LOCUS       NZ_CP070976            21613 bp    DNA     circular CON 26-MAY-2024
DEFINITION  Bacillus halotolerans strain MBH1 chromosome, complete genome.
ACCESSION   NZ_CP070976
VERSION     NZ_CP070976.1
KEYWORDS    RefSeq.
SOURCE      Bacillus halotolerans
  ORGANISM  Bacillus halotolerans
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 4225863)
  AUTHORS   Plymale,R.C., Jones,M.E. and Hardin,B.R.
  TITLE     Antibiotic-producing soil bacterium Bacillus halotolerans MBH1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4225863)
  AUTHORS   Plymale,R.C., Jones,M.E. and Hardin,B.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2021) Biology, Ouachita Baptist University, 410
            Ouachita Street, Arkadelphia, AR 71998, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: MBH1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 604.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018417515.1-RS_2024_05_26
            Annotation Date                   :: 05/26/2024 04:50:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,374
            CDSs (total)                      :: 4,253
            Genes (coding)                    :: 4,121
            CDSs (with protein)               :: 4,121
            Genes (RNA)                       :: 121
            rRNAs                             :: 10, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 84 of 132
            Pseudo Genes (incomplete)         :: 71 of 132
            Pseudo Genes (internal stop)      :: 24 of 132
            Pseudo Genes (multiple problems)  :: 41 of 132
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:46:13
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3816407
            Orig. end                         :: 3838020
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070976.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Ruth Plymale, Ouachita
            Baptist University, 410 Ouachita Street, Arkadelphia, AR 71998.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21613
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="sactipeptide"
                     /region_number="8"
                     /rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /tool="antismash"
     cand_cluster    1..21613
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /kind="single"
                     /product="sactipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21613
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11613](+)"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11613
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
     misc_feature    complement(140..154)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.21"
                     /tool="antismash"
     gene            complement(232..3918)
                     /locus_tag="JYG31_RS19640"
                     /old_locus_tag="JYG31_19640"
     CDS             complement(232..3918)
                     /GO_component="GO:0009325 - nitrate reductase complex
                     [Evidence IEA]"
                     /GO_function="GO:0008940 - nitrate reductase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1134: flavodoxin"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391609.2"
                     /locus_tag="JYG31_RS19640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19640"
                     /product="nitrate reductase subunit alpha"
                     /protein_id="WP_213418095.1"
                     /transl_table=11
                     /translation="MKKKKMSPLFRRLNYFSPIEHQSNKHSQTTYEDRDWENVYRNRWQ
                     YDKVVRSTHGVNCTGSCSWNIYVKNGIVTWEGQNLNYPSTGPDMPDFEPRGCPRGASFS
                     WYIYSPLRVKYPYVRSVLINLWREALQAHQNPLEAWKSIVENPDKAKSYKQARGKGGFV
                     RAEWPEVLKLISASLLYTVITYGPDRNIGFSPIPAMSMISHASGSRFMSLIGGPMLSFY
                     DWYADLPPASPQIWGDQTDVPESSDWYNSGYIITWGSNVPLTRTPDAHFLAEARYKGAK
                     VISISPDFAESSKFADDWLSIRQGTDGALAMAMGHVILQEFYVNQKTERFIEYAKQYTD
                     FPFFVTLTKEDGVYTAGRFLHAKDIGRQTEHDQWKPAVWNERTSAFAIPQGTMGSRWDG
                     QKKWNLHMIDEETGESIEPRLSVLGIEDEIGTVRIPYFSNEGNRVLERDLPIKKLALDN
                     EDVYVTTVFDLILANYGVNRGIGEKSACSYDDPEPFTPAWQEQVTGIKKEMVINIAREF
                     AQNAIDTEGRSMIIVGAGINHWFNSDTIYRAVLNLVLLVGAQGVNGGGWAHYVGQEKLR
                     PAEGWQTIANAKDWEGAPKLQNGTSFFYFATDQWRYEDQPVSDLASPIAASSRYKHHAD
                     YNVLAARLGWLPSYPTFDKNGIDLYKEAEKAGAKTTKDVADYVARQLQEKKLKFAIEDP
                     DNEANFPRNLFVWRANLISSSGKGHEYFLKHLLGTTNGLMNDDSDSIRPEEIKWREQAP
                     EGKLDLLINLDFRMAGTALYSDIVLPAATWYEKHDLSSTDMHPFIHPFAPAISAPWESK
                     SDWDIFKALSKAVSDLAEEVDMKPVKEAVAAPLLHDTMQEMAQPLGKIKDWSKGECEAV
                     PGKTMPNIQVVERDYKQIFHKMTALGPNAALKPSGTKGMSWSIADEYESLKKRLGEVTS
                     DSVAKGCPNISEAKQAAEAVLTLSSTSNGKVAVKAWESLEDITNLKLKDLAEEREEECF
                     TFEQITAQPKTVITSPAFTGSEKGGRRYSPFTTNVEKLIPWRTLTGRQSYYVDHELMME
                     FGETMATFKPILQHRPFLSKRPDQEGKEIVLNYLTPHNKWSVHSMYFDSLPMLTLFRGG
                     PTVWMNKDDAADTDIKDNDWIECFNRNGVVVARAVLSHRIPKGMAFMHHAQDRHINVPG
                     TKLTNNRGGTHNSPTRIHVKPTQMIGGYAQLSYGFNYYGPTGNQRDLNVVIRKLKEVDW
                     LED"
     misc_feature    complement(3984..3999)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     gene            complement(4074..4550)
                     /locus_tag="JYG31_RS19645"
                     /old_locus_tag="JYG31_19645"
     CDS             complement(4074..4550)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391610.1"
                     /locus_tag="JYG31_RS19645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19645"
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_044159794.1"
                     /transl_table=11
                     /translation="MNQCDYLFFLKQLPMFNEVPLSIVETLLNNGTFIRGSCDQDLSFL
                     HSQSIYIVLKGSIRFIDSRLPEGSQTVALWEKGDVFPIDQKGGMFLSPFISVRTSEDIL
                     MLNIPFYILKKIMSYYPQLQMNFLVMLQQNVFFSYELFLRYMHRSQDENAEPDS"
     misc_feature    complement(4608..4623)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.4"
                     /tool="antismash"
     gene            4693..5412
                     /locus_tag="JYG31_RS19650"
                     /old_locus_tag="JYG31_19650"
     CDS             4693..5412
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391611.1"
                     /locus_tag="JYG31_RS19650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19650"
                     /product="YwiC-like family protein"
                     /protein_id="WP_213418096.1"
                     /transl_table=11
                     /translation="MKALIPKQHGAWAMLLIPFCLGMVKGGAVLWHIPLFLGWLFLYLA
                     VYPVTLALKKKQINPYQKWIFSYSFPACSFLLAAVIHKPSFIWLAFSLLPLFLIHVYYA
                     RQKNERALLNDFAGVLFFCTGGLASCWLGTETLDGWAWFIFTQSALFFIGSSFYVKSVI
                     REKNNRVFAYWSWGYHLILPLLSAALGGGWAVLAFIPSSLRAWFFHGKKWPVQTIGILE
                     IVNASFFFAIMCIWLTR"
     gene            complement(5445..6161)
                     /locus_tag="JYG31_RS19655"
                     /old_locus_tag="JYG31_19655"
     CDS             complement(5445..6161)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391612.1"
                     /locus_tag="JYG31_RS19655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19655"
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_101864901.1"
                     /transl_table=11
                     /translation="MNFLSVRTSDKDLISSDLHQLLESISTKRKIEKHTYLFREGMDAE
                     ELYLIQSGLIEIGKLTADGKDLTLRICQKNDIVGELTLFTEEPRYMLSAKVLEDGEVLV
                     INKSRLEKELIHNGALTFEFMKWMSTHLRKIQSKIRDLLLHGKKGALYSTLIRLSNSYG
                     VNRSDGILINIALTNQDLAKFCAAARESVNRMLGDLRKQGVISIEDSGKIVLHQRDYLR
                     CEIECENCPLEICNID"
     gene            complement(6272..7459)
                     /locus_tag="JYG31_RS19660"
                     /old_locus_tag="JYG31_19660"
     CDS             complement(6272..7459)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391613.2"
                     /locus_tag="JYG31_RS19660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19660"
                     /product="nitrate/nitrite transporter"
                     /protein_id="WP_024123209.1"
                     /transl_table=11
                     /translation="MINRQHFQLSLQSLSLVAGFMVWVLISSLISQITLDIHLSKGEIS
                     LVTAIPVILGSLLRIPLGYLTNRFGARIIFMISFILLLFPVFWISIADSLFDLIAGGFF
                     LGIGGAVFSIGVTSLPKYYPKEKHGVVNGIYGAGNIGTAITTFAAPVIAQAAGWKSTVQ
                     MYMALLALFALLHFFFGDRHEKKVKVSVKTQIKAVYRNHVLWFLSLFYFITFGAFVAFT
                     IYLPNFLVEQFDLSPADAGLRTAGFIAVSTLLRPAGGWLADKVSPLRILMFVFAGLTLS
                     GVILSFSPTIGIYAFGALTVAVCSGIGNGTVFKLVPFYFSKQAGIANGVVSAMGGLGGF
                     FPPLILASVFQATGQYAIGFMALSEVALASFVIVIWMYWQERMKTHTERSGHSVN"
     misc_feature    complement(7524..7539)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     misc_feature    7536..7551
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.43"
                     /tool="antismash"
     misc_feature    7542..7555
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.45"
                     /tool="antismash"
     misc_feature    7543..7556
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.87"
                     /tool="antismash"
     misc_feature    7544..7557
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.38"
                     /tool="antismash"
     gene            complement(7594..9264)
                     /gene="argS"
                     /locus_tag="JYG31_RS19665"
                     /old_locus_tag="JYG31_19665"
     CDS             complement(7594..9264)
                     /EC_number="6.1.1.19"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="argS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391614.1"
                     /locus_tag="JYG31_RS19665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19665"
                     /product="arginine--tRNA ligase"
                     /protein_id="WP_024123210.1"
                     /transl_table=11
                     /translation="MNIAEQMKDVLKEEIRAAVLKAGLAEESQIPNVILETPKDKTHGD
                     YSTNMAMQLARIAKKAPRQIAEEIVASFDKGKASIEKLDIAGPGFINFYMNNQYLTKLI
                     PSVLEAGDAYGETNIGNGERVQVEFVSANPTGDLHLGHARGAAVGDSLCNVLAKAGYDV
                     SREYYINDAGNQINNLALSVEVRYFEALGLEKPMPEDGYRGEDIISIGKRLAEEYGDRF
                     VNEEESERLAFFREYGLKYELEKLRVDLENFRVPFDVWYSETSLYQNGKIDKALDALRE
                     KGHVYEEDGATWFRSTTFGDDKDRVLIKKDGTYTYLLPDIAYHKDKLDRGFDKLVNVWG
                     ADHHGYIPRMKAAIEALGYEKGTLEVEIIQLVHLYKNGEKMKMSKRTGKAVTMRDLIDE
                     VGLDAVRYFFAMRSADTHMDFDLDLAVSTSNENPVYYAQYAHARICSMLRQGEEQGLKP
                     AADLDFSPIQSEKEYDLLKTIGGFPEAVAEAAEKRIPHRVTNYIYDLASALHSFYNAEK
                     VIDPENEEKSRARLALMKATQITLNNALQLIGVSAPEKM"
     gene            complement(9261..9689)
                     /locus_tag="JYG31_RS19670"
                     /old_locus_tag="JYG31_19670"
     CDS             complement(9261..9689)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391615.1"
                     /locus_tag="JYG31_RS19670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19670"
                     /product="DUF1934 domain-containing protein"
                     /protein_id="WP_024123211.1"
                     /transl_table=11
                     /translation="MKQETPITIHVKSVIEDDGNEEVIEFRTTGFYYVKQNKVYLSYYE
                     EHDLGKVKTIVKVSEGEVLVMRSGAVKMNQRFVTGASTIAKYKMSFGELELKTSTKSIQ
                     SDLGEEQGRISIAYDMHVGDEQQHLHNMTITYEGGTHA"
     misc_feature    9895..9910
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            10001..10132
                     /gene="sboA"
                     /locus_tag="JYG31_RS19675"
                     /old_locus_tag="JYG31_19675"
     CDS             10001..10132
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /gene="sboA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: Subtilosin_A"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391616.1"
                     /locus_tag="JYG31_RS19675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19675"
                     /product="subtilosin A"
                     /protein_id="WP_003222002.1"
                     /sec_met_domain="Subtilosin_A (E-value: 9.2e-29, bitscore:
                     97.4, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKAVIVENKGCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
     CDS_motif       10001..10030
                     /prepeptide="leader"
                     /note="peptide class: sactipeptide"
                     /note="predicted sequence: MKKAVIVENK"
                     /locus_tag="JYG31_RS19675_sactipeptide"
                     /aSTool="sactipeptides"
                     /tool="antismash"
     CDS_motif       10031..10132
                     /prepeptide="core"
                     /locus_tag="JYG31_RS19675_sactipeptide"
                     /peptide="sactipeptide"
                     /predicted_class
                     /score="33.00"
                     /molecular_weight="0.0"
                     /monoisotopic_mass="0.0"
                     /core_sequence="GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
                     /aSTool="sactipeptides"
                     /tool="antismash"
                     /leader_sequence="MKKAVIVENK"
                     /leader_location="[10000:10030](+)"
     misc_feature    complement(10171..10187)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 22.25"
                     /tool="antismash"
     gene            10267..11613
                     /gene="albA"
                     /locus_tag="JYG31_RS19680"
                     /old_locus_tag="JYG31_19680"
     CDS             10267..11613
                     /codon_start=1
                     /gene="albA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: subtilosin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Subtilosin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thurincin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SPASM"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391617.1"
                     /locus_tag="JYG31_RS19680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19680"
                     /product="subtilosin maturase AlbA"
                     /protein_id="WP_024123212.1"
                     /sec_met_domain="subtilosin (E-value: 0.0, bitscore:
                     1073.7, seeds: 6, tool: rule-based-clusters)"
                     /sec_met_domain="Subtilosin_rSAM_RRE (E-value: 2.8e-29,
                     bitscore: 99.3, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 4.4e-08, bitscore: 31.3,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Thurincin_rSAM_RRE (E-value: 4.4e-07,
                     bitscore: 27.8, seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="PTHR43273 (E-value: 2.3e-44, bitscore:
                     149.8, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 1.5e-06, bitscore: 25.8,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 2.3e-20, bitscore: 71.6,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 7.7e-10, bitscore:
                     37.0, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 9.8e-09, bitscore: 33.6,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFIEQMFPFINESVRVHQLPEGGVLEIDYLRDNVSISDFEYLDLN
                     KTAYELCMLMDGQRTAEQILEEQCAKYDESPEDHKDWYYDMLSMLLNKQVIQLENRARF
                     HKIATSGSNEFPMPLHATFELTHRCNLKCAHCYLESSPEALGTVSIDQFKKTADMLYDN
                     GVLTCEITGGEIFVHPNANEILEYVLKKFKKVAVLTNGTLMRKESLEMLKAYKQKIIVG
                     ISLDSVQSEVHDSFRGRKGSFAQTCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKV
                     RDLGAKAFSYNWVDDFGRGRDIVHPTKDAEQHRKFMEYEQNVIDEFKDLIPIIPYERKR
                     AANCGAGWKSIVISPFGEVRPCALFPKEFSLGNIFHDSYESIFDSPLVHKLWKAQAPRF
                     SEHCMKDKCPFSGYCGGCYLKGLNSNKYHRKNICSWAKNEQLEDVVQLI"
     aSDomain        10282..10560
                     /aSDomain="Subtilosin_rSAM_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing rSAM protein in a subtilosin
                     sactipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_JYG31_RS19680_Subtilosin_rSAM_RRE.1"
                     /evalue="1.90E-31"
                     /identifier="RREFam018.1"
                     /label="JYG31_RS19680"
                     /locus_tag="JYG31_RS19680"
                     /protein_end="98"
                     /protein_start="5"
                     /score="99.3"
                     /tool="antismash"
                     /translation="MFPFINESVRVHQLPEGGVLEIDYLRDNVSISDFEYLDLNKTAYE
                     LCMLMDGQRTAEQILEEQCAKYDESPEDHKDWYYDMLSMLLNKQVIQL"
     gene            11626..11787
                     /gene="albB"
                     /locus_tag="JYG31_RS19685"
                     /old_locus_tag="JYG31_19685"
     CDS             11626..11787
                     /codon_start=1
                     /gene="albB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391618.1"
                     /locus_tag="JYG31_RS19685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19685"
                     /product="antilisterial bacteriocin subtilosin biosynthesis
                     protein AlbB"
                     /protein_id="WP_213418097.1"
                     /transl_table=11
                     /translation="MSPAQRKILLYILSFIFVIGAVVYFVKSDYLFTLISIAIAIIFGM
                     RARKADSR"
     gene            11784..12503
                     /locus_tag="JYG31_RS19690"
                     /old_locus_tag="JYG31_19690"
     CDS             11784..12503
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391619.1"
                     /locus_tag="JYG31_RS19690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19690"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_024123214.1"
                     /transl_table=11
                     /translation="MSILDIHDVSVWYEQDNVILENVDLHLEKGAVYGLLGVNGAGKTT
                     LINTLTGVNRNFSGHFTLCGIEADAGMPQKTSDELKIRRYFAADYPLLFTEITAKDYVS
                     FVHSLYQKDFSEQQFNSLAEEFHFSQYTNRRISELSLGNRQKVVLMTGLLLGAPLFILD
                     EPLVGLDVESIEVFYQKMQEYCKAGGTILFSSHLLDVVQRFCDYAAILHHKQIRKVIPI
                     GEETDLRREFFEVIGHE"
     gene            12496..13809
                     /locus_tag="JYG31_RS19695"
                     /old_locus_tag="JYG31_19695"
     CDS             12496..13809
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391620.1"
                     /locus_tag="JYG31_RS19695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19695"
                     /product="hypothetical protein"
                     /protein_id="WP_024123215.1"
                     /transl_table=11
                     /translation="MNNLFPILSLLFKQLYGRQGKKDAIRITAGLAVLALFEIGLIRQA
                     GIDESTLGKTYITLALLFMNAYMVFLSVTSQWKESYMKLSCLLPISSRSFWLAQSVVLF
                     VDTCLRRTLFFFILPLFLFGNGTLSGAQTLYWLGRFSFFTVYSIIFGVLLSNLFVKKSR
                     TALLLHAAAFTCVCFSAALMPAVTIPLCAIHMLWAIVIDFPSFLQAPQQQSKMHSFMRT
                     SEFSFYKREWNRFISSKAMLLNYIVMGAFSGFFSFQMMNTGIADQHVVYIVISALLLIC
                     SPIALLYSLEKNDRMLLITLPIKRRTMLWAKYRFYSGLLAGGFILIAMIMGLISGRPIS
                     LLTFLQCMELLLAGAYIRLISDEKKPSFSWQTEQQLWSGFSKYRSYVFCIPLFLATLTG
                     TVISLAVIPIAGLIIVYLLQKQDGGFFDTSGKRERLGS"
     gene            13799..14962
                     /locus_tag="JYG31_RS19700"
                     /old_locus_tag="JYG31_19700"
     CDS             13799..14962
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391621.1"
                     /locus_tag="JYG31_RS19700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19700"
                     /product="insulinase family protein"
                     /protein_id="WP_081638377.1"
                     /sec_met_domain="Peptidase_M16_C (E-value: 7.5e-05,
                     bitscore: 20.8, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEVNLLTTDQFSTISIAASFLKPIESAAEPEEETIYFYGAAAHQK
                     QNIINTFGYAAGRRFMYSANLFFDRQLKTYGTRMIHPLYAEGFHTDAVLNTFVDMSSFP
                     ASLSASAAEKAKAELLLKMEKSFADPFSYSAARLAEEAFGNPMYGTAMFGRKDKLMAVH
                     PKRFLDASEFIADLFSQQKQLNILGNIQSCDVQGHAPQASAMSAVRKPVNQDIFETETR
                     STAGPSVMTLGFDCGDMKDVRDYLAIQLIDGLLGKYGHSALFRHFREKDLAVYHVITRY
                     DIMSNMLLVSVCTDQLHENEIPPRVLEAVSHFHADEPELKKAQQFLINEILLQFDSPEG
                     LLAYMGILRRFSCKKEDLLDSISAVTCRDLLQFIKNLTYIGAHVVRG"
     gene            14968..16245
                     /locus_tag="JYG31_RS19705"
                     /old_locus_tag="JYG31_19705"
     CDS             14968..16245
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391622.1"
                     /locus_tag="JYG31_RS19705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19705"
                     /product="pitrilysin family protein"
                     /protein_id="WP_213418098.1"
                     /sec_met_domain="Peptidase_M16 (E-value: 1.9e-12, bitscore:
                     45.4, seeds: 24, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_M16_C (E-value: 1.3e-06,
                     bitscore: 26.5, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKEAFFQQLDERTDIRYTDKGMKIFRLQFPRAHLRLCNVKTGFG
                     SRDIRLQSETGGRMLPYGTAHFLEHLLFWHNGRNLYSDFFEHGALLNAFTTYTDTNFMF
                     TSLPDRLRQTIPILLDALWNHSFDEQIIAQEKAVITSEIETALLNHQLHYHYQLIQMLS
                     PASPAAVFPAGRKKDIEALTICDLQEAYAAAYQPHRMTLFLIGGPEDAETLLPSQLRLE
                     KRSGSRSGRKIVPACPPLSPKEVKGDLELPEDTWTGIQVGALPGENGLLTLKLYWDIAS
                     RILFHMDSPFFQEIRQTYRLNIQSLSADVHLSEDGGFLILHSKGAHSSAYIDVASYYIT
                     QQKNRTEAWLEYGKDSLVNAIIYDSDYARKCFEWAAECEQYDCSFLGLYRTIQQMNFHD
                     FLALIEAMVSSNKAVIHVPQKEVMRQ"
     gene            16242..16943
                     /locus_tag="JYG31_RS19710"
                     /old_locus_tag="JYG31_19710"
     CDS             16242..16943
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391623.1"
                     /locus_tag="JYG31_RS19710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19710"
                     /product="bacteriocin biosynthesis protein AlbG"
                     /protein_id="WP_213418099.1"
                     /transl_table=11
                     /translation="MKKSTVFTLLLLLAAMAAYSFGWFHSGAEAAGQYVQLIKNDAARL
                     GLLACAASVLMLPAFLYLHYVTQSVKNMTAAFQKLTQSHQSCCDFQHRKLCSRYADDVK
                     LLRDSYKGIRQSYVMAAVLYQVIVFGCMFEIVKAVPFSFHTPPAVTMGLAVLLILYLLF
                     YMRKYLLQLFQHGNLFRKVLVGVLTGAGIWWMLSFTISELLFLIILAAIQQIGSFVYKR
                     ISYRSPASLDL"
     gene            complement(16950..>17416)
                     /locus_tag="JYG31_RS19715"
                     /old_locus_tag="JYG31_19715"
                     /pseudo=""
     CDS             complement(16950..>17416)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391624.1"
                     /locus_tag="JYG31_RS19715"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="JYG31_19715"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="ASREVPFQVPIQGFVVDPAEAVSAVTWSTDVMNGELCRRV"
     gene            complement(17510..18862)
                     /locus_tag="JYG31_RS19720"
                     /old_locus_tag="JYG31_19720"
     CDS             complement(17510..18862)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391625.1"
                     /locus_tag="JYG31_RS19720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19720"
                     /product="YncE family protein"
                     /protein_id="WP_198098140.1"
                     /transl_table=11
                     /translation="MRKNKLSFKEKAEAAQDCLCFCEEETSREAMFQAPIELPEGFVVD
                     PAEAVANVTWNADSLSCVSERCLIETGPEPDEIGVQFAVRLQGTVTLLVSVSPVRNQYG
                     QGEGAISVIHNVEIDQVVYYSDEPDDCPDVSDITIENLVVVPPFYGSPLSVTGTIVLAP
                     EPEPVYAFTANPDDQSVSVIDTNTHTVVTTISLPYQPTRVEKTPDNTSVFVLHYYDRVI
                     TVINQDTLSVTASILLDQPPESISFIPGHEFFYVVAGGAIYVIGIETLTVERSIPFEGF
                     DVAIDPNGLFAYLLGFGSVQRLDLNTGEVTGTIERDLIVNALETGWPERYLYVLEQEFF
                     FNYLTVIDLNTFTISSTQELEYEGEYGMFKSGSGVYLFDDFTHNLYSVSPSGAGVIGNL
                     PQSARDFAFTPNGEFLYATNFIEQSITVYNTDDYSVETVISLGVSPSDIAI"
     misc_feature    19042..19058
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 22.81"
                     /tool="antismash"
     gene            19091..20236
                     /locus_tag="JYG31_RS19725"
                     /old_locus_tag="JYG31_19725"
     CDS             19091..20236
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391626.1"
                     /locus_tag="JYG31_RS19725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19725"
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_024123222.1"
                     /transl_table=11
                     /translation="MTSVISSSSVGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEE
                     DQDLHLYYSLMEFRHNLMLEYLEPLENMRIEEQPRLSDLLLEIDKKQARLTGLLEYYFN
                     FFRGMYELDRREYILAIQFFKKAESKLTFVKDKIDKAEFFFKMSEVYYYMKQTYVSMDY
                     ARQAFEIYNEQTTYDIRMIQCQSLFGANFLDLKQYDKAIAHFQKAYTLAEAAQQPQLMG
                     RTLYNIALCKQNQHSLESAIEYVKKSIHVFEESKMLPSLPQAFFLITQIYFKLGKIDNA
                     CEYHKKGREYSIAAGDTVYLMEFEFLESLYLSGPDEEAIEKCFVFLESKMMYADIEDFA
                     LDVAKYYHQQENYKKASTYFLKVEEARQHIQGGVNLYEMEV"
     gene            20220..20339
                     /locus_tag="JYG31_RS19730"
                     /old_locus_tag="JYG31_19730"
     CDS             20220..20339
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003326489.1"
                     /locus_tag="JYG31_RS19730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19730"
                     /product="PhrC/PhrF family phosphatase-inhibitory
                     pheromone"
                     /protein_id="WP_024123223.1"
                     /transl_table=11
                     /translation="MKWKYKLLLACLAVSAVFVTVEVSNSSTEHFDVAQRAMI"
     gene            complement(20446..21318)
                     /gene="speB"
                     /locus_tag="JYG31_RS19735"
                     /old_locus_tag="JYG31_19735"
     CDS             complement(20446..21318)
                     /EC_number="3.5.3.11"
                     /GO_function="GO:0008783 - agmatinase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="speB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391629.1"
                     /locus_tag="JYG31_RS19735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JYG31_19735"
                     /product="agmatinase"
                     /protein_id="WP_095712788.1"
                     /transl_table=11
                     /translation="MRFDEAYSGKVFIASRPEWEEADAILYGMPMDWTVSYRPGSRFGP
                     GRIREVSIGLEEYSPYLDRDLADLNFFDAGDIPLPFGNPQKSLDMIEEYVDSILEKGKF
                     PMGMGGEHLVSWPVMKAMYKKFPDLAIIHFDAHTDLRVDYEGEPLSHSTPIRKAAELIG
                     PQNVYSFGIRSGMKEEFEWAKENGMHISKFEVLEPLKKVLPKLAGRPVYVTIDIDVLDP
                     AHAPGTGTVDAGGITSKELLASVHEIARSEVNVKGADLVEVAPIYDHSEQTANTASKII
                     REMLLGFVK"
ORIGIN
        1 gttttcggtc ctgatttcag ctcaagcttt ccttttttta atgtccagcc gcctttatat
       61 ttgtcttggt cctcccactg cttcggatag ccgattccag gttttgtttc tacattattg
      121 aagtacatat attccgctcc ggaacggttt gtccacgtgt ttttgcaggt cacgctgcat
      181 gtgtggcagc cgatgcattt atctaagttc atgaccattc cgatttgcgc tttaatcttc
      241 aagccaatcg acctccttca gcttgcggat gaccacattc agatcgcgct gattccccgt
      301 agggccatag tagttaaagc cgtagctgag ctgggcatag ccgccgatca tctgtgtcgg
      361 cttgacgtga attcttgtcg gactgttatg ggtgcctccg cggttattgg tcagttttgt
      421 gccgggcacg ttgatgtggc ggtcttgggc atggtgcata aacgccattc cttttggtat
      481 ccggtgagac agaaccgctc tggctacgac aacaccattt cggttaaagc attcaatcca
      541 atcgttgtct ttaatatccg tatctgccgc atcatcttta ttcatccata ctgtcggccc
      601 gccgcggaat agcgtaagca tcggcaggga atcaaaatac atgctgtgca cggaccattt
      661 attatgcggc gttaaataat tgagaacgat ttctttgccc tcttgatctg gccgtttgct
      721 taagaaaggg cgatgctgga ggatcggttt aaaggtcgcc atcgtttccc cgaactccat
      781 catcagctca tgatcgacat aataggattg tctgcctgtc agcgtacgcc aagggattaa
      841 tttttccaca tttgtcgtaa acggcgaata cctgcgcccg cctttttccg agcctgtaaa
      901 tgccggagat gtgatcaccg ttttgggctg agccgtgatt tgttcgaacg taaagcattc
      961 ttcctcccgc tcttcagcca gatctttcag cttcagattg gtgatatctt caagtgattc
     1021 ccatgctttt acggcaactt ttccattcga cgtggacgaa agggttaaaa cggcttctgc
     1081 cgcctgcttg gcctcgctta tattcggaca tccctttgcg acactgtctg aggtgacttc
     1141 tcccagcctt tttttgagtg attcgtattc atcggcaatc gaccagctca tgccttttgt
     1201 tccgcttggt tttaaggcgg cgtttgggcc gagcgcggtc attttatgaa agatttgttt
     1261 gtagtccctt tcaacgactt gaatgttcgg cattgttttc cccgggacgg cttcgcattc
     1321 gcctttgctc cagtccttga tttttccgag aggctgcgcc atttcctgca tcgtgtcatg
     1381 gaggagcggt gcggcgacag cttcttttac cggcttcata tcaacttctt ccgcgagatc
     1441 ggagacggct tttgataaag ccttgaaaat atcccagtcc gacttcgatt cccaaggagc
     1501 cgaaatagcc ggggcaaagg ggtggataaa cggatgcata tcagtgctgc tgagatcgtg
     1561 tttttcatac catgtcgccg ccggaagcac gatatcggaa tacaaggcgg taccggccat
     1621 tcggaaatcg agattgatta acaaatcgag ctttccttcc ggtgcctgct cgcgccactt
     1681 gatttcttca ggacggatac tgtcgctgtc gtcattcatc aagccgttcg tcgttcccaa
     1741 gagatgcttg agaaaatatt catgcccttt tcccgaactg gagatcagat tcgccctcca
     1801 cacgaacagg tttctcggaa aattcgcttc attgtcagga tcttcaatgg caaatttcag
     1861 ttttttctct tgaagctggc gggccacata gtcagcaacg tcttttgttg tcttcgctcc
     1921 cgctttttca gcttctttgt acaggtcaat tccgttttta tcaaatgtcg gataagacgg
     1981 aagccacccg agtcttgccg ccagcacatt gtagtcggcg tgatgcttgt agcgggagga
     2041 tgccgcgatc ggagatgcta agtcactgac cggctgatcc tcgtagcgcc actggtcagt
     2101 cgcaaaatag aaaaaggaag tgccgttttg cagctttggc gccccctccc aatccttagc
     2161 gttggcgatc gtctgccagc cttcagcagg gcggagtttt tcctgtccca cataatgagc
     2221 ccagcctccg ccgtttacgc cttgggcgcc taccagtaaa acaagattta acaccgcccg
     2281 gtagatcgtg tctgagttga accagtggtt gatccccgct cctacgataa tcatggaccg
     2341 gccctctgta tcgatcgcat tttgagcaaa ttccctagca atgttaatga ccatctcttt
     2401 tttgatccct gtcacctgct cctgccaggc tggcgtaaac ggctcaggat cgtcatacga
     2461 gcaagctgat ttttccccga tcccccgatt cacgccgtag ttagccaaaa tcaagtcaaa
     2521 tacagttgtg acatacacat cttcattgtc tagggccagt ttttttatag gcagatctcg
     2581 ctcaagcact ctgtttccct catttgagaa ataagggatt cgaaccgtgc cgatttcgtc
     2641 ctctatgccg agcaccgaaa gccggggttc aatcgattcg cctgtttctt catctatcat
     2701 gtgcaggttc catttctttt ggccgtccca gcgggagccc attgttcctt gcgggatcgc
     2761 gaatgcgctt gtacgttcat tccacaccgc aggcttccac tgatcatgct ccgtctgccg
     2821 cccgatgtcc tttgcatgaa gaaaacgccc ggccgtatac acgccatcct ctttagttag
     2881 agtgacaaag aacgggaaat ccgtatattg ctttgcatac tctatgaaac gctctgtctt
     2941 ctgattaacg taaaactcct gcaggatcac atgacccatc gccatcgcaa gcgcgccgtc
     3001 tgttccttgg cggatgctca gccagtcatc tgcaaattta gaggattccg cgaaatccgg
     3061 gctgattgag atgaccttag cgcctttata gcgggcttct gccaaaaaat gcgcatccgg
     3121 cgtcctcgtt aacggcacat tggagcccca tgtgatgata tagcctgagt tgtaccaatc
     3181 actgctttcc gggacgtctg tctggtcgcc ccagatttgc ggggatgccg ggggaagatc
     3241 cgcataccaa tcatagaaac tgagcatagg gccgccaatc aaagacataa accgcgagcc
     3301 tgacgcatgg ctgatcatgg acatagccgg aatcggagaa aacccgatat tccggtcagg
     3361 gccgtatgtt ataaccgtat acagcaaaga agcagaaatc agcttcagca cctccggcca
     3421 ttcagcgcgc acaaagccgc cctttccccg cgcctgttta taggacttcg ctttatccgg
     3481 attttcaacg atcgatttcc atgcttccaa agggttttga tgagcctgca atgcttcccg
     3541 ccacaaattg atcagcacac tgcgcacgta cggatatttc acacggagcg ggctgtaaat
     3601 ataccatgaa aaactggccc cccgcggaca gcctctcggt tcgaaatcag gcatatccgg
     3661 gcctgttgaa ggataattca aattttgccc ttcccacgtg acgatgccgt ttttcacgta
     3721 tatattccag ctgcaggacc ctgtgcagtt gacaccgtgg gtggagcgga cgaccttatc
     3781 gtactgccat ctgtttctgt atacattctc ccaatcacga tcttcatagg ttgtttggct
     3841 atgtttattt gattgatgct caataggaga gaaataattt aatctcctaa acaacggact
     3901 cattttcttt tttttcatga atatttcact ccttccgagt caggtattct aagttcacta
     3961 tatacagagg catgatgttt ccttgtgaac tatgtcacac actccccttt tttcacagaa
     4021 agttcacatg aggaagaagg aatggaaata ccgaacaaaa aacccggttc cttttaggaa
     4081 tcgggctcag cgttttcatc ctgagatcta tgcatgtatc gcaaaaacag ctcataagag
     4141 aaaaagacat tttgctggag catcaccaga aaattcattt gcagctgcgg ataataggac
     4201 atgattttct ttaatatgta aaaagggata ttgagcataa gaatgtcttc ggatgttcgg
     4261 actgaaatga atggagataa gaacatcccg cctttttgat caatgggaaa cacatcgcct
     4321 ttttcccaca gcgccacggt ttgcgaccct tcaggcagcc tgctgtcaat aaaacgaata
     4381 ctgcctttta atacaatata aatggattga gaatggagaa aagatagatc ctgatcacat
     4441 gaaccgcgga taaacgtccc gttattcagc aatgtttcga caatggatag aggcacttca
     4501 ttaaacatag gaagttgttt caaaaaaaat aaatagtcac actgattcat agggaagcct
     4561 cctgtgctaa ggataccttt agtgtagccc ggccgatcca tgacagcagt gatgtatttc
     4621 acagtgatat gatgaatgtc acttattggc ggtatttttg cttttataat gaaacaagaa
     4681 aggagtgttt cgatgaaggc gcttattcca aagcagcacg gcgcttgggc catgctgctg
     4741 attccgtttt gcctcggtat ggtgaaggga ggagcggttc tttggcatat ccccctattt
     4801 ttaggctggc tttttcttta tttagctgtt tatcctgtta cgctggcatt aaaaaagaaa
     4861 caaatcaacc cctatcaaaa gtggatattt tcttacagtt ttccagcctg cagctttttg
     4921 cttgccgctg tcatccataa gccgtctttt atctggcttg ccttttcttt gctgccgctg
     4981 tttctcattc atgtgtatta tgcccggcaa aaaaatgaac gagcgctgct gaatgatttt
     5041 gcgggcgtct tattcttttg tacaggcgga ctcgccagct gctggctggg gacggagact
     5101 cttgacggct gggcatggtt tattttcaca caatctgctt tgttttttat cggaagttca
     5161 ttttatgtga agtcggtgat ccgggagaag aataatagag tatttgctta ctggtcgtgg
     5221 ggctatcacc tgatcctccc cttactttcc gcagctctcg gaggtggatg ggccgttctc
     5281 gcctttatcc ccagcagttt gcgtgcatgg ttttttcatg gaaagaagtg gcctgttcaa
     5341 acgatcggca ttcttgaaat cgtcaatgcg agcttctttt tcgccatcat gtgtatttgg
     5401 ctaacaagat aaaaacaagg acatattgct gtccttgttt cctcttaatc aatattacaa
     5461 atctctaacg ggcaattttc gcactcaatc tcacaccgta aatagtcccg ctgatgcagt
     5521 acaatctttc cggaatcctc tattgaaata accccttgtt tgcggagatc ccccagcatg
     5581 cggttgacgc tctctcttgc agccgcgcaa aattttgcca aatcctggtt tgtcagggcg
     5641 atgttaatga gaatgccatc actccggttc accccatagc tgtttgacaa gcggatgagg
     5701 gtggagtaga gcgccccttt tttgccatga agaagaaggt ctctgatttt ggattgaatt
     5761 ttgcgcaaat gggtgctcat ccatttcata aattcaaatg tcaaagcacc gttgtggata
     5821 agttcctttt caagtctgct tttattgatg acgagcactt cgccatcctc cagcactttt
     5881 gcactgagca tatatctcgg ttcttctgtg aaaagtgtta attcaccgac aatatcattt
     5941 ttttgacaga ttctaagcgt caggtctttg ccgtcagccg tcagcttccc gatttcaatg
     6001 agtcctgact gaatcagata aagctcttct gcatccattc cttctcgaaa caggtatgtg
     6061 tgtttttcga tttttctttt tgtgctgatg gattccagca attgatgaag atcactggaa
     6121 ataagatcct tatcagatgt tcgaacagag agaaagttca tgagctcacc tcgaagaaaa
     6181 gtcacgttgt gtatctattg taacgtgaat tcatttgcgc ttttcagaaa aaacttacaa
     6241 tctcgtgaat tcccaaatcg ccggcggccc gtcagtttac gctgtgcccg cttctctctg
     6301 tgtgtgtttt cattctttcc tgccaataca tccatatgac aatgacaaag cttgctaatg
     6361 cgacttccga aagcgccata aatccgatgg cgtattgtcc cgtcgcctga aatacactcg
     6421 ccagaatcag cggagggaaa aagccgccta atccgcccat ggcagacaca actccgttgg
     6481 cgattcccgc ctgcttagaa aaatagaaag gaaccagttt aaaaaccgtc ccgtttccaa
     6541 tcccggagca caccgcaacc gttaacgccc caaaggcata aatgccgatc gtcggcgaaa
     6601 acgacaggat gacgcccgat aatgtcaggc cggcaaagac aaacatcagg atgcggagcg
     6661 gactcacttt atcagccagc cacccgcccg ccggcctgag cagcgtggat acagcaataa
     6721 aacctgccgt ccgcagcccg gcatccgcag gactcagatc aaactgctca acaagaaagt
     6781 ttggcaaata gattgtaaaa gcgacaaagg cgccaaacgt aataaaataa aagagactca
     6841 gaaaccacag cacatgattt cggtaaacgg ctttgatttg agtctttacg gacaccttca
     6901 ctttcttctc atgccggtca ccgaagaaaa agtgcagcaa agcaaataaa gccagcagag
     6961 ccatatacat ttgcaccgta cttttccacc ccgccgcttg agcaatcacg ggagctgcaa
     7021 atgtagtgat cgcggttccg atatttccgg caccgtaaat accgtttaca acgccatgtt
     7081 tttctttagg atagtacttc ggaagagatg taactccaat ggaaaaaacg gctcccccaa
     7141 tccctaagaa aaaaccgccc gcgattaaat caaacaggga atctgcaata ctgatccaaa
     7201 atacagggaa taaaagcaat ataaagctga tcataaaaat gatccgcgcg ccaaacctgt
     7261 tggtcaaata ccctagcgga atgcggagaa gcgatcctaa tataacgggt atggccgtta
     7321 ccaaggaaat ttcgcctttg ctcaaatgaa tatctaatgt gatttgggaa ataagtgacg
     7381 aaatcagcac ccaaaccata aaccctgcca ccaagcttaa tgactgcagc gataattgaa
     7441 agtgttgacg gttaatcatt cggcttgccc ctttcactga ttcaataatc agccttatca
     7501 tacaaaaact cggcgaggaa gattgtgaac tatatcacac gattttttaa caaaaaaagg
     7561 acaaagccct ctggctttgt cctttcagct tgattacatt ttttcaggag ccgatacgcc
     7621 gatcaattga agcgcgttgt tcagcgtgat ttgcgttgct ttcattaaag ctaagcgcgc
     7681 acggcttttc tcttcgtttt cagggtcgat gactttctcc gcattgtaga agctgtgcag
     7741 agctgaagcc aagtcataaa tataattcgt cacacggtgc ggaattcgtt tttccgccgc
     7801 ttccgccact gcctctggga aaccgccgat tgttttcagc agatcatatt ctttttctga
     7861 ttgaataggg ctgaaatcaa ggtctgccgc cggcttaagt ccttgctctt ccccttggcg
     7921 aagcatgctg caaatgcgcg catgtgcgta ttgcgcataa tacacagggt tttcgttaga
     7981 tgttgataca gcaagatcca agtcaaaatc catatgcgta tcagcgctgc gcatcgcgaa
     8041 gaaataacgc acggcatcaa ggccgacttc gtcaatcagg tcgcgcatcg ttaccgcttt
     8101 ccccgtccgt ttgctcattt tcattttctc gccgtttttg taaaggtgca cgagctgaat
     8161 gatttctact tccaacgttc ctttttcata gccaagcgct tcgattgccg ctttcatacg
     8221 cgggatgtag ccgtggtggt cggcgcccca tacgttgacc agcttatcaa agccgcggtc
     8281 aagcttgtcc ttatggtagg cgatatcagg aagcaggtat gtgtatgtgc cgtccttttt
     8341 gatcagtacg cggtctttgt catcaccgaa cgttgtggaa cggaaccaag tcgcaccgtc
     8401 ttcctcatag acatggcctt tctcacgcag cgcgtccagc gccttatcga tttttccgtt
     8461 ttggtacagt gatgtttcag aataccacac gtcaaacggc acgcggaagt tttccaagtc
     8521 tacgcgaagc ttctccagct cgtatttcaa accgtactca cggaaaaatg cgaggcgttc
     8581 gctttcttct tcgttgacga aacggtcgcc gtattcctca gcgagacgct ttccgattga
     8641 aatgatatcc tcaccgcggt agccgtcctc cggcatcggt ttttccagtc caagcgcttc
     8701 aaagtaacgg acttctacag aaagcgccag gttgttaatc tggtttccgg catcgttaat
     8761 gtagtattcg cggctcacat cgtaacccgc ttttgcaagc acattgcaca aggaatcgcc
     8821 gacagccgca ccgcgcgcgt ggcctaagtg aaggtccccc gtcggatttg cagatacgaa
     8881 ttccacttga acgcgttcgc cgtttccgat gtttgtttct ccatacgcgt ctcccgcttc
     8941 caatacagac gggatcagct tcgttaaata ttgattgttc atatagaaat tgataaaacc
     9001 aggaccggcg atatcaagct tttcaataga agcctttcct ttatcaaaag aagcgacaat
     9061 ttcctcagcg atttggcgcg gcgctttttt cgcgattctc gcgagctgca tcgccatatt
     9121 tgttgaataa tcgccgtgtg ttttatcctt cggtgtttct aaaatgacat ttggaatctg
     9181 gctttcttca gccagtcccg ctttcagaac ggctgctctg atttcttctt tcagcacgtc
     9241 cttcatctgt tccgcaatgt tcatgcgtga gttcctccct catacgtgat cgtcatatta
     9301 tgtaaatgct gctgctcgtc tccgacatgc atatcatatg cgatactgat acggccttgt
     9361 tcttcgccga gatcagattg aatcgatttt gtgctcgttt tcagctcaag ctcaccaaat
     9421 gacattttat atttggcgat tgtggaagct ccagttacaa aacgctggtt cattttgaca
     9481 gcgcctgatc tcatgacaag cacttcgcct tcgcttactt ttacaatcgt tttcactttg
     9541 cctaagtcgt gttcttcata ataagataga tacactttat tctgtttcac atagtaaaat
     9601 ccggtggtgc ggaattcgat cacttcttcg tttccgtcat cctcaatcac agatttcaca
     9661 tggattgtta tcggtgtctc ttgcttcatt ctgacacgtc cttttcatct ttccactttg
     9721 acttgaatag tataatcatt cggtttgtaa acttcaactg cttctatctt accatcattg
     9781 ctcatcagat ttgaagataa acctcagaaa aagcaattcc tttatagaag agaaaatcat
     9841 accactgatt acctttagga aatattacat ttttccgaat tctaccgttt acaattcaca
     9901 ttttcttcaa aatatatgta ttgaaatggt aatttgatag ttttaatata aaagtacatc
     9961 aaagatctgc tagaaaaaca aaaaaaggga ggattcaatt atgaaaaaag ctgtcattgt
    10021 agaaaacaaa ggttgcgcga catgttcaat cggagccgct tgtttagtgg acggacctat
    10081 ccctgatttt gaaattgccg gtgcaacagg tctattcggt ctctggggat aaggttctcc
    10141 ggaaaagggc atagtaaatt actatgccct ttttaactaa aatacaaatt ttaactaaaa
    10201 tctacttctt ggttatacat acacaatgat ttttatattt gaatcatatg tataggggat
    10261 ggtattttgt ttatagagca gatgtttcca tttattaatg aaagtgtaag agtccaccag
    10321 ttgcctgagg gcggtgtgct ggaaattgac tacttgcgtg ataacgtctc gatctctgat
    10381 tttgaatact tagatctcaa caaaacggct tacgagcttt gtatgctgat ggacggtcaa
    10441 agaacggctg aacaaatttt agaagagcaa tgcgcaaagt atgatgagtc gccggaagac
    10501 cataaggact ggtactatga catgctcagc atgcttttga acaagcaggt cattcagctt
    10561 gaaaaccggg cccgctttca caaaatcgcc accagcggaa gcaatgaatt tccgatgccg
    10621 ctgcatgcca cctttgagct gacgcaccgc tgcaatttaa agtgcgccca ctgctattta
    10681 gaaagctcgc cggaagcgct tggcactgta tccattgatc aatttaagaa aacggctgat
    10741 atgctgtatg ataacggtgt tttaacgtgt gaaatcacag gcggcgaaat tttcgtccat
    10801 ccaaacgcca atgagattct tgaatatgtg ttaaagaaat tcaaaaaagt cgccgtctta
    10861 accaacggaa cgctgatgcg aaaagaaagc ctggagatgt taaaagccta taagcaaaaa
    10921 atcatcgtcg gcatatcact ggatagcgta cagtccgaag tgcatgattc atttagaggg
    10981 agaaaaggct cttttgccca aacctgtaaa acgataaaat tgttgagtga tcatggtatt
    11041 tttgtcagag tcgccatgtc ggttttcgaa aaaaacatgt gggaaatcca cgatatggcc
    11101 caaaaggtgc gcgatctcgg ggcgaaagcg ttctcttaca attgggttga tgatttcgga
    11161 agaggcagag atattgtcca tccgacgaaa gatgccgaac agcaccgcaa atttatggag
    11221 tacgagcaaa atgtcattga tgaatttaaa gatttgatcc cgatcattcc gtatgaaaga
    11281 aaacgtgcgg cgaactgcgg cgccggctgg aaatcaattg tgatcagccc gttcggagaa
    11341 gtacgaccgt gcgcactctt cccgaaggaa ttttcattgg gaaatatatt tcatgattca
    11401 tatgaaagca tctttgactc ccctcttgtc cataaattgt ggaaagcgca ggcgccgcgg
    11461 ttcagcgagc attgcatgaa agacaaatgc ccgttcagcg gctattgcgg aggctgttac
    11521 ttaaaagggc tgaactccaa caaataccac agaaaaaaca tctgctcctg ggcgaaaaat
    11581 gaacaattag aagatgtggt ccagcttatt taggagggaa acaatttgtc accagcacaa
    11641 agaaaaattc tactgtatat cctttcattt atttttgtca tcggcgcggt cgtctacttc
    11701 gtaaaaagcg attatttgtt tacgctgatt tccatcgcca tcgccattat tttcggtatg
    11761 cgcgcgcgga aggctgactc gcgatgagca ttttggatat acacgatgta tccgtgtggt
    11821 acgagcaaga caacgtcatc ttagaaaacg tagatttaca tttagaaaaa ggcgccgttt
    11881 acggactgct tggggtaaac ggcgccggaa aaacaacgct gatcaatacg ctgacagggg
    11941 taaaccgcaa tttcagcgga cactttacgc tgtgcggcat tgaagcggac gccggcatgc
    12001 cgcagaaaac atcagatgag ctgaagatcc gccgctactt tgctgccgat tatccgcttc
    12061 tatttacaga aattacggcg aaggactatg tttctttcgt tcattctttg tatcagaaag
    12121 atttctcaga gcagcaattt aacagtttgg cagaggaatt tcacttttcc caatacacaa
    12181 accggagaat ttccgagctg tccctcggca acaggcaaaa ggttgtgttg atgaccgggc
    12241 tattgctcgg ggctcccctg tttattttgg atgagccgct tgtcggcttg gatgtggagt
    12301 caatagaagt cttttatcag aaaatgcagg agtattgcaa ggcaggcgga acgattttgt
    12361 tttcttccca tttgctcgat gtggtgcaga gattttgtga ttatgcggcc atccttcatc
    12421 acaaacagat ccggaaggtc attccgattg gagaggagac tgatcttcgg cgtgaatttt
    12481 ttgaggtgat cggccatgaa taacctgttt ccgattctgt cattgctgtt caaacagctt
    12541 tacggccgcc aaggaaaaaa agacgccatt cgcatcaccg cagggcttgc cgttctggcc
    12601 ttgtttgaaa tcgggctgat ccgccaggca ggcattgatg aatcaacctt aggaaaaaca
    12661 tacatcacac tcgcgcttct gtttatgaat gcgtacatgg tgtttctctc tgttacatcc
    12721 caatggaagg aatcttacat gaagctgagc tgtctattgc cgatctcttc ccggagcttc
    12781 tggctcgctc agagtgtggt tttgtttgtt gatacttgct tgagaaggac gctgtttttt
    12841 ttcatattac cgctgttctt attcggaaac ggaacgctgt caggagcaca aaccctgtat
    12901 tggctcggca gattttcgtt ttttactgtc tattcgatta ttttcggcgt tttgttaagc
    12961 aatcttttcg tcaaaaaaag cagaacggca ctcctgctgc atgcggcggc attcacctgc
    13021 gtgtgcttca gtgcggcttt gatgcctgcc gtgacgatcc cgctatgtgc gattcatatg
    13081 ctctgggcta ttgtgattga cttcccttca tttttgcagg cgcctcagca gcagagcaaa
    13141 atgcattctt ttatgcggac atcagaattt tcgttttata aaagagaatg gaaccggttt
    13201 atttcctcca aagcgatgct gttaaattat atcgtgatgg gggcattcag cggcttcttt
    13261 tcgtttcaga tgatgaacac cggcattgcc gatcagcatg tcgtctatat cgtaattagc
    13321 gctctcctgc tgatctgctc accgattgct ttattgtatt ctcttgaaaa aaatgaccgg
    13381 atgctgctga tcacgcttcc gattaaacgc agaacgatgc tttgggcgaa atatcgcttt
    13441 tattccggtt tattggccgg cgggtttatt ttgattgcca tgattatggg cttgatcagc
    13501 ggccggccga tttcactttt gacttttttg cagtgtatgg aattgctgct ggcaggggct
    13561 tatattcggt taatttcaga tgaaaaaaag ccgtccttca gctggcagac ggagcagcag
    13621 ctctggagcg gtttctctaa ataccgttcc tatgtatttt gtatccctct ttttttagct
    13681 actttaacag gcactgttat ttccttggca gtcattccga ttgccgggct gatcattgtc
    13741 tatttactgc aaaaacagga tggtggtttc tttgatacaa gcggaaaaag agaacggctt
    13801 ggaagttaac ctgctgacga ctgatcaatt ttcaaccatc tcgatcgccg cgagctttct
    13861 gaagccgatc gaaagcgcgg cggaacctga agaagaaacg atctattttt atggcgcggc
    13921 tgcacaccag aagcaaaaca tcatcaacac tttcggctat gccgccggcc gccgcttcat
    13981 gtattcagca aatctctttt ttgaccggca gctgaaaacg tacgggaccc gcatgattca
    14041 tccgttatat gctgagggat ttcatacaga tgccgttctg aacacctttg ttgacatgtc
    14101 ctcctttccg gcttctcttt ctgccagcgc agcggaaaag gcaaaagccg aactgctgct
    14161 gaaaatggaa aagtcattcg ctgacccgtt ctcctattca gcagcccgtc tggcggagga
    14221 agcctttgga aacccgatgt acggcactgc catgtttggc cggaaagaca agcttatggc
    14281 tgtgcatcct aaacgttttc tggatgcgtc ggaatttatc gctgatttat tttctcagca
    14341 gaaacagctg aacatattag gaaacataca gtcctgtgat gttcagggac atgccccaca
    14401 ggcgtctgcc atgtctgctg taagaaaacc cgtgaatcag gatattttcg aaacagagac
    14461 acgcagcaca gcaggtccgt ccgttatgac acttggcttt gactgcggag acatgaaaga
    14521 tgtccgcgat tatttagcca tccagctgat agacggattg ctcggcaagt acggccattc
    14581 cgctttattt cggcattttc gcgaaaaaga tctggccgtt tatcacgtga tcacccgcta
    14641 tgacatcatg agcaatatgc tgcttgtttc cgtgtgcacc gaccagcttc atgaaaacga
    14701 gattcctcca agagttttgg aagctgtatc gcatttccat gcggatgaac cggaattaaa
    14761 aaaggcacaa caatttttaa tcaatgaaat cctgctgcaa tttgattctc ctgaaggact
    14821 cttagcctat atgggaatcc tgcgccgttt ctcttgcaaa aaagaagacc tgctggacag
    14881 catctcagcg gtcacgtgcc gcgatttgct gcaattcatc aaaaatctta catatattgg
    14941 agctcatgta gtaaggggat gagagagatg gaaaaggaag ccttttttca acagctggat
    15001 gaacgaaccg atatcagata cacagacaag ggaatgaaaa tattcaggtt acaatttccc
    15061 cgcgcccatt taagattgtg caatgtcaaa accggctttg gaagccggga tatccgctta
    15121 cagtcagaaa ccggcggccg gatgctgcca tatggcacag cgcacttttt agaacatctc
    15181 ctcttttggc ataacggccg gaacctgtat agtgattttt ttgaacacgg cgctctgttg
    15241 aatgcgttca ccacttacac tgatacaaat ttcatgttta cctcattgcc ggatcggctc
    15301 agacaaacca ttccaatcct gcttgacgct ttatggaacc actcgtttga cgagcagatc
    15361 attgctcagg aaaaagccgt gattacaagt gaaatagaaa cggcgcttct caaccaccag
    15421 cttcattacc attatcagct gatccagatg ctgtctcctg cctcaccagc cgcagtcttc
    15481 cccgcaggca gaaaaaaaga cattgaggct ctgacgatct gtgatctgca ggaagcctat
    15541 gctgccgcct accagcccca tcggatgacg ctgtttctga ttggcggccc agaagatgcg
    15601 gaaactcttt tgccttctca gctcaggctg gaaaagcgct ctggcagccg ctctggcaga
    15661 aagatcgtcc ctgcgtgccc tcccctctca ccaaaagagg taaagggtga tctggagctg
    15721 ccggaagaca cttggaccgg aatacaagtc ggggcgctcc caggagaaaa cggacttttg
    15781 accttgaagc tgtactggga cattgcctct cgcatcctgt ttcacatgga ttcgccgttt
    15841 tttcaagaga tccggcaaac gtaccgcttg aatatccaaa gcctatcagc ggatgttcac
    15901 ttatcagagg atggcggctt tttgatcctt cacagtaaag gggcgcattc ttccgcatac
    15961 atcgatgtgg catcttacta tatcacgcag cagaaaaacc gtaccgaagc ctggcttgag
    16021 tacggcaagg attctttagt caacgcgatc atctatgaca gtgattacgc gcgtaaatgc
    16081 tttgaatggg cggcggagtg tgaacagtac gattgctcgt ttctcggcct ttaccgcacc
    16141 attcagcaga tgaattttca tgatttcttg gctctcattg aagcaatggt ttcttctaac
    16201 aaagcagtca ttcatgtacc ccagaaagaa gtgatgagac aatgaaaaaa agcactgtct
    16261 ttactttatt attgctgctt gcggccatgg cggcttattc cttcggctgg tttcactcag
    16321 gtgctgaagc cgcgggccaa tatgtacagc tgatcaaaaa tgacgctgcc cgacttgggc
    16381 tgttggcttg tgccgcttcc gtactgatgc tgcccgcctt tctctatctt cactacgtga
    16441 cgcaatctgt caaaaacatg acagcagctt ttcagaaact cacacagtct catcaaagct
    16501 gctgtgactt tcagcatcgc aagctgtgca gccgttatgc cgacgatgta aaattgctgc
    16561 gcgacagcta taagggcatc cgccaatcat atgtgatggc tgctgtattg tatcaggtca
    16621 tcgttttcgg ctgcatgttt gaaatcgtca aggccgttcc cttcagcttt catacgcccc
    16681 ctgccgttac aatggggctt gcggtgttac tgattttgta tttattgttt tatatgcgaa
    16741 agtacttgct gcagcttttt cagcacggaa acctgttcag aaaagtgctg gtgggagtgc
    16801 tgacaggagc cgggatttgg tggatgctca gttttactat aagtgaactg ctgtttctga
    16861 tcatcttagc tgccatccag cagatcggat cttttgttta taaacgaatc tcttaccgca
    16921 gccctgcttc tctcgatcta taaaaacatc tacatcgcaa tcgcggacgg accggcgcca
    16981 accggaatga ccgtcacggc tgaatcatca ctcgtgcggt acacggtcac tgcattgtcg
    17041 ccgggctgtg ccgcgtaaat gtattgattg tcgggcgtta ctgtaatgtc atagacatta
    17101 ggaagagaca cgtttgcgat gatcctttgt gcggcagtat taacgacata tacattgtta
    17161 atgagaaacg gttcactata aggctctaga aaataagccc tagtactatc cagagaaaat
    17221 acaattgtcc tcaaagtttc aaatagacgg tcaaagtcac ctcccctgct caagggtgac
    17281 aaactgatct ctgttacacc cttcggcaca gttcaccatt catcacgtct gtactccatg
    17341 taaccgcaga gactgcttcc gcaggatcaa cgacaaaccc ttgaatcggc acttgaaatg
    17401 gcacttcacg gctggcatct tcatcacaaa aacagaaaca attttcctgt aaaacagaaa
    17461 aatcattctt tttcatgatt tcacatccct tttagaaatg catagttgat taaatcgcaa
    17521 tgtcactcgg tgaaacgcca agcgatatga cagtttccac agaataatca tccgtgttgt
    17581 aaacagtgat gctttgctct ataaaatttg ttgcatacag aaattcgcca tttggggtaa
    17641 acgcaaagtc tcttgccgat tgcggaagat ttcctattac gcctgcacca cttggactga
    17701 cggaatatag attgtgagtg aaatcatcaa acaaatacac ccctgatccg ctcttgaaca
    17761 ttccatattc accctcatac tccagctctt gggtgctgct gatcgtaaat gtatttaaat
    17821 caatcaccgt caaataattg aaaaagaatt cttgttctaa tacataaagg taacgttcag
    17881 gccaacccgt ttctaaggca tttacgataa ggtcccgttc aatagttcct gtcacttcac
    17941 ctgtgtttaa gtccaatctt tgcactgaac cgaaaccaag cagatacgcg aacaatccat
    18001 ttggatcaat tgcaacatca aaaccttcaa aaggaataga cctttcaaca gttaaggttt
    18061 caattcctat cacgtatatg gcaccaccgg cgacaacata aaaaaattcg tgtccaggta
    18121 taaaagatat ggattccggc ggctgatcca gcaaaataga agctgtgaca ctcaatgtgt
    18181 cttgattgat gacagtaatg acacgatcat agtaatgcaa aacaaacaca gaggtgttat
    18241 ctggcgtctt ttctaccctt gtaggttggt atggaagaga aatcgtcgtc acaacagtat
    18301 gagtgtttgt atcaatcaca gaaaccgact gatcatctgg attcgctgta aacgcataaa
    18361 caggctccgg ctccggggcg agcacaatcg ttcccgtcac cgataaagga ctgccgtaaa
    18421 atggcgggac gacaacaagg ttctcgatcg taatgtcact gacatccgga caatcatccg
    18481 gttcatcaga ataatagaca acttgatcga tctctacatt gtgaatgaca gaaatagccc
    18541 cctccccttg tccatattgg tttcgcacag gtgagacact gacaagaagc gtaacggttc
    18601 cctgcaggcg gactgcaaat tgaacaccga tttcatcagg ctcaggcccc gtctcaatta
    18661 agcaacgttc actgacacaa gaaaggctgt ctgcattcca cgtgacgttt gcaacagctt
    18721 cagccggatc aacgacaaac ccctcaggca attcaatcgg cgcttgaaac atcgcttcac
    18781 ggctggtttc ttcctcacaa aaacataaac aatcttgtgc agcttctgct ttttctttaa
    18841 aggaaagctt gtttttcctc atttcatcac ttccttttca gacatacact gctcacgcag
    18901 atacaacaga atatggaagg tgaatgtgtt tgcttgtaca aacagccgag ggtaaaagct
    18961 gtttttgaag aaattccggc caccatgcgg tgtaacgaaa gaaaagattg cggttttttt
    19021 gcgcttctca caagaatgat gatgataaaa tatgacatat atagaataat gaagggaggg
    19081 attttcaaac gtgacaagtg tcatatcttc ttcttccgtt ggagaaaaga ttaatgaatg
    19141 gtatatgtac atacgccgat tcagcatacc ggatgcggaa tatttacgaa gagaaatcaa
    19201 acaagagctg gatcaaatgg aagaagacca ggatcttcat ttgtattatt cactgatgga
    19261 attccggcac aatctgatgc ttgagtatct tgaaccgtta gaaaacatgc ggatagagga
    19321 acagccgcga ctgtccgatt tgctgcttga aatcgataaa aaacaggctc gtttaaccgg
    19381 actgcttgaa tactacttta atttcttcag aggtatgtac gaactggacc gccgagaata
    19441 tatattagcc attcaatttt ttaaaaaagc agagagcaaa ctgacttttg ttaaagataa
    19501 aattgataaa gctgagttct ttttcaaaat gtcagaagtt tattactata tgaagcaaac
    19561 ttatgtgtcc atggactatg cacgtcaagc ctttgaaata tacaatgaac aaactacata
    19621 tgatatcaga atgattcaat gtcaatccct gtttggagct aactttttgg atctgaaaca
    19681 gtatgataaa gcaattgctc atttccaaaa agcttataca cttgctgagg cggcacagca
    19741 gccgcagcta atgggaagga cgctttataa tattgcactt tgcaagcaaa accaacacag
    19801 tcttgaatca gcgatagagt atgtaaaaaa atctattcat gtatttgaag aatcgaaaat
    19861 gcttccttca ctgccacaag cttttttcct aattacacaa atttatttta aattaggtaa
    19921 gattgataac gcttgtgaat atcataaaaa aggaagagaa tattcaatag ctgccggaga
    19981 cactgtgtac ttaatggagt ttgaattttt ggaatcttta tacttgtctg gacctgatga
    20041 agaggcaata gaaaaatgtt ttgttttcct tgaaagtaaa atgatgtatg ctgatattga
    20101 agactttgca ctcgatgttg ctaagtatta tcatcagcag gaaaattaca aaaaagcatc
    20161 tacttatttt ttaaaggttg aagaagcaag acagcacatt caaggggggg tgaatttata
    20221 tgaaatggaa gtataaatta ttactcgctt gtttagcggt gagtgctgta tttgtcacag
    20281 tagaagtatc aaattcatct acagaacatt ttgatgttgc acaacgtgct atgatctaag
    20341 accgccttta tcggcggttt ttttcgtccc ctctttccat cttgaataag atgtttgcaa
    20401 agcatgggaa caaaaaacat ccagacaaga gtctggatgt ttctcttatt ttacaaaccc
    20461 aagcagcatt tcacgaatga ttttgctggc tgtgtttgcc gtttgctctg agtggtcgta
    20521 aatcggagct acttctacta aatcagcgcc cttcacgttt acttctgagc gtgcgatttc
    20581 atggacggat gcaagcagtt ctttagacgt gatgccgcct gcgtcaaccg ttcctgttcc
    20641 cggtgcgtga gcagggtcta atacgtcaat gtcgattgtg acatataccg gacggcctgc
    20701 cagcttcgga agaacctttt tcagcggctc aagcacttca aacttagaga tgtgcatgcc
    20761 gttttccttt gcccattcga actcttcttt catgccggaa cgaattccga atgaatacac
    20821 attttgcggt ccgatcaatt ctgcagcttt gcggatcggc gttgagtgag aaagcggttc
    20881 tccctcatag tcgacgcgaa gatccgtatg tgcatcaaaa tgaataatcg ccaggtcagg
    20941 aaatttctta tacattgctt tcatgacagg ccatgacacg agatgctccc cgcccatacc
    21001 catcgggaat tttccttttt ctaaaatgct gtccacatat tcttcaatca tatcaagact
    21061 tttttgcggg tttccgaatg gcagcggaat gtcgccggcg tcaaagaagt tcaggtccgc
    21121 cagatcacgg tccagatacg ggctgtattc ttcaaggccg attgacacct cgcggattct
    21181 tcccgggccg aagcgtgatc ccggccgata gctcaccgtc cagtccatcg gcatgccgta
    21241 aaggatggcg tccgcctctt cccactctgg acggctcgcg ataaatactt ttcctgaata
    21301 agcttcatca aatctcatct ataaaaccct cgctttcctt taaaaaagcg caagattcgc
    21361 tcctgcgcca tacattcttt atttaatcag gtcactcaca aatttcggca gaacaaacgc
    21421 cgctttatga atatcttttg tgtaatattt cgtgtcaata tcaaagaaac ggctgtcttc
    21481 cactgcaagc ggatcatatt ttttcgaacc gattgtaaat gtccacaaac cgcttgggta
    21541 tgtcggaatg ttcgccgtat acagcttcgt aatcgggaag atttccttca cgtcacgctg
    21601 tacatttgtg atc
//
