LOCUS       NZ_JAHFZY010000003     11884 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Actinomadura sp. NEAU-AAG7 Scaffold3, whole genome shotgun sequence.
ACCESSION   NZ_JAHFZY010000003
VERSION     NZ_JAHFZY010000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura sp. NEAU-AAG7
  ORGANISM  Actinomadura sp. NEAU-AAG7
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 977316)
  AUTHORS   Xu,X. and Zhao,J.
  TITLE     Actinomadura oryzae sp. nov., a novel actinobacterium with the
            activity of releasig potassium isolated from rhizosphere soil of
            rice in Sanya
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 977316)
  AUTHORS   Xu,X. and Zhao,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2021) College of Life Sciences, Northeast
            Agricultural University, 600 Changjiang Road, Xiangfang District,
            Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018603865.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 03:10:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,952
            CDSs (total)                      :: 7,871
            Genes (coding)                    :: 7,813
            CDSs (with protein)               :: 7,813
            Genes (RNA)                       :: 81
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 6 of 58
            Pseudo Genes (incomplete)         :: 53 of 58
            Pseudo Genes (internal stop)      :: 1 of 58
            Pseudo Genes (multiple problems)  :: 2 of 58
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:01:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 851985
            Orig. end                         :: 863869
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHFZY010000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11884
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11884
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11884
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6884](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6884
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            987..3380
                     /locus_tag="KLI87_RS18515"
                     /old_locus_tag="KLI87_18520"
     CDS             987..3380
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012588.5"
                     /locus_tag="KLI87_RS18515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_18520"
                     /product="phosphotransferase"
                     /protein_id="WP_214617996.1"
                     /transl_table=11
                     /translation="MTETIEPGAVEYPALPGRGEYTEEARRARLEWLRAATGAALGSLE
                     TSTIDPRSLTGNVENFVGGIEIPIGLAGPLLFDGQRARGLVTAPLATTEGAMVASVSRG
                     AKAITLAGGVASRVISQRMTRAPVFEFAGLGEAAEFARWVVGRKDALDRQIAAVSTHSR
                     LVELDPVQLGRSVHLRFVYETAEAAGQNMTTVATWRACRWIHDQLAAGSVPVPVWSAIE
                     ANLSGDKKFTRLNMIAGRGTRVVAECVLDEGTVRRVLKTTPGAIDRLYRISVVAAQQAG
                     MAGHDIDAANVIAALFVATGQDIACVHESGAGLFSVDVSGGELRATLVLPSLVTGAVGG
                     GTGLAPQRDCLEALGCTGEGGAARFAEITCGFALALNLSTMAAITSGQFADAHERLGRG
                     RRVNWLRPEDLGPALLRPMLAASLDAPGLDVTAVAPIEDAPRSSLITELTSRGERRKTT
                     GVFPLRVTWVRRDGDDPKQTDLVVKVKPLDQEVAIEAAKLASLCGGRLAEVYPRWRDAT
                     GFGGVHTREPAVYRSADPALRAVLPETYGVFEDPAREAHVIVMERLGTGVILKDTVDEP
                     EAWRPRHIAAALRGIASVHAAWLGREEEVRGWLGAARTAESMTAMRELWSAHLEHSAAE
                     HPDLLDACAARRLEDVLDGLPDWWRRMEAMPRTLVHNDFNPRNIALRAADLSLVAYDWE
                     LATLHVPQRDLAELLAFVLSPEASEADVTAYLDLHREAVGAGIDPAAWRDGYRLALWDF
                     ALTRLQMYLMVHTHRELPFLRHVVPTALRLIEIEDAVEEAGRAG"
     gene            3370..4209
                     /locus_tag="KLI87_RS18520"
                     /old_locus_tag="KLI87_18525"
     CDS             3370..4209
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLI87_RS18520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLI87_18525"
                     /product="hypothetical protein"
                     /protein_id="WP_214617997.1"
                     /transl_table=11
                     /translation="MPADRPGPLRLAILGIDGSGKSTVARCLAERSPIGGRHAVLSCVR
                     PHENPDAPLHELSRHLDLLSKEADRLHSPELKLAVLYLQMCTYGVSERFFVRELGADVV
                     ISERHPVIDALAYLPLYRSAIVRLAGPGRADAVREGLAALPAASVEAATAWCEALGRRS
                     GRPAPRLDTLAGELVALPDRPAAEQIAEFRDRFGIGLPDIAVLLDVDVATAADRIGARD
                     RRAEQHERPDRLTRLRDGYERALPAFGSVTIHRIQVGARPPGDVAAEIARLTAAGTR"
     gene            4206..4871
                     /locus_tag="KLI87_RS18525"
                     /old_locus_tag="KLI87_18530"
     CDS             4206..4871
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039369.4"
                     /locus_tag="KLI87_RS18525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_18530"
                     /product="DUF6182 family protein"
                     /protein_id="WP_214617998.1"
                     /transl_table=11
                     /translation="MSGPPRAALGAEWDARLERVRLASRAVRGHLPRTAAVAVLRGFTA
                     REFLGSAVAFAAGLPPDRRAAWYGSYSRTIFLAGDPRNLAERHPCDHLSDDGSIAWYAP
                     APMADREGLRRLLRPFHGPLGVTGPAEEEIPVGEGGGVARLEVPVADLPVENYLVNVNH
                     LLAEAAMDGLFTGIGRLLVRHLPRDPDERAIRWDRIRVSPDDRSAGTFRAHAYLALSP"
     gene            5001..6884
                     /locus_tag="KLI87_RS18530"
                     /old_locus_tag="KLI87_18535"
     CDS             5001..6884
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /locus_tag="KLI87_RS18530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_18535"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_214617999.1"
                     /sec_met_domain="TIGR03975 (E-value: 6.2e-181, bitscore:
                     598.5, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVVLVNMPWGSTDVPSLALGILKRAVAERIPEAEVEVLYANIDF
                     VDWVHDRLDMAMDDYDFFAQRSFTEGNGDWVFSSALYDDPDWRRREFDERTSGEKADLA
                     LCHRLHELAPAFVAELAGRVAASAPDVVGFTSTFQQNVAALAAARQVKRLRPETVTILG
                     GASCDGPQGAALHRNFPFVDFVLRGEGEVAFPRFLTAVAGARDFAAIPGLVWRDGSGAS
                     IANAMSARPLAPSEIVAPDYDDYFRRYATSVARLWADPRLVLESSRGCWWGEKHHCGFC
                     GLNGSFMEFRSKPPDRFLDEMLALARRHQVLDVYMVDNILDMGYLTSMLPALAAEGYDF
                     RMFYEVKSNLRRQQVRALADAGVVRVQAGIESLSGRVLGIMDKGVTGCQNVRMMRDAES
                     AGVWVEWNYLYGFPGEDAGDYTSVVEQFPALHHLPPPGIVGRLAIERFSPYFDRPELGF
                     AELRPHPQYSMIYDLPEAELFDLAYLFEAPPRGIGAAVAGRVADAAAEWQAAYPESRLT
                     MCDLGDHIDLVNRRPAFGWTALRVEDPVEMAALRLLDRPHRPAALAGKLAAVDPAVTVD
                     GVADLLARWRGLGLTFADGGQVVLTAADAAGQGRRRVRRRAADPADLVGAP"
     gene            6884..7492
                     /locus_tag="KLI87_RS18535"
                     /old_locus_tag="KLI87_18540"
     CDS             6884..7492
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039872.4"
                     /locus_tag="KLI87_RS18535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_18540"
                     /product="DUF5825 family protein"
                     /protein_id="WP_214618000.1"
                     /transl_table=11
                     /translation="MAPVTMTLWRDYEELDRPGVCAGTAAVSGPAAEASRALFEDGVRR
                     VALKDTVDLSGATDAVPSLVLVRELTAYGIAVDWRVRLGDGRWRDLSHLYPPGEVVGDE
                     EARRDWRDGYHFFKCVYRRGPGFLQVRDRRSGVLRKITIDGPAHRAAVESLLEGCPSAA
                     LPPPVLRDLRTMRLVHFLDGAAWWLPCRVRRWPAGKSVL"
     gene            7526..7699
                     /locus_tag="KLI87_RS18540"
                     /old_locus_tag="KLI87_18545"
     CDS             7526..7699
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLI87_RS18540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLI87_18545"
                     /product="hypothetical protein"
                     /protein_id="WP_156218239.1"
                     /transl_table=11
                     /translation="MTVKRPEEAGRESAREEALRAALAEMSTENLATTEGIRVDVVRDD
                     IDAPHERDWGIG"
     gene            7803..8984
                     /locus_tag="KLI87_RS18545"
                     /old_locus_tag="KLI87_18550"
     CDS             7803..8984
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLI87_RS18545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLI87_18550"
                     /product="hypothetical protein"
                     /protein_id="WP_214618001.1"
                     /transl_table=11
                     /translation="MTAGTPDAAVWDGVSTLLDHYIRIRPDDRVVLLYTADARDPAAWV
                     AAELAVRGVRPALLGMSAGRDDTVEPRLRAALPDPASPHPRIVLLTMERDSMSHVLQLR
                     RALAPYPEDRWATYRVINASAEFFRHSMGVTPELLSALNGALLNRMMPASRLRVTSGGG
                     TDLEIAVDSERYRWISNRGVWRAGAFLVLPPGEVSTYPADVNGVLVADGAFNVTSYTGH
                     DVRLADHPVTVEIEHGRVTGAACADPLVARLLERCLRVENADLIGELGFGTNVGTTAFI
                     PLNSHINERHPGVHLGLGMHGQRLDVVDYWCDVHLDLIAADATVAVDGRTEIRSADLRG
                     FAGERHPEPLTGNVHEEDIDGDCCGLITGEVARAREEFLRTRECRRTRATGGS"
     gene            complement(8968..10272)
                     /gene="rfbH"
                     /locus_tag="KLI87_RS18550"
                     /old_locus_tag="KLI87_18555"
     CDS             complement(8968..10272)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rfbH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571002.1"
                     /locus_tag="KLI87_RS18550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_18555"
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="WP_214618002.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 4.8e-95,
                     bitscore: 314.6, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDSQDSILELVRKYHREKEEARRFVPGETLVMPSGADLSEDDRV
                     ALVEAALNLRIASGPSAVALERELGRTLRRRKVHLTNSGSSANLLALTALTQPEIGDDR
                     LRPGDEIVTVAAGFPTTVNPILQNQMTPVFVDVELGTYNTTADRVAEAIGPRTRGIMIA
                     HALGNPFPVAEVAELAAERGLFFVEDNCDGVGAGYQGRLTGTFGHFSTVSFYPAHHLTM
                     GEGGCVTTDRPRLARIVESLRDWGRDCWCEPGDCDTCGKRYTQRFGELPYGYDHKYTFS
                     HVGYNLKTTDLQAALGLSQLQRLPEFGAARRRNWARLREGLDGLPGLLLPVPTPGSDPS
                     WFGFVITVTADAPFDRDEIVNHLEDRRIGTRMLFAGNLTRQPAYQGRPYRVSGKLDNSD
                     AITERTFWIGVFPGLTEEMVDYMITTIRDFVTSRR"
     gene            complement(10363..10818)
                     /locus_tag="KLI87_RS18555"
                     /old_locus_tag="KLI87_18560"
     CDS             complement(10363..10818)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851748.1"
                     /locus_tag="KLI87_RS18555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_18560"
                     /product="cupin domain-containing protein"
                     /protein_id="WP_308166063.1"
                     /transl_table=11
                     /translation="MTDARARGTAAWHGRIEHVRGSDLGSDTVQTSGMRRYEAISGKSV
                     GSERLWMGRTHVGPATSSGDHHHGEAETAIYIVSGHPVFVFADGEDEVRVETAPGDYIF
                     VPPYVPHREENPSPDEEAVVVIARSTQEGIVVNLPSLWADVGRPAES"
     gene            complement(10852..11277)
                     /locus_tag="KLI87_RS18560"
                     /old_locus_tag="KLI87_18565"
     CDS             complement(10852..11277)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653747.1"
                     /locus_tag="KLI87_RS18560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_18565"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_156218236.1"
                     /transl_table=11
                     /translation="MAARSRLPDDVEPDACAAFRDALDRVGSKWGIAIIAAAGDGPIRF
                     AELHRSIPGISRRMLTLTLRTLERDGLITRTVHPTVPPQVEYTATALAEELFASTRALS
                     AWAENNHAKIAAARAAYDDNEAARHDDRRPVSGPNLY"
     misc_feature    complement(11478..11492)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
ORIGIN
        1 catcgccgcc gcgctgcccc ggcagctgcg gcactggggg gcccggatgg tcgcggagat
       61 cagggacggg acgctcggcg gctccccgtt ccgggagccg aacccggcgc gggccgacga
      121 gctggagcgc gccgcccggc gcggcggcgg gctccgcccg gtggacctct ggccccggct
      181 ggagctcctc ctgacctgga acacctatct ctcccggctc tacctccccg gcctcctcga
      241 cgactacggc ccgaaggtcc gggtgctccc cgcgccgatc gggtcgtcgg agggtccgct
      301 cgccgtcccg gtggacgacc gtccggcggg cggccccctg gtcgtgacga gctgcttcta
      361 cgagttcgtc ccggccgagg aggagatcga cccgcgcggc ccgacgctgt cggcgcacga
      421 gttggaggag gggcgcgagt accacgtgat cctctcccat ctcggcgggc tctaccgatg
      481 cgcggtccgc gacatcgtga aggtgaccgg gttcgtcgag gcgaccccgg tcgtggagta
      541 cgcgtgccgc aagggactgc tctcggtcgc gggggagcgg ctgcgcgagt cgcagatgct
      601 ccgcgcgctg gcggcggcgg ccgaggcgac gggactgggc gtccgcaacg tcctgtgcag
      661 ggccgacgcc gagcagcccc ggcatgaggt cgcggtcgcc ttccgtgagc cgccggccgg
      721 gcccggcgcg gcggcgttcg gcgcggccct cgaccgggcc ctgcgcgcgg agtcgcccgc
      781 ctacgccgcc gcccgcgacg ccgaggccct cgcgccgccg ctggtcaggg cggtggaccc
      841 cgaggggttc tggcgggagt ggcggcgccg cgtccaggcg ggggagcggc cgccgcgcgt
      901 gaaggaccgg gtgttccagg acgaccccga ggtctgggcg cgggtgaccg ccgccccggg
      961 cacccaggac gaggggagca cgacccgtga ctgagaccat cgaacccggc gccgtcgagt
     1021 accccgccct ccccgggcgc ggcgagtaca ccgaggaggc gcggcgggcc cggctggagt
     1081 ggctgcgggc ggcgacgggc gccgcgctcg gctcgctgga gacctcgacg atcgaccccc
     1141 gctcgctgac cgggaacgtg gagaacttcg tcggcggcat cgagatcccg atcggcctcg
     1201 ccgggccgct gctgttcgac gggcagcggg cgcgcggcct ggtcacggcg ccgctcgcca
     1261 ccaccgaggg cgccatggtc gcctcggtgt cgcgcggcgc gaaggcgatc acgctggcgg
     1321 gcggggtggc gagccgggtc atctcgcagc ggatgacccg ggcgccggtg ttcgagttcg
     1381 cgggcctcgg cgaggcggcg gagttcgccc gctgggtcgt cggccgcaag gacgcgctcg
     1441 accggcagat cgcggcggtg tccacgcact cgcggctggt cgagctggac ccggtgcagc
     1501 tcggccggtc cgtccacctg cggttcgtgt acgagacggc cgaggccgcc gggcagaaca
     1561 tgacgacggt cgcgacctgg cgggcctgcc ggtggatcca cgaccaactg gccgccgggt
     1621 cggtgcccgt gccggtgtgg tcggcgatcg aggcgaacct gagcggcgac aagaagttca
     1681 cccggctgaa catgatcgcc ggacgcggca cccgtgtcgt ggccgagtgc gtgctggacg
     1741 aggggacggt ccgccgcgtc ctcaagacca ccccgggcgc catcgaccgg ctctaccgga
     1801 tcagcgtggt cgccgcgcag caggcgggca tggccgggca cgacatcgac gccgccaacg
     1861 tgatcgcggc gctgttcgtc gcgaccggcc aggacatcgc ctgcgtccac gagtccggcg
     1921 ccgggctgtt ctcggtggac gtctccggcg gtgagctgcg ggcgacgctc gtcctgccga
     1981 gcctggtcac cggcgcggtc ggcggcggca cgggcctcgc gccgcagcgc gactgcctgg
     2041 aggcgctcgg ctgcaccggc gagggcggcg ccgcgcgctt cgcggagatc acctgcgggt
     2101 tcgcgctcgc gctcaacctc tcgacgatgg cggcgatcac cagcggccag ttcgccgacg
     2161 cgcacgagcg gctcggccgc gggcggcggg tgaactggct gcgtcccgag gacctcggcc
     2221 cggcgctgct gcggccgatg ctcgccgcgt cgctcgacgc ccccgggctg gacgtcaccg
     2281 ccgtcgcccc gatcgaggac gccccccggt cgagcctcat caccgagctg acctcgcgcg
     2341 gggagcggcg caagacgacg ggggtgttcc cgctccgcgt cacctgggtc cggcgggacg
     2401 gcgacgaccc caagcagacg gacctggtcg tcaaggtgaa gccgctcgac caggaggtgg
     2461 ccatcgaggc ggcgaagctg gcctcgctgt gcggcggccg cctcgccgag gtctacccgc
     2521 gctggcgcga cgcgaccggg ttcggcggcg tgcacacccg ggagcccgcc gtctaccggt
     2581 cggccgatcc cgcgctgcgc gccgtcctgc ccgagaccta cggcgtcttc gaggacccgg
     2641 cgcgcgaggc ccacgtgatc gtgatggagc ggctcggcac gggcgtgatc ctcaaggaca
     2701 ccgtcgacga gccggaggcg tggcggccgc ggcacatcgc cgccgccctc cggggcatcg
     2761 cgtccgtcca cgccgcctgg ctcgggcgcg aggaggaggt gcgcggctgg ctcggcgccg
     2821 cccggacggc cgagagcatg acggccatgc gcgagctgtg gtcggcgcac ctggagcaca
     2881 gcgcggccga gcacccggac ctgctggacg cctgcgccgc gcggcggctg gaggacgtcc
     2941 tcgacgggct gccggactgg tggcgccgga tggaggcgat gccgcgcacg ctcgtccaca
     3001 acgacttcaa cccgcgcaac atcgcgctgc gcgccgccga cctgagcctg gtcgcctacg
     3061 actgggagct ggcgacgctc cacgtcccgc agcgcgacct cgccgaactg ctcgcgttcg
     3121 tgctctcgcc ggaggcgtcc gaggcggacg tgaccgccta cctcgacctc caccgggagg
     3181 cggtcggggc cgggatcgac ccggccgcct ggcgggacgg gtaccgcctc gccctgtggg
     3241 acttcgccct cacgcggctc cagatgtacc tgatggtgca cacccaccgc gagctgccgt
     3301 tcctgcggca cgtcgtcccc acggcgctgc ggctgatcga gatcgaggac gccgtggagg
     3361 aggcgggccg tgccggctga ccgtcccggt ccgctgcggc tggcgatcct cgggatcgac
     3421 ggctcgggca agagcacggt cgcccggtgc ctcgcggagc ggtcgcccat cggcgggcgg
     3481 cacgccgtcc tgtcgtgcgt gcgcccccac gagaaccccg acgcgccgct gcacgagctg
     3541 tcgcggcacc tcgacctcct gtccaaggag gccgaccggc tccacagccc ggagctgaag
     3601 ctggccgtcc tctacctcca gatgtgcacc tacggggtca gcgagcggtt cttcgtccgc
     3661 gagctggggg cggacgtcgt catcagcgag cgccaccccg tcatcgacgc gctcgcctac
     3721 ctgccgctgt accggagcgc gatcgtccgc ctcgccgggc ccggccgcgc ggacgccgtc
     3781 cgggaggggc tggccgcgct gcccgccgcg tcggtcgagg ccgcgaccgc ctggtgcgag
     3841 gcgctcggac ggcgctccgg gcggcccgcg ccgcggctgg acacgctcgc cggtgagctg
     3901 gtcgcgctgc cggaccgccc ggcggccgag cagatcgccg agttccggga ccggttcggg
     3961 atcggcctgc cggacatcgc cgtgctgctc gacgtcgacg tcgcgaccgc cgccgaccgg
     4021 atcggcgccc gcgaccgccg cgccgagcag cacgagcgcc ccgaccggct cacccggctc
     4081 cgcgacgggt acgagcgggc gctccccgcg ttcgggtcgg tgacgatcca ccggatccag
     4141 gtcggcgccc gtccgccggg ggacgtcgcc gccgagatcg cgcggctcac cgccgccggg
     4201 acgcgatgag cgggccgccg cgggccgcgc tcggcgcgga atgggacgcc cggctggaac
     4261 gcgtccgcct cgcgtcgcgc gcggtgcgcg gccacctgcc gcgcaccgcc gccgtcgccg
     4321 tcctgcgcgg gttcaccgcg cgcgagttcc tcggttcggc ggtcgccttc gccgccgggc
     4381 tgcctccgga ccggcgcgcc gcctggtacg gcagctacag caggacgatc ttcctcgcgg
     4441 gcgacccccg caacctcgcc gaacgccacc cctgcgacca cctttccgac gacggttcca
     4501 tagcctggta cgcccccgcc ccgatggcgg accgggaggg tctgcggcga ctgctcaggc
     4561 ccttccacgg gcccctcggc gtcaccggcc cggccgagga ggagatcccg gtcggcgaag
     4621 gcggcggggt cgcgcggctg gaggtcccgg tggcggacct gccggtcgag aactacctcg
     4681 tcaacgtcaa ccacctcctc gccgaggcgg ccatggacgg gctgttcacc gggatcgggc
     4741 ggctgctcgt ccggcatctt ccccgcgatc ccgatgagcg cgccatccgg tgggatcgca
     4801 tccgggtctc gcccgacgac cggtcggcgg gcacgttccg tgcccacgcc tacctggccc
     4861 tcagcccgta acgccagagg gatcccgcga ctctccgtat cgggcgtgtc cacctacgga
     4921 cgcggccttg ccggtcccgg cggcccggcg gaccatgggc gtgacgccgg gatcgtggcc
     4981 gcacccccga ggggagcacg atgcgggtag ttctcgtcaa catgccgtgg ggctccaccg
     5041 acgttccctc gctggccctg ggcatcctca aacgcgccgt ggcggaacgg atccccgaag
     5101 ccgaggtcga ggtgctgtac gccaacatcg acttcgtcga ctgggtccac gaccgcctcg
     5161 acatggcgat ggacgactac gacttcttcg cccagcggtc cttcaccgag gggaacggcg
     5221 actgggtctt ctcctcggcg ctctacgacg atccggactg gcggcggcgc gagttcgacg
     5281 agcggacgag cggggagaag gccgacctgg ccctgtgcca ccggctgcac gagctggcgc
     5341 ccgcgttcgt cgccgaactc gccggacgcg tcgccgcgtc cgcgcccgac gtggtcggct
     5401 tcacctccac cttccagcag aacgtcgccg cgctcgcggc ggccaggcag gtcaagcggc
     5461 tccggccgga gaccgtgacg atcctcggcg gggcgagctg cgacgggccg cagggcgccg
     5521 cgctgcaccg caacttcccg ttcgtggact tcgtcctgcg cggcgagggc gaggtcgcct
     5581 ttccgcgatt cctcaccgcc gtggcggggg cccgcgactt cgcggcgatt cccggcctcg
     5641 tctggcggga cgggagcgga gcctcgatcg ccaacgccat gagcgcgcgg ccgctggcgc
     5701 cgagtgagat cgtcgccccg gactacgacg actacttccg gcggtacgct acgtccgtcg
     5761 cgcggttgtg ggccgatccg cggctcgtcc tggaaagctc gcgcgggtgc tggtggggcg
     5821 agaagcacca ttgcggcttc tgcggtctga acgggtcgtt catggaattc cgcagcaagc
     5881 ccccggaccg gttcctggac gagatgctcg ccctggcccg gcggcaccag gtcctcgacg
     5941 tgtacatggt cgacaacatc ctcgacatgg gctatctgac gtcgatgctg cccgcgctcg
     6001 ccgccgaggg ctacgacttc cgcatgttct acgaggtcaa gtccaacctg cggcggcagc
     6061 aggtgcgggc gctggcggac gcgggcgtcg tgcgggtcca ggcgggcatc gagagcctca
     6121 gcggccgcgt cctcggcatc atggacaagg gcgtgaccgg ctgccagaac gtccggatga
     6181 tgcgcgacgc agaatcggcc ggcgtctggg tggagtggaa ctacctctac ggcttcccgg
     6241 gcgaggacgc cggcgactac acgtcggtcg tcgagcagtt ccccgcgctg caccacctgc
     6301 cgcccccggg catcgtcggc aggctcgcga tcgagcggtt cagcccgtac ttcgaccggc
     6361 ccgagttggg cttcgcggaa ctgcggcccc atccccagta ctcgatgatc tacgacctgc
     6421 ccgaagcgga gctgttcgac ctcgcctacc tcttcgaggc gccgccgcgc ggaatcgggg
     6481 cggcggtcgc cggacgcgtc gccgacgcgg ccgccgaatg gcaggcggcc tatcccgaga
     6541 gccggctgac catgtgcgac ctcggcgacc acatcgacct ggtgaaccgg cgcccggcct
     6601 tcggctggac cgcgctgcgg gtcgaggatc ccgtggagat ggccgccctg cgcctgctcg
     6661 accggccgca ccgcccggcg gcgctggccg ggaagctcgc cgcggtggac ccggccgtca
     6721 ccgtggacgg agtcgcggac ctgctcgcgc gctggcgcgg cctcgggctg accttcgccg
     6781 acggcggcca ggtcgtcctg accgccgccg acgcggcggg ccagggccgg cggcgggtgc
     6841 gccggcgcgc cgccgacccg gccgacctgg tcggagcgcc gtgatggccc cggtcacgat
     6901 gacgctgtgg cgcgactacg aggaactcga ccggccgggg gtgtgcgccg ggaccgccgc
     6961 cgtctccggc cccgccgccg aggcgagccg agccctcttc gaggacggcg tgcgccgcgt
     7021 cgcgctgaag gacaccgtgg acctttcggg cgcgaccgac gccgtcccca gcctcgtact
     7081 ggtaagggag ctgaccgcat acgggatcgc cgtcgactgg cgggtgcgcc tgggcgacgg
     7141 ccggtggcgg gacctgagcc acctctaccc gcccggcgag gtcgtcggcg acgaggaggc
     7201 ccgccgcgac tggcgggacg gctaccactt cttcaagtgc gtctaccgcc gcgggcccgg
     7261 attcctccag gtgcgcgacc ggcgttcggg cgtcctgcgc aagatcacca tcgacggccc
     7321 ggcgcatcgc gcggcggtcg agagcctgct ggagggctgc ccgtccgcgg cgctcccgcc
     7381 cccggtcctg cgcgacctga ggacgatgcg gctcgtccac ttcctcgacg gcgcggcctg
     7441 gtggctgccc tgccgggtgc gccggtggcc cgccggaaag agcgtcctgt gatcagtgga
     7501 agtgtccgga gagatgaggt gcggcatgac cgtgaagaga cccgaggaag cggggcgcga
     7561 gagcgcccgt gaggaggcgc tgcgcgccgc gctggcggag atgagcacgg agaacctggc
     7621 cacgaccgag ggcatccgcg tggacgtcgt ccgcgacgac atcgacgccc cgcacgagcg
     7681 cgactggggc atcggctgac cggagcggcc gacgggggtc ggccgaccgc gggcgttccg
     7741 cccaccccgg gtgccgcccg ccgggcggca cccggggccc ccgcctgccg gaggtgatga
     7801 cgatgacggc cgggacgccc gacgccgccg tgtgggacgg cgtgtccacc ctgctcgacc
     7861 actacatccg gatccggccc gacgaccggg tcgtcctcct gtacacggcg gacgcgcggg
     7921 accccgccgc gtgggtcgcc gccgagctgg ccgtccgggg cgtcaggccg gcgctgctcg
     7981 ggatgagcgc gggccgcgac gacaccgtgg agccccggct gcgcgcggcg ctccccgacc
     8041 ccgcgtcccc gcatccgcgg atcgtgctgc tgacgatgga gcgcgactcg atgtcgcacg
     8101 tgctccagct gcgccgggcc ctcgccccct accccgagga ccgctgggcg acctaccggg
     8161 tcatcaacgc ctcggcggag ttcttccgcc actcgatggg cgtgacgccg gagctgctgt
     8221 cggcgctgaa cggcgcgctg ctgaaccgga tgatgcccgc ctcccgcctg cgggtcacct
     8281 ccggcggggg caccgacctg gagatcgccg tcgactccga gcggtaccgg tggatcagca
     8341 accggggcgt gtggcgggcg ggggcgttcc tcgtcctgcc gccaggggag gtcagcacct
     8401 acccggcgga cgtcaacggc gtcctcgtcg ccgacggcgc gttcaacgtg acctcctaca
     8461 ccggccacga cgtccggctg gccgaccacc cggtcacggt cgagatcgag cacggccggg
     8521 tcaccggcgc cgcgtgcgcc gacccgctcg tggcgcggct gctggagcgc tgcctgcggg
     8581 tcgagaacgc cgacctgatc ggggagctgg gcttcggcac caacgtcggc accaccgcgt
     8641 tcatcccgct gaactcccac atcaacgagc gccacccggg cgtgcacctc gggctcggca
     8701 tgcacgggca gcgcctcgac gtggtcgact actggtgcga cgtccacctc gacctcatcg
     8761 ccgcggacgc gacggtcgcc gtcgacggcc gcaccgagat caggagcgcg gacctgcgcg
     8821 gtttcgccgg ggagcggcac cccgagcccc tgaccgggaa cgtccacgag gaggacatcg
     8881 acggtgactg ctgcggcctc atcaccggcg aggtcgcccg tgccagggag gagttcctcc
     8941 ggacgcggga gtgccgccga acccgggcta ccggcggctc gtgacgaagt cccggatcgt
     9001 ggtgatcatg tagtcgacca tctcctcggt caggcccggg aagacgccga tccagaacgt
     9061 gcgctcggtg atggcgtcgc tgttgtcgag cttccccgag acccggtacg gcctgccctg
     9121 gtaggcgggc tggcgggtga ggttgcccgc gaacagcatc cgggtgccga tccggcggtc
     9181 ctccaggtgg ttcacgatct cgtcccggtc gaacggcgcg tcggcggtga ccgtgatcac
     9241 gaacccgaac cagctcgggt cgctgcccgg cgtcgggacg ggcaggagca ggccgggcag
     9301 cccgtccagg ccctcgcgca gccgcgccca gttgcggcgg cgggccgcgc cgaactccgg
     9361 cagccgctgg agctgggaga gcccgagcgc cgcctggagg tcggtggtct tcaggttgta
     9421 gcccacgtgc gagaacgtgt acttgtggtc gtagccgtag ggcagctcgc cgaaccgctg
     9481 ggtgtagcgc ttgccgcagg tgtcgcagtc gccgggctcg caccagcagt cgcgtcccca
     9541 gtcgcgcagc gactcgacga tccgcgcgag ccgcggccgg tcggtggtga cgcagccgcc
     9601 ctcgcccatc gtcaggtggt gggcggggta gaagctcacg gtgctgaagt ggccgaacgt
     9661 cccggtcagc cgcccctggt agcccgcgcc gacaccgtcg cagttgtcct cgacgaagaa
     9721 cagcccgcgc tcggcggcca gctcggcgac ctcggccacc gggaacgggt tgccgagcgc
     9781 gtgcgcgatc atgatgccgc gggtgcgcgg gccgatcgcc tcggcgacgc ggtcggcggt
     9841 ggtgttgtag gtgccgagct ccacgtccac gaagaccggc gtcatctggt tctgcaggat
     9901 cgggttgacc gtcgtcggga agcccgccgc gaccgtcacg atctcatcgc ccggcctgag
     9961 ccggtcgtcg ccgatctccg gctgggtgag cgcggtcagc gccagcaggt tcgccgacga
    10021 ccccgagttc gtcaggtgca ccttgcggcg ccgcagcgtc cgcccgagct cgcgctccag
    10081 cgcgaccgcg ctcggcccgg acgcgatgcg caggttgagc gcggcctcga ccagcgcgac
    10141 gcggtcgtcc tcgctcaggt cggcgccgga cggcatcacc agcgtctcgc cggggacgaa
    10201 ccgccgcgcc tcctccttct cgcggtggta cttgcggacc agttccagga tcgaatcctg
    10261 ggagtcactc atcatcgcct ccaggacgcg ccacggcgcg gacctggtcc atcaaaacag
    10321 cggggcgcgg gatctcctcc cctgtaggcc ccccttagtg actcagctct cggcggggcg
    10381 cccgacgtcc gcccacaggc tcggcaggtt gaccacgatg ccctcctgcg tgctgcgcgc
    10441 gatgaccacg accgcctcct cgtcggggga ggggttctcc tcgcggtgcg ggacgtaggg
    10501 cgggacgaag atgtagtcgc ccggcgcggt ctccacccgg acctcgtcct cgccgtcggc
    10561 gaagacgaac accgggtggc cggagacgat gtagatcgcg gtctcggcct cgccgtggtg
    10621 gtggtcgccc gagctggtcg ccgggccgac gtgggtgcgg cccatccaga gccgctccga
    10681 gccgacgctc ttgcccgaga tcgcctcgta ccggcgcatc ccggaggtct ggacggtgtc
    10741 ggagccgagg tcggagccgc gcacgtgctc gatgcgcccg tgccaggccg ccgttccccg
    10801 tgcgcgcgcg tccgtcacga tccgcccctt ttgtcgttgt ttctggtcgg ttcagtagag
    10861 attggggccg ctgacggggc gccggtcgtc gtgccgcgcg gcctcgttgt cgtcgtaggc
    10921 ggcgcgcgcc gccgcgatct tcgcgtggtt gttctccgcc cacgccgaca gcgcgcgggt
    10981 ggaggcgaac agctcctcgg cgagcgccgt cgcggtgtac tcgacctgcg gcgggacggt
    11041 cgggtggacg gtgcgggtga tcagcccgtc ccgttcgagg gtgcgcaggg tgagcgtcag
    11101 catgcggcgg ctgatgcccg ggatcgaccg gtgcagttcg gcgaaccgga tcgggccgtc
    11161 gccggccgcc gcgatgatcg cgattcccca cttgctcccg acccggtcga gggcgtcgcg
    11221 gaaggccgcg cacgcgtcgg gctcgacgtc gtcgggtagc cgagatcgtg ctgccatcga
    11281 actcccctcc cggtgcggac gtctcagcct tacacgtgat cgtccgcacc ggcaacctgt
    11341 cccgcgcggg tcagggcggc gggtcgggga gcgaggcgcg cagcgtcggc gccacctcgg
    11401 tggcgaacag ttcgagggac gtccagtggt cgtccagcgg cagcccgtcg gggtcgacgc
    11461 tgaacacggc gagcgagtgg ccgaagatct cgtgctgccg gtgcaccttg tcgatgacct
    11521 gctccgggct gccgacgagc gccggcgagc gcgcgacgaa gtcctccacg ctcgcgtagg
    11581 gcggccgccc gccgatgctc cgctgcatcg cggcgaggcg ctcgaaggcg gggcggtagg
    11641 cggcgagcgc cgcctgggag gtcttggcga cgaggaaccc gggcgtcccc gcgccggtga
    11701 agagccggtc ggcgggtcgg ccgcgccgcg cccactcggc gcggtagtac tcgaccagcg
    11761 gccgcgcgct ctcgctggac aggcccgcgt tggagaacac cacggggtcg ccgtactcgg
    11821 cggccagcgc cagcgactcc gccgaggagg agctgccgtg ccagatccgg gggcgcccgt
    11881 tcag
//
