LOCUS       NZ_JAHFZY010000001     10903 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Actinomadura sp. NEAU-AAG7 Scaffold1, whole genome shotgun sequence.
ACCESSION   NZ_JAHFZY010000001
VERSION     NZ_JAHFZY010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura sp. NEAU-AAG7
  ORGANISM  Actinomadura sp. NEAU-AAG7
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 2046061)
  AUTHORS   Xu,X. and Zhao,J.
  TITLE     Actinomadura oryzae sp. nov., a novel actinobacterium with the
            activity of releasig potassium isolated from rhizosphere soil of
            rice in Sanya
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2046061)
  AUTHORS   Xu,X. and Zhao,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2021) College of Life Sciences, Northeast
            Agricultural University, 600 Changjiang Road, Xiangfang District,
            Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018603865.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 03:10:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,952
            CDSs (total)                      :: 7,871
            Genes (coding)                    :: 7,813
            CDSs (with protein)               :: 7,813
            Genes (RNA)                       :: 81
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 6 of 58
            Pseudo Genes (incomplete)         :: 53 of 58
            Pseudo Genes (internal stop)      :: 1 of 58
            Pseudo Genes (multiple problems)  :: 2 of 58
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:01:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1657749
            Orig. end                         :: 1668652
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHFZY010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10903
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="10"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10903
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10903
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5903](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5903
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            577..1764
                     /locus_tag="KLI87_RS07540"
                     /old_locus_tag="KLI87_07545"
     CDS             577..1764
                     /GO_function="GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_transf_28"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_tran_28_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017577442.1"
                     /locus_tag="KLI87_RS07540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_07545"
                     /product="glycosyltransferase"
                     /protein_id="WP_214616336.1"
                     /sec_met_domain="Glyco_transf_28 (E-value: 4.7e-15,
                     bitscore: 52.6, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_tran_28_C (E-value: 5.2e-21,
                     bitscore: 72.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHVVVPQALIDRRSPVRLIVSGGGGDHTFPAVAVVRAARTAFRH
                     HGLELQPFWVGAERDVEAKVAAEEGVPFTAIPTGGTRRARNRLCRVSAAVGRAPMAVTE
                     AVNVVRGIRPDAVLCTGGYASVPVGLAARFCHRPLLVHEQGVRVGLANRITMRGASRVA
                     LGTEPTMDLLPERLRGRAVVTGNPLRPGLAGGDAGSAPARLRWDGWAPSLPTVYVTGGA
                     IGARRLNELVTAILPELLRYANVFHQAGAELLGPVLAAAEALPPELRPRYLARGFVGEE
                     LADLLALADVAVSRGGCRTVGELTALGKPSVLVLPARAPGDERRRHAEHLAAHGAARVL
                     AGDEATPARLVRELALLLTDPLRRSEMSAMARRLGRPGAAAALTNEVLALCAAAP"
     gene            2060..3448
                     /gene="murF"
                     /locus_tag="KLI87_RS07545"
                     /old_locus_tag="KLI87_07550"
     CDS             2060..3448
                     /EC_number="6.3.2.10"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="murF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541823.1"
                     /locus_tag="KLI87_RS07545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_07550"
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="WP_214616337.1"
                     /transl_table=11
                     /translation="MIPLTLHEIALAVGGTVHGAPAPGPTVTGPVVIDARDVTPGALFV
                     AVAGERGDGHDLAARAYAFGACAVLGTRAVEGPCVVVDEVVSALTDLAMFVRDMLDLVV
                     IGVTGSVGKTTTKDLLAQILEREAPTVATAGSDGNEIGLPLTILRADRWTRYLVLEMGA
                     ARQGDITHLAWVARPQFGLVLNIGPAHLLTLGGIIGVARAKAELVRALPPAGEGGLAFL
                     NADDAAVASMAGATSAGVVSYGRDAEASVRAGGVTVDGEGRASFILHTPSGAAAVRLGL
                     PGEHQVGNALAAASVADVLGVTTRRIAARLSAAAPRSPNRFQLVERADGITVIDDACHA
                     NPVSMRAALRTLATMSAGRRTIAVLGEMTGQAGDAARHHAAVGRLAAELGVAILVVVGT
                     GEGPDALSEAARRAGVATHALPRRREAVALLRSLLRPADVVLVKASGEIGLSAVATALW
                     NPRS"
     gene            complement(3462..4115)
                     /locus_tag="KLI87_RS07550"
                     /old_locus_tag="KLI87_07555"
     CDS             complement(3462..4115)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLI87_RS07550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLI87_07555"
                     /product="S1 family peptidase"
                     /protein_id="WP_156214180.1"
                     /transl_table=11
                     /translation="MRARIRVIFACVATVMSLLVPGTAAATAPPPSEGGRAIYAANGVR
                     CALGFNVVRSGTYYFLTAGGCAQAGLRIYADPALTVPLGTVAAVTNYKIALVQYVDPKT
                     QRPGTVHTYPGTQEIVTAGAPAAGQRVCRSGPVNHLVCGPVTATNLTINLPDGVINGVA
                     QATVCPGELPGAPYFADTRAVGLEIGRSGSCAGGGSSYFLPVGPILSAFGVAVY"
     misc_feature    complement(4139..4153)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.3"
                     /tool="antismash"
     misc_feature    4208..4224
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     misc_feature    4232..4246
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.0"
                     /tool="antismash"
     misc_feature    complement(4259..4274)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.23"
                     /tool="antismash"
     misc_feature    complement(4270..4283)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.67"
                     /tool="antismash"
     gene            4312..5004
                     /locus_tag="KLI87_RS07555"
                     /old_locus_tag="KLI87_07560"
     CDS             4312..5004
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLI87_RS07555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLI87_07560"
                     /product="hypothetical protein"
                     /protein_id="WP_156214179.1"
                     /transl_table=11
                     /translation="MKTQVDLQFGEFEGGMAKVRREHNGHDRAAGPEVVSSQEYAVEQH
                     YDASGRSFGGASCGGPAEGLEQHVCMGLNACNAFDVDGGAVMAGTGNCATVRHVCHGEG
                     QCRGQGGCGYAGGDDQQWRPGEQACRFNGSCASPLNVSRVFSAGPMKGRSVWLQARRLM
                     EDRMYRAGLAYGPSPGDGIPDDLLPEYDVDDGEDLVGPVGRRGAASGDGARRKTAGKAR
                     KAAPRKAR"
     gene            5001..5903
                     /locus_tag="KLI87_RS07560"
                     /old_locus_tag="KLI87_07565"
     CDS             5001..5903
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619267.1"
                     /locus_tag="KLI87_RS07560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_07565"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_308165886.1"
                     /sec_met_domain="DUF692 (E-value: 4.8e-94, bitscore: 311.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTWADTAADTATGTPAGTAAGMPGFGIGLRGPHMDHVRRHRPDVG
                     FFEVVSENFMDCAGGRRRALEAIAEHYPIALHGVSLSIGGTDPLDLGYLARLRGLAAAV
                     RAPLVSDHVCWTGVMGRNTHDLLPLPFTEAVLAHVVERVRIAQEFLGRRLVLENPSTYV
                     GFTASAMPEQEFMTRLAEEADCGLLLDVNNVYVSAANHDFDPVDYLDALPMERVEQIHL
                     AGHADHGTHLIDTHDRPVSDAVWDLYRLVTERAGPIPTLLEWDDRLPEFPVLEAELGKA
                     RLAAAGRDPVSALQGVPDA"
     gene            5896..6714
                     /locus_tag="KLI87_RS07565"
                     /old_locus_tag="KLI87_07570"
     CDS             5896..6714
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.5"
                     /locus_tag="KLI87_RS07565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_07570"
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_156214178.1"
                     /transl_table=11
                     /translation="MPEPVAGLAGTQRWMQAAILGDGAADGVERTLTASSSLTARERLG
                     IYRRGYRLRLLENMRGLHPGLTRLLGEEMFDRFALDYLDARPSRTYTLTRLDEGFADHL
                     EATRPDGAAHREQWPDLIIDLARLERIITEVVEGPGTEGGPVLRPDDLPAALDLSRGCD
                     LRLETAPCLRLPTFAFPVNEYLAAVRRGEDPEPPAPRPVALAVSRRDYRVTMRELDPAA
                     HRALRALAGGAAVGAVLADPSAEALRRWAGLGYFTRAHRAPEHLEERARR"
     gene            6711..8195
                     /locus_tag="KLI87_RS07570"
                     /old_locus_tag="KLI87_07575"
     CDS             6711..8195
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024306.5"
                     /locus_tag="KLI87_RS07570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_07575"
                     /product="ferritin-like protein"
                     /protein_id="WP_214616338.1"
                     /transl_table=11
                     /translation="MTTTTTRTKARKPASKTKAKTQPKTQPKTQPKTQAKTQAKTQAKT
                     QARTKARAARKAGDRLVRTRPIRRLDPAAGIRTVDDLADHLYVAAQVELSTIPMYLFSA
                     YSIRTRGHSQWAAPRGVLRTLVGISIEEMLHLALVRNLMVAIGYGEDISFYDEEFIPSY
                     PSTMLNRYNPDDPDGPEIILSLDRLSKEHVNTFRRVEMPDDIDLGAETFARHPRDVGQY
                     TSLGEFYRAIEQGFIDLEDEIEWAVEDARKQYKRGFWNEFGNGKPIRVHDLATARQALR
                     IIIEQGEGTITDHKKIEIRPGVEDYTHYEKFVRVQEGIEGIGAVDGGPEHRIDIDDPEA
                     TWPVVADPLVADFDDQPGVQSLMTLFNAAYCYTLCLLDETYAHSTDDVKTKKVPGTDRE
                     EMFSHRYRLERNDVAVMQGILYPIAQALVTTPIPDGPYKGRHAAPSFQFYDFDADEQER
                     TKKEQLIDLCAEAIEHFPVLGGPDGVERQIRLLVDI"
     misc_feature    complement(8264..8282)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.59"
                     /tool="antismash"
     misc_feature    8275..8289
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.12"
                     /tool="antismash"
     gene            8296..9900
                     /locus_tag="KLI87_RS07575"
                     /old_locus_tag="KLI87_07580"
     CDS             8296..9900
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1138: FAD linked oxidase domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013714.5"
                     /locus_tag="KLI87_RS07575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_07580"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_214616339.1"
                     /transl_table=11
                     /translation="MTEHTRRNVLRGGLAATAAGVAAPALGAAPAEAAGPAAPPPPASE
                     VGRGDPRFPSLASRGYNRRFVGDPDHVWVVGTTEDVVTAVQRAVDTGRRVAVRGGGHGF
                     EGFVGDPAVRVVIDTSAMTDVYYDARRRAFAVEAGALLGDVYRRLYLGWGVTIPAGWCP
                     TVGAGGHVPGGGFGPLSRTMGLAVDHLHAVEVVVVGRDGRARSVVATSGASDPNRDLWW
                     AHTGGGGGTFGVATRYWFRTPDARGDDPSRLLPAPPSTALTFSVDWDWSRLDRAAFERL
                     VRNHGTWVERNSAPGSPAAALYSELQLTRPAAGTIGMIGQVAAVAGASAAERLLDGHIA
                     AISEGVPAAPVRKAANQPWLAAALAGSPGDPGPRYRLKVKSAYLRRRLTDRQIGVLHHH
                     LTRTDYDHPGAILSLYTFGGRVNAVRPDATAAPHRDTIMKMFFINGWEDAADDARHIAY
                     TREVYRDLYADTGGVPAAGDGAFINYPDTDLADPALNGGAPWHVLYFRGNYPRLQRIKA
                     KWDPRDVFRHALSVRPV"
     gene            complement(9973..10668)
                     /locus_tag="KLI87_RS07580"
                     /old_locus_tag="KLI87_07585"
     CDS             complement(9973..10668)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLI87_RS07580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLI87_07585"
                     /product="S1 family peptidase"
                     /protein_id="WP_214616340.1"
                     /transl_table=11
                     /translation="MRLRISSAAATAAACAIATLAAPAPGAMASSASPASPAAPPSAVG
                     GQTVYASDGSPCTLGFNVRRSDGYYFLTAGSCARQDQRVYADSAKTTALGTVVARTPLG
                     VGLVRYVEPQAERPGGVDTHPGSQHIKGAGHVPIGQQICRSSPVSGLRCGKLKAVDLSV
                     MLPDGTMGTIAQTDACGLPGDAPGAPYFAGGTALGVEFQGSGACEGGENSYYQQLDEIL
                     VQLGGGEVY"
ORIGIN
        1 gccgaggagg tcgcgctggc ccgccggtgg ggcgcgccgc gtccgctgag cgccgcgctc
       61 gccgcgttcg gcgtggtcgc cgccgacccg gacgcggcgc gcgaggccgt cgccgtcctc
      121 gacgggctgg acgccggact cctccgcgcg gcggcgctca tcgacctcgg cgccgtgctg
      181 ctgcgcgggg gcgacgccga gcaggcccac gagcgcctcc aggacgggtt cgcgctggcg
      241 cacgagatca acgcccggcc gctgtcgctg cgcgcggccc gccacctgcg ccgcgcgggc
      301 ggcaagcccg acctcgggcg gctcagcggc gtcaccgcgc tcaccgcgca ggagcgcgcc
      361 gccgccgacc gcgccgccgc cggcgccacc aaccgccaga tcgccgagga gatggtgctg
      421 acccagcgca ccgtggagca gtacctcacc agcgcctacc gcaagctcgg catcagcggg
      481 cggcgccagc tcgccgccgc cctgtccgcc tgagtccgcc tgagtccgcc tgaaccccgc
      541 cctgtccgcc cgaacgcccc gccccgtgga gtggtcatga cgcatgtcgt cgtcccccaa
      601 gccctgatcg accggcgcag cccggtccgg ctcatcgtca gcgggggcgg cggcgaccac
      661 accttcccgg ccgtcgcggt cgtccgtgcg gcgcggaccg cgttccgcca ccacggcctg
      721 gagctccagc cgttctgggt gggcgccgag cgcgacgtcg aggccaaggt cgcggcggag
      781 gagggcgtgc cgttcaccgc gatccccacc ggcgggacgc ggcgcgcccg caaccggctc
      841 tgccgggtgt cggccgccgt cggccgggcg cccatggcgg tgaccgaggc ggtcaacgtc
      901 gtccggggga tccggcccga cgccgtgctg tgcaccggcg ggtacgcctc ggtgccggtc
      961 gggctggcgg cgcggttctg ccaccggccg ctgctcgtcc acgagcaggg ggtgcgggtg
     1021 gggctcgcca accggatcac gatgcgcggg gcgtcgcgcg tcgcgctcgg caccgagccg
     1081 accatggacc tgctgcccga gcggctgcgg gggcgggcgg tggtgaccgg caacccgctg
     1141 cgccccggcc tcgccggcgg cgacgcgggc tccgcgccgg cgcggctccg ctgggacggc
     1201 tgggcgccct cgctcccgac cgtctacgtc accggcggcg cgatcggggc gcgccggctc
     1261 aacgagctgg tcaccgcgat cctgcccgag ctgctgcgct acgccaacgt gttccaccag
     1321 gcgggcgccg agctgctcgg gccggtgctg gcggcggccg aggcgctgcc gcccgagctg
     1381 cgcccccgct acctcgcgcg cgggttcgtc ggcgaggagc tggccgacct gctcgcgctc
     1441 gccgacgtcg cggtgtcgcg cggcggctgc cggacggtcg gcgagctgac cgcgctcggg
     1501 aagccgtcgg tgctcgtcct gcccgcccgc gcccccgggg acgagcggcg ccgccacgcc
     1561 gagcacctcg ccgcgcacgg cgccgcccgc gtgctggccg gggacgaggc gacgcccgcc
     1621 cggctcgtgc gggagctggc gctgctgctg accgacccgc tgcgccgctc ggagatgtcg
     1681 gcgatggcgc gccgcctcgg ccggcccggc gccgccgccg cgctgaccaa cgaggtcctc
     1741 gccctctgcg cggcggcgcc gtgacctggc gcggggcccg ggactgcccg gtgcgcgggg
     1801 gcgcagaccc cggtcggtcc gggccgaatg cccggtatcc cggtgtgttc ggggcgtttt
     1861 acgatctggc gctaaggggt ggcgccgggg ccggacggga gatcatgcag gcattcatgg
     1921 gcctccggag gggggagtac cgtcccgccg tcggcggggt cgagcgggac ggtacctgga
     1981 ggccggaccc gtccgcggcg gtcgccgagg ccgccgcgga cggggccggt gcgcatcgcg
     2041 ggtcagcgcg gccggtgccg tgatcccgct gaccctccac gagatcgcgc tggcggtcgg
     2101 cggaacggtg cacggcgccc ccgcccccgg tcccaccgtc accgggcccg tcgtcatcga
     2161 cgcgcgggac gtcacccccg gcgcgctgtt cgtcgccgtc gccggggagc gcggcgacgg
     2221 gcacgacctc gccgcgcgcg cctacgcctt cggcgcgtgc gcggtgctcg gcacccgcgc
     2281 cgtcgagggg ccgtgcgtcg tcgtggacga ggtggtctcc gcgctgaccg acctcgccat
     2341 gttcgtccgc gacatgctgg acctcgtcgt catcggcgtc accggctcgg tcggcaagac
     2401 cacgaccaag gacctgctcg cccagatcct cgaacgcgag gcgccgaccg tcgccaccgc
     2461 cggctccgac ggcaacgaga tcgggctgcc gctgacgatc ctgcgcgccg accggtggac
     2521 gcgctacctc gtcctggaga tgggggccgc ccgccagggc gacatcaccc acctcgcgtg
     2581 ggtggcgcgc ccgcagttcg ggctggtgct gaacatcggg cccgcgcacc tgctcacgct
     2641 aggcgggatc atcggcgtcg cgcgggcgaa ggccgagctg gtgcgggcgc tgcccccggc
     2701 gggggagggc gggctggcct tcctcaacgc cgacgacgcg gccgtggcgt cgatggcggg
     2761 cgcgacgtcg gcgggcgtcg tgtcctacgg ccgcgacgcc gaggcgtccg tccgggcggg
     2821 cggcgtcacg gtcgacggcg agggccgcgc gtcgttcatc ctgcacaccc cgtcgggcgc
     2881 ggcggccgtg cggctcgggc tgcccggcga gcaccaggtc ggcaacgcgc tggcggccgc
     2941 gagcgtcgcc gacgtcctcg gcgtcacgac ccgccggatc gcggcgcggc tgagcgccgc
     3001 cgccccgcgc tcgccgaacc ggttccagct cgtcgagcgc gccgacggca tcacggtcat
     3061 cgacgacgcc tgccacgcca accccgtctc catgcgcgcc gcgctgcgca cgctggcgac
     3121 gatgtcggcc ggacgccgga cgatcgccgt cctcggcgag atgaccggcc aggccgggga
     3181 cgcggcgcgg caccacgccg ccgtcggcag gctggccgcc gaactgggcg tcgcgatcct
     3241 cgtcgtggtc gggaccggcg agggccccga cgccctgtcc gaggccgccc gccgggcggg
     3301 cgtggccacc cacgcgctgc cgcgccgccg ggaggcggtg gccctgctgc ggagcctgct
     3361 gcgcccggcc gacgtcgtcc tggtgaaggc gtcgggcgag atcggcctgt ccgcggtcgc
     3421 gaccgcgctg tggaacccgc gctcctagcg ggggaccgcg gtcagtacac cgcgacgccg
     3481 aaggcgctca ggatggggcc gaccggtagg aaataggacg agccgccgcc ggcgcaactg
     3541 ccggacctgc cgatttcgag cccgaccgcc ctggtgtcgg cgaagtaggg cgcgcccggg
     3601 agttcgcccg ggcacacggt cgcctgcgcc acaccgttga tcacaccgtc ggggaggttg
     3661 atggtcaggt tggtcgccgt gacgggcccg cagaccaggt ggttgacggg gccgctgcgg
     3721 cagacccgct ggccggcggc cggggccccg gccgtcacga tctcctgcgt gccggggtag
     3781 gtgtggacgg tgccgggccg ctgcgtcttc gggtccacgt actggacgag ggcgatcttg
     3841 tagttggtga ccgcggcgac cgtgccgagc gggacggtca gcgccgggtc ggcgtagatc
     3901 cgcagaccgg cctgcgcgca gccgcccgcg gtgaggaagt agtaggtgcc gctcctgacc
     3961 acgttgaacc cgagggcgca gcgcacgccg ttggccgcgt agatcgcccg cccgccctcg
     4021 gacggcggcg gggcggtggc cgcggccgtg ccggggacga gcaggctcat gacggtcgcg
     4081 acgcacgcga agatcacacg aatgcgggct ctcacggggg cctcctgccg gggggctgac
     4141 tttccggaat agtgcggaaa agcgcggcca ccggcaaggg gaggaagtgc caatgttggt
     4201 caccgccttg tatcaacatc aggtgatcgc caattttcgg tcaacgtggg cgcatgccgt
     4261 ttccacgtgt cgtaataagt cgagccgcgc gcttactgat cttcgtcccg ggtgaagacg
     4321 caggtggacc tgcaattcgg tgaatttgag ggcggcatgg caaaagtgag gcgcgagcac
     4381 aacgggcacg accgggcggc gggcccggag gtggtgtcct cgcaggagta cgcggtcgag
     4441 cagcactacg acgcgagcgg gcgcagtttc ggcggcgcga gctgcggcgg gcccgccgag
     4501 ggcctggagc agcacgtgtg catgggcctg aacgcgtgca acgccttcga cgtcgacggc
     4561 ggcgccgtca tggccggaac cgggaactgc gcgaccgtcc gccatgtctg ccacggcgag
     4621 gggcagtgcc gcggccaggg cggctgcggc tacgccggag gcgacgacca gcagtggcgc
     4681 cccggggagc aggcgtgccg gttcaacggg agctgcgcga gcccgctcaa cgtcagccgc
     4741 gtgttcagcg ccgggccgat gaagggcagg agcgtgtggt tgcaggcccg gcggctcatg
     4801 gaggaccgga tgtaccgggc gggcctcgcc tacggcccgt ccccgggcga cgggatcccc
     4861 gacgacctgc tgcccgagta cgacgtcgac gacggcgagg acctggtggg cccggtgggg
     4921 cggcgcggcg ccgcgtccgg ggacggcgcc cgccgcaaga ccgccgggaa ggcccggaag
     4981 gccgcgccga ggaaggcgcg gtgacctggg cggacaccgc cgcggacacc gcaacgggca
     5041 ccccagcggg caccgccgcg ggcatgccgg ggttcgggat cgggctgcgc gggccgcaca
     5101 tggaccacgt ccggcggcac cgcccggacg tcggcttctt cgaggtggtc tcggagaact
     5161 tcatggactg cgccggcggc cgccgccgcg cgctggaggc gatcgccgag cactacccga
     5221 tcgcgctgca cggggtctcg ctgtcgatcg gcggcaccga cccgctcgac ctcggctacc
     5281 tggcgcggct gcgcgggctg gccgcggcgg tgcgggcgcc gctggtgtcc gaccacgtct
     5341 gctggacggg ggtcatgggc cgcaacaccc acgacctgct tccgctcccc ttcaccgagg
     5401 cggtgctggc gcacgtggtg gaacgcgtcc ggatcgcgca ggagttcctc ggccgcaggc
     5461 tggtgctgga gaacccgagc acctatgtcg ggttcaccgc gtccgcgatg cccgagcagg
     5521 agttcatgac ccggctggcc gaggaggccg actgcgggct gctgctggac gtcaacaacg
     5581 tctacgtctc ggcggccaac cacgacttcg acccggtcga ctatctcgac gcgctgccga
     5641 tggagcgcgt cgagcagatc catctggccg ggcacgccga tcacgggacg catctcatcg
     5701 acacccacga ccgcccggtc tccgacgccg tctgggacct ctaccggctc gtgaccgagc
     5761 gcgccgggcc gattcccacg ctgctggaat gggacgaccg gctgccggag ttccccgtcc
     5821 ttgaggccga actcggcaag gccaggctcg ccgccgccgg acgcgacccg gtctcggcgc
     5881 tccagggcgt accggatgcc tgaaccggtg gcggggctgg ccgggaccca gcggtggatg
     5941 caggccgcca ttctcggcga tggcgcggcc gacggcgtcg aaaggactct cacggcgtcg
     6001 tcgtcgctga ccgcgcggga gcggctcggg atctaccggc gcggctaccg gctccggctg
     6061 ctggagaaca tgcgcggcct gcaccccggg ctgacgcggc tgctgggcga ggagatgttc
     6121 gaccggttcg ccctggacta cctcgacgcg cgcccgtccc ggacgtacac gctcacccgg
     6181 ctcgacgagg ggttcgccga ccacctggag gcgaccaggc cggacggcgc ggcgcaccgc
     6241 gagcagtggc cggacctgat catcgacctg gcccggctgg agcggatcat cacggaggtc
     6301 gtcgagggcc ccggcaccga gggcgggccc gtcctgcgcc cggacgacct cccggcggcc
     6361 ctcgacctct cgcggggctg cgacctgcgt ctggagacgg cgccgtgcct gcggttgccg
     6421 acgttcgcct tccccgtcaa cgagtacctg gcggccgtcc ggcgcggcga ggacccggag
     6481 ccgcccgcgc cgcgtccggt cgcgctggcg gtgagccgcc gcgactaccg ggtcacgatg
     6541 cgcgagctcg accccgccgc gcaccgggcg ctgcgggcgc tggccggggg cgcggcggtc
     6601 ggcgcggtcc tcgcggaccc gtccgccgag gcgctccgcc gctgggccgg actcggctac
     6661 ttcacccgcg cccaccgcgc acccgaacac cttgaggaga gggcgagacg atgaccacca
     6721 ccacgacccg gacgaaggcg cgcaagcccg cgtccaagac gaaggccaag acacagccca
     6781 agacgcagcc caagacgcag cccaagacgc aggccaagac gcaggccaag acgcaggcca
     6841 agacgcaggc caggacgaag gcgcgcgccg cgcggaaagc gggcgaccgg ctggtgcgga
     6901 cgagaccgat ccgccggctc gacccggcgg cgggcatccg gaccgtggac gacctggccg
     6961 accacctgta cgtcgccgcg caggtggagc tgtccacgat cccgatgtac ctgttctccg
     7021 cgtactcgat ccggacgcgc gggcactcgc agtgggcggc gccgcggggc gtgctgcgca
     7081 cgctggtcgg gatctcgatc gaggagatgc tgcacctggc gctcgtgcgc aacctgatgg
     7141 tcgccatcgg gtacggcgag gacatctcct tctacgacga ggagttcatc ccgtcgtatc
     7201 cgagcacgat gctgaaccgc tacaaccccg acgacccgga cggcccggag atcatcctca
     7261 gcctggaccg gctctccaag gagcacgtca acaccttccg ccgggtcgag atgcccgacg
     7321 acatcgacct cggcgcggag acgttcgcgc ggcacccgcg ggacgtcggc cagtacacga
     7381 gcctgggcga gttctaccgc gcgatcgagc aggggttcat cgacctggag gacgagatcg
     7441 agtgggcggt ggaggacgcg cgcaagcagt acaagcgcgg gttctggaac gagttcggca
     7501 acggcaagcc gatccgcgtc cacgacctcg ccaccgcccg gcaggcgctg cggatcatca
     7561 tcgagcaggg cgagggcacg atcaccgacc acaagaagat cgagatcagg cccggcgtcg
     7621 aggactacac ccactacgag aagttcgtcc gcgtccagga gggcatcgag ggcatcggcg
     7681 cggtcgacgg cggccccgag caccggatcg acatcgacga cccggaggcg acctggcccg
     7741 tcgtcgccga cccgctggtg gccgacttcg acgaccagcc cggcgtccag agcctgatga
     7801 cgctgttcaa cgcggcctac tgctacacgc tgtgcctgct ggacgagacc tacgcgcatt
     7861 ccaccgacga cgtgaagacg aagaaggtcc cgggcaccga ccgcgaggag atgttcagcc
     7921 accggtaccg gctcgaacgc aacgacgtcg cggtgatgca agggatcctc taccccatcg
     7981 cgcaggcgct ggtcacgacg ccgattccgg acggccccta caaggggcgg cacgcggcgc
     8041 cctccttcca gttctacgac ttcgacgcgg acgagcagga gcgcaccaag aaggagcagc
     8101 tcatcgacct gtgcgcggag gccatcgagc atttccccgt tctcggcggc ccggacggcg
     8161 tcgaacggca gatccggctt ctcgtcgaca tctgacatcc gggggttgcg cctaccgatg
     8221 aggtggggcc aggcccactg accgcatccc gcgccgtccc tacgttggtg gtcattcgcg
     8281 atctgaggag tccggatgac cgagcacacg cggaggaacg tcctgcgggg cgggctggcg
     8341 gccacggcgg cgggggtcgc cgcgcccgcc ctgggcgcgg caccggccga ggcggccggg
     8401 cctgccgcgc cgccgcctcc ggcgtcggag gtcggacgcg gcgacccgcg cttcccgtcc
     8461 ctggcctcgc gcggctacaa ccggcggttc gtgggcgatc ccgaccacgt gtgggtggtc
     8521 gggacgaccg aggacgtggt cacggcggtg cagcgggccg tcgacaccgg caggagggtg
     8581 gcggtccgcg gcggcggcca cggcttcgag ggcttcgtcg gcgatcccgc cgtgcgcgtc
     8641 gtcatcgaca cctccgcgat gaccgacgtc tactacgacg cccgccgccg cgctttcgcg
     8701 gtggaggcgg gcgcgctgct cggcgacgtg taccgcaggc tctacctggg atggggcgtg
     8761 acgatccccg ccgggtggtg cccgaccgtc ggcgccggcg ggcacgtgcc cggcggcggc
     8821 ttcggcccgc tgtcgcggac gatgggcctc gccgtcgacc acctccacgc ggtcgaggtg
     8881 gtcgtcgtcg gccgggacgg gcgggcccgg agcgtggtgg cgacgagcgg ggcgtccgac
     8941 ccgaaccggg acctgtggtg ggcgcacacc ggcggcgggg gcgggacgtt cggcgtcgcc
     9001 acccgctact ggttccggac gccggacgcg cgcggcgacg acccgtcccg gctgctgccc
     9061 gcgccgccgt ccacggcgct gacgttctcc gtcgactggg actggagcag gctggacagg
     9121 gccgcgttcg agcggctcgt ccgcaaccac ggcacctggg tggagcgcaa cagcgcgccg
     9181 ggctccccgg cggccgcgct ctacagcgaa ctccagctca cccgcccggc ggccgggacg
     9241 atcggcatga tcggccaggt cgccgccgtc gcgggcgcgt ccgccgccga gcggctgctg
     9301 gacggccaca tcgccgcgat cagcgagggt gtgccggccg cacccgtcag gaaggcggcg
     9361 aaccagccgt ggctcgcggc cgcgctagcc gggtcgccgg gcgacccggg gccgcggtac
     9421 cggctgaagg tgaagtcggc ctacctgcgg cgccgcctga ccgaccggca gatcggcgtc
     9481 ctgcaccacc acctgacccg caccgactac gaccaccccg gcgcgatcct cagcctctac
     9541 accttcggcg ggcgggtcaa cgccgtccgc ccggacgcga cggcggcgcc ccaccgcgac
     9601 acgatcatga agatgttctt catcaacggg tgggaggacg ccgccgacga cgcccggcac
     9661 atcgcctaca cgcgcgaggt ctaccgcgac ctctacgccg acacgggcgg cgtgcccgcg
     9721 gccggcgacg gcgcgttcat caactacccc gacaccgacc tcgcggaccc ggcgctgaac
     9781 ggcggcgcgc cgtggcacgt cctgtacttc cgcggcaact acccgcggct ccagcggatc
     9841 aaggcgaagt gggatccgcg cgacgtcttc cgccacgcgc tgtccgtccg ccccgtgtga
     9901 tcgcgctcgg cgcggcgcgg ctcggcgcgg cgcggcgcgg ctccggggcc ggtggtcggg
     9961 atcgggacgt gctcagtaga cctcgccgcc tcccaactgc acaaggatct cgtcgagctg
    10021 ctggtagtag gagttctcgc cgccctcgca ggcgccggag ccctggaact ccacgccgag
    10081 cgcggtcccg ccggcgaagt agggcgcgcc gggggcgtcg ccgggcagcc cgcaggcgtc
    10141 ggtctgcgcg atggtgccca tcgtgccgtc gggcagcatc acgctcaggt cgaccgcctt
    10201 cagcttgccg cagcgcagcc cggagaccgg gctgctgcgg cagatctgct ggccgatggg
    10261 cacgtggccc gcgcccttga tgtgctgcga accggggtgg gtgtcgacgc cgccgggacg
    10321 ctccgcctgc ggttcgacat agcggacgag cccgacgccg aggggcgtgc gcgcgacgac
    10381 ggtgccgagc gcggtggtct tggcggagtc ggcgtagacc ctctggtcct gccgcgcgca
    10441 cgaaccggcg gtgaggaagt agtagccgtc gctcctgcgg acgttgaaac cgagcgtgca
    10501 cggggagccg tcgctcgcgt agacggtctg gccgccgacg gcggagggcg gggcggcggg
    10561 ggaggcgggg gaggcggatg atgccatcgc gcccggggcg ggagcggcga gcgtggcgat
    10621 ggcgcaggcg gcggcggtcg cggccgccga ggagatgcgg agtctcacgg gggtctcctg
    10681 cggggatgga cgcccagggt cctgggttgc cggaatagtc cggccgggcg cgggccccgg
    10741 caaggggggg aacctgccaa gacggccgcc gaccgtatat caactacggg gcaggcggcc
    10801 ggaaggcgcc ggtcagcgga gctcagcccg cgccgggcgc gtttgcggac gtccgttccg
    10861 agctgcgcgt ccatgcctcg gcgatcagtt cgaaggagcg gac
//
