LOCUS       NZ_BOQQ01000006        10405 bp    DNA     linear   CON 16-SEP-2024
DEFINITION  Shouchella clausii strain J1TS1 sequence06, whole genome shotgun
            sequence.
ACCESSION   NZ_BOQQ01000006
VERSION     NZ_BOQQ01000006.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Shouchella clausii
  ORGANISM  Shouchella clausii
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Shouchella.
REFERENCE   1
  AUTHORS   Okamoto,M., Kumagai,M., Kanamori,H. and Takamatsu,D.
  TITLE     Antimicrobial resistance genes in bacteria isolated from Japanese
            honey, and their potential for conferring macrolide and lincosamide
            resistance in the American foulbrood pathogen Paenibacillus larvae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 350954)
  AUTHORS   Okamoto,M., Kumagai,M., Kanamori,H. and Takamatsu,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2021) Contact:Daisuke Takamatsu National
            Agriculture and Food Research Organization; 2-1-2 Kannondai,
            Tsukuba, Ibaraki 305-8602, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.2
            Genome Coverage                   :: 1380x
            Sequencing Technology             :: Illumina NovaSeq 6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018332735.1-RS_2024_09_16
            Annotation Date                   :: 09/16/2024 05:58:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,326
            CDSs (total)                      :: 4,235
            Genes (coding)                    :: 4,197
            CDSs (with protein)               :: 4,197
            Genes (RNA)                       :: 91
            rRNAs                             :: 5, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 1, 5, 3 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 14 of 38
            Pseudo Genes (incomplete)         :: 28 of 38
            Pseudo Genes (internal stop)      :: 6 of 38
            Pseudo Genes (multiple problems)  :: 9 of 38
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:04:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 155186
            Orig. end                         :: 165591
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BOQQ01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(1025..1834)
                     /locus_tag="KI354_RS13220"
                     /old_locus_tag="J1TS1_26630"
     CDS             complement(1025..1834)
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1069: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245201.1"
                     /locus_tag="KI354_RS13220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J1TS1_26630"
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_011245201.1"
                     /transl_table=11
                     /translation="MKVNGWHLLLAICLVLSFFPVLTMAATAFLETSQLFAGGLQLLPI
                     PPTLDHFSQVWESIPILRYMGNSFFVATVVTLIQLFTSILAAYGFTQFQFRGREFCFYL
                     VIASIFIPIQVTMLPNYILLSDLELVNTYFGLIVPQIANGIGVFLLRQSFRSVPASLIE
                     SARVDGAGDWRRLWKILFPSVKPSVIALGILFFITTWNEYLWPLLMINDNDLMTMPLAL
                     QEFISAEGGTSWGPMMAVAFLASVPPLAVYLLLQRHIMSSFLHTGVK"
     gene            complement(1824..2726)
                     /locus_tag="KI354_RS13225"
                     /old_locus_tag="J1TS1_26640"
     CDS             complement(1824..2726)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1067: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245200.1"
                     /locus_tag="KI354_RS13225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J1TS1_26640"
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_244835548.1"
                     /transl_table=11
                     /translation="MKMKPASGWLQRIKPLLFIAPAMLPIVLFIFVPLVRSIYWSFLEW
                     NMVSPTKTWVGLDNYVELFSNREFLNASANTLWYMGMLLVLILFVPYMTAYGVTQVSGR
                     WQHFYRGALFVPHVLSLAVASVIFLWLYNPLIGSVNEILRFFQLPDVNWLNDANWALFA
                     ITLVVAWNTFGYHFIVLLAGILSVPEEVKESARVEGMKSKLGMLHRIVFPLTGPTILFV
                     FVLTTVMGIQFAFVPIQMLTNGGPDQSTSNLVFLTYQYGFQFFRTGLGSATAVLTFLFF
                     VLIILLQAKVLDRRVHYES"
     gene            complement(2723..3520)
                     /locus_tag="KI354_RS13230"
                     /old_locus_tag="J1TS1_26650"
     CDS             complement(2723..3520)
                     /GO_function="GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013429.5"
                     /locus_tag="KI354_RS13230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J1TS1_26650"
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="WP_095327246.1"
                     /transl_table=11
                     /translation="MSVYINLLPFMKKINDAPAFLQDWGGGIEISTDGPHWHGDTDWAQ
                     EKERFASYAGDIGVHLPIWELNLASVQFPQYAAYSFEAYRTYLEWASTFASHAVLHTHL
                     YNMPLFHREEAQALSKHYIKQLGDIAEQKGIMLLVENVGFHDKMLFDEREFVQLFEEIP
                     AIQALLDVGHATINNWDIANVIEALGTRLSALHLHDNDGENDLHLPIGAGVTRWNEIWQ
                     AVAEQTHSLRLILEYDESASLEVLKRHGNELLQAGKVVGKINV"
     misc_feature    3481..3494
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.72"
                     /tool="antismash"
     gene            complement(3539..4837)
                     /locus_tag="KI354_RS13235"
                     /old_locus_tag="J1TS1_26660"
     CDS             complement(3539..4837)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1143: sugar-binding lipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245198.1"
                     /locus_tag="KI354_RS13235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J1TS1_26660"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_011245198.1"
                     /transl_table=11
                     /translation="MWKKHAALLSTLGTVTVLAACGGTEGATTSEEKVTIEYWHVNNED
                     WGGKAIKELVEQFNASQDRIEVKESFQPGNYQGLLQNAQAALSGGNPPDVAQIGYNYIA
                     YVDQNVPYVPVSELAANDEEGEAFLNESYLDNIKALGYSNDKETLAGLPYALSNPIMYV
                     NADLLKEAGWNVDELPQTWDEMQEMAKMVKENTGEYGLYVQQPPDSWAEYSLVRSNGGD
                     FLDFSSDEVVSTIGSPESVEVFDMLGRMIEDQHALHVTWEEGIQAFATGKVGMMMTTVG
                     RRAAIEEQSNFDLRSVEIPTFGDKERQVAAGGNALFSFSQDPEKQAAAWEFMKYLSSPE
                     AMEIWVEGTGYLPPVKGLAEDPDTLGPFFDENPIMQTANRQMESIVPWINFPGANGLQA
                     EQAILDARDQIFDGADAESVLKETESKINGYIQ"
     misc_feature    4921..4938
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 18.49"
                     /tool="antismash"
     gene            complement(5001..5405)
                     /locus_tag="KI354_RS13240"
                     /old_locus_tag="J1TS1_26670"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245197.1"
                     /locus_tag="KI354_RS13240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J1TS1_26670"
                     /product="ectoine synthase"
                     /protein_id="WP_212930020.1"
                     /sec_met_domain="ectoine_synt (E-value: 8.2e-50, bitscore:
                     162.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVVKLADIIGTNRDVDGGNWRSQRIVVESDGMGYSLHDTQIKAG
                     TETHLWYKYHLESVYVIEGEGEVETVKDGKVWPVKQYECYVLDKNDEHLLRAKTDMRMV
                     CVFNPPVTGEEVHDEDGAYPLPEHIKPLNS"
     gene            complement(5442..6722)
                     /gene="ectB"
                     /locus_tag="KI354_RS13245"
                     /old_locus_tag="J1TS1_26680"
     CDS             complement(5442..6722)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (25-358). E-value: 1.2e-81.
                     Score: 266.3. Matches aSDomain:
                     nrpspksdomains_KI354_RS13245_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245196.1"
                     /locus_tag="KI354_RS13245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J1TS1_26680"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_011245196.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.4e-83, bitscore:
                     273.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNETMSIFNELESEVRSYCRSFPAVFTKAKGWKMWDEDGTEYIDF
                     FAGAGALNYGHNDEKMKRALIDYIADDGITHSLDMATKPKAAFLKKLNEVILKPRKLNY
                     KVMFPGPTGTNTVESALKLARKVTGRTEIISFTNGFHGMTIGSLSVTGNASKRKGAGIP
                     LTNVVTMPYDKFGDEEVDTLRYLEQFLHDNGSGVDIPAAVILETVQGEGGINAARMEWL
                     QQLERICKKYDILMIIDDVQAGIGRTGTFFSFEEAGITPDIVCLSKSIGGYGLPLALTL
                     FKPELDIWAPGEHNGTFRGNNHAFITATAALSYWEDPSFEQAIKDKSKKIHAFLTKLVD
                     SYPEMDGCVKGRGFMAGIGSKVDGLAGKVAAEAFKRGLIMETAGPEDEVFKLFPPLTIS
                     DEDLEKGFAIIEESVRAVLGVKEPVTT"
     aSDomain        complement(5649..6647)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KI354_RS13245_Aminotran_3.1"
                     /evalue="1.20E-81"
                     /label="KI354_RS13245_Aminotran_3.1"
                     /locus_tag="KI354_RS13245"
                     /protein_end="358"
                     /protein_start="25"
                     /score="266.3"
                     /tool="antismash"
                     /translation="FTKAKGWKMWDEDGTEYIDFFAGAGALNYGHNDEKMKRALIDYIA
                     DDGITHSLDMATKPKAAFLKKLNEVILKPRKLNYKVMFPGPTGTNTVESALKLARKVTG
                     RTEIISFTNGFHGMTIGSLSVTGNASKRKGAGIPLTNVVTMPYDKFGDEEVDTLRYLEQ
                     FLHDNGSGVDIPAAVILETVQGEGGINAARMEWLQQLERICKKYDILMIIDDVQAGIGR
                     TGTFFSFEEAGITPDIVCLSKSIGGYGLPLALTLFKPELDIWAPGEHNGTFRGNNHAFI
                     TATAALSYWEDPSFEQAIKDKSKKIHAFLTKLVDSYPEMDGCVKGRGFMAGI"
     gene            complement(6752..7246)
                     /gene="ectA"
                     /locus_tag="KI354_RS13250"
                     /old_locus_tag="J1TS1_26690"
     CDS             complement(6752..7246)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018923355.1"
                     /locus_tag="KI354_RS13250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J1TS1_26690"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_244835549.1"
                     /transl_table=11
                     /translation="MVQTPVLTLTKPTVEDGAAMWQLAKSSSLDTNSSYKYIMMCEYFD
                     DTCIVAKDGDEVVGFITAFIPPKQQDTLFIWQIGVAASQRGRGLGLDLINKLIEREACK
                     DIRYVEATVTPSNKASQALFRKLARSHQTDCQVSSCFSEQLFPGNSHEQENTFRIGPLR
                     S"
     misc_feature    7316..7329
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.37"
                     /tool="antismash"
     gene            complement(7680..8090)
                     /locus_tag="KI354_RS13255"
                     /old_locus_tag="J1TS1_26700"
     CDS             complement(7680..8090)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245194.1"
                     /locus_tag="KI354_RS13255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J1TS1_26700"
                     /product="hypothetical protein"
                     /protein_id="WP_095327243.1"
                     /transl_table=11
                     /translation="MYNGHDYPMRFSPGTFPFPPTQGGPPGPPPTQGGFFPGQGGPPTT
                     SPGQGTGAPPGPPPSQAALSTLGAPSVFAVDPGAIAGCLFRYTFIRLNNGESFWFYPTF
                     VGRRSVAGWRWFFFRWVFFGIDTERIASFQCV"
     gene            8235..9686
                     /locus_tag="KI354_RS13260"
                     /old_locus_tag="J1TS1_26710"
     CDS             8235..9686
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015292.5"
                     /locus_tag="KI354_RS13260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="J1TS1_26710"
                     /product="spore germination protein"
                     /protein_id="WP_212930022.1"
                     /transl_table=11
                     /translation="MRTTDQNACAAAIKSAFYQSNSVQTLEIPLLETDQALVLFIQEIV
                     DQNKLHALIVDPLTRFRLAKQSYPTESVTTWLKDGTPLPNATVEQIKREMMNGQAVVLL
                     KNEQPVLIDCAMWNVRSVSVPLTSSVYEGPASALTESLDVNMNLLRNYFRSPELAIETL
                     EVGENAAKKMAIVSLHGKTNPDLVKEVRERLKQVHVAEFIISQIATDALEGKGFLFPRT
                     MANDRPDACALALAKGKIVLLVEGSPLAILAPSLFFHFFQNQDDYLSEFGRFGARPLRY
                     LYFFIATFFPAITVAIIRFHLDHIPTEVANQLVKTHETLAPYTIELVFVILLLQLIMDG
                     SYRLPGNTIFAVTFIGTMLISDIATNVSLFHPITIVVIGVCYITSFPVLNRGLLSPIFF
                     IRITLIIVAHFFGFAGMFAVTTAVIIHMAHLRSLGIPYLYPFIPFQPDKWHDTIFRPTL
                     TMAINKPTPLPFKKAEAAQNRRGSY"
     misc_feature    complement(10340..10360)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 22.38"
                     /tool="antismash"
ORIGIN
        1 acttcaatcc gattgccggc ttcatcaaaa aagtgaagat gctctggcgg aatcgagaga
       61 tcgacttttt gcccggttgt ataagattct gtccctgcca cgacttggat agtgacgcca
      121 ttgatggtga ggtaatggac atagcgtgaa ccaatgtatt ccttgcgaac atattccccg
      181 tgaaggacac acgcttctgg gagcttctta aacataatgt gctcgggcct tacacccatt
      241 tctgcaactc cgggaatcat cgccctgtcg gcaaatgcat cggctggcag catattcatt
      301 ggcggcgtgc cgataaaagt ggcgacaaat cgtgttttgg gcttttcgta aatgtcttca
      361 ggcgtcccaa tttgttcgat aacaccgcca ttcatcacgg cgatccgctg tccaattgtc
      421 attgcctcta attggtcgtg ggtgacatag accatcgttt gtttcaagcg gttatgaagt
      481 tcgattaagt cattgcgtgc ggacacacgc aaaagcgcat caagattgga caacggttca
      541 tccaacagga aaaatggcgc ttgtttaacg accgctcggg caagggccac ccgttgacgt
      601 tggccgccgg aaagttcggc tggtttgcgc tctaaatacg gctttagctc gactaagccg
      661 gcggcgtatt caactgcttc gcgtatgtct tgctttttct ttttgcgaat cgtgagcgaa
      721 aaagcgatgt tatcaaacac ggtcatatgg ggataaaggg catagttttg gaaaaccatt
      781 gccacattgc gatcggcaac gtcaatccct gccattgctt gcccgcccat taaaatcgtg
      841 cctgacgtag gctgttccag acccgcgatc agtcgcaaca atgtggactt gccgcatcca
      901 gaaggaccaa gaagcacaag gcgttcgccg ctgttaatca ttaaatccat tggcggaatg
      961 acatttgtct gcccaaagtt tttctctact tgtgtaaaca caatgctttt atcttctttc
     1021 atcgctactt cacccctgta tgcaaaaagc tgctcataat atgtcgctgc aacaacaaat
     1081 acacagcgag tggcgggacg gacgcaagaa atgcgacagc catcatcggc ccccacgatg
     1141 tgcccccttc tgcacttata aattcttgca aagcgagagg catcgtcatc aagtcgttgt
     1201 cgttaatcat tagtaacggc cataaatatt cgttccatgt cgtaataaaa aagagaatgc
     1261 ctagagcaat caccgatggc ttaaccgacg ggaataaaat tttccaaagc ctgcgccaat
     1321 cccctgcccc gtccacgcga gcagactcaa ttaggctggc aggaacagac cgaaacgatt
     1381 gccttaacag gaaaacgccg atcccatttg cgatttgcgg cactattaaa ccgaaatagg
     1441 tatttacaag ctctaaatca cttaacagaa tatagtttgg cagcatcgtc acttgaatag
     1501 gaatgaagat gctcgcaata accaagtaaa agcaaaactc tcttccacgg aattgaaatt
     1561 gagtaaaccc gtaagcagca aggatgctag tgaacagttg tatcaatgta accactgttg
     1621 cgacgaagaa agagttgccc atatagcgta aaatcgggat cgactcccac acttggctaa
     1681 aatggtctaa tgtaggcgga atcggcaata attgcaaacc acctgcaaac aactggcttg
     1741 tttcaagaaa agcggttgcc gccattgtca aaaccggaaa aaaactaagg accaggcaaa
     1801 tcgccaataa caaatgccat ccgttaactt tcataatgga ccctccgatc aagcacttta
     1861 gcctgaagca aaatgatcag aacaaaaaac aaaaacgtta acacggcagt tgctgaccca
     1921 agtcctgtgc gaaaaaactg gaacccatac tgatacgtta agaacaccaa attgcttgtt
     1981 gattggtctg ggccgccatt ggttagcatt tgaatcggca caaaagcaaa ttggatgccc
     2041 attactgtcg ttaacacaaa tacgaacaaa attgttggtc ctgtaagcgg aaaaacaatc
     2101 cggtgcaaca tgccaagttt gcttttcatt ccttctacac gggccgattc cttcacttct
     2161 tcagggacac ttaaaattcc agcaagcaac acaataaagt gatagccaaa cgtattccag
     2221 gccacgacaa gcgtaatcgc aaacagcgcc caattggcat catttagcca gttgacatcg
     2281 ggaagttgaa agaaacggag aatttcattc actgagccaa tcaatggatt gtataaccat
     2341 aagaagatga cagacgcaac cgcaagggaa agcacgtgcg gtacaaaaag agcgccacga
     2401 taaaaatgtt gccaccgccc tgaaacttgc gtgactccgt aggctgtcat gtaaggaaca
     2461 aacaagatta ggacgagcag catgcccatg taccatagcg tattcgctga tgcgtttaaa
     2521 aactcgcgat ttgaaaacaa ttcgacgtaa ttgtctaaac cgacccatgt tttggttgga
     2581 ctaaccatat tccattctaa aaagctccaa taaatcgagc gaactagagg aacgaaaata
     2641 aagagaacaa tcggcagcat cgcaggagcg ataaacaaaa gcggctttat ccgctgcagc
     2701 caaccgcttg ctggtttcat cttcatacgt ttatcttccc aaccactttt cctgcttgta
     2761 aaagctcgtt gccgtggcgc ttcaacactt ctagtgatgc tgactcgtca tattctaaaa
     2821 ttaatcgcag agagtgtgtt tgctcggcaa cggcctgcca aatctcattc cagcgtgtca
     2881 ctcctgcgcc aattggcaaa tgcaagtcat tttcgccgtc attatcatgc aaatgaagag
     2941 cagacagcct tgtccctagc gcttcaatca catttgctat atcccaattg ttaattgtgg
     3001 catggccgac atcaagcaat gcttgaatcg ccggaatttc ctcgaacaat tggacaaatt
     3061 cccgttcatc aaacagcatc ttatcgtgaa aaccgacatt ttcaaccaat agcatgatgc
     3121 ccttttgttc agcgatatca ccgagctgtt ttatgtaatg cttcgataaa gcttgtgctt
     3181 cttctcggtg aaacaaaggc atgttgtata gatgagtgtg gagcacagca tggcttgcaa
     3241 atgtagaagc ccattctaag tacgtacggt atgcttcaaa ggaataggcc gcatattgag
     3301 ggaactggac agaggccaag tttaattccc aaattggcaa gtggacgcca atgtctcctg
     3361 cataggaagc aaaccgctct ttctcctgag cccaatcggt atcgccatgc caatgggggc
     3421 cgtctgtcga gatttcaatt ccgccacccc aatcttgtaa aaaagctggg gcatcgttaa
     3481 tttttttcat aaaaggaagc aagttgatat atacactcat tgtatgtccg cctctcgctt
     3541 attgaatata tccattaatt tttgattctg tttctttcaa aaccgattcc gcatcggcac
     3601 cgtcaaaaat ttgatccctt gcatctagaa tcgcttgctc tgcctgcaaa ccatttgcac
     3661 ccggaaagtt gatccaaggg acaatcgatt ccatttgccg attagcggtt tgcatgatcg
     3721 gattttcgtc aaaaaaagga cctaacgtat ctggatcttc tgccagtccc ttgaccggcg
     3781 gtagataacc agtcccttca acccaaattt ccatggcttc tgggcttgat aaatacttca
     3841 taaattccca cgccgctgcc tgtttctctg gatcctgtga aaatgagaac agcgcattcc
     3901 cgccagcagc tacttggcgc tctttatccc caaaggttgg aatttcgacg gaacgcaaat
     3961 caaagttgct ttgttcttca atcgcagccc ggcggcctac ggttgtcatc atcatcccga
     4021 cttttcctgt ggcaaatgcc tgtatgcctt cttcccacgt cacatggagg gcatgttggt
     4081 cttcaatcat gcgtccaagc atatcaaaca cttccacact ttccggggaa ccgatggttg
     4141 atacaacttc gtcgctacta aaatcgagga aatccccgcc atttgaacga acaagtgagt
     4201 attccgccca gctatctggg ggctgttgaa catagaggcc gtactcccct gtattttcct
     4261 ttaccatttt ggccatttcc tgcatctcat cccatgtttg cggcagctcg tctacattcc
     4321 agccagcttc ttttagcaaa tctgcattga catacataat tggattgctt aaggcataag
     4381 gcaatccagc caatgtttct ttatcgttgg aatacccaag cgctttaata ttgtcgagat
     4441 aagattcgtt taaaaacgct tccccctctt catcattagc cgctagttcg gacacaggga
     4501 cataaggcac gttttggtcg acataagcta tgtagttgta gccaatttgc gcgacatctg
     4561 gtggattccc tccacttagc gccgcctggg cattttgcaa cagcccttga taatttccag
     4621 gctgaaaaga ttcctttact tcaattcgat cctgggaagc attaaactgt tcgaccagct
     4681 ctttaattgc tttgccgccc cagtcttcgt tgtttacatg ccaatactca atcgtgactt
     4741 tctcttcact cgtggttgcc ccttccgtgc cgccacaagc tgctaaaaca gtgaccgtgc
     4801 ctagtgtcga taacaaggct gcatgtttct tccacacagt ctctccaccc ctttgttggt
     4861 taaatacatg tctagtataa agacaggcta ttaatttcta atgagaggga agcaaatatt
     4921 ttgtaaaacg tttgtaaagg aagcggacaa aaaaaagaca aagagccatt gccctttgtc
     4981 gatttcaatc ttttcccgct ctatgagttt agaggcttta tatgttctgg aagaggatag
     5041 gcaccgtctt cgtcatgaac ttcttcgcct gtaacgggtg gattaaacac acagaccatg
     5101 cgcatatccg tcttcgcccg cagtaagtgc tcatcatttt tgtcgagcac ataacattcg
     5161 tattgcttga ctggccatac ttttccatct tttaccgtct ccacttcgcc ttccccttca
     5221 atcacgtaaa ccgattcaag atggtatttg taccaaagat gcgtttctgt gccggcttta
     5281 atttgcgtgt catgcaaaga ataacccata ccgtctgact cgacaacaat ccgctggctc
     5341 cgccaattgc cgccgtctac atcccggttc gtgccaataa tgtctgccag tttgactact
     5401 ttcattttct ctcctccaat gattaagtaa ctaacgcttg tttaagtggt gacaggctct
     5461 tttacgccaa ggacggcgcg tacactttct tctataatgg caaagccttt ttctaagtcc
     5521 tcatctgaaa tggtgagcgg cgggaacagc ttaaagactt catcttctgg accggccgtt
     5581 tccatgatta agccgcgctt aaatgcttca gccgctactt ttccagctag gccgtctact
     5641 tttgaaccga ttccagccat aaagcccctt ccttttacac aaccatccat ttctggatat
     5701 gagtcaacaa gtttcgttaa gaaagcatgg attttctttg acttgtcttt aatcgcttgt
     5761 tcaaacgatg ggtcttccca gtaggacaat gctgccgttg ctgtaataaa ggcgtggttg
     5821 tttccacgga atgtgccatt atgctcgccc ggagcccaaa tatcaagttc aggtttgaaa
     5881 agggtgagag ctaatggcaa tccatacccg ccaattgatt tagacaggca gacaatgtca
     5941 ggcgtaatgc ctgcttcctc gaagctaaaa aacgtccctg tgcgtccaat acccgcctga
     6001 acatcatcaa taatcattaa aatgtcgtat ttcttgcaaa tgcgctctaa ttgctgtaac
     6061 cattccatac gtgcggcatt aatgccgccc tctccttgaa cagtttcgag aatgacggcc
     6121 gccggaatgt ctactccaga gccattgtca tgtaaaaact gttcgagata acgaagtgta
     6181 tcgacttctt cgtctccaaa tttgtcatac ggcatcgtta caacattagt caaagggatg
     6241 cctgcacctt ttcgtttaga agcattccct gttaccgaaa gcgacccgat tgtcattccg
     6301 tggaagccat ttgtaaaact gatgatttct gtcctccctg tcaccttgcg agccagcttc
     6361 agggctgatt caactgtatt tgtgcctgtt ggaccaggaa acatcacttt gtaattgagc
     6421 tttcgtggct ttaaaatcac ttcgttcaat ttttttagga atgctgcttt tggctttgtt
     6481 gccatatcaa gtgagtgggt aattccgtca tcggcaatat aatcgataag agcacgcttc
     6541 attttttcat cattgtggcc ataattgaga gcccctgctc cagcaaagaa gtcgatgtat
     6601 tccgttccgt cctcatccca cattttccag ccttttgcct tcgtgaaaac agccgggaag
     6661 ctgcggcagt aactacgcac ttctgattcc aattcattga aaatgctcat tgtttcattc
     6721 actgtctgtt gttcctcctt cattttttcg tttaagaacg aagtggtcca atgcgaaacg
     6781 tgttttcttg ttcatggcta tttccaggaa ataactgttc cgaaaaacaa ctagacactt
     6841 ggcagtctgt ttgatggctt cgggctaatt tcctgaataa cgcttgcgaa gctttgttcg
     6901 aaggggttac cgttgcttcc acgtaacgga tatccttgca tgcctctcgc tcaatcagct
     6961 tattgatcaa gtcaaggccg agccctcgcc cccgttggga cgctgctacc ccgatttgcc
     7021 aaataaacaa cgtatcttgc tgtttaggag gaataaaagc ggtaataaat cctactactt
     7081 cgtcgccatc ttttgccaca atacatgtat cgtcaaaata ttcgcacatc ataatgtact
     7141 tgtaggacga gtttgtatcg agcgaagacg atttcgccag ttgccacatg gccgcgccat
     7201 cttccacggt tggctttgtt aacgttagta ctggtgtctg gaccataaca gcctcctttt
     7261 cgtttattat ttcgtttttg ccagacactg tttccttacc cgtctctcat cattctaaac
     7321 gttcgttttt gaaaaatttt atgaaagaac ataggcaact ttctttgcct tctataatga
     7381 taggggggcg tggcaacaaa tgcaaacgct taatcgttgg atataattgg attgatgtgg
     7441 ctgagtcgtg ctaagtcgag cagagagtag gctccttttc atatcctcct taaaagtcac
     7501 gtttccttcc atattcgcct tttgcttatg aaaaacaaaa aaaggagggt tccccctcct
     7561 ttcagcctgt cgccaagcgc ttgcatacgt cgtcgtttgg ctggactatg ctgactaccc
     7621 ctcaacctat tcccattttt tctcgtctac attttaaatc agtttgattg atgttactgt
     7681 cacacgcact gaaatgaagc aattcgctca gtgtcaatgc caaagaatac ccaacggaag
     7741 aaaaaccaac gccagcctgc tacagagcgc cggccgacaa aagtagggta aaaccaaaac
     7801 gattcgccgt tatttaggcg gataaacgta taacgaaaca ggcagcctgc gatcgctcca
     7861 ggatcaacag caaaaacaga aggcgcccct aaagtgctta aagccgcttg ggaaggaggc
     7921 gggccaggtg gcgcacccgt tccttgccct ggggaagtcg tcggggggcc tccctggcct
     7981 gggaaaaacc ctccttgtgt gggcgggggt ccaggtgggc caccttgtgt tggtggaaat
     8041 ggaaacgtgc ctgggctaaa gcgcattggg taatcatgcc cattgtacat aaacgagcaa
     8101 ctcctttttt ataacctttt tgctagccta cgcaactgcc catggaatgg tgaacaatat
     8161 ccattttttg tgtatagcat cgtcttaatt cctaatacta attggaaact aacaacagat
     8221 aaggaagaaa tggcatgagg acaacggatc aaaatgcatg tgctgcggcc attaaaagtg
     8281 ctttttacca atcaaacagc gtgcaaacac tggaaattcc gcttttagaa acagaccaag
     8341 cgcttgtgct attcattcaa gagattgtcg accaaaataa gttgcatgcg ttaatcgttg
     8401 atccccttac tcgtttccgt ctcgcaaagc agtcataccc gactgaatct gttacgactt
     8461 ggctgaaaga cggcaccccg ctaccaaatg ccactgtgga acaaataaaa cgggaaatga
     8521 tgaacggcca agcagttgta ttgttgaaaa acgaacagcc tgttttaatc gattgcgcga
     8581 tgtggaatgt ccgcagcgtc agcgtgcccc ttacaagcag cgtgtatgaa gggccagcat
     8641 cggctttgac ggaaagcctt gacgttaaca tgaatttgct ccgtaattat tttcgtagcc
     8701 cggaattagc gatcgaaaca ttagaagtcg gagaaaacgc tgcaaaaaaa atggcgattg
     8761 tctcgcttca cggaaaaacg aatccagacc ttgtcaaaga agtccgcgaa aggcttaaac
     8821 aagtgcatgt agccgaattc atcatttcgc aaattgcaac tgacgcgctc gaagggaaag
     8881 ggtttctgtt ccctcggaca atggcgaacg accgcccaga tgcgtgcgca ttggcattgg
     8941 caaaagggaa aattgtcttg ctcgtagaag gatcgccatt ggcgattctg gcaccaagcc
     9001 tgttttttca tttcttccaa aaccaagacg attacttatc cgaatttggc cgctttggcg
     9061 cacgtcctct tcggtatttg tattttttta ttgccacctt ttttccagct attactgtag
     9121 cgatcatccg ttttcatctc gatcacatac cgacggaagt ggctaaccag cttgtgaaaa
     9181 cccatgaaac ccttgccccc tatacaatcg agcttgtctt tgtcattctt ttattgcagc
     9241 ttattatgga tggctcctat cgcttgcctg gcaatacgat ttttgcagtg acgtttatcg
     9301 gcacaatgct cattagcgat attgctacaa atgttagcct attccatccg attaccatcg
     9361 tcgtcattgg cgtttgttat ataactagtt ttcctgtatt aaaccgcggg ctgctttctc
     9421 cgattttttt tattcgcata acgttgatta tcgtagccca tttctttggc tttgcaggaa
     9481 tgtttgctgt gacaaccgcg gtcatcatcc atatggccca tttaaggtcc cttggcattc
     9541 catatctcta tccgtttatt ccgtttcagc ctgataaatg gcatgacacg attttccgcc
     9601 ctactttgac gatggccatc aacaaaccga cgccgctgcc ttttaaaaaa gcagaggctg
     9661 cccagaatcg aagaggcagc tactgaggaa tgagatgaat taattgtggg atgaggcgaa
     9721 tctcacagca tcgcctttct ttaaataaca tatgattaaa ctacccattc ccgcggagga
     9781 atgggtggtt ctctttaaag ttgcaacgat atatatttca cttctacata ttcttcaatg
     9841 ccccaatggc caccttcacg gcctaggccg ctttctttca tgccgccgaa tggcgcttgg
     9901 gcgaccgaag gcaaaccatc attcagcccg acaatgccgt attcaagcct ttccgcaatc
     9961 gtaacagctt ccgataggtc atttgaatac acatatgctg ctaaaccgta aggggaatgg
    10021 ttggcccttt cgatcacgtc ttccgttgac gtgtacgtgg caatcggcgc aagcggtcca
    10081 aatgtttctt ccttcatgca aagcatgtca tcggtcgcat tggcaataag ggcaggcgtt
    10141 tggaagtagc cctttgtggc atcggcttct tggccaaaga ccacttcgcc tccttttgcc
    10201 tgtgcatcct caatatgctt ttgcactttt gcaaccgctg cttcgttaat aagagggcca
    10261 atgtggctgt ctggttccat gccgttgcct acttttagct gtttcatttc agcagcaaaa
    10321 gcctctacaa aaggcgcttt tatcgactcg tggacgtaaa tgcggtttgc acagacgcat
    10381 gtctgtccgc cattgcggta ctttg
//
