LOCUS       NZ_BORO01000025        10454 bp    DNA     linear   CON 15-SEP-2024
DEFINITION  Oceanobacillus sp. J11TS1 sequence25, whole genome shotgun sequence.
ACCESSION   NZ_BORO01000025
VERSION     NZ_BORO01000025.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Oceanobacillus sp. J11TS1
  ORGANISM  Oceanobacillus sp. J11TS1
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Oceanobacillus.
REFERENCE   1
  AUTHORS   Okamoto,M., Kumagai,M., Kanamori,H. and Takamatsu,D.
  TITLE     Antimicrobial resistance genes in bacteria isolated from Japanese
            honey, and their potential for conferring macrolide and lincosamide
            resistance in the American foulbrood pathogen Paenibacillus larvae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 58087)
  AUTHORS   Okamoto,M., Kumagai,M., Kanamori,H. and Takamatsu,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2021) Contact:Daisuke Takamatsu National
            Agriculture and Food Research Organization; 2-1-2 Kannondai,
            Tsukuba, Ibaraki 305-8602, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.2
            Genome Coverage                   :: 1141x
            Sequencing Technology :: Illumina MiSeq; Illumina NovaSeq 6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018333215.1-RS_2024_09_15
            Annotation Date                   :: 09/15/2024 08:23:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,037
            CDSs (total)                      :: 3,937
            Genes (coding)                    :: 3,848
            CDSs (with protein)               :: 3,848
            Genes (RNA)                       :: 100
            rRNAs                             :: 9, 4, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8 (5S)
            partial rRNAs                     :: 1, 4, 7 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 32 of 89
            Pseudo Genes (incomplete)         :: 55 of 89
            Pseudo Genes (internal stop)      :: 14 of 89
            Pseudo Genes (multiple problems)  :: 12 of 89
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:11:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10454
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BORO01000025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10454
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RRE-containing"
                     /region_number="1"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /tool="antismash"
     cand_cluster    1..10454
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /kind="single"
                     /product="RRE-containing"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10454
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[196:454](-)"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(197..454)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
     misc_feature    64..82
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.4"
                     /tool="antismash"
     gene            complement(197..454)
                     /locus_tag="KI343_RS15735"
                     /old_locus_tag="J11TS1_31770"
     CDS             complement(197..454)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: PqqD_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017239.5"
                     /locus_tag="KI343_RS15735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J11TS1_31770"
                     /product="PqqD family protein"
                     /protein_id="WP_212927381.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 2.2e-13,
                     bitscore: 42.1, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PqqD_RRE (E-value: 5e-09, bitscore: 27.8,
                     seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 1.3e-16, bitscore: 52.6,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIKINKHIIFHEEKNESMLINFVDNYMIGLDQVSTLIWKELMKD
                     RDEIEIINYLAEKYQEDKKLLYKDVKEFLNDLEQAGVIKK"
     aSDomain        complement(203..436)
                     /aSDomain="PqqD_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing protein in a pyrroloquinoline
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_KI343_RS15735_PqqD_RRE.1"
                     /evalue="2.40E-09"
                     /identifier="RREFam006.1"
                     /label="KI343_RS15735"
                     /locus_tag="KI343_RS15735"
                     /protein_end="84"
                     /protein_start="6"
                     /score="27.8"
                     /tool="antismash"
                     /translation="KHIIFHEEKNESMLINFVDNYMIGLDQVSTLIWKELMKDRDEIEI
                     INYLAEKYQEDKKLLYKDVKEFLNDLEQAGVIK"
     aSDomain        complement(203..451)
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_KI343_RS15735_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="1.00E-13"
                     /identifier="RREFam002.1"
                     /label="KI343_RS15735"
                     /locus_tag="KI343_RS15735"
                     /protein_end="84"
                     /protein_start="1"
                     /score="42.1"
                     /tool="antismash"
                     /translation="AIKINKHIIFHEEKNESMLINFVDNYMIGLDQVSTLIWKELMKDR
                     DEIEIINYLAEKYQEDKKLLYKDVKEFLNDLEQAGVIK"
     gene            complement(1065..1253)
                     /locus_tag="KI343_RS20650"
                     /old_locus_tag="J11TS1_31780"
     CDS             complement(1065..1253)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037932.1"
                     /locus_tag="KI343_RS20650"
                     /note="Built to rescue LMOF2365_14255 during structural
                     annotation change regression; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="J11TS1_31780"
                     /product="bacteriocin-like WGxF protein"
                     /protein_id="WP_212927382.1"
                     /transl_table=11
                     /translation="MKAISNVILNCIILVIALVVHRLVIRFFDLPINNTLFYWGSLIVL
                     FGVINVFIVLIITPMRK"
     misc_feature    1295..1305
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    complement(1320..1333)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 21.49"
                     /tool="antismash"
     gene            1654..1794
                     /locus_tag="KI343_RS15745"
                     /old_locus_tag="J11TS1_31790"
     CDS             1654..1794
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KI343_RS15745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="J11TS1_31790"
                     /product="hypothetical protein"
                     /protein_id="WP_212927383.1"
                     /transl_table=11
                     /translation="MAEEFLKTYFHFYQYKEVDTKEFIRFTEYYFDEELDSFFKKYNIF
                     K"
     gene            complement(1864..2511)
                     /locus_tag="KI343_RS15750"
                     /old_locus_tag="J11TS1_31800"
     CDS             complement(1864..2511)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248428.1"
                     /locus_tag="KI343_RS15750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J11TS1_31800"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_212927384.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.5e-07, bitscore:
                     22.0, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 7.9e-07,
                     bitscore: 20.3, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIVVIGANGQIGQHLVQQLHAHDQHEVTAMVRKEEQQKEFEDKG
                     IHAVLADLEGSIADLEKVLKGADAVVFTAGSGGKTGPDKTLLVDLDGAVKSIAAAKNAN
                     VNRFVMVSAFQAHNRDSWAESPIKPYMVAKHFADEELVRSGLDYTIIRPGGLQNEPGTG
                     KVQASENLERGNIPREDVAATIVEVLDAKNTYQKGFDLTAGEDSISDAVKQI"
     gene            complement(2695..3510)
                     /gene="pstB"
                     /locus_tag="KI343_RS15755"
                     /old_locus_tag="J11TS1_31810"
     CDS             complement(2695..3510)
                     /EC_number="7.3.2.1"
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="pstB"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010648617.1"
                     /locus_tag="KI343_RS15755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J11TS1_31810"
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="WP_280530145.1"
                     /transl_table=11
                     /translation="MTKKINLQLVRNERREPAVQKEIVYDTKDLNLWYGETHALKNINM
                     DIKEKEVTAIIGPSGCGKSTYLKTLNRMVELVPIVKTSGEITYKGRNILDKSFRVEDLR
                     TQVGMVFQKANPFPKSIYENIAYGPRIHGINNKKILDEIVEKSLRGAFIWDELKDRLHD
                     NAYGLSGGQQQRLCIARCLAVEPNVILMDEPTSALDPKSTLRIEELVQELKKDYAIVIV
                     THNMQQAARISDKTAFFLNGEIIEYDQTDHIFNTPTDERTGDYISGRFG"
     gene            complement(3558..4448)
                     /gene="pstA"
                     /locus_tag="KI343_RS15760"
                     /old_locus_tag="J11TS1_31820"
     CDS             complement(3558..4448)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="pstA"
                     /gene_functions="transport (smcogs) SMCOG1214:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011067531.1"
                     /locus_tag="KI343_RS15760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J11TS1_31820"
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="WP_212927386.1"
                     /transl_table=11
                     /translation="MTTKYMDAELVGKRMSSRVRKNAGWKGVFFASTLFGLFVLAVLLI
                     RVFTQGLEYINIDFLMNHLSTDASRAGIMGAILGTAWLMLIVVPVTMVVGVGTAIYLEL
                     YAKKGKLQSFIATNISNLAGVPSIVYGLLGLTLFVRALEFGNVLLAGGLTLALLVLPIV
                     IVAAQEALRAVPGSLSEASYGMGATKWQTVRRIILPAAIPGILTGAILSLSRAIGETAP
                     LTVLGIPALLIPFPSGIFDTFTALPMQIYYWTIDSSLVAEYANLAAATIIVLLILLFLL
                     NSIAIFIRNKFQQRY"
     gene            complement(4450..5394)
                     /gene="pstC"
                     /locus_tag="KI343_RS15765"
                     /old_locus_tag="J11TS1_31830"
     CDS             complement(4450..5394)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="pstC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010096474.1"
                     /locus_tag="KI343_RS15765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J11TS1_31830"
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="WP_212927387.1"
                     /transl_table=11
                     /translation="MATDNKNRISVSEMIEEQKKKRKFLNQLEKLIPTLLLIIASLSVL
                     TTVGIVYTLLAESVAFFRNVPILDFFTGTILKPLSQNPEFGVLPLLNGTIISTVIAMLV
                     AGPIGMMSAIYLSEYAPDHVRRVLKPLLEILAGVPTIVYGFFAFTFVTPLLRMVFPSIE
                     ATNILSPGIIMGVMIIPMIASLSEDAMSSVPNSIREGALALGSTKLETTFKVVIPAALS
                     GIIASFVLGISRAIGETMIVAIASGSSKNFTFDITQSMQTMTAYIVEVAGGEAAAGTTI
                     YYSLYAVALTLFVFTLIMNLLAKYISRKFREEY"
     gene            complement(5473..6432)
                     /locus_tag="KI343_RS15770"
                     /old_locus_tag="J11TS1_31840"
     CDS             complement(5473..6432)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011067533.1"
                     /locus_tag="KI343_RS15770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J11TS1_31840"
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="WP_212927388.1"
                     /transl_table=11
                     /translation="MSFKRFTLFFFISVLLFLAACGSSDADSAGNSESDNNEELTGNVA
                     IDGSGTVFPLMARLAEDYMLNEQQGVSIEVSRAGTSAGFEKFLAENGTDFNDASREIKE
                     EEQAKADELGIEVQELKVALDGLTIVTHPDNDWATELTPEQVQGIFLGKYTKWSDIDDS
                     WPDEEIQTYGPNENHGTYEFFYETILEEQDLVGSIDLQQDYSTLVTLVSEDKNAIGFFG
                     FGYYKSNQDKINAVSINFGDGAVEPSLETIAEDGPYADYTRPVFTYLNVNHAKEKPEVL
                     DYARYVLENANHVAGEVGFAPIPNEEIKSQLEAIEGLK"
     misc_feature    6530..6548
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 24.6"
                     /tool="antismash"
     gene            6931..7785
                     /locus_tag="KI343_RS15775"
                     /old_locus_tag="J11TS1_31850"
     CDS             6931..7785
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019156977.1"
                     /locus_tag="KI343_RS15775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J11TS1_31850"
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="WP_212927389.1"
                     /transl_table=11
                     /translation="MGRNLVIQTDFGISDGAVSAMYGVALSVDSTLRIFDLTHDIPQYN
                     IWEASYRLYQTLPYWPEETVFISVVDPGVGTSRRSVVVKTAHNQYVVTPDNGTLTHIKK
                     DIGIKEARIIDETVNRLPQSGESYTFHGRDIYAYTGARLASNTISFDQVGPAIDVKDTV
                     ELTKPEAYIEEDVIYGNIDILDVRFGNLWTNISRELFQKTRIAHGDAFEVTILNNRQQV
                     YKNRMTYGHSFADSRLGEPLLYVNSLDNLGVAINQGSFAKTYHIETGANWRIMIRKAPK
                     INE"
     gene            complement(7903..8592)
                     /locus_tag="KI343_RS15780"
                     /old_locus_tag="J11TS1_31860"
     CDS             complement(7903..8592)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380507.1"
                     /locus_tag="KI343_RS15780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J11TS1_31860"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_212927390.1"
                     /transl_table=11
                     /translation="MAILELKKVQKTFGSGKTKVEAMKETDFKAGKGELIAVIGPSGSG
                     KSTFLTIAGGLQTPTNGEVVINGTALSDIKEKKRAKLRLKEVGFVLQASNLVPYLNVAQ
                     QMHVLDKVKKGNMGREEAEALYQSLGIEHLLYNFPKDLSGGEKQRVAIAKALYTNPSII
                     LADEPTASLDSDRAFEVMGILKEMTKEKQTATIVVTHDTRLINDCDKVYKITDGKMELH
                     EIMRRTT"
     gene            complement(8599..9666)
                     /locus_tag="KI343_RS15785"
                     /old_locus_tag="J11TS1_31870"
     CDS             complement(8599..9666)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017798362.1"
                     /locus_tag="KI343_RS15785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J11TS1_31870"
                     /product="ABC transporter permease"
                     /protein_id="WP_212927435.1"
                     /transl_table=11
                     /translation="MFLAWNEIKNNRLRFTLVIGVLMLISYLVFFLSGLANGLEQLNRE
                     AVDKWEADGVILTEESDLRLQQSILSTDDFDGAGVDEYTELSQLSAIASNGDKKVNVYI
                     FAVEPDSFMMPNVTEGDNFAGRGEVIADDTLKEDGFKIGDELSLSSTDETLEIVGFTDK
                     ARFNAAPVLFTDAETLETLHSRKMTEADEDVVNAFIIRTDNLDDVEAAEALQVVDTETF
                     IENLPGYTEQNLTLTLMIYFLFIISAVVLAIFLYVLTIQKISIFGVMKAQGISSRYLAG
                     SVVAQTFLLSLAGVMIGFVLAVITGLFLPAAVPVAFNYLEMLVYGTVLIVVSIIGAFIS
                     VQTIVRIDPLKAIGG"
     misc_feature    complement(10036..10051)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.67"
                     /tool="antismash"
ORIGIN
        1 ttatcagtta tactagtaga catgggtaag gtctcctctt cttagttttt gtcgtgatta
       61 actaaagggt aggataacct tacctttcct atttattcat cttacacaga ctattgtacg
      121 tcatcttaaa tatcccggat agaataatca aatattatca agttaatata taataatata
      181 aaaatataca tcttcctcat ttttttatta caccagcctg ttctagatca tttaaaaatt
      241 ccttaacatc tttatatagc aacttcttat cttcttgata tttttccgcg agataattaa
      301 ttatttctat ttcgtcccta tccttcatca gctctttcca aatcagtgtt gatacttggt
      361 ctaacccaat catatagtta tcaacaaaat taattagcat gctttcgttt ttttcttcat
      421 gaaaaataat atgtttatta attttaattg ccaattttta attcccctta aaacgaattt
      481 aataaaaaac ctttttgtaa tcgcttacct tttcctttat aattacactt tgatcctttt
      541 acatttaaat aacaccttta atttgtaggc attgtcataa atacatatca attatgtttc
      601 caattaatta aaccctataa actaatatga tttgtacata tcatatttag taattctatt
      661 caacgtaaaa cacacttaaa ttaatactgc aatttttagt tatcaccccc ataaattacc
      721 gatatattaa gcctttttga ttatttaact aaatattcta ttctcttttc taaaaattta
      781 actgtttttc ttacaaatct taataattag ataattacat gttacaatac catttcaaat
      841 ttatcaatta tttttaataa tttgttatat ttatattttc tttggtaata atacaacacc
      901 tttgtcgaaa atggtaagcg tatatcacgt atttaaaaag atgccataaa aacaacttca
      961 aatttgttgt ttttatggca tctgcatgtg aaaaggatgg cttgtaatgt ttgagcactt
     1021 caagaaaatc ttttatttca tatatattat aaatagcgct tgagctattt tctcataggt
     1081 gtaattatta gtacaataaa tacattgatg acaccaaaaa gaacaattag tgaaccccaa
     1141 taaaataaag tattattaat aggtaaatca aaaaatctta taaccaatcg atgtacaact
     1201 aatgcgatta ctaagataat acagttcagt ataacatttg atatagcttt catataattt
     1261 cctcctatag tgagatagtt cgatcagatt gctttgcaat atcactatca tgagtaacac
     1321 aaactagcgt tttcccgttt tcttttaaat ttttaaaaat agagagaatg atatttttat
     1381 ctaaatttcc cgtcggttca tctgcaaaaa taactgtaca gggttttagt aggattcttg
     1441 caattgctaa tctctgttgt tcccgccact taattgatat acttttttat ttaaataatt
     1501 tctgcttaat tgaacattct ctagaacgtc ttcctttaac aatatccggt aaatctgcca
     1561 ttgaatcaat atactgagaa tatgagtaat tacatctacg gaaagtccga ttatatgctg
     1621 tggaaattat ttgaagatag aggtggaata aaagtggcag aggaattttt aaaaacctat
     1681 tttcattttt atcaatataa agaagtagat acaaaagaat ttattcgatt cactgaatat
     1741 tattttgatg aagagcttga ttcctttttt aagaaataca atatatttaa atgacattgg
     1801 ctacctgcat actgaataat cctgaaaagg cgctcacaat aaaatgcggg cacctttcat
     1861 tcattatatt tgctttacag catcactgat agaatcttct ccagcagtta aatcaaagcc
     1921 tttttgatac gtatttttag catcgagcac ttctacgatg gtagctgcta catcttcacg
     1981 agggatgttg ccgcgttcca gattttcact ggcctgaact tttcctgtac ccggttcatt
     2041 ctgtaaaccg ccaggacgaa taatggtata gtctagcccg cttcgcacta attcctcatc
     2101 tgcaaagtgt tttgcaacca tatatggttt aattggactt tctgcccagc tgtcacgatt
     2161 atgtgcctgg aatgcactga ccatgacaaa gcggtttaca tttgcatttt ttgctgccgc
     2221 aattgatttt acagcaccgt ctaaatcaac gagcaatgtt ttgtctggac cagttttccc
     2281 gccggatcct gctgtaaata ccactgcatc tgcacctttt aatacctttt ctaagtcagc
     2341 aatgcttccc tcaagatcag ctaaaacagc gtgtattcct ttatcttcaa attctttttg
     2401 ctgctcctct ttacgaacca ttgccgtaac ctcatgctga tcatgtgcat gaagctgctg
     2461 aacaaggtgt tgtccgattt gtccgtttgc tccgattact actatattca tttcgcacag
     2521 ctcctttata ctattcttct tactacatta aattccctgt ttaacctata tccaaacgct
     2581 gctcctaata aattattata taagaaaaag cataattcta aaaagaatac gcttactttt
     2641 acaaataaaa aaagaatgac ctctaaagcc attcattctc aaacaattct tctcttatcc
     2701 aaatcggcca gagatataat ctccagtacg ttcatctgtc ggggtattaa aaatatggtc
     2761 ggtttgatca tattcaatta tttccccatt taagaagaat gctgtcttat ctgaaatacg
     2821 agctgcctgc tgcatattat gggtgacgat aacaattgca taatcttttt ttaattcttg
     2881 tacaagctct tcaatacgta gagttgattt tggatctaat gctgacgttg gttcatccat
     2941 taaaataaca ttcggttcaa ccgctaagca acgggcaata cataaacgct gctgctgtcc
     3001 gccagataac ccgtaagcat tatcatggag acggtccttt aactcatccc agataaatgc
     3061 accgcgtaag cttttctcca caatttcatc aagaattttt ttgttgttaa taccgtgaat
     3121 tcttggccca taggcaatat tttcatagat tgattttggg aatgggtttg ccttttgaaa
     3181 aaccatgccg acctgggttc gtaaatcttc tacacgaaaa gatttatcta aaatatttct
     3241 tcctttataa gttatctctc cagatgtttt tacaatcggc actaactcta ccatccggtt
     3301 caatgttttc agataggttg actttccaca ccctgatggc ccaataatgg cagtcacttc
     3361 tttttccttg atatccatat taatattttt taatgcatgg gtttctccat accataaatt
     3421 taagtctttt gtatcataaa caatttcctt ttgcactgct ggttctcttc tttcatttct
     3481 tacaagctgt aaattaattt tcttcgtcat tacggtgttc atcagactgt ctcctctcct
     3541 atttagccca tttttgttta ataacgctgt tgaaatttat ttcggatgaa tatcgcgatg
     3601 gagtttagca agaacagcag aatcaataat acaatgatgg ttgccgctgc tagattcgca
     3661 tattctgcta caagcgatga atcaatggtc caataataaa tttgcatcgg caatgccgta
     3721 aaagtgtcaa agattcctga tgggaacggt attaataaag ctggtatacc gagaacggtt
     3781 aatggagctg tttctccaat tgctcgggat aatgataaaa tcgctcccgt taaaataccg
     3841 ggtatggctg ctggcaaaat gatacgccgt actgtctgcc actttgttgc ccccatgcca
     3901 taggatgctt cgctcaggct tcctggcact gcccgtaatg cttcttgtgc tgccacaatg
     3961 acaatcggaa gcacaagtaa agctaaagtt aatccacctg ctaacaacac attaccaaat
     4021 tctaaagctc taacaaaaag tgtcagtcct aataatccat aaacaatgga cggcacacct
     4081 gccaggttag aaatattagt tgcgataaag gattgaagct ttcctttctt tgcataaagt
     4141 tccagataaa ttgctgttcc tacgccaacc accatggtca ctggaacaac aatcagcatc
     4201 agccacgctg ttcctaaaat agctcccata atcccagctc tgcttgcatc tgttgagaga
     4261 tgattcatca aaaagtcaat attgatatat tccaatcctt gggtaaacac gcgaatcagc
     4321 aataccgcca aaacgaataa accaaacaag gtggatgcaa agaaaacacc tttccaccct
     4381 gcgtttttcc gtacacgtga tgacatacgt tttccgacca actctgcatc catatatttc
     4441 gttgtcatct tagtactcct ctctaaactt acgtgaaata tattttgcta atagattcat
     4501 aattaaggtg aaaacaaata gtgttaatgc taccgcataa agactgtaat aaatggttgt
     4561 tcctgctgcc gcttccccgc cggctacttc tacaatataa gccgtcattg tctgcataga
     4621 ctgcgtaata tcaaaggtga aattcttgga gcttccactc gcaatagcta caatcattgt
     4681 ctcccctatc gcccgggaaa ttccaagtac aaaggaagcg ataatcccag atagcgctgc
     4741 tggaataact actttaaatg ttgtctccag cttcgttgat ccaagcgcta aagctccctc
     4801 tcgaattgaa tttggaacag aagacatcgc atcttcggaa agcgaagcaa tcatcggaat
     4861 aatcatgaca cccataatga ttccgggact aagaatattc gtagcctcga tggatggaaa
     4921 aaccatcctt agcaaaggtg ttacaaacgt aaatgcaaag aatccataaa caattgtcgg
     4981 caccccagct aaaatctcta ataacggttt taataccctt cgcacatgat caggtgcata
     5041 ttcacttaaa taaattgctg acatcattcc aattggtccg gctaccagca tcgcaatcac
     5101 tgttgaaatt attgttccat ttaataaagg aaggacgcca aattccggat tttgacttag
     5161 cggttttaat atcgttcctg taaagaaatc tagaatcggt acattcctga aaaatgcaac
     5221 ggattcagca agtaacgtat aaacaattcc tactgtagta agaaccgata acgaagcaat
     5281 aatgagtaac agtgttggta ttaacttttc taattgatta agaaatttac gtttcttttt
     5341 ttgttcttca atcatttcac taaccgaaat ccgattctta ttatctgtag ccatgatgcg
     5401 aaagccacct tcctcgcaga atgaggaaga tgagaagcat aatcctgcac agccctcatc
     5461 ttccttcttt tcttatttta atccctcgat tgcctctaat tgagatttga tttcctcatt
     5521 tggaattggg gcaaatccga cttccccagc aacatgattt gcattttcca aaacatatct
     5581 tgcatagtca agaacctctg gcttttcctt tgcatgatta acattcaagt aagtgaacac
     5641 agggcgtgta tagtccgcat aaggcccatc ttctgcaatg gtttctagag aaggttccac
     5701 tgccccatct ccaaagttaa ttgatacggc attaatcttg tcctgattac tcttatagta
     5761 gccaaacccg aaaaatccaa ttgcgttctt atcttcggaa acaagggtta ctaatgtgga
     5821 gtaatcttgt tgcaggtcaa tacttcctac taaatcttgc tcctctaaaa ttgtttcata
     5881 aaagaattcg tacgttccat gattttcatt tggaccataa gtttgaattt cttcatctgg
     5941 ccaagaatca tctatatctg accactttgt atatttacca aggaagattc cctgtacttg
     6001 ttctggagtt aactctgttg cccagtcatt atctggatgt gtcacaatcg ttaaaccgtc
     6061 taatgcaact ttcaactctt ggacttcaat tcctaattca tctgcttttg cttgttcttc
     6121 ttcttttatc tctcgcgagg catcgttaaa atctgtacca ttttccgcta agaatttttc
     6181 aaagcctgcg cttgttccag ctcgacttac ttctatcgaa acaccttgct gctcatttaa
     6241 catataatct tcagctaaac gcgccattaa tgggaatacg gtaccagaac catcaatcgc
     6301 aacattccct gttagctctt cgttattatc agactccgaa ttgccagcac tgtctgcatc
     6361 acttgaaccg caagctgcta aaaatagtag aacggaaata aaaaagaata aagtaaaacg
     6421 tttaaagctc atgaaagggc ccccttagat ttaatatcat tatttactgg acatttcctc
     6481 tctgtcctta ctttgaagta tagagaagaa ttattaatat agtttgttgt aattgtaaag
     6541 agtatgtaaa ttaactcctt tatttttaaa gaggggctaa gctcgtccta ttccatacga
     6601 caaacaaaat catatacttc tattattaat ctatgcacag atatccaatt tatcattcga
     6661 attaacaacc tgtagaaaaa gcgtatacaa gttaaaacaa aaccaggcat ttagactttt
     6721 atcattttca aagaaacttg cgcttatact gtactctctg cattctttct tatcgtgcaa
     6781 aataagtact tgtttttcaa attttcgtta cttataatga taagtcctta tcgatatttc
     6841 ggttgaggcg tggttttttt tgtttttaag aagataaaaa gcggccacac attcatgaaa
     6901 atgggaatac tatagatgag ggggacgtat atgggcagaa acctagtaat tcaaacagat
     6961 tttggcatta gtgacggggc agtcagcgct atgtacggtg tggcactttc tgtagacagc
     7021 acgcttcgga tattcgatct gacgcacgac attccgcaat ataatatttg ggaggcatcc
     7081 tatcgtcttt atcaaacgtt gccttattgg cctgaagaga ccgtttttat ttctgttgtc
     7141 gacccggggg ttggaacttc cagacggagt gttgtagtta aaacggcgca taatcaatat
     7201 gttgtgacgc ccgataacgg gacactcact catattaaga aagatatcgg aattaaagaa
     7261 gcaagaatca ttgatgagac agtaaatcgc ttaccccaat caggagaatc ttatactttt
     7321 cacggccgtg atatttatgc atataccgga gcaagattgg cttccaatac cattagcttt
     7381 gaccaagtcg gccctgccat tgatgtgaaa gatactgtgg aattaacaaa accggaagcc
     7441 tatattgaag aagatgttat ctacggtaat atcgatatat tagatgttcg ttttgggaat
     7501 ctctggacaa atattagcag agagttattt caaaagacga ggattgcgca cggggatgcc
     7561 tttgaagtta ccattcttaa caatcgccaa caagtataca aaaataggat gacatatgga
     7621 cattcttttg ctgacagccg acttggcgag cctttgttat acgttaattc attagataat
     7681 ttaggagtag caatcaatca gggctctttt gcaaagacct atcacattga aacaggcgct
     7741 aattggcgaa ttatgattcg taaggcaccg aaaatcaatg aatagcaggt tgctccaccg
     7801 aggtgctcca tccatatctt taaagaaaaa agtgatgcaa ttgctatgaa actagctgta
     7861 catcactttt ttcatggatt gattatttct atataattgc attcacgtcg tacggcgcat
     7921 tatctcatgc aactccattt taccgtctgt tattttatat actttgtcac agtcattgat
     7981 cagtcttgta tcatgtgtca cgacaatggt tgctgtttgt ttttctttcg tcatttcttt
     8041 caatataccc atgacctcaa atgcccggtc ggagtctaga gaagcagttg gttcatcagc
     8101 taaaataata ctcggattcg tgtaaagcgc tttagcaatc gcaactcgct gtttttcgcc
     8161 gccagataag tctttcggaa agttataaag cagatgctct attcctaatg attgataaag
     8221 cgcttcagct tcttctcttc ccatattccc ttttttcacc ttatctaata cgtgcatttg
     8281 ctgcgctaca tttagataag gtaccaaatt agacgcttgt aaaacgaaac cgacttcctt
     8341 caatcttaat ttcgcacgct ttttctcctt tatatctgac aaagcagttc cattaatgac
     8401 tacctctcca tttgtcggtg tttgcagtcc gccggcaatg gttaaaaacg tacttttacc
     8461 agaaccagag ggaccaataa cggcaattaa ttcgcctttt cccgctttaa aatccgtttc
     8521 tttcattgcc tctacttttg tttttccaga tccaaatgtt ttctgcactt ttttcaattc
     8581 taaaatagcc atgccttctt aacctcctat cgcctttaac ggatcaattc gtacgatagt
     8641 ctgtacagag ataaatgcac cgattatcga aacaacaatc aggacggttc cgtacaccag
     8701 catttctagg taattaaagg ctactggaac agctgccggc aaaaacagtc ccgtgatgac
     8761 agcaagaacg aagccaatca tgactccggc taacgaaagc aaaaatgtct gcgccaccac
     8821 agacccggcc aagtatctgg atgatattcc ctgcgccttc attactccaa aaatacttat
     8881 cttctgaatc gtaagtacat acaggaaaat tgctaaaaca actgcagaaa taataaatag
     8941 gaaataaatc atcaacgtta aagttaaatt ctgttcggta taacccggca gattttctat
     9001 aaatgtttct gtatccacaa cctgcaacgc ttcagctgct tccacatcgt ccagattatc
     9061 tgtacgaata ataaaggcat taaccacatc ctcatccgct tcagtcattt tcctagaatg
     9121 aagggtttcc agcgtttccg catctgtgaa cagtacaggc gctgcattaa atcttgcttt
     9181 atccgtaaaa cccactattt ctaatgtttc atccgttgaa gataaggaaa gctcatcacc
     9241 aattttaaag ccatcttctt tcaatgtatc atcggcaatg acttctcctc tgcctgcaaa
     9301 gttatctccc tctgtcacat tcggcatcat aaaagaatcc ggttctaccg caaagatata
     9361 tacatttact tttttatctc cattactggc aatggcactt agctgggata attcggtata
     9421 ttcatccacc ccggccccat caaaatcatc ggtagacaag atggactgct gcaggcgcaa
     9481 atccgattct tctgttaaaa taacgccgtc tgcctcccat ttatccaccg cttcccgatt
     9541 taattgttcc aaaccattgg ccagcccaga taaaaagaaa acaagataag aaatcagcat
     9601 caatacgcca atcactagtg taaaacgcag cctattattc tttatttcat tccaagctaa
     9661 aaacatcgca ccacttccct ttcctttcat ttacacatcc cgttgcctta caaattcagg
     9721 aatggccaca aaagaactgt cgaaaacctt aaacgttgtg aaatactgcc ttgcttttga
     9781 cacatactac atatgcaggc tacatcagcc tcctttcttt catgccgaac atttcatata
     9841 taaatttttt gaagttatac gtgtctcctc ctatttaaaa gtggtattat agtatatgtt
     9901 ttcggaaata caaataggca gatcctattt tttggagtga ttacattgaa gacatggtta
     9961 aaaaatcgga caaaggcgca ggtaattacc gttggtttat tgctaattgg ttttatagcg
    10021 tctatgattt ttatcaataa taattatgat ttatatgata gaacgattgt ccaaataacc
    10081 gatgtgcaag aaataaatag agaagaacaa gtagatacac atggaaatga agacgaacat
    10141 gtcacacagc atatcgtcgg ggaaataaag aatggatccg ataaaggcca agtaactgcg
    10201 tttgataatg aatatatcca atccctggca ttagactatg aattagctgc tggagatgag
    10261 atttttctag cgcaagatgg gaaagcgaca ggagatatta aacgagataa atatttcgtt
    10321 tttgtagcat ggatattctt gattatttta ctggctgtcg gcaaatccag aggtgcctta
    10381 tctgcgctta gtctcctggt caatgcagtc atcctgtcca tcgcattaga catctatatt
    10441 cataacccta gtat
//
