LOCUS       NZ_JAHFZY010000002     11317 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Actinomadura sp. NEAU-AAG7 Scaffold2, whole genome shotgun sequence.
ACCESSION   NZ_JAHFZY010000002
VERSION     NZ_JAHFZY010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura sp. NEAU-AAG7
  ORGANISM  Actinomadura sp. NEAU-AAG7
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 1150629)
  AUTHORS   Xu,X. and Zhao,J.
  TITLE     Actinomadura oryzae sp. nov., a novel actinobacterium with the
            activity of releasig potassium isolated from rhizosphere soil of
            rice in Sanya
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1150629)
  AUTHORS   Xu,X. and Zhao,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2021) College of Life Sciences, Northeast
            Agricultural University, 600 Changjiang Road, Xiangfang District,
            Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018603865.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 03:10:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,952
            CDSs (total)                      :: 7,871
            Genes (coding)                    :: 7,813
            CDSs (with protein)               :: 7,813
            Genes (RNA)                       :: 81
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 6 of 58
            Pseudo Genes (incomplete)         :: 53 of 58
            Pseudo Genes (internal stop)      :: 1 of 58
            Pseudo Genes (multiple problems)  :: 2 of 58
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:01:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 491716
            Orig. end                         :: 503033
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHFZY010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11317
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11317
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11317
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6317](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6317)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1054..1509
                     /locus_tag="KLI87_RS11640"
                     /old_locus_tag="KLI87_11645"
     CDS             1054..1509
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891852.1"
                     /locus_tag="KLI87_RS11640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_11645"
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_214616957.1"
                     /transl_table=11
                     /translation="MQSRMQNPAAVLTGAMRPIQELFKAAHSGGVDQQTLELVHLRASQ
                     INGCSPCVDSGVKSARKAGVGDDKLALVAAWREAPYFTDEERAALALAEAATRLADRPD
                     AVDDETWDAAATYYDEKQLAAIVLMIALTNLFNRLNATTRQIAGAWG"
     gene            1578..2060
                     /locus_tag="KLI87_RS11645"
                     /old_locus_tag="KLI87_11650"
     CDS             1578..2060
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542531.1"
                     /locus_tag="KLI87_RS11645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_11650"
                     /product="iron chaperone"
                     /protein_id="WP_156217839.1"
                     /transl_table=11
                     /translation="MSETPKAAKGDAATGKNFDGFSEDERAAMKEHAKELKKAARRSAS
                     EAKAREEGERDVREKIAEMPEADRVLAERLHEIIASVAPGLVPRTWYGMPAYAKDGKVL
                     CFFQSAQKFKSRYATLGFSDVAALDDGTLWPTTYALTALDAANEAVISDLVKRAVG"
     misc_feature    2212..2227
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: medium, score: 25.17"
                     /tool="antismash"
     misc_feature    complement(2213..2226)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.32"
                     /tool="antismash"
     gene            2311..2865
                     /locus_tag="KLI87_RS11650"
                     /old_locus_tag="KLI87_11655"
     CDS             2311..2865
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLI87_RS11650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLI87_11655"
                     /product="hypothetical protein"
                     /protein_id="WP_214616958.1"
                     /transl_table=11
                     /translation="MSFSPEVLPAAVKAITNSPSRQRPVVMITCGADGLPQVCVPDWGE
                     LRVVGDRHLRVSVWPTDPTAVNLDRDAPALLIVTALSDVYLVHATARRLPGAAMVWAHY
                     ELTITSARIADRGTTPRLVPDHDTPTEFPAQRLPRARDMLGTKVTALRAHRRDPGPLIR
                     PRTRTTPRLRPGAGRAGRPSG"
     gene            complement(2792..3907)
                     /locus_tag="KLI87_RS11655"
                     /old_locus_tag="KLI87_11660"
     CDS             complement(2792..3907)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLI87_RS11655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLI87_11660"
                     /product="hypothetical protein"
                     /protein_id="WP_214616959.1"
                     /transl_table=11
                     /translation="MRHGLIRVVASIGAVLAVASTGHAVAAPAGPGPGPVLVDEPFTRA
                     TADSRFIGYGAACLTGAARGNAPQEGANHALSGCRRDPAGPVPPGGAAPRGYLQLTDAH
                     PEVTGAVMFDSPVPARDGLEVTFEQWQYGNTTRAPADGISFFLTDGARSLHTPGAFGGS
                     LGYAQKLPGGRSDAHFVPGVSGGYLGLGFDVLGEYFGDGERRGNGCERRSPAGTEFRAP
                     APGPNIVTARGPGNGIDGYCLLDATTSNRSSTGPWTSSLPGRLHGDLRTMPARVTAKKA
                     ESLLEPVKRVVRVVVTPAPNPVVTVDIDFQDDEGFQRVLRFPAPQPVPAAYRFGFAAST
                     GPLTDVQLIRKVVLRAQRPAGDEESSESEDE"
     misc_feature    4021..4034
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.52"
                     /tool="antismash"
     misc_feature    4022..4036
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.86"
                     /tool="antismash"
     misc_feature    complement(4027..4036)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
     gene            complement(4057..5004)
                     /locus_tag="KLI87_RS11660"
                     /old_locus_tag="KLI87_11665"
     CDS             complement(4057..5004)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF019604.5"
                     /locus_tag="KLI87_RS11660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_11665"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_214616960.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 6.9e-23, bitscore:
                     77.3, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSHDVVVVGGGPAGLSAALVLARACRAVAVVDAGRPRNAASAHV
                     HGVLGLPDLSPQVLLEVGRAQVLAYGVDVLEGTARTARPRPEGGFAVTLSDGRVLAGTH
                     LLLATGTVDELPDIPGLAPRWGRDAVACPHCHGWELRGRAIAILGGGRADARRALMWRQ
                     WTPNLTLITAPDHPPAPSDLERLGARGITVLDGPVTEVIAAADRLCGVRLGDGTSVPIR
                     ALVVAPCFRARADLLDGLGLRPTELTVGGRVVGTVIATDGAGRTAVPGVWAAGDGASPL
                     ERVVAVTADGAAVASAINAALTDGSHPEHSAGEP"
     gene            complement(5001..6317)
                     /locus_tag="KLI87_RS11665"
                     /old_locus_tag="KLI87_11670"
     CDS             complement(5001..6317)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.5"
                     /locus_tag="KLI87_RS11665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_11670"
                     /product="YcaO-like family protein"
                     /protein_id="WP_214616961.1"
                     /sec_met_domain="YcaO (E-value: 6.4e-38, bitscore: 127.2,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAQDRYQRSGPLPREGALWCADHFRGARGFRAAAPHAGERAFPL
                     PRARALIEAALDEAALRPRLTDLRGAWRCHLSHPDGTPVRDGVGAGKGDPQSAQVGALY
                     EALEHHRGAALPARGEPVELLTAAQLAGTDLARDPALEPLAASGGPGAAACRRYTALDH
                     SGRTLPVPVFLTNPAYLECPADRRHELGDVLDYAPLSRFSTNSGTAIGATRDEALVHAL
                     AEAVERDAFSLLLAGTFLLARPSPLRLVRPASLPDHLQELYLSAGWHAGGSVALIDMTS
                     DLGVPAFCAHTSRDGRAAQGQGASLSAEHAVRRALNELIQVVAIRRGERNPPADLRAVE
                     SHPRLLEAARADFDPHLAGARPRDFLPTGAPAAPREHLQRLTATLTGYGYRPYGRVLRT
                     EPNGVSTVSVFVPGLERFFAVTAGACVVPGPRGRALGPG"
     misc_feature    6446..6459
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.82"
                     /tool="antismash"
     misc_feature    complement(6447..6460)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.95"
                     /tool="antismash"
     gene            complement(6547..7077)
                     /locus_tag="KLI87_RS11670"
                     /old_locus_tag="KLI87_11675"
     CDS             complement(6547..7077)
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012859.5"
                     /locus_tag="KLI87_RS11670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_11675"
                     /product="RICIN domain-containing protein"
                     /protein_id="WP_214616962.1"
                     /transl_table=11
                     /translation="MKRTTRVLGAALALLFVGVGNGTAFAQPAQPAQPAKTTRTAKAAD
                     AKILAVRHFYNDRFQRCLDADTNTGGNGGIVQLWDCGPWNQQDWEVWPDGHIYNSRFQK
                     CLDADTNTGGNGGKVQLWGCGPWNQQWWDIWPDGHLYNRRFQRCLDADTNTGGNGGIVQ
                     LWDCGPWNQQWWR"
     gene            complement(7221..7823)
                     /locus_tag="KLI87_RS11675"
                     /old_locus_tag="KLI87_11680"
     CDS             complement(7221..7823)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540668.1"
                     /locus_tag="KLI87_RS11675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_11680"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_308165975.1"
                     /transl_table=11
                     /translation="MSPRHSIAEAARTRDRIVRAAVRDASRLGLEGLTVGSLARRLGMS
                     KAGLIGPFGSREALLAAALDHAVQIFQAAVIAPLEQRPPGAERLELLIDRWVAYLADCP
                     FPGGCFVTAASSELDGRPGPLRDRLREGVASWRARIAAEVAAAQAELPGPHRPAEEVAV
                     TLVGLSMALNQEVQLLEDREAAPRARAAMRHAAGLRP"
     gene            7895..8452
                     /locus_tag="KLI87_RS11680"
                     /old_locus_tag="KLI87_11685"
     CDS             7895..8452
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /locus_tag="KLI87_RS11680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_11685"
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="WP_156217833.1"
                     /transl_table=11
                     /translation="MTDLFRLDATALALIGHDVALIPDAAMADPTPCEGWTVADLLHHM
                     NERHEALARTVLPPLEPGEDPRDDFARVAGRSLTALDRTGATVDLPWRGPAPTEWVLGV
                     HVVDMLVHRWDLARALGTALDTPAGLLRAALPLARANTAPGSVLNGPGGVYLPPLPEDS
                     GRSAIDTIVALLGRDPRWTPPA"
     gene            complement(8501..9157)
                     /locus_tag="KLI87_RS11685"
                     /old_locus_tag="KLI87_11690"
     CDS             complement(8501..9157)
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012301.5"
                     /locus_tag="KLI87_RS11685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_11690"
                     /product="response regulator transcription factor"
                     /protein_id="WP_214616963.1"
                     /transl_table=11
                     /translation="MSVPVLIIEDHPLYLGALRALFERGDGFEVVAEAATGAAALEALE
                     GPRGARPRVITLDLHLPDGSGTALAQRVLAAHPQVRVLVISALTDRAAVLSALRAGVHG
                     YLTKDAGSEEIRHAVRLVAAGGTAFGEPIAVLLRDWLKADAGASARTHFPTLTGRETQV
                     LDLLAGGREYAQIARELGISEKTVRNHLSNLFVKLEVNNRAQAILVARRAGMGTS"
     gene            complement(9154..9819)
                     /locus_tag="KLI87_RS11690"
                     /old_locus_tag="KLI87_11695"
     CDS             complement(9154..9819)
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019350.5"
                     /locus_tag="KLI87_RS11690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_11695"
                     /product="sensor histidine kinase"
                     /protein_id="WP_214616964.1"
                     /transl_table=11
                     /translation="MDRREKNGKVAWELTAGIEEERQRLRREIHDQMAPALAALRLRFD
                     LAREVVADGAGGPDPLDSLDSVDEAIRNLGDALSGLLAAIDGAFPAELARLGLIGALAD
                     LGAFLSLADLRIEADENELLEMSPACEVAAYKIAAEALTNARKYSSDRSAVVRIDRQDG
                     DIVLQVSSAIGSERERHPSYGVGFRSMRERAAALGGKCNVRATDEGMVVRARIPSRPA"
     gene            complement(9888..10232)
                     /locus_tag="KLI87_RS11695"
                     /old_locus_tag="KLI87_11700"
     CDS             complement(9888..10232)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLI87_RS11695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLI87_11700"
                     /product="hypothetical protein"
                     /protein_id="WP_156217830.1"
                     /transl_table=11
                     /translation="MDSDVRVAVCGAGLRVWFPWPARACSVVDVRGLPARASVGKGLTI
                     GLDGLPENVMGLLARVETRAAEIDVAGAALRVHVPGGHAEFQMEGESTVALRLCGRELV
                     GIDVRFLGRP"
     misc_feature    10359..10374
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.89"
                     /tool="antismash"
     gene            complement(10400..11068)
                     /locus_tag="KLI87_RS11700"
                     /old_locus_tag="KLI87_11705"
     CDS             complement(10400..11068)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290588.1"
                     /locus_tag="KLI87_RS11700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_11705"
                     /product="HD family hydrolase"
                     /protein_id="WP_214616965.1"
                     /transl_table=11
                     /translation="MTSTTRRDLIGRAEPPRDVPERLASQIAFIAEVDRLKTVVRRSPL
                     LAADRRENDAEHSWHLALMVTVLAEYADEPIDVARTLRLVLVHDLVEIYAGDTFLYDEA
                     ASADQAEREEAAADELFALLPPDQAGAFRALWDEFEARATPEARFAKAMDRLQPLLLNF
                     GNEGGTWHTPGVTEAGVRRRKSVIGDASAELGAYASKLIDLGAQRGWLRRDGGGDGVGS
                     "
ORIGIN
        1 gtcgccgctg gcgcgggcca gcagctcggc gctctcgcgc ggggagagca cgtccagcga
       61 caccgggggc acctcgtcca ggcccagcag gcggttgcgg ctggtgatca gcgcgaccga
      121 cggcccggcg cccggcagca gcggccggac ctgctcggcg cccgcggcgt tgtcgagcac
      181 cacgacggcc cgccggtgcg ccagctccga gcgccagcat gcggccagcg gctcgatgcc
      241 gccctcctgc gggatcttct cggacgggac gtcgagggcg gcgagcagcg tccgcagcgc
      301 ggcgtcgggg tcgagcggct cgcgcccggc ggtgaacccg tgcaggtcga catagagctg
      361 ggcgtcgggg taggcggcgg cgagccggtg cgcggcgcgc accgcgaggc aggtcttgcc
      421 gacgcccgcc atgccgtcga gcgcgacgac ccggtcggcc tccacggcgg cgaggacggc
      481 ggccagctcg gcctcgcgcc cggtgaagtc gggcaggtcg cgcggcaggt cgttgcgggg
      541 cgggcgcggc gccccggcgg gcggcgacgg cggctggacc tggcggctcg tcttcggcgg
      601 cgcgggcagc gggttcgccg cgcgctccca gagcgggcgc agcgggcgcg ggtcgcgctg
      661 gacgaggcgg cacagcgcga tcacggcggg ccagggcggc acggtctggc cgccgaggta
      721 gcgcgacagc gacgacgagc tgaggccggc gtcgcgcgcg agggcgcgga ctccgcgctc
      781 ggacacctcc tggagcaggc gcagccgggc ggccagctcg tcccgggggt ccgcgcgcgg
      841 gccggcctgc ggtgtccggt gttccacggt catccgcccg gtgcctccct gtcccatgcc
      901 ggtacaactc ttctggatag attagccctg ctcaggggtg tttcgactgt cccacggtgt
      961 cccacggcca tcggcatgcg cggcccgtcc cgctgtgatg gagccatgcc ccgagggaac
     1021 ggggcggccg atcacacgaa ggagaacacc gagatgcagt cccggatgca gaaccccgcc
     1081 gccgtcctga ccggtgcgat gcggccgatc caggagctgt tcaaggccgc gcactccggc
     1141 ggggtggacc agcagacgct ggagctggtg cacctgcggg ccagccagat caacgggtgc
     1201 agcccgtgcg tcgacagcgg cgtcaagtcg gcccgcaagg ccggcgtggg cgacgacaag
     1261 ctggccctgg tcgccgcctg gcgcgaggcc ccctacttca ccgacgagga gcgggcggcg
     1321 ctggcgctgg ccgaggccgc gacgcggctg gccgaccgcc cggacgcggt ggacgacgag
     1381 acctgggacg ccgccgccac ctactacgac gagaagcagc tcgccgcgat cgtcctcatg
     1441 atcgcgctca ccaacctgtt caaccggctc aacgccacca cccggcagat cgccggggcc
     1501 tggggataac cagaccagaa ggacggccct ccgccgaggg cccgccagcg agaaaccgag
     1561 agaaggggat tcccatcatg agcgagacac cgaaggccgc caagggcgac gccgcgaccg
     1621 gcaagaactt cgacgggttc tccgaggacg agcgggccgc gatgaaggag cacgccaagg
     1681 agctgaagaa ggccgcgcgg cgcagcgcgt ccgaggccaa ggccagggag gagggggagc
     1741 gcgacgtccg cgagaagatc gccgagatgc cggaggccga ccgggtgctc gccgagcggc
     1801 tgcacgagat catcgcctcg gtcgcgccgg gcctcgtccc gcggacctgg tacgggatgc
     1861 ccgcctacgc caaggacggc aaggtgctgt gcttcttcca gagcgcgcag aagttcaagt
     1921 cgcggtacgc gacgctcggc ttcagcgacg tggccgcgct cgacgacggc acgctgtggc
     1981 cgacgaccta cgcgctgacg gccctggacg ccgcgaacga ggccgtgatc agcgaccttg
     2041 tgaagcgggc ggtcggctga ccctcaatcg aacagccggg cccacgatcg gcgccgtttc
     2101 acaacggccc cggtagtggg cccggctgtg gtttgggcca tgcagcacca gggtaaacgg
     2161 gcgccccacg cgtggcgcca ctcgaaaaaa gccgtgtgat cgttagcact ctcgaatgta
     2221 tgatcgaaca atatcgggaa ggattcatag caagaccggc tcggccgcac gccgggccgg
     2281 cggaccttcg acgtaagggc ggacacaccg atgtcgttct cacccgaggt gctcccagcc
     2341 gccgtcaaag cgatcaccaa ctccccgtcc cggcaacgtc ccgtcgtcat gatcacctgt
     2401 ggcgccgacg ggctgcccca ggtctgcgtc ccggactggg gcgaactgcg cgtcgtcggc
     2461 gaccggcacc tgcgggtgtc ggtctggccg accgacccca ccgcggtcaa cctcgaccgc
     2521 gacgcgcccg cgctgctgat cgtcaccgcc ctgtccgacg tctacctcgt ccacgccacg
     2581 gctcgcaggc tgcccggcgc cgccatggtg tgggcccact acgaactcac gatcacctcg
     2641 gcgcggatcg ccgatcgggg caccaccccg cggctcgtcc ccgaccacga caccccgacc
     2701 gagttcccgg cccagcgcct gccgagggcg cgcgacatgc tcgggacgaa ggtcaccgcg
     2761 ctccgggccc accgccggga ccccggcccg ctcattcgtc ctcggactcg gacgactcct
     2821 cgtctccggc cgggcgctgg gcgcgcagga cgaccttccg gatgagctgc acatcggtga
     2881 gcggcccggt ggacgcggcg aacccgaacc ggtaggccgc cgggaccggc tgcggggccg
     2941 ggaaccgcag gacccgctgg aagccctcgt cgtcctggaa gtcgatgtcg accgtgacca
     3001 ccgggttcgg cgcgggcgtc acgacgacgc gaacgacccg cttcaccggc tccagcaggc
     3061 tctcggcctt cttcgcggtg accctggcgg gcatcgtcct caggtcgcca tgcaggcgtc
     3121 cgggcaggct cgacgtccag gggccggtgg acgacctgtt gctcgtcgtg gcgtcgagca
     3181 ggcagtagcc gtcgatgccg ttcccggggc cgcgcgcggt cacgatgttg ggcccgggag
     3241 cgggcgcgcg gaactccgtc ccggccgggg aacgccgctc gcagccgttc ccgcgccttt
     3301 ccccgtcgcc gaagtactcc cccagcacgt cgaatccgag gccgaggtat ccgccgctga
     3361 cgccggggac gaagtgcgcg tccgacctgc cgcccggcag tttctgcgca tatccgaggc
     3421 ttccgccgaa cgcgcccgga gtgtgcagcg accgcgcgcc gtcggtgagg aagaacgaga
     3481 tgccgtcggc gggcgcccgg gtggtgttgc cgtactgcca ctgctcgaag gtcacctcca
     3541 gcccgtcccg cgccgggacg ggcgagtcga acatcacggc gccggtcacc tcggggtggg
     3601 cgtcggtgag ctggaggtag ccgcgcggcg cggcgccgcc cggcggcacc ggaccggccg
     3661 gatcccggcg gcagcccgag agcgcgtggt tcgcgccctc ctgcggcgcg ttcccccgcg
     3721 cggcgcccgt caggcaggcg gcgccgtatc cgatgaagcg cgagtcggcg gtggcgcggg
     3781 tgaacggctc gtccaccagg accggccccg gccccgggcc cgcgggcgcg gccacggcgt
     3841 gcccggtcga cgcgacggcc agcacggccc cgatcgacgc gaccacgcga atcaacccat
     3901 gtctcatatc gtcgattctt tctgcgcgaa ttccccggtg atctccttct ccgaactcat
     3961 gggaccacga actcggggcg ggagcggccc attacctgtt ccatgggaat atgcgcggca
     4021 gatcattaag aaaacgcatg tcggcccatg cggaggtcac ggctccccag cggagtgctc
     4081 cgggtgcgac ccgtcggtga gggccgcgtt gatcgcggac gcgaccgccg cgccgtcggc
     4141 ggtgaccgcg acgacccgct ccagcgggga cgcgccgtcc ccggcggccc agacccccgg
     4201 cacggcggtg cgtccggcgc cgtcggtcgc gatgacggtg ccgacgaccc ggccgccgac
     4261 cgtcagctcg gtggggcgca ggccgaggcc gtccagcagg tcggcgcggg cccggaagca
     4321 cggggccacc accagggcgc ggatcgggac ggacgtcccg tcgcccagcc gcaccccgca
     4381 gagccggtcg gcggcggcga tcacctcggt gacggggccg tcgaggacgg tgatgccccg
     4441 cgcgcccagc cgttcgaggt cggacggggc gggcgggtgg tcgggagcgg tgatcagggt
     4501 gaggttcggg gtccactgcc gccacatcag ggcccggcgg gcgtccgcgc ggccaccgcc
     4561 caggatcgcg atcgcgcggc cgcgcagttc ccagccgtgg cagtgcgggc aggcgacggc
     4621 gtcgcgcccc cagcgcgggg ccaggccggg aatgtcgggc agctcgtcga cggtgccggt
     4681 ggcgagcagc agatgcgtcc cggcgaggac cctgccgtcg gacagggtga cggcgaaccc
     4741 gccctcggga cgcggccgcg ccgtgcgggc cgtcccctcc agcacgtcga cgccgtaggc
     4801 gaggacctgc gcgcggccca cctccagaag gacctgcggc gacaggtcgg gaagccccag
     4861 cacgccgtgg acgtgggcgg acgcggcgtt gcggggccgc ccggcgtcga cgacggccac
     4921 ggcgcggcag gcgcgggcca gcaccagcgc ggcgctgagc ccggcgggcc cgccgccgac
     4981 gacgaccacg tcgtgggagg tcatccgggg ccgagggcgc ggccgcgcgg gccggggacg
     5041 acgcaggcac cggcggtgac cgcgaagaag cgttccagac cgggcacgaa caccgacacc
     5101 gtgctcacgc cgttgggctc ggtgcgcagg acccggccgt aggggcggta cccgtagccg
     5161 gtgagcgtgg ccgtgaggcg ctggaggtgc tcgcggggcg cggcgggcgc gccggtcggg
     5221 aggaagtcgc gcgggcgggc accggccaga tgcgggtcga agtcggcgcg tgccgcctcc
     5281 agcaggcgcg ggtggctttc caccgcgcgg aggtccgccg gcgggttccg ctcgccgcgc
     5341 cggatcgcga cgacctggat cagctcgttc agcgcccgcc gcaccgcgtg ctcggccgac
     5401 agcgacgcgc cctgcccctg cgccgccctc ccgtcgcggg aggtgtgcgc gcagaacgcc
     5461 ggaacgccca ggtcgctggt catgtcgatc aacgcgaccg agcctccggc gtgccatccg
     5521 gcgctcaggt agagctcctg gaggtggtcg ggcaggctcg ccggacgcac cagccgcagc
     5581 ggcgacgggc gggcgagcag gaacgttccg gccagcagca aggagaaggc gtcacgttcc
     5641 accgcctcgg ccagggcgtg caccagggcc tcgtcgcggg tcgcgccgat cgcggtgccg
     5701 ctgttggtgc tgaaccggct cagcggggcg tagtcgagga cgtcccccag ctcgtggcgg
     5761 cggtcggccg ggcattccag gtaggcgggg ttggtgagga acaccggcac cgggagcgtg
     5821 cggccggagt ggtccagcgc ggtgtagcgc cggcacgccg ccgcccccgg cccgccggac
     5881 gcggcgagcg gctccagggc cgggtcccgc gccaggtcgg tgccggcgag ttgggcggcg
     5941 gtgagcagct cgaccggttc gccgcgcgcg gggagggcgg cgccgcggtg gtgctccagc
     6001 gcctcgtaca gggccccgac ctgcgcggac tgcgggtctc ccttccccgc gcccaccccg
     6061 tcgcggacgg gggtgccgtc gggatgggag aggtggcagc gccaggcgcc gcgaagatcg
     6121 gtcagccggg ggcgcagcgc cgcctcgtcc agcgccgcct cgatgagggc cctggcgcgc
     6181 ggcagcggga acgcgcgttc tccggcgtgc ggcgcggcgg cccggaaacc gcgggcgccg
     6241 cggaagtggt ccgcgcacca gagggccccc tcccggggga ggggaccgct ccgttggtac
     6301 ctgtcctgcg cggtcaactg gccgactcgt cctcggcctg aggccgctcc atccgctgga
     6361 ggcgttccgc ctcctcggcg gtcagctcgc cgacggcgat gttgggaccg cagcggtata
     6421 cctcacagcc cattgcactc gctccctaac tctgcgtgac tggcagtgtc gagaggtact
     6481 gtgccccacc gcgccgggcg ggggaacatc cggggcggat agttcccggt tgcctcgcgg
     6541 ccccgcttat cgccaccact gctggttcca gggaccgcag tcccacaact ggacgatgcc
     6601 gccgttgccg ccggtgttgg tgtcggcgtc caggcaccgc tggaaacgcc tgttgtagag
     6661 gtggccgtcg ggccagatgt cccaccattg ctggttccag gggccgcagc cccagagctg
     6721 gaccttgccg ccgttgcccc cggtgttggt gtcggcgtcc aggcacttct ggaagcgcga
     6781 gttgtagatg tggccgtccg gccagacctc ccagtcctgc tggttccagg ggccgcagtc
     6841 ccacagctgg acgatgccgc cgttgccgcc ggtgttggtg tcggcgtcca gacaacgctg
     6901 gaaccggtcg ttgtagaagt gccgtaccgc caggatcttc gcgtcggcgg ccttcgcggt
     6961 ccgggtggtc ttcgcgggct gtgcgggttg cgccggctgc gcgaacgccg tgccgttgcc
     7021 gacaccgacg aaaagcaacg ccagcgccgc ccccaacacg cgagtcgtcc gtttcatttc
     7081 gcttcccttg gtcggatgat gcggatccga atgacgcaca tcctgttccg gacgactccc
     7141 ccggcccatc cccaaatatt ggggggttac ggaccactcc ggcccgcgtt actctcgcgg
     7201 gtttcgcggt tctccggcgc tcagggacgc agccccgcgg catgccgcat ggccgcgcgg
     7261 gcgcgggggg cggcctcgcg gtcctccagc agctggacct cctggttgag ggccatcgag
     7321 aggccgacca gggtcacggc gacctcctcc gcgggacggt gcgggcccgg gagctcggcc
     7381 tgcgccgcgg cgacctcggc ggcgatccgc gcgcgccagg aggccacgcc ctcgcgcagc
     7441 cggtcgcgca gcggccccgg gcggccgtcg agttcggacg aggcggccgt cacgaagcag
     7501 ccgccgggga acgggcagtc ggccaggtag gccacccacc ggtcgatgag cagttccagc
     7561 cgttccgcgc cggggggccg ctgttccagc ggggcgatga ccgccgcctg gaagatctgc
     7621 acggcatggt cgagcgccgc cgccagcagc gcctcgcggg atccgaaggg gccgatcagg
     7681 cccgccttgc tcatgccgag ccggcgcgcg agcgagccga ccgtcagccc ctccaggccg
     7741 aggcgcgagg cgtcccgcac cgcggcccgc acgatccggt cgcgcgtccg ggccgcttcg
     7801 gcgatcgagt ggcgaggaga cacgggccca tcatagcgaa cgaccgtacg ttatagtttt
     7861 ggcgtacgat cgttcgctat taccgggagc ccccatgacc gacctgttcc gcctggacgc
     7921 cacggcgctc gccctgatcg ggcacgacgt ggcgctgatc cccgacgccg cgatggcgga
     7981 cccgaccccc tgcgagggat ggacggtggc ggatctgctc caccacatga acgagcggca
     8041 cgaggccctc gcccggacgg tgctgccgcc tctcgaaccg ggcgaggacc cccgggacga
     8101 cttcgcccgt gtcgccgggc gctcgctcac ggccctcgac cggacggggg cgaccgtgga
     8161 cctcccgtgg cggggcccgg cgccgaccga atgggtgctc ggcgtccacg tcgtcgacat
     8221 gctggtccac cgctgggacc tggcccgcgc gctgggcacc gcgctcgaca caccggccgg
     8281 gctgctccgg gccgcgctgc cgctcgcccg cgccaacacc gcccccggca gcgtcctcaa
     8341 cgggccgggc ggcgtctacc tcccgcccct ccccgaggac tccggccgtt ccgcgatcga
     8401 caccatcgtc gccctgctcg gccgcgaccc gcgctggacg ccgccggcgt gacccgcgaa
     8461 agcgctgacc gacgcgcggg cggccccgcc ggagggccgc tcaggaggtg cccatgcccg
     8521 cccggcgcgc caccaggatc gcctgcgccc ggttgttcac ctccagcttc acgaacaggt
     8581 tggagaggtg gttgcgcacc gtcttctccg agatccccag ctccctggcg atctgcgcgt
     8641 actcccggcc cccggcgagc aggtcgagca cctgcgtctc gcgtccggtg agcgtcggga
     8701 agtgcgtcct cgcggacgcg ccggcgtcgg ccttcagcca gtcgcgcagc agcacggcga
     8761 tcggctcgcc gaaggccgtc ccgccggccg cgaccagccg caccgcgtgc cggatctcct
     8821 cgctgccggc gtccttggtc aggtagccgt gcaccccggc gcgcagcgcc gacagcaccg
     8881 ccgcccggtc ggtcagcgcg ctgatgacca gcacccgcac ctggggatgg gcggccagga
     8941 cgcgctgcgc cagcgcggtg cccgagccgt ccgggagatg gaggtcgagg gtgatcacgc
     9001 gcggtcgcgc gccgcgcggg ccctccagcg cctccagcgc ggccgcgccg gtggcggcct
     9061 ccgcgaccac ctcgaatccg tcgccgcgct cgaagagcgc gcgcagcgcg ccgagataga
     9121 gcgggtggtc ctcgatgatg agaacgggaa cgctcatgcc ggccgcgacg ggatccgggc
     9181 ccggacgacc attccctcgt cggtcgcgcg gacgttgcat ttgccgccga gggcggcggc
     9241 ccgctcccgc atggagcgga atccgacgcc gtaggagggg tggcgctcgc gctcgctgcc
     9301 gatggcgctg ctgacctgga ggacgatgtc cccgtcctgc cggtcgattc gcaccaccgc
     9361 actgcggtcg gaggagtatt tgcgcgcgtt ggtgagcgcc tcggcggcta tcttgtaggc
     9421 cgccacctcg caggccggcg acatctccag cagttcgttc tcgtcggcct cgatgcgcag
     9481 atcggccagg gagaggaagg cgcccagatc ggcgagcgcg ccgatgaggc cgagcctcgc
     9541 caactcggcg ggaaacgcgc cgtcgatggc ggcgaggagg cccgagagcg cgtcacccaa
     9601 attgcggatc gcctcgtcca ccgaatccag cgaatcgagc gggtccggac cgccggcgcc
     9661 gtcggcgacg acctcccgcg ccaggtcgaa gcgcagccgc aacgccgcga gggcgggcgc
     9721 catctggtcg tggatctcgc gccgcagccg ctgccgctcc tcctcgatcc ccgccgtcag
     9781 ttcccaggcg accttgccgt tcttctcccg ccggtccatc ggcctccccg ttcggcgccc
     9841 cgtcgtgccg gatcgtacgg caggccgaac cgccggtgga cgaatggtca cggccttcca
     9901 agaaagcgga cgtcgatgcc cacgagttcc cggccgcaaa gccgcagcgc gaccgtggac
     9961 tcgccttcca tctggaattc cgcgtgtccg ccgggcacgt gaacccgcag cgccgcgccg
    10021 gcgacgtcga tttccgcggc ccgcgtctcc acccgcgcca gcaggcccat gacgttttcc
    10081 gggagcccgt ccagcccgat ggtcaggccc ttcccgacgc tcgcccgagc ggggagcccg
    10141 cgcacgtcga cgacgctgca cgcgcgtgcc ggccagggga accagacgcg cagccccgca
    10201 ccgcacaccg ccacgcggac gtcggagtcc accggcgctc cgggcccgcc tttccccttc
    10261 atcgatccca ttttccttgc cgcgtaatgg tccgctcctt cggccgagtc cgtgtcaggg
    10321 cccaccgaac catgcccggt cacccggggg catgggctga aggtctcgac cacccaggag
    10381 gatcggcgcc cggccggcgt caggaaccca cgccgtcccc gccgccgtcg cggcggagcc
    10441 agccgcgctg ggcgcccagg tcgatgagct tgctcgcgta ggcgcccagc tccgcggacg
    10501 cgtcgccgat caccgacttg cggcggcgga cgccggcctc ggtgacgccc ggggtgtgcc
    10561 aggtgccgcc ctcgttgccg aagttgagca gcagcggctg gaggcggtcc atggccttgg
    10621 cgaaccgcgc ctccggggtc gcgcgggcct cgaactcgtc ccacagcgcg cggaacgccc
    10681 cggcctggtc gggcggcagc agcgcgaaca gctcgtcggc ggcggcctcc tcccgctcgg
    10741 cctggtcggc cgacgcggcc tcgtcgtaga ggaaggtgtc gcccgcgtag atctccacga
    10801 ggtcgtgcac caggacgagc cgcagcgtcc gcgcgacgtc gatcggctcg tcggcgtact
    10861 cggcgagcac ggtgaccatc agcgcgaggt gccaggagtg ctcggcgtcg ttctcgcgcc
    10921 ggtcggcggc cagcagcggg ctgcgccgca cgacggtctt cagccggtcc acctcggcga
    10981 tgaacgcgat ctgggacgcg agccgctccg ggacgtcgcg cgggggctcg gccctgccga
    11041 tgaggtcgcg ccgcgtcgtg gaggtcatgg agatcctttc ggtcggggac ccggggccgc
    11101 gcggtctccc gtggttcgcg gctacccggt caacgcatga tcgacaccga gccgttccgg
    11161 gacagacaag cactgttccg acacaggcaa gtacgcgggc tgtgaaggcg gtgaactttg
    11221 cccgaagcgt ccagagcgga cgaggaaggg ggccgccgtg gcagcggacc accagtccgg
    11281 ggtcgacgac aaggtcgaat cgacaccccg gagcgag
//
