LOCUS       NZ_JAHFZY010000003     10396 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Actinomadura sp. NEAU-AAG7 Scaffold3, whole genome shotgun sequence.
ACCESSION   NZ_JAHFZY010000003
VERSION     NZ_JAHFZY010000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura sp. NEAU-AAG7
  ORGANISM  Actinomadura sp. NEAU-AAG7
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 977316)
  AUTHORS   Xu,X. and Zhao,J.
  TITLE     Actinomadura oryzae sp. nov., a novel actinobacterium with the
            activity of releasig potassium isolated from rhizosphere soil of
            rice in Sanya
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 977316)
  AUTHORS   Xu,X. and Zhao,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2021) College of Life Sciences, Northeast
            Agricultural University, 600 Changjiang Road, Xiangfang District,
            Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018603865.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 03:10:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,952
            CDSs (total)                      :: 7,871
            Genes (coding)                    :: 7,813
            CDSs (with protein)               :: 7,813
            Genes (RNA)                       :: 81
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 6 of 58
            Pseudo Genes (incomplete)         :: 53 of 58
            Pseudo Genes (internal stop)      :: 1 of 58
            Pseudo Genes (multiple problems)  :: 2 of 58
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:01:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 648106
            Orig. end                         :: 658502
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHFZY010000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10396
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="4"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10396
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10396
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5396](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5396)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            160..813
                     /locus_tag="KLI87_RS17590"
                     /old_locus_tag="KLI87_17595"
     CDS             160..813
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621467.1"
                     /locus_tag="KLI87_RS17590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_17595"
                     /product="HD domain-containing protein"
                     /protein_id="WP_156218382.1"
                     /transl_table=11
                     /translation="MGDTFAEFAMPDTDLTRAAYDLAFTAEQRALAHHSVRVYLFGRAL
                     GEARGLRPGDAYDDELLFVACLLHDMGLTDEGDGDQRFEVDGADVAAGFLDGQGVAPDR
                     VRVVWEAIALHTSVGIPQRMRPEIALTSAGAGLDVAGLHADRLPDGYADRVHAAYPRLK
                     KGSGLTHLVVDQIVRKPAKAPFGSLPYEIARQSGVDLKVPSWAEQLERAWSGVA"
     gene            complement(923..1744)
                     /gene="ehuA"
                     /locus_tag="KLI87_RS17595"
                     /old_locus_tag="KLI87_17600"
     CDS             complement(923..1744)
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657168.1"
                     /locus_tag="KLI87_RS17595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_17600"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_156218381.1"
                     /transl_table=11
                     /translation="MPPTDTTPEKDAAPLRGSAPGIRFEKVIKRFGSNTVLRELDFTVA
                     PGERVTLIGPSGSGKTTILRLLMTLEKPDGGLIFVGDDPLWHMDRGGKRVPANQKHLHE
                     MRKRIGMVFQQFNLFPNMNVLRNLTEGPMRVLGVPRDEAVERAKGLLDLVGLADKIDAH
                     PSQLSGGQQQRVAIARALAMEPQVLLLDEVTSALDPELVVGVQDLLRDIARQSDLTLLC
                     VTHEMTFAKDISDRVLMFDQGQIVEEGPPERMFGEPSEQRTRDFLQAVLAT"
     gene            complement(1731..2384)
                     /gene="ehuD"
                     /locus_tag="KLI87_RS17600"
                     /old_locus_tag="KLI87_17605"
     CDS             complement(1731..2384)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630633.1"
                     /locus_tag="KLI87_RS17600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_17605"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_156218380.1"
                     /transl_table=11
                     /translation="MEWDWSYTGDIIPDLLRGLRYTVIATLLGYLIAVVLGLVWALLRR
                     TPNVVVRQVVRWVTEFIRCTPLIPQLFFAFYVLPTWGLTFGKLTVGVLMLGIHYSTYMA
                     EVYRAGIADVPRGQWEAAKALNLPRTRVWLDVVLPQAVRKVIPTLGNYLIAMFKDSPLL
                     LAIGVPELLTSARSVGARTFEYLEPMTMVGVLFIVVSVISSILLRRLERHYATN"
     gene            complement(2384..3103)
                     /gene="ehuC"
                     /locus_tag="KLI87_RS17605"
                     /old_locus_tag="KLI87_17610"
     CDS             complement(2384..3103)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017542307.1"
                     /locus_tag="KLI87_RS17605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_17610"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_214617887.1"
                     /transl_table=11
                     /translation="MSEVLDGYEQWLKGAWLTLQLTVYGSGVALVLALVFGLAGTSRHA
                     WIRVLNRVYVEFFRGTATLVLLFWFFYALPLVGIRLNSTMFAAVLALGLNIGAYGAEVV
                     RGSIKAVPRAQYEATIALNFTPLQRMRRVLLPQGFALMLPPFGNLLIELMKGTAIVSLV
                     GLTDLTTVSKNLQSSTGETVASFSLVLVIYFVIAQILQLFVRFAEARVDRMLGRRPSRE
                     PELGTLAAGAPGAGVAS"
     gene            complement(3108..4001)
                     /gene="ehuB"
                     /locus_tag="KLI87_RS17610"
                     /old_locus_tag="KLI87_17615"
     CDS             complement(3108..4001)
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630631.1"
                     /locus_tag="KLI87_RS17610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_17615"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_156218379.1"
                     /transl_table=11
                     /translation="MTSSRRDFLRSAVLLSAAVPLAAAGCSTTDPKEEKSGGGSLQKAK
                     DAGKITVGFANEAPYGYTDKSGKLTGEAPELARVVFKELGIDKVAGVQVDFSGLIAGLN
                     AKRFDLVAAGMFITPERCNSVAFSDPEYVAKSAFMVRKGNPKGLLKQDDPGKKGVKVGV
                     LEGAVEQGYAEKSGVKKGNIQVFADQASGFEGLKAGRIDCIWLTRISLADLLTKHEGEP
                     YEVTAPFTPVIDGKEQFGAGAFAFRKADSDLVTAFNGKLAELKQGNRLLPIIQPFGFTQ
                     AEIPTAADTAAKFCKG"
     misc_feature    4085..4098
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.02"
                     /tool="antismash"
     gene            complement(4096..5001)
                     /gene="thpD"
                     /locus_tag="KLI87_RS17615"
                     /old_locus_tag="KLI87_17620"
     CDS             complement(4096..5001)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630630.1"
                     /locus_tag="KLI87_RS17615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_17620"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_156218378.1"
                     /sec_met_domain="PhyH (E-value: 4.5e-36, bitscore: 120.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIIESHPTLDDSYPTRKAAEAALLYRQDPVVYGKPQDGPIDSAT
                     LDSFDANGYLSVEELLTPAEVEDYRAELRRMAADPAILADERTVTERGSDEVRSIFEVH
                     RISEVFAALVRDPRVVGRARQILGSDVYVHQSRVNYKPGFTGKDFYWHSDFETWHAEDG
                     MPRMRAVSISIALTENFVHNGGLMIMPGSHKTFVACVGETPSDHYKESLRGQEIGTPDP
                     GSLSILADKHGIELFTGPAGSATMFDCNCMHGSNGNITPFPRSNVFVVFNSVENTCVEP
                     FAAPAPRPSFIGARDFTPVV"
     gene            complement(5001..5396)
                     /locus_tag="KLI87_RS17620"
                     /old_locus_tag="KLI87_17625"
     CDS             complement(5001..5396)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567884.1"
                     /locus_tag="KLI87_RS17620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_17625"
                     /product="ectoine synthase"
                     /protein_id="WP_156218377.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.1e-52, bitscore:
                     171.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLEEIIDTERDVRAETWCSRRFVLAKEGVGFSLHETILYAG
                     TETRMWYANHIEAVYCIGGQGELVNEETGEKHRIEPGVMYLLDGHEHHTLRAHTDVRTV
                     CVFNPPCTGREVHDENGIYPLLKEEDA"
     misc_feature    complement(5391..5406)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.24"
                     /tool="antismash"
     gene            complement(5463..6725)
                     /gene="ectB"
                     /locus_tag="KLI87_RS17625"
                     /old_locus_tag="KLI87_17630"
     CDS             complement(5463..6725)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-357). E-value: 1.4e-74.
                     Score: 243.1. Matches aSDomain:
                     nrpspksdomains_KLI87_RS17625_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657162.1"
                     /locus_tag="KLI87_RS17625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_17630"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_156218376.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.4e-74, bitscore:
                     245.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVFARMESEVRGYCRGWPTVFTTAKGSHMTDENGRTYLDFFAGA
                     GTLNYGHNNPQLKRRLIDYLAGDAVVHGLDMYTTAKRDFLERFNEYVLAPRGLDYKVQF
                     PGPAGNHSVEAALKLARKYTGRETIVSFTNAFHGMTLGALAVTGNSMKRTGAGVPLGHG
                     VAMPYDNYLDGRTPDFLLFETMLDDSGSGLDKPAAVIVETIQGEGGINVATAEWLRGLQ
                     DLCRRHDILLIVDDVQMGCGRTGPFFSFEEAGITPDIVCLSKSLSGYGLPFAVTLMRRD
                     LDVWEPGEHNGTFRGFNPAFVTAVAALEEYWLGAGMEKQTLAKGQQVETALNAIALQHA
                     GAIDSVRGRGLAWGLVMSDADLAPKVCEEAFSRGLLMETSGPESEVVKLLPPLTTSEAD
                     LGRGLEIVAESVEAVRSAVAV"
     aSDomain        complement(5655..6662)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLI87_RS17625_Aminotran_3.1"
                     /evalue="1.40E-74"
                     /label="KLI87_RS17625_Aminotran_3.1"
                     /locus_tag="KLI87_RS17625"
                     /protein_end="357"
                     /protein_start="21"
                     /score="243.1"
                     /tool="antismash"
                     /translation="FTTAKGSHMTDENGRTYLDFFAGAGTLNYGHNNPQLKRRLIDYLA
                     GDAVVHGLDMYTTAKRDFLERFNEYVLAPRGLDYKVQFPGPAGNHSVEAALKLARKYTG
                     RETIVSFTNAFHGMTLGALAVTGNSMKRTGAGVPLGHGVAMPYDNYLDGRTPDFLLFET
                     MLDDSGSGLDKPAAVIVETIQGEGGINVATAEWLRGLQDLCRRHDILLIVDDVQMGCGR
                     TGPFFSFEEAGITPDIVCLSKSLSGYGLPFAVTLMRRDLDVWEPGEHNGTFRGFNPAFV
                     TAVAALEEYWLGAGMEKQTLAKGQQVETALNAIALQHAGAIDSVRGRGLAWGLVM"
     gene            complement(7003..7551)
                     /gene="ectA"
                     /locus_tag="KLI87_RS17630"
                     /old_locus_tag="KLI87_17635"
     CDS             complement(7003..7551)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657161.1"
                     /locus_tag="KLI87_RS17630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_17635"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_156218375.1"
                     /transl_table=11
                     /translation="MAAQPLESPTRRPERTETNADVQLQEPSLTDGPGLWRIANESHVL
                     DVNSPYSYTLWCRDFADTSVVARDGSALCGFITGYVRPARPDTFFVWQVAVDESYRGRG
                     LARRMLDQLAGRQVARGQRYLEATVTPDNAASTAMFSSFARDHGCDLDRSPLFGTEHFP
                     GRGHEPEVLFRIGPLPGVN"
     misc_feature    7661..7678
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.51"
                     /tool="antismash"
     misc_feature    7815..7830
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.4"
                     /tool="antismash"
     misc_feature    complement(8167..8181)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.76"
                     /tool="antismash"
     gene            complement(8203..9171)
                     /gene="pip"
                     /locus_tag="KLI87_RS17635"
                     /old_locus_tag="KLI87_17640"
     CDS             complement(8203..9171)
                     /EC_number="3.4.11.5"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004177 - aminopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (39-301). E-value:
                     8.2e-35. Score: 112.5. Matches aSDomain:
                     nrpspksdomains_KLI87_RS17635_Abhydrolase_1.1"
                     /codon_start=1
                     /gene="pip"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592659.1"
                     /locus_tag="KLI87_RS17635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_17640"
                     /product="prolyl aminopeptidase"
                     /protein_id="WP_214617888.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 4.3e-10, bitscore:
                     36.3, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 4.1e-31, bitscore:
                     104.2, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELRTLYPPIEPYDSGLLDVGDGDSVYWEVCGNPEGKPAVMVHGG
                     PGGGCSAAHRRQFDPDSYRIVLFDQRNCGRSLPHAKDPGVSLEANTTWNLVADMERLRG
                     HLGIERWLVFGGSWGSALSLAYAQTHPERVSELVLRGIFTLRPFELYWFYQEGASLLFP
                     DLWESYVEPIPEDEREDLIAAFHERLNSPDRDVRVAAAKAWSTWEGSTVTLRPDPELAA
                     GFAEPDYAVAFARIENHYFVNEGFFEPEQLIRDVDRIRHIPAVIVQGRYDVCTPAATAW
                     DLHRAWPEAEFHLVDDAGHAYSEPGILHRLIEATDRFADAR"
     aSDomain        complement(8269..9054)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KLI87_RS17635_Abhydrolase_1.1"
                     /evalue="8.20E-35"
                     /label="KLI87_RS17635_Abhydrolase_1.1"
                     /locus_tag="KLI87_RS17635"
                     /protein_end="301"
                     /protein_start="39"
                     /score="112.5"
                     /tool="antismash"
                     /translation="VMVHGGPGGGCSAAHRRQFDPDSYRIVLFDQRNCGRSLPHAKDPG
                     VSLEANTTWNLVADMERLRGHLGIERWLVFGGSWGSALSLAYAQTHPERVSELVLRGIF
                     TLRPFELYWFYQEGASLLFPDLWESYVEPIPEDEREDLIAAFHERLNSPDRDVRVAAAK
                     AWSTWEGSTVTLRPDPELAAGFAEPDYAVAFARIENHYFVNEGFFEPEQLIRDVDRIRH
                     IPAVIVQGRYDVCTPAATAWDLHRAWPEAEFHLVDDAGHA"
     misc_feature    complement(9667..9681)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.92"
                     /tool="antismash"
ORIGIN
        1 acctgcccgg ggaccgccag gtcgaacgcc accaccccgt cgagcgcgag caccgcgacc
       61 acatgcatgg ccagaacgta gcgcacaggg gcactcggga cactggccgg gtgccggaag
      121 cgccgggagg atcgtggcaa ccgatcagag gagacctcga tgggcgatac cttcgccgag
      181 ttcgcgatgc ccgacaccga cctcacccgc gcggcctacg acctggcctt caccgccgag
      241 cagcgtgccc tcgcccacca cagcgtgcgg gtctacctgt tcgggcgggc actgggcgag
      301 gcgcgggggc tgcgtcccgg cgacgcctac gacgacgagc tgctgttcgt ggcgtgcctc
      361 ctgcacgaca tggggctgac cgacgagggc gacggcgacc agcggttcga ggtggacggc
      421 gccgacgtcg ccgccgggtt cctcgacggc cagggcgtcg cgccggaccg ggtacgggtg
      481 gtatgggagg cgatcgccct gcacaccagc gtcggcatcc cgcagcggat gcgccccgag
      541 atcgcgctga ccagcgccgg ggccggcctc gacgtcgccg ggctgcacgc ggacaggctc
      601 ccggacggct acgccgaccg cgtgcacgcg gcgtacccgc ggctgaagaa gggcagcggg
      661 ctcacccacc tggtcgtcga ccagatcgtc cggaagccgg ccaaggcgcc cttcgggtcg
      721 ctgccctacg agatcgcgcg gcagagcgga gtggacctca aggtcccgtc gtgggccgag
      781 cagcttgagc gggcgtggag cggcgtcgcc tgatccgcgt ctcccgcccc cggggacggg
      841 atcgcccgcg cccccggggc gacggggacg cgggcgatgc ttccgatggc ctggccggaa
      901 cggtcccgcc gggcgcggcc gctcaggtgg ccaggaccgc ctggaggaag tcccgggtgc
      961 gctgctcgga cggctcgccg aacatccgct ccggcgggcc ctcctcgacg atctgcccct
     1021 ggtcgaacat caggacccgg tcggagatgt ccttggcgaa ggtcatctcg tgggtgacgc
     1081 acagcagcgt gaggtcgctc tgtcgggcga tgtcgcgcag cagatcctgg acgccgacga
     1141 ccagctcggg gtcgagcgcg gaggtcacct cgtccagcag cagcacctgc ggctccatcg
     1201 ccagcgcccg cgcgatcgcg acccgctgct gctgcccgcc cgacagctgc gacgggtgcg
     1261 cgtcgatctt gtcggcgagc ccgaccaggt cgagcaggcc cttggcgcgc tcgacggcct
     1321 cgtccctcgg caccccgagc acgcgcatcg gcccctcggt gaggttgcgc agcacgttca
     1381 tgttcgggaa caggttgaac tgctggaaca ccatcccgat ccgcttgcgc atctcgtgca
     1441 ggtgcttctg gttcgccggg acgcgcttgc cgccccggtc catgtgccac agcgggtcgt
     1501 cgccgacgaa gatcaggccc ccgtcgggct tctccagcgt catcagcagc cgcaggatcg
     1561 tggtcttgcc cgacccgctc ggcccgatga gcgtcacccg ctcgcccggc gcgaccgtga
     1621 agtcgagctc gcggagcacg gtgttgctcc cgaaccgctt gatcaccttc tcgaagcgga
     1681 tgccgggggc gctgccccgc aggggcgccg cgtccttctc tggggtggtg tcagttggtg
     1741 gcatagtgcc tctccaggcg gcggaggaga atcgacgaga tgacgctgac cacgatgaac
     1801 agcacgccca ccatggtcat cggctccagg tactcgaacg tcctggcgcc caccgaccgc
     1861 gcggaggtga gcagctcggg gacgccgatc gccagcagca gcggcgagtc cttgaacatc
     1921 gcgatcaggt agttgccgag cgtcgggatc accttgcgga ccgcctgcgg caggacgacg
     1981 tccagccaca cccgcgtccg cggcaggttc agcgccttgg ccgcctccca ctgcccgcgc
     2041 gggacgtcgg cgatcccggc ccggtacacc tcggccatgt aggtcgagta gtggatcccg
     2101 agcatgagga cgccgacggt cagcttcccg aacgtcaggc cccaggtggg caggacgtag
     2161 aaggcgaaga acagctgggg gatcagcggg gtgcaccgga tgaactcggt gacccaccgc
     2221 accacctggc gcacgaccac gttcggcgtg cggcgcagca gcgcccacac caggccgagg
     2281 acgaccgcga tcaggtagcc gagcagcgtc gcgatgacgg tgtagcgcag gccccgcagc
     2341 aggtcgggga tgatgtcgcc ggtgtagctc cagtcccact ccatcagctc gccacccccg
     2401 ctccgggcgc gcccgccgcg agcgtgccga gttcgggctc acgcgagggg cgccggccga
     2461 gcatgcggtc gacgcgggcc tcggcgaagc ggacgaagag ctgcaggatc tgcgcgatga
     2521 cgaagtagat gacgaggacc agcgagaacg acgcgaccgt ctcgccggtg ctgctctgca
     2581 ggttcttgga gaccgtcgtc aggtcggtca gcccgaccag cgacacgatc gccgtgccct
     2641 tcatcagctc gatgagcagg ttgccgaagg gcggcagcat cagcgcgaac ccctggggga
     2701 gcaggacgcg ccgcatccgc tggagcggcg tgaagttcag cgcgatcgtc gcctcgtact
     2761 gcgctcgcgg caccgccttg atcgagccgc gcacgacctc ggcgccgtag gcgccgatgt
     2821 tcagcccgag cgcgagcacc gccgcgaaca tcgtggagtt cagcctgatg ccgaccagcg
     2881 gcagcgcgta gaagaaccag aacagcagca ccagcgtcgc ggtgccgcgg aagaactcca
     2941 cgtacacgcg gttcagcacg cggatccagg cgtgccgcga ggtgccggcc agcccgaaca
     3001 ccagggcgag cacgagtgcc acgccgctgc cgtagaccgt gagctggagg gtgagccacg
     3061 cgcccttcag ccactgctcg tagccgtcga ggacttcgga cacctgatca tcccttgcag
     3121 aacttggcgg cggtgtcggc ggccgtcggg atctccgcct gggtgaaccc gaacggctgg
     3181 atgatcggca gcagccggtt gccctgcttc agttcggcga gcttgccgtt gaaggcggtc
     3241 acgaggtcgc tgtcggcctt gcggaacgcg aacgcgcccg cgccgaactg ctccttgccg
     3301 tcgatgaccg gcgtgaaggg cgcggtcacc tcatacggct cgccctcgtg cttggtcagc
     3361 aggtcggcga gcgagatgcg ggtgagccag atgcagtcga tgcggccggc cttcaggccc
     3421 tcgaaaccgc tggcctggtc ggcgaacacc tggatgttcc ccttcttcac gccgctcttc
     3481 tcggcatagc cctgttcgac ggcgccttcc agcaccccca ccttcacgcc cttcttcccc
     3541 gggtcgtcct gcttgagcag gcccttgggg ttgcccttgc ggaccatgaa cgcgctcttg
     3601 gcgacgtact ccgggtcgga gaaggccacc gaattgcacc gctcgggtgt gatgaacatt
     3661 cccgccgcga cgaggtcgaa ccgtttggcg ttgagcccgg cgatgaggcc ggagaagtcg
     3721 acctggacgc cggccacctt gtcgatcccg agttccttga agaccacccg cgcgagttcg
     3781 ggcgcctccc cggtcagctt cccggacttg tcggtgtagc cgtagggcgc ctcgttggcg
     3841 aaaccgaccg tgatcttgcc ggcgtccttg gccttctgca gcgaaccgcc gcccgacttc
     3901 tcctccttgg ggtccgtcgt cgagcagccc gcggcggcca gcggcaccgc cgcggacagc
     3961 aggacggcgg aacgcaggaa gtcgcgtctc gaagaagtca tcgttgaact cctggatggt
     4021 gtgccgccgc gcccgcgggg ggccggatgc cccccgcggg cgcggggatg atcggtcggg
     4081 gttatcgaat tgttgtcaga cgaccggcgt gaagtcgcgg gccccgatga acgagggacg
     4141 cggcgcgggt gcggcgaacg gctcgacgca cgtgttctcg acgctgttga acaccacgaa
     4201 cacgttggag cgcgggaacg gggtgatgtt cccgttcgag ccgtgcatgc agttgcagtc
     4261 gaacatggtc gccgagcccg ccgggcccgt gaacagctcg atcccgtgct tgtcggcgag
     4321 gatggacagg ctgcccgggt cgggcgtgcc gatctcctgg ccgcgcaggg attccttgta
     4381 gtggtcggac ggggtctccc cgacgcaggc cacgaaggtc ttgtgcgaac caggcatgat
     4441 cattagcccg ccattgtgca cgaaattctc ggtgagcgcg atggaaatgc tcaccgcgcg
     4501 catgcgcggc atcccgtcct cggcgtgcca cgtctcgaag tccgagtgcc agtagaagtc
     4561 cttgccagtg aaccctggct tgtaattgac ccggctctgg tgcacgtaga cgtcggagcc
     4621 gaggatctgc cgggcccgcc cgaccacgcg cgggtcgcgg acgagcgccg cgaacacctc
     4681 gctgatccgg tggacctcga agatggagcg gacctcgtcc gagccgcgct cggtgacggt
     4741 gcgctcgtcg gcgaggatcg cggggtcggc ggccatccgg cgcagctcgg cgcggtagtc
     4801 ctcgacctcg gcgggcgtca gcagctcctc gacgctgagg tagccgttgg cgtcgaagga
     4861 gtcgagcgtg gcggagtcga tgggaccgtc ctgcggcttc ccgtacacga cgggatcctg
     4921 gcggtacagc agggcggcct cggcggcctt gcgggtgggg taggagtcgt cgagcgtggg
     4981 atgggactcg atgatgctca tcaggcgtcc tcctccttga gcagcgggta gatgccgttc
     5041 tcgtcgtgga cctcccggcc cgtgcagggc gggttgaaga cgcagacggt ccgcacgtcg
     5101 gtgtgggcac gcagggtgtg gtgctcgtgc ccgtccagca ggtacatcac gccgggctcg
     5161 atgcggtgct tctcgccggt ctcctcgttg accagttccc cctgaccccc gatgcagtag
     5221 acggcctcga tgtggttcgc gtaccacatc cgcgtctcgg tgccggcgta caggatggtc
     5281 tcgtgcagcg agaagcccac gccctccttg gccaggacga agcggcggct gcaccaggtc
     5341 tcggcccgca cgtcgcgctc ggtgtcgatg atctcctcaa gggaacgtac gatcatgaat
     5401 ctcctctttc cgctgaagcc gtgacggtgt gtcgatgggg ccggggcccg tgcgggcgcg
     5461 gctcagaccg ccacggccga gcggacggcc tcgacggact cggcgacgat ctccaggccg
     5521 cggccgaggt cggcctcgct ggtggtgagc ggcggcagca gcttcaccac ctcgctctcc
     5581 ggcccggagg tctccatcag cagcccgcgg ctgaacgcct cctcgcacac cttgggcgcg
     5641 aggtcggcgt ccgacatgac caggccccag gcgaggcccc ggccgcggac ggagtcgatc
     5701 gccccggcgt gctggagcgc gatcgcgttc agcgccgtct cgacctgctg gcccttggcg
     5761 agcgtctgct tctccatccc ggcgcccagc cagtactcct ccagcgcggc gacggcggtc
     5821 acgaacgccg ggttgaagcc gcggaaggtg ccgttgtgct cgcccggctc ccacacgtcc
     5881 aggtcgcggc gcatcagcgt gaccgcgaac gggagcccgt agccgctgag cgacttggac
     5941 aggcagacga tgtcgggcgt gatcccggcc tcctcgaagc tgaagaacgg cccggtgcgc
     6001 ccgcagccca tctgcacgtc gtcgacgatc agcaggatgt cgtggcggcg gcacaggtcc
     6061 tggaggccgc gcagccactc ggcggtcgcg acgttgatgc cgccctcgcc ctggatcgtc
     6121 tcgacgatca cggcggcggg cttgtccagg ccgctgccgc tgtcgtccag catcgtctcg
     6181 aacagcagga agtcgggcgt gcggccgtcg aggtagttgt cgtagggcat cgccacgccg
     6241 tggccgagcg ggaccccggc gccggtgcgc ttcatcgagt tgcccgtgac ggccagcgcg
     6301 ccgagcgtca tcccgtggaa ggcgttggtg aagctcacga tggtttcgcg gccggtgtac
     6361 ttgcgcgcca gcttcagcgc ggcctccacc gagtggttgc ccgccgggcc ggggaactgg
     6421 accttgtagt ccaggccccg cggcgcgagc acgtactcgt tgaaccgttc caggaagtct
     6481 cgcttggccg tggtgtacat gtcgagcccg tgcaccaccg cgtcgccggc gaggtagtcg
     6541 atgagccggc gtttcagttg cgggttgttg tgcccgtagt tcagcgtgcc ggcgccggcg
     6601 aagaagtcga ggtaggtgcg tccgttctcg tcggtcatgt ggctgccctt ggcggtcgtg
     6661 aacaccgtgg gccatcctcg gcaatagccg cggacctcag attccatgcg ggcgaaaacg
     6721 tccatggtct gcctccggca ggtgtggatg ggagcggggt tgcgtgcgga cgaacggggc
     6781 gggtggacgg acatctcgtg tgggcgatcg tgtcgcgggc ggttcaagcc gtcgttctgg
     6841 tgcaagccgt cggtcggggg cgcggagtcg ggggcggtcg ggtcggggat gcgggctgat
     6901 cgggcgggcc gatcggtgcg ggcgatccgt ccgggtgatg ggccgtcggc gatcgccgcg
     6961 gcggtggccg cgcgggcgat gggccggtgc cgggacatcc gcctagttca cgccggggag
     7021 cgggccgatc cggaagagga cctcgggctc gtgccccctg ccgggaaagt gctcggtccc
     7081 gaacaggggg ctgcggtcca ggtcgcagcc gtgatcgcgg gcgaacgagg agaacatcgc
     7141 ggtggaggcg gcgttgtccg gcgtcacggt cgcctccagg taccgctggc cgcgggccac
     7201 ctggcggccc gcgagctggt ccagcatgcg gcgggcgagg ccccggccgc ggtaggactc
     7261 gtcgacggcc acctgccaga cgaagaacgt gtcgggacgg gccggccgca cgtagccggt
     7321 gatgaatccg cagagcgcgg atccgtcacg ggccaccacg gaggtgtcgg cgaagtcgcg
     7381 gcaccacagc gtgtagctgt agggcgagtt gacgtccagg acgtgggact cgttcgcgat
     7441 ccgccacagc ccggggccgt cggtgaggct cggctcctgg agttgcacgt ccgcgttcgt
     7501 ctcggtccgt tcggggcgtc gggtcggtga ttccaggggt tgcgctgcca tgtccaggca
     7561 acttagcgaa tcgcgccgcg tgatcgctcc ggaatgttat ctgcgagatg tttaggacgc
     7621 tcgcgcaatg ggtatggcag tacgcttgcc aatttcggac atatttcgac gatgttaacg
     7681 ccgtgccgcg gcgcgcattt ccccctgtta tccaggcggg actgccgaat gggcggacga
     7741 cccctcgtgc ggcccatctg cccgtcactc tagtaagtac tcgggttggg gggagctgaa
     7801 ttctgtatcg gttgtgtgag gtgaatcact gagattcgct gacaacgcgc cgctgtgtgg
     7861 gggcgtcgtc acgcgtctgc ggaggtcgga ccggtccccg ccacgctggg gcgggtgggg
     7921 atcaaggtat gaacccgggc atcccggcat gtctcacact caccttgcga cgagacgtgc
     7981 catctgtcca ggcaagggtt tggtggccgg agggtaaggc ttcggccggt ttttcggacg
     8041 tcccccggcg ccatcggggc cgcgcgggaa cgcgcggccc cgttccattc cggatccacc
     8101 gcccggcgcg ttgcggcggc cccgtaatcg aggcttgccg gaaccggtca ggtggcttcc
     8161 cggagatttt cagattgctt ccagccgcct gcgggcgagc attcagcggg cgtccgcgaa
     8221 acggtcggtc gcctcgatga gccggtgcag gatgcccggc tcgctatagg cgtggcccgc
     8281 gtcgtccacg aggtggaact ccgcctccgg ccacgcccgg tgcaggtccc acgccgtcgc
     8341 cgcgggcgtg cacacgtcgt agcggccctg cacgatcacg gccgggatgt ggcggatcct
     8401 gtccacgtcc cggatgagct gctccggctc gaagaacccc tcgttgacga agtagtggtt
     8461 ctcgatccgc gcgaacgcga ccgcgtagtc gggctcggcg aagcccgccg ccagctcggg
     8521 gtcgggccgc agcgtcaccg tcgacccctc ccacgtcgac cacgccttcg ccgccgccac
     8581 ccggacgtcg cggtccgggg agttcagccg ctcgtggaag gccgcgatga ggtcctcccg
     8641 ctcgtcctcg gggatcggtt cgacgtagct ctcccacagg tcggggaaca gcagcgacgc
     8701 gccctcctgg tagaaccagt acagctcgaa cggccgcagc gtgaagatgc cgcgcagcac
     8761 cagctcgctc acccgctccg gatgcgtctg cgcatacgcc agcgacagcg cgctgcccca
     8821 gctcccgccg aacaccagcc accgctcgat gcccaggtgc ccgcgcagcc gctccatgtc
     8881 ggcgacgagg ttccaggtgg tgttcgcctc cagcgacacg cccgggtcct tggcgtgcgg
     8941 caggctccgc ccgcagttgc gctggtcgaa cagcacgatc cggtacgagt ccgggtcgaa
     9001 ctggcggcgg tgcgccgcgc tgcacccgcc ccccgggccg ccgtgcacca tcaccgccgg
     9061 cttcccctcg gggttcccgc agacctccca gtagacgctg tcgccgtcgc cgacgtcgag
     9121 cagcccggag tcatacggct cgatcggcgg atacaaggtg cgaagttcca tggtgcgcga
     9181 ttgtcccacc agcgcttcct caccgcgtct tcgtcgcgtt cccgtgtcgc gggggtgtga
     9241 cacgtggcac cgtgggaccg ggccgaacgc gcaccgcgaa aaccgtgatc aggcgtcctg
     9301 cgtgcgcgaa cgtcccgcga gcatcaagaa tccaaacccc caaactgggc aaacgctctg
     9361 cgggagcgca ctagagtgcc tttcgtgagc gaagcgacga gccgtagggc gaggagtgaa
     9421 gcgagcacca tgtccatggc agccccgcga acgcggctcc gagccgccag gcgaggaaca
     9481 aagtgagtga gctgaacgga tcgggtggtt cgcgcgggat cccggacgcg ggctccccct
     9541 ggcccgcgat gggccgcgac cccgcccagc agccgataca gatgtccaag cccggcgagg
     9601 ccacccgtcc ggaggtggag cagcccgagg cccagccctg ggacatcgtc gagcggctcc
     9661 gccagatggc cgacctcatc ggcgacgccc tcgccgcggg cgagcgcaag gtcagcgaga
     9721 cgcagaccga gaccgccgcc caggtcgccg ccgtgcaggc ggagaaggag tcggcgcagc
     9781 gggacgccgc ggccgcgtgg ggtgaggtgc agcgtgcgcg cggtcaggcc gagcaggccg
     9841 accgccgcgc cgtggaggcc gagcgccgcg tcgccgagac gcagaccgag tccgccgcgc
     9901 agctcgccgc cgtgcaggcg gagaaggagt cggcgcagcg ggacgccgcg gccgcgtggg
     9961 gtgaggtgca gcgtgcgcgc ggtcaggccg agcaggccga ccgccgcgcc gtcgaggccg
    10021 accgcaaggc cgccgacgcc gagcgccgca ccgccgaggt ccagaacgag gccgtcaccc
    10081 agatcgccgc gctgcagacc gagaaggagg ccgcgcaacg cgacgcggcc gccgcctgga
    10141 accaggccca gcagggccgc acccaggccg accagctccg cggccaggcc gagcaggccc
    10201 gcatgcaggc cgaccaggcc cgcggccagg ccgagcaggc ccgcgcccag atggagcaac
    10261 tccaggcgca ggtcgagcag gccgagcgcc gtgtcgccga ggccgaggcg accgtccggc
    10321 aggcgatcca gcagcgcgac gcgatgagcg aggcccgcga cgacgccctg cgcgccgtcg
    10381 aggacgccgt cggcgg
//
