LOCUS       NZ_CP076103            11272 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Lysobacter capsici strain NF87_2 chromosome, complete genome.
ACCESSION   NZ_CP076103
VERSION     NZ_CP076103.1
KEYWORDS    RefSeq.
SOURCE      Lysobacter capsici
  ORGANISM  Lysobacter capsici
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Lysobacter.
REFERENCE   1  (bases 1 to 6315468)
  AUTHORS   Liu,Y., Qiao,J., Liu,Y., Liang,X., Zhou,Y. and Liu,J.
  TITLE     Characterization of Lysobacter capsici strain NF87-2 and its
            biocontrol activities against phytopathogens. European Journal of
            Plant Pathology
  JOURNAL   Eur J Plant Pathol 155 (3), 859-869 (2019)
REFERENCE   2  (bases 1 to 6315468)
  AUTHORS   Liu,Y., Qiao,J. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) Institute of Plant Protection, Jiangsu
            Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing
            210014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: nextDenovo v. 2.0-beta.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018732085.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 13:41:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,303
            CDSs (total)                      :: 5,240
            Genes (coding)                    :: 5,174
            CDSs (with protein)               :: 5,174
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 16 of 66
            Pseudo Genes (incomplete)         :: 52 of 66
            Pseudo Genes (internal stop)      :: 2 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:02:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5143023
            Orig. end                         :: 5154295
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076103.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11272
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="11"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11272
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11272
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6272](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6272)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            825..2489
                     /locus_tag="KME82_RS21465"
                     /old_locus_tag="KME82_21465"
     CDS             825..2489
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081388.1"
                     /locus_tag="KME82_RS21465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_21465"
                     /product="alkaline phosphatase"
                     /protein_id="WP_215495806.1"
                     /transl_table=11
                     /translation="MRQIPHSFAATVLGAAVLTALVALPLLATDAFAAPKQVARKAPPR
                     AESAQRWFEEGAQTARDGANLRPTPGKARNVILFVGDGMGISTIAAARIREGQLKGGSG
                     EENSLSFERLPYVSLSKTYSVDGQTPDSAPTMTAMVTGIKTNQGVLSVTQKAKYGDCAS
                     ARGQGVVSMLELSEALGLATGIVSTARITHATPAATYSHTPNRDWESDAELSAEAKANG
                     CKDIARQLIEFPYGDGLEVALGGGRSYFLPNTVSDPEDAGAAGRRKDGRNLPTEWAARP
                     GSSYVWNKAQFDAIDPRRTRHLLGLFERSHMEYEQDRPNDAGKEPSLADMTAKAIDVLE
                     NSSRKGYFLMVEGGRVDHAHHAGNASRALTDAIALSDAVRKALEKTSERDTLIIVTADH
                     SHTFTVAGYPDRGNDILGKVVTNGQLALDKNGKPYTTLGYVNGPGYRGPNARPDLTAID
                     TAHPDYLQEATVPLGDETHAAEDVAIYARGPGAHAFQGVVEQNTIFHVMGQSQRNTSIF
                     LCTLLGQCGQGRTRSAIPAPLMEDDLRAGMAKQSASL"
     gene            2489..3280
                     /locus_tag="KME82_RS21470"
                     /old_locus_tag="KME82_21470"
     CDS             2489..3280
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KME82_RS21470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KME82_21470"
                     /product="hypothetical protein"
                     /protein_id="WP_215495807.1"
                     /transl_table=11
                     /translation="MRAWVFMGTATLYVAGSLAFAGPVWAGDAVRSEAPMAGAIAGHRG
                     SNTSVAAKAASTPTAANAASTPALLAEYQVRDAHGERSLVLVRSADRIEYRMQGEPVEV
                     WRKTPDGIARLELFAKEQRSVSWSPGDLRTIGEMPQWEQLASPINPELRMRLQRHGEVK
                     AFGGVALRYRGESAEGQAIALEWIEGDGLPSYYRTGAHKGKSGEAGAYELRLRKLERVG
                     AEGAFTATSDYRETDYADLGDMELDPFAANYLKHRGQAHAH"
     gene            complement(4328..4870)
                     /locus_tag="KME82_RS21475"
                     /old_locus_tag="KME82_21475"
     CDS             complement(4328..4870)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KME82_RS21475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KME82_21475"
                     /product="hypothetical protein"
                     /protein_id="WP_215495808.1"
                     /transl_table=11
                     /translation="MKAIVVATISTLALLFAACSQFANARAAGMDGERRQPMPHDTRTP
                     ADPARIQALASDRFPDTFGGLYIEQRPRYRVVVNFSRDAQASLRQLTDDASFEAVAVTH
                     SLRDLERIGETVNQVVRAKGLDCTLSVDPKRPSLKVWLRDPDVAPARAALDEAGLARPF
                     VVVVAYERVEPTGVGRR"
     misc_feature    complement(4445..4447)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    4843..4863
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.8"
                     /tool="antismash"
     gene            complement(5001..6272)
                     /locus_tag="KME82_RS21480"
                     /old_locus_tag="KME82_21480"
     CDS             complement(5001..6272)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="KME82_RS21480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_21480"
                     /product="YcaO-like family protein"
                     /protein_id="WP_215495809.1"
                     /sec_met_domain="YcaO (E-value: 8.6e-43, bitscore: 145.6,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIPLERERSLAQAQQVIEAFLARQGWSAHIDSTDTGLQGAVCVLH
                     DADGEAIGSGFGKGEREAARVGALYEAVEHVYANASAPQERAELRLASSVFHDSSYVGM
                     PFHAEFGRQGKRRRRRQRKLSCLWYRSFFHDDYELAVPAFLCRPNALADLLPGDNFDYS
                     SVIRYGSNSGTAIGASLEEAAVHALGELVERDSWSLFLVAHYLGDPASFGAWIDPASMP
                     EAVAAVHAAASAQLGREVLLIDATSDLGVPAFIATTDRMVEGEVVYPGGYGASLYAEHA
                     AIRALTELVQTVVVGQRLTSVGDYSRQVLLALSEYPKLRACAYFQVDRERMRHAAWSHP
                     QRERQPPEALLRELVDTLEMGGVPVYHRVHHREGDAFCVVSCVALELERFLLVTTGMVM
                     APGRRGMALLEGTLMGRKRQNYVA"
     misc_feature    6291..6304
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.6"
                     /tool="antismash"
     misc_feature    complement(6297..6306)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: medium, score: 17.13"
                     /tool="antismash"
     gene            complement(6352..6489)
                     /locus_tag="KME82_RS21485"
                     /old_locus_tag="KME82_21485"
     CDS             complement(6352..6489)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KME82_RS21485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KME82_21485"
                     /product="hypothetical protein"
                     /protein_id="WP_215495810.1"
                     /transl_table=11
                     /translation="MKKLIGTTIVKNAVTLAIAAQQQVSQNQAPAPTPEEKAILDLLAK
                     "
     gene            complement(6687..7160)
                     /locus_tag="KME82_RS21490"
                     /old_locus_tag="KME82_21490"
     CDS             complement(6687..7160)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KME82_RS21490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KME82_21490"
                     /product="hypothetical protein"
                     /protein_id="WP_215495811.1"
                     /transl_table=11
                     /translation="MITRSLLSALLLSSFVAMPAFAQGAAEPSASPPAAASAQATVPKL
                     HIVWDCGECEHNDKVPPLIEQAYAEEAQKHSVTVSETEIAEVAITDIRQRPPGVRVMFG
                     VMAGRDRLALRIRYRDRDHEVKDTSSNIIQGLNSLSASVGRKAYAQLSAAPAP"
     gene            complement(7256..7933)
                     /locus_tag="KME82_RS21495"
                     /old_locus_tag="KME82_21495"
     CDS             complement(7256..7933)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /locus_tag="KME82_RS21495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_21495"
                     /product="energy transducer TonB"
                     /protein_id="WP_215495812.1"
                     /transl_table=11
                     /translation="MDRAGEGESLQARASSRRGERPNAVRVSAIAGALALHAWLLLAVT
                     LPVNPFERSAYVPAGDPLVIQIPYPEIPDPPGCGGLRFPARPPNLTAIAQHVRVSIDPT
                     PTLPLSVPSLLDTLRLPPVHYRASVDRPLQAVASPAPVYPRKALRAGLSGSVEIEVVVG
                     IDGVPLSARIARSSGHHALDEAALAAVLSGWRFQPRMRDGEAVEAVARVPIDFVLPGRV
                     ARG"
     misc_feature    7948..7963
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.6"
                     /tool="antismash"
     gene            8027..8692
                     /locus_tag="KME82_RS21500"
                     /old_locus_tag="KME82_21500"
     CDS             8027..8692
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KME82_RS21500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KME82_21500"
                     /product="hypothetical protein"
                     /protein_id="WP_215495813.1"
                     /transl_table=11
                     /translation="MAPTPNPPRIVALSELADAYRVPDYLPAHWRILVVPGDLRVDGDL
                     QLEWDRPDGKANPPRHDWRDAAGCPPDEAIDGVAIEGSLHVGGAIISHWAGPVLLVREH
                     LRARSLIAGGAFIRIRGDADVTDTVLGHTNHGELYIDGTATAPLLIWDDHFFNAATINA
                     PYTFDTRAAAWEDWEYDDDLDGYPVPEQLKPVLRDEFGCWDEVMDALRAGKDVVRRDP"
     gene            complement(8697..9809)
                     /locus_tag="KME82_RS21505"
                     /old_locus_tag="KME82_21505"
     CDS             complement(8697..9809)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF042736.2"
                     /locus_tag="KME82_RS21505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_21505"
                     /product="XVIPCD domain-containing protein"
                     /protein_id="WP_215495814.1"
                     /transl_table=11
                     /translation="MTDEERKRHEENTQFLARNAEFLDLPQEFQSLVLKSSHASADFKE
                     FFSNGGRLVADPSEPLAAYRSEPHREILVNKSQYEFAKTPQGAHLVAGLFSRMAHEVGH
                     DKDRHAVFPVNASADDYVQFRSEKEARAIFNAFPIFADLSKSEPSFRPRWSDLGYSTLG
                     LDWPQLYDGWKDGSLDDASVIKAMAKVVVDQPYTRADGLEDQNQDGRLTQRDLYLRDYQ
                     RLLRPSPSQTDASVDPSDPADRALIRQLHDKLPGALAGQNSMPAGQALDCIAANLAVQC
                     RLAGIRHPDHVVLGSPISDGRRNLFAVEGGLSDPAHLRAHVPADPAMQAPVRESVQQLD
                     ALSKERAQEQAQSQSQAPVQQQSGSPVIKL"
     misc_feature    complement(9303..9305)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(9627..9629)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9886..10437
                     /locus_tag="KME82_RS21510"
                     /old_locus_tag="KME82_21510"
     CDS             9886..10437
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KME82_RS21510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KME82_21510"
                     /product="hypothetical protein"
                     /protein_id="WP_215495815.1"
                     /transl_table=11
                     /translation="MATKLYITSIRLTFGWLAFATVIAGCTSAPSIGTTPSASSLSIEQ
                     LVRLPAENGHAGLDQVVRALQARYGERSNVELSPLTNTATPITLADGTVITQAVTNING
                     SDDVARDVFLGIAERPCLDAHRIAAMIGAKKGYEVDGDDMTTGHSSYFFQSAQIDIEME
                     AQRQGPACVRSIAIYKRLSQ"
     gene            10735..11217
                     /locus_tag="KME82_RS21515"
                     /old_locus_tag="KME82_21515"
     CDS             10735..11217
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KME82_RS21515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KME82_21515"
                     /product="hypothetical protein"
                     /protein_id="WP_215495816.1"
                     /transl_table=11
                     /translation="MFRISALAVFLVSCNTHKTKSASDALDTQSLIRVPAQRGAAGVDQ
                     VTSALQARYGVASGTPLSPFVNSKAPVSLDDGAVIVQTVTNPDARDVRVDLAAKPCLPV
                     ARAAEWIGAKRMLATAGSDIVTGHVDYRFDSAEVRIDLTGEYGGEECLRVIEIYKQ"
ORIGIN
        1 ccagtactgc gcctcgccgt aaccggtggc ctgcagcacg ccggtcacgc cggcatgggt
       61 gaactgcacc aggcaatggg cgtcgccgct gcacgaattg agttcgggca ggtcctcgca
      121 gatctggcac aggcccggat cggccgcgca gattttttcg tacttgccgc cgaccacgtt
      181 ggccttgcac ttcggatcga ccaacggctg ccagccctgc gccagcacgg ccttgcggaa
      241 gtcgccgtag gccatgccct tgctcagcgc cgcttgcgtg ttcttgacga tcgtcgccga
      301 gtccgcggcc gcaggcgcag cgctcggcgc ggcggccggc atcgcggcgg cttgcgcatt
      361 cgcatcggac gcgggtgcag gcgcggcctc ggccggcgcg gtggcggcgg gcgccggcgc
      421 ggcggtggcc gcggccgctt cggatgccgc attaccggaa ttgcatgccg ccatgacgac
      481 gcacaacgcc aacgcactca gactgtgaac aagtttcacg caacctcccg attcgaaccc
      541 atggacggcc gtgtcgtccc gccgtgccgt cggcgaccgc ggggcttcca agccttgtct
      601 ggcgatgcgc cagtgttcct gccggaacct gccgataata gcgacagcgt acccactgcg
      661 gcgaaaccat cagcgacgca acaagctgaa cagcggcacc gcccatcggc gaacgccgca
      721 aacgcgaact gcgcctcttt ttcggccgat ccgggccctc gcgtcatttg catgcaacgc
      781 acgctcggca cagtgcagcc tcccccgatt tacccgagcc tcgcatgcgc cagattccgc
      841 attctttcgc cgccaccgtc ctcggcgcgg ccgtcctcac cgcgctcgtc gccctgcccc
      901 tgctggccac cgacgccttc gccgcgccca agcaggtcgc acgcaaagcg ccgccgcgcg
      961 ccgagagcgc gcagcgctgg ttcgaggaag gcgcgcagac cgcgcgcgac ggcgccaacc
     1021 tgcgcccgac cccgggcaag gccaggaacg tcatcctgtt cgtcggcgac ggcatgggca
     1081 tttccaccat cgccgccgcg cgcatccgcg aaggccagct caagggcggc agcggcgaag
     1141 agaattcgct gtcgttcgaa cgcctgccgt acgtgagcct gtccaagacc tactcggtcg
     1201 acggccagac cccggactcg gcgccgacca tgaccgcgat ggtcaccggc atcaagacca
     1261 accagggcgt gctcagcgtc acccagaaag ccaagtacgg cgactgcgcc tccgcgcgcg
     1321 gccagggcgt ggtgagcatg ctggaactgt ccgaagcgct cggcctggcc accggcatcg
     1381 tcagcaccgc gcgcatcacc cacgccaccc cggccgcgac ctactcgcac acgccgaacc
     1441 gcgactggga aagcgacgcg gaactgtccg ccgaagccaa ggccaacggc tgcaaggaca
     1501 tcgcccgcca gctgatcgaa ttcccctacg gcgacggcct ggaagtcgcg ctcggcggcg
     1561 gccgcagcta cttcctgccc aacaccgtga gcgatcccga agacgccggc gccgccggcc
     1621 gccgcaagga cggccgcaac ctgccgaccg aatgggccgc gcgcccgggt tcgtcgtacg
     1681 tgtggaacaa ggcccagttc gatgcgatcg acccgcgccg cacccgccac ctgctcggcc
     1741 tgttcgagcg ctcgcacatg gaatacgaac aggaccgtcc caacgacgcc ggcaaggaac
     1801 ccagcctggc cgacatgacc gccaaggcca tcgacgtgct ggaaaacagc agccgcaagg
     1861 gttacttcct gatggtcgaa ggcggccgcg tcgatcacgc ccaccacgcc ggcaacgcca
     1921 gccgcgcgct gaccgacgcc atcgcgctgt cggacgcggt gcgcaaggcg ctggagaaaa
     1981 cctccgagcg cgacaccctg atcatcgtca ccgccgacca cagccacacc ttcaccgtcg
     2041 ccggctaccc cgatcgcggc aacgacatcc tcggcaaggt cgtcaccaac ggccagctcg
     2101 cgctggacaa gaacggcaag ccgtatacga cgctcggcta cgtcaacggc cccggctacc
     2161 gcggcccgaa cgcacgcccg gacctgaccg cgatcgacac cgcgcacccg gattacctgc
     2221 aggaagccac cgtgccgctg ggcgatgaaa cccacgccgc cgaagacgtc gccatctacg
     2281 cccgcggccc cggcgcccac gcgttccagg gcgtggtcga gcagaacacg atcttccacg
     2341 tgatggggca gtcgcagcgc aacaccagca tcttcctgtg caccttgctc ggccagtgcg
     2401 gccagggccg cacccgctcg gcgattcccg cgccgctgat ggaagacgac ctgcgcgcgg
     2461 gcatggcgaa gcagtcggct tcgctgtaat gcgcgcctgg gtttttatgg gtacggcgac
     2521 gctgtacgtc gccggttcgc tcgcgttcgc cggtccggtg tgggctggcg atgcggtgcg
     2581 gtccgaggcg ccgatggctg gcgcgatcgc tggccatcgc ggcagcaaca cctcagtcgc
     2641 cgcgaaagcc gcatcgacgc caaccgctgc caacgcggca tccacgccgg ccctgctcgc
     2701 ggagtaccaa gtgcgcgacg cccacggcga acgcagcttg gtgttggtgc gctccgccga
     2761 tcgtatcgag taccgcatgc agggtgaacc ggtcgaggtc tggcgcaaga cgcccgacgg
     2821 gatcgcccgt ttggagttgt tcgcgaagga gcagcgcagc gtgtcgtggt cgcccggcga
     2881 tctgcgcacc atcggcgaga tgccgcagtg ggagcagttg gccagcccga tcaatcccga
     2941 gctgcgcatg cggctgcaac gtcatggcga ggtaaaggcc tttggcggcg ttgctttgcg
     3001 gtatcgcggc gaaagcgccg agggtcaggc gatcgcgctg gagtggatcg agggcgacgg
     3061 cctgccttcg tactaccgta ctggcgcgca caagggcaag tcgggtgagg ctggggctta
     3121 cgagttgcgg ttgcgcaagc tcgagcgggt cggcgccgag ggtgcgttta cggcgacttc
     3181 ggactatcgc gagacggatt acgcggatct gggcgatatg gagcttgatc cgtttgccgc
     3241 gaactatctg aagcatcgtg gtcaggcgca tgcgcactga ggttttcggc ggtgtgggtg
     3301 cgtttgtttt gtgatctggc ttagtgaagc tttgaggctt tgaagctttg aagctttgaa
     3361 gccagaggcc agaggcagga tcaacagctt tcgtccgcaa gcggccgagt tactttcttt
     3421 tgtctaaagc gacaaaagaa aggtaaccaa agaaaaacgc tttttcttga atcaagggcc
     3481 cgcaagtgcg gagcggacgc gggcacgcgc cacacgggac atccatgtcc cggtggcgcg
     3541 cgacgcgcat ccatgcgcgt cgccctccgg gtgtgctgtt gctaaggcga gttgatgact
     3601 tcgcagcgct ggatgaactg cgtggattgg ggcagaagcc acatggccgc cctgtaggag
     3661 cggcgcaagc cgcgaccgcg ccactacgac caccgcgccg gtcgcaacat cccctcgcct
     3721 tccgttacgg ccacagcctc gcaccgtcga acgacaaaaa tcgaacgtca gccaatgcaa
     3781 cgccacctcc ctgtaggagc ggcgcaagcc gcgaccgcgc cactacgacc accgcaccgg
     3841 tcgcaacatc ccctcacctt cctttacgac cacagcgtcg caccgtcgaa cgataaaaat
     3901 agaacgtcag ccaatgcaac gccacctccc tgtaggagcg gcgcaagccg cgaccgcgcc
     3961 actacgacca ccgcgccggt cgcaacatcc ccctcgcctt ccgttacggc cacaacctcg
     4021 caccgtcgaa cggcaaaaat cgaacgtcag ccaatgcaac gccacctccc tgtaggagcg
     4081 gcgcaagccg cgaccgcgcc actgcaacaa cgacgccggt tgcggcattg ccgtaatcgc
     4141 gcggtcgcgg catatggccg aaacagatcg ccgtggccgg cctcatctac cggtgccaag
     4201 actcaaatac agataccgcg caatacccga cggctcctca tgcgtccacc gggcgaacgt
     4261 ttgactccgc ccaggcctcg cacccgcccc gccatcggtg cctgtcgaag cgaaccgacc
     4321 accgcgctca acgccgccca acacccgtcg gctccacgcg ctcgtaagcc accacgacca
     4381 cgaacggccg cgccagcccc gcttcgtcca acgccgcccg agccggcgcc acgtctggat
     4441 cacgtaacca gaccttcaga ctcggccgct tcggatcgac ggacagcgtg cagtccagcc
     4501 ccttcgcccg cactacctgg ttgacggtct cgccgatccg ctccaggtca cgcagcgaat
     4561 gggtcacggc gacagcctcg aagcttgcgt catccgtcaa ctgacgcaag ctcgcctgcg
     4621 cgtcgcgact gaaattgacg accacccgat aacgcggccg ctgttcgatg tacaagccgc
     4681 cgaacgtgtc cggaaaccgg tcgctcgcca atgcctgaat ccgcgccggg tcggccggtg
     4741 tcctggtgtc gtgcggcatc ggttgccgcc gctcgccgtc catgccggcg gcccgcgcgt
     4801 tggcgaactg ggagcacgct gcaaaaagca aagcgagcgt cgagatcgtc gccacgacga
     4861 tggctttcat gagcagttcc tttcagtcaa tcaaggcgcc cgcgccgcgc attcggccga
     4921 ttccagacat caagacccaa cggtccaggc gcaggcgcgg ggtgatcggc atgacgaacg
     4981 cccgcgggcc ggcccgggga tcacgccacg taattctgcc gcttccgccc catcagcgtc
     5041 ccctccagca acgccatccc gcgccgcccc ggcgccatca ccatgccggt ggtgaccaaa
     5101 agaaaccgtt cgagctccaa cgccacgcag ctgaccacgc agaacgcatc gccttcgcga
     5161 tgatggaccc ggtgatacac ggggacgcct cccatctcca aggtatcgac caactcgcgc
     5221 aacaacgcct ccggcggctg gcgttcgcgt tgcggatgcg accaggccgc atgccgcatt
     5281 cgctcgcgat ccacctgaaa gtacgcgcag gcgcgcagct tgggatactc ggacaaggcc
     5341 agcagcacct gccggctgta gtcgccgacg ctggtcaggc gctgtcccac caccacggtc
     5401 tgcaccagtt cggtcagcgc gcggatcgcc gcatgctcgg cgtacaaaga cgcgccgtac
     5461 ccaccgggat acacgacttc gccctcgacc atgcgatcgg tggtggcgat gaaggccggc
     5521 acgcccagat cgctggtcgc atcgatcagc agcacctcgc gcccgagctg cgcgctcgcc
     5581 gccgcatgca cggccgcgac cgcttcgggc atcgaggctg ggtcgatcca ggcgccgaag
     5641 ctcgcggggt cgcccaagta atgcgcgacc agaaacaagg accacgaatc gcgctcgacc
     5701 aactcgccga gcgcatgcac cgcggcctct tccaggctcg cgccgatcgc cgtgccgctg
     5761 ttggaaccgt agcggatcac gctgctgtaa tcgaaattgt cgcccggcaa caaatccgcc
     5821 agcgcgttcg gccgacacag gaaggccggc accgcgagct cgtaatcgtc gtggaagaac
     5881 gagcgatacc acagacaact cagcttgcgc tggcgcctgc gcctgcgctt gccctgccgc
     5941 ccgaactcgg cgtgaaacgg catcccgaca taagacgaat cgtggaacac cgacgaggcc
     6001 aagcgcaact cggcgcgctc ctgcggcgcg ctggcattcg catacacatg ctcgaccgct
     6061 tcgtacagcg cgccgacccg cgctgcttcg cgctcgccct tgccgaagcc gctgccgatg
     6121 gcctcgccat cggcgtcgtg cagcacgcac accgcgcctt gcaggcccgt gtcggtgctg
     6181 tcgatatgcg cgctccaccc ttggcgcgcg agaaatgctt cgatcacctg ctgcgcctgc
     6241 gccagcgatc gctcgcgttc caacggaatc atgcccgcac tccttgcctc atggtttcaa
     6301 aaaacgaagc ggcccggccg gagccgtgcc gcttcggtcg taccccgccg cttacttggc
     6361 gagcagatcc aggatcgcct tctcttccgg cgtcggtgcc ggcgcctggt tctggctgac
     6421 ctgctgctgc gcggcgatgg ccagggtgac ggcgttcttg acgatggtcg ttccgatcaa
     6481 cttcttcata cagtccctcg taagtgttga tgcgtgcgat aactccccgc agcgccggcg
     6541 caaggcggcg gtgacgcgcg cccgcggcgg cctgccggtc agcaatacgc ccgccgtggc
     6601 cggtgcgtca atcgtggccc ggcaggggac gacgcgattg tgcgactgca accacagatt
     6661 cgcggcgtcc cggcgcggcc aggacgttac ggcgcaggcg ccgccgacaa ctgcgcatag
     6721 gccttgcggc cgaccgacgc cgacaggctg ttgagcccct ggatgatgtt ggacgaggtg
     6781 tccttgacct cgtggtctcg atcgcgataa cggatgcgca gcgccagacg atcgcgcccc
     6841 gccatcaccc cgaacatcac ccgcacgccg ggcggacgct ggcggatatc ggtgatcgcc
     6901 acttcggcga tctcggtttc ggacaccgtg accgaatgct tctgcgcctc ttccgcatag
     6961 gcctgctcga tcagcggcgg caccttgtcg ttgtgctcgc actcgccgca atcccagacg
     7021 atgtgcagct tgggcacggt cgcctgtgcc gacgcggcgg ccggcggcga cgcagacggc
     7081 tcggctgcac cctgcgcaaa tgcaggcatg gccacgaacg aagacagcaa cagcgccgac
     7141 aacaaactac gtgtaatcac acatccctcc tggagtgttt ttggttttcg atcgttgttc
     7201 ggcgaggcga acgcaaaccg cgctccctcg ccgacgcggc atcatgcccg atggttcaac
     7261 cgcgcgcgac gcgtccgggc aacacgaaat cgatcggcac ccgcgcgacc gcctccaccg
     7321 cctcgccatc gcgcatccgc ggctggaaac gccagccgct cagcaccgcg gccaacgccg
     7381 cctcgtcgag cgcgtggtgg ccactgctgc gcgcgatcct cgccgacaac ggcacgccgt
     7441 cgatgccgac gacgacttcg atctcgaccg agccgctgag tccggcccgc aacgccttac
     7501 gcggatacac cggcgcgggc gaagcgacgg cctgcagcgg cctatcgaca ctggcgcggt
     7561 aatgcaccgg cggcagtcgc aaggtgtcca gcaggctcgg caccgacaac ggcagcgtcg
     7621 gcgtgggatc gatcgacacg cgcacatgct gcgcgatggc cgtcaggttc ggcggccgag
     7681 cgggaaagcg caggccacca caaccgggcg gatcgggtat ctcggggtac ggaatctgga
     7741 tgaccaacgg atcgccggcc ggcacgtaag ccgaccgctc gaatggattg accggcaagg
     7801 tcaccgccag cagcaaccat gcatgcaagg ccaacgcgcc ggcgatggcc gacacccgca
     7861 ctgcattggg ccgctcgccg cgccgcgacg atgcccgcgc ctggaggctc tcgccttcgc
     7921 ctgccctgtc catgagcccc aaccttgtgc gacctgggtc acaagaatat gcgtaagcac
     7981 cgttaagatc gcaagcccga acacgaccgt ccgtcgaaca gtccccatgg ccccaacgcc
     8041 gaacccgccc cgcatcgtcg ccctgtccga actcgccgac gcctaccgcg tccccgacta
     8101 cctgcccgcc cactggcgca tcctcgtcgt ccccggcgac ctgcgcgtcg acggcgacct
     8161 gcaactggaa tgggaccgcc ccgacggcaa ggccaacccg ccgcggcacg actggcgcga
     8221 cgccgccggc tgcccgcccg acgaagccat cgacggcgtt gccatcgaag gcagcctgca
     8281 cgtcggcggc gcgatcatca gccactgggc cggcccggtc ctgctggtac gcgagcacct
     8341 gcgcgcgcgc agcctgatcg ccggcggcgc gttcatccgc atccgcggcg acgccgacgt
     8401 gaccgacacc gtgctcggcc ataccaacca cggcgagctc tacatcgacg gcaccgcgac
     8461 cgcgccgctg ctgatctggg acgaccactt cttcaacgcg gccaccatca acgccccgta
     8521 caccttcgac acccgcgcgg ccgcgtggga ggactgggag tacgacgacg acctggacgg
     8581 ttatccggtg cccgaacagt tgaagccggt gctgagggac gagtttgggt gctgggatga
     8641 agtgatggat gcgttgcggg cggggaagga cgtggtgagg cgcgacccgt aagtctctat
     8701 agcttgataa cgggtgaacc gctttgctgc tgaactggcg cctgcgattg gctctgcgcc
     8761 tgttcctggg cccgctcctt gctaagcgcg tccaactgct gcacagattc gcgcactggc
     8821 gcctgcatgg ccggatcagc gggtacgtgc gcccgcaggt gcgcggggtc ggacaatccg
     8881 ccctcgacgg caaacagatt gcgccggccg tccgatattg gactacccag cacgacgtga
     8941 tcggggtgcc gaatacccgc cagccgacat tgcaccgcca gattggccgc aatgcagtcc
     9001 agcgcctgcc cggcaggcat cgaattctgg cccgccaacg cgccgggcaa cttgtcatgc
     9061 agttggcgaa tcaaagcccg gtcggccgga tcggatggat caacggaagc atccgtttgc
     9121 gacggcgaag gtcgcagcag gcgttggtaa tcgcgaagat agagatcgcg ctgggtcagg
     9181 cgaccatcct gattttgatc ctccaggcca tcggcacgcg tgtacggttg atcgacgacg
     9241 accttggcca tcgccttgat caccgatgca tcgtccaggc ttccatcctt ccagccgtca
     9301 tataactgtg gccagtccaa gcccagcgtg ctgtagccaa gatcgctcca cctgggcctg
     9361 aacgacggct cgcttttact cagatcggca aagatgggaa atgcgttgaa gatcgcccga
     9421 gcttccttct cgctgcgaaa ttgaacgtag tcgtcggcac ttgcgttcac gggaaaaacc
     9481 gcatgacgat ccttgtcgtg acccacctcg tgggccatgc gcgaaaacaa tcccgccacg
     9541 agatgcgccc cctgaggggt cttcgcaaac tcgtactgac tcttgttgac gagaatttcg
     9601 cgatgcggtt ccgatcgata ggcggctaaa ggctcggaag gatctgccac caaccgcccg
     9661 ccattggaga agaattcctt gaaatcggca ctggcatggg aggacttcaa aaccagcgac
     9721 tgaaactcct ggggcagatc gaggaactca gcattgcgag ccagaaactg tgtattttcc
     9781 tcgtgtcgct tgcgttcttc atcggtcata ccccccccaa ctgatccatc aacgatcttg
     9841 gcttctatac tgcgtttgac ctgtcgagca ccgaaggacc ggaacatggc gaccaagctc
     9901 tacatcacct caatccgatt gacctttggc tggcttgcct tcgcgaccgt gattgccggc
     9961 tgcaccagcg caccatcgat cggcacgacg ccttctgcat caagcttgtc gatagaacag
    10021 ctggtgcgat tgcccgccga aaacgggcac gcgggcctcg atcaagtggt tcgcgccctg
    10081 caggcacgct atggcgaacg ctcgaacgtc gaactgtctc cgctcaccaa tactgcgacg
    10141 cccattaccc tggccgacgg cacggtcatc actcaggcag tcaccaacat caacggcagc
    10201 gacgacgtgg cgagggacgt attcctgggg attgcggaac gtccctgctt ggatgcccat
    10261 cgcattgcgg caatgatcgg cgcgaagaag ggctacgagg tggatggcga tgacatgacc
    10321 accggccatt cgagctattt cttccaaagc gcgcagatcg acatcgaaat ggaggcgcag
    10381 cgccaaggcc cggcttgcgt tcgttccatc gccatctaca agcgcctctc gcaataagct
    10441 ttgcctcgcc agtaaacaag cgcgagaaaa cctccgcatc accacggccg atgcctgccg
    10501 atcgcgctgc acaacgcatc gttgtggctc cccgcctccc gctcccgctc acagcgccga
    10561 acccggcatt acctcgtggg caatcggcat ctcgctcaca gcgccagtgc tcacctctct
    10621 catccatgac tcgatcctcc gcgatactta cctgcaatga cgcgtcttat cctagcgcca
    10681 gttatcgagg gatcgaccat gaaaagcaga ccagggcacc caatgcgcaa ctccatgttt
    10741 cgcatttccg cgttggctgt atttttggtg agctgcaaca cccataaaac caaatccgca
    10801 tccgatgcgc tcgacaccca aagcctgatt cgagtacccg cacaacgcgg cgccgcgggt
    10861 gtcgatcaag tgacttccgc actgcaagcg cgctacggcg ttgcctcagg cacgccactg
    10921 tccccgttcg tcaacagcaa ggctccggtt tcgctcgatg acggcgcggt cattgtccaa
    10981 accgtgacca atcctgacgc ccgcgatgtg cgagtagacc tcgcagccaa gccttgtctg
    11041 cccgtcgcac gcgcggccga gtggatcggc gccaagcgca tgctcgccac ggccggcagc
    11101 gacatcgtca cggggcatgt ggactatcgg ttcgatagcg ccgaggttcg catcgacctg
    11161 accggcgaat acggcggcga ggaatgcctt cgggtgattg aaatttacaa gcagtagcag
    11221 ccgtgcagta accgccgtaa tgacgtggcg acgttgacgc agcgaaggat cg
//
