LOCUS       NZ_JAHFZZ010000054     11430 bp    DNA     linear   CON 13-SEP-2024
DEFINITION  Nonomuraea sp. NEAU-A123 Scaffold54, whole genome shotgun sequence.
ACCESSION   NZ_JAHFZZ010000054
VERSION     NZ_JAHFZZ010000054.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nonomuraea sp. NEAU-A123
  ORGANISM  Nonomuraea sp. NEAU-A123
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Nonomuraea.
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Zhang,L. and Zhao,J.
  TITLE     Nonomuraea triticisoli sp. nov., a novel actinobacterium isolated
            from rhizospheric soil of wheat in Langfang, Hebei Province, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11430)
  AUTHORS   Zhang,L. and Zhao,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2021) College of Life Sciences, Northeast
            Agricultural University, Changjiang Road No. 600, Xiangfang
            District, Harbin, Heilongjiang Province 150036, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018603905.1-RS_2024_09_13
            Annotation Date                   :: 09/13/2024 04:26:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11,983
            CDSs (total)                      :: 11,901
            Genes (coding)                    :: 11,761
            CDSs (with protein)               :: 11,761
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 28 of 140
            Pseudo Genes (incomplete)         :: 121 of 140
            Pseudo Genes (internal stop)      :: 3 of 140
            Pseudo Genes (multiple problems)  :: 12 of 140
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:52:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 11430
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHFZZ010000054.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..1133
                     /locus_tag="KLK06_RS59075"
                     /old_locus_tag="KLK06_59080"
     source          1..11430
                     /collection_date="2016-05-10"
                     /db_xref="taxon:2839649"
                     /geo_loc_name="China: Langfang"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Nonomuraea sp. NEAU-A123"
                     /strain="NEAU-A123"
                     /submitter_seqid="Scaffold54"
     region          1..11430
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX) and not alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..11430
                     /SMILES="NC(CC(=O)N)C(=O)NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /kind="neighbouring"
                     /product="NRPS"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    1..11430
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1515:>11430](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1516..>11430
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     protocluster    1..11430
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<4:1133](+)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      <5..1133
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="2"
     CDS             <5..1133
                     /NRPS_PKS="Domain: PCP (294-362). E-value: 8.9e-30. Score:
                     94.7. Matches aSDomain: nrpspksdomains_KLK06_RS59075_PCP.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012711.5"
                     /locus_tag="KLK06_RS59075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_59080"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_214644693.1"
                     /sec_met_domain="AMP-binding (E-value: 2.4e-53, bitscore:
                     169.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.5e-20, bitscore:
                     62.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GELHIVAEEAATDPEALRALLAEKKIDFVKMVPSHLAAMGPGLLP
                     ARSLVLGGEAAAPDWLEQVLTTADERGCAVFNHYGPTETTIGVATARLRGGLTPVANTS
                     LYVLDEHLAPVPPGVSGELYVAGAQLARGYLGRPGLSAERFVACPFVPGARMYRTGDRF
                     RWSTDGGLVFGGRVDDQVKIRGFRVEPGEVGAVLTGCPGVEQIAVVAREEAPGDVRLVG
                     YLVAQDADNAGDLPSVVRRFAADRLPDHMVPSAWVVLDALPLTSNGKLDRHALPAPDHA
                     AAAGAGRGPSDAREEALCAAFAEVLGIPAVGVDDDFFELGGHSLLAVRLVSRIRALLGV
                     DVQVRTLFDAPTVATLARQVGEEKTTRPALRPMRDR"
     CDS_motif       351..440
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59075_0001"
                     /evalue="1.00E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="KLK06_RS59075"
                     /protein_end="145"
                     /protein_start="115"
                     /score="53.0"
                     /tool="antismash"
                     /translation="PPGVSGELYVAGAQLARGYLGRPGLSAERF"
     misc_feature    401..403
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS_motif       528..584
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59075_0002"
                     /evalue="4.70E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="KLK06_RS59075"
                     /protein_end="193"
                     /protein_start="174"
                     /score="34.7"
                     /tool="antismash"
                     /translation="GRVDDQVKIRGFRVEPGEV"
     aSDomain        888..1091
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59075_PCP.1"
                     /evalue="8.90E-30"
                     /label="KLK06_RS59075_PCP.1"
                     /locus_tag="KLK06_RS59075"
                     /protein_end="362"
                     /protein_start="294"
                     /score="94.7"
                     /tool="antismash"
                     /translation="EEALCAAFAEVLGIPAVGVDDDFFELGGHSLLAVRLVSRIRALLG
                     VDVQVRTLFDAPTVATLARQVGE"
     CDS_motif       894..926
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59075_0003"
                     /evalue="9.00E-04"
                     /label="Cy4"
                     /locus_tag="KLK06_RS59075"
                     /protein_end="307"
                     /protein_start="296"
                     /score="12.3"
                     /tool="antismash"
                     /translation="ALCAAFAEVLG"
     misc_feature    complement(1427..1440)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.36"
                     /tool="antismash"
     gene            1516..4629
                     /locus_tag="KLK06_RS59080"
                     /old_locus_tag="KLK06_59085"
     CDS             1516..4629
                     /NRPS_PKS="Domain: Condensation_DCL (14-307). E-value:
                     4.1e-48. Score: 156.0. Matches aSDomain:
                     nrpspksdomains_KLK06_RS59080_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (452-852). E-value:
                     1.5e-100. Score: 328.6. Matches aSDomain:
                     nrpspksdomains_KLK06_RS59080_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (960-1029). E-value: 1.5e-19. Score:
                     61.9. Matches aSDomain: nrpspksdomains_KLK06_RS59080_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /locus_tag="KLK06_RS59080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_59085"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_214644694.1"
                     /sec_met_domain="Condensation (E-value: 2.8e-38, bitscore:
                     120.0, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 8e-109, bitscore:
                     352.3, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.1e-13, bitscore:
                     39.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRSGDRAKLPPGLSDAYPMSALQLGIVYHTLSGEGETPYLNSCGS
                     RVRGLGLDLEVFDRAVQNVVNRHAVLRTSFDLTTYGEAMQLVHAAATVRREVHDLRDLP
                     EQERERRLRADRRRPFDLAEAPLLRCVLHRLAEDEFHCTVVYHQAILDRDSLLRVLDET
                     LSRYADLLADPGAPAAAPPSEVFAEYVAAERAAIDDAEHAKRWQDHLSGYAPKPLSVGA
                     GGATAYGSIESPVPDGIGDALRELSGEAGVPVEDVLLAAHLKVLSLLTGDGDVATGVAM
                     RPPHGSHESHVAAGVFLNVLPLRVDVPGGHWLDLVRRVSGQVKAMLPYSGYPLARVVSD
                     VGGADLFDSMFEFSDTPFPITHRIEPSNLALSTRFLRDSRTGRLSLALDYRRLDDEQAM
                     VIRDYYFATLRAMAVPNGRHDAFSPLSAAERKRMLVDWNGPVRDYARGCLHELFERQVS
                     RTPDALAVLSDEVELTYAELNARANRLARELRDLGAGPESVVGVFAERDADMVVTLLGV
                     LKSGAAYLPLDPGYPEERLRYLLDDARVRLVVTRSHLAPRLPEGNWRVLCTDTRAADIG
                     RHPADDLTPLSTPANLAYVIYTSGSTGRPKGVQVPHSGLVNYLNWCVEGYASRGHGGAP
                     VFSSFSFDMIVPNLYTPLIMGERVCMLPEELPVHELGSRLERLAPFTFIKMTPGHLDLL
                     SEVLGRAAARNLAGTLVVGADAFPSRNLANWRRLDPDTVVLNEYGPTEASVGNTIFHTQ
                     RPVERDLLPIGRAIPNTTMYVLDPAMNPVPVGVTGELFIGGECVVRGYAGRPALTASRF
                     LPDPFSERVGARMYRTGDLGRWLPDGNLEFLGRVDDQVKLNGYRVEPGEVENVLATHPA
                     VARSVAAILGEGSQRRLVGYYVPQAEVSEEELMAYLAERVPGYLLPSALHAIEAVPLNA
                     NGKVDRKALPDTRERSGPAAATFTSPRTPMEVALAGIWEDLLGVAPIGVEDDFAALGGD
                     SILATRMAFRIREDVSLDVAMADIRGSRDLAELAGRLTSSAANRPADR"
     aSDomain        1558..2436
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59080_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="4.10E-48"
                     /label="KLK06_RS59080_Condensation_DCL.1"
                     /locus_tag="KLK06_RS59080"
                     /protein_end="307"
                     /protein_start="14"
                     /score="156.0"
                     /specificity="C activity: inactive"
                     /specificity="Active site motif: HQAILDR"
                     /tool="antismash"
                     /translation="DAYPMSALQLGIVYHTLSGEGETPYLNSCGSRVRGLGLDLEVFDR
                     AVQNVVNRHAVLRTSFDLTTYGEAMQLVHAAATVRREVHDLRDLPEQERERRLRADRRR
                     PFDLAEAPLLRCVLHRLAEDEFHCTVVYHQAILDRDSLLRVLDETLSRYADLLADPGAP
                     AAAPPSEVFAEYVAAERAAIDDAEHAKRWQDHLSGYAPKPLSVGAGGATAYGSIESPVP
                     DGIGDALRELSGEAGVPVEDVLLAAHLKVLSLLTGDGDVATGVAMRPPHGSHESHVAAG
                     VFLNVLPLRVDV"
     aSModule        1558..4602
                     /complete
                     /domains="nrpspksdomains_KLK06_RS59080_Condensation_DCL.1"
                     /domains="nrpspksdomains_KLK06_RS59080_AMP-binding.1"
                     /domains="nrpspksdomains_KLK06_RS59080_PCP.1"
                     /locus_tags="KLK06_RS59080"
                     /monomer_pairings="Asn -> Asn"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       1669..1740
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59080_0001"
                     /evalue="2.40E-08"
                     /label="C2_LCL_024-062"
                     /locus_tag="KLK06_RS59080"
                     /protein_end="75"
                     /protein_start="51"
                     /score="26.4"
                     /tool="antismash"
                     /translation="LDLEVFDRAVQNVVNRHAVLRTSF"
     aSDomain        2872..4071
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59080_AMP-binding.1"
                     /evalue="1.50E-100"
                     /label="KLK06_RS59080_AMP-binding.1"
                     /locus_tag="KLK06_RS59080"
                     /protein_end="852"
                     /protein_start="452"
                     /score="328.6"
                     /specificity="substrate consensus: Asn"
                     /tool="antismash"
                     /translation="FERQVSRTPDALAVLSDEVELTYAELNARANRLARELRDLGAGPE
                     SVVGVFAERDADMVVTLLGVLKSGAAYLPLDPGYPEERLRYLLDDARVRLVVTRSHLAP
                     RLPEGNWRVLCTDTRAADIGRHPADDLTPLSTPANLAYVIYTSGSTGRPKGVQVPHSGL
                     VNYLNWCVEGYASRGHGGAPVFSSFSFDMIVPNLYTPLIMGERVCMLPEELPVHELGSR
                     LERLAPFTFIKMTPGHLDLLSEVLGRAAARNLAGTLVVGADAFPSRNLANWRRLDPDTV
                     VLNEYGPTEASVGNTIFHTQRPVERDLLPIGRAIPNTTMYVLDPAMNPVPVGVTGELFI
                     GGECVVRGYAGRPALTASRFLPDPFSERVGARMYRTGDLGRWLPDGNLEFLGRVDDQVK
                     L"
     CDS_motif       3058..3099
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59080_0002"
                     /evalue="2.60E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="KLK06_RS59080"
                     /protein_end="528"
                     /protein_start="514"
                     /score="17.4"
                     /tool="antismash"
                     /translation="LGVLKSGAAYLPLD"
     CDS_motif       3292..3351
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59080_0003"
                     /evalue="1.50E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="KLK06_RS59080"
                     /protein_end="612"
                     /protein_start="592"
                     /score="32.8"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVQVPH"
     CDS_motif       3862..3951
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59080_0004"
                     /evalue="2.10E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="KLK06_RS59080"
                     /protein_end="812"
                     /protein_start="782"
                     /score="45.6"
                     /tool="antismash"
                     /translation="PVGVTGELFIGGECVVRGYAGRPALTASRF"
     CDS_motif       4039..4104
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59080_0005"
                     /evalue="1.60E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="KLK06_RS59080"
                     /protein_end="863"
                     /protein_start="841"
                     /score="36.1"
                     /tool="antismash"
                     /translation="FLGRVDDQVKLNGYRVEPGEVE"
     aSDomain        4396..4602
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59080_PCP.1"
                     /evalue="1.50E-19"
                     /label="KLK06_RS59080_PCP.1"
                     /locus_tag="KLK06_RS59080"
                     /protein_end="1029"
                     /protein_start="960"
                     /score="61.9"
                     /tool="antismash"
                     /translation="EVALAGIWEDLLGVAPIGVEDDFAALGGDSILATRMAFRIREDVS
                     LDVAMADIRGSRDLAELAGRLTSS"
     gene            4652..5923
                     /locus_tag="KLK06_RS59085"
                     /old_locus_tag="KLK06_59090"
     CDS             4652..5923
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1244: amidohydrolase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /locus_tag="KLK06_RS59085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_59090"
                     /product="Zn-dependent hydrolase"
                     /protein_id="WP_214644695.1"
                     /transl_table=11
                     /translation="MKTRSAQALRVAGDRLVANIRTLGAIGATADGGVTRLAFTAEDAR
                     GRELVRDLMTEAGLRTSVDSAANIIGHRAGERPDLPALMLGSHIDTVPNGGALDGAYGV
                     LAAIEVARALNEHGTPLAHPLAVVAFNNEEGAFGTRGMWGAHALVGALDEADLTAVDDR
                     GRLVADSLADLGGDAAHIDRAVWRPNQMAAYLELHIEQGPVLESRDVRIGVVEAITGRS
                     TLTISVRGKASHAGTTPMELRQDALVAAAHVILAVGGLAYPNGPVRVATVGALSLAPNA
                     WNVVPGTVTLQVDLRDVSDAATRQAIARLRGELLDIAMRTATTIEVTDQHGVTAMSCDA
                     GLRDVIAEASESLDLSHLSLPSGAGHDAQIIGRIAPIGMIFVPSKGGVSHAPEEYSADD
                     DLIAGANVLLQSVLTYDERYGRAF"
     gene            5955..6575
                     /locus_tag="KLK06_RS59090"
                     /old_locus_tag="KLK06_59095"
     CDS             5955..6575
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lysine_decarbox"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /locus_tag="KLK06_RS59090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_59095"
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="WP_214644696.1"
                     /sec_met_domain="Lysine_decarbox (E-value: 3.5e-43,
                     bitscore: 135.4, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLNGPSRSKAIAVFCGARAGVRAHYLHVAREFGAALARRGAGLVY
                     GAAGVGIMGAVAGAVTTAGGTVTGVIPHHLYEREKPELASGNIYVVGSMHERKALMYDL
                     SMGFAVLPGGLGTLDELMEVATWNQLQIHEKPIVLLNQAGFFDPLITMLDHVVAEGFLT
                     QEERAAIRVTADIDDALDWLGLPVTPAPLEESLAGLEPAQPVV"
     misc_feature    6615..6631
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.88"
                     /tool="antismash"
     misc_feature    complement(6629..6644)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.27"
                     /tool="antismash"
     gene            6798..>11430
                     /locus_tag="KLK06_RS59095"
                     /old_locus_tag="KLK06_59100"
     CDS             6798..>11430
                     /NRPS_PKS="Domain: Condensation_LCL (1-302). E-value:
                     1.9e-114. Score: 373.6. Matches aSDomain:
                     nrpspksdomains_KLK06_RS59095_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (474-859). E-value:
                     2.4e-104. Score: 341.1. Matches aSDomain:
                     nrpspksdomains_KLK06_RS59095_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (965-1027). E-value: 1.3e-26. Score:
                     84.6. Matches aSDomain: nrpspksdomains_KLK06_RS59095_PCP.1"
                     /NRPS_PKS="Domain: Condensation_Dual (1079-1369). E-value:
                     7.7e-109. Score: 355.5. Matches aSDomain:
                     nrpspksdomains_KLK06_RS59095_Condensation_Dual.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="KLK06_RS59095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_59100"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_214644697.1"
                     /sec_met_domain="Condensation (E-value: 9.4e-71, bitscore:
                     226.7, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 7.5e-111, bitscore:
                     358.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.1e-17, bitscore:
                     52.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIPLSFAQRRLWFIERLDGPSATYNIPVALRLAGRVDKRALATAL
                     RDVLERHEVLRTVIGVADGEPFQRVLGMDGLDWRLEEVELPPSADLAAAVGEVWEHTFD
                     LAAEVPIRACLFSAGPDDHVLVVVLHHIAGDGWSKGPLARDVSEAYAARREGRAPGWEP
                     LPVQYADYTLWQRELLGDENDPASLLARQLAYWRETLAGAPEELALPFDRPRPPVATHR
                     GHSAPFELPPDAHARLIKVARREGVTTYMVMQAALAMLLSRLGAGTDIPIGQVVAGRTD
                     VALEDLVGFFVNTLVLRTDVSGDPGFREVLARVRETSLSAFSHQDVPFEKLVEELTPSR
                     SLARHPLFQVLLTVQNVAQAAVDLPGVRAGGDVPGSGGGAVSRFDVEVSAGELFDADGA
                     PAGLRGSVIVAADVFTAESAGLFAERLRRVLELVIDEPETRLSETGVLSAQERHKLLVE
                     WNDTAGEIPSGTVPELFAAQAARTPGATAVVYEDTRITYADLDARANRLAHHLNTLGVG
                     PESIVGLCLPRGADIVTAILAVWKAGAAYVPLDPAHPADRLAFAVADSGVRVLVTDRDE
                     TLGAAHVVRLDDPCLGTYPDTPRAERPLAGELAYVIYTSGSTGRPKGVAVEHASLANLV
                     SVFGPLMEVGPGVPVLQFASFTFDASVLDVAVTLARGGTLVVASTTERAEPALLRALVE
                     TTGVRSASVVPSLLAVLETGDLSGVEAMVVGSEHMPPGLARDWARERRLVHAYGPTEAT
                     VITAAGRVDPGEGGALPFGGPLANTRMFVLDDFLEPVAPGVAGELYIAGAQVARGYVGR
                     SALTAERFVASPFATGERLYRTGDLVRWTGDGRLVFAGRADEQVKVRGFRIEPGEVQAA
                     VAAHPSVARAAVVAREDTPGETRLVAYVVSADDAPGLPESVRAFTADRLPEYMVPSAVV
                     VLDDLPLTPNGKLDRRALPAPAYETGLGRRPSNPREEIVCAAFADVLGLPEVGVDDDFF
                     TLGGHSLLAIRLVERLREQGVEVSVRALFQSPTPAGLAWSSGAMPVTVPDNLIPAGTTR
                     ITPEMLPLADLTSDEIERVVATVEGGAANVADVYPLAPLQEGLLFHHLLAGGGDDAYAL
                     PTVAEFDTRDRLDAFLDALQRVVDRHDILRTGIVWDGLREPVQVVWRRAALPVEEVTLD
                     PAGTDPVADLVTAGGSSMDLGRAPLIRVHTAARPGGDGRWLALLRIHHMVQDHTAKEVL
                     LDEVQAFLAGRGGTLPEPLPFRTFVAQARGGTGEHAGYFAELLGDVHEPTAPYGVMNAR
                     GDGAGVVREELDLSPELTGRLRAAALRMGVSPAPVMHVAWARALAAISGRADVVFGTVL
                     FGRMNAGMGSDRVPGPFMNTLPVRVRTDEPGVLAAVSAMRDQLGELLEHEHASLAVAQR
                     ASGVTGDTPLFTSFFNYRRNTGRGGGGLEGIRTVFTHDRTNYPLAVAVNDDGDALSLTI
                     DAVAPIHPRAVARLVRTATAGLVSALETALDGGSELPLGDVDVLDEVERRLVLEGWNAT
                     AVEVGGVSVVELFEGQVARRPDAV"
     aSDomain        6801..7703
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59095_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.90E-114"
                     /label="KLK06_RS59095_Condensation_LCL.1"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="302"
                     /protein_start="1"
                     /score="373.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIAGDG"
                     /tool="antismash"
                     /translation="IPLSFAQRRLWFIERLDGPSATYNIPVALRLAGRVDKRALATALR
                     DVLERHEVLRTVIGVADGEPFQRVLGMDGLDWRLEEVELPPSADLAAAVGEVWEHTFDL
                     AAEVPIRACLFSAGPDDHVLVVVLHHIAGDGWSKGPLARDVSEAYAARREGRAPGWEPL
                     PVQYADYTLWQRELLGDENDPASLLARQLAYWRETLAGAPEELALPFDRPRPPVATHRG
                     HSAPFELPPDAHARLIKVARREGVTTYMVMQAALAMLLSRLGAGTDIPIGQVVAGRTDV
                     ALEDLVGFFVNTLVLRTDVS"
     aSModule        6801..9878
                     /complete
                     /domains="nrpspksdomains_KLK06_RS59095_Condensation_LCL.1"
                     /domains="nrpspksdomains_KLK06_RS59095_AMP-binding.1"
                     /domains="nrpspksdomains_KLK06_RS59095_PCP.1"
                     /locus_tags="KLK06_RS59095"
                     /monomer_pairings="X -> X"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       6861..6974
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0001"
                     /evalue="4.70E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="59"
                     /protein_start="21"
                     /score="51.2"
                     /tool="antismash"
                     /translation="ATYNIPVALRLAGRVDKRALATALRDVLERHEVLRTVI"
     CDS_motif       7182..7247
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0002"
                     /evalue="9.90E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="150"
                     /protein_start="128"
                     /score="27.7"
                     /tool="antismash"
                     /translation="LHHIAGDGWSKGPLARDVSEAY"
     CDS_motif       7296..7334
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0003"
                     /evalue="1.80E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="179"
                     /protein_start="166"
                     /score="17.2"
                     /tool="antismash"
                     /translation="QYADYTLWQRELL"
     CDS_motif       7602..7691
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0004"
                     /evalue="4.30E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="298"
                     /protein_start="268"
                     /score="41.6"
                     /tool="antismash"
                     /translation="DIPIGQVVAGRTDVALEDLVGFFVNTLVLR"
     CDS_motif       7716..7742
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0005"
                     /evalue="3.70E+01"
                     /label="C4_dual_169-182"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="315"
                     /protein_start="306"
                     /score="-1.8"
                     /tool="antismash"
                     /translation="FREVLARVR"
     CDS_motif       7740..7856
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0006"
                     /evalue="4.00E-21"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="353"
                     /protein_start="314"
                     /score="67.5"
                     /tool="antismash"
                     /translation="RETSLSAFSHQDVPFEKLVEELTPSRSLARHPLFQVLLT"
     aSDomain        8220..9374
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59095_AMP-binding.1"
                     /evalue="2.40E-104"
                     /label="KLK06_RS59095_AMP-binding.1"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="859"
                     /protein_start="474"
                     /score="341.1"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FAAQAARTPGATAVVYEDTRITYADLDARANRLAHHLNTLGVGPE
                     SIVGLCLPRGADIVTAILAVWKAGAAYVPLDPAHPADRLAFAVADSGVRVLVTDRDETL
                     GAAHVVRLDDPCLGTYPDTPRAERPLAGELAYVIYTSGSTGRPKGVAVEHASLANLVSV
                     FGPLMEVGPGVPVLQFASFTFDASVLDVAVTLARGGTLVVASTTERAEPALLRALVETT
                     GVRSASVVPSLLAVLETGDLSGVEAMVVGSEHMPPGLARDWARERRLVHAYGPTEATVI
                     TAAGRVDPGEGGALPFGGPLANTRMFVLDDFLEPVAPGVAGELYIAGAQVARGYVGRSA
                     LTAERFVASPFATGERLYRTGDLVRWTGDGRLVFAGRADEQVKVR"
     CDS_motif       8406..8447
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0007"
                     /evalue="1.90E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="550"
                     /protein_start="536"
                     /score="20.8"
                     /tool="antismash"
                     /translation="LAVWKAGAAYVPLD"
     CDS_motif       8622..8681
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0008"
                     /evalue="5.50E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="628"
                     /protein_start="608"
                     /score="34.2"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVAVEH"
     CDS_motif       9171..9257
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0009"
                     /evalue="1.90E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="820"
                     /protein_start="791"
                     /score="48.9"
                     /tool="antismash"
                     /translation="PGVAGELYIAGAQVARGYVGRSALTAERF"
     CDS_motif       9339..9401
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0010"
                     /evalue="9.60E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="868"
                     /protein_start="847"
                     /score="33.7"
                     /tool="antismash"
                     /translation="FAGRADEQVKVRGFRIEPGEV"
     aSDomain        9693..9878
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59095_PCP.1"
                     /evalue="1.30E-26"
                     /label="KLK06_RS59095_PCP.1"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="1027"
                     /protein_start="965"
                     /score="84.6"
                     /tool="antismash"
                     /translation="EEIVCAAFADVLGLPEVGVDDDFFTLGGHSLLAIRLVERLREQGV
                     EVSVRALFQSPTPAGLA"
     aSDomain        10035..10904
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLK06_RS59095_Condensation_Dual.
                     1"
                     /domain_subtypes="Condensation_Dual"
                     /evalue="7.70E-109"
                     /label="KLK06_RS59095_Condensation_Dual.1"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="1369"
                     /protein_start="1079"
                     /score="355.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHMVQDH"
                     /tool="antismash"
                     /translation="DVYPLAPLQEGLLFHHLLAGGGDDAYALPTVAEFDTRDRLDAFLD
                     ALQRVVDRHDILRTGIVWDGLREPVQVVWRRAALPVEEVTLDPAGTDPVADLVTAGGSS
                     MDLGRAPLIRVHTAARPGGDGRWLALLRIHHMVQDHTAKEVLLDEVQAFLAGRGGTLPE
                     PLPFRTFVAQARGGTGEHAGYFAELLGDVHEPTAPYGVMNARGDGAGVVREELDLSPEL
                     TGRLRAAALRMGVSPAPVMHVAWARALAAISGRADVVFGTVLFGRMNAGMGSDRVPGPF
                     MNTLPVRVR"
     CDS_motif       10041..10082
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0011"
                     /evalue="9.00E-07"
                     /label="C1_dual_004-017"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="1095"
                     /protein_start="1081"
                     /score="21.2"
                     /tool="antismash"
                     /translation="YPLAPLQEGLLFHH"
     CDS_motif       10131..10223
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0012"
                     /evalue="2.60E-15"
                     /label="C2_dual_024-063"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="1142"
                     /protein_start="1111"
                     /score="49.0"
                     /tool="antismash"
                     /translation="EFDTRDRLDAFLDALQRVVDRHDILRTGIVW"
     CDS_motif       10434..10496
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0013"
                     /evalue="9.60E-06"
                     /label="C3_dual_135-156"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="1233"
                     /protein_start="1212"
                     /score="18.6"
                     /tool="antismash"
                     /translation="HHMVQDHTAKEVLLDEVQAFL"
     CDS_motif       10530..10565
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0014"
                     /evalue="6.40E-03"
                     /label="C4_dual_169-182"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="1256"
                     /protein_start="1244"
                     /score="9.6"
                     /tool="antismash"
                     /translation="PFRTFVAQARGG"
     CDS_motif       10803..10898
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0015"
                     /evalue="1.70E-12"
                     /label="C5_dual_260-291"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="1367"
                     /protein_start="1335"
                     /score="39.7"
                     /tool="antismash"
                     /translation="DVVFGTVLFGRMNAGMGSDRVPGPFMNTLPVR"
     CDS_motif       10950..11042
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_KLK06_RS59095_0016"
                     /evalue="3.20E-07"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="KLK06_RS59095"
                     /protein_end="1415"
                     /protein_start="1384"
                     /score="22.7"
                     /tool="antismash"
                     /translation="QLGELLEHEHASLAVAQRASGVTGDTPLFTS"
ORIGIN
        1 gtggtgagtt gcacattgtg gcggaggagg cggccaccga tccggaggcc ctgcgcgcgt
       61 tgctggccga gaagaagatc gactttgtca agatggtgcc gtcgcacttg gcggcgatgg
      121 gtccggggtt gttgcctgcg cggtcgctgg tgctgggtgg tgaggcggcg gcccctgact
      181 ggctggagca ggtcctcacg accgccgatg agcgcgggtg tgcggtgttc aatcactatg
      241 gtccgaccga gaccacgatc ggcgtggcca ccgcccggtt gcgcggcggg ctgaccccgg
      301 tggccaacac cagtctgtat gtgctggatg agcacctggc gccggtgccg ccgggtgtga
      361 gcggtgagct gtatgtggcc ggtgctcagc tggcgcgtgg ttatctgggt cgtcctgggt
      421 tgagcgctga gcggtttgtg gcgtgcccgt tcgtgcccgg tgcgcgcatg tatcgcaccg
      481 gtgaccggtt ccgctggagc actgatggcg ggttggtgtt cggcggccgg gtggacgacc
      541 aggtcaagat ccgcggcttc cgcgtagaac cgggtgaggt gggcgcggtg ctcaccggct
      601 gtcctggggt ggagcagatc gccgtggtgg cccgggagga agcacccggc gacgtccgtc
      661 tcgtcggcta tctggtggcg caggacgcgg acaacgccgg tgatctgccg tcggtggtgc
      721 gcagattcgc cgccgatcgg ttgcctgatc acatggtgcc gtcggcctgg gtggtgctgg
      781 acgcgctgcc gctgacgagt aacggcaagc tcgaccgcca cgcactgccc gcccccgacc
      841 acgccgccgc ggccggtgcc gggcgcggcc cgtccgacgc gcgggaggag gccctatgcg
      901 cggcgttcgc cgaggtgttg gggatcccgg cggtcggcgt cgatgatgac ttcttcgagc
      961 tgggcggcca ctccctgctc gccgtccgcc tggtcagccg catccgcgcc ctcctgggcg
     1021 tggacgtcca ggtccgtacg ctcttcgacg cccccacggt cgcgaccctc gcgcggcagg
     1081 tgggggagga gaagaccacc aggccggccc tgcgccccat gcgcgaccgc tgaccaccaa
     1141 cgcgtgcgaa ggcgttcaac tgcggcgtca gcccagttga acgccttcgc ggcacatgca
     1201 tggcattgtt cgtgagccgc ggcatacgtc atagtggacc ccggcgaaac ggcaatgccc
     1261 gggccggtga ttcgcgcttt cgccgcctca cgactttctt atcgatttgc tcggttccgc
     1321 ttcagcggct tttccgcccc gcgggcgaat ctcgggtgtc gcgctccgat cgcaggagct
     1381 gtcgccggag gaacggcgag aaattctacg tagctgtaga aggcgagtat aagaatgaga
     1441 gacacgtcca cggagatcag gccggtaggc agccggaaaa gtgccggcac agccgcactc
     1501 gaaccgttct cgctcgtgag aagcggggac cgggccaaac tgccgcccgg gctttccgat
     1561 gcctatccca tgtccgcgct gcaactgggc atcgtctacc acacgctttc cggagaaggg
     1621 gagacacctt atctgaactc ctgcggttcg cgcgtgcgcg ggctcgggct cgacctggag
     1681 gtgttcgatc gggcggtcca gaacgtggtg aaccggcatg ccgtcttgcg gacctccttc
     1741 gacctgacca cgtacggtga ggcgatgcag ctcgtgcacg cggcggcgac cgtgcgacgc
     1801 gaggtgcacg atctgcgtga cctgcccgag caggagcggg agcgtcggct gcgcgccgac
     1861 aggcgccggc cgttcgatct cgccgaggcg ccgctcctgc gctgcgtgct gcaccgcctg
     1921 gcggaggacg agttccactg caccgtggtg taccaccagg cgattctcga cagggacagc
     1981 ctgctccgcg tgctcgacga gacgctgagc cggtacgcgg acctgctcgc cgatccgggc
     2041 gccccggccg ccgcgccacc ctctgaggtg ttcgcggagt acgtcgcggc ggagcgggcg
     2101 gccatcgacg acgcggagca cgccaagcgc tggcaggacc acctctccgg ctacgcgccg
     2161 aagcccctgt cggtgggcgc gggtggcgcc acggcgtacg ggtcgatcga gtcgcccgtc
     2221 ccggacggca tcggcgacgc gctgcgcgag ctgagcggcg aggcgggcgt gccggtcgag
     2281 gacgtgctgc tggcggcgca cctgaaagtg ctgagcctgc tgaccggcga cggcgacgtc
     2341 gccaccgggg tggccatgcg gccaccccat gggtcccatg agtcacacgt ggccgcgggt
     2401 gtcttcctga acgtcctgcc gctccgggtg gacgtgccgg gcgggcattg gctggatctg
     2461 gtgcgccgcg tgtccggtca ggtgaaggcg atgctcccgt acagcggata cccgctggcc
     2521 cgggtcgtgt cggatgtggg cggcgccgat ctgttcgact ccatgttcga attcagtgac
     2581 acgccttttc cgatcaccca tcggatcgag cccagcaacc tcgccctctc gacgcggttc
     2641 ctccgggatt cccggaccgg gcggttgtcc ctggcgctgg actaccggcg gctggacgac
     2701 gagcaggcga tggtgatccg cgactactac ttcgcgacgt tgagggcgat ggcggtgccg
     2761 aacggccgtc atgacgcgtt ctcgccgctg agcgccgcgg agcggaagcg catgctggtg
     2821 gactggaacg gcccggtacg cgactacgcg cggggctgcc tgcacgagct cttcgagcgg
     2881 caggtgagcc ggaccccgga cgcgctcgcg gtgctgagcg acgaggtcga gctcacttat
     2941 gccgagctca acgccagggc gaaccggctc gcgagggagt tgcgcgacct cggggccggg
     3001 ccggagtccg tggtcggcgt gttcgccgag cgggacgcgg acatggtcgt caccttgctc
     3061 ggcgtgctca agtccggcgc cgcctacctg ccgctggacc cgggctaccc ggaggaacgg
     3121 ctgcgctacc tgctcgacga cgcccgggtc cgcctcgtcg tcaccagatc tcacctcgcg
     3181 ccccggttgc cggaggggaa ctggcgggtc ctgtgcaccg acacgcgggc cgcggacatc
     3241 ggccgccacc ccgctgacga tctcaccccg ctgagcacac ccgccaacct ggcgtatgtc
     3301 atctacacct ccggctcgac cggccggccg aaaggcgtcc aggtcccgca ttccggcctg
     3361 gtcaactacc tgaactggtg cgtcgagggg tacgcgagcc ggggccatgg tggagcaccg
     3421 gtgttctcct ccttctcctt cgacatgatc gtgcccaacc tctacacccc gctgatcatg
     3481 ggcgagcgcg tctgcatgct gccggaggaa ctgccggtgc acgaactcgg ctcccgcctg
     3541 gaacggctgg ccccgttcac cttcatcaag atgaccccgg gccacctgga cctgctctcc
     3601 gaagtgctcg gacgggccgc ggccaggaac ctggccggca ccctcgtagt cggcgcggac
     3661 gccttcccca gccgcaatct cgccaactgg cggcgactgg accccgacac ggtcgtcctc
     3721 aacgagtacg gcccgacgga ggcttcggtg ggcaacacca tcttccacac ccagcgcccc
     3781 gtcgaacgcg acctactccc catcgggcgc gccatcccga acaccaccat gtatgtgctg
     3841 gacccggcga tgaaccccgt accggtcggc gtcacgggag agctgttcat cggcggggaa
     3901 tgcgtggtgc gcggctacgc cggccgaccg gcgctgacgg cgagccgttt cctgccggac
     3961 cccttcagcg agcgggtcgg cgcccggatg taccgcaccg gggacctggg cagatggctg
     4021 ccggacggca acctcgagtt cctgggcagg gtggacgacc aggtgaagct gaacggctac
     4081 cgggtcgagc cgggcgaggt cgagaacgtg ctggccacgc atccggccgt cgcgcggtcg
     4141 gtggccgcga tcctcggcga gggctcccag cgccggctgg tcggctacta cgtgccccag
     4201 gcggaggtga gcgaggagga gctcatggcc tatctcgccg agcgggtgcc cggctacctg
     4261 ctgccgtcgg cgctgcacgc catcgaggcc gtgccgctca acgcgaacgg aaaggtcgat
     4321 cgcaaggccc tgcccgatac ccgggagcgg agcggaccgg cggcggccac gttcacctcg
     4381 ccccgcaccc ccatggaggt ggcgctggcc ggcatctggg aggacctgct cggagtggcg
     4441 ccgatcggcg tggaggacga cttcgccgcc ctgggcggtg actcgatcct ggccaccagg
     4501 atggcgttca ggatcaggga agacgtcagt ctcgacgttg cgatggcgga catccggggc
     4561 agccgcgacc tggcggagct ggccggacgc ctgacctcct cggcggcgaa ccggccggcg
     4621 gatcggtgag ggcccgacca tgggagacga cgtgaagacg cgttcggccc aggccctgcg
     4681 ggtcgccggc gatcgattgg tggccaacat ccggaccctc ggcgcgatag gcgccacggc
     4741 cgacggcggc gtcaccaggc tcgccttcac cgcggaggac gcccgcgggc gggagctggt
     4801 ccgcgacctc atgaccgaag ccgggctgcg gaccagcgtc gacagcgcgg cgaacatcat
     4861 cgggcaccgg gcgggagaac gcccggacct gccggcgctc atgctcgggt cgcacatcga
     4921 caccgtgccc aacggcggcg ccctggacgg cgcgtacgga gtcctggcgg ccatcgaggt
     4981 cgcccgcgcg ctcaacgagc acggcacccc gctggcccac cccctcgccg tcgtggcctt
     5041 caacaacgag gaaggcgcgt tcggcacccg gggaatgtgg ggggcgcacg cgctggtggg
     5101 cgcgctcgac gaagccgacc tgaccgccgt ggacgatcgc ggcaggctcg tggcggactc
     5161 gctggccgac ctgggaggag acgcggccca catcgaccgg gccgtgtggc gccctaacca
     5221 gatggccgcc tacctcgagc tgcacatcga gcaaggcccg gtgctcgaga gccgcgacgt
     5281 ccggatcggg gtggtggagg ccatcacggg ccgttcgacg ctcaccatct ccgtacgcgg
     5341 caaggcctcg cacgccggga ccacgccgat ggagctgcgc caggacgcgc tcgtggcggc
     5401 ggcgcacgtg atcctcgcgg taggcggcct cgcctacccc aacggcccgg tacgggtcgc
     5461 caccgtcggc gcgctctcgc tggcccccaa cgcctggaac gtcgtccccg gaaccgtcac
     5521 gctgcaggtg gacctgcgcg acgtgtccga cgccgccacc cggcaggcga tcgcgcgtct
     5581 gcggggcgag ctcctggaca tcgccatgag gaccgccacc acgatcgagg tgacggacca
     5641 gcacggcgtg accgccatgt cctgcgacgc gggcctgcgg gacgtcatcg cggaggcgag
     5701 cgagagcctt gacctgagcc acctctccct gcccagcggc gccggccacg acgcgcagat
     5761 catcgggcgc atcgcaccga taggcatgat cttcgtgccc agcaagggtg gcgtcagcca
     5821 cgcgccggag gagtacagcg ccgacgacga cctgatagcg ggcgcgaacg tgctgctcca
     5881 gtccgtactc acctacgacg aaagatacgg ccgggccttc tgattttctt gcgctgattc
     5941 gttgggagca gagtatgttg aacggtcctt cccgaagcaa agccatcgcc gttttctgcg
     6001 gtgcccgcgc gggcgtccgc gcgcattatc tgcacgtggc ccgagagttc ggcgcggcac
     6061 tggcgcgacg cggcgcgggg ctggtctacg gtgccgccgg cgtcggcatc atgggagcgg
     6121 tcgccggcgc cgtcaccacg gccggcggaa ccgtgacagg ggtgatcccg caccacctgt
     6181 acgagcggga gaagccggag ctcgcgagcg gcaacatcta cgtcgtcggg tccatgcacg
     6241 agcgcaaggc gctcatgtac gacctgtcca tgggcttcgc ggtcctgccc ggcgggctcg
     6301 gcaccctgga cgagctgatg gaggtcgcca cctggaacca gttgcagatc cacgagaagc
     6361 ccatcgtcct gctcaaccag gcgggcttct tcgacccgct gatcaccatg ctggaccacg
     6421 tggtcgccga gggcttcctc acgcaggagg aacgtgccgc catccgcgtg acggccgaca
     6481 tcgacgacgc cctcgactgg ctcggcctgc ccgtcacgcc ggccccgctg gaagagagcc
     6541 tggctgggct ggagccggcc cagccggtgg tgtgacggcg agcgttcaac cgcagcctcg
     6601 gcgcagttga acgccgcaca atggcgcggc gaatgcgtgg cattgttcag gcgcggaggc
     6661 gtaggccata gtgggcccgg tgaaacggcg ggacagtgcg gtgcgctttc gccgccccgc
     6721 cattttttat ggcattgttc gagcggcttt tccgccccca agaacgtcac tggacaacgt
     6781 gagcaaaagg ggtcttgatg attccgttgt cattcgcaca gcgccggttg tggttcatcg
     6841 agcggctcga cggcccgagt gcgacctaca acatcccggt ggcgttgcgg ctggcgggcc
     6901 gagtggacaa gcgggcgctg gccaccgcgc tgcgtgacgt tctggagcgg cacgaggtgc
     6961 tgcggacggt gatcggcgtg gccgacgggg agccgttcca gcgcgtgctc ggcatggacg
     7021 gcctcgactg gcggctcgag gaggtcgagc tgccgccgtc ggccgacctg gccgcggcgg
     7081 tgggcgaggt gtgggagcac acgttcgacc tggccgccga ggtgccgatc agggcgtgcc
     7141 tgttctcggc ggggccggac gatcacgtgc tggtggtcgt gctgcaccac atcgccggtg
     7201 acggctggtc gaaggggccg ctggcccgcg acgtctccga ggcgtacgcg gcccgccgcg
     7261 agggccgggc gccgggctgg gaacccctgc ccgtccagta cgccgactac acgctgtggc
     7321 agcgcgagct gctcggcgac gagaacgatc cggccagcct cctggcgcgc cagctcgcct
     7381 actggcggga gaccctggcc ggggcgccgg aggagctggc gttgccgttc gaccgcccgc
     7441 gcccgccggt cgccacgcac cgcgggcaca gcgcgccgtt cgaactgccg ccggacgcgc
     7501 acgcccggct gatcaaggtg gcgcggcgcg agggcgtcac gacgtacatg gtgatgcagg
     7561 cggcgctggc gatgttgctg tcgcggctgg gcgcgggcac cgacatcccg atcggccagg
     7621 tggtggccgg gcgtaccgac gtcgcgctcg aggatctggt cggcttcttc gtcaacacgc
     7681 tggtgctacg gaccgacgtg tcgggcgatc ccggcttccg cgaggtgctc gcgcgcgtgc
     7741 gggagacgag cctgtcggcg ttctcccacc aggacgtgcc cttcgagaag ctggtcgagg
     7801 agctgacgcc gagccggtcg ctggcccgcc acccgctgtt ccaggtgctg ctcaccgtgc
     7861 agaacgtcgc ccaggcggcc gtggacctgc cgggtgtgcg cgccggggga gacgtgcccg
     7921 gcagcggcgg cggcgcggtg tcgaggttcg acgtcgaggt cagcgccggc gagctgttcg
     7981 acgccgacgg cgctccggcc ggactgcgcg gctcggtgat cgtggcggcg gacgtgttca
     8041 ccgccgagtc ggccgggctg ttcgccgagc ggctgcggcg cgtgctcgaa ctggtcatcg
     8101 acgagcccga gacccggctg agcgagacgg gcgtgctcag cgcgcaagag cgccacaagc
     8161 tgctcgtcga gtggaacgac acggcgggcg agatcccgtc cggcacggtg ccagagctgt
     8221 tcgcggccca ggcggcgcgt acgcccggcg cgaccgcggt cgtgtacgag gacacccgga
     8281 tcacctacgc cgacctcgac gcccgggcga accgcctcgc ccaccacctc aacacgctcg
     8341 gtgtcggccc ggagtccatc gtggggctgt gcctgccacg cggcgccgac atcgtgacgg
     8401 cgatcctcgc ggtgtggaag gccggcgccg cctacgtacc gctcgacccc gcccaccccg
     8461 ctgaccgcct ggccttcgcc gtcgccgaca gcggcgtgcg cgtgctggtg accgaccggg
     8521 acgagacgct cggcgccgcc cacgtcgtac gcctcgacga cccctgcctc ggcacctacc
     8581 cggacacgcc gcgcgcggag cggcccctgg ccggcgagct ggcgtacgtc atctacacct
     8641 ccggctcgac cggccggccg aagggtgtgg cggtcgagca cgcatccctc gcgaacctcg
     8701 tctcggtgtt cgggccgctg atggaggtgg gcccgggcgt gccggtcctg cagttcgcgt
     8761 cgttcacgtt cgacgcctcg gtgctggacg tggcggtcac cctggcccgc ggcggcacgc
     8821 tggtcgtggc gagcacgact gaacgcgcgg agccggccct gctgcgcgcg ctcgtcgaga
     8881 cgaccggcgt gcgctcggcc agcgtcgtcc cctcgctgct cgcggtcctg gagacgggcg
     8941 acctgtccgg tgtcgaggcg atggtcgtgg gctcggagca catgccgccc ggcctggccc
     9001 gggactgggc ccgggagcgg cggctcgtgc acgcgtacgg gccgaccgag gccaccgtga
     9061 tcaccgcggc cgggcgcgtg gaccctggcg agggcggcgc gctgccgttc ggcgggccgc
     9121 tcgccaacac ccgcatgttc gtgctggacg acttcctgga gccggtggcg cccggcgtgg
     9181 cgggcgagct ctacatcgcc ggcgcgcagg tcgcccgcgg ttacgtggga cgctccgcgc
     9241 tgaccgccga acggttcgtg gcctcgccgt tcgcgacggg cgagcggctc taccggaccg
     9301 gcgacctggt gcgatggacc ggcgacggcc ggctcgtctt cgccgggcgc gccgacgagc
     9361 aggtcaaggt gcgcgggttc cggatcgagc cgggtgaggt gcaggcggcc gtcgccgccc
     9421 atccgtcggt ggcgcgggcc gcggtggtcg cgcgtgagga tacgccgggc gagacccggc
     9481 tggtcgctta tgtcgtgtcc gcggatgacg cgcccggcct gccggaatcc gtgcgcgcct
     9541 tcacggccga ccggctgccc gagtacatgg tgccgtcggc cgtcgtcgtc ctggacgacc
     9601 tgccgctcac cccgaacggc aagctcgacc gccgtgccct gcccgccccc gcgtacgaga
     9661 ccggcctcgg ccgccgcccc tcgaaccccc gcgaagagat cgtctgcgcc gccttcgccg
     9721 acgtgctggg cctgcccgag gtcggcgtgg acgacgactt cttcacgctg ggcggccact
     9781 ccctgctggc catccgcctg gtggaacggc tgcgcgagca gggcgtcgag gtgtcggtac
     9841 gcgcgctgtt ccagtccccg accccggcgg gcctcgcttg gtcgtcgggc gcgatgcccg
     9901 tgacggtgcc ggacaatctc atccccgccg gaaccacccg gatcacgccc gagatgctgc
     9961 cgctggccga cctgacctcc gacgagatcg aacgcgtcgt cgccaccgtc gagggcggcg
    10021 cggccaacgt cgccgacgtc tacccgctgg ccccgctgca ggagggcctg ctcttccacc
    10081 acctgctggc cggcggcggc gacgacgcgt acgcgctgcc gacggtcgcc gagttcgaca
    10141 cccgcgaccg gctcgacgcc ttcctcgacg ccctgcagcg cgtggtggac cgccacgaca
    10201 tcctgcggac cggcatcgtc tgggacgggc tacgcgaacc ggtacaggtc gtgtggcgcc
    10261 gcgccgccct gcccgtcgag gaggtgacgc tcgacccggc cggtacggac cccgtggccg
    10321 acctcgttac cgccggcgga tcgtcgatgg acctgggccg cgcgccgctg atcagggtgc
    10381 acaccgccgc ccggcccggc ggtgacggcc gatggctggc cctgctccgc atccaccaca
    10441 tggtccagga ccacacggcc aaggaggtcc tgctcgacga ggtccaggcc ttcctggccg
    10501 ggcgcggcgg cacgctgccc gagccgctgc cgttccggac cttcgtggcc caggcccgcg
    10561 gcggcacagg cgagcacgcc ggctacttcg ccgagctgct gggcgacgtc cacgaaccga
    10621 cggccccgta cggggtcatg aacgcccgcg gcgacggcgc cggagtcgtc cgcgaggaac
    10681 tcgacctctc acccgagctg accggccgcc tgcgcgccgc cgcgctccgc atgggcgtca
    10741 gccccgcgcc ggtgatgcac gtggcctggg cccgcgcgct cgccgccatc agcggccgtg
    10801 ccgacgtggt cttcggcacc gtcctgttcg gccgcatgaa cgccggcatg ggttccgatc
    10861 gcgtacccgg cccgttcatg aacaccctcc cggtccgcgt acgcaccgac gagccgggcg
    10921 tcctggccgc cgtgtcggcg atgcgcgacc agctcggcga actgctggag cacgagcacg
    10981 cctccctggc cgtcgcccag cgggccagcg gcgtcaccgg ggacaccccc ctgttcacct
    11041 cgttcttcaa ctaccgccgc aacaccggcc ggggcggagg gggccttgag ggcatccgca
    11101 cggtcttcac ccacgaccgc accaactacc ccctcgccgt ggccgtcaac gacgacggcg
    11161 acgcgctgtc gctgaccatc gacgccgtcg cgcccatcca cccccgcgcg gtggcccgcc
    11221 tggtccgcac cgccacggcc ggcctggtct ccgcgctgga gacggcgctg gacggaggct
    11281 cggagctgcc gctcggcgat gtggatgtgc tcgatgaggt ggagcggcgg ttggtgctgg
    11341 aggggtggaa tgccactgct gtggaggtgg gtggtgtgtc ggtggtggag ttgtttgagg
    11401 ggcaggtggc tcggaggccg gatgcggtgg
//
