LOCUS       NZ_JAHFZZ010000065      2432 bp    DNA     linear   CON 13-SEP-2024
DEFINITION  Nonomuraea sp. NEAU-A123 Scaffold65, whole genome shotgun sequence.
ACCESSION   NZ_JAHFZZ010000065
VERSION     NZ_JAHFZZ010000065.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nonomuraea sp. NEAU-A123
  ORGANISM  Nonomuraea sp. NEAU-A123
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Nonomuraea.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Zhang,L. and Zhao,J.
  TITLE     Nonomuraea triticisoli sp. nov., a novel actinobacterium isolated
            from rhizospheric soil of wheat in Langfang, Hebei Province, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Zhang,L. and Zhao,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2021) College of Life Sciences, Northeast
            Agricultural University, Changjiang Road No. 600, Xiangfang
            District, Harbin, Heilongjiang Province 150036, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018603905.1-RS_2024_09_13
            Annotation Date                   :: 09/13/2024 04:26:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11,983
            CDSs (total)                      :: 11,901
            Genes (coding)                    :: 11,761
            CDSs (with protein)               :: 11,761
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 28 of 140
            Pseudo Genes (incomplete)         :: 121 of 140
            Pseudo Genes (internal stop)      :: 3 of 140
            Pseudo Genes (multiple problems)  :: 12 of 140
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:52:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2432
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHFZZ010000065.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     aSDomain        complement(<1..605)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59310_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.00E-73"
                     /label="KLK06_RS59310_Condensation_LCL.1"
                     /locus_tag="KLK06_RS59310"
                     /protein_end="811"
                     /protein_start="609"
                     /score="239.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIASDG"
                     /tool="antismash"
                     /translation="ERVPLSFAQRRLWFIRQLEGPSATYNVPIVVRLDGEVDVEALGAA
                     LRDVIERHEVLRTVFPVADGQPYQRILEPDELIWRMERVSSADLEVAEAAGYAFDLASE
                     VPIRAWLIDERVLVVVIHHIASDGWSRAPLARDVSVAYAARHDGRAPEWEPLPVQYADY
                     ALWQRELLGDDQDPDSVISRQVAYWRETLAGAPEELALP"
     source          1..2432
                     /collection_date="2016-05-10"
                     /db_xref="taxon:2839649"
                     /geo_loc_name="China: Langfang"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Nonomuraea sp. NEAU-A123"
                     /strain="NEAU-A123"
                     /submitter_seqid="Scaffold65"
     gene            complement(<1..>2432)
                     /locus_tag="KLK06_RS59310"
                     /old_locus_tag="KLK06_59315"
     region          1..2432
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2432
                     /SMILES="NC(CC(=O)N)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2432
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:2432](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2432)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             complement(<1..2432)
                     /NRPS_PKS="Domain: AMP-binding (30-423). E-value: 1.1e-95.
                     Score: 312.6. Matches aSDomain:
                     nrpspksdomains_KLK06_RS59310_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (526-595). E-value: 3e-28. Score:
                     89.8. Matches aSDomain: nrpspksdomains_KLK06_RS59310_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (609-811). E-value:
                     2e-73. Score: 239.0. Matches aSDomain:
                     nrpspksdomains_KLK06_RS59310_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /locus_tag="KLK06_RS59310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_59315"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_214644734.1"
                     /sec_met_domain="AMP-binding (E-value: 3.4e-105, bitscore:
                     338.0, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.2e-18, bitscore:
                     53.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.8e-43, bitscore:
                     134.8, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVLDEVERRLVLEGWNATAVEVGGVSVVELFEGQVARRPDAVAVR
                     SGGVAVSYAGVEERANRLAQVLVGEGVGVESVVGVCLGRGVDLVVALLAVWKVGAGYLP
                     IDPGLPVERVAFMLADSGAVLTVTSAEIAEELPAGGGRMLVLDDALTVMRLSMASAERP
                     ERHVVGDQIAYVIYTSGSTGRPKGVVVTQAGLANYVGWAAGFYGVGVGSVLHSSVAFDL
                     TVTGLWVPLVLGAEVVVSEGGGVDGLVGVLSEGLGLGLVKVVPAHLSLLAEVVSSDVGR
                     TWVVGGEVLSGGLVRSWLARSPGSVVVNEYGPTETVVGCCVFEASVEVGESVPIGRPIA
                     NCRLYVLDGWLRPVPPGVAGELYIAGVQVARGYVGRPGLSAERFVACPFELGGRMYRSG
                     DVVRWGVDGDLEFLGRADEQVKIRGFRVEPGEVQAVVAAHPLVAQAAVVAREDVPGDVR
                     LVAYVVGADGVADLVRRFVVERLPEYMVPSVVVALEALPLTSNGKLDRGALPVPEQGVG
                     GGRVPVTVREGLLCQAFAEVLGLSVVGVDDDFFALGGHSLLAVRLVSRVRALLGVELGL
                     SVLFEAPTVAALAARLSEAETARMALTVVERRPERVPLSFAQRRLWFIRQLEGPSATYN
                     VPIVVRLDGEVDVEALGAALRDVIERHEVLRTVFPVADGQPYQRILEPDELIWRMERVS
                     SADLEVAEAAGYAFDLASEVPIRAWLIDERVLVVVIHHIASDGWSRAPLARDVSVAYAA
                     RHDGRAPEWEPLPVQYADYALWQRELLGDDQDPDSVISRQVAYWRETLAGAPEELALP"
     CDS_motif       complement(93..131)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59310_0009"
                     /evalue="1.40E-06"
                     /label="C4_LCL_164-176"
                     /locus_tag="KLK06_RS59310"
                     /protein_end="780"
                     /protein_start="767"
                     /score="20.6"
                     /tool="antismash"
                     /translation="QYADYALWQRELL"
     CDS_motif       complement(180..245)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59310_0008"
                     /evalue="6.50E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="KLK06_RS59310"
                     /protein_end="751"
                     /protein_start="729"
                     /score="28.3"
                     /tool="antismash"
                     /translation="IHHIASDGWSRAPLARDVSVAY"
     CDS_motif       complement(423..539)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59310_0007"
                     /evalue="5.30E-20"
                     /label="C2_LCL_024-062"
                     /locus_tag="KLK06_RS59310"
                     /protein_end="670"
                     /protein_start="631"
                     /score="63.8"
                     /tool="antismash"
                     /translation="ATYNVPIVVRLDGEVDVEALGAALRDVIERHEVLRTVFP"
     CDS_motif       complement(561..590)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59310_0006"
                     /evalue="5.60E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="KLK06_RS59310"
                     /protein_end="624"
                     /protein_start="614"
                     /score="9.4"
                     /tool="antismash"
                     /translation="SFAQRRLWFI"
     aSDomain        complement(648..854)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59310_PCP.1"
                     /evalue="3.00E-28"
                     /label="KLK06_RS59310_PCP.1"
                     /locus_tag="KLK06_RS59310"
                     /protein_end="595"
                     /protein_start="526"
                     /score="89.8"
                     /tool="antismash"
                     /translation="EGLLCQAFAEVLGLSVVGVDDDFFALGGHSLLAVRLVSRVRALLG
                     VELGLSVLFEAPTVAALAARLSEA"
     aSModule        complement(648..2342)
                     /complete
                     /domains="nrpspksdomains_KLK06_RS59310_AMP-binding.1"
                     /domains="nrpspksdomains_KLK06_RS59310_PCP.1"
                     /locus_tags="KLK06_RS59310"
                     /monomer_pairings="Asn -> Asn"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(822..932)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59310_0005"
                     /evalue="3.80E+01"
                     /label="C2_LCL_024-062"
                     /locus_tag="KLK06_RS59310"
                     /protein_end="537"
                     /protein_start="500"
                     /score="-3.2"
                     /tool="antismash"
                     /translation="NGKLDRGALPVPEQGVGGGRVPVTVREGLLCQAFAEV"
     CDS_motif       complement(1137..1199)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59310_0004"
                     /evalue="1.10E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="KLK06_RS59310"
                     /protein_end="432"
                     /protein_start="411"
                     /score="36.6"
                     /tool="antismash"
                     /translation="FLGRADEQVKIRGFRVEPGEV"
     aSDomain        complement(1164..2342)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59310_AMP-binding.1"
                     /evalue="1.10E-95"
                     /label="KLK06_RS59310_AMP-binding.1"
                     /locus_tag="KLK06_RS59310"
                     /protein_end="423"
                     /protein_start="30"
                     /score="312.6"
                     /specificity="substrate consensus: Asn"
                     /tool="antismash"
                     /translation="FEGQVARRPDAVAVRSGGVAVSYAGVEERANRLAQVLVGEGVGVE
                     SVVGVCLGRGVDLVVALLAVWKVGAGYLPIDPGLPVERVAFMLADSGAVLTVTSAEIAE
                     ELPAGGGRMLVLDDALTVMRLSMASAERPERHVVGDQIAYVIYTSGSTGRPKGVVVTQA
                     GLANYVGWAAGFYGVGVGSVLHSSVAFDLTVTGLWVPLVLGAEVVVSEGGGVDGLVGVL
                     SEGLGLGLVKVVPAHLSLLAEVVSSDVGRTWVVGGEVLSGGLVRSWLARSPGSVVVNEY
                     GPTETVVGCCVFEASVEVGESVPIGRPIANCRLYVLDGWLRPVPPGVAGELYIAGVQVA
                     RGYVGRPGLSAERFVACPFELGGRMYRSGDVVRWGVDGDLEFLGRADEQVKIR"
     CDS_motif       complement(1281..1370)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59310_0003"
                     /evalue="3.00E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="KLK06_RS59310"
                     /protein_end="384"
                     /protein_start="354"
                     /score="51.5"
                     /tool="antismash"
                     /translation="PPGVAGELYIAGVQVARGYVGRPGLSAERF"
     CDS_motif       complement(1860..1916)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59310_0002"
                     /evalue="1.70E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="KLK06_RS59310"
                     /protein_end="191"
                     /protein_start="172"
                     /score="32.7"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVVVT"
     CDS_motif       complement(2115..2156)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59310_0001"
                     /evalue="2.40E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="KLK06_RS59310"
                     /protein_end="106"
                     /protein_start="92"
                     /score="14.5"
                     /tool="antismash"
                     /translation="LAVWKVGAGYLPID"
ORIGIN
        1 ggcagcgcca gctcctccgg cgccccagcc aaggtctccc gccagtaggc gacctgccgc
       61 gagatcacgc tgtccggatc ctggtcgtcc cccagcagtt cgcgctgcca cagggcgtag
      121 tcggcgtact ggacgggcag cggctcccac tccggagccc gcccgtcgtg ccgtgccgca
      181 taagccaccg acacatcccg cgccagcggt gcccgtgacc agccgtcact ggcgatgtgg
      241 tgaatcacca ccaccaacac ccgctcatcg atcaaccacg cccggatcgg cacctccgac
      301 gccagatcga aggcataccc cgccgcctcg gcgacctcca ggtccgccga cgacacccgc
      361 tccatccgcc agatcagctc atccggctcg agaatccgct ggtaaggctg accatcggcg
      421 accgggaaaa ccgtccgcaa cacctcatgc cgctcgatca catcccgcaa cgccgcccca
      481 agcgcctcga cgtccacctc accgtcgagc cgcaccacga tcggcacgtt ataagtggcg
      541 ctcgggcctt cgagctgcct gatgaaccac agacgccgct gcgcgaacga caacggcacc
      601 cgctccggcc gccgctccac caccgtcagc gccatccgag ccgtctcggc ctcgctcaac
      661 cgcgccgcca acgcggccac cgtcggcgcc tcgaacaaca ccgacaaccc caactccacc
      721 ccgagcagcg cccgcacccg gctgaccagc cgcaccgcca gcagcgaatg cccacccaaa
      781 gcaaagaaat cgtcgtccac ccccaccaca gacaacccca aaacctcggc gaacgcctga
      841 cacaacaagc cctcccgcac cgtcaccggc acccgcccac caccaacacc ctgctcaggc
      901 acaggcaaag ccccacgatc aagcttgccg ttactcgtca acggcaacgc ctcaagcgca
      961 accaccaccg acggcaccat gtactccggc aaccgctcca ccacaaaccg ccgcaccaga
     1021 tcagccaccc catcagcccc caccacatac gccaccaacc gcacatcacc cggcacatcc
     1081 tcacgagcca ccaccgccgc ctgagccacc agcggatgcg ccgccaccac cgcctgcacc
     1141 tcacccggct caacccggaa cccccgaatc ttcacctgct catcagcccg accaagaaac
     1201 tccaaatccc catccacacc ccaacgcaca acatcaccac tgcgatacat gcgccccccc
     1261 aactcaaacg ggcaagccac aaaccgctcc gcgctcagcc caggacgccc cacatacccc
     1321 cgagccacct gcacacccgc gatatacaac tcccccgcca cccccggcgg caccggccgc
     1381 aaccacccat ccagcacata caaccgacaa ttcgcaatcg gccgcccaat cggcaccgac
     1441 tccccaacct ccaccgacgc ctcaaacaca caacacccga ccaccgtctc cgtcggacca
     1501 tactcattaa ccaccaccga ccccggcgac cgcgccaacc acgaacgcac caaccccccc
     1561 gacaacacct caccacccac cacccacgtc cgccccacat cagacgacac cacctcagcc
     1621 agcaaactca gatgcgccgg caccaccttc accaaaccca aacccaaacc ctcagacaac
     1681 acccccacca acccatcaac accacccccc tcactcacca ccacctccgc acccaacacc
     1741 aacggcaccc acaaacccgt caccgtcaaa tcaaacgcca ccgacgaatg caacacagaa
     1801 cccacaccca ccccataaaa cccagccgcc caacccacat aattagccaa ccccgcctgc
     1861 gtcaccacca cccccttcgg ccgccccgtc gaacccgacg tataaatgac ataggcgatc
     1921 tgatcgccga ctacgtgccg ctccggtctt tcggccgagg ccatcgacag ccgcatcacg
     1981 gtcaaggcgt catccagcac caacatccga cccccacccg ccggcagctc ctcagcaatc
     2041 tccgcactgg taaccgtcaa caccgcacca ctgtcagcaa gcatgaacgc gacccgctcc
     2101 accggcagcc ccggatcaat cggaagataa cccgccccca ccttccacac cgccagcaac
     2161 gccaccacca gatccacccc acgccccaaa cacaccccca ccaccgactc cacacccacc
     2221 ccctcaccaa ccaacacctg agccaaccga ttcgcccgct cctccacccc cgcatacgac
     2281 accgccaccc cacccgaccg caccgccacc gcatccggcc tccgagccac ctgcccctca
     2341 aacaactcca ccaccgacac accacccacc tccacagcag tggcattcca cccctccagc
     2401 accaaccgcc gctccacctc atcgagcaca tc
//
