
     9901 cgcgcaacgg cccgccgaag cgggccgttg caaggcgatg cggcatcgcg ccgctacgca
     9961 ctcaggtgaa acgacgctcg gtgacgcggc agtcagttga cgctggccgc gctccacgcc
    10021 cgcgcgatcg tggtctgctc cggcgaaccg gccccgtaca gctcggccgc ggcggccagg
    10081 gtcgccgcgc gcgcctgcgg gtaggtcgtg ctggaggtga acttggtggt cagcgcgcga
    10141 taccagatct tgccgaactt gtcgcggccg atgccgacga tgccggtgtc gccgttgcag
    10201 accagttggg ccggcgtcag cgtggtcccg gccggggtca ccgcgccttc catcgccaga
    10261 taggccatgc ggttggcgac gccggaggtg tagtgcgggt cgtgaatccc gcccgccgcc
    10321 aggttcggat cgaaaccgct gccgccgatg tagcagttga acgacttgcc gtcggcgccc
    10381 tggttgtaca tgtcgcggaa cgccaagccg ccggtgcgca tgatttcgcc gatgcggtag
    10441 tcgcccggat cgttggggtt gttggcgtag aactcgacca gggtgccgag gatgtcggaa
    10501 ttggcttcgt tcaagccgcc ggcgtcgccc gagtaggcca ggttcgcggt cgccgcgttg
    10561 accccgtgcg acatctcgtg gccggccaca tcgatgccga cgatcgggcc atagccgcgg
    10621 ctggcgtcgc cgtcgccgta gaacatcacc ttggtcaggg cgaaataggc cgcgttgacg
    10681 ccggtggtgc cgccggtgga ggtcttaaaa ttggtgtggg cgtagctgct gatgcccttg
    10741 ttgtcgttgt acaggcccag gcggccatgg acgttcttgt agtagtccca ggtcgcgccg
    10801 acgccgtagt ggatgtcggt ggcgacggtg gcgcgatcgc tgagggtgtt gttgccccag
    10861 gtgttgtcgg tgtcggtgaa caaggtcgcg cccagggtcg cgccgaggat gtcgaacagg
    10921 ttggagatcg ccgcgccctt ggcgtcgtag accttgccgt tgccgcgggt ggtgtcgatc
    10981 aggtcgaact tggtcgcgct cttctggtcg gtcggcaggc tcaggtcgcc gtagtagaac
    11041 gacttgccgg tgccgatcgc ggccgcggtc tgcagctggt cctgcacgtc gagcacgcgg
    11101 ccatcggcgg cattgacgta gtaggtgacg aagccggagt ggcggtcggt ctcgtcgccc
    11161 tgcacggtga cctcgtaggc gagcaccggc gtggcgccgc gcgcatacac caccagttcg
    11221 ttgccgtgga tcttggcgac ccggccgttg aagcgcgcgc
//
