LOCUS       NZ_JAHHZH010000005     10888 bp    DNA     linear   CON 04-JUL-2024
DEFINITION  Pseudomonas sp. MG-9 NODE_5_length_602414_cov_64.0953, whole genome
            shotgun sequence.
ACCESSION   NZ_JAHHZH010000005
VERSION     NZ_JAHHZH010000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Pseudomonas sp. MG-9
  ORGANISM  Pseudomonas sp. MG-9
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 602414)
  AUTHORS   Lutfullin,M.T., Pudova,D.S., Lutfullina,G.F., Vologin,S.G.,
            Gogoleva,N.E., Shagimardanova,E.I., Sharipova,M.R. and
            Mardanova,A.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) Microbiology, Kazan Federal University, 18
            Kremlyovskaya, Kazan, Tatarstan Republic 420008, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2021
            Assembly Method                   :: SPAdes v. v. 3.12
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018729515.1-RS_2024_07_04
            Annotation Date                   :: 07/04/2024 03:27:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 89
            rRNAs                             :: 8, 9, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 6, 8 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 25 of 68
            Pseudo Genes (incomplete)         :: 48 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:31:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 154683
            Orig. end                         :: 165571
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHHZH010000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10888
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10888
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10888
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5888](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5888)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(739..748)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
     misc_feature    1124..1138
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 19.86"
                     /tool="antismash"
     gene            complement(1317..1970)
                     /locus_tag="KKQ10_RS14550"
                     /old_locus_tag="KKQ10_14550"
     CDS             complement(1317..1970)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007959149.1"
                     /locus_tag="KKQ10_RS14550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_14550"
                     /product="hypothetical protein"
                     /protein_id="WP_215501491.1"
                     /transl_table=11
                     /translation="MRVARSLIVVALLPLFAACQLLDGPRESAAHVGQTRMQGQLTAAD
                     GELVFQPCGEQRQYVVNDIGGTSVLQEAATLADQQGKLFADVRGKIAGDRLDLGQLYRV
                     ERSGTACDDPNFKLLILRAAGHTPEWNVKVSGKGMVIDREGQPALAVPYVEEQLGDGRF
                     NLSSEANNQRIELWVAPQRCVDSSTGSVQHMSAELRIDGKVQRGCGYFGGSRND"
     gene            complement(2039..2638)
                     /locus_tag="KKQ10_RS14555"
                     /old_locus_tag="KKQ10_14555"
     CDS             complement(2039..2638)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336817.1"
                     /locus_tag="KKQ10_RS14555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_14555"
                     /product="short chain dehydrogenase"
                     /protein_id="WP_127649649.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 6.9e-07, bitscore:
                     23.7, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKILLIGAAGTIGSAVDKELSQRHEVIRIGRSSGDLRVDISDSAS
                     IRQLFEQTGKFDALVCAAGNVTFAPLGEMNADSFALGLKDKLMGQVNLLLIGREYANDG
                     ASFTFTTGVLSHDPIRSGASAALVNGALDSFVKAAAIELPRGLRVNSISPTVLQEAMGS
                     YAPYFRGYKPVPAADVALAYAKSVEGLQTGQTFHVG"
     misc_feature    complement(2717..2730)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.35"
                     /tool="antismash"
     misc_feature    2721..2736
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 23.6"
                     /tool="antismash"
     misc_feature    complement(2722..2736)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 20.2"
                     /tool="antismash"
     gene            2742..3656
                     /locus_tag="KKQ10_RS14560"
                     /old_locus_tag="KKQ10_14560"
     CDS             2742..3656
                     /GO_function="GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007969471.1"
                     /locus_tag="KKQ10_RS14560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_14560"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_215501492.1"
                     /transl_table=11
                     /translation="MSEMDDLAAFAVLIEAGSFTLAAQQLGCSKGQLSKRISQLEAQFC
                     VVLLQRTTRRLSLTAAGAALLPQAQALVVQVERARQALARLKDDMAGPVRMTVPVSLGE
                     TFFDGLLLEFSQQYPEVQIELELNNHYRDLSRDGFDLAIRTEVANDERLVAKPLLAWQE
                     MTCASPAYLAQFGEPLTPQELVEHRCLLNSHYSGREEWLYHQQHELLRVRVSGPFASNH
                     YNLLKKAALAGAGIARLPSYLLQTELADGRLCWLLRDYQTRRMPMYLVHPYQGGLPKRT
                     QVLADYLIGWFKRSGEALDRLQR"
     gene            complement(3773..4249)
                     /locus_tag="KKQ10_RS14565"
                     /old_locus_tag="KKQ10_14565"
     CDS             complement(3773..4249)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018926902.1"
                     /locus_tag="KKQ10_RS14565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_14565"
                     /product="DoxX family protein"
                     /protein_id="WP_215501493.1"
                     /transl_table=11
                     /translation="MNNLIAHAIALLEKIPHSLIAFIARFSIAAVFWKSGQTKVEGLAI
                     DLIDGTAQLGWPHLADSTIPLFKSEYHVPLLSPEIAAHLAAFAEHFFPVLILIGFATRF
                     SALALLGMTLTIQLFVYPDAYPTHGTWAAVLLYLMATGPGKLSIDHLIARHYNR"
     gene            complement(4246..5004)
                     /locus_tag="KKQ10_RS14570"
                     /old_locus_tag="KKQ10_14570"
     CDS             complement(4246..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906665.1"
                     /locus_tag="KKQ10_RS14570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_14570"
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_215501494.1"
                     /transl_table=11
                     /translation="MSTQAAFAAALLDPQRPCPEGLCCANGADPASRFSVYRNNVQGSL
                     INALADSYPVVQQLVGEEFFRAMAAVFVQQQPPHSPLMSRYGEALPAFIDAFEPAASVP
                     YLADVARLERLRTCAYHAADALPVHPQQITAALAEPDGLSELRIKLHPSLHLLDSNHAV
                     VAIWAAHQGDATLAGIDIHHGQHALVLRNGLDVEVLALEPGASAFLRHLLDGQPLLAAA
                     QNSPPFDLSHTLALLIAHHAIVDLNDKESP"
     gene            complement(5001..5888)
                     /locus_tag="KKQ10_RS14575"
                     /old_locus_tag="KKQ10_14575"
     CDS             complement(5001..5888)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918539.1"
                     /locus_tag="KKQ10_RS14575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_14575"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_215501495.1"
                     /sec_met_domain="DUF692 (E-value: 9.2e-86, bitscore: 282.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFTLDEPVPTRTQAALPGLPRRAGLGLKTGHFREVLGSLPDLGFF
                     EVHAENYMVDGGPFHHFLGLIREQYPLSLHGVGLSIGAEGPLDVQHLQRLKQLIERYQP
                     QSFSEHLAWSSHGPVFLNDLLPLAYDMPTLNRVCAHIDQVQNTLKRPMLLENPATYLAF
                     QRSTIDEPDFIAEVVRRSGCGLLLDVNNVYVSCINHQRDPLAYLDALPLQAVGEIHLAG
                     FAEDNDSLGERLLIDDHGAPIDQAVWALYRQALHRTGAVATLIERDNQLPAFNVLLAEA
                     RQADEILLDAGVRS"
     misc_feature    complement(5882..5896)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.72"
                     /tool="antismash"
     gene            complement(5914..6219)
                     /locus_tag="KKQ10_RS14580"
                     /old_locus_tag="KKQ10_14580"
     CDS             complement(5914..6219)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008005836.1"
                     /locus_tag="KKQ10_RS14580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_14580"
                     /product="DUF2282 domain-containing protein"
                     /protein_id="WP_215501496.1"
                     /transl_table=11
                     /translation="MTATTRTLSATALVLALGSALSMAAISTVHAADADMEKCFGVAMK
                     GHNDCAAGAGTTCAGTSKMDYQANAWKLVPKGTCATTESKTSPTGFGQMQAFKAKS"
     misc_feature    6241..6253
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 20.68"
                     /tool="antismash"
     misc_feature    6318..6332
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 20.65"
                     /tool="antismash"
     misc_feature    6329..6343
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.96"
                     /tool="antismash"
     misc_feature    6470..6483
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.92"
                     /tool="antismash"
     gene            6523..8226
                     /locus_tag="KKQ10_RS14585"
                     /old_locus_tag="KKQ10_14585"
     CDS             6523..8226
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007969477.1"
                     /locus_tag="KKQ10_RS14585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_14585"
                     /product="DUF2599 domain-containing protein"
                     /protein_id="WP_215501497.1"
                     /transl_table=11
                     /translation="MTKDRSDSAALSRKVSSFALACTLPLLLSTVHAQPHPDPLDTLTR
                     INHNYNTLKDNCQEPDTGAARGLYYCSGVTLRMVNDGPFNPWDYSPYAIKIGATSYSWI
                     RKDLSTRILIHPAGFILRTPTDAAALKLPVKEQGFTCIYAFDGGTGPERKWYGCGFFDS
                     REPPRAAQGTMNNRNAALAYGTCAEAGVTTAEQWTQKYTGLMKGPIQYGQCSWNAEKPG
                     DWQAMIRVHESRPNTTNKDPFAFSAQVNEFMLKNATATNDGSENMKYIDAFIYNVNSTQ
                     NFATRGDQAPPKPENGLNSARNFQKKLKAQGYSVPILRLDFTQPAERRFSYVAADQAID
                     EMVVAGAPAPKYIADAVWIERYDPGTRKNEWSLKVTPTAQGKAAQATDQEAVYKELLAL
                     RGTDSQWRDNEKAAGSMRQQLSCLVQNYPVKTEWNLEPFRPVVSPQDAAKAGCNPVPVQ
                     APQYIASADWIKRYDPGTRQDEWSLSIVPTATGRALPNDQAGALYDQLFALKGADSNWR
                     DNEKSAGSMRQQLSCVLVNYRNKTPWNLEPFRPALSDSETRAAGCNPVPR"
     gene            8261..9499
                     /locus_tag="KKQ10_RS14590"
                     /old_locus_tag="KKQ10_14590"
     CDS             8261..9499
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014337985.1"
                     /locus_tag="KKQ10_RS14590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_14590"
                     /product="DUF2599 domain-containing protein"
                     /protein_id="WP_127649655.1"
                     /transl_table=11
                     /translation="MKKSLIRTVPLLLVPLLLHPAWANAESCAETLKKVETLYNNTVDS
                     CGQDPASDCSGLLVRGTHRADPAKGQKWDVWNPSPKAVELGTFAASYMRADGISYEDPG
                     MSTQNGYLITPRDLVRDPETPVHVYCAFPNDAWTDFRNDRGCGDNKNTAPTEAVCQAMK
                     PPVSTPNAWVAHFTQYNNNRQQDQLQCGFNMRNPMSSKERVDAFRNFMGARKVINSREF
                     QTQTELRLGNPKTDELPVLAFFYSDQRGLNDALANQKDYKAKTGKDRNIIKIDFPRTPV
                     AKASFSCIQTSTPTEPKFCDKYIESSTWVQRPDPKLGPNTWSLTVVPTACGRAIKDDQT
                     DRMFAELYNKHKDDQQWREYSVNGGSLRRQMVCHLAAVYDGKPVRNKPEWNLEPARPYV
                     DQATAVAQHCNPY"
     gene            complement(9665..10366)
                     /locus_tag="KKQ10_RS14595"
                     /old_locus_tag="KKQ10_14595"
     CDS             complement(9665..10366)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249761.1"
                     /locus_tag="KKQ10_RS14595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_14595"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_064586349.1"
                     /transl_table=11
                     /translation="MLQFENVSTFYGKIQALHSVNVEVRQGEIVTLIGANGAGKSTLLM
                     TLCGSPQAHSGSIRYMGEELVGQDSSQIMRKSIAVVPEGRRVFARLTVEENLSMGGFFT
                     DKGDYQAQMDKVLGLFPRLKERFNQRGGTMSGGEQQMLAIGRALMSKPKLLLLDEPSLG
                     LAPIIIQQIFDIIEQLRKDGVTVFLVEQNANQALKIADRAYVLENGRVVMQGTGEALLT
                     DPKVREAYLGG"
     misc_feature    complement(10343..10354)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.77"
                     /tool="antismash"
ORIGIN
        1 tgtaatcgct gccgccgagg atgaacgcgt gggattccag acgctcctgc agctcgatgc
       61 cagccagcgg gccgcccggg taatcgactt ccggggtgat gccgacgacg atcccggagt
      121 tggcgttacg ctcgttacgc gagtactggc tcatgccgtt ggtaaccacg cggttcggct
      181 cggacgtcgc cgccaccacg gtgccgcccg ggcacatgca gaagctgtag accgagcggc
      241 cgttcttggc gtggtgcacc agtttgtagt cggcggctcc caacttgggg tggccggcgt
      301 atttgcccag gcgcgcacgg tcgatcagcg attgcgggtg ttcgatgcgg aaacccaccg
      361 agaacggttt ggcttccatg aacacgccac ggctgtggag catgcggaag gtgtcacggg
      421 cactgtggcc gagggccagg accacgtgtt tcgaatgcag ggtttcgccg ctcgccagct
      481 cgacgccgac cagttggccg tcgtcgatca gcacgtcggt gacgcgctcc tgaaaacgta
      541 cttcgccgcc cagggcacgg atctcttcac gcatggtctc gaccatgccg gtcagacgga
      601 acgtaccgat gtgcggtttg ctgacgtaga ggatttcttc cggcgcgccg gccttgacga
      661 attcgtgcag gactttacgc ccgaggaact tcggatcctt gatctggctg tacagcttgc
      721 cgtcggagaa cgtgcccgcg ccgccttcac cgaactgcac gttggactcg gggttgagca
      781 cgcttttacg ccacaggccc caagtgtcct tggtgcgctg acggacttcg gtgccgcgct
      841 cgaggatgat cggtttgaaa cccatctgtg ccagcaacag gccggcgaag atcccgcatg
      901 ggccgaaacc gaccacgatc gggcgctggc tcaggtcgct tggcgcctga ccgacgaatt
      961 tgtagctgac atccggtgcc acgttgacgt tacggtcgtc ggcgaacttg cccagcacct
     1021 tggcctcgtc gcgcacgttg aggtcgatgg tgtagatgaa gcacagttcg gaggactttt
     1081 tgcgcgcgtc gtagctgcgc ttgaacaagg tgaaatcgag caaatcatcg ctggcgatgc
     1141 ccaggcgctg cacgatagcg gcacgcaggt cttcttcggg atggtcgatt ggcagcttga
     1201 gttcggtgat tcgtaacatg gcgaggatcc gggttcgcgg ggcgcacaac tgcgccaggg
     1261 cgtttgaagg ccgcgattat aagccgcaaa ggccggatcc ggtgaggcta aaacgctcag
     1321 tcgttgcgcg atccgccgaa atacccgcaa ccgcgctgca ccttgccgtc gatgcgcagt
     1381 tcggcgctca tgtgctgcac gctgccggtg ctgctgtcga cgcagcgttg cggtgcgacc
     1441 cacaactcga tgcgctggtt gttggcttcg ctgctcaggt tgaagcggcc gtcgcccagt
     1501 tgttcttcga cataaggcac cgccagcgcg ggttggcctt ctcggtcgat gaccatgccc
     1561 ttgccgctga ctttgacgtt ccactcaggc gtgtggccgg cggcacgcag gatcaacagc
     1621 ttgaagttcg ggtcatcgca tgcggtgccg gagcgctcga cgcgatacaa ctggccgagg
     1681 tcgagccggt cgccggcgat ttttccgcgc acgtcggcaa acagcttgcc ttgctgatcg
     1741 gcgagggtcg cggcctcttg cagcacgctg gtgccgccga tgtcgttgac cacgtattga
     1801 cgttgttcgc cacacggctg aaacaccagt tcgccgtcgg ccgccgtcaa ttgcccttgc
     1861 atgcgggttt gcccgacatg ggcagcgctt tcacgcgggc catcaagcaa ttggcaggcg
     1921 gcgaacaacg gcagcagggc aacaacgatc aacgaacggg caacacgcat cttcggctct
     1981 ccagacaagt gccgccacgt tacgcaggct gacaatcaaa tgcaaaagat cgcagccttc
     2041 aacccacgtg aaaggtctgg ccggtttgca ggccttcgac acttttcgcg taggccaacg
     2101 ccacatccgc cgcaggtacc ggcttgtagc cacgaaagta cggggcatag ctgcccatgg
     2161 cttcctgcag cacggtcggg ctgatcgagt tcacccgcag accgcgcggc agttcgatcg
     2221 cggcagcctt cacgaagctg tccagtgcac cgttgaccag tgccgccgag gcgccagagc
     2281 ggatcgggtc gtggctgagc acgccggtgg tgaaggtgaa cgaggcgcca tcgttggcgt
     2341 attcgcgacc gatcagcagc aggttgacct ggcccatcag cttgtctttc aggcccaggg
     2401 caaagctgtc ggcgttcatc tcgcccagcg gggcgaaggt tacgttgccg gccgcgcaga
     2461 ccagcgcgtc gaacttgccg gtttgctcga acagttggcg aatcgaggcg ctgtcgctga
     2521 tgtcgacccg caagtcgccg ctgctgcgac cgatgcgaat cacttcgtgg cgctggctca
     2581 gttctttgtc cacggccgaa ccgatggtgc cggcggcacc gatcaacaga atcttcatgg
     2641 gctgttcctc aatgggttga acgaggtttc agtctagagt ggttttttct gcggataagc
     2701 gcactaatag gcaacctttg gttttcaaat ggaaacaatc catgagcgaa atggatgatc
     2761 tggcggcgtt tgcggtgttg atcgaggcgg gcagttttac cctggcggcg cagcaattgg
     2821 gctgcagcaa gggccagttg tccaagcgca tcagccagtt ggaagcgcag ttttgcgtgg
     2881 tattgctgca acgcaccacc cgccgtttga gcctgacggc ggcgggtgcc gcattgttgc
     2941 cgcaagccca ggcgctggtg gtgcaggtgg agcgggcgcg tcaggcgctg gcgcgcttga
     3001 aggatgacat ggccgggccg gtgcggatga cggtgccggt atcgctgggg gaaaccttct
     3061 tcgacggatt gctgctggag ttttcgcaac aataccccga ggtgcagatc gagctggagc
     3121 tgaataatca ctatcgcgat ctttcacggg atggcttcga tctggcaatc cgcaccgagg
     3181 tggccaatga cgagcgcctg gtggccaaac ccttgctggc ctggcaggaa atgacctgcg
     3241 ccagcccggc ctatcttgca cagtttggcg agccgctgac tccgcaagag ctggtcgaac
     3301 accgttgcct gctcaacagt cactacagcg gtcgcgaaga gtggctgtat caccaacagc
     3361 atgaactgct cagggtacgg gtttccggtc cgttcgccag caaccactac aacctgctga
     3421 agaaagccgc tctggccggc gccggcatcg cccgcctgcc gtcgtacctg ttgcagacgg
     3481 aactggctga cgggcgtttg tgctggttgt tgcgggacta tcagacccga cgcatgccga
     3541 tgtacctggt gcatccgtat cagggtggtt tgcccaaacg cacgcaagta ctggcggatt
     3601 atctgattgg ctggttcaag cgcagtggcg aggcgctgga tcgccttcag cgctaaccgt
     3661 ccttgttaac ccattccctg taggaggagc tgccgaaggc tgcgatcttt tgatttggtt
     3721 ttttacaagc cagatcaaaa gatcgcagcc ttcggcagct cctacattga aatcagcggt
     3781 tgtagtgtct ggcaatcaga tgatcgatcg acaatttccc cggcccggtc gccatcaggt
     3841 acagcagcac cgccgcccag gtgccgtgag tcggataggc atccgggtac acgaacagct
     3901 gaatagtcag ggtcatgccc agcagcgcca gtgcggaaaa ccgtgtggcg aagccgatca
     3961 ggatcagcac cggaaagaag tgctcggcga acgctgccag gtgtgcggcg atttccggtg
     4021 acagcagcgg cacgtggtac tcgcttttga acagtggaat ggtcgagtcc gccaagtgtg
     4081 gccagccgag ttgagcggtg ccgtcgatca ggtcaatggc caggccttcg accttggtct
     4141 gcccggactt ccagaacacc gcggcaatcg aaaaacgcgc gatgaaagcg atcaggctat
     4201 gggggatttt ctccagcagc gcaatggcgt gggcgatgag gttgttcatg gcgattcctt
     4261 gtcattcagg tcaacgatgg cgtggtgcgc aatcagcagc gcgagggtgt ggctgaggtc
     4321 gaatggcggg ctgttttgtg cggctgccaa cagcggctgg ccatcgagca ggtgacgcag
     4381 aaacgcgctg gcgccgggtt cgagggccaa cacctcgacg tcgagaccgt tgcgcaacac
     4441 caaggcgtgt tgtccgtggt ggatgtcgat gccggccagc gttgcatcac cctgatgcgc
     4501 ggcccagatc gcgaccacgg cgtggttcga atccagcagg tgcagggacg gatgaagctt
     4561 gatgcgcagc tcgctcagcc catcgggctc ggcgagtgct gcggtgatct gttgcgggtg
     4621 caccggcagc gcatcggcgg cgtgataggc acaggtgcgc aggcgttcca gacgtgccac
     4681 atcggccaga tacggcacgc tcgcggccgg ttcgaaggcg tcgatgaagg ctggcagcgc
     4741 ttcgccataa cgactcatca acgggctgtg tggtggttgc tgttggacga aaacggcagc
     4801 catggcgcgg aagaactcct cgccgaccaa ttgctgcacc accggataac tgtccgccaa
     4861 ggcattgatc aatgaaccct gcacattgtt gcggtacacc gaaaatcggc tggccggatc
     4921 ggcaccgttg gcgcagcaca aaccctcggg gcagggccgt tgcgggtcga gcagggcggc
     4981 agcaaaagcc gcttgagtgc tcatgaccgg actcccgcgt cgagcaatat ttcgtcggcc
     5041 tgccgtgcct cggccagcaa cacattgaac gctggcagtt gattgtcgcg ctcgatcaga
     5101 gtcgccaccg cgccggtgcg gtgcagcgct tgccggtaca aggcccacac cgcttgatcg
     5161 atcggcgcac cgtgatcgtc gatgagcaag cgctcgccga ggctgtcgtt gtcttcggca
     5221 aatccggcca gatgaatttc gcccacggcc tgcaacggca gggcgtcgag gtaagccagt
     5281 ggatcgcgtt gatgattgat gcacgagacg tagacgttat tgacgtccag cagcagaccg
     5341 caaccgctgc gccgcaccac ttcggcgatg aagtccggct catcgatggt cgagcgctgg
     5401 aacgccagat aggtcgccgg gttttccagc agcatcgggc gcttgagggt gttctgcacc
     5461 tggtcgatgt gcgcgcagac ccggttcagg gtcggcatgt cataggccag cggcagcagg
     5521 tcattgagaa acaccgggcc gtggctcgac caggccaggt gttcggaaaa ggattgcggt
     5581 tgataacgct cgatcagctg cttcaggcgt tgcaggtgct gaacgtccag tggcccttcg
     5641 gccccgatgg acagtccgac gccgtgcagt gacagcggat actgctcgcg gatcaaaccg
     5701 agaaagtgat gaaacggacc gccgtccacc atgtagtttt cggcgtggac ttcgaagaaa
     5761 ccgagatccg gtagcgaacc gagcacttca cgaaagtgcc cggtcttgag ccccagcccg
     5821 gcacggcgcg ggagcccggg gagggctgcc tgagtgcggg tcgggacagg ttcgtcgagg
     5881 gtgaacatga tcgtcactca ggctggccgt ggatcaggac ttggctttga acgcttgcat
     5941 ctgaccgaaa ccggtcggcg aggtcttgct ctcggtggtg gcacaggtgc ctttcggcac
     6001 gagtttccaa gcgttggcct ggtagtccat tttcgacgta ccggcgcagg tggtgcctgc
     6061 accggcggcg caatcgttgt gacctttcat ggccacgccg aagcatttct ccatgtcggc
     6121 atcagcggcg tggacggttg atatggcggc catgctcagg gcagagccaa gggccagaac
     6181 cagggcggta gcggacaggg tgcgggtggt agcagtcatg atgtttctcc aggtttgagc
     6241 ttgggttttg caagcgaagt gcgcttgctt accccactag agaaagctca tggcgatgcg
     6301 ttacagccga cccgaaactt ttttagaaat ttttcagaac cctgtattcc ccctgtggga
     6361 gcgagcctgc tcgcgaaggc gtagtgtcag acgccattga tgttcaatga cccaccgcat
     6421 tcgcgagcag gctcgctccc ccagtgattg tgttcactcc ctcctgttct atagtcattt
     6481 caacggccac gttgacaggc cgatttacca ggacggtatc tcatgacgaa ggatcgctca
     6541 gactctgctg cgctatcgcg caaggtttca tcgtttgctc tggcctgcac gctgcccctg
     6601 ttgctctcca cggttcacgc ccagccacac cccgatccac tggacacgct cacgcgcatc
     6661 aaccacaact acaacactct caaggacaac tgccaggaac ccgacaccgg cgccgcgcgc
     6721 gggctgtatt actgcagtgg cgtgaccttg cgcatggtca acgacgggcc gttcaatccg
     6781 tgggattaca gcccctacgc gatcaagatt ggtgcgacgt cctattcgtg gatccgcaag
     6841 gacctgagca cgcggattct catccacccg gccgggttca tcctgcgcac gcccaccgat
     6901 gcggcggcgc tgaagctgcc ggtcaaggag cagggcttta cctgcatcta tgccttcgat
     6961 ggcggcaccg ggccggagcg caagtggtac ggctgcggtt tctttgacag ccgcgaaccg
     7021 ccgcgcgctg cgcaaggcac gatgaacaac cgcaatgccg cgctggccta tggcacctgt
     7081 gccgaggccg gggtgaccac cgccgagcaa tggacgcaga aatacaccgg tctgatgaaa
     7141 gggccgatcc agtacggtca gtgctcgtgg aacgcggaaa aacccggcga ctggcaggcg
     7201 atgatccggg tgcacgagtc gcgcccgaac acgaccaaca aagacccctt tgccttcagt
     7261 gcccaggtca acgaattcat gttgaagaac gccaccgcga ccaacgacgg cagcgaaaac
     7321 atgaagtaca tcgacgcctt tatctacaac gtcaacagca cgcagaactt cgccacgcgc
     7381 ggtgatcagg ctccgccgaa accggagaac ggcctgaaca gcgcgcgcaa tttccagaag
     7441 aaactcaagg cccagggcta cagcgtgccg atcctgcgcc tggatttcac ccaacctgcc
     7501 gagcggcgtt tcagttatgt cgccgccgat caggcaatcg acgagatggt cgtggctggc
     7561 gccccggcgc ccaagtacat cgcggatgcc gtgtggatcg agcgttacga cccgggcacc
     7621 cggaaaaacg aatggtcgct caaggtcacg ccgacggcgc agggcaaggc ggctcaggcc
     7681 actgaccaag aggccgtgta caaggaattg ttggccctgc gtggcactga cagccagtgg
     7741 cgcgacaacg agaaagcggc gggcagcatg cgtcagcaac tgagctgtct ggtgcagaac
     7801 tatccggtca agaccgagtg gaatctggag ccgttccgcc ccgtggtcag cccgcaggac
     7861 gcggcgaagg ccggttgcaa tccggtgccg gtccaggccc cgcaatacat cgcctccgcc
     7921 gactggatca aacgttacga ccccggcacc cgccaggatg aatggagcct gagcatcgtg
     7981 cccaccgcga cggggcgcgc cttgcccaac gatcaggccg gggctttgta cgatcagttg
     8041 ttcgcgctca agggcgcgga cagcaactgg cgtgacaacg aaaaatccgc cggcagcatg
     8101 cgccagcaat tgagctgcgt gctggtcaat taccgtaaca agacgccatg gaatctggag
     8161 ccgttcaggc cggcgttgtc ggacagcgaa actcgcgccg cgggttgcaa cccggtcccg
     8221 cgttgagtgt gtgtgttggc taatcaagga gatgcaagcc atgaagaaaa gtctgatcag
     8281 gaccgttcca ctgctgttgg taccgctgct gttgcacccc gcctgggcca atgccgagtc
     8341 atgtgccgag acgctgaaaa aggtcgaaac gttgtacaac aacaccgtcg acagttgcgg
     8401 gcaggacccc gcctcggact gctcggggct actggtgcgc ggcacgcacc gggctgatcc
     8461 ggccaagggg cagaaatggg acgtgtggaa tcccagcccg aaagccgtgg agctggggac
     8521 gtttgccgcc tcctacatgc gggccgacgg catcagttat gaagaccccg gcatgagcac
     8581 gcagaacggt tatctgatta cccctcggga tctggtgcgc gacccggaaa caccggtgca
     8641 cgtctattgt gcgtttccca acgatgcctg gaccgacttt cgcaacgacc gtggctgtgg
     8701 cgacaacaaa aacaccgccc cgaccgaggc ggtctgccag gccatgaagc cgcctgtttc
     8761 gacgccgaat gcctgggtcg cgcactttac ccagtacaac aacaatcggc agcaggacca
     8821 gttgcagtgt ggcttcaaca tgcgcaatcc gatgagcagc aaggaacggg tcgacgcctt
     8881 tcgcaacttc atgggcgcgc gcaaagtcat caacagtcgt gagtttcaaa ctcagaccga
     8941 actgcgcctg ggcaatccga aaaccgatga actgccggtg ctggcgttct tctacagcga
     9001 tcagcgcggc ctgaacgacg cgctggccaa tcaaaaggac tacaaggcca agaccggcaa
     9061 ggaccgcaat atcatcaaga tcgacttccc gagaactccg gtcgccaagg cctcgttctc
     9121 gtgcatccag acctcgacac cgactgaacc gaagttctgc gacaagtaca tcgagtccag
     9181 cacctgggta caacgtcccg atccgaaact ggggccgaac acctggtcgc tcacggtagt
     9241 gccgaccgcg tgcggccgcg cgatcaagga tgaccagacc gaccggatgt ttgccgagct
     9301 gtacaacaag cacaaggacg atcagcagtg gcgcgagtac agcgtcaatg gcggctcgtt
     9361 gcgtcggcag atggtctgcc acctggcggc ggtctacgac ggcaagcctg tgaggaacaa
     9421 gcctgagtgg aaccttgagc cggcacggcc ttatgtcgat caggcgacgg cggtggcgca
     9481 gcactgcaat ccgtattaat tccctgtagg agtgagcctg ctcgcgatgg cgtcgtgtcc
     9541 gacacagtga tgttgaatga cacaccgata tcgcgagcag gctcgctccc acaggggatc
     9601 ggtgttgaat tcaaaataca aaaaaacggc ctgcgaaggc cgtttttttg ttcagcgaaa
     9661 agcatcaacc gcccagatac gcctcgcgca ctttcgggtc ggtcagtagc gcttcaccgg
     9721 tgccttgcat caccacacgg ccgttctcca gaacgtacgc acggtcagcg attttcaagg
     9781 cctggttggc gttctgctcg accaggaaca ccgtcacacc gtccttgcgc agttgttcga
     9841 tgatgtcgaa gatctgctgg atgatgatcg gtgccaggcc cagcgacggc tcgtcgagca
     9901 gcagcagttt cggcttgctc atcagcgccc ggccgatggc gagcatctgc tgttcgccgc
     9961 cggacatggt gccgccgcgc tggttgaagc gttctttgag gcgcgggaac aggccgagga
    10021 ccttgtccat ctgcgcctga tagtcgccct tgtcggtgaa gaaaccgccc atggacaggt
    10081 tctcttcgac ggtcagacgg gcaaacaccc gacgaccttc cggcaccacc gcgatgcttt
    10141 tacgcatgat ctgcgacgag tcctggccga ccagttcctc acccatgtag cggatgctgc
    10201 cgctgtgcgc ctgcggtgaa ccgcagagcg tcatcagcag cgtggacttg ccggcaccgt
    10261 tggcgccgat cagggtcacg atctcgccct gacggacttc gacgttgacg ctgtgcaggg
    10321 cctggatctt gccgtagaag gtggaaacgt tttcgaactg cagcatttac gcttccccca
    10381 ggtaggcttt gatcacttca ggattgtcgc ggatctgatc cggcgtgccg tcggccagag
    10441 gtgtgccctg gttgatcacg acgatgtggt cggaaatgct catgaccagt ttcatgtcgt
    10501 gttcgatcag cagcacggtc acgttgtgct cctcacgcag cacgctgatc agcgccttga
    10561 ggtcttcggt ttccttcggg ttcagaccgg ctgccggttc gtcgagcatg aggatccgtg
    10621 ggcgggtcat catgcaacgc gcgatttcca gacgccgttg ctgaccgtag gccagggtgc
    10681 cggcggtacg gttggcgaat tctttgaggt tgaccttttc cagccagtac tcggcgaatt
    10741 ccatcgcctc gcgctcgctt ttgcggaacg ccggggtttt gaacaggccg gacaggaagt
    10801 tggtgttcag gtgacggtgc tgggcgatca acaggttctc gaccgccgtc atgtccttga
    10861 acaaccgcac gttctggaag gtgcgcac
//
