LOCUS       NZ_JAHFZZ010000063      2881 bp    DNA     linear   CON 13-SEP-2024
DEFINITION  Nonomuraea sp. NEAU-A123 Scaffold63, whole genome shotgun sequence.
ACCESSION   NZ_JAHFZZ010000063
VERSION     NZ_JAHFZZ010000063.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nonomuraea sp. NEAU-A123
  ORGANISM  Nonomuraea sp. NEAU-A123
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Nonomuraea.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Zhang,L. and Zhao,J.
  TITLE     Nonomuraea triticisoli sp. nov., a novel actinobacterium isolated
            from rhizospheric soil of wheat in Langfang, Hebei Province, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Zhang,L. and Zhao,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2021) College of Life Sciences, Northeast
            Agricultural University, Changjiang Road No. 600, Xiangfang
            District, Harbin, Heilongjiang Province 150036, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018603905.1-RS_2024_09_13
            Annotation Date                   :: 09/13/2024 04:26:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11,983
            CDSs (total)                      :: 11,901
            Genes (coding)                    :: 11,761
            CDSs (with protein)               :: 11,761
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 28 of 140
            Pseudo Genes (incomplete)         :: 121 of 140
            Pseudo Genes (internal stop)      :: 3 of 140
            Pseudo Genes (multiple problems)  :: 12 of 140
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:52:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2881
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHFZZ010000063.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2881
                     /collection_date="2016-05-10"
                     /db_xref="taxon:2839649"
                     /geo_loc_name="China: Langfang"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Nonomuraea sp. NEAU-A123"
                     /strain="NEAU-A123"
                     /submitter_seqid="Scaffold63"
     region          1..2881
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2881
                     /SMILES="NC(CO)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2881
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[20:>2881](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      21..>2881
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            21..>2881
                     /locus_tag="KLK06_RS59295"
                     /old_locus_tag="KLK06_59300"
     CDS             21..>2881
                     /NRPS_PKS="Domain: AMP-binding (61-457). E-value: 7.7e-106.
                     Score: 346.0. Matches aSDomain:
                     nrpspksdomains_KLK06_RS59295_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (561-628). E-value: 5e-23. Score:
                     73.1. Matches aSDomain: nrpspksdomains_KLK06_RS59295_PCP.1"
                     /NRPS_PKS="Domain: Condensation_Dual (675-953). E-value:
                     2.3e-105. Score: 344.1. Matches aSDomain:
                     nrpspksdomains_KLK06_RS59295_Condensation_Dual.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /locus_tag="KLK06_RS59295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_59300"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_214644731.1"
                     /sec_met_domain="AMP-binding (E-value: 1e-115, bitscore:
                     372.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.1e-16, bitscore:
                     45.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 6.8e-41, bitscore:
                     126.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGLLVRTATGNLVAALEQALDGANEPPLSSVDVLDDEERRLVVEE
                     WNDTAGEVLSGSLVGLFEAQVGRSPGAVAVVGPDGERVSFAELDGRANRLARLLVGQGV
                     VAESVVGVCLERGVDLVVALLGVLKAGAAYLPVDPEYPAERVRMLLEDAGARWLITGGG
                     VRPGLPEGVQVVDLDDPAVAAGLPGGRLGAVPADSAAYVIFTSGSTGRPKGVVVSHSGV
                     VNRLAWMQERLGLVLGEGVLQKTSFGFDVSVWEFFWPLTQGGVLVLARPGGQRDASYLA
                     GLIQAEHVRVAHFVPSMLEAFLAEPAAASCGGLRWVVCSGEALPVHVMRRFFEVLDGVG
                     LENYYGPTEASIDVTAWRCTPDWDGASVPIGRPVANTRAYVLDERLSPVPVGVVGDLYV
                     SGIQLARGYTGRPGLTAERFVACPFGGGRMYRTGDRVSWSAEGELLFAGRADDQVKIRG
                     FRVEPGEVASVLAECPGVGQVAVVAREDVPGDVRLVAYVVLSGEISVDVVREFAAARLP
                     EYMVPSAVVTLDALPVSVNGKLDRKALPAPQYTAGTGRAPANPREEVLSAAFAEILGLD
                     GVGVDDDFFRLGGHSLLVIRLVELLRSRGVPVSVRALFETPTVAGLAAAAGSRQVSVPE
                     NLIPAGATAITPEMLPLVDLTADEIGRVVATVEGGAANVADVYPLAPLQEGLLFHHLLA
                     DGGEDAYVLRAVLEFDSRERVERFVEALQRVVDRHDIYRTSLVWEGLRESVQVVWRQAV
                     LPVREVVLDPDGADPVGELVALAGSVMDLRWAPLMGVHLAAVPGGGRWLALVRMHHVVR
                     DHTALEVLLAEVAAFLAGRGAELAVPLPFRNFVVQARQGITDDEHHRYFAELLGDVEEP
                     TAPFGLVDVRGDGSDSAHEVVPFAGDLHRRLREVARRLGVSPATVLHVAWARVLEVVSG
                     RDDVVFGTVLFGRMNAGAGADRVP"
     aSDomain        204..1391
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59295_AMP-binding.1"
                     /evalue="7.70E-106"
                     /label="KLK06_RS59295_AMP-binding.1"
                     /locus_tag="KLK06_RS59295"
                     /protein_end="457"
                     /protein_start="61"
                     /score="346.0"
                     /specificity="substrate consensus: Ser"
                     /tool="antismash"
                     /translation="FEAQVGRSPGAVAVVGPDGERVSFAELDGRANRLARLLVGQGVVA
                     ESVVGVCLERGVDLVVALLGVLKAGAAYLPVDPEYPAERVRMLLEDAGARWLITGGGVR
                     PGLPEGVQVVDLDDPAVAAGLPGGRLGAVPADSAAYVIFTSGSTGRPKGVVVSHSGVVN
                     RLAWMQERLGLVLGEGVLQKTSFGFDVSVWEFFWPLTQGGVLVLARPGGQRDASYLAGL
                     IQAEHVRVAHFVPSMLEAFLAEPAAASCGGLRWVVCSGEALPVHVMRRFFEVLDGVGLE
                     NYYGPTEASIDVTAWRCTPDWDGASVPIGRPVANTRAYVLDERLSPVPVGVVGDLYVSG
                     IQLARGYTGRPGLTAERFVACPFGGGRMYRTGDRVSWSAEGELLFAGRADDQVKIR"
     aSModule        204..1904
                     /complete
                     /domains="nrpspksdomains_KLK06_RS59295_AMP-binding.1"
                     /domains="nrpspksdomains_KLK06_RS59295_PCP.1"
                     /locus_tags="KLK06_RS59295"
                     /monomer_pairings="Ser -> Ser"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       393..434
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59295_0001"
                     /evalue="1.80E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="KLK06_RS59295"
                     /protein_end="138"
                     /protein_start="124"
                     /score="17.8"
                     /tool="antismash"
                     /translation="LGVLKAGAAYLPVD"
     CDS_motif       618..677
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59295_0002"
                     /evalue="3.20E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="KLK06_RS59295"
                     /protein_end="219"
                     /protein_start="199"
                     /score="34.9"
                     /tool="antismash"
                     /translation="AYVIFTSGSTGRPKGVVVSH"
     CDS_motif       1188..1277
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59295_0003"
                     /evalue="2.30E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="KLK06_RS59295"
                     /protein_end="419"
                     /protein_start="389"
                     /score="48.7"
                     /tool="antismash"
                     /translation="PVGVVGDLYVSGIQLARGYTGRPGLTAERF"
     CDS_motif       1356..1418
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59295_0004"
                     /evalue="1.50E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="KLK06_RS59295"
                     /protein_end="466"
                     /protein_start="445"
                     /score="36.2"
                     /tool="antismash"
                     /translation="FAGRADDQVKIRGFRVEPGEV"
     aSDomain        1704..1904
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59295_PCP.1"
                     /evalue="5.00E-23"
                     /label="KLK06_RS59295_PCP.1"
                     /locus_tag="KLK06_RS59295"
                     /protein_end="628"
                     /protein_start="561"
                     /score="73.1"
                     /tool="antismash"
                     /translation="EEVLSAAFAEILGLDGVGVDDDFFRLGGHSLLVIRLVELLRSRGV
                     PVSVRALFETPTVAGLAAAAGS"
     aSDomain        2046..2879
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59295_Condensation_Dual.
                     1"
                     /domain_subtypes="Condensation_Dual"
                     /evalue="2.30E-105"
                     /label="KLK06_RS59295_Condensation_Dual.1"
                     /locus_tag="KLK06_RS59295"
                     /protein_end="953"
                     /protein_start="675"
                     /score="344.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHVVRDH"
                     /tool="antismash"
                     /translation="DVYPLAPLQEGLLFHHLLADGGEDAYVLRAVLEFDSRERVERFVE
                     ALQRVVDRHDIYRTSLVWEGLRESVQVVWRQAVLPVREVVLDPDGADPVGELVALAGSV
                     MDLRWAPLMGVHLAAVPGGGRWLALVRMHHVVRDHTALEVLLAEVAAFLAGRGAELAVP
                     LPFRNFVVQARQGITDDEHHRYFAELLGDVEEPTAPFGLVDVRGDGSDSAHEVVPFAGD
                     LHRRLREVARRLGVSPATVLHVAWARVLEVVSGRDDVVFGTVLFGRMNAGAGADRV"
     CDS_motif       2052..2093
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59295_0005"
                     /evalue="5.20E-07"
                     /label="C1_dual_004-017"
                     /locus_tag="KLK06_RS59295"
                     /protein_end="691"
                     /protein_start="677"
                     /score="21.9"
                     /tool="antismash"
                     /translation="YPLAPLQEGLLFHH"
     CDS_motif       2121..2234
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59295_0006"
                     /evalue="2.90E-19"
                     /label="C2_dual_024-063"
                     /locus_tag="KLK06_RS59295"
                     /protein_end="738"
                     /protein_start="700"
                     /score="61.6"
                     /tool="antismash"
                     /translation="YVLRAVLEFDSRERVERFVEALQRVVDRHDIYRTSLVW"
     CDS_motif       2439..2504
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59295_0007"
                     /evalue="5.50E-06"
                     /label="C3_dual_135-156"
                     /locus_tag="KLK06_RS59295"
                     /protein_end="828"
                     /protein_start="806"
                     /score="19.3"
                     /tool="antismash"
                     /translation="MHHVVRDHTALEVLLAEVAAFL"
     CDS_motif       2538..2576
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59295_0008"
                     /evalue="3.60E-04"
                     /label="C4_dual_169-182"
                     /locus_tag="KLK06_RS59295"
                     /protein_end="852"
                     /protein_start="839"
                     /score="13.4"
                     /tool="antismash"
                     /translation="PFRNFVVQARQGI"
     CDS_motif       2817..2876
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59295_0009"
                     /evalue="1.30E-07"
                     /label="C5_dual_260-291"
                     /locus_tag="KLK06_RS59295"
                     /protein_end="952"
                     /protein_start="932"
                     /score="24.4"
                     /tool="antismash"
                     /translation="DVVFGTVLFGRMNAGAGADR"
ORIGIN
        1 ccccgatcga tgctcggagc gtgggactgc tggtccgcac cgccaccggc aacctcgtgg
       61 ccgcgctgga gcaggcgctg gacggcgcga acgagccgcc gctgagcagc gtggacgtgc
      121 tcgacgacga ggagcggcgc ctggtggtgg aggagtggaa cgacacggct ggtgaggtgt
      181 tgtcggggtc gttggtgggg ttgttcgagg cgcaggtggg gcggtcgcct ggggcggtgg
      241 cggtggtggg gccggacggt gagcgggtgt cgtttgcgga gttggatggg cgggcgaatc
      301 ggttggcgcg gttgctggtc gggcaggggg tggtggcgga gtcggtggtg ggggtgtgtt
      361 tggagcgtgg ggtggatctg gtggtggcgt tgctgggggt gttgaaggcg ggggccgcgt
      421 atctgccggt ggatccggag tatccggccg agcgggtccg gatgctgctg gaggatgcgg
      481 gggcgcggtg gctgatcact gggggtggcg tcaggccagg gctgccggag ggggtgcagg
      541 tcgttgacct ggatgacccg gcagtggcgg cggggctgcc tggtgggcgg ctgggtgcgg
      601 tgccggccga cagtgcggcg tatgtcatct tcacctcggg ttcgacgggg cggcccaagg
      661 gtgtggtggt gtcgcactca ggggtggtca accggctggc ctggatgcag gaacgcttgg
      721 ggttggtgct gggtgagggg gtgttgcaga agacgtcgtt cgggttcgac gtgtcggtgt
      781 gggagttctt ctggcctctg acgcagggcg gggtgctggt gctggcgcgg ccgggtggtc
      841 agcgggacgc ctcctatctg gctgggctga tccaggccga gcacgtgcgg gtggcgcatt
      901 ttgtgccgtc gatgctggag gcgttcctgg ccgagcccgc cgccgcctcg tgtggcggcc
      961 tgcggtgggt ggtgtgctct ggtgaagccc tgccggtgca tgtgatgcgg cgctttttcg
     1021 aggtcctgga cggggtgggc ctggagaact actacggccc gaccgaggcg tccatcgacg
     1081 tgaccgcctg gcggtgcaca ccggattggg acggggcttc ggtgccgatc ggccggccgg
     1141 tggccaacac ccgggcgtac gtgctggacg agcggctgtc tccggtgccg gtcggagtgg
     1201 tgggcgacct gtatgtgtcg gggatccaac tcgcacgtgg ctacaccggt cgccccggcc
     1261 tgaccgccga gcggttcgtg gcgtgcccgt tcggcggcgg gcggatgtac cggaccgggg
     1321 accgggtgtc ctggtcggcc gagggcgagc tgctgttcgc cggccgtgcg gacgaccagg
     1381 tgaagatccg gggctttcgc gtcgagccgg gcgaggtcgc gtcggtgctg gccgaatgcc
     1441 ctggggtggg gcaggtcgcg gtggtggcgc gggaggacgt gcctggggac gtgcggctgg
     1501 tggcctatgt ggtgctatcg ggcgaaattt cggttgatgt cgtacgggag ttcgccgccg
     1561 cgcggttgcc cgagtacatg gtgccgtcgg cggtggtgac actggatgcc ctgcccgtgt
     1621 cggtcaacgg caagctcgac cgcaaggcgc tgcccgcccc tcagtacacc gcgggcaccg
     1681 gccgggcccc ggccaacccg cgcgaggagg tgctgagcgc ggccttcgcc gagatcctgg
     1741 ggctggacgg cgtgggggtg gatgacgact tcttccggct gggtgggcat tcgctcctgg
     1801 tgatccggct ggtggagctg ctgaggtcgc gtggcgtgcc ggtgtcggtg cgggcgttgt
     1861 tcgagacacc gacggtggcc ggactggccg cggcggccgg gtcccggcag gtgagcgttc
     1921 cggagaacct gatcccggcc ggcgcgaccg ccatcacgcc ggagatgttg cccctggtgg
     1981 acctgaccgc cgacgagatc gggcgggtgg tggccaccgt cgagggcggc gcggcgaacg
     2041 tggccgacgt ctatccgctg gcgccgttgc aggaagggct gctgttccac cacctgctgg
     2101 ccgacggcgg cgaggacgcg tacgtgctgc gggcggtgct cgagttcgat tcccgggagc
     2161 gggtggagcg gttcgtcgag gcattgcagc gggtcgtgga tcggcacgac atctatcgca
     2221 cgtccctggt gtgggagggg ttgcgggagt cggtgcaggt ggtgtggcgt caggccgtgc
     2281 tgccggtgcg ggaggtggtg ctcgatccgg atggcgccga tcccgtcggt gagctggtgg
     2341 cgctggcggg gtcggtcatg gatctgcgtt gggcgccgtt gatgggtgtg cacctggcgg
     2401 cggtgccggg gggcgggcgg tggctggcgc tcgtccggat gcatcacgtg gtgcgtgacc
     2461 ataccgcgtt ggaggtgctg ctcgccgagg tggcggcgtt cctggccggt cggggggcgg
     2521 aattggccgt gccgctgccg ttccggaact tcgtggtgca ggcgcgtcag gggatcactg
     2581 acgatgagca tcatcgctat ttcgcggagt tgctgggtga tgtggaggag ccgacggcgc
     2641 cgtttgggct ggtggatgtg cgcggcgacg gcagcgactc cgcgcatgag gtggtgccgt
     2701 tcgccggtga tctgcaccgg cggctgcggg aggtggccag gcggctgggc gtgagtccgg
     2761 cgacggtgct gcatgtggcg tgggcccggg tgttggaagt ggtctccggc cgggacgatg
     2821 tggtgttcgg cacggtgctg ttcggccgga tgaacgccgg cgccggtgcg gaccgcgtgc
     2881 c
//
