LOCUS       NZ_JAHHZH010000003     12973 bp    DNA     linear   CON 04-JUL-2024
DEFINITION  Pseudomonas sp. MG-9 NODE_3_length_651067_cov_67.7801, whole genome
            shotgun sequence.
ACCESSION   NZ_JAHHZH010000003
VERSION     NZ_JAHHZH010000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Pseudomonas sp. MG-9
  ORGANISM  Pseudomonas sp. MG-9
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 651067)
  AUTHORS   Lutfullin,M.T., Pudova,D.S., Lutfullina,G.F., Vologin,S.G.,
            Gogoleva,N.E., Shagimardanova,E.I., Sharipova,M.R. and
            Mardanova,A.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) Microbiology, Kazan Federal University, 18
            Kremlyovskaya, Kazan, Tatarstan Republic 420008, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2021
            Assembly Method                   :: SPAdes v. v. 3.12
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018729515.1-RS_2024_07_04
            Annotation Date                   :: 07/04/2024 03:27:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 89
            rRNAs                             :: 8, 9, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 6, 8 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 25 of 68
            Pseudo Genes (incomplete)         :: 48 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:31:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 179731
            Orig. end                         :: 192704
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHHZH010000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12973
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="1"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12973
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12973
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7973](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7973)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    1428..1441
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.83"
                     /tool="antismash"
     misc_feature    1593..1602
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.44"
                     /tool="antismash"
     misc_feature    complement(1978..1992)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.5"
                     /tool="antismash"
     gene            2283..4091
                     /locus_tag="KKQ10_RS09100"
                     /old_locus_tag="KKQ10_09100"
     CDS             2283..4091
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /GO_process="GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1117:
                     sodium/hydrogen exchanger"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003223951.1"
                     /locus_tag="KKQ10_RS09100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_09100"
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="WP_127650957.1"
                     /transl_table=11
                     /translation="MPHEGNLLQAAVVFLFAAVLTVPLAKRLQLGAVLGYLLAGVIIGP
                     SVLGLIGNPQSVAHISELGVVLLLFIIGLELSPRRLWVMRKSVFGVGLAQVLLTAAVIG
                     VLALYVFGQPLNSAIVLGLGLALSSTAFGLQSLAERKELTSPHGRLAFAILLFQDIAAI
                     PLIALVPMLAGGAHDTSSAGSLNHGLQVLGGIAVVVVGGRYLLRPVFRVVAKTGLPEVS
                     TATALLVVIGTAWLMDLVGVSMALGAFLAGLLLADSEYRHELEAQIEPFKGLLLGLFFI
                     SVGMGANLSLLLSAPITVLGLTLLLIGLKLPLLFVVGRLAGGLNKVSAIRLGIVLAAGG
                     EFAFVVFKIGRDQGLFEPRLYDLLVLTITLSMALTPLLLLICARLVSPKVQPVEVPEKF
                     REIDTEAPRVVIAGMGRMGQIVARILRAQNIKFVALDTSVETIELSRSFGGVPVFYGDP
                     MRPEILNAAKVGEAEYFVIATDDPETNIKTAEIVRKLYPHMKIIARARNRQHVHRLVDV
                     GADPIRETYYSSLEMSRRTLVGLGLTQAQADARIKRFKHHDEQVLEAQHAVYDDAAKVL
                     QTAQEARAELARLFESDQLEEESRQS"
     gene            complement(5001..6260)
                     /gene="hcnC"
                     /locus_tag="KKQ10_RS09105"
                     /old_locus_tag="KKQ10_09105"
     CDS             complement(5001..6260)
                     /EC_number="1.4.99.5"
                     /codon_start=1
                     /gene="hcnC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007965624.1"
                     /locus_tag="KKQ10_RS09105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_09105"
                     /product="cyanide-forming glycine dehydrogenase subunit
                     HcnC"
                     /protein_id="WP_215500889.1"
                     /sec_met_domain="DAO (E-value: 6.5e-65, bitscore: 215.0,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKFYDVIIAGGGVIGASCAYQLSKRQGLKVALIDAKRPGNATRA
                     SAGGLWAIGESVGLGCGVIFFRMMSANRKRETQGAAVAVDASTPHILPESFFDFALQSN
                     ALYPALHRELKDNHGMDFKFEKTGLKFVIYDDEDRLYAEHIVGCIPHLADQVRWLDQAA
                     LRESEPSVSHEARGALEFLCDHQVSPFRLADAYMEGARQNGVDIFVNTNVTGVLHHGSR
                     VTGVQTAEQGVFHCKTLINAGGAWAADLSEWATGIRIPVKPVKGQILLTERMPKILNGC
                     LTTSDCYVAQKDNGEILIGSTTEDKGFDVTTTYPEIAGLVQGAVRCLPELKDVNLKRTW
                     AGLRPGSPDELPILGPMRGVEGYLNACGHFRTGILTSAITGVLLDKLVNDEPLPLDITP
                     FLADRFETASVKQERELELA"
     gene            complement(6253..7662)
                     /gene="hcnB"
                     /locus_tag="KKQ10_RS09110"
                     /old_locus_tag="KKQ10_09110"
     CDS             complement(6253..7662)
                     /EC_number="1.4.99.5"
                     /codon_start=1
                     /gene="hcnB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907901.1"
                     /locus_tag="KKQ10_RS09110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_09110"
                     /product="cyanide-forming glycine dehydrogenase subunit
                     HcnB"
                     /protein_id="WP_127650959.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 3.7e-21, bitscore:
                     70.6, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 6.4e-10, bitscore:
                     34.4, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQQPVIVGGGPAGMAAAIELARHGVRCTVLEEASRLGGVVYRGP
                     LRDGVQLDYLGPRYSEALGKLHGDFQEQAGLIDVRLNHRVVGAEGTRALVVLDGDEQLH
                     EIHYPQLLLAAGCHERSVPFPGWTLPGVIMLGGLQLQIKSGVVKPQGPVIIAGTGPLLP
                     LVATQLHASGVKVAGVYEACAFGKIARESLALLNKPQLFLDGLSMLAYLKLHGIPMNYG
                     WGVVEAHGDGELKSVTVAPYSATWEPDLTRIERFEAQTLAVGYGFIPRTQLSQQMGLDH
                     GFSDDGYLRANSNTWQQSNEPHVHLAGDMGGIRGGEAAMLAGKIAATSILLQRGIIEED
                     LARVRRDRFLSKLKTIVRFRAAVDRYTERGVGQTALPAADTVICRCEHATRADIDRALE
                     QGVQDIASLKMRTRVSMGDCQGRMCVGYCSDRLRQATGRKDVGWLRPRFPIDPIPFSAF
                     QSVGTEAASHE"
     gene            complement(7659..7973)
                     /gene="hcnA"
                     /locus_tag="KKQ10_RS09115"
                     /old_locus_tag="KKQ10_09115"
     CDS             complement(7659..7973)
                     /EC_number="1.4.99.5"
                     /codon_start=1
                     /gene="hcnA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070675.1"
                     /locus_tag="KKQ10_RS09115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_09115"
                     /product="cyanide-forming glycine dehydrogenase subunit
                     HcnA"
                     /protein_id="WP_215500890.1"
                     /sec_met_domain="Fer2_4 (E-value: 2.6e-22, bitscore: 73.8,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHCLERTFDIQPLTQADMTVHINGQPVTAAIGETVLSVIQSLGVR
                     QVARNDHDQISGAYCGMGVCQCCLVKINGRHKRRACQTVVRDGMQIETRVNRINEQEVV
                     "
     misc_feature    8213..8227
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.5"
                     /tool="antismash"
     gene            complement(8216..8755)
                     /locus_tag="KKQ10_RS09120"
                     /old_locus_tag="KKQ10_09120"
     CDS             complement(8216..8755)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003223943.1"
                     /locus_tag="KKQ10_RS09120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_09120"
                     /product="hypothetical protein"
                     /protein_id="WP_215500891.1"
                     /transl_table=11
                     /translation="MDKKEIKRQAMQMLEAGRSKTETFKALSGGAVKDKVLAYWIGSRP
                     DPQLLERHAGKIKVLLGLIYVQALIGLVGGYLLGLTISDGLAFVLGLLAGSVPLLFAWG
                     FHRHSAQTYTVYMLLSISQMPRLFKDYAEDPVSVIIGVVTTLAMVFFVAWVKNLLFPDL
                     GFMGSKKIKGQFVFSN"
     gene            complement(9015..9947)
                     /locus_tag="KKQ10_RS09125"
                     /old_locus_tag="KKQ10_09125"
     CDS             complement(9015..9947)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914999.1"
                     /locus_tag="KKQ10_RS09125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_09125"
                     /product="hypothetical protein"
                     /protein_id="WP_215500892.1"
                     /transl_table=11
                     /translation="MNRSSDGFYPAFEGESSNVSGVSWGAIFAGAAAAAALSMILVLLG
                     FGLGFSAVSPWAGEGVSAKSLGISTIVWLALTQIIASGLGGYIAGRLRVKWANMHGDEV
                     YFRDTAHGFLAWCVATLVTAVLVVGSVSSVISGGVQAGASVAGGAAAAATQAAGTAAAN
                     TNSDQYGYFVDSLFRDDRPASVSDDAAHGAVTRIFAQSLANGQLSPEDRTYLAQLVAQR
                     TNLSQADAERRVDEVYARTQKAIADAKVKARQATDTAAKVAAWTSLWMFIALLAGAFFA
                     SLAATFGGRRRDAVEYVEVQTYATTTTVR"
     misc_feature    10081..10093
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: strong, score: 25.04"
                     /tool="antismash"
     gene            10255..10938
                     /locus_tag="KKQ10_RS09130"
                     /old_locus_tag="KKQ10_09130"
     CDS             10255..10938
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934994.1"
                     /locus_tag="KKQ10_RS09130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_09130"
                     /product="response regulator transcription factor"
                     /protein_id="WP_127650964.1"
                     /transl_table=11
                     /translation="MTRILTIEDDAVTAREIVAELSSHGLEVDWVDNGREGLDRAVSGN
                     YDLITLDRMLPELDGLAIVTTLRTMGVATPILMISALSDVDERVRGLRAGGDDYLTKPF
                     ATDEMAARVEVLLRRQNSVATQATTLQVADLELDLISHEARRAEQVLTLLPTEYKLLEF
                     LMRNSGQILSRMMIFEEVWGYHFDPGTNLIDVHIGRLRKKIDAAGHVPLIRTVRGSGYV
                     IAEPV"
     gene            10916..12310
                     /locus_tag="KKQ10_RS09135"
                     /old_locus_tag="KKQ10_09135"
     CDS             10916..12310
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018928976.1"
                     /locus_tag="KKQ10_RS09135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_09135"
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_127650965.1"
                     /transl_table=11
                     /translation="MSLPNPSDGWRSSSSRLLALYSSLFVAWSAILMGVMYFEVSSYLD
                     NLAKHSLMQRQHLFSHFRGEQLEEALAASMTFDIRGIDAYGLFDARGVYLGGALQQLPE
                     GLPLDGKIHTLDDCADSDDPTLPADSCDAVATQTRDGRWLVLLRDNGSLFAVTRIILHA
                     LFWGVSLTILPGIAGWHLLRRRPMRRIRAIQDSAQAIVAGDLTHRLPLSNRRDELDMLA
                     AIVNAMLDRIERLMHEVKGVCDNIAHDLRTPLTRLRAQLYRMQQQAGEGSQEAAQLDLV
                     LAEADTLMARFRGLLRISELEDRQRRSGFVELDPVKLLEELHEFYLPLAEEGELLFVLN
                     MPETLPPLNGDRALLFEALANLLSNSIKFTPAGGTVILRGVNDGGHTRIEVHDSGPGIP
                     ETEREAVFQRFYRAEGGQPQSGFGLGLSIVAAIVSLHGFNLEVGSSELGGAKLVLDCRQ
                     RLIANS"
     gene            complement(12434..12844)
                     /locus_tag="KKQ10_RS09140"
                     /old_locus_tag="KKQ10_09140"
     CDS             complement(12434..12844)
                     /NRPS_PKS="Domain: Polyketide_cyc2 (0-132). E-value:
                     9.2e-31. Score: 99.1. Matches aSDomain:
                     nrpspksdomains_KKQ10_RS09140_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016986224.1"
                     /locus_tag="KKQ10_RS09140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_09140"
                     /product="SRPBCC family protein"
                     /protein_id="WP_127650966.1"
                     /transl_table=11
                     /translation="MATASATIDIPASADQVWQLIGGFNTLPDWLPLIAKSELSEGGRV
                     RTLQTADGGVVIERLQAFDNAARTYSYSIEQAPFPATDYLATIKVEAHGQGARVTWSGR
                     FSAKGVSDEEVVALFSGIYQGGLEALRANYPT"
     aSDomain        complement(12449..12844)
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KKQ10_RS09140_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="9.20E-31"
                     /label="KKQ10_RS09140_Polyketide_cyc2.1"
                     /locus_tag="KKQ10_RS09140"
                     /protein_end="132"
                     /protein_start="0"
                     /score="99.1"
                     /tool="antismash"
                     /translation="MATASATIDIPASADQVWQLIGGFNTLPDWLPLIAKSELSEGGRV
                     RTLQTADGGVVIERLQAFDNAARTYSYSIEQAPFPATDYLATIKVEAHGQGARVTWSGR
                     FSAKGVSDEEVVALFSGIYQGGLEALRA"
     misc_feature    12838..12854
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 19.52"
                     /tool="antismash"
ORIGIN
        1 ctgcgttgta gtacctctgt ttgagctgtt cttcgacgat cgcctgcttc tcttcgatga
       61 tcagcacctt gccggcgcgc tgggtccagg cctccagcac cggtgacagc ggatagacca
      121 ggttgatctt cagcagcgca acgccggcgt tttccagttg ttcaagggag atgccgcatt
      181 gctgcaaggc ctggcgcaca tcgccataag ccttgccgac tgctgcgatg accagcgccg
      241 gttgcgcagg cgcagtgacg gcggtgtcga tcgggttggc cagggcaaac acccgggcgg
      301 cgtcgaactt gaacgcggtc cggcgctcga tctgcatgcc cggcaggtcc ggccagcgcc
      361 aatgcaggcc gtccgggccc ggatcgatct gcggggtgac gaactcggga agcgggatcg
      421 actcgacaga actggtggcc tcgacggtct cggtgatcgc cttgaaaccc acccataacc
      481 cggaggcccg ggataacgcc cagccccaca ggccgcagcg ttcataatcg gccagccccg
      541 ccgggtgaat caccggcaag ccccaggcaa tcagcgtgcg gtcggactga tgcgacatcg
      601 atgacgatac acacccgtga tcgtcgccgg tgaccaccag cacgccgccg ttgggcgacg
      661 aaccatagga actgccgtgt ttgatcgcgt caccggaacg gtcgaccccc ggccccttgc
      721 cgtaccacat gccgaacacg ccgtcataca aacgctgcgg atcgctttcc acacgctggg
      781 tgccgatcag ggctgtggcc gccaggtctt cgttgatcgc cggcagaaag cgaatgttcg
      841 cgttctccag gacggccttg gccttgacca gctcgcggtc cacgccgccc agcggcgagc
      901 cgcgataacc actgacgaat cccgccgtgc gaacgccgtt ggatcgatca agttcagcct
      961 gcgacagcag caatctcacc agcgcctgct gaccgctcaa aaagatccgt tcgttgggct
     1021 gcgcgtgttc ccggcaatac atcgagcgac tgtcatattg ctcgattcgc gccaataccg
     1081 cgtgagcttc atcagtcgcc aatttgtcgg gttgcaggcg ttgatgttct gaatcaatgg
     1141 tatccaagta cacagtttca aatccttatg aaaacttcac attaaatatg cttatttgcg
     1201 cactacagca gaggttgcat caaaattttc gaccgctgaa cgacggaaac tcgggggaaa
     1261 caagtacctg cgggccacct ggcaagacac actcatacgg ctccgcgatt tctcgggagc
     1321 acatcaagtt aattcagtct ttttaaacag acagcaagaa atccaggtac aaattttcaa
     1381 tcccttatga aaactttact ttaaatagat gtactttctc gcctcggcga acataacttc
     1441 agactggcga attcgctcag gcgagagtga cgcttttata agcgtgcgac ggctcgcggc
     1501 gatccggttt aactgaagtt gctgaggtta tgaaccgttc aagaaggcat cacctgcggc
     1561 ttgaagggca agctgctgca cagggacctt tcggtttttt gaaatgatga acccggctga
     1621 atccgcgctt ctccgggact ctgccaagtg attgtcatga atttggcgac gtgacaatga
     1681 cgccacgatg tcagtctgtg cacgatcatg tgccacaccc tggaaaaaac aggaaatgag
     1741 ctgatcgagc tgaaatcgca ggggttccga tccaatggaa tccagataca agtttcaatt
     1801 ccttatgaaa acttcgcgcg gccatcctgg tcgctttcat tacggccgtt ataaacagcc
     1861 agcatcttgg gtgaaaaata gtaggttcgg agcccttgcc ttgtcaatcc ttgtaacaaa
     1921 catttaatgg taaatcgcat gccatgaact atctggtaca cgcaaccctc catcgaactc
     1981 tcacattggg aacttcgtcc atcgccagcg attatcgagt tatcaggcaa ttgctcggca
     2041 atatcaccgc cacatcaccc aagacaaaag tataacttca catcgcccgt gaactgtcaa
     2101 agcatccgta attcgatgat ggcaatgcga caacttttaa gttgcctaac gttattggca
     2161 taaaacattc accgatctga aaaggtcctg gtggaaaatc gctgccccga gcgcctgtgg
     2221 tgtgacaggg aatcagcctt tatccttgac gcccccgccg taccgctgtc aggagtaaac
     2281 ccatgcccca tgaaggcaac ctgttacaag ccgctgtcgt gtttctgttc gccgcggtgc
     2341 tcaccgtgcc cttggccaaa cgtctgcaat tgggcgccgt gctcggttat ctgctggcgg
     2401 gggtgatcat cggcccgtcg gtgcttggcc tgatcggcaa tccgcaaagc gttgcgcaca
     2461 tctcggaact gggtgtggtg ttgctgctgt tcatcatcgg ccttgaactg tcgccacgtc
     2521 ggttgtgggt gatgcgcaaa tcggtgttcg gtgtcgggct ggcgcaggtg ctgctgaccg
     2581 ctgcggtgat cggcgtgctg gcgttgtacg tgttcggtca gccgctcaac agtgccattg
     2641 tgctcggcct gggcctggcg ctgtcgtcca ccgcattcgg cctgcaaagc ctggccgaac
     2701 gcaaggaact gaccagccct catgggcgac tggcgtttgc gattctgctg tttcaggaca
     2761 tcgcggcgat cccgttgatc gcgctggtgc cgatgctcgc cggtggcgct cacgacacca
     2821 gcagcgcggg cagcctgaat catggtttgc aggtactcgg cgggatcgcc gtggtggtgg
     2881 tcggcgggcg ttatctgttg cgtccggtgt ttcgcgtggt ggcaaaaacc ggtctgccgg
     2941 aggtgtccac cgccaccgca ttgctggtgg tgatcggcac cgcgtggctg atggatctgg
     3001 tcggggtgtc gatggcactc ggggcgtttc tcgccggcct gctgctggcg gattcggaat
     3061 atcgccatga actggaagcg cagatcgaac cgttcaaagg cctgctgctg gggctgtttt
     3121 tcatcagcgt cggcatgggc gcgaacctga gcctgctgct gagtgcaccg atcaccgtgc
     3181 tgggcctgac gctgttgctg atcggtctga agttgccgct gctgtttgtg gtcgggcgtc
     3241 tggccggcgg gctgaataaa gtcagtgcga ttcgcctggg catcgtgctc gcggccggtg
     3301 gtgaatttgc ctttgtggtg ttcaagatcg gtcgcgatca gggcctgttc gaaccgcgtc
     3361 tgtatgacct gctggtgctg accatcaccc tgtcgatggc gttgacgccg ttgctgctgt
     3421 tgatctgtgc gcgactggtc agcccgaaag tgcagccggt ggaggtcccg gagaaattcc
     3481 gcgaaatcga caccgaggcg ccgcgagtgg tgatcgccgg catgggccgg atgggccaga
     3541 tcgtggcgcg gattctgcgg gcgcagaaca tcaagttcgt ggcgctggac acctcggtgg
     3601 aaaccatcga actgtcgcgc agcttcggcg gtgtgccggt gttttacggc gacccgatgc
     3661 gcccggaaat tctcaatgcg gccaaggtcg gcgaggcgga atattttgtg attgccacgg
     3721 acgatccgga gaccaacatc aagaccgccg agatcgtgcg caagctgtat ccgcacatga
     3781 aaatcatcgc ccgggcgcgt aaccgtcagc acgtacaccg tttggtcgat gtcggcgccg
     3841 atccgattcg agagacctat tactccagtc tggaaatgag ccgccgcacg ctggtcggcc
     3901 tcggtctgac ccaggcccag gccgatgcgc ggatcaagcg cttcaaacac cacgacgaac
     3961 aggtactgga agcccagcac gcggtttacg acgacgcggc caaagtcctg cagaccgccc
     4021 aggaagcccg ggcggaactg gcgcggttgt tcgaatcgga tcaactggaa gaggaatcgc
     4081 gccagtcctg acttcagcac aattccaatt gcaggagtga gcctgctcgc gattgcaatc
     4141 tatcagcaga tacctctttc tctgaaagac cgccatcgcg agcaggctca ctcccacaag
     4201 gttcggcgta gtgcgcaaat gaccggtacg acttgactac tgtaggagtg agcctgctcg
     4261 cgattgcaat ctatcagtag atatctcttt ctctgaaaga ccgccatcgc gagcaggctc
     4321 actcctacaa ggttcggtgt agtgcgcaaa tgaccggtac gactcgacta ctgtaggagt
     4381 gagcctgctc gcgattgcaa tctatcagca gatacctctt tctctgaaag accgccatcg
     4441 cgagcaggct cactcctaca aggttcggcg tagtgcgcaa atgaccggta cgactcgact
     4501 actgtaggag tgagcctgct cgcgattgca atctattagt agatctctct ttcactgaaa
     4561 gaccgccatc gcgagcaggc tcactcccac aaggttcggt gtagtgcgca aatgaccggt
     4621 acgactcgac tactgtagga gtgagcctgc tcgcgattgc aatctataag cagatatctc
     4681 tttctctgaa agaccgccat cgcgagcagg ctcactccta caaggttcgg tgtagtgcgc
     4741 aaatgaccgg tacgactcga ctactgtagg agtgagcctg ctcgcgatgg caatctatca
     4801 gcagatacct ctttctctga aagaccgcca tcgcgagcag gctcactccc acaaggttcg
     4861 gcgtagtgcg caaatgaccg gtacgactcg actactgtag gagtgagcct gctcgcgttt
     4921 gcaatctatc agcagatacc tctttctctg aaagaccgcc atcgcgagca ggctcactcc
     4981 tacagggatt gtgggtgaga ttaagccagt tccagctcgc gctcttgctt gaccgaagca
     5041 gtctcaaacc ggtccgccag gaacggtgtg atatccagtg gcagcggctc atcattgacc
     5101 agtttgtcca gcagcacccc ggtgatcgcc gaggtcagga tgccggtgcg gaagtgtccg
     5161 caggcattca ggtaaccctc caccccacgc atcggcccga ggatcggcag ctcatccggc
     5221 gagcccgggc gcaggccggc ccaggtgcgt ttgaggttga catccttgag ctccggcaga
     5281 cagcgcaccg cgccctgcac cagcccggcg atttccgggt aggtggtggt aacgtcgaag
     5341 cctttgtctt cggtggtgct accgatcagg atctcgccgt tgtccttctg cgccacatag
     5401 caatcgctgg tggtcagaca gccgttgagg atcttcggca tgcgttcggt cagcaggatc
     5461 tggcccttga ccggtttcac cgggatacga atacccgtcg cccactcgct caagtccgcc
     5521 gcccaggcac caccggcgtt gatcagcgtc ttgcagtgga acacgccctg ctcggccgtt
     5581 tgcacaccgg tcacacggct gccgtgatgc aacacacccg tgacattggt gttgacgaag
     5641 atgtccacgc cattttgccg cgcgccttcc atgtaggcat cggcgaggcg gaacgggctg
     5701 acctggtgat cgcagagaaa ctccagcgcc ccgcgtgctt cgtggctgac actcggctct
     5761 gactcacgca atgcggcctg atcgagccag cgcacttgat cggccaggtg cggaatgcag
     5821 ccaacaatgt gctcggcgta gagccggtct tcgtcgtcat aaatgacgaa tttcaacccg
     5881 gtcttttcga acttgaaatc catcccgtga ttgtctttca gctcacggtg cagcgccggg
     5941 tacagcgcgt tggactgcaa ggcgaaatcg aagaacgact ccggcaggat gtgcggcgtg
     6001 ctagcgtcca ccgccaccgc cgcaccctgg gtttcgcgct tgcggttggc cgacatcatg
     6061 cggaagaaaa tcaccccgca acccagtccc accgactcgc cgatggccca caagccgcca
     6121 gccgaggcgc gcgttgcgtt gcccggacgc ttggcatcga tcagcgcgac cttgaggccc
     6181 tggcgcttgg acaattgata ggcgcaggac gccccgatca cgccgccacc ggcaatgatc
     6241 acgtcgtaga acttactcat gggaagcggc ctccgtgccg acggactgga acgcagaaaa
     6301 aggaatcgga tcgatcggga agcgcggacg cagccagccg acgtccttgc ggccggtggc
     6361 ctgacgcagg cggtcgctgc agtagccgac gcacatccgc ccctgacaat cgcccatgct
     6421 cacccgggtg cgcatcttca ggctggcgat gtcttgcacg ccctgctcca gcgcccgatc
     6481 gatgtcggcg cgggtggcgt gttcgcagcg gcagatcact gtgtccgcgg ccggcagcgc
     6541 ggtctggccg acgccgcgtt cggtgtagcg gtccaccgct gcacgaaagc gcacgatggt
     6601 tttcagtttg ctcaggaagc ggtcgcgacg cacccgcgcc agatcttctt cgataatccc
     6661 gcgttgcagc aggatcgagg tcgcggcgat cttgccggcg agcatcgccg cctcaccacc
     6721 gcgaatcccg cccatgtcgc cggccaggtg cacgtgcggc tcgttgcttt gctgccaggt
     6781 gttcgagttg gcgcgcaggt agccgtcgtc actgaaaccg tgatccaggc ccatctgctg
     6841 actgagctgg gtgcgcggaa tgaagccgta gccaacggcc agggtctggg cttcgaagcg
     6901 ctcgatgcgt gtcaggtccg gctcccaggt ggccgagtac ggtgccaccg tgacgctttt
     6961 cagctcgccg tcgccgtgcg cttcgaccac gccccagccg tagttcatcg ggatgccgtg
     7021 cagtttcagg taggcgagca tgctcaaacc gtcgaggaac agctgcggtt tgttcagcag
     7081 cgccagactt tcgcgggcga tcttgccgaa tgcgcaggct tcataaacgc ccgcgacttt
     7141 gacgccggac gcgtgcagct gggtcgccac cagcggcagc aacggcccgg tgccggcgat
     7201 gatcaccggc ccctgcggct tgaccacgcc acttttgatt tgcagttgca gaccgcccag
     7261 catgatcacc cccggcaacg tccagcccgg gaacggcacg ctgcgctcgt ggcatccggc
     7321 ggccaacagc aattgcgggt aatggatttc gtgcagttgc tcgtcgccat ccagcactac
     7381 cagtgcgcga gtgccttcgg cgccgaccac gcggtggttc aggcgcacgt cgatcaaccc
     7441 ggcctgttcc tggaagtcac cgtgcagttt gcccagcgcc tcggaataac gaggccccag
     7501 ataatccagc tgcacgccat cacgcagcgg cccgcgatag accaccccgc cgaggcgcga
     7561 agcttcttcg agcacggtgc aacgcacccc gtggcgcgcc agttcgatgg ccgctgccat
     7621 ccccgccgga ccgccgccga cgatcaccgg ttgctggctc atacgacctc ctgctcgttg
     7681 atgcgattga cccgggtttc aatctgcatg ccgtcgcgca ccacggtctg gcaggcgcgg
     7741 cgcttgtggc ggccgttgat tttcaccagg cagcactggc acacgcccat gccgcagtag
     7801 gctccgctga tctggtcatg atcattgcgt gcgacctggc gcacgccgag ggactggata
     7861 acgctgagca cggtttcgcc aatagcagcg gtgaccggct ggccgttgat gtggacagtc
     7921 atatccgcct gggtcagggg ctggatatcg aaggttcttt ctaggcagtg cattgcaatg
     7981 ttcatccgtg aaggttcggt tgaggtggtg tcagctcctt gctgcactac accgtgtcga
     8041 cgctgttatt gctttgattg gagacgtcgg cgcggatact tcgtcagcgc aacaagatgc
     8101 gcgagaagca ctgtagttca tacctcagca aaggcctgga tcatttacgt taggcgaagt
     8161 cgcccgacga aatattgatc caggccagtg aaagcggtcg ggttgttcaa cgatctcagt
     8221 tggagaagac gaactgccct ttgatctttt tcgaacccat gaaaccgagg tcgggaaaca
     8281 gcaggttttt cacccacgcc acgaaaaaca ccatcgccag cgtcgtcacc acaccgatga
     8341 tcaccgacac cgggtcttcg gcgtaatcct tgaacagccg tggcatctgg ctgatcgaaa
     8401 gcagcatgta cacggtgtag gtctgcgccg agtggcgatg gaaaccccag gcgaacaaca
     8461 acggcacact accggccagc aacccgagga cgaacgcaag accgtcgctg atcgtcagcc
     8521 ccagtaaata accgcccacc agcccgatca atgcctgcac gtagatcagc ccgagcaata
     8581 ccttgatctt gccggcatgc cgctcaagca attgcggatc cgggcgcgag ccgatccagt
     8641 aggccaggac cttgtccttg accgcaccac cggacaacgc cttgaaggtc tcggttttcg
     8701 agcgcccggc ctccagcatc tgcattgcct gacgtttgat ttccttcttg tccaacgtgc
     8761 ttttccttaa gtagagagat ccggggatgg agcgttgcgc tccagccgcc gattctcgcc
     8821 ttgttgaacc ccggaaagca aaaagccgct tgcaccgtga agtgaaagcg gccgaggcat
     8881 gggcctcaat agagcatttg gatcatagag catttggatc agtgtacgaa cagcgcaatc
     8941 aggatgatga tcgggatcgg gacaccgagg aaaaacagca gtagtgagcg catggcgatt
     9001 ctccttattg agcgttaacg aacagtggtc gtagtggcat aggtttggac ttcaacgtac
     9061 tcgaccgcat cccggcgacg accgccgaaa gtagcggcga ggctggcgaa gaacgcaccg
     9121 gccagcagcg cgatgaacat ccacagcgag gtccaggcag cgactttggc ggcggtatca
     9181 gtcgcctgcc gggctttcac tttggcgtcg gcaatggctt tctgagtgcg ggcgtagacc
     9241 tcatcgacac gacgttcggc atcggcctga ctcaggttgg tgcgctgggc gaccagttgc
     9301 gcaaggtagg tacggtcttc agggctgagt tggccgttgg ccaggctctg ggcgaagatc
     9361 cgggtcaccg cgccatgggc agcgtcatcg ctgaccgatg ctggacggtc atcgcggaac
     9421 aggctgtcga cgaagtagcc gtactggtca ctgttggtgt tcgcggcggc agtaccggcc
     9481 gcctgtgtcg cggcagccgc tgcaccacca gcgacactgg caccggcctg cacaccgcca
     9541 ctgatcacac tgctgaccga gcccaccacc agcaccgcag tcaccaacgt ggccacgcac
     9601 caggcgagga agccatgggc ggtatcacgg aaatacactt catcaccgtg catgttggcc
     9661 cacttcaccc gcaggcggcc ggcgatgtat cccccaagac ccgaagcgat gatttgcgtc
     9721 aacgccagcc agacaatcgt ggaaatcccc aggctcttgg cgctgacgcc ctcgccggcc
     9781 caaggtgaaa cggcagagaa tcccaaaccg aagccgagca gcaccaaaat catcgacaat
     9841 gctgcggccg ccgctgcgcc cgcgaaaatc gcgccccagg acacgcctga cacgttgctc
     9901 gactctcctt cgaaggcagg ataaaaccca tctgaggatc tattcattgt tgtagtgctc
     9961 caggcatgaa aaatggtgtt acaactcgtc agcagaggaa ttgcagcgac cgtgccattc
    10021 gacaacacta aataattcgt taaatttcaa caggttaaaa agactgaact tctccactca
    10081 ttgcaatttg caacaatgga ctgaaagcag cgggttttct gcattgacgg aaaatcggca
    10141 gcgctacccg aatttgcgtg ggtgacaggc tatacatgaa agttaattta aagcgcgccg
    10201 gaaaatactt ggcaaaatgc tgccattccg tttgaaccga tcaaggcctg ctctatgact
    10261 cgtattttga ccatcgaaga cgatgccgtg accgcccgtg aaatcgtcgc cgaactcagc
    10321 agccacggcc tggaagtgga ttgggtcgac aatggccgtg agggcctgga ccgcgccgtc
    10381 agcggcaact acgacctgat caccctcgac cgcatgctgc cggagctcga tggcctggcc
    10441 atcgtcacta ccctgcgtac catgggcgtg gccacgccga ttctgatgat cagcgccctc
    10501 tccgatgtcg atgagcgcgt gcgcggcctg cgtgccgggg gtgatgacta cctgaccaag
    10561 ccgttcgcca ccgacgagat ggccgcgcgc gtggaagtgc tgctgcgccg ccagaacagc
    10621 gtcgccactc aggccaccac gttgcaggtg gccgacctcg aactggattt gatcagccac
    10681 gaagcacgcc gcgccgagca ggtgctgacg ctgctgccga ccgaatacaa gctgctggaa
    10741 tttctcatgc gcaacagtgg gcagatcctg tcgcggatga tgattttcga agaggtctgg
    10801 ggttatcact tcgacccggg caccaacctg atcgacgtac acatcggtcg tctgcgcaag
    10861 aagatcgacg ccgcgggcca tgttccgctg attcgcacgg tacggggctc gggttatgtc
    10921 attgccgaac ccgtctgacg gctggcgctc ttccagcagt cgtctgctgg cgctgtacag
    10981 ttcgctgttc gtggcgtgga gcgcgatcct catgggggtc atgtatttcg aggtctccag
    11041 ttatctcgac aacctcgcca agcattcgct gatgcaacgt cagcatctgt tttcgcattt
    11101 tcgcggtgag caactggaag aagccctcgc cgccagcatg accttcgaca tccgcggcat
    11161 cgacgcctat ggcctgttcg acgcccgggg cgtgtacctc ggtggcgcct tgcagcaact
    11221 gcccgaaggc ctgccgctgg acggcaagat ccacacgctg gatgactgcg ccgactccga
    11281 cgacccgacc ctgcccgccg acagctgtga cgccgtggcg acccagaccc gcgacggtcg
    11341 ctggctggtg ttgttgcgcg acaacggctc gctgttcgcg gtgacgcgga tcattctgca
    11401 tgcgttgttc tggggcgtgt cgctgacgat tctgccgggg attgccggtt ggcatttgct
    11461 gcgtcgccga ccgatgcgcc gcatccgcgc gattcaggac agcgcccagg cgattgtcgc
    11521 cggcgacctg acccaccgct tgccgctgtc caaccgccgc gatgaactgg acatgctggc
    11581 cgccatcgtc aacgccatgc tcgaccgcat cgaacggctg atgcatgaag tcaaaggcgt
    11641 gtgcgacaac atcgcccacg acctgcgcac gccgctgacg cgtttgcgcg cgcagttgta
    11701 tcgcatgcag cagcaggccg gcgaaggttc gcaggaagcg gcgcaactgg acctggtgct
    11761 ggccgaagcg gatacgctga tggcgcggtt tcgcgggttg ctgcggattt ccgagctgga
    11821 agaccgccag cgccgctcag ggtttgtcga actcgatcca gtgaaattgc tcgaagaact
    11881 gcatgagttt tacctgccgc tggccgagga aggcgaactg ctcttcgtgc tgaacatgcc
    11941 ggaaaccctg ccacccttga atggtgaccg ggcgctgctg ttcgaggcgc tggcgaatct
    12001 gttgagcaac tcgatcaagt tcaccccggc gggcgggacg gtgatcctgc gcggtgtgaa
    12061 cgacggtggg cacacgcgga tcgaggttca cgattccggg ccggggattc ccgagaccga
    12121 gcgcgaggcg gtgttccagc gtttctatcg ggccgagggc gggcagccgc aaagcggatt
    12181 tggcctgggc ttgtcgattg tcgcggcgat tgtcagcctg catggcttca atcttgaggt
    12241 gggcagcagt gagctggggg gcgcgaaact ggtgctggat tgtcggcaac gtttgatcgc
    12301 caattcctga tatgacatgg atccccctgt ggaaacgggc ttgctcgcga atgcggtgtg
    12361 tcaggcggcg aagaggctga ttgatcgaac gcttcgcgag caagctcgct cccacattta
    12421 ggtccttgtc aaatcaggtt gggtagttgg cccgcaacgc ttcaagccct ccctgataga
    12481 tgccactgaa caacgccacc acttcctcat cgctcacacc tttggcgcta aagcggcccg
    12541 accacgtcac ccgggcgccc tgcccgtggg cttcgacctt gatcgtcgcc aggtaatcgg
    12601 tggccggaaa cggcgcctgt tcgatcgaat agctataagt cctggcagcg ttgtcgaacg
    12661 cttgcagacg ctcgatcacc acaccgccat cggcggtttg cagggtgcgc acgcgcccgc
    12721 cttcgctcag ctcactcttg gcaatcaacg gcagccagtc cggcagcgtg ttgaagccgc
    12781 cgatcaattg ccagacctga tcggccgagg ccgggatgtc gatcgttgct gatgcagttg
    12841 ccataaatta acgtttctcc agaagaattc gggttcagat cgccatgctg tcgacaacgc
    12901 caccatcgac ccgcagggct gcgccggtgg tggccgagga cagcggcgaa gcgatgtacg
    12961 tgaccagatg cgc
//
