LOCUS       NZ_CP070976            12184 bp    DNA     circular CON 26-MAY-2024
DEFINITION  Bacillus halotolerans strain MBH1 chromosome, complete genome.
ACCESSION   NZ_CP070976
VERSION     NZ_CP070976.1
KEYWORDS    RefSeq.
SOURCE      Bacillus halotolerans
  ORGANISM  Bacillus halotolerans
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 4225863)
  AUTHORS   Plymale,R.C., Jones,M.E. and Hardin,B.R.
  TITLE     Antibiotic-producing soil bacterium Bacillus halotolerans MBH1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4225863)
  AUTHORS   Plymale,R.C., Jones,M.E. and Hardin,B.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2021) Biology, Ouachita Baptist University, 410
            Ouachita Street, Arkadelphia, AR 71998, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: MBH1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 604.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018417515.1-RS_2024_05_26
            Annotation Date                   :: 05/26/2024 04:50:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,253
            Genes (coding)                    :: 4,121
            CDSs (with protein)               :: 4,121
            Genes (RNA)                       :: 121
            rRNAs                             :: 10, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 84 of 132
            Pseudo Genes (incomplete)         :: 71 of 132
            Pseudo Genes (internal stop)      :: 24 of 132
            Pseudo Genes (multiple problems)  :: 41 of 132
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:46:13
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4086671
            Orig. end                         :: 4098855
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070976.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Ruth Plymale, Ouachita
            Baptist University, 410 Ouachita Street, Arkadelphia, AR 71998.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12184
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="10"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12184
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12184
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7184](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7184
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    124..139
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.72"
                     /tool="antismash"
     misc_feature    complement(698..713)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.91"
                     /tool="antismash"
     gene            complement(1953..2204)
                     /locus_tag="JYG31_RS20990"
                     /old_locus_tag="JYG31_20990"
     CDS             complement(1953..2204)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391862.1"
                     /locus_tag="JYG31_RS20990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_20990"
                     /product="hypothetical protein"
                     /protein_id="WP_024123458.1"
                     /transl_table=11
                     /translation="MKIQHKKELKFYCLITIPAAFAVLTVISFLLQEITFPVTASAFLN
                     ASWHNLLFLIPFCLFFYPVHIWMKREFSRWNDAGQKRG"
     gene            2323..3144
                     /locus_tag="JYG31_RS20995"
                     /old_locus_tag="JYG31_20995"
     CDS             2323..3144
                     /NRPS_PKS="Domain: PKS_KR (7-160). E-value: 2.9e-10. Score:
                     32.3. Matches aSDomain:
                     nrpspksdomains_JYG31_RS20995_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391863.2"
                     /locus_tag="JYG31_RS20995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_20995"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_213418166.1"
                     /sec_met_domain="adh_short (E-value: 4.2e-33, bitscore:
                     112.8, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 2.7e-38, bitscore:
                     130.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGRLDNKTAVITGAATGIGQATAEVFANEGARVIVGDMNEDQMKE
                     TVEAIRQNGGQAEAFHLDVSDEENVKQFADQLKDAYGTIDILFNNAGVDQEGGKVHEYP
                     VDLFDRIIAVDLRGTFLCSKYLIPLMLEKGGSIINTSSMSGRAADLDRSGYNAAKGGIT
                     NLTRAMAIDYARNGIRVNSISPGTIETPLIDKLAGTREQEMGEKFREANKWITPLGRLG
                     KPKEMATVALFLASDDSSYVTGEDITADGGIMAYTWPGKMLIDEKWKEETK"
     aSDomain        2344..2802
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JYG31_RS20995_PKS_KR.1"
                     /evalue="2.90E-10"
                     /label="JYG31_RS20995_PKS_KR.1"
                     /locus_tag="JYG31_RS20995"
                     /protein_end="160"
                     /protein_start="7"
                     /score="32.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="TAVITGAATGIGQATAEVFANEGARVIVGDMNEDQMKETVEAIRQ
                     NGGQAEAFHLDVSDEENVKQFADQLKDAYGTIDILFNNAGVDQEGGKVHEYPVDLFDRI
                     IAVDLRGTFLCSKYLIPLMLEKGGSIINTSSMSGRAADLDRSGYNAAKG"
     CDS_motif       2350..2427
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JYG31_RS20995_0001"
                     /evalue="1.30E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="JYG31_RS20995"
                     /protein_end="35"
                     /protein_start="9"
                     /score="17.8"
                     /tool="antismash"
                     /translation="VITGAATGIGQATAEVFANEGARVIV"
     gene            complement(3292..3516)
                     /locus_tag="JYG31_RS21000"
                     /old_locus_tag="JYG31_21000"
     CDS             complement(3292..3516)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021923.4"
                     /locus_tag="JYG31_RS21000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_21000"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_024123460.1"
                     /transl_table=11
                     /translation="MENNNRLSELNVKEMEQIDGGGWRSALGGAFSGALVGASTVGVGG
                     AAVGGPVGGAGGAITGGIVGGIVGGINAW"
     gene            complement(3533..3763)
                     /locus_tag="JYG31_RS21005"
                     /old_locus_tag="JYG31_21005"
     CDS             complement(3533..3763)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JYG31_RS21005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JYG31_21005"
                     /product="hypothetical protein"
                     /protein_id="WP_024123461.1"
                     /transl_table=11
                     /translation="MNAKSIKDVELQALTLEEAEQIDGGSYAKYLGSAVVTGAGAGVVG
                     GVPGVILGAHAGAIAGSVGYLGGSLIAGKWL"
     misc_feature    complement(3909..3927)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 24.61"
                     /tool="antismash"
     misc_feature    3937..3955
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: medium, score: 26.61"
                     /tool="antismash"
     gene            complement(3956..4381)
                     /locus_tag="JYG31_RS21010"
                     /old_locus_tag="JYG31_21010"
     CDS             complement(3956..4381)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF042802.2"
                     /locus_tag="JYG31_RS21010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_21010"
                     /product="DUF6773 family protein"
                     /protein_id="WP_024123462.1"
                     /transl_table=11
                     /translation="MKDERISHIENKIGHESFLLVIALVFVSFILSIMFKMPSTLMTII
                     ILFVGSGYYGLRAMLKGILRVNLKKKKKSSYFLLIVFTFILYMATSVLFKIITFPFTTK
                     EFLFQILMYILWLIPFSLIMYTTYKFSNRQQKKELEN"
     gene            complement(4371..4589)
                     /locus_tag="JYG31_RS21015"
                     /old_locus_tag="JYG31_21015"
     CDS             complement(4371..4589)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018664240.1"
                     /locus_tag="JYG31_RS21015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_21015"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_024123463.1"
                     /transl_table=11
                     /translation="MNNNLKLERTRANLTQEQLAEKIGVTRQTIGLIEKGKYNPSLQLC
                     LKICIELKVTLNDVFWTHNREEETNEG"
     misc_feature    4788..4803
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.73"
                     /tool="antismash"
     gene            5001..7184
                     /locus_tag="JYG31_RS21020"
                     /old_locus_tag="JYG31_21020"
     CDS             5001..7184
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014481436.1"
                     /locus_tag="JYG31_RS21020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_21020"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_213418167.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 8.6e-43, bitscore:
                     143.3, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 3.9e-236, bitscore:
                     783.8, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFFNRYHCVRQLDQKDCGAACLSTIAKHYGSKLSLAYIRDIAGTD
                     TQGTSALGMVKAAEQLGFSAKAVRAEHTAFDQSITLPAIAHIIIDQQLLHYVVIHKISK
                     DKIIIADPGKGLVTYQKDDFFKIWTGILILLAPTETYKKQTETSNISADIFRLILQQKS
                     LIIHTFLASFIITVFGILGTFYFQTIIDQILPNGLSNTLHILSIGLLIMYLFKVFLIGF
                     RQYLMILLGQKLSISIMFGYFQHVLTLPMKFFSNRKVGEIISRFTDANKVIDAVVSASL
                     SVILDTIMLIMVGIFLYLQNTTLFLITLAFIPFYILTVWIFIKPYENINNTEMENNAQL
                     TSKIVETLNGIETVKAYNAEYMMSFETEKRFVKYLRSAFKHGVIDNLQSSIKMFLDLAS
                     GALILWIGSTQVMKGTLTIGQLITYNALLTYFLNPLQNIIGLQSKLQSASVASKRLGEI
                     LKLEPELIEKEARITSTSSITGPLSFKKINFRYGTRRLILKDITLHIQKGEKVAFVGES
                     GSGKSTLTKLLMKFYTAESGEVSINGYHINDIHINALRESISYIPQESHFIQGSILENL
                     LLGNSGSFTFEEIIEVCKQTKVHDFINDLPMRYDTLVEENGSNLSGGQKQRLAIARALL
                     RKPNILIMDESTSNLDTTTEQGISKMIYDQTVDVTTIIIAHRLSTIMNCDKIFVMKNGE
                     IIEYGNHNDLLKKKGKYYELWENQMPSREMVTI"
     gene            7185..8654
                     /locus_tag="JYG31_RS21025"
                     /old_locus_tag="JYG31_21025"
     CDS             7185..8654
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014481437.1"
                     /locus_tag="JYG31_RS21025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_21025"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_024123466.1"
                     /transl_table=11
                     /translation="MKRNFFNLNDLRDSREFYESKPKPIGVFFTYILIALLIACIVFSF
                     YGKKEVILKTTADIQYTKPNSNVTNEVAGKVKTVDIKQVDKVKKGDTLLTLDNTEIQAQ
                     KKAVKEDIKDLKEQAALNKTLKKSIENDKNLFSENDHWGYQDQYLSYEKQSNLLESSKE
                     NAKTQQKKLKKQKISIKNSLDKTKNKVKLKLQRISDLQDSIKNNKSFAKQDDKYNSYYN
                     SYQTNKQNVEQQLDDLRKMKDGLDKNSFSQQKENLNGQLESLKQDMLVKLSEEKDELNQ
                     SLDEINSSMKSDDSDSDSDNSEETNYKLSIEQLKTNLLNQTSQKQTDLQTTLKSKEDDL
                     KMIDLQLEKYKIKATSDGYVNVLTNISPGDNLQAGTAILTIVPDKEKLRANIYIPSHEI
                     TNINEKDEVRFHIENTNQTIKGVIIKISPDSISDQNGQKFYIAESKIESKEIVKNNGTK
                     IHLKNGMQTDVSIIVDSKRYIDYFLDTLNLH"
     gene            complement(8975..10117)
                     /locus_tag="JYG31_RS21030"
                     /old_locus_tag="JYG31_21030"
     CDS             complement(8975..10117)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391864.1"
                     /locus_tag="JYG31_RS21030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_21030"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_213418168.1"
                     /transl_table=11
                     /translation="MPESAEAQIKQALLDLLEKRDIRQITMKEIAEHAQVSRGTLYLYY
                     EDKFSIIEDVIVDMKEGLGEALYNAFKHIDTFHLNKKRHTVHPTLSFVHEHRSFFGTMM
                     NRGQFHRFFKDVFQQDVLLAPIHVNLTPIEREIYGHYRALYTYAIILYWLNEDAEASPE
                     AISQKVWELVSQKRFYWLFGQAPSWERQEDKQIDRRVVRTREALQKALLDVLSEKKDYA
                     AITISDITRKSNIRRATFYDHYASKEELLQTIIQQACAEIIDHLSIAFSPNELSLEEAR
                     EALASLLSALDRMPIVHLLNRDWGVPHVIPEMLKALESFYLHQQTNIPAEKKLYAYYVS
                     AMIIGLILYRLDEGKAYAPEVLTREFLQFLDVKKYKVVLL"
     misc_feature    10078..10091
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 20.38"
                     /tool="antismash"
     gene            10259..11596
                     /locus_tag="JYG31_RS21035"
                     /old_locus_tag="JYG31_21035"
     CDS             10259..11596
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391865.2"
                     /locus_tag="JYG31_RS21035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_21035"
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_213418169.1"
                     /sec_met_domain="Aldedh (E-value: 3e-97, bitscore: 324.0,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQQVKDDIQRVFQLQKKQQKVLRASTAEQRKEKLQRFLDSVIAH
                     EEEIIEAIRKDVRKPYHEVKKAEIEGTKKAIRDNMNNLETWMAPKEVGSSLSSDANGIL
                     MYEPKGVTLILGPWNYPFMLTMAPLAASIAAGNSAIVKLSDFTMNTSNIAAKVIRDAFD
                     EKEVAIFEGEVEVATELLDQPFDHIFFTGSTNVGKIVMTAAAKHLSSVTLELGGKSPTI
                     IDSEYDLMDAAKKIAVGKFVNAGQTCIAPDYIFIKKDVQDKFAGILQTIVNAGFMEDDY
                     TPDRNKFTQIVNDRNFNRVRALYDDAIEKGAEVVFGGVFDASDRTISPAVLKNVTPDMK
                     IMQEEIFAPILPMMNYEDIDEVIDYVNDRDKPLALYVFSHNQDLIDNVLQHTTSGNAAI
                     NDVVVHFSDVNLPFGGVNTSGIGSYHGVYGFKEFSHEKGVFIQAAK"
     gene            complement(11719..12144)
                     /locus_tag="JYG31_RS21040"
                     /old_locus_tag="JYG31_21040"
     CDS             complement(11719..12144)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003219064.1"
                     /locus_tag="JYG31_RS21040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_21040"
                     /product="DUF5391 family protein"
                     /protein_id="WP_213418170.1"
                     /transl_table=11
                     /translation="MSRKNRVVIMTAISAFLFCAMIAAASLSPLADTGSSANQFNSIGM
                     WSAIGMILVFYLIPILIYMLGVDAMRYVMAALCGMGLLIHLSTAALVLMVSIFSDHPLS
                     DVITVMGLCLASAAVNIIWFFATFRSAPKKPVTRDFT"
ORIGIN
        1 tcttgttcga aagcgttctg cagcgtcttg aatacctcgt gattcgggtt gatttccagc
       61 actttgtccg cttttacatt ttggctgtcc ggcatcgcat ttaagatttt ttccatctcg
      121 attgtcactt caccgtcagc tgcaaggcag accggatgag atttgagacg tttcgatact
      181 ctgacgtttt tcactttgtc agacaggata tttttcattt cctcaaacag atccttgtac
      241 ttgctttcct cttcttcaga ctgcttttga tcctcttctg aatcgatgcc aagatcgccg
      301 cttgatacag atttgaactc tttttcttga tatgaagcga gcattttgat cgcaaattca
      361 tcgatgtcct ctgtaaagta aagaatttca tagcctttgt ccgctaccat ttccgtttgc
      421 ggcagttttt caatgcgatc gtatgaatca ccggtcgcgt agtagatata tttctgctct
      481 tcaggcattc tggatacgta ctcgtccagg ctgaccagtt ttttctcttt ggaagagtag
      541 aatagtaaga gatccttcaa ttgatcttta ttcgcgccaa aatcattgta cacgccgaac
      601 ttcagctgtc tgccgaacga tttgtagaag gtctcatatt tttctctgtc ttttttcagc
      661 aggctctgca gctcgctttt gatctttttg ctgatgtttt tcgcaatcag cttcagctgg
      721 cggtcatgct gcagcatttc tctggaaatg ttcagcgaca ggtcctcaga atcgaccata
      781 ccctttacaa agctgaagtg gtccggcagc agatccgcgc atttgttcat aatcagcacg
      841 ccgttggaat acagctccaa gcctttctca tattccttag agtaatagtc aaacggcatg
      901 ttttctggaa taaatagaat cgcgttgtag cgcacggcac catccacact gatatgaaca
      961 tgtgtgagcg gtttatcaaa tccgtaatgc ttttcagcgt agaacgtttc atagtcctca
     1021 tctgtcagct cgctcttgtt ttttctccag atcggcacca tgctgttaac cgtctgttcc
     1081 tcctgcacct cttcaaattc attgtcgctg ccttccttcg gcttattaac cgttgtatcc
     1141 attttgatcg ggtagcgaat gaagtcagag tactttttga tgatcgcttt taaacgatat
     1201 tcttctaaaa actcatcata gctctcatct tccgtgttct ctttgatctt caggataatg
     1261 tctgtgccga cagaatcctt ttcacacggc tcaattgtat agccgtctgc gccagcagat
     1321 tcccatttat acgcttcttc gctgccgagc gccttactga taacggtcac cacatcggca
     1381 accataaacg ccgcataaaa tccgacaccg aattgcccga tgatgtcatg gccgtccttc
     1441 agttcgtttt cttttttaaa cgcaagagag ccgcttttcg caatcgtacc gaggtgctgt
     1501 tcaagctcgt cctttgtcat tccgatccct gtatccgtga tggtcagtgt tctcgcgcct
     1561 ttgtcagccg cgacttttat gtagtaattg tctttatcga aggttagcgc atcatctgtc
     1621 agcgctttgt aatagatttt gtcaatcgcg tcactcgcat tggaaattaa ctcgcgaaga
     1681 aagatttctt tttgagtgta aatggagtta atcatcatat ccagcagtcg tttggactct
     1741 gctttaaact cttttttagc cattcagctc gctccttttt catgctttgt gatattatgt
     1801 aaactctttt cagcatctat ttatcataaa tgacgactca tgtcaatacg attttcatca
     1861 aagatcaata gatgcatata tatgtaaatt taacattagt tgggggtgtt ttcactattt
     1921 tttctagaca gtctgataag aaaaagagag gatcagcctc tcttttgtcc tgcatcattc
     1981 catcggctaa actcccgctt catccaaata tgcaccggat agaaaaacaa acagaacggt
     2041 atcagaaaca atagattatg ccatgaagca ttcagaaatg cgctagctgt aaccggaaac
     2101 gtaatctctt gcaataaaaa agagatgaca gtcagcacag caaacgcagc cggaatcgta
     2161 atgagacaat aaaatttaag ttccttctta tgctgtatct tcaaaacaca taaaacctcc
     2221 caacaatttc tagttcattt taacatatgc aggcatttct tttctcacat gtttatcact
     2281 gcacacagcg ggaagacaac tagaaaaagg aggattcgtg ttatgggaag acttgataac
     2341 aaaaccgctg tgatcacagg cgctgcgaca ggcattggac aagcaacggc agaggttttt
     2401 gccaatgaag gcgcgcgtgt gattgtcggc gatatgaatg aagatcaaat gaaagagaca
     2461 gtcgaagcga tcagacaaaa cggaggacag gctgaggctt ttcaccttga tgtgtcagat
     2521 gaagaaaacg taaagcaatt tgctgatcaa ctcaaagacg cgtacggaac gattgatatt
     2581 ctctttaaca acgccggcgt cgatcaagaa ggcggaaaag tgcacgaata tcccgtagat
     2641 ctgtttgacc gcataatcgc agtcgatctg cgcggcacgt ttctttgcag caaatatctg
     2701 attccgctta tgcttgaaaa gggaggctcc atcatcaaca cctcctccat gtcaggcagg
     2761 gctgcggacc ttgaccgctc cggctataac gccgcaaaag gcggaatcac aaacctgaca
     2821 agagcgatgg caattgatta cgcaagaaac ggcatccggg tcaattccat ctcaccgggt
     2881 acgattgaaa cgccgcttat tgacaaatta gctggcacca gggaacagga aatgggcgag
     2941 aaattccgcg aagccaacaa atggatcaca ccgctcggcc gcctcggcaa accgaaggaa
     3001 atggcgacgg tggcgctgtt cctcgcttct gacgacagct catacgtcac aggcgaagac
     3061 atcaccgcag acggcggcat catggcgtat acatggcctg gcaagatgct gattgatgag
     3121 aaatggaagg aagaaacaaa ataactgaga tagtcagtta aaagaattta taataaatag
     3181 acgctacaaa acattatgta tatataaata caaatataac aagaccgaga aaagatatac
     3241 tctttccggt cttgttacat aatgttcaaa ctagttcttt atgtattttt attaccaagc
     3301 gtttattccg ccaactatac cgccaactat gccgccagta attgctccgc cggcaccacc
     3361 aacgggtccg ccaacagctg ctccaccgac gcctactgtt gaagcaccga ctaatgcacc
     3421 tgaaaaagct ccgccaaggg cacttctcca gcctccgcca tcaatttgtt ccatttcttt
     3481 tacattcaat tctgaaagac gattattatt ttccatttat gctattccct ccttaaagcc
     3541 attttcctgc gattaatgac ccgcctaaat aaccaactga ccctgcaatt gcacctgcat
     3601 gggcaccaag aataacacct ggaactcctc cgactacacc tgcgccagca cctgtaacta
     3661 ctgcactgcc aagatattta gcataacttc ctccatcaat ttgttcagct tcttcaagag
     3721 tcaatgcctg aagttcaacg tcttttatgc tttttgcgtt catcaatatt cctcctcaaa
     3781 gttattcagg taaataataa taactagtat aaatttacca tgggaattat aatattacat
     3841 aataaatcct aaatcaatag ttttttccat aaaatcatct ataacctatc gatcaattta
     3901 tatatagtaa aagagagctt aacctaattc agttatttag gttaagctct cttaattaat
     3961 tctctaattc ttttttctgc tgcctgtttg aaaatttata tgtagtatac atgatgagac
     4021 taaaaggaat tagccataaa atatacataa gaatttggaa taagaattct ttagttgtaa
     4081 aagggaatgt aatgatttta aataataccg acgttgccat atacagaata aaagtaaaaa
     4141 caattaaaag aaagtacgaa ctcttcttct tctttttcag atttaccctt aaaatgcctt
     4201 ttaacatagc tcttaatcca taatatccgc ttccaacaaa caatatgata attgtcatta
     4261 acgtgctcgg cattttaaac ataatcgaaa gtataaaaga gacaaaaacc aaagcaatta
     4321 ctaaaagaaa gctttcatga cctattttat tttcaatatg acttattctc tcatccttca
     4381 ttagtttctt cctccctgtt atgcgtccaa aatacatcat tcagagtaac tttcaattct
     4441 atacaaattt tcaaacataa ttgtaaagaa ggattatact tccctttttc aattaatcca
     4501 atcgtctgtc ttgtcactcc aattttttcg gcaagttgtt cctgtgttaa attcgctctt
     4561 gttctttcca acttcaaatt attattcatt ggcaagtctc cattggttta tttatctgta
     4621 gcatatcata tataaaaaac ccatgcaaac tatatattgc atttatccat atatatttta
     4681 cattttgtct attatatctt acaatgaatt ttaacattta taggagttgg taaaatgaaa
     4741 ttgttctata tctttataat cgcagggggt ttattttgta tatctctttc ttattttttt
     4801 cacttaggtg tatttaatct tgctttatat cttatctata tcaattacgc atttatatta
     4861 tataaacgtg ccaaaagctc taaataacaa taaatattta ccttaaaaag gttgacaaac
     4921 catgaaaatg taataaaatg atgtctattc tttatattat atatattcta aaaaatgacc
     4981 ttcattatgg agagtagtat atgtttttta atagatatca ttgtgtacga caacttgatc
     5041 aaaaagattg tggagcagcc tgcctatcaa caattgcaaa acattatggc tcaaagctct
     5101 ctctagctta tatacgtgac attgctggaa ctgatacaca aggtactagt gctcttggaa
     5161 tggtaaaagc cgccgaacag ttggggtttt cagcaaaagc tgtgcgtgca gagcatacag
     5221 cttttgatca gtccattact cttccagcaa ttgcacatat tattatagat caacaattat
     5281 tgcattacgt tgttatacat aaaattagta aagataaaat tatcattgcg gatcctggaa
     5341 aagggctcgt tacatatcaa aaagatgatt tttttaagat ttggacaggc atcttaatac
     5401 ttttagcacc tactgagact tataaaaaac agaccgagac aagtaatata agtgccgata
     5461 ttttcaggtt gattcttcag caaaaatcgt tgattataca tacgttttta gcatccttta
     5521 ttattaccgt cttcgggatc ctcggtactt tttactttca gacgatcatc gatcaaatcc
     5581 tacctaatgg attaagtaac acattacata ttctttcaat cggccttttg attatgtatt
     5641 tatttaaggt tttcttaata ggttttaggc aatacctaat gattctccta ggtcaaaaat
     5701 taagtatctc catcatgttt ggatactttc aacatgtgct tactttgcca atgaaatttt
     5761 tcagcaatag aaaagtcggc gaaattatat ccagatttac tgatgctaat aaagtgatcg
     5821 atgcggttgt cagtgcatcg ctttcagtca tactcgatac cattatgctt attatggttg
     5881 gtattttctt atatctgcag aatactactt tatttctcat cacattggct ttcatacctt
     5941 tttatatttt gacggtttgg atttttataa aaccctacga aaatataaat aacacagaaa
     6001 tggaaaacaa tgcgcagctg acttcaaaga ttgttgagac gcttaacggc atcgaaactg
     6061 ttaaagcata caatgcggaa tatatgatgt catttgaaac agaaaaaaga tttgtgaaat
     6121 acttacgatc agcttttaaa cacggagtta ttgataacct tcagagttcc ataaaaatgt
     6181 ttttggattt agcaagcggt gcattgatac tgtggatcgg atcaactcag gtcatgaaag
     6241 gcacccttac tatcggtcag cttattacat ataatgcctt attaacctac ttcttaaatc
     6301 ctttacaaaa catcatcggc ctacaatcta agcttcagtc agcttctgtt gcgtccaaaa
     6361 ggctcggaga aattctaaag ctagaacccg aattaataga gaaagaggcg cgaataacat
     6421 cgacgtcgtc aattacaggt ccgctttctt ttaaaaaaat caattttcga tacggaactc
     6481 gaaggctcat cttaaaagat attacactgc atattcaaaa aggagagaaa gttgcctttg
     6541 ttggagaaag cggttcagga aaatctactt tgactaagct tttaatgaaa ttttatacag
     6601 ctgaatcagg tgaggtttca atcaatggtt atcatataaa tgatattcac atcaatgcgt
     6661 taagggaaag cataagttat atacctcaag aatctcattt tatacaagga tcaattcttg
     6721 aaaacctttt gcttgggaac tcaggcagtt ttacctttga agagattata gaagtatgta
     6781 aacaaactaa agtacatgat tttatcaatg atttaccaat gagatacgat actcttgtag
     6841 aagaaaacgg gtctaattta tctggaggcc agaagcaaag actcgcaatt gccagagcat
     6901 tattaagaaa acctaacatt ctcataatgg atgaatcaac tagcaatctc gatacgacaa
     6961 ccgaacaggg aatatcaaag atgatatacg atcagactgt tgatgtaact acgattataa
     7021 ttgctcatcg tttgagtaca attatgaatt gcgacaaaat atttgtcatg aaaaatggag
     7081 aaatcataga gtatgggaac cacaatgatt tattgaagaa aaaaggcaag tactatgagt
     7141 tgtgggagaa ccaaatgcca tcgagagaga tggtgacaat ttagatgaaa cgtaactttt
     7201 tcaatttaaa tgatttgagg gatagcagag aattctacga atcaaaaccc aaaccaattg
     7261 gtgttttctt tacatatata ctgatagctt tacttattgc atgtattgtt tttagtttct
     7321 atggaaagaa agaagtgatt ctaaaaacga cagctgacat ccaatatacc aagcctaaca
     7381 gcaacgtaac aaacgaagta gccggcaaag taaaaacagt tgatattaaa caggttgata
     7441 aggtgaaaaa gggagatact ctattaactc tagacaatac tgaaatccaa gcgcagaaaa
     7501 aagctgttaa agaagatatc aaagatttaa aagaacaagc tgcgttaaac aaaactctaa
     7561 aaaaaagtat tgaaaatgac aaaaatttat tcagcgagaa cgatcattgg ggttatcaag
     7621 atcaatatct ctcatatgaa aaacaaagta atttgttaga gtcatcaaaa gaaaatgcaa
     7681 aaactcagca gaaaaaactt aagaagcaga aaataagcat aaaaaatagt ctggataaga
     7741 caaaaaacaa agtgaaacta aagcttcaaa gaatttctga tctgcaagac tccattaaaa
     7801 acaataaatc ctttgccaaa caggatgata agtacaattc ttactataat agttatcaaa
     7861 ctaacaaaca aaatgtagag cagcagcttg atgatctacg gaagatgaaa gatgggctcg
     7921 ataaaaacag tttctcacag cagaaagaga atctaaacgg acagcttgaa agtttaaaac
     7981 aagatatgct agtaaaacta agtgaagaaa aagacgaatt aaatcaatca cttgatgaga
     8041 ttaacagttc aatgaaaagt gacgattctg acagtgattc cgataactca gaggaaacaa
     8101 attataagct ttcaattgaa cagcttaaaa caaacttgct aaatcaaaca tctcaaaagc
     8161 aaacagatct tcagaccact cttaaatcaa aagaagatga tctaaagatg atagatttac
     8221 aattagaaaa atataaaata aaagcaacta gtgacggcta tgttaatgta ctaacaaata
     8281 tatctccagg tgacaattta caagcaggca ctgctatttt gactattgtc cctgataaag
     8341 agaagcttag agctaacatt tatattccga gtcatgagat cacaaatatt aatgaaaaag
     8401 atgaagtaag attccatatt gaaaatacaa atcaaaccat taaaggtgtc attatcaaaa
     8461 taagtccaga cagtattagt gatcaaaatg ggcaaaaatt ttatattgct gaaagcaaaa
     8521 tagaaagcaa agaaatagtt aaaaataacg gaacaaaaat acacttaaaa aatggcatgc
     8581 agactgatgt atctattatt gttgattcaa aaagatatat tgattatttt ttagatacat
     8641 taaacttaca ttaattccca tcaagagaag tgtgcatata tgcctcaaag cagatgagca
     8701 tctgctttga ggcattttat ttttttaaga aaggtagagg acgtatagaa tcatgtttca
     8761 aaacaaactg ttatttttac tctttttatt ctcttcaatc gttcgccttt tctttaatac
     8821 agtattgttc cccactgtaa gctggaagtc ttggacggtg aatattgttt ttattctttt
     8881 gtctcttcta tttctcaatc tcaccttaaa agaaagaaaa tcaaaagacc aagtatcata
     8941 acgacctaaa gcccggatta agtctccggg cacttcaaag caatacaacc ttatacttct
     9001 tcacatccaa aaactgcaaa aactccctcg tcagcacttc cggcgcatac gccttccctt
     9061 catcgagccg atacaatatc agcccgataa tcatcgccga tacataatag gcataaagct
     9121 ttttctccgc aggaatatta gtttgctgat gcagataaaa tgattccaac gctttgagca
     9181 tctcgggtat cacatgagga acaccccagt cccgattgag caaatggaca ataggcatgc
     9241 ggtctaaagc agaaagcagg ctggcgagcg cctccctcgc ttcctctaaa gaaagctcat
     9301 tcggactgaa ggcaatcgac aggtgatcta tgatctccgc acacgcttgc tgaatgatgg
     9361 tctgcagcaa ttcttcttta ctggcatagt gatcataaaa ggttgcgcgg cggatattgc
     9421 ttttccgggt aatgtcagaa atcgtgatgg ccgcgtagtc ttttttctct gacaatacgt
     9481 ccagcaaagc cttctgcagc gcttctctcg ttcgaaccac acgcctgtcg atttgcttgt
     9541 cctcctgccg ctcccatgac ggagcctgtc caaacagcca ataaaaccgc ttttgagaaa
     9601 cgagctccca tactttttgg ctgatggctt ccggagacgc ctccgcatcc tcattcagcc
     9661 aatacaggat aatcgcgtac gtgtacaagg cccggtaatg tccgtaaatt tcccgttcga
     9721 tcggcgtcaa attcacatga atcggtgcca gcaacacatc ctgctgaaac acatccttaa
     9781 aaaaacgatg aaactgtcct ctgttcatca tcgtgccaaa aaaggatcga tgctcgtgga
     9841 caaaagacaa agtcggatgc acggtgtgcc gctttttatt taaatggaat gtgtctatat
     9901 gcttgaaggc gttatagagc gcctcgccca gaccttcctt catgtctacg attacatcct
     9961 caataatgga gaacttgtct tcataataaa gatagagtgt cccgcggctc acttgtgcgt
    10021 gttcagcaat ttctttcatt gtaatctgtc gaatatccct tttttctaat aaatccagaa
    10081 gcgcctgttt gatttgagcc tcagcagatt ccggcaaaag agtcacctcc aaacttagac
    10141 agatgttcga tatatgtacg gtaacgtata gatgattctc cgttgtcttt cttatagaaa
    10201 agataccact ttataatagt tccgagataa gcagaaagca aatgaggggg attttacaat
    10261 ggaacagcaa gtaaaagacg acattcagcg cgtcttccag cttcagaaaa aacagcaaaa
    10321 agtgctgcgc gcttcaacag cggaacagcg gaaagaaaag cttcagcgct ttttggattc
    10381 cgtcattgca catgaagagg aaatcatcga agcgatccgc aaagacgtgc ggaagccata
    10441 tcacgaggtc aaaaaagcgg aaatcgaagg tacaaaaaaa gcgatcagag ataatatgaa
    10501 caacctggaa acatggatgg cacccaaaga agtcggttca tccttaagct ctgatgccaa
    10561 cggaatcctt atgtacgagc ctaaaggcgt cacgcttata ctcggtccgt ggaactaccc
    10621 gtttatgctg acgatggcgc cgcttgccgc ttctatcgct gcaggaaaca gcgccattgt
    10681 gaagctgtca gactttacga tgaacacgag caatattgcc gcaaaagtca tccgagatgc
    10741 ttttgacgaa aaagaagttg ccatttttga aggagaggtt gaagtggcca ctgagcttct
    10801 tgatcagccg ttcgaccaca ttttcttcac cggaagcaca aatgtcggaa aaatcgtcat
    10861 gacagccgcc gccaaacact tgtcatctgt cacgcttgag cttggcggca agagtccgac
    10921 catcatcgac agtgaatacg acttaatgga cgcagcgaaa aaaattgctg tcggcaagtt
    10981 cgtaaatgcc ggccaaacct gcatcgcacc ggattatatc ttcattaaaa aagacgtgca
    11041 ggacaagttc gcaggcattt tacaaaccat tgtcaacgca ggatttatgg aagatgatta
    11101 cacgccggat cgaaacaaat ttacgcaaat tgtcaatgac cgcaacttta accgcgtcag
    11161 agctttatac gacgatgcca tcgagaaagg cgccgaagtc gtgttcggcg gcgtatttga
    11221 tgcaagcgac cgtacgatct cgccagccgt tttaaaaaac gttacgccag atatgaagat
    11281 catgcaggaa gaaatctttg caccgatcct gccgatgatg aactatgaag acatcgatga
    11341 ggtcattgat tacgtaaatg accgcgataa accgctcgcg ctttatgtat tcagccacaa
    11401 ccaagacctg atcgacaacg tccttcagca caccacatca ggaaatgcag cgatcaatga
    11461 tgtcgtcgtt catttcagtg acgtcaacct gccattcggc ggcgtaaata caagcggaat
    11521 cggctcttac catggggtat acggatttaa agagttttct catgaaaaag gtgtgtttat
    11581 tcaagcagct aaataaacat gatttagaga atctgcatca tcttgcaggt tctctttttc
    11641 tatcatcccc gacaaagttt cataacaata aataaatgta gttcacagaa aaatcatgca
    11701 ttaacacaaa atagaaaact atgtaaaatc ccttgtgaca ggtttctttg gcgcagaacg
    11761 aaacgttgca aaaaaccaaa tgatgtttac agccgctgaa gcgagacaga gccccatcac
    11821 agtgatcaca tcagagagcg gatggtcact gaatatactg accatcagca caagggcggc
    11881 agtagataag tgaatcagca aacccatgcc gcaaagcgcc gccatcacat atctcattgc
    11941 atccacaccc agcatgtaaa tgaggatagg aatgagatag aaaacaagta tcataccaat
    12001 tgccgaccac ataccgatag agttaaactg attggctgag gaccccgtgt ctgccagcgg
    12061 agaaagtgaa gccgcagcaa tcatagcgca aaaaaggaat gcagaaatcg cagtcatgat
    12121 caccacgcga ttttttctgg acatgatcaa ttgacacctc ctaaaataga aatattaacc
    12181 ttta
//
