LOCUS       NZ_JAHFZY010000001     11905 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Actinomadura sp. NEAU-AAG7 Scaffold1, whole genome shotgun sequence.
ACCESSION   NZ_JAHFZY010000001
VERSION     NZ_JAHFZY010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinomadura sp. NEAU-AAG7
  ORGANISM  Actinomadura sp. NEAU-AAG7
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 2046061)
  AUTHORS   Xu,X. and Zhao,J.
  TITLE     Actinomadura oryzae sp. nov., a novel actinobacterium with the
            activity of releasig potassium isolated from rhizosphere soil of
            rice in Sanya
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2046061)
  AUTHORS   Xu,X. and Zhao,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2021) College of Life Sciences, Northeast
            Agricultural University, 600 Changjiang Road, Xiangfang District,
            Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018603865.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 03:10:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,952
            CDSs (total)                      :: 7,871
            Genes (coding)                    :: 7,813
            CDSs (with protein)               :: 7,813
            Genes (RNA)                       :: 81
            rRNAs                             :: 5, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 6 of 58
            Pseudo Genes (incomplete)         :: 53 of 58
            Pseudo Genes (internal stop)      :: 1 of 58
            Pseudo Genes (multiple problems)  :: 2 of 58
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:01:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 113517
            Orig. end                         :: 125422
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAHFZY010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11905
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11905
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11905
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6905](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6905
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(16..30)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.18"
                     /tool="antismash"
     misc_feature    complement(832..847)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.19"
                     /tool="antismash"
     misc_feature    complement(837..854)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.07"
                     /tool="antismash"
     gene            950..2326
                     /gene="gdhA"
                     /locus_tag="KLI87_RS00510"
                     /old_locus_tag="KLI87_00510"
     CDS             950..2326
                     /EC_number="1.4.1.4"
                     /GO_function="GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /GO_process="GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="gdhA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593591.1"
                     /locus_tag="KLI87_RS00510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_00510"
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="WP_214615417.1"
                     /transl_table=11
                     /translation="MPLSPRLQAPPETLALLEESYERVLRRDPGEPEFHQAVHEVLESL
                     PPVLAARPGLAAAKMVERVIEPERQIVFRVPWQDDAGEIHVNRGFRVEFNSALGPYKGG
                     LRFHPTVNLGVVKFLGFEQIFKNALTGLGIGGGKGGSDFDPHGRSDAEVMRFCQSFMTE
                     LQRHIGDHTDVPAGDIGVGGREIGYLFGQYRRVTNRWEAGVLTGKGLPWGGSAGRTEAT
                     GYGNVMFTAEMLRRRGEDLDGQQVVVSGSGNVAIYTIEKAQALGANVVTISDSGGYVVD
                     EKGIDLDLLKKVKEVDRGRVSDYAEQRGDSARYVAGGSVWDVPADIALPSATQNELDES
                     AAAVLVRNGVKAVSEGANMPATPGAVHVFQGAGVAFGPGKAANAGGVAVSALEMQQNAG
                     RESWTFERVESELSGIMTSIHDTCFETAERYGAPGDYVVGANIAGFERVADAMLAQGLI
                     "
     gene            complement(2842..2979)
                     /locus_tag="KLI87_RS00515"
                     /old_locus_tag="KLI87_00515"
     CDS             complement(2842..2979)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLI87_RS00515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLI87_00515"
                     /product="hypothetical protein"
                     /protein_id="WP_214615418.1"
                     /transl_table=11
                     /translation="MVLAQRHPLEQRDGRRQRLLAEHRRRRSDGALRAARSASVGAAAG
                     "
     gene            complement(3459..4703)
                     /locus_tag="KLI87_RS00520"
                     /old_locus_tag="KLI87_00520"
     CDS             complement(3459..4703)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KLI87_RS00520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KLI87_00520"
                     /product="hypothetical protein"
                     /protein_id="WP_156217439.1"
                     /transl_table=11
                     /translation="MTGPNLGTGSANSYRALFAIPGTARLYFGYLLARMPVGVIGLSLL
                     LLGQDTGGTARSGLLVGAFSAALMIATPLLGRALDTFPPGRVLSLAATAQCAALALLAI
                     CWSTSEPETGLLLTGALLAGASLPPAGPALRVALPVFVPDDLLTTGFTAEAVILETVYI
                     IGPLLVGGLGAVAGWLPLAAAGGCTAAGVAVMLSSGRLAGACVDTPPARALWTLGPLAD
                     GGVRRLLLGAFLFLPALAVVEVGVAAVAGERDRPELLGVVMAFSAVASLAGGLLFGLRR
                     WPGSLASHVAVLAGWFAAASVLGALAPGPWPAGAAVVLGSLVVAPALALLGRIGGVVAP
                     EGRAAETYTWLSTMNNCGQAATLPLAGLLAERSGPEGAFLLAATLAGTAALFILNNRRH
                     LAPTTTSRPRETVRT"
     gene            5001..6905
                     /locus_tag="KLI87_RS00525"
                     /old_locus_tag="KLI87_00525"
     CDS             5001..6905
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /locus_tag="KLI87_RS00525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_00525"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_214615419.1"
                     /sec_met_domain="TIGR03975 (E-value: 4.1e-157, bitscore:
                     520.9, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 4.7e-11, bitscore: 40.1,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVTLAVMPWHLMTAPNGAAGVLAAALAPDHEVTTYYGSISFAEH
                     LLGEAETAEEGTARARLLPEAYMYVAEEGFTGGIGEWLFTGALYGHRSFPDEASAACAA
                     IGFDPDVAEQIFELAPAFADAAAQEIIAGRPEVVGFTTTFSQTIPALAVARRLRALDPD
                     ITIVFGGSNCDDTMGAALHRCFPEIDFVVRREGEAPLRSLLAALAEGGQGRPASIPGLC
                     WRDADGTSRANPEGGALPSGAQFPPMDQSAYFERLEESPVADYIQPALMMESARGCWWG
                     ERHHCTFCGLSDLILPFRSKAPDRVVEELLAGIERHQVLDVLTTDNIVDKNYYETVFPT
                     LEKSDLDLRVMYEIKSNITPEQARALRGAGVIQVQPGIENLHSLPLKLMRKGVTGANNV
                     ATLRELQEQSLTVLWNYLVGFPGETDGCYQEVITQIPLLYHLQPPLGAHRIALERFNPY
                     FDHPDLGFAERRPRSWYRGVFPDLGETDLNDVAYLFETPDRGVGGATLAGLKAAFDAWR
                     EAYATSALTHRAVAGRLHLYDRRAGRRAADHVLDEPTEVAAYAALGRGRSPEGLARHLS
                     AGGWPVDAAWASAFIDDLTALGLVFRESGRAVTLSTGHDATAVRGTAIPTPAMVGAGT"
     gene            6922..7629
                     /locus_tag="KLI87_RS00530"
                     /old_locus_tag="KLI87_00530"
     CDS             6922..7629
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039872.4"
                     /locus_tag="KLI87_RS00530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_00530"
                     /product="DUF5825 family protein"
                     /protein_id="WP_214615420.1"
                     /transl_table=11
                     /translation="MIRDLPPVDLTSAAGVAAARGRPDVRVLEVSLADLASGTAVPRGK
                     QEPLTWLRVRPTGAELDGGPWPGDDALGALPAAGVVGLTLEAPALPRAPWLIAFLVRAT
                     SFQLPLEWGGPVAGLPCGLLFHLAPPAFGDEVAGKWRAAHRYGQCYWRRGPGFAAVQDL
                     REDPGAHFVIHEPGLLALFHRLADPVEVADLGADDRAHLRDLLDARLAVELGGVAVGLP
                     YRLRRWPAPVIDF"
     gene            complement(7616..8185)
                     /locus_tag="KLI87_RS00535"
                     /old_locus_tag="KLI87_00535"
     CDS             complement(7616..8185)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887080.1"
                     /locus_tag="KLI87_RS00535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_00535"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_156217436.1"
                     /transl_table=11
                     /translation="MSIQARRERERAERERAIVTAARELAEAEGWDAVTTRRLAAEIEY
                     SQPILYSHFSGKDAIMAAVALEGLADLAAVLRAARPGGIAAVAAAYAEFGERRPALYDA
                     MLTLAVDLEFASPESPAPLREGFGELRETMRPYAGDDDLEMLTETFWSGLHGLHMLMRH
                     GRLRRADHERRLALLIGRFSSPVRSR"
     misc_feature    complement(7979..7981)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(8185..8198)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.96"
                     /tool="antismash"
     misc_feature    complement(8210..8230)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.68"
                     /tool="antismash"
     gene            8275..8646
                     /locus_tag="KLI87_RS00540"
                     /old_locus_tag="KLI87_00540"
     CDS             8275..8646
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887062.1"
                     /locus_tag="KLI87_RS00540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_00540"
                     /product="DUF4267 domain-containing protein"
                     /protein_id="WP_156217435.1"
                     /transl_table=11
                     /translation="MLAPIAYGLAVLLSLFVLFIGARFLVQPLPAAAGYGVPAKPDGDP
                     AYLSVKGVRDAAFGLLGLALVAFSGADAAAWYMLVVALVPLGDTVIVLRNGGTRAAAFG
                     IHFATALVVLLSAALLFAV"
     gene            8733..10205
                     /locus_tag="KLI87_RS00545"
                     /old_locus_tag="KLI87_00545"
     CDS             8733..10205
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012298.5"
                     /locus_tag="KLI87_RS00545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_00545"
                     /product="protein kinase"
                     /protein_id="WP_308165792.1"
                     /sec_met_domain="Pkinase (E-value: 4.9e-38, bitscore:
                     127.8, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDDLRPGDPRAVGRYTLTARLGAGGMGQVYLGSSPGGRPVAVKLV
                     HAGFAADAEFRRRFKREVEAARRVGGFHTAPVVDADPDADTPWLVTAYVPGPSLASAVA
                     EHGPLPPASARVLGAGLAEALEAVHGAGVVHRDLKPSNVLIAADGPRVIDFGIARAVDA
                     SGITTRAGTPGFMAPELITDGASTPACDVFALGAVLAFAVGVRPFGEGPSEALTYRVVH
                     REPDLGGVPEPLGPLVAACLAKDPAARPAPSDLLGALTPGGDAGAWLPPPVQEMVTRFP
                     TAHTPTPRPAPTRQLPSPQAAPPARQAVRIAGRITPAAIALAASVLYSLIWVAIFLLGA
                     GLALGGTSSDADSVATLIGVVVAIVGAVNCVRAIPRAVWQAMALARPVTFEIGPGGLSL
                     RHGGRRVHYGWHEIGRVVLRRIDDKKKRALCVSLNPGSAQPDYLSPRSPFCYTEADTGW
                     VVIAPLIRLRTSRREIEGIVVRYAGPRWGGHA"
     gene            complement(10210..10914)
                     /locus_tag="KLI87_RS00550"
                     /old_locus_tag="KLI87_00550"
     CDS             complement(10210..10914)
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012422.5"
                     /locus_tag="KLI87_RS00550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KLI87_00550"
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_308165793.1"
                     /transl_table=11
                     /translation="MTSILLCSANADCRDRVAGVLGRTLGGQVRLVVPDGRSVAAALVA
                     AATDLLVVDLSPWRAGHGDLVRAVCREGRVLRVPVVLLVDHTARFGEAAGRPELLAALW
                     AGVASIADRRGPVAEVTAAARTAAAGGRYLAPAFACCLLDDPVDGPALLDELFEADPHR
                     PGVLTPREREVLALLASRPSNAWIAERLALSEKTVKYHVSNVLGKLGLRDRGEAAALAA
                     SMSPDHCGTLAS"
     misc_feature    complement(11008..11023)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.88"
                     /tool="antismash"
ORIGIN
        1 agaggacggt tccggtgaac atgatcgcta ggagggtcag cccctcccag gcgtagacgc
       61 ggctgttggc ggccgccagc gcggcggcca gcaggccgcc gaggacgccg ccgaagacgc
      121 gcaggccggg gcggtccgac acggcggcgg cgatcgagat gaccagaccg atggtgacgg
      181 cggcgacgag ggggccgatg cccgcggtgc gcgagagcag ggtggagggg atgaggccgc
      241 ccacgagcac cgcggccagt gcgaatccgg aggcgaccgg cgcggagcgg cggtgcccgc
      301 cgacgacgaa caacccgacg ataaggaggt agaaggccat ctcgtaggtc agcgtccaca
      361 ggacgttgat ggcattgggg acggcgagaa gatcctgcaa catggtcaga tgcgccagga
      421 cggctgtcac cgggtcgtac tgttccagcc ccgcgcgcag cccgaggatg ccgaggatga
      481 aggggagcac cgccgccagg cagcacagcg cgagcatggg gtagatgcgg aagaagcgtc
      541 cgatccaaaa gccccggacg ctgccgcggc gctccagaga ggccggtacg atatagccgc
      601 tgaccaggaa gaacacgagg acgccgtagc ggccggggtc gaagcgttcg gccatccacc
      661 tctgcacgtc cgggatgtag acatagctcg cgtgatggag cgcgaccgtg atcgccgccc
      721 atccccgcag cgcgtccaac cagccgagtc gggcgattgc ggaggactca accgcattgg
      781 ggatcatccc gacagagtag ggcgcatggc gccctggggt ggtccggttg tcagatcatc
      841 cggtcaaggg gaagcgggcc ggcgttcgca ccgcgcggat gatcgcgggg tgcgaggatg
      901 gtgctcccgg gcaggtacaa aggttgttgt tctgcgaaag gaatcaccta tgcccttgtc
      961 cccccgcctc caggcaccgc cggagacact ggccctgctg gaggagtcct acgagcgggt
     1021 cctgcgccgc gacccgggcg agcctgagtt ccaccaggcc gtccacgagg tgctggagtc
     1081 actcccgccc gtgctggccg cgcgacccgg gctcgcggcc gcgaagatgg tggagcgggt
     1141 catcgagccg gagcggcaga tcgtcttccg cgtcccatgg caggacgacg cgggcgagat
     1201 ccacgtcaac cgcgggttcc gggtcgagtt caacagcgcg ctcgggccgt acaagggcgg
     1261 gctgcggttc cacccgacgg tgaacctcgg cgtggtgaag ttcctcggct tcgagcagat
     1321 cttcaaaaac gcgctgaccg ggctcggcat cggcggcggc aagggcggca gcgacttcga
     1381 cccgcacggc cgctccgacg ccgaggtcat gcggttctgc cagtcgttca tgaccgagct
     1441 gcagcggcac atcggcgacc acaccgacgt cccggcgggc gacatcgggg tcggcggacg
     1501 cgagatcggc tacctgttcg ggcagtaccg gcgggtcacc aaccgctggg aggcgggcgt
     1561 gctcaccggc aagggcctgc cgtggggcgg atccgcgggc cggacggagg ccaccggcta
     1621 cggcaacgtc atgttcaccg ccgagatgct gcggcggcgc ggggaggacc tcgacggcca
     1681 gcaggtcgtc gtgtccggct cgggcaacgt ggcgatctac accatcgaga aggcgcaggc
     1741 gctcggggcg aacgtcgtca cgatctccga ctccggcggc tacgtggtgg acgagaaggg
     1801 catcgacctc gacctgctca agaaggtcaa ggaggtcgac cggggccgcg tctccgacta
     1861 tgccgagcag cgcggcgact cggcgcggta tgtggcgggc ggcagcgtct gggacgtgcc
     1921 cgccgacatc gcgctcccgt ccgcgaccca gaacgagctg gacgagagcg ccgcggcggt
     1981 gctcgtccgc aacggcgtca aggccgtctc ggagggcgcg aacatgccgg cgacgcccgg
     2041 cgcggtgcac gtgttccagg gcgcgggcgt cgcgttcggg ccgggcaagg ccgccaacgc
     2101 gggcggcgtc gcggtgagcg cgctggagat gcagcagaac gcgggccgcg agtcgtggac
     2161 gttcgagcgg gtcgagtccg agctgtcggg gatcatgacg tccatccacg acacctgctt
     2221 cgagaccgcc gagcggtacg gcgcgcccgg cgactacgtc gtcggcgcca acatcgcggg
     2281 cttcgagcgc gtcgccgacg cgatgctcgc gcagggcctg atctagaccg cagtcgatcg
     2341 cgggcccgcc caacggcgac ctcgcccagg gcgggcccgt ccggcagccg gtccgcttcg
     2401 cagcagggct cggcacagcg cgcccggctg ggcggggccg tccgctgtgc accctttcct
     2461 gcggctcgcc gccgcaggcc agcacctcgg cgtacgcaac tcggctggga tgcccgtgtc
     2521 cggcaaccgg tccgccctac agcgggcttg cgcagcgagc ccagcctggg aaagggtcgt
     2581 ccaacggcgg ccctgttgga gcgatcgcgc cgcagccact caggcgcaac acctcggcgt
     2641 aggcgattcg gcttggggcg tgctcggcgt aggcgattcg gctgaggcgg gctcggcttg
     2701 gggcggggct gctgacgacg gggccgcttg cagcgagctc ggcatggtga gtccagtgca
     2761 gtgggcgtgg gtaagggcgc gcccatcagg cgcttggcct gacttgcagc ggggctgact
     2821 cagcaggtct ggcttgagga gctacccggc ggcagccccg acgctggcac tgcgggcggc
     2881 ccggagcgcg ccgtcgctac ggcggcggcg gtgctcggcg aggaggcgct ggcggcggcc
     2941 gtcccgctgc tccagcgggt ggcgctgcgc caggaccatc aagcgggctc ggtgtaacgg
     3001 cttcggtgca gcgcgctcgg cataggtgcc tcggtcttgg aatgggctcg gcttgggcgg
     3061 cgcgtccgac ggcaggggcg cttgcggcgg gttgggcgta gcgggttcgg cgcaacgagc
     3121 gcgaagaggg atgggcccgt ctggcggatg gcctgcatcg cagcgggccc ggctcagcag
     3181 agtcggcttg ggtcctcaga cggcggtcct cttgcagcgg gcgcggcgtg gcggcttcga
     3241 tggcctgcgc tcggcttggt ggggcgggct cggcttggga ggtccgtccg ggggcagggc
     3301 cgcctgcggc ttgggttggg tggttggcgg cgggggcgcg ttgtggcggc ttggtgttgg
     3361 gggcagggca aggggtgcgg cgggtcaggt tgggttcggt ggcggtaggg atggcttgcg
     3421 gtgaggatgg cttaggcagt tcggtgtggg ggctggggtt aggttcttac tgtttctctg
     3481 gggcgggacg tggtggtggg tgcgaggtgg cggcggttgt tcaggatgaa cagggctgcg
     3541 gtgccggcca gggtggctgc caggaggaag gcgccttcgg ggccgctgcg ttcggccagg
     3601 aggccggcca gggggagggt cgccgcctgg ccgcagttgt tcatggtgga cagccaggtg
     3661 taggtctcgg cggcgcggcc ctcgggggcg acgacgccgc cgatccggcc gaggagggcg
     3721 agggcggggg cgacgacgag gctgcccagg acgaccgccg cgccggccgg ccaggggccc
     3781 ggggccaggg cgccgaggac ggacgccgcc gcgaaccagc ccgcgagcac cgccacatgg
     3841 gaggccagcg accccggcca gcggcgcagg ccgaacagga ggccgcccgc caggctcgcg
     3901 acggcggaga acgccatcac gacgccgagc agttcggggc ggtcgcgctc tcccgcgacg
     3961 gcggcgacgc ccacttcgac gaccgcgagc gcgggcagga aaaggaacgc gccgagcagc
     4021 agccggcgga cgccgccgtc ggcgagcggg ccgagcgtcc acagggcgcg ggcgggcggg
     4081 gtgtcgacgc aggcgccggc gaggcgtccg ctggacagca tcaccgcgac cccggcggcg
     4141 gtgcagccgc ccgccgccgc gagcggcagc cagcccgcga ccgcgccgag cccgccgacc
     4201 agcagcgggc cgatgatgta gacggtctcc aggatgacgg cctcggcggt gaaaccggtg
     4261 gtcagcaggt cgtcggggac gaagacgggc agcgcgaccc gcagtgcggg cccggccggg
     4321 ggcagggacg cgcccgcgag gagcgcgccc gtgaggagca ggccggtctc cggctcggag
     4381 gtcgaccagc agatcgccag gagggcgagc gccgcgcact gggcggtcgc ggccagggag
     4441 agcacccgtc cgggagggaa ggtgtcgagg gcgcggccga gcaggggggt cgcgatcatg
     4501 agcgcggcgg agaacgcgcc gaccagcagg ccggagcgcg cggtgccgcc ggtgtcctgg
     4561 ccgagcagga ggagggagag cccgatgacg ccgaccggca tccgggcgag cagatatccg
     4621 aaatacaggc gcgcggtgcc cggaatggcg aacagggcac gataggagtt ggcgcttccc
     4681 gtccccaggt tcggccccgt cattttcacc tccagcaccc aggtaagcac ggtccgcaca
     4741 cggcttggcg gaaaagtgcc tgatcttggc cgacatcggc caccccggtg cggtggccct
     4801 cgtcgcggcg ggtagaaaag gtgcgtgaaa cttccagccc agaagggagg acccgtgaag
     4861 cgaaaggtaa tccgtaagca gcagacggcc agccagcgcc acgagttctg acggctcccg
     4921 agcgtggccg ggcccctttt cgggttcgcg ccacgtttcc gtccggcact cgtttcccat
     4981 ccaccaccgg aggtcgacac gtgcgggtga ccctcgcggt catgccctgg cacctcatga
     5041 ccgcgccgaa cggcgcggcc ggcgtcctgg ccgcggccct cgcgccggac cacgaggtca
     5101 ccacgtacta cggcagcatt tccttcgccg agcacctgct cggcgaggcg gaaacggccg
     5161 aggaaggcac cgcgcgagcg cgattgctgc ccgaggcgta catgtacgtc gccgaagagg
     5221 gattcaccgg cggaataggt gaatggcttt tcaccggcgc gctctacggg caccggagtt
     5281 tcccggacga ggcgtccgcc gcctgcgccg cgatcggctt cgacccggac gtcgcggagc
     5341 agatcttcga gctcgccccg gcgttcgccg acgccgcggc gcaagagatc atcgcgggcc
     5401 gtcccgaggt cgtggggttc accacgacgt tctcgcagac cattccggcg ctcgcggtcg
     5461 cacggcggct gcgggcgctc gatccggaca tcacgatcgt cttcggcggc tccaactgcg
     5521 acgacaccat gggcgccgcg ctgcaccggt gcttcccgga gatcgacttt gtggtgcggc
     5581 gggagggcga ggcgccgctg cgctcgctgc tggccgcgct ggccgagggc ggccaagggc
     5641 ggccggcctc gattcccggc ctgtgctggc gcgacgcgga cgggacgtcc cgcgcgaacc
     5701 ccgagggcgg cgcgctgccg agcggcgcgc agttcccgcc gatggaccag agcgcctatt
     5761 tcgagcggct ggaggaatcg ccggtcgccg actacatcca gcccgcgctg atgatggagt
     5821 cggcgcgcgg atgctggtgg ggcgagcggc accactgcac tttctgcggg ctctccgatc
     5881 tcattctgcc gttccggtcg aaggcgccgg accgggtcgt cgaggagttg ctcgcgggca
     5941 tcgagaggca tcaggtgctc gatgttctca ccaccgacaa catcgttgac aagaactact
     6001 acgaaacggt tttccccaca ctggagaaaa gcgatctcga tctgcgggtg atgtacgaga
     6061 tcaagtcgaa catcacaccg gagcaggcgc gcgcgttgcg cggcgcgggg gtcatccagg
     6121 tgcagccggg aatcgagaac ctccattcgc tgccgctgaa gctcatgcgc aagggcgtca
     6181 ccggcgcgaa caacgtcgcg acgcttcgcg aactccagga gcagagcctc accgtgctgt
     6241 ggaactacct cgtcggcttc cccggagaga ccgatgggtg ctatcaggag gtgatcaccc
     6301 agatccccct cctgtaccat cttcagccgc cgctcggcgc gcaccgcatc gcgctcgaac
     6361 gtttcaaccc gtacttcgac caccctgacc tgggattcgc cgagcgccgt ccgcgatcct
     6421 ggtaccgggg cgtgttcccc gacctgggcg aaacggacct gaacgacgtc gcctacctct
     6481 tcgagacgcc cgaccgaggc gtcggcggcg ccacgctggc cgggttgaag gcggcgttcg
     6541 acgcctggcg cgaggcgtac gcgacctcgg cgctcaccca ccgggccgtc gccgggcggc
     6601 tgcacctgta cgaccggcgg gccgggcggc gcgccgccga ccacgtcctg gacgagccga
     6661 ccgaggtcgc cgcctacgcc gcgctcgggc ggggccgctc gccggagggc ctcgcgcggc
     6721 acctgtcggc gggcgggtgg ccggtcgacg ccgcctgggc ctcggcgttc atcgacgatc
     6781 tcaccgcgct cggcctcgtc ttccgcgagt cggggcgcgc ggtgacgctg tcgaccgggc
     6841 acgacgcgac ggcggtgcgc ggcacggcca tccccacgcc cgccatggtc ggtgcgggca
     6901 catgatcctc cggggagcac gatgatccgc gatctgccgc cggtcgacct gaccagcgcc
     6961 gccggggtcg ccgccgcgcg agggcgcccg gacgtccgcg tcctggaggt gtccctcgcc
     7021 gacctggcgt ccggcacggc ggtcccgcgc gggaagcagg agccgctgac gtggctgcgc
     7081 gtccgcccga ccggggccga actcgacggc ggcccgtggc ccggcgacga cgcgctcggc
     7141 gcgctgcccg ccgccggggt cgtcggcctg acgctggagg ccccggcgct gccgcgcgcg
     7201 ccctggctga tcgccttcct cgtccgcgcg acctcgttcc agctcccgct ggagtggggc
     7261 ggcccggtcg cgggcctgcc gtgcgggctg ctgttccacc ttgcgccccc ggcgttcggc
     7321 gacgaggtcg cgggcaagtg gcgggcggcg caccgctacg gccagtgcta ctggcggcgc
     7381 gggcccgggt tcgccgccgt ccaggacctg cgcgaggacc cgggcgccca cttcgtcatc
     7441 cacgagccgg gcctgctcgc gctcttccac cgcctcgccg acccggtcga ggtcgcggac
     7501 ctcggcgcgg acgaccgcgc ccacctgcgc gacctgctgg acgcgcggct cgccgtcgag
     7561 ctcggcggcg tcgcggtcgg gctgccctac cggctgcgcc gctggcccgc gcccgtcatc
     7621 gacttctgac cggggaggag aaccggccga tcagcagcgc gagccgccgc tcgtggtcgg
     7681 cgcggcgcag gcggccgtgg cgcatcagca tgtgcaggcc gtgcaggccg ctccagaacg
     7741 tctcggtgag catctccagg tcgtcgtcgc ccgcgtaggg gcgcatggtc tcgcgcagtt
     7801 cgccgaaccc ctcccgcaag ggcgccggtg actccgggct ggcgaactcc aggtcgacgg
     7861 cgagcgtgag catcgcgtcg tacagcgcgg gccgccgctc gccgaactcg gcgtaggcgg
     7921 ccgcgaccgc cgcgatgccg cccggacggg ccgcgcgcag tacggcggcg aggtcggcta
     7981 acccctccag cgcgaccgcg gccatgatcg cgtccttgcc cgagaaatgg ctgtagagga
     8041 tgggctggct gtactcgatc tccgccgcga gccgccgcgt ggtcaccgcg tcccagccct
     8101 cggcctcggc cagctcccgc gcggccgtga cgatcgcccg ttcccgttcc gcccgctcgc
     8161 gctcccggcg cgcctgaatc gacatggcca tgattctagc atcgctagtc atcttgccaa
     8221 tgctctgcta acgtcgatca cgttcctagc actgctaact caacgggagt cgtcatgctc
     8281 gcaccgatcg cctacggcct ggccgtcctg ctcagcctgt tcgtcctgtt catcggcgcg
     8341 cggttcctcg tccagccgct tccggcggcg gccggctacg gcgtccccgc caagccggac
     8401 ggcgaccccg cctacctgag cgtcaagggg gtccgcgacg ccgccttcgg gctcctcggc
     8461 ctggccctgg tcgccttctc cggagccgac gccgccgcct ggtacatgct cgtcgtcgca
     8521 ctcgtcccgc tcggcgacac cgtgatcgtg cttcgcaacg gcgggacgag ggccgccgcg
     8581 ttcggcatcc acttcgcgac cgcgctggtc gtcctgctca gcgccgccct gctgttcgcg
     8641 gtctgagcga tggacgccgt cccggggctt ttcgaaacct ccccgcaccg cttgaatctt
     8701 gggcgggggc cgtcacgacc ggaggagggg ctgtggacga tctgcgaccc ggggaccccc
     8761 gtgccgtcgg ccggtacacc ctgacggcgc gcctcggcgc gggcgggatg ggccaggtct
     8821 acctcgggtc atcgcccggc ggacggcccg tcgccgtcaa gctcgtccac gccggtttcg
     8881 ccgccgacgc cgagttccgg cgccggttca agcgggaggt ggaggcggcc cggcgggtcg
     8941 gcgggttcca caccgcgccg gtcgtggacg ccgaccccga cgccgacacg ccctggctgg
     9001 tcaccgccta cgtccccggg ccctcgctgg cctcggcggt cgccgagcac ggaccattgc
     9061 ccccggcctc ggcccgggtg ctcggcgccg ggctggccga ggcgctggag gccgtccacg
     9121 gggcgggggt cgtgcaccgc gacctcaagc cgtccaacgt gctgatcgcc gccgacgggc
     9181 cgcgcgtcat cgacttcggc atcgcccgcg cggtcgacgc gtccgggatc acgacccgcg
     9241 ccgggacgcc cggcttcatg gcccccgagc tgatcacgga cggcgcgagc accccggcct
     9301 gcgacgtgtt cgcgctcggc gccgtgctcg ccttcgcggt cggggtgcgg ccgttcggcg
     9361 aggggccctc ggaggcgctg acctaccggg tcgtgcaccg ggagcccgat ctcggtggcg
     9421 tgccggagcc gctcggcccc ctggtcgcgg cctgcctcgc caaggacccg gcggcccgtc
     9481 cggcgccgtc cgacctgctc ggcgccctga ccccgggcgg ggacgcgggc gcctggctgc
     9541 cgccgcccgt ccaggagatg gtcacccgct tccccaccgc gcacacgccc actcccaggc
     9601 cggcaccgac gcgccaactc ccgtcgccgc aggccgcccc acccgcgcgg caggccgtgc
     9661 ggatcgcggg gcggatcacg cccgccgcga tcgcgctcgc cgcgtccgtc ctctactcgc
     9721 tgatctgggt cgcgatcttt ctgctcggcg ccgggctcgc cctcggcggc acctcctccg
     9781 acgccgacag cgtcgccacg ctcatcggcg tcgtcgtggc catagtcggc gcggtgaact
     9841 gcgtcagggc gattccgcgc gcggtctggc aggccatggc gctggcgcgg cccgtgacgt
     9901 tcgagatcgg ccccggcggg ctcagcctgc ggcacggcgg acgccgcgtc cactacgggt
     9961 ggcacgagat cggacgggtc gtgctccgcc gcatcgacga caagaagaag cgggcgttgt
    10021 gcgtatccct gaaccctggg agcgcgcagc ccgactacct gagcccgcgg agcccgttct
    10081 gctacaccga ggcggacacc ggctgggtcg tcatcgcccc gctgatccgg ctgcggacct
    10141 cgcggcggga gatcgagggg atcgtcgtcc ggtatgcggg cccccggtgg ggcggccacg
    10201 cctgacgcgc taggacgcca gcgtcccgca gtggtcgggc gacatgctcg cggccagcgc
    10261 ggcggcctcg ccccggtcgc gcagcccgag cttcccgagg acgttcgaca cgtggtactt
    10321 cacggtcttc tcgctcagcg cgagccgctc ggcgatccac gcgttggacg gacgggacgc
    10381 cagcagcgcc agcacctccc gctcgcgcgg cgtcagcaca ccgggacggt gcgggtcggc
    10441 ctcgaacagc tcgtccagca gcgcgggccc gtcgacgggg tcgtccagca ggcagcaggc
    10501 gaacgcgggg gcgaggtagc ggccgccggc ggcggccgtc cgggcggcgg cggtcacctc
    10561 ggcgaccggc ccccgccggt cggcgatgct cgcgacaccg gcccacagcg cggccagcag
    10621 ttcggggcgc ccggcggcct cgccgaaccg cgcggtgtgg tccaccagca gcacgaccgg
    10681 gacgcggagc acgcgcccct cccggcacac cgctcggacg aggtccccgt gcccggcccg
    10741 ccacgggctg aggtcgacca cgagcaggtc ggtcgcggcg gcgacgagcg cggcggccac
    10801 cgatctcccg tccggcacca cgagcctgac ctggcccccg agcgtccggc cgaggacgcc
    10861 cgccacccgg tcgcggcagt cggcgttggc tgagcacagc agtatcgacg tcacgtcctt
    10921 cacctcccgg cgcctcggcg aacgggaccc gatcacccga tcacctctgc cgcgagcctc
    10981 gccgatgggg ttacggacgc acatccggag aatcactgat attgccgatt cccggggtta
    11041 cgtagtgccc ggcggccgca ccccaggagc gccgcgaccg cggctcagga gggggacctg
    11101 agcgcgctga cgacgcccgc gcgggacgag acgcccagct tggcgaagat gtgcgcgagg
    11161 tggctctcga cggtgcgggc gctgatgaac agcgcctcgg cgatctgctg gttggtccgc
    11221 ccggccgaga ccagctcggc cacctccagc tcgcgccgcg acagctcgtg cgggccggac
    11281 gagcggccgc gcaccgggac gtagatcccg agccgccgct gctcccgcac cgcccgccgc
    11341 aggaacgagg ccgcgccgca gccgccgaac acctccgccg cgcgccgcag gtcggccttg
    11401 gcggtgtcgc ggcgggacgc gcgcgcggcg gcggtcccgg cggtgagcag cgcccgcccg
    11461 gcgtcgagcg ggtactcgtt gcccgccagc acggcggcgg cgccccgggc gtgctcggcg
    11521 gcggcgcccg ggtcggtgct aagcagggag tgcgcgcggg cgagcgcggc gaggccggtc
    11581 gagacgggca ggctcgcgtg ggagtacttg tcggcctcgg ccgcccactc ggcggacgcg
    11641 gcgtgccgcc cccgcgcggc ctccagctcg gcgagccgct cggagacctg gaccagcgtg
    11701 acgaggtcgg acccgggacg ggcgaacccg ttggccgcct ccagcaggga ccgggccccg
    11761 gcctcggcct gccccgtctc caccagcgcc aggccgagcg ccagctcggc ctgcttgccc
    11821 caccactcgc cctggtcgcc ggcggtgtcc acggcccgct gcgcgaagtc gaccgcgtcg
    11881 gcgccgttcc ccttccagta ctcga
//
