LOCUS       NZ_CP076103            11272 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Lysobacter capsici strain NF87_2 chromosome, complete genome.
ACCESSION   NZ_CP076103
VERSION     NZ_CP076103.1
KEYWORDS    RefSeq.
SOURCE      Lysobacter capsici
  ORGANISM  Lysobacter capsici
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Lysobacter.
REFERENCE   1  (bases 1 to 6315468)
  AUTHORS   Liu,Y., Qiao,J., Liu,Y., Liang,X., Zhou,Y. and Liu,J.
  TITLE     Characterization of Lysobacter capsici strain NF87-2 and its
            biocontrol activities against phytopathogens. European Journal of
            Plant Pathology
  JOURNAL   Eur J Plant Pathol 155 (3), 859-869 (2019)
REFERENCE   2  (bases 1 to 6315468)
  AUTHORS   Liu,Y., Qiao,J. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) Institute of Plant Protection, Jiangsu
            Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing
            210014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: nextDenovo v. 2.0-beta.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018732085.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 13:41:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,303
            CDSs (total)                      :: 5,240
            Genes (coding)                    :: 5,174
            CDSs (with protein)               :: 5,174
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 16 of 66
            Pseudo Genes (incomplete)         :: 52 of 66
            Pseudo Genes (internal stop)      :: 2 of 66
            Pseudo Genes (multiple problems)  :: 4 of 66
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:02:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5077930
            Orig. end                         :: 5089202
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076103.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11272
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="10"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11272
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11272
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6272](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6272)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(719..732)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.86"
                     /tool="antismash"
     misc_feature    804..823
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.27"
                     /tool="antismash"
     misc_feature    complement(1357..1368)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.57"
                     /tool="antismash"
     gene            complement(1645..3195)
                     /locus_tag="KME82_RS21160"
                     /old_locus_tag="KME82_21160"
     CDS             complement(1645..3195)
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343478.1"
                     /locus_tag="KME82_RS21160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_21160"
                     /product="TROVE domain-containing protein"
                     /protein_id="WP_215495749.1"
                     /transl_table=11
                     /translation="MANLNLFASTRGPALPAATSCNEAGGLAYARRPESALALYAATGC
                     LNNTFYASAEQQLDHVLRLCAQVEPAFVAKTAIYTRQRGHMKDMPALLLASLTSRDGEA
                     FERAFGRVIDNGRMLRNFVQIVRSGQVGRKSLGSRPKRLVRQWLDRASVDQLLSAAIGN
                     QPSLADVIKMVHPKPADAERAALYAWLLGKPVDEAALPAKVRAYEAFKRSQSASMPKLP
                     MQYFSSLPLTDAQWIYLARTVSWQTLRMNLNTFQHHGVFNGSKVVKEIAERMRDRDEIR
                     NARVFPYQLLSAYHAAAGLPVEIVEALQDAMEIATRQVPALEGDVVVAVDVSGSMASPV
                     TGYRKGATTKMRCVDVAALVAACIQRNHKGARVMPFDTEVRKLRLNPRDSVMTQATQLA
                     GLCGGGTAVSAPLAQLNRDKSRVDLLVLVSDNESWRDTRNGGATATMREWAQIKARCPD
                     ARMVCIDLQPTATSQTVESSDVLHIGGFSDAVFDLLAVYAAQGGAATRWVERIDSITL"
     misc_feature    complement(3231..3244)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.31"
                     /tool="antismash"
     gene            3374..4966
                     /gene="rtcR"
                     /locus_tag="KME82_RS21165"
                     /old_locus_tag="KME82_21165"
     CDS             3374..4966
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008134 - transcription factor binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="rtcR"
                     /gene_functions="regulatory (smcogs) SMCOG1112: sigma-54
                     dependent trancsriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914660.1"
                     /locus_tag="KME82_RS21165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_21165"
                     /product="RNA repair transcriptional activator RtcR"
                     /protein_id="WP_215495750.1"
                     /transl_table=11
                     /translation="MKRQVVIGMLGTQLDAASGPGRWEKWRPTVSLGMHEDFLLDRLEL
                     IVDERRFGKLAHLVAQDLAQVSPETQVRRHDTFLADPWEFESVYGVLHDFVRAYDFQPE
                     DEDYYVHITTGTHVSQICWFLLTESRLFPGRLLQTAPPRKQTGEAPGTYAVIDLDLSRY
                     DRIAQRFAQERLHDRDLLKSGIATRNPAFNRMIEQIETVATRSKSPMLLMGPTGAGKSQ
                     LARRVFELKKQKHQLPGRFVELNCATLRGDGAMSTLFGHIKGAYTGASTDRAGLLRSAH
                     QGLLFLDEIGELGQDEQAMLLRALEEKRFLPVGGDKEVESDFQLIAGTNRDLQVAVIEG
                     RFRDDLLARLNLWTYRLPGLAERAEDIEPNLDFELERHSRENRQRVTFNREARERYLRF
                     ARSGEATWGGNFRDLGASVMRLATLAEGGRIRLDLVDEEIERLRRQWHGADAQRSLLDE
                     LLGEGAAQLDRFDRVQLEDVLRVCRQAKNLSQAGRELFAVSRSLKASSNDADRLRKYLA
                     RFGLDWEQLRAGA"
     gene            complement(5001..6272)
                     /locus_tag="KME82_RS21170"
                     /old_locus_tag="KME82_21170"
     CDS             complement(5001..6272)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="KME82_RS21170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_21170"
                     /product="YcaO-like family protein"
                     /protein_id="WP_215495751.1"
                     /sec_met_domain="YcaO (E-value: 2e-39, bitscore: 134.5,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIPYERERSLDEANRIIHACIARRGWTADTGEFGHGLVTIKCALH
                     DAQGRLLSRGSGKGEPAASLTGALYEAVEHYYCRASALATAPRFVSARELADDPRCAGL
                     PFVSEFADQSQRHLACRQYASYSGRPDLDVPLFMVFPDYPPAPELRDPRDDFDYHSATR
                     YSSNSGTAIGASFEEAALHGIGEIVERDAWALFMLAHYLGAPARYGRLIEPSSLPAELA
                     RIHDLAQRRLERRVLLIEVTSDLAYPAFIATTEERLPEEIVFPHGCGASPYAAHAATRA
                     LTELTQCVDIKENAPHLVELDQLALDLLLDYPKLRACVMGEIDPRRLHHAHWDYPQLPW
                     QAPKQLLPQVVADLEARGIGLYHAINHREGDEFCVVSSVSMELERFFLASTGLLMAPGR
                     RGRECLARQALAAQAQCEAQPQEA"
     gene            complement(6358..6537)
                     /locus_tag="KME82_RS21175"
                     /old_locus_tag="KME82_21175"
     CDS             complement(6358..6537)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KME82_RS21175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KME82_21175"
                     /product="hypothetical protein"
                     /protein_id="WP_215495752.1"
                     /transl_table=11
                     /translation="MGATRLDFEKTVLEKPKYIPATEALRVSSAASGVDFKVAMSELNA
                     PTAQEQAFLDEFAK"
     gene            complement(6885..7709)
                     /locus_tag="KME82_RS21180"
                     /old_locus_tag="KME82_21180"
     CDS             complement(6885..7709)
                     /GO_function="GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /locus_tag="KME82_RS21180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_21180"
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="WP_215495753.1"
                     /transl_table=11
                     /translation="MKASFVVVLSLLLLGFNASAQNRASRLPYPYIEPDAGADEPPKVQ
                     ACFLLYDLSAGEIRRTPSPACKRRVTPAATFDIPHALAGLDGSIVRAGQPLAGSGTHSL
                     DTALMYSSTEYFQFIAQRLGDTREREYMNRFNYGNADTSSGSTFWMGGSLQISPDEQML
                     FLRRFFSEQLPVNKQAMADVRAGLRQPPGAMIGNYGAVAIGRFPARVDTSVVAKSGSAV
                     MAGNEAVRWQVGQITRGTRSFVFVSCVTGPLNLPEGAAATFAANELRAANVL"
     gene            7959..9851
                     /locus_tag="KME82_RS21185"
                     /old_locus_tag="KME82_21185"
     CDS             7959..9851
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="resistance (resist) ABC_efflux"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974246.1"
                     /locus_tag="KME82_RS21185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_21185"
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="WP_215495754.1"
                     /transl_table=11
                     /translation="MSATPPVGPPAAEPPAVDPTAVAAHSSADPAATSAEVTPLARAAG
                     HRGDWSVLTALAPYLRPYLGRIAIALAMVFAAKLLNLYVPMALKQLIDRLNVPLTPLLL
                     PVGLLLSYGAARIGVTLFTELRQVVFARVMARTSRQVTLQVFQHLHGLSLKFHLARRTG
                     GVARDVERGGSAIADLLDWTIYTIIPVLLEVAMVTTVLVWIYDWRFAAIAIATLLLYGL
                     WTFSVTEWRTRYYRAQVEADTHANERAVDSLLNYETVKYFNNEDHEARRYDDNLRQLEN
                     AQVLSIKTLAVLNLGQSLVVALGVTAMMWLAARGVARGEMTVGDLVLVNAYLLQLSAPL
                     FMLGMMYREVKQALTNMERLFGLLDERQDVRDAPDAQTLASARPRVAFRNVRFGYDPRR
                     EILQGIDFDIPAGATVAVVGHSGSGKSTLARLLYRFYDVDSGAIEIEGHDLRALTQSSL
                     RGAIGIVPQDTVLFNDSIGYNIRYGRPDADDAQVEAAARAAHIHDFIVGLPDGYDTPVG
                     ERGLKLSGGEKQRVAIARALLKNPSILIFDEATSALDSKSERAIQSELDRLAVGRTTLV
                     IAHRLSTVMDADQILVMDAGRILERGTHSQLLAKNGIYAQMWELQQRQADEVVV"
     gene            complement(10289..10816)
                     /locus_tag="KME82_RS21190"
                     /old_locus_tag="KME82_21190"
     CDS             complement(10289..10816)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /locus_tag="KME82_RS21190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KME82_21190"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_215495755.1"
                     /transl_table=11
                     /translation="MNHSASHSDTPPIEIVGNVRIVDFQPRWRGDFARLNIEWLERWFV
                     VEPIDREVLNDPETHLLAGGGRVLFAVDDADRALGTVALLRYDDGLVELTKMAVEPGLR
                     GGGIGRKLMAGAIAAFQDMGGRELFLESSHRLGPALKLYESVGFRHHPAPRPGSHYQRA
                     DVYMIWESPAAP"
ORIGIN
        1 atgcttcatc atgatctcgc ggttgctgcg cgcgaactgc tgcgcccact cgactgcatg
       61 cacgtagtcg ttgtagtgat cgctgccttc gggcaagtaa gccagatcct ggtcgggcag
      121 attgatcatc cagcggcgca tgtcctgctt ggccaactcg atgaaatggc tgccgatcgc
      181 gttgccgacc ccgcgcgagc ccgagtgcag catgaaccac acgcggttct cctcgtccag
      241 gcaaacctcg acgaagtgat tgccggtgcc gagcgtaccc aggtgattca gatgattgct
      301 gcgctcgagc ttcgggtgct tggcgacgat gcgatcgaaa ccttcatgca gacccgacca
      361 tcgctgcaac gaagcctcgg gcggattcgc ccaggcgccc ttgtcgcgca gtccgcggcc
      421 gacggtgcgg ccgtgcggca ccgctttctc gatcgcggcg cgcacctcgc ccaaccggtc
      481 cggcaggtcc tctgccgtca acgtggtgcg caccgcgatc atgccgcagc cgatgtcgac
      541 accgaccgcc gccggcacga tcgcgcccac ggtcggcacc accgagccca cggtcgcgcc
      601 cttgcccaga tgcacgtcgg gcatcaccgc gatccagcgg tggatgaagg gcagcttggc
      661 gatgttctgc aactggcggc gcgcctcgtc ttccagcggc acgccgcggg tccagtgctt
      721 gatcggcacc gagcccgggt cgttgatcac gtcgtattga acggtggcgt gagtattggc
      781 gttcatggtc ttgctctctg tgttgcgggc cttgcggccc tcgctcccgc cgtcgctcct
      841 ggacgacggg aaggtgaagg atgcagcgtc gcggcgcatg cggccagcgc tggtcagcgc
      901 ccgggcatga cgaccgctcg cggcggtaaa gggcgcggca cgatgcgacc agtagaagcg
      961 gaacattgaa cttggaaggt tgtagttccg cccgcattcg catcgtgacg cgtttgaagc
     1021 aaaagccgat gcatcgcgac caggtttacg cggaacatcc tgtgctctac ccgactgagc
     1081 taccgtctcg caacgggcgg gactcgaacc cgcgacaaca gatcaatgta gttccgcaaa
     1141 gcattcgcga tacagcggta aaacggttgc agcgtgtcgc agaccgatga atagcctttt
     1201 cccgtaatct tccagatcgt cattcccgcg aacgcgggaa tccagtgtct ttaacgcgac
     1261 ctgcacgaaa gtcgctggat ccccgcgttc gcgggaatga cgaactggca gattcgtaaa
     1321 gcctagaaaa aatcgacata tcacgactag aaacgacgaa acgttttcct gctctatcca
     1381 ctgagctacc gtcctgcgac gggtgggatt cgaacccacg acctggcacg tgataggtgt
     1441 agtttcgtca agcattcgcg atacatcgac aaaccaaaaa cctgggccgg ccggcggacc
     1501 ctattgtccg ccggcccttc ctgcaccgca tcgcacctcc atggcgcgtg cggccgacgg
     1561 accgtcctgg tccgtccgcg cgaacgtatc cctcgttcgc gttgcggcgc ctccctgcgc
     1621 cgcgcggcac cgccctgttg ccgcttacag cgtgatcgaa tcgatccgct ccacccatcg
     1681 cgtcgccgcc ccgccctgcg ccgcgtacac cgccaacagg tcgaacaccg cgtcgctgaa
     1741 gccgccgata tgcaacacgt ccgacgactc gaccgtctgg ctggtcgcgg tcggctgcaa
     1801 gtcgatgcag accatccgcg catccgggca acgcgccttg atctgcgccc actcgcgcat
     1861 cgtcgcggtc gcgccgccgt tgcgggtgtc gcgccagctt tcgttgtcgg acaccagcac
     1921 caacagatcg acccgggact tgtcgcgatt caactgcgcc aacggcgcgc tgaccgcggt
     1981 gccgccgcca cacaagcccg ccaactgcgt cgcctgggtc atcacgctgt cgcgcgggtt
     2041 caagcgcagc ttgcgcactt cggtgtcgaa cggcatcacc cgcgcgccct tgtggttgcg
     2101 ctggatgcag gccgcgacca gcgccgccac gtcgacgcaa cgcatcttgg tggtcgcccc
     2161 cttgcggtaa ccggtgaccg gcgaagccat cgaacccgac acgtccaccg cgaccacgac
     2221 atcgccttcc agcgccggca cctggcgggt cgcgatctcc atcgcgtcct gcaaggcctc
     2281 cacgatttcc accggcaagc cggcggcggc gtggtacgcg ctcaacaact ggtacggaaa
     2341 cacgcgggcg ttgcggattt catcgcgatc gcgcatgcgc tcggcgatct ccttgaccac
     2401 cttcgagcca ttgaacacgc catgatgctg gaacgtgttg aggttcatcc gcaacgtctg
     2461 ccacgacacc gtccgcgcca ggtagatcca ttgcgcatcg gtcaacggca acgagctgaa
     2521 gtactgcatc ggcagcttcg gcatcgacgc ggactgcgag cgcttgaacg cctcatacgc
     2581 ccgcaccttg gccggcagcg ccgcctcgtc gaccggcttg ccgagcaacc acgcatacaa
     2641 cgcggcgcgc tcggcatcgg ccggcttggg gtggaccatc ttgatcacgt cggcgagcga
     2701 gggctgattg ccgatcgccg cgctcaacag ctggtccacc gacgcgcggt cgagccactg
     2761 ccgcaccagg cgcttgggac gcgagcccag cgacttgcga cccacctgcc cgctgcgcac
     2821 gatctggacg aagttgcgca gcatgcggcc gttgtcgatc acccggccga acgcgcgctc
     2881 gaacgcttcg ccgtcgcgac tggtcaggct ggccaacaac agcgccggca tgtccttcat
     2941 atggccgcgc tggcgggtgt agatcgcggt cttggcgacg aacgccggct cgacctgcgc
     3001 gcacaacctc aacacgtgat cgagctgctg ctcggcgctg gcgtagaagg tgttgttcaa
     3061 gcagccggtg gcggcgtaca gcgccagcgc cgactccggg cggcgcgcat aggccaggcc
     3121 gccggcctcg ttgcacgagg tggccgccgg cagcgccggg ccgcgggtcg aggcgaagag
     3181 gttgaggttg gccatcgggg cattccgggt tgggacttgc ctgtactaga gcaagcgccg
     3241 tgccaagttc ccgggacagc cattaaccat ctgaattaat aggaatttat agttttaaca
     3301 ggccaccaac cccgaaaccc ggtaaagatt tatctatact ttcagatata aatttatcca
     3361 tgaagatatc tccatgaagc gccaggtcgt catcggcatg ctcggcaccc aactcgacgc
     3421 ggcctccggc ccgggccgct gggaaaaatg gcgccctacc gtgtcgctgg gcatgcacga
     3481 ggacttcctg ctcgaccggc tggagctgat cgtcgacgag cgccgcttcg gcaagctcgc
     3541 ccacctggtc gcgcaggatc tggcccaggt ttcgccggaa acccaggtgc gacgccacga
     3601 caccttcctc gccgatccct gggagttcga atccgtctac ggcgtgctgc acgacttcgt
     3661 gcgcgcctac gatttccagc ccgaggacga ggactactac gtccacatca ccaccggcac
     3721 ccacgtcagc cagatctgct ggttcctgct gaccgaaagc cgattgttcc cgggccgcct
     3781 gctgcagacc gcgccgccgc gcaaacagac cggcgaggcg ccgggcacgt atgcggtgat
     3841 cgatctggac ctgtcgcgct acgaccgcat cgcgcagcgc tttgcgcagg agcggctgca
     3901 cgatcgcgac ctgctcaaga gcggcatcgc cacgcgcaat cccgccttca accgcatgat
     3961 cgaacagatc gagaccgtgg ccacgcgttc gaagtcgccg atgctgttga tgggcccgac
     4021 cggcgccggc aagagccagc tcgcgcggcg cgtgttcgaa ttgaagaagc agaagcacca
     4081 actgcccggc cgcttcgtcg aattgaactg cgcgaccctg cgcggcgacg gcgcgatgag
     4141 caccttgttc ggccatatca agggcgccta caccggcgcc tcgaccgatc gcgccggcct
     4201 gctgcgttcg gcgcaccagg gcctgttgtt tctcgacgag atcggcgaac tcggccagga
     4261 cgaacaggcg atgctgctgc gcgcgctgga agaaaagcgg ttcctgccgg tcggcggcga
     4321 caaggaagtc gaaagcgatt tccagctcat cgccggcacc aaccgcgatc tgcaggtcgc
     4381 ggtgattgag ggccgttttc gcgacgacct gctcgcgcgc ctgaatcttt ggacctatcg
     4441 cctgcccggc ctggccgagc gcgccgagga catcgagccg aacctggatt tcgaactcga
     4501 gcgtcactcg cgcgaaaacc gccagcgcgt gaccttcaat cgcgaagcgc gcgaacgtta
     4561 tctgaggttc gcgcgcagcg gcgaagcgac ctggggcggc aacttccgcg acctgggcgc
     4621 gtcggtgatg cggttggcga ccctggccga gggcggccgc atccgtctgg atctcgtcga
     4681 cgaggaaatc gaacgcctgc gccggcagtg gcacggcgcg gacgcgcagc gctcgctgct
     4741 cgacgagctg ctcggcgaag gcgcggcgca actcgatcgc ttcgaccggg tgcagctgga
     4801 agacgtgctg cgcgtatgcc ggcaggcgaa gaacctctcg caggccgggc gcgagctgtt
     4861 cgcggtgtcg cgctcgctca aggccagcag caacgacgcc gaccggttgc gcaagtacct
     4921 ggcgcggttc gggctcgact gggagcaatt gcgcgccggg gcttgacccg ggcgcgtcac
     4981 acggcgacca ggccaggcga tcaagcctcc tgcggctgtg cctcgcactg cgcctgcgcc
     5041 gccaacgcct gacgcgccag acactcgcgc ccgcgccgcc ccggcgccat caacaaaccg
     5101 gtgctggcga ggaagaagcg ctccagttcc atcgacacgc tgctgaccac gcagaactcg
     5161 tcgccttcgc ggtgattgat cgcgtggtac aggccgatgc cgcgcgcttc cagatcggcc
     5221 acgacctgcg gcagcaactg cttcggggcc tgccacggca actgcggata gtcccagtgc
     5281 gcatgatgca ggcgtcgcgg atcgatctcg cccatcacgc aggcgcgcag cttggggtaa
     5341 tccagcagca ggtccaacgc gagttgatcg agttcgacca gatgcggcgc gttttccttg
     5401 atgtcgacgc attgggtcag ctcggtcaat gcgcgcgtcg ccgcatgcgc ggcgtagggc
     5461 gaggcgccgc agccgtgcgg aaacacgatt tcctcgggca agcgctcttc ggtcgtggcg
     5521 atgaaggccg gataagccag gtcgctggtc acctcgatca gcaacacgcg gcgttcgagc
     5581 cggcgctgcg ccaagtcgtg gatgcgcgca agctcggccg gcagcgacga cggctcgatc
     5641 aagcggccgt agcgcgccgg cgcgccgagg taatgcgcga gcatgaacaa tgcccaggca
     5701 tcgcgctcga cgatctcgcc gatgccgtgc agcgcggcct cctcgaagct ggcgccgatc
     5761 gcggtgccgc tgttggagct gtagcgggtc gcgctgtggt agtcgaaatc gtcgcgcgga
     5821 tcgcgcaact ccggcgcggg cggatagtcg ggaaacacca tgaacaacgg cacgtccaga
     5881 tcggggcgac ccgaatacga cgcgtactga cggcacgcca gatgccgctg ggattgatcg
     5941 gcgaactcgg acacgaacgg caagccggcg cagcgcgggt cgtcggcgag ttcgcgcgcg
     6001 gatacgaacc gcggcgcggt ggccagcgcg gacgcgcggc agtagtagtg ttcgaccgct
     6061 tcgtacaagg cgccggtcaa cgaggcggcc ggctcgccct tgcccgaacc gcgactgagc
     6121 agtcgcccct gcgcatcgtg cagggcgcac ttgatcgtga ccaggccatg gccgaactca
     6181 ccggtatccg cggtccagcc gcgccgcgcg atgcaggcgt ggatgatccg gttggcctcg
     6241 tccaggctgc gctctcgttc gtacggaatc attccttgtt cctcgccaat ggtgcatgcg
     6301 gtgcatgaga gcgaacgcat gacgcgcgac agtgcgcgcg ccatgcgggt tcagcgctta
     6361 cttggcgaac tcgtccagga acgcctgctc ctgcgcggtc ggcgcattga gctcgctcat
     6421 cgcaaccttg aaatccacgc cggacgcggc gctgctgacg cgcagcgctt cggtggcggg
     6481 gatgtacttc ggcttttcga gcaccgtctt ttcgaaatcg aggcgggtag cacccatgac
     6541 taaacctcct gttaggtcga aagaggcacg gacgcgaacc gacgaggcgt tgtccaccag
     6601 gctgcgggcc gtccctgtgc cgcagcctgc ggctgcgcga tcagggttac ccgttgcgac
     6661 ggacacaggt ccatgaaacg cgtcaccaaa ccgcgcagcg acgacgcaac cgggcgacgc
     6721 gcggcgtcga tcgcggcaaa tacggttcaa acctgaaaca tcgcgggcgc cttatgtgcg
     6781 ccaacggcaa tccacgcatg ggcgcacacc gcgacggatg tctcagtcgc ggcgcccggt
     6841 cgatcgagtg aacacggcag cggcgaatgc ggctgccgcg cagatcacag cacgttggcc
     6901 gcgcgcaatt cgttcgcggc gaaggtcgcc gcggcgccct cgggcaggtt caacggaccg
     6961 gtcacgcagc tgacgaacac gaacgagcgc gtgccgcggg tgatctggcc gacctgccag
     7021 cgcaccgctt cgttgccggc catcaccgcc gagccgctct tggcgaccac cgaagtgtcg
     7081 acgcgcgcgg gaaaacgccc gatcgcgacc gcaccgtaat tgccgatcat cgccccgggc
     7141 ggttgccgca ggccggcgcg cacatcggcc atggcctgct tgttgaccgg caactgctcg
     7201 ctgaagaagc ggcgcaggaa cagcatctgt tcgtccggcg aaatctgcag cgagccgccc
     7261 atccagaagg tcgaaccgct gctggtgtcg gcgttgccgt agttgaagcg gttcatgtac
     7321 tcgcgttcgc gggtgtcacc caggcgctgg gcgatgaact ggaagtactc ggtcgacgaa
     7381 tacatcagcg cggtgtcgag cgaatgggtg ccgctgcccg cgagcggctg gccggcgcgc
     7441 acgatgctgc cgtcgagccc ggccagggcg tgcggaatgt cgaaggtcgc cgccggcgtg
     7501 acccggcgct tgcaggccgg cgacggggtg cggcggattt cgccggcgct caggtcgtag
     7561 agcaggaaac aagcctggac cttgggcggc tcgtcggcgc ccgcgtcggg ctcgatgtac
     7621 ggatacggca gccggctggc ccggttctgg gcgctggcgt tgaagcccag caacagcagg
     7681 ctcaaaacga cgacgaatga ggctttcatg cctggacgac tccttagcag gaagggcgcg
     7741 agcggccgca ggggccgtgg cgcacctgcg ccggcctgcg tccgtgccgc gcggaccgga
     7801 ccagaatgcc atgcccggca ggcacattcg accgcggtcg gccgcagttt ccgtacccgc
     7861 cgacatacca cccgacttga gcggccttga cctgaagcgg gcttgaggtc ctatgctcgc
     7921 ctgcccgata ccgcgcgaac cgcttcgcga aggaccgcat gtccgccacg ccaccggtgg
     7981 gtccgcccgc cgccgaaccg cccgcggtcg atccgaccgc cgtcgctgcg cattcctccg
     8041 ccgaccccgc cgccaccagc gccgaggtca ccccgctcgc gcgcgccgcc ggccaccgcg
     8101 gcgactggtc ggtgctgacc gcgctcgcgc cgtacctgcg cccctacctg ggccggatcg
     8161 cgatcgccct ggcgatggta ttcgcggcca agctgctcaa cctgtacgtg ccgatggcgc
     8221 tcaagcagct catcgaccgc ctcaacgtac cgctgacccc gctgctgctg ccggtcggcc
     8281 tgctgctgtc ctacggcgcc gcgcgcatcg gcgtgacctt gttcaccgaa ctgcgccagg
     8341 tggtgttcgc gcgggtgatg gcgcgcactt cgcgccaagt aaccttgcag gtgttccagc
     8401 atctgcacgg actgagcctg aagttccacc tcgcccgccg caccggcggc gtcgcccgcg
     8461 acgtcgagcg cggcggcagc gcgatcgccg acctgctgga ctggacgatc tacacgatca
     8521 tcccggtgct gctcgaagtg gcgatggtca ccaccgtgct ggtgtggatc tacgactggc
     8581 gcttcgccgc gatcgcgatc gcgaccttgc tgctgtacgg gctgtggacg ttctcggtga
     8641 ccgaatggcg cacgcgctac taccgcgctc aggtcgaggc cgacacccac gccaacgaac
     8701 gcgcggtcga ctcgctgctc aattacgaaa cggtcaaata cttcaacaac gaagaccacg
     8761 aggcccggcg ctacgacgac aacctgcgcc agctggagaa cgcccaggtc ctctcgatca
     8821 agaccctggc cgtgctcaac ctcggccaga gcctggtggt cgcgctcggc gtgaccgcga
     8881 tgatgtggct ggccgcgcgc ggcgtggcgc gcggcgagat gacggtcggc gacctggtgc
     8941 tggtcaatgc ctatctgctg cagttgtcgg cgccgttgtt catgctcggc atgatgtacc
     9001 gcgaagtgaa gcaggcgctg accaacatgg agcggctgtt cggcctgctc gacgaacgcc
     9061 aggacgtgcg cgacgcgccc gatgcgcaga ccttggcctc ggcgcggccg cgggtggcgt
     9121 tccgcaacgt gcgcttcggt tacgacccgc gtcgcgagat tctgcaaggc atcgatttcg
     9181 acatcccggc cggcgccacc gtggccgtgg tcggccattc gggctcgggc aaatcgaccc
     9241 tggcgcggtt gctgtaccgc ttctacgacg tcgactccgg cgcgatcgag atcgaaggcc
     9301 acgacctgcg tgcattgacc cagtcctcgc tgcgcggcgc gatcggcatc gtcccgcagg
     9361 acaccgttct gttcaacgac agcatcggct acaacatccg ctacggccgg cccgacgccg
     9421 acgacgcgca ggtcgaggcc gccgcgcgcg ccgcgcacat ccacgatttc atcgtcggcc
     9481 tgcccgacgg ttacgacacg ccggtgggcg aacgcgggct caagctgtcg ggcggcgaaa
     9541 aacagcgcgt ggcgatcgcg cgcgccctgc tcaagaaccc gtcgatcctg atcttcgacg
     9601 aagccacctc ggccctggac tccaagtccg agcgcgcgat ccagtccgaa ctcgaccggc
     9661 tcgcggtcgg ccgcaccacg ttggtgatcg cgcaccggct gtcgacggtg atggatgccg
     9721 accagatatt ggtcatggac gccggccgca tcctcgaacg cggcacccat tcgcagttgc
     9781 tggcgaagaa cggcatctac gcgcagatgt gggagttgca gcagcgccag gccgacgagg
     9841 tcgtcgtgta gccacaccgg ccctgcagga gcggcgcgag ccgcgaccgc gacctctcga
     9901 taacggcgca agcgatgttt cgcggtcgcg gcttgcgccg ctcctacagt cgggcaccgc
     9961 tcaacccgat cgcctgcgac aacgccaaac catccaatcg gcgcataacc tcatgcaacc
    10021 ccctgtagga gcggcgcaag cagcgaccgc gacctctcgg taacggcgca agcgatgttt
    10081 cgcggtcgcg gcttgcgccg ctcctacagt cggacaccac ccaacccgat cgcgtgcgac
    10141 aacgccaaac gatcaaatcg gcgtatgacc tcatgcaatc ccctgcagga gcggcgtgag
    10201 ccgcgatcgc gacctctcgg ccacggcgca agcgatgttt cgcggtcgcg gcttgcgccg
    10261 ctcctacagt cgaaggctcg ctagcgaatc agggcgccgc cggcgactcc cagatcatgt
    10321 acacatccgc ccgctggtaa tgcgagcccg gacgcggcgc ggggtgatgg cgaaacccga
    10381 cgctttcgta cagcttcaac gccgggccca ggcgatggct cgattcgagg aacaattcgc
    10441 gtccgcccat gtcctggaac gcggcgattg ccccggccat caacttgcgg ccgatgccgc
    10501 cgccgcgcag gccgggctcg accgccatct tggtcagctc gaccagcccg tcgtcgtaac
    10561 gcagcagcgc caccgtgccg agcgcgcgat cggcatcgtc gaccgcgaac agcacgcgcc
    10621 cgccgccggc gagcagatgg gtttccggat cgttgagcac ttcgcgatcg atcggctcga
    10681 ccacgaacca gcgctcaagc cattcaatgt tgagacgggc gaaatcgccg cgccagcgcg
    10741 gctggaaatc gacgatgcgg acattgccga cgatttcgat cggcggcgta tcggagtggg
    10801 aggcggagtg gttcatacct cgatgatagc cgcgtcggct gcggctggac gcagtaccaa
    10861 agtcgtatcc aaaaactgcg gccgccacct gggcgggagg cgccgtttcg gcggacgaag
    10921 aagcatcgcc gatccgtctg agcagattcc aaaccagcgc aatgcacgtc ggcaagaagc
    10981 cggcgccgga acgaaaacgg cgacctcgcg gccgccgtat ttgtcgcgaa cgctacccgc
    11041 acgactcagt cgcgcttggc ctcgcgggtg gccggcttgc cctggcgctt ggcggcgccc
    11101 tcgctcgcgg catggctttt agcggccgca cccttggcct tgcccgtctc ggctttgcct
    11161 ttgtcggcct tgcccttctc ggctttggcc acgaccggcg ccttcggcgg cgggtaatcg
    11221 cggtacggcc gattggggaa atcgcggtgc agttcggtat cgatcgtgat cg
//
