LOCUS       NZ_JAHFZZ010000059      4477 bp    DNA     linear   CON 13-SEP-2024
DEFINITION  Nonomuraea sp. NEAU-A123 Scaffold59, whole genome shotgun sequence.
ACCESSION   NZ_JAHFZZ010000059
VERSION     NZ_JAHFZZ010000059.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nonomuraea sp. NEAU-A123
  ORGANISM  Nonomuraea sp. NEAU-A123
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Nonomuraea.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Zhang,L. and Zhao,J.
  TITLE     Nonomuraea triticisoli sp. nov., a novel actinobacterium isolated
            from rhizospheric soil of wheat in Langfang, Hebei Province, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Zhang,L. and Zhao,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2021) College of Life Sciences, Northeast
            Agricultural University, Changjiang Road No. 600, Xiangfang
            District, Harbin, Heilongjiang Province 150036, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018603905.1-RS_2024_09_13
            Annotation Date                   :: 09/13/2024 04:26:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11,983
            CDSs (total)                      :: 11,901
            Genes (coding)                    :: 11,761
            CDSs (with protein)               :: 11,761
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 28 of 140
            Pseudo Genes (incomplete)         :: 121 of 140
            Pseudo Genes (internal stop)      :: 3 of 140
            Pseudo Genes (multiple problems)  :: 12 of 140
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:52:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4477
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHFZZ010000059.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4477
                     /collection_date="2016-05-10"
                     /db_xref="taxon:2839649"
                     /geo_loc_name="China: Langfang"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Nonomuraea sp. NEAU-A123"
                     /strain="NEAU-A123"
                     /submitter_seqid="Scaffold59"
     gene            <1..>4477
                     /locus_tag="KLK06_RS59245"
                     /old_locus_tag="KLK06_59250"
     region          1..4477
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..4477
                     /SMILES="NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4477
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:>4477](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>4477
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             1..>4477
                     /NRPS_PKS="Domain: Condensation_LCL (9-190). E-value:
                     4.4e-74. Score: 241.1. Matches aSDomain:
                     nrpspksdomains_KLK06_RS59245_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (360-744). E-value:
                     2.8e-105. Score: 344.2. Matches aSDomain:
                     nrpspksdomains_KLK06_RS59245_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (847-915). E-value: 3.1e-28. Score:
                     89.8. Matches aSDomain: nrpspksdomains_KLK06_RS59245_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (929-1225). E-value:
                     3.9e-108. Score: 352.9. Matches aSDomain:
                     nrpspksdomains_KLK06_RS59245_Condensation_LCL.2"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="KLK06_RS59245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_59250"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_214644723.1"
                     /sec_met_domain="AMP-binding (E-value: 3.2e-113, bitscore:
                     364.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.7e-19, bitscore:
                     56.4, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.5e-65, bitscore:
                     206.6, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVPIRAWLIDERVLVVVIHHIASDGWSRAPLARDVSVAYAARHDG
                     RAPEWEPLPVQYADYALWQRELLGDDQDPDSVISRQVAYWRETLAGAPEELALPADRAR
                     PAVTSYRGYTVPIEVPAEVHARLAEVAQAEGVTTFMVVQAALAVLLSRLGAGTDIPIGA
                     ANAGRTDEALDDLVGFFINTLVLRTDLSGNPTFREVLGRVRESSLAAFAHQDVPFERLV
                     EELAPSRSMARHPLFQVMLTLENTGEAVLELPGVGARRMSAGAAVAKFDLEVLLGEVFD
                     AHGGPAGVRGSLVASADLFEGETAERLTGRLTRVLASLTAEPQTRLSAVEVLDAGERRR
                     VLTGWNDTAGEVAGSSLVDLFQAQVRRTPQALAVVGEGVRVSYAELDERADRLARALAA
                     EGVVPESVVGVCVGRGLEWVVAVLGVLKAGGAFLPVDPVYPAERIAYSMADAGAVAVVT
                     SPALAHLVPDGLTRVMVDDPAAHPGGPLDVRLVPESAAYVIYTSGSTGRPKGVVVTHGG
                     AVNLLAAGGWEAGEGDRVLQFASPGFDAACWELLAALWSGGCLVVADSAELLPGAGLAG
                     VIQRYGVSHLLVPPSLLGVMGTAELAGVRTVVAGGEALGSELVARWGAGRRLVNAYGPT
                     EVTVCAAMTDDLFADREVTIGGPHVNVRAYVLDEWLCPVPPGVVGELYLAGAQVARGYA
                     GRPGLTGERFVADRFGSAGGRMYRTGDRVRWDETGRLVFAGRGDDQVKIRGFRIEPGEV
                     QAVLAAHPQVTQAAVVVREDVPGDKRLVAYLAPQEVDTDSVRAMAAEQLPAHMVPAALV
                     TLAALPVTTSGKLDRKALPAPDYGGAAGRGPADAREELLCGVFAQVLGLPQVAVDDDFF
                     ALGGHSLLAVRLISRVRSVLGVEVPLRALFEAPTVARLAERLTGAGRARPALTPLERPE
                     RVPLSFAQRRLWFIGQLEGPSATYNVPIVVRLGGDVDVEALGAALRDVIERHEVLRTVF
                     PVADGEPYQQVVGMDELHWELSVGEARDGLEGAVTEAVGHAFDLSSEVPIRAWLFDGSV
                     LVVVVHHIASDGWSMAPLARDFSLAYAARRAGRAPSWDALPVQYADYALWQRDLLGAED
                     DPGSLISTQVAYWREALAGAPEELALPFDRPRPVVASHRGHSVPLLVPAEVHVRLVEVA
                     RAEGVTVFMVLQAALAVLLSRLGAGTDVSIGSANAGRVDVALEDLVGCFVNTLVVRADL
                     SGDPEFRALLGRVRERSLAAFAHQDVPFERLVEELAPSRSMARHPLFQVVLTKQDTIAA
                     ELDLPGVRTGPAPVAGTGRTAAKFDLDVMVSEVFDGEGVPAGVRGSVTVATDLFDPEWA
                     ERIARYWVRVLEVVGTDTRTRLSEVDLLEEHERRRVVEEWNSTAAEKTSAPVVELFEGQ
                     VARRPEAVALVGPELVTYAELDGRANRLAHVLIGEGVGGESVVGVCLDRGVELIVAVLA
                     VWKAGAAYVPIDPRQPVERIAYMLGDSRAVL"
     aSDomain        28..570
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59245_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="4.40E-74"
                     /label="KLK06_RS59245_Condensation_LCL.1"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="190"
                     /protein_start="9"
                     /score="241.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIASDG"
                     /tool="antismash"
                     /translation="DERVLVVVIHHIASDGWSRAPLARDVSVAYAARHDGRAPEWEPLP
                     VQYADYALWQRELLGDDQDPDSVISRQVAYWRETLAGAPEELALPADRARPAVTSYRGY
                     TVPIEVPAEVHARLAEVAQAEGVTTFMVVQAALAVLLSRLGAGTDIPIGAANAGRTDEA
                     LDDLVGFFINTLVLRTDL"
     aSModule        28..2745
                     /complete
                     /domains="nrpspksdomains_KLK06_RS59245_Condensation_LCL.1"
                     /domains="nrpspksdomains_KLK06_RS59245_AMP-binding.1"
                     /domains="nrpspksdomains_KLK06_RS59245_PCP.1"
                     /locus_tags="KLK06_RS59245"
                     /monomer_pairings="Leu -> Leu"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       52..117
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0001"
                     /evalue="1.30E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="39"
                     /protein_start="17"
                     /score="27.4"
                     /tool="antismash"
                     /translation="IHHIASDGWSRAPLARDVSVAY"
     CDS_motif       166..204
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0002"
                     /evalue="2.70E-06"
                     /label="C4_LCL_164-176"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="68"
                     /protein_start="55"
                     /score="19.7"
                     /tool="antismash"
                     /translation="QYADYALWQRELL"
     CDS_motif       472..561
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0003"
                     /evalue="3.20E-12"
                     /label="C5_LCL_267-296"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="187"
                     /protein_start="157"
                     /score="38.8"
                     /tool="antismash"
                     /translation="DIPIGAANAGRTDEALDDLVGFFINTLVLR"
     CDS_motif       610..729
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0004"
                     /evalue="2.30E-22"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="243"
                     /protein_start="203"
                     /score="71.4"
                     /tool="antismash"
                     /translation="RESSLAAFAHQDVPFERLVEELAPSRSMARHPLFQVMLTL"
     aSDomain        1081..2232
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59245_AMP-binding.1"
                     /evalue="2.80E-105"
                     /label="KLK06_RS59245_AMP-binding.1"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="744"
                     /protein_start="360"
                     /score="344.2"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="FQAQVRRTPQALAVVGEGVRVSYAELDERADRLARALAAEGVVPE
                     SVVGVCVGRGLEWVVAVLGVLKAGGAFLPVDPVYPAERIAYSMADAGAVAVVTSPALAH
                     LVPDGLTRVMVDDPAAHPGGPLDVRLVPESAAYVIYTSGSTGRPKGVVVTHGGAVNLLA
                     AGGWEAGEGDRVLQFASPGFDAACWELLAALWSGGCLVVADSAELLPGAGLAGVIQRYG
                     VSHLLVPPSLLGVMGTAELAGVRTVVAGGEALGSELVARWGAGRRLVNAYGPTEVTVCA
                     AMTDDLFADREVTIGGPHVNVRAYVLDEWLCPVPPGVVGELYLAGAQVARGYAGRPGLT
                     GERFVADRFGSAGGRMYRTGDRVRWDETGRLVFAGRGDDQVKIR"
     CDS_motif       1267..1308
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0005"
                     /evalue="1.30E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="436"
                     /protein_start="422"
                     /score="15.3"
                     /tool="antismash"
                     /translation="LGVLKAGGAFLPVD"
     CDS_motif       1486..1545
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0006"
                     /evalue="4.60E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="515"
                     /protein_start="495"
                     /score="34.4"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVVVTH"
     CDS_motif       1651..1686
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0007"
                     /evalue="2.30E+01"
                     /label="NRPS-A_a2"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="562"
                     /protein_start="550"
                     /score="-0.4"
                     /tool="antismash"
                     /translation="LAALWSGGCLVV"
     CDS_motif       2023..2112
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0008"
                     /evalue="1.30E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="704"
                     /protein_start="674"
                     /score="46.3"
                     /tool="antismash"
                     /translation="PPGVVGELYLAGAQVARGYAGRPGLTGERF"
     CDS_motif       2197..2259
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0009"
                     /evalue="3.80E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="753"
                     /protein_start="732"
                     /score="35.0"
                     /tool="antismash"
                     /translation="FAGRGDDQVKIRGFRIEPGEV"
     aSDomain        2542..2745
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59245_PCP.1"
                     /evalue="3.10E-28"
                     /label="KLK06_RS59245_PCP.1"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="915"
                     /protein_start="847"
                     /score="89.8"
                     /tool="antismash"
                     /translation="EELLCGVFAQVLGLPQVAVDDDFFALGGHSLLAVRLISRVRSVLG
                     VEVPLRALFEAPTVARLAERLTG"
     aSDomain        2788..3675
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59245_Condensation_LCL.2
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.90E-108"
                     /label="KLK06_RS59245_Condensation_LCL.2"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="1225"
                     /protein_start="929"
                     /score="352.9"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIASDG"
                     /tool="antismash"
                     /translation="ERVPLSFAQRRLWFIGQLEGPSATYNVPIVVRLGGDVDVEALGAA
                     LRDVIERHEVLRTVFPVADGEPYQQVVGMDELHWELSVGEARDGLEGAVTEAVGHAFDL
                     SSEVPIRAWLFDGSVLVVVVHHIASDGWSMAPLARDFSLAYAARRAGRAPSWDALPVQY
                     ADYALWQRDLLGAEDDPGSLISTQVAYWREALAGAPEELALPFDRPRPVVASHRGHSVP
                     LLVPAEVHVRLVEVARAEGVTVFMVLQAALAVLLSRLGAGTDVSIGSANAGRVDVALED
                     LVGCFVNTLVVRADL"
     CDS_motif       2854..2970
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0010"
                     /evalue="1.20E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="990"
                     /protein_start="951"
                     /score="59.5"
                     /tool="antismash"
                     /translation="ATYNVPIVVRLGGDVDVEALGAALRDVIERHEVLRTVFP"
     CDS_motif       3157..3222
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0011"
                     /evalue="3.80E-10"
                     /label="C3_LCL_132-143"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="1074"
                     /protein_start="1052"
                     /score="32.2"
                     /tool="antismash"
                     /translation="VHHIASDGWSMAPLARDFSLAY"
     CDS_motif       3271..3309
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0012"
                     /evalue="1.00E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="1103"
                     /protein_start="1090"
                     /score="18.0"
                     /tool="antismash"
                     /translation="QYADYALWQRDLL"
     CDS_motif       3577..3666
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0013"
                     /evalue="8.30E-11"
                     /label="C5_LCL_267-296"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="1222"
                     /protein_start="1192"
                     /score="34.3"
                     /tool="antismash"
                     /translation="DVSIGSANAGRVDVALEDLVGCFVNTLVVR"
     CDS_motif       3715..3828
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0014"
                     /evalue="8.50E-21"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="1276"
                     /protein_start="1238"
                     /score="66.4"
                     /tool="antismash"
                     /translation="RERSLAAFAHQDVPFERLVEELAPSRSMARHPLFQVVL"
     CDS_motif       4378..4419
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59245_0015"
                     /evalue="2.30E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="KLK06_RS59245"
                     /protein_end="1473"
                     /protein_start="1459"
                     /score="20.5"
                     /tool="antismash"
                     /translation="LAVWKAGAAYVPID"
ORIGIN
        1 gaggtgccga tccgggcgtg gttgatcgat gagcgggtgt tggtggtggt gattcaccac
       61 atcgccagtg acggctggtc acgggcaccg ctggcgcggg atgtgtcggt ggcttatgcg
      121 gcacggcacg acgggcgggc tccggagtgg gagccgctgc ccgtccagta cgccgactac
      181 gccctgtggc agcgcgaact gctgggggac gaccaggatc cggacagcgt gatctcgcgg
      241 caggtcgcct actggcggga gaccttggct ggggcgccgg aggagctggc gctgccggcc
      301 gaccgggccc gccctgccgt gacctcctat cgcgggtaca cggtgccgat cgaggtgccg
      361 gccgaggtgc acgcgcgcct ggcggaggtg gcgcaggccg agggcgtcac cacattcatg
      421 gtggtccagg ccgcgctggc ggtgctgctg tcacggctgg gcgccggaac cgacatcccc
      481 atcggcgcgg ccaacgccgg ccgcaccgat gaggccctgg acgatctcgt cgggttcttc
      541 atcaacactc tggtgctgcg taccgacctg tctggaaacc cgacgttccg cgaggtgctc
      601 ggccgcgtac gcgagtccag cctggcggcg ttcgcgcatc aggacgtgcc gttcgagcgg
      661 ctggtcgagg agctggcgcc gtcgcggtcc atggcgcggc acccgctgtt ccaggtgatg
      721 ctgaccctgg agaacaccgg cgaggccgtg ctggagctgc ccggggtggg ggcccggcgg
      781 atgtcggccg gtgcggcggt ggcgaagttc gacctcgagg tgctgctcgg tgaggtgttc
      841 gacgcccacg gcgggcccgc gggggtgcgc ggctcgctgg tggcgtccgc ggacctcttc
      901 gagggggaga cggccgagcg gctgaccggg cggctgacgc gggtgctggc gtcgctgacc
      961 gccgagccgc agacccggct gagcgccgtg gaggtgctcg acgccggcga acggcgacgc
     1021 gtgctgaccg ggtggaacga caccgccggt gaggtggccg gctcgtcgct ggtggacctc
     1081 ttccaggcac aggtacggcg gacgccccag gccctcgcgg tggtcgggga gggtgtccgg
     1141 gtgtcgtacg ccgagttgga cgaacgggcc gaccggctgg cccgcgcgct ggcggccgag
     1201 ggtgtggtgc ccgagtcggt ggtgggggtg tgcgtcggcc ggggcctcga atgggtcgtg
     1261 gcggtgctgg gcgtgctgaa ggccggcggt gctttcctgc cagtggatcc ggtgtacccg
     1321 gctgagcgga tcgcgtactc gatggcggac gcgggcgcgg tggcggtggt gacctcgccg
     1381 gccctggccc acctggtgcc ggacggcctg acgcgggtga tggtggacga cccggcggcg
     1441 cacccgggcg gacccttgga cgtgcggctg gtgcccgagt ccgcggcata cgtcatctac
     1501 acctccggat cgaccggccg gcccaaaggc gtggtagtca cccacggagg cgcggtgaac
     1561 ctgctggcgg ccggcggctg ggaggccggc gagggcgacc gcgtgctgca gttcgcctcg
     1621 cccggcttcg acgcggcctg ctgggaactg ctggcggcgc tgtggtcggg cggctgcctc
     1681 gtggtggccg acagcgcgga actgctgccg ggggccgggc tggccggggt gatccagcgg
     1741 tacggggtgt cgcacctact ggtgccgccc tcgctcctgg gcgtgatggg cacggcggaa
     1801 ctggccgggg tacggaccgt ggtggccggc ggggaggccc tcggctcgga gctcgtggcg
     1861 cgctggggag ccgggcgccg gctcgtcaac gcgtacgggc cgaccgaggt gaccgtgtgc
     1921 gcggcgatga ccgatgacct gttcgcggac cgcgaggtga ccatcggcgg gccgcacgtg
     1981 aacgtgcgcg cctacgtgct cgacgagtgg ctgtgcccgg tgccgccggg cgtggtgggc
     2041 gagctgtacc tggccggggc acaggtggcc cgcggctacg cggggcgtcc cgggctgacc
     2101 ggggagcggt tcgtggccga tcggttcggg tcggcgggcg gacggatgta ccggaccggg
     2161 gaccgggtgc gctgggacga gaccggccgc ctggtgttcg ccggccgcgg tgacgaccag
     2221 gtgaagatcc gcgggttccg gatcgagccg ggcgaggtgc aggcggtgct cgccgcccac
     2281 ccccaggtga cgcaggcggc ggtcgtcgtg cgcgaggacg tgcccggaga caagcggctg
     2341 gtcgcctatc tcgcaccgca ggaggtggac accgactcgg tacgggcgat ggccgccgaa
     2401 cagctgcccg cccacatggt gccggccgcg ctcgtgaccc tcgccgccct gcccgtcacc
     2461 accagcggca agctcgaccg caaggccctg cccgcccccg actacggcgg cgcggcggga
     2521 cgcggcccgg ccgacgcccg cgaggaactc ctgtgcgggg tgttcgcaca ggtgctgggg
     2581 ctgccgcagg tggcggtcga cgacgacttc ttcgcgctgg gcgggcattc cctgctggcg
     2641 gtacggctca tcagccgcgt ccggtcggtg ctgggcgtgg aggtgccgtt gcgggccctg
     2701 ttcgaggcgc cgacggtggc ccgcctggcg gagcggctga ccggcgccgg gcgggcccgg
     2761 cctgcgctga cgccgctgga gcggccggag cgggttccgt tgtcgttcgc gcagcggcgg
     2821 ttgtggttca tcggccagct cgaggggccg agcgcgacct acaacgtgcc gatcgtggtg
     2881 cggctcggtg gtgacgtgga cgtcgaggcg ctcggggcgg cgttgcggga cgtgatcgag
     2941 cggcacgagg tgctgcggac ggtcttcccc gtcgcggacg gcgagccgta ccagcaggtc
     3001 gtcgggatgg acgagttgca ctgggagctg tccgtcggcg aagccaggga cgggctcgag
     3061 ggcgcggtca ccgaggcggt gggtcacgcg ttcgacctgt catcggaggt gccgatccga
     3121 gcctggttgt tcgatggcag tgtgctggtg gtcgtcgtcc accacatagc cagcgacggc
     3181 tggtccatgg cgccgctggc gcgtgacttc tcgctggcgt acgcggcccg tcgtgcgggg
     3241 cgggcgccat cgtgggacgc gctgcccgtc cagtacgccg attacgccct gtggcagcgc
     3301 gacctgctcg gtgccgagga cgatccgggc agcctgatct ccacgcaggt ggcctactgg
     3361 cgggaggcct tggcgggggc gccggaggag ctggcgttgc cgttcgaccg gccgcgtccg
     3421 gtcgtggcct ctcaccgggg tcacagcgtg ccgctgctgg tcccggctga ggtgcatgtg
     3481 cggctcgtgg aggtggccag ggctgagggc gtgacggtgt tcatggtgtt gcaggccgcg
     3541 ctggcggtgt tgttgtcgcg gttgggcgcg ggtacggacg tctcgatcgg gtcggcgaat
     3601 gcgggccgtg tggacgtggc gctggaggat ctggtcggct gcttcgtcaa tacgctggtc
     3661 gtgcgggcgg acctgtccgg tgatccggag ttccgcgcgc tgctggggcg ggtgagggag
     3721 cggagcctgg cggcgttcgc tcaccaggac gtgccgttcg agcggttggt ggaggagctg
     3781 gcgccgtcgc ggtcgatggc ccggcacccg ctgttccagg tggtgctgac caagcaggac
     3841 accatcgccg ccgaactgga tctgccgggc gtgcggacgg ggccggctcc cgtggccggg
     3901 accgggcgga cggcggccaa gttcgacctt gacgtgatgg tcagcgaggt gttcgacggc
     3961 gagggggtgc cggctggggt gcggggttcg gtgacggtcg ccaccgatct gttcgaccct
     4021 gagtgggccg agcggatcgc gcggtactgg gtgcgggtgc tggaggtcgt cggcactgac
     4081 acgcggacgc ggctgagtga ggtcgacctc cttgaggagc atgagcggcg ccgggtggtg
     4141 gaggagtgga actccacggc ggccgaaaag acgtctgcgc cggtggtgga gttgttcgag
     4201 ggccaggtgg cgcgtcggcc ggaggcggtg gcgctcgtcg gtcctgaact ggtcacttac
     4261 gccgagttgg atgggcgggc gaatcggctg gcccacgttt tgatcggtga gggggtgggt
     4321 ggcgagtcgg tggtgggggt gtgtttggac cggggggtgg agctgatcgt ggcggtgctg
     4381 gcggtgtgga aggccggggc ggcgtatgtg ccgatcgatc cgcgtcagcc ggtggagcgg
     4441 atcgcctaca tgctcggtga cagccgcgcg gtgttga
//
