LOCUS       NZ_JAHFZZ010000008     10816 bp    DNA     linear   CON 13-SEP-2024
DEFINITION  Nonomuraea sp. NEAU-A123 Scaffold8, whole genome shotgun sequence.
ACCESSION   NZ_JAHFZZ010000008
VERSION     NZ_JAHFZZ010000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nonomuraea sp. NEAU-A123
  ORGANISM  Nonomuraea sp. NEAU-A123
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Nonomuraea.
REFERENCE   1  (bases 1 to 558580)
  AUTHORS   Zhang,L. and Zhao,J.
  TITLE     Nonomuraea triticisoli sp. nov., a novel actinobacterium isolated
            from rhizospheric soil of wheat in Langfang, Hebei Province, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 558580)
  AUTHORS   Zhang,L. and Zhao,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2021) College of Life Sciences, Northeast
            Agricultural University, Changjiang Road No. 600, Xiangfang
            District, Harbin, Heilongjiang Province 150036, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018603905.1-RS_2024_09_13
            Annotation Date                   :: 09/13/2024 04:26:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11,983
            CDSs (total)                      :: 11,901
            Genes (coding)                    :: 11,761
            CDSs (with protein)               :: 11,761
            Genes (RNA)                       :: 82
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 28 of 140
            Pseudo Genes (incomplete)         :: 121 of 140
            Pseudo Genes (internal stop)      :: 3 of 140
            Pseudo Genes (multiple problems)  :: 12 of 140
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:52:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 479666
            Orig. end                         :: 490482
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHFZZ010000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10816
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10816
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10816
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5816](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5816
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(353..367)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.6"
                     /tool="antismash"
     misc_feature    complement(1635..1650)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.27"
                     /tool="antismash"
     gene            complement(1916..2134)
                     /locus_tag="KLK06_RS27375"
                     /old_locus_tag="KLK06_27370"
     CDS             complement(1916..2134)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLK06_RS27375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLK06_27370"
                     /product="hypothetical protein"
                     /protein_id="WP_214638482.1"
                     /transl_table=11
                     /translation="MDESSGRLLALQQVVVEQASRNPPGDEGLAGLPAGTIYHALVYQK
                     ATWTDTPPTLPADGPAPKNGGRNECLG"
     gene            complement(2089..3609)
                     /locus_tag="KLK06_RS27380"
                     /old_locus_tag="KLK06_27375"
     CDS             complement(2089..3609)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF016430.5"
                     /locus_tag="KLK06_RS27380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_27375"
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_214638483.1"
                     /transl_table=11
                     /translation="MLNQEGFAALFDACHPRVYAYAVSRCGRRLSEEVVSETFVVAWRR
                     RDDIPAKAALPWLLGVARNVIRDVHREELRRVALDDALHGWADETGPDVAEEVAERAAV
                     LRALATLSDEDKEVLMLVSWHGLSSVEAAKVVGSSPVGLLRAAASRQAASGGGHGRLDG
                     GTGSARAGIGGGAVKRKNVLDVLAAARPADLDPPGTPRAQVVELGAPVRGRQVRWVGAV
                     VASLAMASVIAVLGVPRLLGHDTVTGQVTGRSAANVLDVAADRASGQQQVPGRYWHTVG
                     LILGEQVIGSPDNRYRIKTRVQTSRWVPRGPEAYMLFTERGMPTVRASAANEQAWRRAG
                     APKLCGVDSDCGNDTAPLGRTRFMFRPSTWPIQDQGLTLSASELLDLPRDQDALKERLL
                     SFWPAYGKTMAAWPSPPPGASLPTKESWLLSLSLDLLQNSPISAGTRLPSTGWWRVCPA
                     PAPWARSGTPRGGWGSASDGEGRWGTGGARSSSLWTSRAAGCWHSSRS"
     misc_feature    complement(3361..3363)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3836..3985
                     /locus_tag="KLK06_RS27385"
                     /old_locus_tag="KLK06_27380"
     CDS             3836..3985
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLK06_RS27385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLK06_27380"
                     /product="hypothetical protein"
                     /protein_id="WP_214638484.1"
                     /transl_table=11
                     /translation="MNLSQAEAFTRALPAPPARRGQAVRDGLDERTDRRRQLNQRDRGH
                     HVLT"
     gene            3972..4874
                     /locus_tag="KLK06_RS27390"
                     /old_locus_tag="KLK06_27385"
     CDS             3972..4874
                     /EC_number="3.1.26.11"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888956.1"
                     /locus_tag="KLK06_RS27390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_27385"
                     /product="ribonuclease Z"
                     /protein_id="WP_214638485.1"
                     /transl_table=11
                     /translation="MSSRELVVLGTSSAVPTRHRNHNGYLLRWDGQGYLFDPGEGTQRQ
                     MVHAGVSANDIHWLCLTHFHGDHCLGVPGVVQRIARDKVRHPVRAVYPASGAEYWQRLR
                     HAAVFADTTVIEEQPISGDHVTIGPLTARRLSHPVESYGYRLQEASSRRMLPDKLASFG
                     IRGPEISELQRTGSVRGITLDQCSVPKAGQSAAVIMDTRLCDAVFELAAGTDLLVIEST
                     FLSAESALAKEYGHLTAAEAGQVAAEAGVRRLVLTHFSERYGFADEPAFLGEARRGYDG
                     EIILARDLMKIPVPRRRHP"
     gene            5001..5816
                     /locus_tag="KLK06_RS27395"
                     /old_locus_tag="KLK06_27390"
     CDS             5001..5816
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545141.1"
                     /locus_tag="KLK06_RS27395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_27390"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_308168463.1"
                     /sec_met_domain="DUF692 (E-value: 1.5e-81, bitscore: 268.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNPSPERLGVGIGWRAELDLTVERLPGVDFVEVIAENIRPSALPE
                     SLRVLRSRGTPVIPHGVSLGIGGAEPPDHRRLAHLAACAQALASPLVSEHLAFVRAGDL
                     EAGHLLPVPRTREALSVIVQNVRQAQESLPVPLALENVAALFGWPEDELTEAEFLRELV
                     ERTGVGLLIDVANLYTSQVNLGLDARAALDTLPLEELAYVHVAGGYARDGVWHDTHTTV
                     IPPDVLDLLAELSARAEPPGVLLEWDADYPSDAVLTAELDRIRAASHAR"
     gene            5806..6270
                     /locus_tag="KLK06_RS27400"
                     /old_locus_tag="KLK06_27395"
     CDS             5806..6270
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLK06_RS27400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLK06_27395"
                     /product="hypothetical protein"
                     /protein_id="WP_214638486.1"
                     /transl_table=11
                     /translation="MPDDHAVDEPRGPDDRALDERRAPDDRVTEERPAPDEHAADQRRQ
                     RLADAQRGLLAALVAGGPLPDGFDPVRLRIEAAGLIAKRRGLVARSAPDLVIRLGPRFA
                     ALFTEYAERRPKPPGGSLGDAKAFADWLGLPLNPPPAGRWARFLRRRPIS"
     gene            complement(6398..7270)
                     /locus_tag="KLK06_RS27405"
                     /old_locus_tag="KLK06_27400"
     CDS             complement(6398..7270)
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854113.1"
                     /locus_tag="KLK06_RS27405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_27400"
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_214638487.1"
                     /transl_table=11
                     /translation="MTTLGAVFRPQLPPERMRGVAHAAEEAGLDELWLWEDCFSQSGIA
                     SAAAALAWTERLRIGVGLLPVPLRNVALTAMEVATLHRLFPGRVVLGVGHGVQEWMAQA
                     GARVDSPLTLLREHLAALRALLRGERVTVDGRYVRLDDVALDWPPETAPPVLAGAVGPR
                     SLRLSGEAADGTILVAGTPPDQVRQARTLIDEGRAAAGRTDRHELIVGLHTAIGPDGPA
                     RLKAELEGWGDAGKPDAGVAGDAGTVAKAVQRLADAGADTVVLQPTADEPDLEGFVRFV
                     AEEIRPLVP"
     gene            complement(7314..7739)
                     /locus_tag="KLK06_RS27410"
                     /old_locus_tag="KLK06_27405"
     CDS             complement(7314..7739)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024212.5"
                     /locus_tag="KLK06_RS27410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_27405"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_214638488.1"
                     /transl_table=11
                     /translation="MEALPPSLLGLTTYLLSKTGKAARGRLAARLAADGLRMWHMAVLA
                     ALADFGPHAQRDLSARLGIDPSDVVKVLDELSAPGFVDRARDPADRRRVTATITAGGRR
                     ELTRLTREARAIQDELLAPLDVAERDQLHALLTRILV"
     misc_feature    7725..7739
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 22.58"
                     /tool="antismash"
     misc_feature    7783..7797
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.32"
                     /tool="antismash"
     gene            7803..8201
                     /locus_tag="KLK06_RS27415"
                     /old_locus_tag="KLK06_27410"
     CDS             7803..8201
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013098.5"
                     /locus_tag="KLK06_RS27415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_27410"
                     /product="VOC family protein"
                     /protein_id="WP_214638489.1"
                     /transl_table=11
                     /translation="MDVLHPRLLVSAFAECFAFYDAVLPEVLGASLAKGGPDGPYAHWD
                     VGSEGVLSMLDRGFMTEVAGTGALPADAAAQDRVMLVSRVADVDRAYAVCLAHGGRSVT
                     EPADRPGWGLRAAHVRDPEGNLVEFQSY"
     gene            8249..9115
                     /locus_tag="KLK06_RS27420"
                     /old_locus_tag="KLK06_27415"
     CDS             8249..9115
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLK06_RS27420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLK06_27415"
                     /product="hypothetical protein"
                     /protein_id="WP_214638490.1"
                     /transl_table=11
                     /translation="MPKRSLALAALAVIVSGCGTTSTAQPSPSAATASQGLGENQRIEL
                     AKADCMKANGFKYVAWIPPALPETEQSRMASARDYEGMKSYRAKYGFGVSATLVYPPSG
                     ETAGQAHEPKNPNVALVKALSSAQRHSYGVKFEKCQITAIKQATGKDVKSADEWSQFRD
                     QAYVERRKRELNGDARLTELAAGMADCMTAKGYQVTSHSPIAMRDWGYTTFTRELHGLA
                     KKEDKNIPDFDPESGTGYIPTHLTEGEKRALLNREIKAALDDLECGKAFHPAAAAKDDQ
                     LSTELTE"
     misc_feature    8264..8266
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(9173..9916)
                     /locus_tag="KLK06_RS27425"
                     /old_locus_tag="KLK06_27420"
     CDS             complement(9173..9916)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892002.1"
                     /locus_tag="KLK06_RS27425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLK06_27420"
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="WP_214638491.1"
                     /sec_met_domain="PEP_mutase (E-value: 2e-75, bitscore:
                     248.3, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTDKAALLRALHVPGNPLVLPNAWDAASARAVQEAGFPVIATGS
                     AAVSQVLGYEDGEGTPVAEMMAAVARITRAVDVPVTADVERGYGLKPAELVERLLTAGA
                     VGCNLEDSDPRTDLLVDVEAQAEFLADVRAAAGSGLVINARVDTYLHGDRTREAAVTRA
                     RRYLEAGADCVYPIVLGDAEEIRALVSEVGGPVNVLFRPGVPDLARLAELGVARVSYGG
                     GLHRATYEYARGLIQAIDEGRSPYE"
     gene            complement(10007..10555)
                     /locus_tag="KLK06_RS27430"
                     /old_locus_tag="KLK06_27425"
     CDS             complement(10007..10555)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLK06_RS27430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLK06_27425"
                     /product="hypothetical protein"
                     /protein_id="WP_214638492.1"
                     /transl_table=11
                     /translation="MQDPMEPDRQEILTGPGDMPRPARNGRSATKKKTVIVAASALVAV
                     LAAGGIGFALAGGPGGPAQPAPSTGQAGQASPTQTAADEPLDEEASMGDAKTDAPDEGL
                     SSDEPAADMGDAVTDPDTSTDTDKGAAGTDSRTTATPPAKNGKKPTSSPTKPAQEPAGD
                     NPADGPAGEVIGQCAKTGC"
ORIGIN
        1 tgagtaccga tgtgccctcc ccgcaccggg gacagtaccg ggaggtgccg cgcgccggga
       61 cggtgaccac cgcgatggcg tgcttggccg ccaagtgccg gatcgcctcg acgatcagcc
      121 cgcgcacctg ccccgacagg cgcgcgttcc agcttcctcg ccgcccgcgc gcctccaagg
      181 tggccagatc ttccacatag aggaccgtcg cccccgcggc cacggcctga tcgaccgccc
      241 atcgcgctgc agaccaggcc agtgccctgt tcagatgccg gatgcgcgca cacagccgct
      301 catgctcaac cccgatgcgc tgccaggccg tactcaacgc gacgtaacgg gaatcagcag
      361 gcgtgatcct cgccagcagc ctttccaggt gccggtgctt ggcggccagg tgctcacgct
      421 cggcccgcag ccggtgcagc ttggccgaca caccgctcgc atcgtaggcg aacggccgcc
      481 catcactgat cacccggcca ccggccaact ccccgaccac gccgaccagc agcccgttca
      541 ggccccagtc gaacccgcac ccgacccgat gaccggtagc gggcgcgaag ccgaccggga
      601 tctggaacgg cagatcgacg cgcacccggc ccttgctcac ccgcacggtg ggggcgcaca
      661 acgctgcctg cggcggcacc gtcggcggca acgcgaccgg cagcacatgc cacgcccagt
      721 ccctggcgga cgcgggcgat tcgaccagcg gaagccgcac ccgcagccgg gcgctgcggt
      781 gaccgatgcg ctgcagggtg agcagttgcc ggtcggccgc cgccagcacc acctgcgccg
      841 caacgttcgg cggctcctcc aactcgatca gatcagccgg caacaccgca tgctcgtcga
      901 ggtaggcgcg gatctgccgg gtccggttgc ggacgtccgc cgccgtcacc ccgtccggca
      961 gcaccttgcg cagcgcgtcc cactcggcgc cggtccgcgc acccggcttc tgcggccaag
     1021 tcgccacgat cgccgccacc aggcggcgcc ggtgcagtgc cagccgcaag gcacgagcgg
     1081 cctgctcctg cgcgcaccgc atcacccgat cgcacacatg aacgaccgcg ggcgggctca
     1141 cgccccagcc gagccggcgc agggccatcc agcccttcga tggcaacacc cgcccgtcca
     1201 gccccacccc ggacgccaaa gcgtccagat cagcagcatt ccaccgggcg gcgaccaggt
     1261 gggcggtcag gtcgcgtgcc aggccgctca gccatgccac ccgccgcacc agcaggcccc
     1321 ccggaacgac ctcgccgctc acctccgcca ccgccgcata ggcggtgcag gaggcggtcg
     1381 cgatgagttc actcatgcac cgctcccgtc tcggaggttg ggccgaagag gacgaaccgc
     1441 gcttggatcg ccgccctctg ggcactgccc gacggccgac ggccggcgac ttcgtgcggg
     1501 cacgagggtg ccagccgggc ggcggtcgtg gcaacaggtg caccacgcgg tcagcgcgtc
     1561 gcgatcggag aggagccggt acacccgcat gcctgcaccc ccgcccctcc gaaacacaac
     1621 ctcacgatcg gtagccgaaa gattaccatt ggttgacctc aacgggtaga acatatgaac
     1681 gacaacgttc cgatctgcct gcatggccaa ggccggccgc agcccgccgt gttgcgggcg
     1741 tgcaggctca gcggcgccgc aggcaaccgg ttcggcgagc gcctcgccaa accatggccc
     1801 gccctgcggc atgatcctgc tcggcccgac cgacaccgcc gaccaagagg aaacctcgac
     1861 agatcatggc cgatgttcag gtcttcatag cgaatatgag cagcagttca taccctcatc
     1921 cgaggcattc gttcctgccg ccattcttcg gggcggggcc atcggcgggg agagtgggag
     1981 gggtgtccgt ccaggtggcc ttctggtaga cgagcgcgtg gtagatcgtc cccgcgggca
     2041 gaccggcaag cccctcgtca ccgggcggat tccgcgacgc ctgctcgact acgacctgct
     2101 ggagtgccag cagcctgccg ctcgactcgt ccacaatgag ctgctcctcg ctccgcccgt
     2161 cccccatcgt ccctcgccat ccgatgccga tccccagccg cccctcggcg tcccggacct
     2221 ggcccagggc gcgggtgccg ggcagactcg ccaccatccg gtagagggca ggcgggtgcc
     2281 cgccgagatc ggggagttct ggagcaagtc cagcgacagg ctcaggagcc aggactcctt
     2341 ggtgggcagc gaggcccccg ggggtggtga cggccaggcg gccatggtct tcccgtaggc
     2401 gggccagaac gacagcagcc gctccttcag cgcgtcctga tcgcgaggta gatcgagcag
     2461 ttcgctggcc gaaagggtga ggccctgatc ttgaatcggc cacgtactgg gtctgaacat
     2521 gaatctggta cgtccgagcg gcgccgtgtc gttcccgcag tcggagtcga cgccgcagag
     2581 cttgggcgcg cccgcccgcc gccatgcctg ctcgttcgcc gccgaggccc gtacggtggg
     2641 catgcctcgc tcggtgaaca gcatgtatgc ctccgggccg cgcggcaccc accggctcgt
     2701 ctggacacgg gtcttgatcc gataccggtt gtccggtgag ccgatgacct gctccccgag
     2761 aatcaggccc acggtgtgcc agtagcgtcc gggcacctgt tgctgcccgg acgcacggtc
     2821 cgcagccacg tccaggacgt ttgcggcgga tcggcccgtc acctgcccgg tgacggtgtc
     2881 gtgacccaac agccggggaa cccccagcac ggcgatcact gacgccatcg cgagggaggc
     2941 cacgaccgct ccaacccatc tgacctgccg ccctctgacc ggcgcgccca actccacgac
     3001 ctgtgcgcga ggtgtcccgg gcgggtcgag gtcggcgggg cgggcggccg cgaggacgtc
     3061 caggacgttc ttccgcttca ctgctcctcc tccgatacca gcgcgcgctg accccgtacc
     3121 gccgtcaagc cgtccatggc cgccgccaga cgccgcctgg cgcgatgcag ccgcacgaag
     3181 aaggccgacc ggggagctcc caaccacctt ggccgcctcc acggagctca gcccgtgcca
     3241 cgaaaccagc atcagcacct ccttgtcctc gtcgctcaac gtggccagcg ccctcaacac
     3301 ggccgcgcgc tccgccacct cttcggccac atcgggcccg gtctcgtcgg cccagccgtg
     3361 taacgcgtcg tccagcgcca cccgccgaag ctcctcccgg tggacgtcac ggatcacgtt
     3421 gcgcgcgacg cccagcagcc acggcagcgc ggccttggcc ggaatgtcgt ctcgccgccg
     3481 ccaggcgacc acgaacgtct cactgaccac ctcctcggag agccggcggc cacatctgct
     3541 caccgcatac gcgtacacgc ggggatggca cgcatcgaac aacgcggcga acccctcttg
     3601 gttcagcacc caggctcctt cactgacact tcaccaggta gtggtcgaag gcgggccggt
     3661 cttaccactg tccacaagat ttctgccgcg tccttgcgtc acgtccgtgc aaattctctg
     3721 gtgaaggggt tcggcgggtc ttctcccaga cgtgttccca gatcactaca tgaaggggac
     3781 aactgagccg ttaagcgttc cgctcggcga cggcgaaagc cgtacgatcc gggtcatgaa
     3841 cctcagccag gccgaggcgt tcacccgggc gctcccggcg ccccctgccc gacgtggcca
     3901 ggccgtccgt gacggcctag acgagcggac cgatcggcgt cggcagctca accaacgcga
     3961 ccgtggccat catgtcctca cgtgagctcg tggtgctcgg cacctccagc gcggttccaa
     4021 cccgacaccg taatcacaac ggatacctgc tgcgctggga cggccagggg tatctgttcg
     4081 acccaggtga gggcacacag cggcagatgg tgcacgccgg ggtcagcgcc aacgacattc
     4141 actggctgtg cctgacgcac ttccacggcg accactgcct gggcgtgccg ggcgtggtgc
     4201 agcgcatcgc gcgcgacaag gtccggcatc ccgtacgagc cgtctacccc gcctccggcg
     4261 ccgaatactg gcagcggcta cgtcacgccg ccgtcttcgc cgacaccacc gtcatcgaag
     4321 agcagccgat cagcggcgat cacgtcacca tcggcccgct gaccgcgcgc cgcctgtccc
     4381 accccgtcga gtcctacggc taccgcctcc aggaggcgtc cagtcgccgc atgctgcccg
     4441 acaagctcgc gtcgttcggg atccgcgggc ccgagatcag cgagctccag cgcaccgggt
     4501 ccgtccgcgg catcacgctc gaccagtgca gcgtccccaa agccgggcag agcgccgccg
     4561 tcatcatgga cacccgcctg tgcgacgccg tcttcgaact cgccgctggc accgacctgc
     4621 tggtgatcga gtcgacgttc ctgtccgccg agagcgctct ggccaaggag tacggccacc
     4681 tcaccgcggc cgaggccggc caggtggcgg ccgaggccgg agtgcgccgc ctggtgctga
     4741 cccacttctc cgaacgctac ggcttcgccg acgaacccgc cttcctcggc gaggcacggc
     4801 gcggctacga cggcgagatc atcctggccc gcgacctgat gaaaatcccc gtaccaagac
     4861 gacggcaccc gtaggaaact ccgcgacgac cgcgttcgtc attcaggcga gaacacccct
     4921 cgacacgggg aggcccctat ggaggaagtg ctttacacgg ccggctgcgg cggtggttcg
     4981 agctgcgggg gaggcagccc gtgaacccca gcccggagcg gctgggcgtg ggcatcggct
     5041 ggcgggccga gctcgacctg accgtggaaa ggctcccggg ggtggacttc gtggaggtca
     5101 tcgcggagaa catccgcccg tcggcgctgc ccgagtcgct gcgcgtgctc cgttcccggg
     5161 gcacgcccgt gatcccgcac ggcgtgagcc tcggcatcgg cggcgccgaa ccgcccgatc
     5221 accggcgcct cgcccatctc gccgcctgcg cccaggctct cgcatcaccg ctggtcagcg
     5281 agcacctcgc cttcgtcagg gccggagacc tggaagccgg ccacctgctc ccggtaccgc
     5341 gcacgcgtga ggcactgtcg gtgatcgtcc agaacgtacg gcaggcgcag gagtccctgc
     5401 cggtgcccct cgccctggag aacgtggcgg cgctgttcgg ctggccggag gacgagctga
     5461 ccgaggccga gttcctgcgt gagctggtcg agcggaccgg tgtcgggctg ctcatcgatg
     5521 tggccaatct ctacaccagc caggtcaacc tgggcctcga cgcccgcgcg gccctcgaca
     5581 cgctgcccct ggaggagctg gcctacgtcc acgtcgctgg agggtacgcg cgcgacggcg
     5641 tatggcacga cacgcacacg accgtcatac cgccggacgt cctcgacctg ctcgccgagc
     5701 tgtccgcccg cgcggaaccg cccggtgtgc tgctggagtg ggacgcggac tacccgagcg
     5761 acgccgtact caccgccgaa ctcgaccgga tcagggcggc gtcacatgcc cgatgaccac
     5821 gctgtggacg aaccccgcgg accggatgac cgcgccttgg acgagcggcg cgcgcctgat
     5881 gaccgcgtta cggaggaacg gcccgcgccc gatgagcacg ccgccgacca gcggcggcag
     5941 cggctggcgg acgcgcagcg ggggcttctg gcggcgctcg tcgccggtgg gccgctccct
     6001 gacggcttcg acccggtacg gctccggatc gaggcggccg ggctgatcgc caagaggcgc
     6061 ggcctcgtgg cgaggtctgc tccggacctg gtgatccggc tcggcccccg attcgccgcg
     6121 ctcttcaccg agtacgccga gcggcgcccc aagccccccg gcggctccct gggcgacgcc
     6181 aaggccttcg ccgattggct cggcctgccg ctgaaccccc caccggccgg gcgatgggcc
     6241 cgcttcctcc gccgccggcc gatttcctag gccggccagc ccgctttcca ggccagtcgg
     6301 cttgcttcct aggtcggccg acccgctttc cgggtcagtc ggcctgcttc gtaggccggc
     6361 cgacccgctc tcccggccag tcggcctgcc tcgtacgtca gggaacgagc ggccggatct
     6421 cttcggccac gaaccgtacg aagccctcga ggtcgggctc gtcggccgtc ggctggagca
     6481 ccacggtgtc ggccccggcg tccgccagcc gctggacggc cttggcgacc gtgcccgcgt
     6541 ccccggcgac tccggcgtcc ggtttgccgg cgtcgcccca gccctcgagt tcggccttga
     6601 gtcgtgcggg tccgtccgga ccgatcgccg tgtggaggcc gacgatcagc tcgtgccggt
     6661 cggtacgccc ggccgccgca cgcccttcgt cgatgagcgt ccgagcctgt cgtacctggt
     6721 cgggcggggt accggcgaca aggatggtgc cgtcggcggc ctcgccggac agccgcagcg
     6781 accgcggccc gaccgccccg gcgagcaccg gtggcgccgt ctccggcggc cagtcgaggg
     6841 cgacgtcgtc gagccgtacg taacggccgt ccacggtgac ccgctcgccg cgcagcagcg
     6901 cccgtagcgc cgcgaggtgc tcgcgcagca gcgtcagggg cgagtccacc cgcgcgccgg
     6961 cctgggccat ccactcctgt acgccgtggc cgacgcccag gacaacccgc cccgggaaca
     7021 gccggtgcag cgtggccacc tccatcgcgg tcagggcgac gttgcgcagg ggcaccggga
     7081 gcaggccgac gccgatgcgc agccgttcgg tccaggcaag ggccgcggcc gcgctggcga
     7141 tgccgctctg ggagaagcag tcctcccaga gccagagttc gtccagcccc gcctcctccg
     7201 ccgcgtgggc cacgccgcgc atccgttcgg gagggagctg aggccggaac acggcgccga
     7261 gagtagtcat gaggtctgtc ctacctgctc cgcagccgga tggacaacag gagctagacc
     7321 aggatccggg tgagcagggc gtggagctgg tcgcgttcgg cgacgtcgag gggggcgagg
     7381 agctcgtcct ggatggctcg cgcctcccgc gtcaggcggg tgagttcgcg ccgtcccccg
     7441 gcggtgatgg tggcggtgac cctgcggcgg tcggcggggt cgcgggcgcg gtcgacgaag
     7501 ccgggcgccg agagctcgtc caggaccttg accacgtcgc tggggtcgat gccgagccgg
     7561 gccgaaaggt cgcgctgggc gtgcggcccg aagtccgcca acgccgccag cacggccatg
     7621 tgccacatac gcagcccatc tgccgccagc cgcgcggcca gccggcctct ggccgccttc
     7681 ccggtcttcg acagcaggta cgtggtgagt ccgagcaggc tcggcggcag cgcttccatc
     7741 ctagatattg tagggtctga cccaataata ggtcttcacc cactatttca ggagatttcc
     7801 ggatggacgt gctgcatcct cggctgctgg tttcggcgtt cgcggagtgt ttcgccttct
     7861 atgacgcggt cctgccagag gtgctcgggg cgagcctcgc gaagggcggc ccggacggtc
     7921 cctacgcgca ctgggacgtg gggtcggagg gggtgctgtc gatgctcgac cgcggcttca
     7981 tgaccgaggt cgcgggcacc ggcgccctgc ctgcggacgc cgccgcacag gatcgcgtca
     8041 tgctcgtcag ccgggtggcc gacgtcgacc gggcgtacgc cgtctgcctg gcccacggcg
     8101 gccggtccgt cacggagccc gcggaccggc ccggctgggg cctgcgggcc gctcacgtcc
     8161 gtgacccgga aggcaatctg gtggagttcc agtcctactg agctcgatga ttgacaatac
     8221 tacgtttaac gaattatggt ccgaggtcat gcccaagaga tcattagcgc tggcggcgct
     8281 cgctgtcatc gtctccggct gcgggacgac cagcactgca cagccgtctc catcggccgc
     8341 cacggccagt caggggcttg gcgagaacca gcggatcgag ctggccaaag ccgactgcat
     8401 gaaggcgaac ggcttcaaat acgtagcgtg gatccctcct gcgctgcccg agacggaaca
     8461 atccaggatg gcctccgccc gcgactacga gggcatgaag tcataccgcg ccaaatacgg
     8521 cttcggggtg tccgccacgc tcgtctaccc gccgagcggc gagaccgcgg ggcaggccca
     8581 cgagcccaag aaccctaacg tcgccctcgt caaggctctc tcgtctgctc agcgccattc
     8641 gtacggggtc aagttcgaaa aatgtcagat cactgcgatc aagcaggcca cgggcaagga
     8701 cgtgaagtcg gctgatgagt ggagccagtt ccgtgaccag gcatacgtag agcgcaggaa
     8761 gcgcgaactc aacggcgacg cccggctcac cgaactggct gcgggcatgg ccgattgcat
     8821 gaccgccaag ggctaccagg tcacgtcgca cagcccgata gccatgcgtg actggggata
     8881 tacgacgttc accagggaat tgcacgggct cgcgaagaaa gaagacaaga acattcccga
     8941 cttcgacccg gagtccggca caggctacat cccgacgcac ctcacggagg gcgagaagcg
     9001 ggcccttctg aaccgagaga tcaaggcggc gctcgacgac ctcgaatgcg gcaaggcctt
     9061 ccaccctgcc gcggcagcca aggatgacca gctcagcacc gagctgaccg agtaacgcga
     9121 aactttccct ccgccctcgt ggattcgaaa ggaaggctcc tcgtgaacgc ggtcactcgt
     9181 acgggctgcg gccctcgtcg atcgcctgga tcaggccgcg agcgtactcg tacgtggccc
     9241 ggtgcaaccc gcccccgtag ctgaccctgg ccacgcccaa ctcggccagc cgggccagat
     9301 cggggacgcc gggcctgaac agcacgttca ccgggccgcc gacctcgctc accagggccc
     9361 ggatctcctc cgcgtcgccg aggacgatcg ggtagacaca gtcggccccg gcctccaggt
     9421 agcgccgtgc ccgcgtcacg gccgcctccc gtgtccggtc gccgtgcaga taggtgtcga
     9481 ccctggcgtt gatcacgagc cccgatccgg cggcggcccg cacgtccgcc aggaactcgg
     9541 cctgcgcctc cacgtcgacg agcaggtcgg tgcgggggtc ggagtcctcc aggttgcagc
     9601 cgacggcacc ggccgtgagc aggcgttcga ccagctcggc cggcttcagg ccgtacccgc
     9661 gctcgacgtc cgccgtcacg ggcacgtcca cggcgcgcgt gatgcgggcc acggcggcca
     9721 tcatctcggc gacgggggtc ccctcgccgt cctcgtagcc cagcacctgg gagacagccg
     9781 cgcttccggt ggcgatcacc ggaaacccgg cctcctgtac ggcccgcgcg gacgccgcgt
     9841 cccaggcgtt gggcagcacc aacgggttcc cgggcacgtg gagagcccgg agcagggcgg
     9901 ccttgtcagt ggtcataccg ggcagcctag gaaggaagtg gctcccgcca aagggccaat
     9961 accgcgggga accgccaatc cacttggcag ctcacccact cagccgtcag caaccggtct
    10021 tcgcgcactg cccgatgacc tcgcccgccg ggccgtccgc cgggttgtcg cccgctggct
    10081 cctgcgccgg tttggtcggc gaggacgtgg gcttcttgcc gttcttggcc ggaggcgtgg
    10141 ccgtagtgcg tgaatcggtg cccgccgccc ccttgtccgt atccgtgctc gtatccgggt
    10201 ccgtgaccgc gtcgcccatg tcggcggcgg gttcgtcgga cgagagtcct tcgtcggggg
    10261 cgtccgtctt ggcgtcgccc atggacgcct cttcatccag cggttcgtcg gcggcggtct
    10321 gagtggggct cgcctggccg gcctggccgg tggagggcgc gggctgggcg ggacctccgg
    10381 ggccacccgc cagcgcgaag ccgattccgc ccgccgcgag cacggcgacg agggccgacg
    10441 cggcgacgat gacggtcttc ttcttggtgg cggatctgcc atttctggca ggtctcggca
    10501 tatcgcccgg ccccgtgagg atctcctggc gatccggctc catcggatct tgcatggtgg
    10561 tgcgactccc tgcggtcgta tcgggaaagc tcgccggctt ccgcgggtga tggaccacac
    10621 tagccgcagg agtcgaatgc tcgcgcacca atcacaaagg aatcacgact tgtcaccgca
    10681 taaagccatt cgcccaggtc gtcgtgaccg cctggcagcc gcggcaggtg tggccggggt
    10741 gatcgtcgtc gccgcgctcg gtgttttcct gtcgctcatg ggatctcaaa gtcccgagcc
    10801 gaaaaagggg ccggaa
//
