LOCUS       NZ_JAHHZH010000002     10846 bp    DNA     linear   CON 04-JUL-2024
DEFINITION  Pseudomonas sp. MG-9 NODE_2_length_790042_cov_67.6076, whole genome
            shotgun sequence.
ACCESSION   NZ_JAHHZH010000002
VERSION     NZ_JAHHZH010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Pseudomonas sp. MG-9
  ORGANISM  Pseudomonas sp. MG-9
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 790042)
  AUTHORS   Lutfullin,M.T., Pudova,D.S., Lutfullina,G.F., Vologin,S.G.,
            Gogoleva,N.E., Shagimardanova,E.I., Sharipova,M.R. and
            Mardanova,A.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) Microbiology, Kazan Federal University, 18
            Kremlyovskaya, Kazan, Tatarstan Republic 420008, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2021
            Assembly Method                   :: SPAdes v. v. 3.12
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018729515.1-RS_2024_07_04
            Annotation Date                   :: 07/04/2024 03:27:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,536
            CDSs (with protein)               :: 5,536
            Genes (RNA)                       :: 89
            rRNAs                             :: 8, 9, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 6, 8 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 25 of 68
            Pseudo Genes (incomplete)         :: 48 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:31:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 166635
            Orig. end                         :: 177481
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHHZH010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10846
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10846
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10846
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5846](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5846
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    11..26
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.42"
                     /tool="antismash"
     gene            573..1613
                     /locus_tag="KKQ10_RS05435"
                     /old_locus_tag="KKQ10_05435"
     CDS             573..1613
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016984038.1"
                     /locus_tag="KKQ10_RS05435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_05435"
                     /product="protease modulator HflC"
                     /protein_id="WP_127649544.1"
                     /transl_table=11
                     /translation="MSQSHTHDSHDHSGHDHGHAGHHHHHGHHHHGAPEEAGPFPWRRM
                     GWAVLLVAFAVAAASLVQVRSGEATVITRFGNPSRVLLEPGLSWRWPAPFEAAIPVDLR
                     LRTTSSGLQDVGTRDGLRIIVQAYVAWQVQGDPDNVQRFMRAVQNQPDEAARQIRTFVG
                     SALETTASSFDLANLVNTDASQVRIADFEAQLRQQIDQQLLATYGVRVVQVGIERLTLP
                     SVTLTATVDRMRAERETIATERTAIGKREAAQIRSAAERDARIVQADATVKAAEIEAQS
                     RVEAAQIYGRAYAGSPQLYNLLRSLDTLGTIVTPDTKLILRTDAAPFRVLVDGPPAVDN
                     KSGSQP"
     gene            1610..2671
                     /locus_tag="KKQ10_RS05440"
                     /old_locus_tag="KKQ10_05440"
     CDS             1610..2671
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912025.1"
                     /locus_tag="KKQ10_RS05440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_05440"
                     /product="protease modulator HflK"
                     /protein_id="WP_215500477.1"
                     /transl_table=11
                     /translation="MSDEVPRGTHSLSSPWIQAGRLAFFALYAVTVLAALAWAFSNVRQ
                     IDPQNRAVVLHFGALDRIQNAGLLLAWPQPFEQVVLLPAADRVIERRVENLLRSSEAIK
                     ADRVATFATPLSDALAGSGYLLTGDAGVVQLDVRVFYKVTDPYDFVLQGEHVLPALDRL
                     VTRSAVALTAARDLDTILVARPELLGADNQAAERRERLRGDLVQGINKRLAELKASGQG
                     IGIEVARVDVQSSLPDPAVSAFNAVLTASQQADKAVANARTEAEKLTQTANEQADRTLQ
                     VAHAQAGERLAKASTDTATVMSLAKAQQQGTDPQMLLRLYRERMPKVLGQAGSVTTVDP
                     KDDSRLIIQGASK"
     gene            2668..4575
                     /locus_tag="KKQ10_RS05445"
                     /old_locus_tag="KKQ10_05445"
     CDS             2668..4575
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008007844.1"
                     /locus_tag="KKQ10_RS05445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_05445"
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="WP_215500478.1"
                     /transl_table=11
                     /translation="MTATTAAPSLLSSAEQRRAARQLTLAMLALGLLGLGLIWRWLVPE
                     QTGVSQLLLGFASLLVAVPVMRSAWYSLRYPSLHGITDQLIALAMLGAWATGDLLTAAL
                     LPIIMIFGHVLEERSVIGSQEAIHALGQLTRSHARKIQADGSIIEVDNGTLKPGDTVEV
                     RAGDRVPADGRVLFGQASLDTASITGESVPVETAVGMTVFGGAINLDGLLRIEVTRTGD
                     QSTLGKVIALMQNAERSKPPITRLLERYAGSYMVLVLLLAAVTWFVTNDAQAMLAVLVA
                     ACPCALVLSAPATAIAGVAVAARHGILIRSSAFLEELADLTSLVVDKTGTLTYGTLRLQ
                     SISSPRLDQASVMALAASLGAASSHPVSRALAGLVSQEQSLPLSDIHERQGLGVVAMTT
                     QGEAALGRPELFAQLGIVTTTVPEHDGPIAGLALNGEFVAWLLLADSVKPEARFALAEL
                     RELGLGRQLLLTGDRQSVAQTLAREVGLQEVEAQALPEDKLNRVLKEIDNGFRPMVVGD
                     GINDSLALKAGVVGVAMGAGGADIALASADIVLIGSDLRRLGTCVRLSRQCRRTLQVNV
                     IIGLGWTLAIVVFAAFGWLGAAGAMIAALLHNLSTLLVLGNAGRLLRFQEPLLKLQDDR
                     "
     misc_feature    complement(4680..4694)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.87"
                     /tool="antismash"
     gene            4685..4936
                     /locus_tag="KKQ10_RS05450"
                     /old_locus_tag="KKQ10_05450"
     CDS             4685..4936
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912027.1"
                     /locus_tag="KKQ10_RS05450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_05450"
                     /product="membrane protein"
                     /protein_id="WP_025112611.1"
                     /transl_table=11
                     /translation="MTIKTAAAGAALALAAATMFAGVATNAMAADAQVHCYGVNACKGQ
                     NDCKTKDHACKGMGSCKGQGFKAMTQSACEKAGGKVGE"
     gene            5001..5846
                     /locus_tag="KKQ10_RS05455"
                     /old_locus_tag="KKQ10_05455"
     CDS             5001..5846
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927346.1"
                     /locus_tag="KKQ10_RS05455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_05455"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_215500479.1"
                     /sec_met_domain="DUF692 (E-value: 2.7e-101, bitscore:
                     333.8, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSASVPFLGYGLGLRSAYYQQILEQSPDVDWFEVVSENFMVQGGK
                     ALYFLDAIAERYPVVMHGVSLSIGGPHALDPDYLKQLKQLAERVKPAWISDHLCWSRGN
                     AHQLHDLLPLPYTEESLEYIAARVAQVQDVLQRPLVLENVSSYVRVAADDFTEWEFLQA
                     LSHVSGCELLLDVNNVYVSSRNHGFDPWTFIQSLPKDKVRQLHLAGHSDYGDYVIDTHD
                     HPVSDPVWALYQRTLEHFGPVATLLERDDHFPPFEELLDELHKARELGDRVLAGRQKCA
                     "
     gene            5837..6622
                     /locus_tag="KKQ10_RS05460"
                     /old_locus_tag="KKQ10_05460"
     CDS             5837..6622
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016984033.1"
                     /locus_tag="KKQ10_RS05460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_05460"
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_215500480.1"
                     /transl_table=11
                     /translation="MRLNEWQLAFEAFLLEESTAAPPALSSSLIGGPTLGVDTGLAIYH
                     NAYKARLLEVLRNDFPVIYHWLGDEAFDALAGAYLRQHPSTHYSLRWLGQGFEAFIREY
                     LVPEQSAPLSELATLEWAFTLAFDAPPGVPLTIETMAGFSPQEWPALQVKPRPSLQWLE
                     QRFNSLALWRSVKDATDFPDSEALEAPQVCLIWRSNLLCNYRSLESAEANALNGMINQG
                     WSFAELCAELAVIYGEGAPLRAVTWLKQWVHDGMLERLE"
     gene            6710..7897
                     /gene="cfaB"
                     /locus_tag="KKQ10_RS05465"
                     /old_locus_tag="KKQ10_05465"
     CDS             6710..7897
                     /NRPS_PKS="Domain: oMT (132-232). E-value: 4e-19. Score:
                     59.9. Matches aSDomain: nrpspksdomains_KKQ10_RS05465_oMT.1"
                     /codon_start=1
                     /gene="cfaB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016984032.1"
                     /locus_tag="KKQ10_RS05465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_05465"
                     /product="C17 cyclopropane fatty acid synthase CfaB"
                     /protein_id="WP_215500481.1"
                     /transl_table=11
                     /translation="MLAQLPPALQNLQLPLRLRLWDGHEFNLGPAPSVTIVVKDPTMVS
                     QFTHPSLDALGAAFVEGKLELEGSISEVIRVCDELSSALLDEDDDAQPVRAVHDKETDA
                     KAISYHYDLSNAFYQLWLDSDMAYSCAYFETGSETLEQAQQAKFRHLCRKLRLQPGEYL
                     LDVGCGWGGLARYAAREFGAKVFGITLSKEQLELARERVKAEGLEDQVELQLLDYRDLP
                     QDGRFDKVVSVGMFEHVGHANLAEYCKVLFGAVKEGGLVMNHGITAKHTDGRPVGRGAG
                     DFIEKYVFPNGELPHLAMISAQISEAGLEIVDVESLRLHYARTLDHWSERLEDNLEAAG
                     KLVPDQALRIWRLYLAGCAYAFARGWINLHQILAVKAHPDGSHELPWTRDDIYHP"
     aSDomain        7106..7405
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KKQ10_RS05465_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="4.00E-19"
                     /label="KKQ10_RS05465_oMT.1"
                     /locus_tag="KKQ10_RS05465"
                     /protein_end="232"
                     /protein_start="132"
                     /score="59.9"
                     /tool="antismash"
                     /translation="FETGSETLEQAQQAKFRHLCRKLRLQPGEYLLDVGCGWGGLARYA
                     AREFGAKVFGITLSKEQLELARERVKAEGLEDQVELQLLDYRDLPQDGRFDKVVS"
     gene            complement(7935..9374)
                     /gene="cls"
                     /locus_tag="KKQ10_RS05470"
                     /old_locus_tag="KKQ10_05470"
     CDS             complement(7935..9374)
                     /GO_function="GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /GO_process="GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="cls"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016984031.1"
                     /locus_tag="KKQ10_RS05470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_05470"
                     /product="cardiolipin synthase"
                     /protein_id="WP_215500482.1"
                     /transl_table=11
                     /translation="MDYFGPHIFGYLIALIHSLGLIAAIHAVLTVRTAQGSIAWALSLI
                     FIPYLTLIPYLVFGRSTFDGYIKARRQANEQMRQAISELNWRPWVEEALTARASNAYAS
                     LRAMPKLGRMPCLANNEVRLLVNGHATFSAIFQAIEQAREAVLIQFFIIHDDRLGQSLR
                     DLLLKKAAEGVAIHLLYDRIGSHALPHSYVQALRDGGVEVKAFATRSGWLNRFQVNFRN
                     HRKIVVVDGVLGFVGGHNVGDEYLGEKPPLAPWRDTHVQVRGPVVACMQESFAEDWFWA
                     ARTLPPLILPDVYPEDGVLCQLLASGPADAYETCSLFFVEAIHAATERVWITSPYFIPD
                     EAVFAALRLAVLRGVDVRLLLPSRPDHRIVYAASSLYAFEAVRAGVRVFRYEPGFMHQK
                     VVLIDSEISAIGSANLDNRSFRLNFEVMLLTVDSEFAANVEHMLNDDFEQAYEVAKEES
                     REIHRLQQLGMRIARLISPIL"
     gene            9507..10025
                     /locus_tag="KKQ10_RS05475"
                     /old_locus_tag="KKQ10_05475"
     CDS             9507..10025
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341609.1"
                     /locus_tag="KKQ10_RS05475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KKQ10_05475"
                     /product="DUF3617 domain-containing protein"
                     /protein_id="WP_215500483.1"
                     /transl_table=11
                     /translation="MNVRLLGLALGLGLAMPVIAQAQMLQPGLWEMTSSNVKVDDQPMD
                     VQSILGQLQGQMTPQQRAALEKNGINIGGQGIRACLTPQQVATNDIPLADPQSGCKQQI
                     TERTGNQWKFRFSCPKAQGTGVATFKSDREFTTVANGTFNAIGINQKGSLETRAVWLGQ
                     DCGAVKPRA"
     misc_feature    10092..10103
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 16.98"
                     /tool="antismash"
     misc_feature    complement(10218..10233)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.55"
                     /tool="antismash"
ORIGIN
        1 cggattctgg ttcacgattt tgcctcgcgc accctcgacg gcctgctggg ggcgtatcgg
       61 gtaggactgg gcgaagaaat cggccgcgcg gtgcaagacg atctgcagaa actcgacagc
      121 ggcgtggaga ttctcgccac cgtggtcgag gcgattcatc cacctgccgg tgccgccaat
      181 gcttatcacg gcgtgcaagc ggcgcagatc ggtgcacagg cgttgatctc ccgggagcgc
      241 ggtgctgcgg ctgaagccag taaccaggcg cagttgcagg ccagccttgt ccgcgaccag
      301 gccagcgcca gtgcgcgtga ggtcaacgcc actgcacaag cggcggatct gaagttcaag
      361 gctgaacaac aagcttatgc cagcgccggc caggcattcg tgctggagca atatttcagt
      421 cagctcagcc agggcctgag caaggccaaa ctgctggtgc tcgaccaccg tttaggcggc
      481 agcagcaatg cgccgaccat cgacctgcgt acgttcacgc tgccggctga cccgacgccc
      541 gcgcgcacca ccgctcaacc aggagttgcc cattgagcca gtcgcacact cacgattcgc
      601 atgatcacag cggccacgat cacggccacg ccgggcatca ccatcatcat gggcatcatc
      661 accacggtgc gccggaagag gcgggcccgt tcccgtggcg gcgcatgggc tgggcggtgt
      721 tgctggtggc gtttgccgtc gcggcggcga gcctggtgca agtgcgctcg ggggaggcca
      781 cggtcatcac ccgtttcggc aatccgtcgc gggtgttgct tgaacccggt ttgagctggc
      841 gctggccggc accgttcgaa gcggcgatcc cggtggattt gcgcctgcgc accacgtcca
      901 gcggtttgca ggatgtcggc acccgcgacg gtttgcgcat catcgttcag gcctacgtgg
      961 cgtggcaggt ccagggcgac ccggacaacg tgcagcgttt catgcgtgcg gtgcagaacc
     1021 agccggatga agcggcgcgg cagattcgga cctttgtcgg ctcggcactg gaaaccaccg
     1081 ccagcagttt cgacctggcg aatctggtga acaccgatgc gagtcaggtg cgcatcgccg
     1141 atttcgaagc gcaattgcgt cagcagatcg atcagcaact gctcgccact tacggcgtgc
     1201 gcgtggtaca ggtcggcatc gaacggctga ccttgccctc ggtgacgctc acggcgacgg
     1261 tcgatcggat gcgtgccgag cgtgaaacaa tcgccaccga acgcacggcc attggcaagc
     1321 gtgaagcggc gcagatccgt tcggccgccg agcgtgatgc gcggattgtg caagccgatg
     1381 ccacggtgaa agctgcagaa atcgaagcgc agtcccgggt cgaagccgct cagatctacg
     1441 gccgtgccta cgccggttcg ccgcagctgt acaacctgct gcgctcactg gataccctcg
     1501 gcaccatcgt tacgccggac accaagctga tcctgcgcac cgatgccgcg ccattccgcg
     1561 tgctggtgga cggtccgccc gctgtcgata acaaatccgg atcgcaacca tgagtgatga
     1621 agttccacgt ggaacacatt cgttgagcag tccgtggatt caggccggac gcctggcgtt
     1681 ttttgccttg tacgcggtga ccgttttggc tgcgttggcg tgggcgtttt ccaatgtccg
     1741 gcagatcgat ccgcagaatc gcgctgtggt tttgcacttt ggtgcactgg atcgcatcca
     1801 gaatgccggc ctgttgctgg cctggccgca accgttcgaa caagtggttt tactgcccgc
     1861 agcagatcga gtgattgaac gccgagtgga aaacctgctg cgcagcagtg aggcgatcaa
     1921 ggctgaccgc gttgcgacct tcgccacacc gctgagcgat gccttggccg gttccggcta
     1981 cttgctgacc ggcgacgccg gtgtggtgca actggatgta cgggtgtttt acaaggtcac
     2041 cgatccgtat gactttgtgt tgcagggtga gcatgtgctg ccagccctgg atcggctggt
     2101 cacccgcagc gctgtagctt tgaccgccgc ccgggatctg gacacgattc tggtcgcccg
     2161 cccggagttg ctcggcgctg acaatcaggc ggccgaacgc cgcgagcgtc tgcgcggtga
     2221 tctggtgcaa ggcatcaaca aacgcttggc agagctgaag gcgagcgggc agggcatcgg
     2281 catcgaagtg gcgcgggtcg acgttcagtc gagtctgccg gacccggccg tcagcgcctt
     2341 caacgctgta ttgactgcca gccagcaagc cgacaaagcg gtcgctaacg cgcgcaccga
     2401 ggcggaaaaa ctcactcaga ccgccaacga acaagccgac cgcaccctgc aagtggccca
     2461 cgcgcaggcc ggcgaacggc tggcgaaagc ctccaccgac accgccacgg tcatgagcct
     2521 ggccaaggcg cagcaacagg gcaccgatcc gcagatgctc ctgcgtctgt accgcgaacg
     2581 catgccgaaa gttcttggcc aggccggttc cgtgaccacg gtcgatccga aagacgattc
     2641 ccgcctgatc attcagggag ccagtaaatg actgcgacca ctgccgcacc gagcctgttg
     2701 tcctcggccg aacagcgccg cgctgcacga caattgaccc tggcgatgct cgcccttggc
     2761 ttgctcggcc tcggtctgat ctggcgttgg ctggtgccgg agcaaaccgg tgtcagtcag
     2821 ttattgctgg gttttgcttc tttattggtg gcggtgccgg tgatgcgttc ggcgtggtac
     2881 agcctgcgtt acccgagcct gcacggcatc accgatcaac tgattgcgct ggcgatgctc
     2941 ggtgcgtggg cgaccggtga tttgctgacc gccgcgctgc tgccgatcat catgattttc
     3001 ggtcacgtgc tggaagagcg cagcgtgatc ggttcgcagg aagcgattca cgccctcggt
     3061 caattgaccc gcagccacgc acgcaagatt caggcagacg gctcaatcat cgaagtggat
     3121 aacggcacgc tcaaaccggg cgacaccgta gaggtgcgcg ccggtgatcg ggtcccggcg
     3181 gatggtcggg tgctgttcgg ccaggccagc ctcgacacag catcgatcac tggcgagtcg
     3241 gttccggtcg aaacggcggt gggcatgacg gtgttcggcg gggcgatcaa cctcgatggc
     3301 ttgctgcgca ttgaagtgac ccgcaccggc gaccaatcga cactgggcaa agttatcgcg
     3361 ctgatgcaaa acgccgagcg ctcgaagccg ccgatcaccc gtctgcttga gcggtatgcc
     3421 ggcagctaca tggtgctggt gttgttgctg gcggcggtga cctggtttgt caccaacgac
     3481 gcgcaagcga tgctcgcagt gttggtcgca gcctgtccct gcgctttggt gctgtcggcg
     3541 ccggctacgg cgattgccgg ggtggcggta gcggcgcgcc acggcattct gatccgcagc
     3601 tcggccttcc tcgaagagct ggcggacctg acctcgctgg tggtcgacaa gaccggcacc
     3661 ctgacttacg gcactttgcg tttgcaatcg atcagcagcc cgcgtctgga tcaggcttcg
     3721 gtgatggcac tggccgccag tctcggcgcg gccagcagtc acccggtcag ccgtgcgttg
     3781 gccgggctgg tcagtcagga gcagagcctg ccactgtccg atattcatga gcgccagggc
     3841 ctcggcgtgg tcgccatgac cacccaaggc gaggcggcgt tggggcggcc tgagttgttc
     3901 gcgcaactgg gcatcgtcac caccaccgtt cccgagcatg acgggccgat tgccgggttg
     3961 gcgttgaacg gcgagtttgt tgcctggctg ttgctggctg acagcgtcaa accggaagcg
     4021 cggtttgctc tcgccgaatt gcgcgaactc ggcctgggtc gccagttgtt gctgaccggt
     4081 gaccgccaga gtgtcgctca gaccttggcc cgggaggttg gcctgcaaga agtggaagcg
     4141 caggcgctgc ctgaggacaa actcaatcgc gtgctcaagg aaatcgacaa cggtttccgg
     4201 ccgatggtgg tgggcgacgg catcaatgat tcgctggcac tcaaggccgg ggtggtcggt
     4261 gtcgcgatgg gcgccggcgg tgctgacatc gcgctggcct cggcggacat cgtgctgatc
     4321 ggcagcgacc tgcgccggct cggcacctgt gtacggctga gtcgccaatg ccgccggacc
     4381 ttgcaggtca acgtcatcat tggtctgggc tggacgctgg cgatcgtggt gttcgcggcc
     4441 ttcggctggc tcggagcggc gggggcgatg attgccgcgt tgctgcataa cctcagcacg
     4501 ctgctggtgc tgggcaatgc cgggcgtttg ttgcggtttc aggaaccgct gctcaagctt
     4561 caggacgatc gttaaattta tttctgcacc tctgtaacgc cgtcggggca gccctctcta
     4621 gtgctatgtc gggactgaac aaccttccag ccgacacccc ctgacgctta ccgaggatat
     4681 aaaaatgacg atcaagaccg ctgctgccgg tgccgctctc gctttggccg ctgccacgat
     4741 gtttgccggt gtcgccacca acgccatggc ggctgatgcc caggttcact gctacggcgt
     4801 gaacgcctgc aaaggccaga acgactgcaa gaccaaggac cacgcgtgca aaggcatggg
     4861 ttcctgcaag ggccagggct tcaaggccat gactcagtcg gcttgtgaaa aagccggtgg
     4921 caaggtcggc gagtaagcca cctgtgagtg ctgccgcagc gggtgatgcc gctgcggcca
     4981 ctttgcaaag gagtcctggc atgtcagctt ccgttccttt cctgggttat ggcctgggtt
     5041 tgcgcagcgc ctattaccaa cagatcctcg aacagtcgcc ggacgtggac tggttcgaag
     5101 tggtttccga aaacttcatg gtgcagggcg gcaaagcctt gtacttcctc gacgccatcg
     5161 ccgaacgcta ccccgtggtg atgcatggcg tgtccctgtc catcggtggg ccgcatgcgc
     5221 tcgatccgga ctatctcaaa caactcaagc aactggccga acgggtcaaa ccggcgtgga
     5281 tctccgatca cctgtgctgg agccgcggca atgcccatca gttgcatgac ctgctgccgc
     5341 tgccctacac cgaagaaagc cttgaataca tcgccgcccg agtggcacag gtgcaggacg
     5401 tgttgcagcg gccattggtg ctggagaacg tttccagtta cgtgcgcgta gccgccgatg
     5461 atttcactga gtgggaattt ctgcaggccc ttagtcacgt cagcggctgc gaattgctgc
     5521 tggacgtcaa caacgtctac gtcagctcac gtaatcatgg cttcgatccg tggacgttca
     5581 ttcagagttt gcccaaggac aaggtgcgtc agctgcatct ggccgggcat agcgattacg
     5641 gcgactacgt gatcgacacc cacgatcatc cggtgagcga tccggtctgg gcgctgtatc
     5701 agcgcacgct ggagcacttc gggccggtgg cgacgctgct ggagcgcgac gatcacttcc
     5761 cgccgttcga agagctgctc gacgaactgc acaaggcccg cgaactgggt gacagggttt
     5821 tggccgggag acagaaatgc gcctgaacga atggcaattg gcatttgagg cctttctgct
     5881 ggaggagtcg acggcagcac cgccggcctt gagcagcagc ctgatcggcg gcccgacgct
     5941 gggtgtcgac accgggctgg cgatctatca caacgcctac aaggcgcgtt tgctcgaggt
     6001 gctgcgtaac gacttcccgg tgatctatca ctggctgggg gatgaagcgt tcgacgcgct
     6061 tgcgggtgcg tacctccgtc agcatccgtc gacgcattac agcctgcgtt ggctcggcca
     6121 gggtttcgag gcgtttatcc gcgagtatct ggtgccggag caaagtgcac cgttgagtga
     6181 actggcgacg ctggagtggg cgttcactct ggcttttgat gcgccgcccg gcgtgccgtt
     6241 gacgatcgag acgatggcgg ggttttcccc acaggaatgg cccgctcttc aggtcaaacc
     6301 gaggccctcg ctgcagtggc ttgagcagcg tttcaacagt ctggccttgt ggcgttcagt
     6361 caaggatgca acggatttcc cggacagcga ggcacttgaa gccccgcagg tgtgcctgat
     6421 ctggcgcagt aatctgctgt gcaattaccg cagtctggag tcggccgagg ccaatgccct
     6481 gaatggcatg atcaatcagg gctggagttt tgccgaactg tgcgccgagt tagcagtcat
     6541 ctatggtgag ggcgcgccac ttcgagccgt tacctggtta aagcagtggg tgcatgacgg
     6601 catgctggag cgtctggaat gatagtctcg gctaattaaa tcgatagcct gctatttttc
     6661 aaggatggtc taccctcaga tctgacgtct gaaacaaggg ggattttcca tgctcgcgca
     6721 acttccaccg gccttacaga atctgcagct tccgcttcgc ttgcgactct gggatggcca
     6781 tgagtttaat ctggggccgg cgcccagcgt caccatcgtg gtcaaggacc cgacgatggt
     6841 ctcccagttc acgcatccaa gtctcgacgc gctgggggcg gcgtttgtcg aaggcaagct
     6901 tgaactggag ggctcgatca gcgaggtcat ccgggtctgc gacgaattga gcagcgcctt
     6961 gctcgatgaa gacgatgatg cccagccagt gcgtgcggtg cacgacaagg aaaccgacgc
     7021 caaagccatt tcctatcact acgacctgtc caacgcgttc taccagctgt ggctggacag
     7081 cgacatggcg tattcctgcg cgtatttcga aaccggcagc gaaaccctgg aacaggcgca
     7141 acaagccaaa ttccgccatc tgtgtcgcaa gctgcgcctg caacccggcg aatatctgtt
     7201 ggatgtcggc tgtggctggg gtggtctggc gcgatatgcc gcgcgggagt tcggtgccaa
     7261 ggtgttcggc atcaccctga gtaaagaaca gctggagttg gctcgcgagc gggtcaaggc
     7321 tgaagggctg gaagatcagg tcgaactgca actgctcgac taccgtgatc tgccgcagga
     7381 cggtcgtttc gacaaggtgg tcagcgtcgg catgttcgaa cacgtcggcc acgccaacct
     7441 cgccgaatac tgcaaagtcc tgttcggcgc agtgaaggag ggtggtctgg tgatgaacca
     7501 cggcatcacc gccaaacaca ccgatggccg tccggtggga cgcggtgccg gcgacttcat
     7561 cgagaaatac gtgttcccca acggcgagct gccacacctg gcgatgatct cggcacagat
     7621 cagcgaagca gggctggaga tcgtcgatgt cgaaagtctg cgcctgcatt acgcacgtac
     7681 gctcgaccac tggagcgagc gcctggagga taaccttgaa gctgccggaa aactggtacc
     7741 ggatcaggca ttgcgcatct ggcggctgta tctggccggg tgtgcctacg cgtttgcgcg
     7801 tggctggatc aatctgcacc agattctggc ggtgaaggcg catccggatg gcagccatga
     7861 actgccctgg actcgcgacg atatctatca cccttgaggc ttgaaccctc tctcggttgt
     7921 ggggggggaa acgtctagag aatcggtgaa ataagccggg cgatccgcat tccgagctgt
     7981 tgcaggcggt ggatctcccg gctttcttct ttggcgactt cgtaagcctg ctcgaaatcg
     8041 tcgttgagca tgtgttccac gttagcggcg aactcgctgt cgaccgtcaa cagcatcact
     8101 tcgaaattca gccggaacga acggttgtcc agattggcgc tgccgatggc gctgatttcg
     8161 ctgtcgatca acaccacttt ctgatgcatg aacccgggtt cgtagcggaa cacccgcacg
     8221 ccggcgcgca ccgcttcgaa cgcatagagg ctggacgcgg cgtaaacaat gcggtgatcg
     8281 gggcgcgagg gcagcagaag gcgcacgtcg acgccgcgca gcaccgccag gcgcaacgcg
     8341 gcgaataccg cctcatcagg gatgaagtag gggctggtga tccacacccg ctccgtggcg
     8401 gcgtgaatcg cttcgacgaa gaacaatgaa caggtttcgt aggcatcggc cgggccactc
     8461 gccagcaatt ggcagagcac gccatcttcc gggtagacat ccggcagaat cagcggcggc
     8521 agcgtgcgcg cggcccagaa ccagtcttcg gcaaacgatt cctgcatgca ggccactacc
     8581 gggccgcgca cttgaacgtg ggtatcgcgc catggcgcca gtggcggttt ttcaccgagg
     8641 tattcatccc ccacgttgtg cccgccgaca aagccgagca cgccatcgac cacgacaatt
     8701 ttgcggtggt tgcggaagtt cacctgaaag cgattgagcc agccgctgcg cgtggcgaag
     8761 gctttgacct cgacaccgcc atcgcgcaat gcctgcacat agctgtgggg cagggcgtgg
     8821 ctgccgatcc ggtcgtagag cagatgaatc gccacgcctt cggcggcttt cttcagcagc
     8881 aggtcgcgca ggctttgacc gagacggtca tcatgaataa tgaagaactg aatcagcaca
     8941 gcttcgcgcg cctgttcgat ggcctggaaa atcgctgaaa acgttgcgtg gccattcacc
     9001 agcagccgta cttcgttgtt cgccagacac ggcatgcgcc ccagcttggg catggcgcgg
     9061 agcgaggcgt aggcatttga ggcgcgggcg gtgagagctt cttctaccca cggtcgccaa
     9121 ttcaattcgg aaatcgcctg gcgcatctgt tcgttggcct ggcgccgtgc cttgatgtag
     9181 ccgtcgaacg tgctgcggcc gaagaccagg taagggatga gggtcaggta ggggatgaaa
     9241 ataagcgaca atgcccaggc gatcgagcct tgggcggtgc gtacggtcag caccgcatgg
     9301 atggcggcga tcaagccgag gctatggatc agggcgatca gataaccgaa aatgtgcggt
     9361 ccaaaataat ccatgaggca gccttgctcc tgaagattca atgtctaaca gaccatgttc
     9421 ccgagcgatt gtcgctattt aattggccca tgaaccgaag ccattggccg acgtctaacg
     9481 gccactactg atcaggagtt ttttcgatga acgttcgtct gctgggtttg gcgctgggcc
     9541 tgggtttggc aatgccggtg attgcacagg cgcaaatgct gcagccgggt ttgtgggaaa
     9601 tgacatcaag caacgtcaag gtcgatgacc agccgatgga tgtgcaatcg atcctgggcc
     9661 agttgcaagg ccagatgacc ccgcaacagc gcgcggcact ggagaagaac gggatcaata
     9721 tcggcggcca gggcatccgc gcctgtctga cgccgcagca ggtcgcgacc aacgatatcc
     9781 cgctggccga tccacaatcg ggctgcaaac agcagatcac cgagcgcacc ggcaaccagt
     9841 ggaaattccg cttcagttgc ccgaaagcgc aaggcaccgg tgtggccacg ttcaagagtg
     9901 atcgtgagtt caccacggtc gccaacggca cgttcaatgc catcgggatc aaccagaagg
     9961 gcagcctgga aacccgcgcg gtgtggctgg ggcaggattg cggcgctgtg aagccaagag
    10021 cttaaaagct tcgcgagcaa gcccgttccc acagggaaat gcattccaac tgtgggagcg
    10081 agcctgctcg cgaaagcgtt ttgatgatca ccgcataaac cgcagcggca gcccctgaca
    10141 cccctcattc acccgcccgc catcccaggc gatctgccct gacaccagtg ttgtatttac
    10201 ccgatatcgg aaagtcctgt tggcaaatgg cgtccatccg cactgcgaca gaatcggttg
    10261 tcgagacacc tccaaagctg ttggctccac caacaccaga tccgcccaat acccttcccg
    10321 caaataacct cggtccggaa tcgcgaacag atctgccacg cggtggctgg tttttgccac
    10381 cagtgtggtg atcggcagca cgccgtcggc caccagttcc agcagcgcgg gcaacgcctg
    10441 ttgcaccagt ggcagaccgg aaggcgcttc ggcatacggg cgctgctttt ccgcccaggt
    10501 atgcggtgcg tgatcgctgc cgatcacatc cagtcggtga ctcaacaagg cctgacggag
    10561 cgcatctcgg tcgacctggg tcttgatcgc cgggttgcat ttgatcaggt tgccgagagc
    10621 cggatagtcg cgatcatcga acagcaggtg atgcaggcag acttcagccg tgatgtgttt
    10681 ctgtgcgagt ggtttgtcct cgaacaacgc caactcgcga gcggtggtca ggtgcaagac
    10741 atgcaggcgg gtgccgtgcc gtctggccag ttccactgcc agcgaagagg agcggtagca
    10801 agcctcggca tcgcggatca gtgcgtggac attcgccggc aggcgt
//
